Instruction Manual - Cole
Contents
1. To start the flowcell pump press the red button on the Flow Thru panel on the front of the instrument Repeat as desired NOTE IT IS VERY IMPORTANT TO KEEP THE FLOWCELL SYSTEM CLEAN TO ENSURE A LONGERLIFETIME FOR THE FLOWCELL PUMP WATER INTO THE SYSTEM WHEN NOT IN USE I E OVERNIG 332. Cole Parmer 2100 2100UV SERIES SPEC TROPHOTOMETER USER S MANUAL Cole Parmer Instruments Company 625 East Bunker Court Vernon Hills IL 60061 800 323 4340 Table of Contents General Iniormaton ee INT OdUCTION LE ee ie ee Working lee UE el le e E Unpacking Instructions ee ee DEVS CR UEC UO is EDS ER ED N SG eee SE ER ae cea sh 2100 and 2100UV Spectrophotometer Operation Panel Model 2100UV Operation Panel Model 2100 Operation Panel Start up Self Calbratton ee ee ee ee ee ee ee Changing Curette Holders ee ee ee ee ee ee ee ren Basic Operation se issie N Rg sees vont gees ev kke ee ee Eer rrna Sample Preparation and Analysis Re ee ee ee ee ee ee ee Concentration Mode 28 RD ee Lu A Ni en nn satis Factor Mode eend oneal tn nn ns nt Pas ere Ne mue ee Output and Data Processing ee ee ee PHARES EES ENE ne et a Ce T en A a Application SoltWware RE ER EE eae net nt ones Maintenance ES ee be a Ee ee a Sans et Lamp Replacement ee ee ee ee ee ee ee Wavelength Calibration Check Absorbance Accuracy Check Ska LIE ING Clots lsc Ce cn acti ace oe owes al oe E or ah ae 2100 and 2100UV Partstist ee Troubleshooting ee ee ee ee ee ee Error Codes ES EE EL ag SE EE Ee et AHACNMONE En DNS GN GE EE General Information The spectrophotometer described in this manual is designed to be used by properly trained personnel in a suita
3. 10 or 100 and follow Step 1 to 4 to enter the new factor value You need to calculate the sample concentration by multiplying readout with the multiple 10 or 100 5 Read the concentration value of the sample direct from Data Display Screen 6 Insert a cuvette containing the next sample and read the result Repeat until all samples have been measured Operational Note If the MODE switch is changed to A or T then the concentration reading is frozen This requires the operator to re start at step 1 Output and Data Processing Printer Model 2100 and 2100UV have a RS232C Interface connector that can be connected to any RS232 printers provides RS232 printer p n S1100 206 as an optional accessory It requires a 9 pin null modem connection cable The RS232 Printer Setting should be as follows e Baud Rate 9600bps e Parity None e Data Bits 8 e Stop Bit 1 Application Software The Application Software 2100 is Windows based software designed to operate with Spectrophotometer Model 2100 and 2100UV Model 2100 and 2100UV uses its RS232C Interface connector to connect with a PC The software runs on a PC with Windows 95 98 Me NT 2000 XP operating system installed The Software offers two additional analytical methods Standard Curve and Absorbance vs Time Kinetics It performs the following methods for analysis e Absorbance Transmittance Concentration measure the Absorbance Transmittance
4. Cuvette Set of 4 glass Set of 2 quartz Set of 4 glass 4 position Cuvette Holder Manual 1 installed 1 installed Power Cord 1 1 User Manual 1 1 Installation 1 After carefully unpacking the contents check the materials with the packing list above to ensure that you have received everything in good condition 2 Place the instrument in a suitable location away from direct sunlight In order to have the best performance from your instrument keep it as far as possible from any strong magnetic or electrical fields or any electrical device that may generate high frequency fields Set the unit up in an area that is free of dust corrosive gases and strong vibrations 3 Remove any obstructions or materials that could hinder the flow of air under and around the instrument 4 Select either 230V or 125V 115V on the voltage selector switch on the back of the 2100 and 2100UV shown in Figure 2 to match your local main voltage supply 5 Turn on your 2100 and 2100UV and allow it to warm up for 15 minutes before taking any readings Inlet On Off Switch RS232 C Bidirectional Interface 115 230 Switch Power Supply Fan Figure 2 Back of 2100 and 2100UV 2100 and 2100UV Spectrophotometer Operation Panel Model 2100UV Operation Panel Figure 3 shows the Model 2100UV and its Components e Sample Compartment Accommodate different cuvette holders 4 position Cuvette Holder is preinstalled in
5. or defective Check sample holder Replace lamp Lamp is off alignment Refer to lamp replacement instructions in this manual Defective electronic component Call an authorized service engineer T cannot be set to 00 0 T Sample holder Remove cuvette holder or test tube Sample holder shutter May be stuck open Close shutter Defective electronic component Call an authorized service engineer Incorrect Transmittance to Absorbance Bubbles or particles in solution Check sample preparation and analytical procedure Defective electronic component Call an authorized service correlation engineer Digital Display Concentration reading frozen MODE switch has been does not changed from C to F TorA change and back to C Restart regardless of measurement procedure sample Wrong wavelength setting Check sample procedure concentration and wavelength setting Insufficient sample volume Fill cuvette with more samples Stray sample preparation vapors Prepare the sample away from the instrument Use proper ventilation Bubbles or particles in solution Check sample preparation and analytical procedure Defective electronic component or loose wiring Call an authorized service engineer 23 PROBLEM Possible Cause Solution Instrument drift No sufficient warm up time Check lamp has been and noise Significant temperat
6. the needs of the conventional laboratory 2100 and 2100UV are ideal for various applications such as Clinical Chemistry Biochemistry Petrochemistry Environmental Protection Food and Beverage Labs Water and Waste Water Labs and other fields of quality control Featuring a digital display of photometric result easy operation and wavelength range of 325 1000 nm Model 2100 or 200 1000 nm Model 2100UV 2100 and 2100UV are ideal for measurements in the visible wavelength region of the electromagnetic spectrum ultraviolet and visible for the Model 2100UV Working Principle The spectrophotometer consists of five parts 1 Halogen Deuterium Lamp to supply the light 2 A Monochromator to isolate the wavelength of interest and eliminate the unwanted second order radiation 3 A Sample Compartment to accommodate the sample solution 4 A Detector to receive and convert the transmitted light to an electrical signal 5 A Digital Display to show absorbance or transmittance Figure 1 illustrates the relationship between these parts A d 100 T lt gt S Zei P on Light Source Monochromator Sample Compartment Detector Digital Display Figure 1 Block Diagram for the Spectrophotometer In your spectrophotometer light from the lamp is focused on the entrance slit of the monochromator where the collimating mirror directs the beam onto the grating The grating 4 disperses the light beam to produce the spectrum a portio
