SimpliNano Quick Start Guide
Contents
1. SimpliNano Quick Start Guide Product Numbers 29061711 29061712 This guide provides a summary of the procedure for using the SimpliNano spectrophotometer from GE Healthcare Life Sciences It is meant to be used only after a User has been fully trained according to the system s User Manual and the work site procedures This guide assumes that the User is fully aware of the health and safety issues associated with this system and its components as described in the system s Health and Safety Manual and User Manual Legal GE imagination at work and GE Monogram are trademarks of General Electric Company SimpliNano is a trademark of GE Healthcare companies All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them A copy of these terms and conditions is available on request 2013 General Electric Company All rights reserved First published October 2013 http www gelifesciences com SimpliNano measuring Nucleic Acids and Proteins SimpliNano can be used 2 Select application mode to measure the following approximate concentrations and volumes a Select Application SimpliNano DNA 5 2500 ng l mode of choice e e Protein 0 2 50 mg ml DNA ANA Press 1 for DNA o P ress 2 fo r R N A Absorbance Protein 4280 Concentration 1 Power up the Press 3 for Oligonucleotide of instrument Press 5 for Protein Uties Volumes betw2. een 1 5 ul 3 Review default settings for method DNA Parameters The default settings are recommended for routine Units 08mm measurements ugiml Dilution Factor For example the DNA parameters screen will allow a dilution factor to be entered for automatic calculations the concentration factor and units can be changed and the background subtraction may be turned off 4 Take Reference sample 5 Measure sample Pipette 2 uL of reference solution Pipette 2 uL of sample for example water or TE buffer into into the indicated the indicated sampling port sampling port Press the button to read Press ay to read When measurement is complete clean When measurement the sample port with a lint free tissue is complete clean the Once reference information is stored sample port with a multiple samples can be measured lint free tissue 6 Results a A230 0 089 4 Parameters The results will be displayed on the screen can noes as shown for DNA A280 0167A Graph If you have the optional printer attachment j press 53 to display available Options and 154 press 2 to Print Units Save Method 2 90 ug ml Auto Print imagination at work 29088791 AA 10 2013
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