Abacus junior
Contents
1. Abacus junior User s Manual 6 1 3 Instrument features Figures 1 and 2 show a front and a back view of Abacus junior hematology analyzer SOOO SE OD desee Tus cAbacus junior Diatron Figure 1 Front view Floppy disk drive OK key Numerical keypad Function keys Graphic liquid crystal display HELP key Measure function key Built in printer Database function key Utilities menu key Printing function key Exit menu key Cursor control keys Status indicator START key Sample rotor with interchangeable adapters Abacus junior User s Manual pori Figure 2 Rear view Reagent tubing connections On Off switch External power supply inlet 12VDC PS2 external keyboard port Serial RS 232 port 1 Parallel printer port Abacus junior User s Manual 8 Figure 3 shows an open built in printer using roll paper inside To open the lid press the black button Just put in the thermal printer paper and close the lid so that the end of the paper should be clipped between the black paper guide and the printer mechanics You can select to use this printer in the Printer Settings menu Paper guide Thermal paper Printer mechanics inside y Lid opener button Figure 3 Built in printer The analyzer works with an external power supply The next figure shows the power supply unit generating 12VDC The power supply modules have an auto range input which makes possible to use them with2. Table 5 Calibration ranges PLT Quality Control After entering the required reference values BOB 083 18 9 perform analyses on the control material MPv TA LM 3 20 2 mr PCT G2 MID 0 04 Measure Infa IPDWc 38 6 GRA 4 38 Press to accept results RPrv 331 332 Lyse G78 ml WPrVI 333 336 The number of calibration analyses performed is shown on the first line Following calibration the new factors are displayed The previously used factors are shown in parentheses for reference Offered calibration factors can be accepted by pressing LW The factors will not be modified but are flagged If0 was entered for low and high limits Bifblank was not in the acceptable range E if the factor is out of the 0 80 1 20 range o CO gt 0 1 1 5 1 4 3 View calibrations From the Calibration menu you can monitor previous calibration factors The instrument logs all calibration events and displays them in the following format 11 06 2003 13 29 10 06 2003 11 31 16 05 2003 Herd 12 05 2003 19 47 12 05 2003 HT 09 05 2003 14 54 09 05 2003 14 46 Hz 2H 2105 Abacus junior User s Manual 32 5 1 4 4 Calibration settings When CALIBRATION SETTINGS is selected the following screen appears Calibration settings MEASURE DRR Calibrate MCY and MPY CALIBRATION 3 Oz Calibrate HCT and FCT CALIBRATION SETTINGS amp 3 Factorial calibration 3 O4 Calibrate with ane meas
3. 15 85 2883 02 44P Pressing the lt or key accesses the 16 85 2003 08 376 remaining non visible parameter results The 26 05 2003 12 30P T or y key scrolls between the results 05 06 2003 10 56 individually The 3 and 9 keys are equivalent 18 85 2863 03 29F to PageUp and PageDown scrolling keys Select patient results by pressing the OK key The box will be filled From the database table screen WBC RBC and PLT histograms can be displayed by pressing EL By pressing the 9 key the second panel with PLT results can be viewed Browsing among selected tests can be made by pressing DOWN and UP ARROW function keys 1 and Databaselocalmenu e DATABASE LOCAL MENU iB o to specified record Selection 3 Change sort order From the database table screen enter the 4 Manage selected records Database local menu by pressing the 5 View external key b Backup one dau DATABASE LOCAL MENU bo to the first record measured after GO TO SPECIFIED RECORD 1 10 06 2003 Bid Item 1 of the previous menu accesses a and has Sample ID screen asking for parameters defining any given sample date time ID and jumps to it If any ID is left as 0 searching is performed only by date time and has Patient IDs Abacus junior User s Manual 42 j lt DATABASE LOCAL MENU gt iB Select by date time and ID SELECTION 2 2 Select all Besides checking samples one by one 3 Deselect all selection can also be done within
4. Abacus junior User s Manual 1 2 2 Patient Testing The analyzer can process 30 blood samples per hour Samples can have individual sample data and additional parameters Full histograms of the blood cells are also stored along with the above data Results can be printed on the optional internal or external printer The print out format can be customized by the user Abacus junior determines 18 hematology parameters including three part WBC differential The instrument requires 25 ul of the whole blood sample WBC total white blood cell count LYM lymphocytes count MID monocytes eosinophils count GRA granulocytes count LY lymphocyte percentage MI monocytes eosinophils percentage GR granulocytes percentage RBC red blood cell count HGB hemoglobin HCT hematocrit MCV mean corpuscular volume MCH mean corpuscular hemoglobin MCHC mean corpuscular hemoglobin concentration RDWc red cell distribution width PLT platelet count PCT platelet percentage MPV mean platelet volume PDWc platelet distribution width NOTE If the equipment is used in a manner not specified by the manufacturer the protection provided by the equipment may be impaired Misuse of equipment or use other than its intended purpose will invalidate conditions of warranty The accuracy and precision may also be impaired Abacus junior User s Manual 5 1 2 3 Reagents Only reagents supplied by DIATRON should be used with the analyzer other