7. the wavelength setting reaches 370 nm 10 Look for the maximum absorbance reading obtained and this should be found between 359 and 363 nm The wavelength accuracy of the 2100 is 2 nm Didymium Filter Method 1 Set the Wavelength to 800 nm 2 Make sure the cuvette holder is empty and place it in the Sample Compartment Close the lid 3 Set zero Abs by pressing the 0A 100 T button Wait a few seconds while the display flashes BLA The Data Display Screen should then be 0 000A If not repeat step 3 Remove the cuvette holder and insert the Didymium filter into it Place it in the Sample Compartment and close the lid Record the Absorbance reading on the Data Display Screen Advance the wavelength setting by 1nm and repeat step 2 to 5 Repeat step 6 until the wavelength setting reaches 815 nm Look for the maximum absorbance reading obtained and this should be found between 805 and 809 nm The wavelength accuracy of the 2100 is 2 nm 9 If a middle wavelength check is desired set the wavelength to 522 nm optional 10 Make sure the cuvette holder is empty and place it in the Sample Compartment Close the lid 11 Set zero Abs by pressing the 0A 100 T button Wait a few seconds while the display flashes BLA The reading should then be 0 000A If not repeat step 11 12 Remove the cuvette holder and insert the Didymium filter into it Place it in the Sample Compartment and close the lid 13 Record the absorban
8. 0 but preset at 220 V S 2100UV Model 2100UV Spectrophotometer 5 nm Bandpass Wavelength range 200 1000 nm automatic wavelength change Voltage preset at 110V Complete with 4 position Cuvette Holder 4 matched Optical Glass Cuvettes 2 Quartz Cuvettes RS 232C Port Dust Cover User Manual S 2100UV E Same as Model 2100UV but preset at 220 V Software S 2100 401 Application Software for PC s Window s 95 98 or above Required Programs include Standard Curve Abs T Conc and Abs vs Time Includes S 2100 226 serial cable Accessories S 2100 101P Test Tube Holder Package for 8 to 20 mm diameter test tubes Includes Universal Base one holder and Top Hat for up to 150 mm tall test tubes S 2100 102P Rectangular Long Path Cuvette Holder Package for cells up to 100 mm pathlength Includes Universal Base and one holder S 2100 103P Single 10 mm Cuvette Holder Package Includes Universal Base amp one holder S 2100 104P Cylindrical Cuvette Holder Package for cells up to 100 mm pathlength 22 mm diameter Includes Universal Base and one holder S 2100 105P Water Jacketed Single Cuvette Holder Package for 10 mm cuvettes for Temperatures from 0 95 C Includes Holder Universal Base and Flow thru Panel Water Bath Required S 2100 106P Micro Cuvette Holder Package for use with 10 mm microcuvettes Includes adjustable x y base and single Cuvette Holder for 10 mm square cuvette S 2100 107
9. 00UV Parts List continued Catalog Description Accessories S 2100 111 Manual 4 position Cuvette Holder included with 2100 and 2100UV S 2100 112 Universal Base Accommodates up to 2 same or different type of holders S 2100 113 Top Hat for test tubes with height of 75 150 mm S 2100 114 Spacers for 5 mm Cuvettes S 2100 115 Holmium Oxide Filter S 2100 116 Didymium Filter S 2100 120 Haake Model DC10 B3 water bath for temperatures from 25 100 C For use with S 2100 105 S 2100 121 Haake Model DC10 K10 water bath for temperature ranges of 10 100 C For use with S 2100 105 Output Device S 1100 206 Printer requires S 1100 207 printer cable S 1100 207 Printer Cable Male 25 pin to Female 9 pin Null Modem S 2100 226 RS 232C Cable 9 pin to 9 pin Female Female Null Modem Glassware S 90 301 Test Tube Cuvettes 10 mm Diameter 12 pcs S 90 302P Disposable Cuvettes Polystyrene 10 mm Pathlength 100 pcs 100 S 90 302P Disposable Cuvettes Polystyrene 10 mm Pathlength 500 pcs 500 S 90 305P Semi micro Disposable Cuvettes Polystyrene 4 mm window 500 pcs S 90 304G Square Cuvettes Optical Glass Set of Two S 90 309Q Square Cuvettes Quartz 10 mm Set of Two S 90 308 50G Cylindrical cell 50 mm pathlength S 90 326G Rectangular cell Optical glass 50 mm pathlength with stopper S 90 344FG Flowcell Optical glass 4x12 mm
10. Concentration Standard or Concentration Factor at a single wavelength within the range of 325 1000 nm 200 1000 nm for 2100UV e Standard Curve create a calibration curve choice of 4 curve fits with up to 8 standard solutions at a single wavelength to determine concentrations of unknown samples Absorbance vs Time Kinetics measure a sample s absorbance change over a selected period of time store the test results in data table and display the results graphically Refer to the 2100 Series Spectrophotometer Software User s Manual Version SB 2 10 for detail 16 Maintenance Lamp Replacement A Replace Tungsten Halogen Lamp 1 Turn off and unplug the instrument 2 Remove the four screws on the sides of the spectrophotometer 3 Remove the Cuvette Holder Control Knob by unscrewing the rod counterclockwise 4 Remove the cover of the instrument very carefully and place it in front of the instrument HINT Lift up about 3 4 inches and once the instrument has cleared the backside grill plate lift the back of the cover up towards the front The front of the instrument will act as a hinge BE SURE TO NOT PULL PANEL WIRING LOOSE 5 Unplug and remove the lamp from ceramic base the white connector Insert the new lamp pushing it in as far as it will go Part Number S2100 515 6V 10W G4 type CAUTION DO NOT HANDLE THE LAMP WITH BARE FINGERS USE TISSUE OR CLOTH WHEN HANDLING LAMP 6 Turn on the instrument Set the wa
11. LOOSE 17 5 Remove the metal plate covering the Deuterium lamp by removing the two screws from the rear of the plate make a note of the wire color and their matching connectors 6 Remove the lamp by disconnecting the 3 wire connector and pulling straight up on the lamp socket DO NOT PULL on the lamp itself This may cause the lamp to break 7 Replace the pre aligned lamp with a lamp provided by or an authorized Service Provider Call 1 800 588 9776 for details This comes pre assembled with lamp socket 8 Reconnect the wire connector and be sure the lamp and lamp socket is securely in place make sure the wire connection orientation is the same as step 5 9 Turn on the instrument After the Start up Self Calibration select 300 nm and press 0A 100 T Button Check to make sure that the light beam is focused on the entrance slit of the monochromator If it is not adjust the mounting screws on the Deuterium lamp holder to align the lamp Note The latest version of Model 2100 2100UV has a lamp access door at the top of the instrument If your Model 2100 2100UV has the lamp access door you do not need to open the cover Just open this door to replace the lamp CAUTION THE LAMP MAY BE HOT TAKE PRECAUTIONS TO PREVENT POSSIBLE BURNS Wavelength Calibration Check Normally the 2100 Series spectrophotometer retains its wavelength calibration indefinitely However if the instrument receives a severe shock or is abused use the followin
12. P Peltier Package for continuous temperature control from 15 to 40 C Includes p n S 2100 107A peltier thermal electric 10 mm Cuvette Holder and p n S 2100 107B peltier controller unit S 2100 108P Flow thru Package for continuous temperature control from 15 to 40 C Includes p n S 2100 109 Flow thru controller and p n S 2100 108B flow thru panel requires flowcell and tubing S 2100 109P Peltier Zipper Package for flow through and continuous temperature control from 15 to 40 C Includes p n S 2100 109 peristaltic flow thru controller and p n S 2100 107A Peltier Cuvette Holder with panel requires flowcell and tubing S 2100 101 Test Tube Holder without base Reduires Universal Base S 2100 102 Rectangular Long Path Cuvette Holder without base requires universal base S 2100 103 Single 10 mm Cuvette Holder without base requires universal base S 2100 104 Cylindrical Cuvette Holder without base Requires Universal Base S 2100 105 Water Jacketed Single Cuvette Holder with flow thru panel but without base Requires Universal Base Water Bath S 2100 107A Peltier Thermal Electric 10 mm Cuvette Holder with panel S 2100 107B Peltier controller unit for controlling temperature from 15 to 40 C S 2100 108A Flow thru panel for use with ambient flowcell system requires flowcell tubing and p n S 2100 109 flow thru peltier controller S 2100 109 Peristaltic Pump Peltier Thermal Temperature and Flow thru Controller Only 21 2100 and 21
13. Sample Compartment and close the lid Read the absorbance of the standard from the Data Display Screen The value should be 1 288 0 02 A Refer to the notes above when interpreting the result Stray Light Check Specification Less than 0 3 T at 340 nm by ASTM E 387 A good indication as to whether the stray light level is within specification may be obtained as follows Set the wavelength to 340 nm Set the MODE button to T With the Sample Compartment empty close the lid and press the 0A 100 T button to set the display to 100 0 Hold the MODE key for at least 5 s This will set the 0 00 T This may take a few minutes Prepare a solution containing 50 gm L of sodium nitrite NaNOz in distilled water and fill a square cuvette with this solution Insert the cuvette into the Sample Compartment and close the lid The Data Display Screen display should read lt 0 3 T If the reading obtained in step 4 is greater than 0 00 it should be subtracted from the displayed Data Display Screen to give the correct reading for the stray light value 20 2100 and 2100UV Parts List Table 4 2100 an d 2100UV Parts List Catalog Description S 2100 Model 2100 Spectrophotometer 5 nm Bandpass Wavelength range 325 1000 nm automatic wavelength change Voltage preset at 110V Complete with 4 position Cuvette Holder 4 matched Optical Glass Cuvettes RS 232C Port Dust Cover User Manual S 2100 E Same as Model 210
14. ach sample Remove the sample cuvette s 11 If you are to test the same sample at other wavelengths repeat step 3 to 10 for each wavelength 12 For each new sample you analyze repeat step 2 to 11 14 Concentration Mode C is used for determining the concentration of unknown samples NOTE This method should only be used when the relationship between Absorbance and Concentration is known to be linear The concentration of the Standard solution used to calibrate the instrument should be higher than the most concentrated sample 1 Select the desired wavelength by pressing Wavelength Control buttons A and V turning the wavelength control knob Using the MODE button select A mode Insert the cuvette containing the blank solution Set 0 000A with the 0A 100 T button Using the MODE button select C mode Insert a cuvette containing a standard solution of known concentration in the first position of the Sample Compartment and set the Data Display Screen to be the value of the standard by using the INC and DEC buttons 7 Press the ENT button ol IM NOTE If the reading changes the factor required is too high i e gt 1999 to be displayed In this case divide the concentration by 10 re select the C mode by successive presses on the MODE button cycling through the F T and A modes and follow step 2 above to set the concentration of the standard to the reduced value 8 With the standard concentration set determine the c
15. ating Step 4 to 6 in reverses Screw in your holder to any of the open four positions on the holder NOTE If a second holder is purchased i e to make into a 2 position Cuvette Holder place the second holder in a position so one position is skipped For example if the first holder is in position one 26 and then the second must be in either position 3 or 4 of the Universal Holder Two same type or different types of holders can be installed on the universal platform Water Jacketed Single Cuvette Accessory Installation Guide S 2100 105P Step 1 Remove all items from the accessory package made sure that they are complete refer to Figure 14 for S 2100 105P Universal Platform 1 pc Water Jacketed Holder 1 pc Flow thru Panel 1 pc Installation Guide 1 pc Step 2 The only tool needed is a standard Philips head screwdriver Step 3 Remove the 4 position Cuvette Holder Open the Sample Compartment cover and remove any cuvettes or any breakable items Push in the Cuvette Holder Control Knob black knob all the way back First position Cuvette Holder is into the light path Step 4 Unscrew the Cuvette Holder rod by turning the knob counterclockwise until removed from holder Gently slide the guide rod out of the Sample Compartment Step 5 Looking over the Sample Compartment remove the front screw Figure 15 Step 6 To remove the back screw place your hand ON TOP of the 4 position Cuvette Holder and gently slide towards
16. ble equipped laboratory For the correct and safe use of this spectrophotometer it is essential that laboratory personnel follow generally accepted safe procedures in addition to the safety precautions called for in this manual The inside of the power supply unit is a hazardous area and its cover should not be removed under any circumstances ANY Servicing must be done by an authorized person Some of the chemicals you use in the spectrophotometer may be corrosive flammable radioactive toxic and or potentially infective Care should be taken to follow the normal laboratory procedures for handling chemicals and samples Please carefully read the Safety Electrical Warning Performance and Radio Interference instructions below Safety This spectrophotometer has been designed and tested in accordance with EN 61326 1 1997 Safety Requirements for Electrical Equipment for Measurement Control and Laboratory Use standard EMC Requirements The spectrophotometer has been supplied in a safe condition The safety statements in this manual comply with the requirements of the HEALTH AND SAFETY AT WORK ACT 1974 Read the following before installing and using the instrument and its accessories Electrical Before switching on the spectrophotometer make sure it is set to the voltage of the local power supply see Installation The main plug shall be inserted in a socket provided with a protective earth contact The protective action must not b