5. Blood cell suspension RN M Baa eo 5 External electrode ABB Figure 6 Impedance method Each cell passing through the aperture where a constant DC current flows between the external and internal electrodes causes some change in the impedance of the conductive blood cell suspension These changes are recorded as increases in the voltage between the electrodes The number of pulses is proportional to the number of particles The intensity of each pulse is proportional to the volume of that particle The volume distribution diagrams of the particles are WBC RBC and PLT histograms Pulses are counted only in channels in terms of femtoliter fl which are between the lower and upper discriminators 4 2 Principle of HGB Measurement The lysed 1 196 sample dilution can be measured by a cyanmethemoglobin method The reagent lyses the red blood cells which release hemoglobin Hemoglobin iron is converted from the ferrous Fe to the ferric Fe state to form methemoglobin which combines with potassium cyanide KCN to produce the stable cyanmethemoglobin or hemoglobincyanide Subsequently the HGB concentration is measured photometrically Note The above mentioned measuring method is used to determine the HGB concentration The HGB concentration can be measured using cyan free lysing reagents as well In this case the effect is the same but the used lyse is environmental friendly reagent Abacus junior User s Man
6. ad Menu SUCUT Cd decias 23 4 OPERATING PRINCIPLES cis iaa 24 4 1 IMPEDANCE MET OD a A E SUDDEN REN datado ocacion DUE AIES 24 4 2 PRINCIPLE OF HGB MEASUREMENT ssp 24 4 3 PARANEE a E E A E E E DEDI DIM DE ED ESUS 25 4 4 ABSOLUTE AND LINEARITY RANGES OF MEASURED PARAMETERS sccecceceecescecceccecescccssctcescscucens 26 5 ROUTINE UTILIZATION AND MEASURE ii osooso oeoo noooooosoooooooooosoo oono 27 5 1 NMENXSURINGIEROCES oeei E E E E dc 27 Dd Sample handling ME 2 Sd EA A A 28 SN E IA A 28 NE EEG 07 0D ee eT Te ee ene ee eee ene ee ee eee ere ee ee eee eee 29 Tdo AMM ING VSI iia PL URL DEL o es 32 Id Mn A E nn A 36 DAD Prediluted A 38 TS NEL nee 39 6 DATABASE C 41 7 Abacus junior User s Manual 2 452 7 3 7 4 7 5 7 6 8 PRINTING 8 1 PES A E A E ENEE E E T E E A R 44 A ME A In E OAA 44 VE D A AAA E E E E Ue o O E I S ENEE EE OEE 45 LaS WEI ao A E O E EE 45 lo teile tall 47 Lad O AN E E E NE 47 A A E 48 PRINTER SH E RR RETE 49 7 3 1 Troubleshooting guide for printing problems oooooonnnccnnninnnnnnnnncnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnananinnss 52 die 53 Pad MICO T eae m 53 Pa SAS 54 LA Fld enS OVS A 54 LA Poma e e A E EA EE E AA E E EEE E 55 TES AFO AE Gaining E E
7. 230V or 115V mains outlet and it has the CE and UL safety certification The input socket is a standard power cable connection the output is a special lockable socket as it is shown in the picture Figure 4 External power supply unit Abacus junior User s Manual 9 1 4 Parts of the Analyzer Abacus junior hematology analyzer is composed of three main units Fluidic System Performs sampling diluting mixing and lysing functions Generates the regulated vacuum used for moving cells through the aperture during the counting process Data Processing System Counts measures and calculates blood parameters generates and stores numerical results and histograms Control Panel Features an LCD display a 29 button keypad and parallel external printer and serial computer interfaces 1 4 1 Function of the Fluidics For the Schematics of the fluidics system see Section 9 5 Sample aspiration and dilution Sample procedure 25 ul 50 ul in predilution mode of anti coagulated EDTA whole blood sample is aspirated through the sampling needle mixed with 4 ml of diluent and stored in the chamber primary dilution 25 ul of the primary dilution is aspirated into and stored in the needle during WBC measurement and the hemoglobin analysis Lysing reagent is added to the primary dilution held in the chamber for WBC differential analysis This amount of lysing reagent is species dependent and may be changed by the operator