17. ce reading on the Data Display Screen 14 Advance the wavelength setting by 1nm and repeat step 10 to 13 15 Repeat step 14 until the wavelength setting reaches 536 nm Again look for the maximum absorbance reading It should be between 527 and 531 nm gt A ONO Absorbance Accuracy Check Specification 0 5 at 1A 1 at 2A The absorbance accuracy should be checked against a set of neutral density filters accurately calibrated to the NIST standards Contact your representative for more information 1 800 588 9776 An alternative method using potassium dichromate is described below Due to the many factors that might affect the results i e temperature bandpass weighing and diluting errors this method is less accurate and should only be used as a guide Reference Johnson E A Potassium Dichromate as an absorbance standard PSG Bulletin 1967 No 17 page 505 1 Make up N 100 sulfuric acid as the solvent and use part of it to make a solution 19 N OO Ee N SE containing 120 0 5 mg liter of potassium dichromate Wash out a square cuvette with solvent and fill with solvent Put the cuvette in the adapter into the Sample Compartment and close the lid Set the wavelength to 350 nm Set the MODE button to A Set the reading on the Data Display Screen to 0 000A using the 0A 100 T button Empty the cuvette Wash out with dichromate solution and fill with dichromate solution Put the cuvette in the adapter into the
18. counterclockwise until centered gt Ifthe light is too low centered on cuvette but light beam is focused below window turn the adjustment screw clockwise until centered A simple way of doing this is to monitor the transmittance reading on the display while adjusting the screw The best position is that you get maximum transmittance reading on the display This mean that maximum light beam energy passes through the cuvette If the light is left or right of the center of the cuvette then the x axis will need adjustment For the x axis adjustment turn the white adjustment knob The white adjustment knob is located at the front right of the holder Figure 19 gt Remove the Front Panel Figure 16 gt Ifthe light is to the back of the instrument turn the knob clockwise until centered gt Ifthe light is towards the front of the instrument turn the knob counterclockwise until centered Once again the best way is to monitor the transmittance reading on the display while adjusting the knob Turn the knob clockwise or counterclockwise until you get the maximum transmittance reading on the display This indicates that light beam is centered to the cuvette window to the maximum energy Micro cell Holder Front View y axis fine aard adjustment 2 x axis adjustment knob Figure 18 Micro Cuvette Holder Front View 29 Light Beam e Greg x axis Guide adjustment knob K Micro cell holder right side view Figu
19. dary filters lamp selection and positions secondary filters P2 Monochromator locates starting position Monochromator locates starting position P3 Monochromator locate 0 order light Monochromator locate 0 order light and and measure initial light energy measure initial light energy P4 e Monochromator go to 546 nm e Monochromator goes to 546 nm e Instrument sets O T e Initiates RS 232C port Show PC CONN e Set 100 T blank reference e Instrument sets OXT e Initiates RS 232C port Show PC CONN e Set 100 T blank reference Figure 7 P 2 Process Figure 8 P 3 Process Figure 9 P 4 Process URAC G 7546 Figure 10 Show Process Figure 11 PC CONN Process Figure 12 Normal Start up Screen Changing Cuvette Holders There are seven different Cuvette Holders Sample Holders refer to Figure 13 and Figure 14 from left to right S2100 101P S2100 102P S2100 103P S2100 104P S2100 105P S2100 106P S2100 109P Test Tube Holder for 8 25 mm diameter test tubes Includes Universal Base one holder and Top Hat for up to 150 mm tall test tubes Rectangular Long Path Cell Package for cells up to 100 mm pathlength Includes Universal Base and one holder Single 10 mm Cuvette Holder Package Includes Universal Base and one holder Cylindrical Cuvette Holder Package for cells up to 100 mm pathlength 22 mm diameter Includes Universal Base and one holder Water Jack
20. diameter Outlet tubing from the flowcell to the outlet connector This tubing is usually the same size as the tubing from the outlet connector to the peristaltic pump The length should be no less than 10 cm for each section of tubing The Peltier Flowcell Accessory is now ready for testing Figure 23 Inlet pipe Inlet Tubing Outlet Tubing Tubing to pum LI ENS Panel Panel l Pump Waste Sample Flowcell Figure 23 The Liquid Flow Chart 32 USING THE PELTIER FLOWCELL ACCESSORY Step 15 Step 16 Step 17 Turn on the spectrophotometer if power is off and Peltier Flowcell Accessory Select the temperature 25 30 or 37 C on the Controller To do this push the Mode button on the Controller so the temperature light is illuminated Select the temperature using the up and down buttons Figure 24 Peltier Flowcell Accessory Front View Temperature Indicator S Button Flowcell Indicator Readout Figure 24 Peltier Flowcell Accessory Front View Press the Mode button on the Controller The number showing is the number of seconds the peristaltic pump on the flowcell system will operate To adjust this use the up and down buttons PLEASE NOTE Since sample size and desired volume varies from sample to sample the user must experiment to find the optimum pump time If less volume to be used is desired then decrease the Flowcell pump time If more volume is needed then increase the time
21. e negated by the use of an extension cord without a protective conductor Warning Any interruption of the protective conductor inside or outside the spectrophotometer or disconnection of the protective earth terminal is likely to make the spectrophotometer dangerous Intentional interruption is prohibited Whenever it is likely that the protection has been impaired the spectrophotometer shall be made inoperative and be secured against any unintended operation NEVER touch or handle the power supply on the Model 2100UV due to the high voltage The protection is likely to be impaired if for example the apparatus e Shows visible damage e Fails to perform the intended measurements e Has been subjected to prolonged storage under unfavorable conditions 3 e Has been subjected to severe transport stresses Performance Carry out performance checks with particular reference to wavelength and absorbance accuracy to ensure that the spectrophotometer is working within its specification especially when making measurements of an important nature Performance checks are detailed in this manual Radio Interference For compliance with the EMC standards referred to in the EC Declaration of Conformity it is necessary that only shielded cables supplied by are used when connecting the instrument to computers and accessories Introduction The Model s 2100 and 2100UV Spectrophotometers are single beam general purpose instruments designed to meet
22. eted Single Cuvette Holder Package for 10 mm cuvettes for temperatures from 0 95 C Includes Holder Universal Base and Flow thru Panel Water Bath Required Micro Cuvette Holder Package for use with 10 mm microcuvettes Includes adjustable x y base and holder Peltier Flowcell Package for 15 C to 40 C Includes p n S2100 109 peristaltic temperature pump flow thru controller p n S2100 107A Peltier Thermal Electric Cuvette Holder and Peltier Flow thru Refer to the Attachment in this Manual for the detailed Installation Guide The only tool needed is a standard Philips head screwdriver Figure 13 Cuvette Holder S2100 101P to S2100 104P Figure 14 Cuvette Holder S2100 105P 106P and 109P 13 Basic Operation Three Basic Operations Sample Preparation and Analysis Concentration Mode C and Factor Mode are shown in this section Sample Preparation and Analysis A Spectrophotometer Warm up and T Check 1 Turn on the spectrophotometer by turning on the Power Switch IO Allow 15 minutes for the instrument to warm up Operational Note The instrument performs four self calibration checks every time the power is turned on For details of the self calibration and the error codes associated with this please see the Error Codes section of this manual 2 Select either the T Transmittance Mode or A Absorbance Mode by pressing the MODE button until the red light for T or A is on 3 Select the desired wavelength by
23. filter 2100 Open cover to instrument and check CPU board connections Lamp change over motor or lamp initialization switch malfunctioning 2100UV Filter driven motor malfunctioning 2100 Call authorized service engineer ERR 02 Monochromator unable to locate Monochromator contact switch or grating starting position 2100 or driven motor is malfunctioning 2100 initialize secondary filter will not Filter driven motor malfunctioning 2100UV initialize 2100UV Call authorized service engineer ERR 03 Instrument unable to locate 0 Monochromator contact switch or grating order light 2100 and 2100UV driven motor is malfunctioning 2100UV or monochromator unable to Check tungsten halogen lamp Replace locate starting position 2100UV lamp if necessary both Monochromator contact switch loose or mis positioning both Check deuterium lamp 2100UV WEAR UV protective eyewear Tungsten halogen or deuterium Check lamps by opening cover as instructed lamp is misaligned or no longer in this manual See Maintenance functioning Replace lamps if necessary Contact an authorized service engineer ERR 04 Instrument is unable to locate Check sample compartment and remove 546nm and automatically set O T and 0A 100 T cuvette or any object blocking light path Place the 4 cell sample holder in the proper position Contact an authorized service engineer 25 Attachment Refer the following attachments for the Accessory Installati
24. g methods to check wavelength calibration Please note that this test requires the Didymium filter p n 2100 116 or the Holmium Oxide filter p n S2100 115 In the filter method the didymium filter has two distinct absorbance peaks at 529 nm and 807 nm The Holmium filter has a distinct peak at 361 nm When the instrument is calibrated properly you will find minimum Transmittance maximum Absorbance at the range 2 nm from these peaks Note that the specific Transmittance values are not important as you are only looking for the wavelength where the minimum Transmittance maximum Absorbance occurs Note If you calibration filter has a certified peak valley curve attached please use the peaks on the curve to verify the instrument Holmium Oxide Filter Method 1 Turn instrument on and allow it to warm up for 15 minutes 2 Select the A mode 3 Set the wavelength to 350 nm 4 Make sure the cuvette holder is empty and place it in the Sample Compartment Close the lid 5 Set zero Absorbance by pressing the 0A 100 T button Wait a few seconds while the display flashes BLA The reading on the Data Display Screen should be 0 000A If not repeat step 5 6 Remove the cuvette holder and insert the Holmium filter into it Place it in the Sample Compartment and close the lid 18 7 Record the Absorbance reading on the Data Display Screen 8 Advance the wavelength setting by 1 nm and repeat step 4 to 7 9 Repeat step 8 until
25. he instrument Insert Flow thru Panel for Peltier Flowcell Accessory in place of removed panel Connect Peltier Flowcell Controller to the Outlet Tube Connector Figure 20 Flow thru Panel for Peltier Flowcell Accessory Start Button Outlet Connector RS 232 Cable Inlet Connector for Peltier Black tube Figure 20 Flow thru Panel for Peltier Flowcell Accessory Connect the 9 PIN Cable from the Flow Thru Panel to the back of the Peltier Flowcell Controller Figure 21 Back View of Peltier Flowcell Controller Power Switch Power Cord Outlet RS 232 Plug for Flow thru Panel Figure 21 Back View of Peltier Flowcell Controller 31 Step 13 Connect the tubing from the outlet connector of the Flow Thru Panel to the Peltier Flowcell Controller Figure 22 Push the tubing into one of the two locking positions on both the Front and Back side of the Peristaltic Pump Front Back To Waste ee ay Container Peristaltic Pump Peltier Flowcell Controller Side View Figure 22 Peltier Flowcell Controller Side View Step 14 The external components are ready Now with tubing supplied by either the manufacturer or the user connect the flowcell to the Flow Thru Panel Two different tubing will be needed inside the Sample Compartment Inlet tubing from inlet connector pipe to the flowcell Depend on the type of flowcell the inlet tubing may have a cap with thread The inlet tubing is usually smaller in
26. into the light path Unscrew the Cuvette Holder rod by turning the knob counterclockwise until removed from holder Gently slide the guide rod out of the Sample Compartment Looking over the Sample Compartment remove the front screw Figure 15 To remove the back screw place your hand ON TOP of the 4 position Cuvette Holder and gently slide towards the front The back screw is then exposed and can be removed Place the x y adjustable base into the Sample Compartment by aligning the two alignment pins parallel with the light beam with the two holes on the bottom of the x y base Tighten the rear screw It may not be necessary to replace the front screw since the alignment pins will properly place the base 28 Adjusting the Micro Cuvette Holder Step 8 Step 9 Step 10 Step 11 Please turn on the power to the instrument if the unit is off After self calibration the spectrophotometer will stop at 546 nm green light Place your micro cell cuvette into the cuvette holder The light beam should be in the center of the cuvette If the green light is centered you are ready to make your measurements If the green light is not centered on the cuvette please proceed to Step 10 You will need a basic screwdriver for the y axis adjustment The adjustment screw is located in the front center of the holder Figure 18 gt Ifthe light is too high centered on cuvette but not optimizing minimum volume turn the adjustment screw
27. ment until the base is engaged into two hex head guiding pin Lock the base with the Philips type screws Step 8 Align the Cuvette Holder so that maximum light will pass through the Cuvette Holder This can be achieved by adjusting the Cuvette Holder horizontally and vertically with two setting knobs 30 Step 9 Step 10 Step 11 Step 12 a Horizontal setting There is one knob located in front right of the Cuvette Holder base for horizontal backward or forward adjustment Turning this knob clockwise will push the Cuvette Holder backward and counterclockwise will push the Cuvette Holder toward you Turn on the spectrophotometer and MONITOR the transmittance reading on the display Locate the maximum transmittance reading on the display by turning the knob and this should be the best horizontal Cuvette Holder setting b Vertical setting There is a long crew on top right behind the Cuvette Holder for vertical position adjustment Turn this screw clockwise will raise the Cuvette Holder and vise visa Locate the maximum transmittance reading and it should be the best vertical Cuvette Holder setting Generally speaking the Cuvette Holder should be almost at its lowest position The Z dimension is 15 mm Minor adjustment may be required if micro flowcell is used after such flowcell is installed Place Flowcell into the Cuvette Holder Be sure to align the windows of the flowcell with the horizontal light path of t
28. n of which is focused on the exit slit of the monochromator by a collimating mirror From here the beam is passed to a sample compartment through one of the filters which helps to eliminate unwanted second order radiation from the diffraction grating Upon leaving the sample compartment the beam is passed to the silicon photodiode detector and causes the detector to produce an electrical signal that is displayed on the digital display Specifications Table 1 lists the specification for both Model 2100 and 2100UV Table 1 Specifications Model 2100 Model 2100UV Wavelength Range 325 1000 nm 200 1000 nm Spectral Bandpass 5 nm Wavelength Accuracy Better than 2 nm Wavelength Repeatability Better than 1 nm Stray Radiant Energy lt 0 3 T at 340 and 400 nm Photometric Range 0 125 T 0 1 2 5 Abs 0 1999 C 0 1999 Factor Photometric Accuracy 0 004 Abs at 0 5 Abs Power Requirements 115 230 V 10 60 50 Hz switchable Sample Holder 4 poisiton Cuvette Holder Dimensions 470W x 400D x 140H mm Net Weight 10 kg 22 Ibs 12 kg 27 Ibs Unpacking Instructions Carefully unpack the contents and check the materials against the following packing list to ensure that you have received everything in good condition Packing List Table 2 Packing List Description Quantity Model 2100 Model 2100V Spectrophotometer 1 1 Dust Cover 1 1
29. o select the desired wavelength with the A and V increase and decrease buttons INC DEC Concentration Factor Buttons Allow user to increase or decrease the displayed Concentration at C mode or Factor number at F mode ENT Button First press at C mode enters reading on Data Display Screen Other presses send displayed results to printer If using Windows based Application Software press this button to communicate with the software refer to the 2100 Series Spectrophotometer Software User s Manual Version SB 2 10 for details If operating in F Mode then press to enter factor This causes the Mode to enter the factor number and change to C mode TRANSMITTANCE ABSORBANCE CONCENTRATION FACTOR f WAVELENGTH 3 LA Figure 5 Operation Panel of Model 2100 Start up Self Calibration The Model 2100 and 2100UV have self calibration programs that begin when the instrument is powered on The following are the steps taken automatically each time the instrument is switched on Each step is displayed on the Wavelength Display Screen Figure 6 to Figure 12 and Table 3 show the detailed information of the Start up Self Calibration P1 Process P2 Process P3 Process P4 Process Show Process PC CONN Process and Normal Start up Screen Figure 6 P 1 Process 10 Table 3 Start up Self Calibration Display 2100 2100UV P1 Check the electronic components and Check electronic components initiates positions secon
30. ollowing components e Sample Compartment Accommodate different cuvette holders 4 position Cuvette Holder is preinstalled in the standard package e Power Indicator Indicate whether power is on or off e Cuvette Holder Control Knob Select the sample position of the 4 position Cuvette Holder by pulling out or pushing in e Operation Panel has nine components see below for details Figure 5 illustrated the detailed information of the Operation Panel 1 Data Display Screen Display the data of Transmittance Absorbance Concentration and Factor modes Mode Indicator The red LED light indicates the operation MODE Allow the operator to know the measurement mode currently in use T Transmittance A Absorbance C Concentration and F Factor 0A 100 T Button Blank the 2100 2100UV when blank reference solution is in the Sample Compartment Every time a new wavelength is selected press 0A 100 T Button to confirm It will automatically blank the 2100 2100UV MODE Button There are four modes T mode is transmittance mode A mode is absorbance mode C mode is to measure unknown sample concentration through a standard solution F mode is to measure unknown value with a previously determined factor PRINT Button Press to send Data Display Screen and Wavelength Display Screen results to RS 232C port and printer Wavelength Display Screen Display the current desired wavelength Wavelength Control Buttons Allow user t
31. on Guide Universal Holder and Accessory Installation Guide 2100 101P to 2100 104P Water Jacketed Single Cuvette Accessory Installation Guide 2100 105P Micro Cuvette Holder Package Accessory Installation Guide 2100 106P Peltier Flowcell Accessory Installation Guide 2100 109P Universal Holder and Accessory Installation Guide S 2100 101P to S 2100 104P Step 1 Step 2 Step 3 Step 4 Step 5 Step 6 Step 7 Step 8 Remove all items from the accessory package and make sure that they are complete refer to Figure 13 for the S 2100 101P to S 2100 104P A Universal Platform 1 pc The specified holder 1 pc Installation Guide 1 pc The only tool needed is a standard Philips head screwdriver Remove the 4 position Cuvette Holder Open the Sample Compartment cover and remove any cuvettes or any breakable items Push in the Cuvette Holder Control Knob black knob all the way back First position is into the light beam Unscrew the Cuvette Holder rod by turning the knob counterclockwise until removed from holder Gently slide the guide rod out of the Sample Compartment Looking over the Sample Compartment remove the front screw Figure 15 Standard 4 cells Overhead View Front Figure 15 Remove Front Screw To remove the back screw place your hand ON TOP of the 4 position Cuvette Holder and gently slide towards the front The back screw is then exposed and can be removed Insert Universal Holder by repe
32. oncentration values of samples with unknown concentration by inserting the sample cuvette into the Sample Compartment and reading the value direct from the Data Display Screen 9 To read the value of the multiplier used to convert Abs to Concentration after measuring all the samples change the mode to F and read the multiplier from Data Display Screen Keep a record of this value for future use Operational Note if the mode switch is changed to read F or A the Concentration C reading is frozen and cannot be changed This requires the operator to re start at step 1 Factor Mode This is a special mode for measuring concentration values of unknown samples using a previously determined factor to convert absorbance readings to concentration 1 After setting the wavelength and setting zero Abs on the blank solution using the MODE button select F mode 2 Insert a cuvette containing a sample 3 Using the INC and DEC buttons set the Data Display Screen to the desired value of the multiplier 4 Press the ENT button The spectrophotometer switches to the C mode Operational Note If the Concentration of the sample is too high to be displayed the instrument will not switch to C mode when the ENT button is pressed Dilute the sample and multiply the concentration reading by the dilution factor to obtain the original sample 15 concentration If dilution is impossible or causes other problems you may divide the factor value by
33. or Buttons and Lamp Control Buttons Allow user to increase or decrease the displayed Concentration at C mode or Factor number at F mode At T mode or A mode press INC button will shutdown the halogen lamp W press DEC mode will shutdown the deuterium lamp D2 To turn on the lamps just press the INC DEC button again ENT Button First press at C mode enters reading on Data Display Screen Other presses send displayed results to printer If using Windows based Application Software press this button to communicate with the software refer to the 2100 Series Spectrophotometer Software User s Manual Version SB 2 10 for details If operating in F Mode then press to enter factor This causes the Mode to enter the factor number and change to C mode Visible Lamp Indicator W n the operating mode of Absorbance or Transmittance A or T pressing the INC button will turn off on the lamp UV Lamp Indicator D2 n the operating mode of Absorbance or Transmittance A or T pressing the DEC button will turn off on the lamp Since Deuterium lamp D2 is an expensive item it is recommended to turn off the when not using ultraviolet light for measurement Sample Compartment 4 Cell Manual Power eN Sample Holder Control Knob Operation Panel Figure 3 Model 2100UV Spectrophotometer Figure 4 Operation Panel of Model 2100UV Model 2100 Operation Panel The Model 2100 has the similar outlook as Model 2100UV It also has the f
34. pressing the appropriate Wavelength Control buttons and V At any time if the wavelength is changed the Data Display Screen will display BLA This serves as a reminder that a reference is necessary with the change of the wavelength and you need to push 0A 100 T Button to blank Sample Preparation Make a blank reference solution by filling a clean cuvette or test tube half full with distilled or de ionized water or other specified solvent Wipe the cuvette with tissue to remove the fingerprints and droplets of liquid 5 Insert the blank cuvette into one cell of the 4 position Cuvette Holder Push or pull the Cuvette Holder Control Knob so that the cuvette is in the light path Close the cover 6 Set 0 000A or 100 T with the 0A 100 T Button 7 Remove the blank cuvette if you are testing more than three samples Set it aside in the case that you may need to adjust the 0A 100 T button later i e change the wavelength AW C Sample Analysis 8 Rinse a second cuvette with a small amount of the sample solution to be tested Fill the cuvette half full and wipe it 9 Put the sample cuvette in the Sample Compartment Close the cover 10 Read the T or A from the Data Display Screen If you are reading more than one cuvette be sure to carefully move the stage to the next position by pulling on the Cuvette Holder Control Knob until you feel the holder click into place Be sure to make note of the test results or print for e
35. re 19 Micro Cuvette Holder Right side View Note Light beam guide is eliminated from latest design change Peltier Flowcell Accessory Installation Guide S 2100 109P Step 1 Check all items from the accessory package and make sure they are complete refer to Figure 14 for S 2100 109P Peltier Flowcell Controller with peristaltic pump 1 unit Cuvette Holder with Peltier base and Flow thru Panel with tubing 1 set Installation Guide 1 pc flowcell is required and flowcell is not included in S 2100 109P package Installation preparation and installation procedures tool needed standard Philips head screwdriver Step 2 Remove the 4 position Cuvette Holder from the Sample Compartment Open the Sample Compartment cover and remove any cuvettes or any breakable items Push in the Cuvette Holder Control Knob black knob all the way back First position Cuvette Holder into light beam Step 3 Unscrew the Cuvette Holder rod by turning the knob counterclockwise until removed from holder Gently slide the guide rod out of the sample compartment Step 4 Looking over the Sample Compartment remove the front screw Figure 15 Step 5 To remove the back screw place your hand ON TOP of the 4 position Cuvette Holder and gently slide towards the front The back screw is then exposed and can be removed Step 6 Remove the front panel by sliding the panel up and out Figure 16 Step 7 Insert Cuvette Holder with Peltier Base into the Sample Compart
36. the front The back screw is then exposed and can be removed Step 7 Insert Universal Holder by repeating Step 4 to 6 in reverses Step 8 Screw in the Water Jacketed Holder to the first front position of the Universal Holder platform This is to prevent stretching the tubing Step 9 Remove the front panel by sliding the panel up and out Figure 16 Removable Front Panel Front View IT Figure 16 Removable Front Panel Front View Step 10 Insert Front Panel for Water Jacketed Cuvette in place of removed panel 27 Step 11 Step 12 Connect Water bath to the front panel tubing connectors Figure 17 Front Panel for WaterJacketed Cuvette Accessory Tubing Connectors Figure 17 Front Panel for Water Jacketed Cuvette Accessory Set desired temperature on water bath see instructions of water bath manufacturer Micro Cuvette Holder Package Accessory Installation Guide for S 2100 106P Step 1 Step 2 Step 3 Step 4 Step 5 Step 6 Step 7 Remove all items from the accessory package made sure that they are complete refer to Figure 14 for the S 2100 106P A Cuvette Holder with X Y adjustable bas 1 pc Installation Guide 1 pc The only tool needed is a standard Philips head screwdriver Remove the 4 position Cuvette Holder Open the Sample Compartment cover and remove any cuvettes or any breakable items Push in the Cuvette Holder Control Knob all the way back First position Cuvette Holder is
37. the standard package e Power Indicator Indicate whether power is on or off e Cuvette Holder Control Knob Select the sample position of the 4 position Cuvette Holder by pulling out or pushing in e Operation Panel has eleven components see below for details 6 Figure 4 illustrated the detailed information of the Operation Panel 1 Data Display Screen Display the data of Transmittance Absorbance Concentration and Factor modes Mode Indicator The red LED light indicates the operation MODE Allow the operator to know the measurement mode currently in use T Transmittance A Absorbance C Concentration and F Factor 0A 100 T Button Blank the 2100 2100UV when blank reference solution is in the Sample Compartment Every time a new wavelength is selected press 0A 100 T Button to confirm It will automatically blank the 2100 2100UV 4 MODE Button There are four modes T mode is transmittance mode A mode is 10 11 absorbance mode C mode is to measure unknown sample concentration through a standard solution F mode is to measure unknown value with a previously determined factor PRINT Button Press to send Data Display Screen and Wavelength Display Screen results to RS 232C port and printer Wavelength Display Screen Display the current desired wavelength Wavelength Control Buttons Allow user to select the desired wavelength with the and V increase and decrease buttons INC DEC Concentration Fact
38. ure change properly installed or has Lamp not adjusted properly moved during transit Refer to lamp replacement instructions in this manual Lamp old or defective Replace with a new lamp Sample Holder Misaligned Refer to lamp replacement instructions in this manual Unstable power supply Call an authorized service Defective or dirty detector or defective engineer electronic component Incorrect Insufficient sample volume Fill cuvette with more readings samples obtained Wrong wavelength setting Check analytical procedure Failed to blank 0A 100 T Failed to set O T and wavelength setting Check wavelength accuracy according to procedure in this manual Stray sample preparation vapors Prepare sample away from instrument Use proper ventilation Bubbles or particles in solution Check sample preparation and analytical procedure Instrument out of electronic calibration Call an authorized service engineer 24 Error Codes The Error Codes are the classification for errors detected automatically by the instrument Each code represents different errors that occur during the self calibration or during operation The Error Codes are displayed on the Wavelength Display Screen and are defined as Table 5 Table 6 Error Codes Error Code Definition Function Causes Solutions ERR 01 Instrument unable to initialize Check power supply to see if proper voltage the lamp 2100UV or secondary is selected
39. velength at 340 nm insert an empty cuvette and blank the instrument If the energy is low adjust the lamp by pulling or pushing it so that the light beam is focused on the entrance slot of the monochromator Since the lamp socket is pre aligned there will be minimum if any adjustment required 7 Reinstall the instrument cover by positioning the front of the cover first and then sliding the back of the cover over the backside grill plate Be sure all wires from the front panel are not pinched in the process Note The latest version of Model 2100 2100UV has a lamp access door at the back of the instrument If your Model 2100 2100UV has the lamp access door you do not need to open the cover Just open this door to replace the lamp 8 Reinstall the four screws and the Cuvette Holder Control Knob B Replace Deuterium Lamp WARINING Wear UV protection Glasses before changing the Deuterium Lamp Turn off and unplug the instrument VERY IMPORTANT HIGH VOLTAGE Remove the four screws on the sides of the spectrophotometer Remove the Cuvette Holder Control Knob by unscrewing the rod counterclockwise Remove the cover of the instrument very carefully and place it in front of the instrument ED he Saal HINT Lift up about 3 4 inches and once the instrument has cleared the backside grill plate lift the back of the cover up towards the front The front of the instrument will act as a hinge BE SURE TO NOT PULL PANEL WIRING
40. window 0 48 ml S 90 346FQ Flowcell Quartz 4x12 mm window 0 48 ml S 90 350G Square cuvettes optical Glass 4 mm width 1 0 ml S 90 351Q Square cuvettes Quartz 4 mm width 1 0 ml S 90 352Q Square cuvettes Quartz 4 mm width with stopper 0 5ml S 90 353G Square cuvettes Optical glass 2 mm width 0 5 ml S 90 354Q Square cuvettes Quartz 2 mm width 0 5 ml S 90 355Q Square cuvettes Quartz 2 mm width with stopper 0 5 ml S 90 358Q Square cuvettes Quartz 2x5 mm window 0 100 ml S 90 359Q Square cuvettes Quartz 2x2 5 mm window 0 0 50 ml Misc and Repla cement ltems S 1100 508 Printer Paper Package of 3 S 2100 510 User Manual S 2100 511 Dust Cover S 2100 515 Tungsten Halogen lamp for 2100 and 2100UV Package of 2 6V 10W G4 type S 2100 525 Deuterium lamp for 2100UV S 2100 535 Fuse 3A quantity 1 size 5 x 20 22 Troubleshooting Table 5 Troubleshooting PROBLEM Possible Cause Solution Instrument Power cord not connected to outlet Plug instrument in Inoperative Dead Power outlet Change to a different outlet Power Wrong voltage setting indicator has no internal fuse blown or defective Call an authorized service light electronic component engineer Instrument No cuvette in the Sample Compartment Cuvette adapter must be in cannot set Sample Compartment to 100 T 0 000A open sample holder shutter Light beam blocked e Holder misaligned e Shutter Lamp is old
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