8. learning instrument operation c Pressing the numeric key corresponding to the desired menu item allows selection and validation of an item without the need to additionally press the OK key Pressing the 0 zero key has the same effect as the LE function key This method is more efficient after the user knows the menu structure d You can also move between the different menu levels using the lt and gt keys These have the same effect as OK and LL respectively If selection of a menu item would open up a submenu then that item is indicated with a gt symbol at the end of the menu line Abacus junior User s Manual 22 Some results can be displayed in table format The following keys may be used for browsing the database 31 page up 91 page down 11 jump to top of list rans jump to bottom of list Several menus have items with boxes in front of the text These indicate two state options The selected state is indicated with a filled box the deselected state is indicated with an empty box Selecting the item toggles its state Other items have circles in front of the text These are called radio buttons They are divided into groups separated by horizontal lines The function of these groups is that only one item of the group can be selected and this is indicated with a filled circle in front of the selected item Selecting an item of the group will move the filled circle in front of this item emptying the circle
9. local menu 5 1 3 Blank measurement Blank measurement is used for checking the cleanliness of the system and the reagents Blank measurement must be performed e Once daily before sample analysis this is done automatically before the first analysis in the MEASURE e After any reagent change activated manually from the MEASURE MEASURE BLANK menu e After the replacement of any hardware component that is closely related to the measuring process aspiration dilution counting rinsing MICH vo FBC MCHC PLT Lys WEC Blank measurement The user must accept blank values by 14 06 2003 18388 pressing LW d To repeat the blank measurement press Hrs bk If any of the tested parameters has a AT a high blank value the message SEUL Lass a Unsuccessful blank measure appears at the top of the screen Abacus junior User s Manual 29 There are 3 regions for blank value handling 1 Optimal all results are within acceptable ranges 2 Blank is high flag is displayed at relevant results 3 Blank exceeds acceptability no results displayed Parameter 1 No flag at parameter 2 flag at result 3 E error flag at result WBC PLT RBC l 0 25 x10 cells ul 25 50 x10 cells ul 50 x10 cells ul O 0 05 x10 cells ul 0 05 0 5 x10 cells ul gt 0 5 x10 cells ul Table 4 Blank measurement ranges Accepted blank values are essential for proper calibration and quality con
10. making reports on measured samples 8 1 Printouts When required the following items can be sent to an external printer or to a built in printer by pressing the function key button e Database result s table format Database specified patient results with histograms QC result Levey Jennings chart QC result s table format Last measured blank result Last measured patient result with histograms Last measured QC result Device information and statistics Self test result Set parameters Printout formats of typical patient results are shown in the next figures the appropriate printout format can be selected in UTILITIES PRINTER SETTINGS Abacus junior User s Manual Full printout format with histograms Patient info Sample and measurement info Header sampla De 18 Test dates 2nig2 11 15 Test times 16 23 Doctor Report date B03H2 87 Hama Birih Sex r amne me ng node NBC 7 329 i69 LTT Log ies nip AL 189 1 GRA 450 1831 E OS SA ee a o sammo ee ee Locatiof of warning flags Limits or graphical location of the not displayed now result within the range of limits 60 Histograms oo 408 In this printout reference ranges limits in graphical form flags and warning flags are enabled The printing modes of these parameters can be selected within PRINTER SETTINGS submenu The normal range of each parameter is indicated with a rectangle The left side of the rectangle show
11. of the old selected item Abacus junior User s Manual 3 1 2 Menu Structure if multi user mode is enabled if serial communication is enabled F8 local Repeat last sample Measure blank F9 local Go to specified record Selection Change sort order Manage selected records Viewexternal gt Back up one day Utilities F10 Printer settings Settings Empty waste container Service F12 Shut down Logout Prepare for shipment Calibrate View calibrations Calibration settings Calibrate MCV and MPV Set QC reference Toddler Child Select by date time and ID Select all Deselect all Sort by time Send selected records Delete selected records Backup selected records Drain chamber Statistics Self test Calibrate HCT and PCT Factorial calibration Calibration with one measure Calibration with three measures Calibrate prediluted mode QC level 3 Diluent amp cleaner l Lyse Calibrate fluid sensors Single user mode Multi user mode 23 Abacus junior User s Manual 24 4 OPERATING PRINCIPLES 4 1 Impedance Method The impedance method a k a Coulter method counts and sizes cells by detecting and measuring changes in electrical impedance when a particle in a conductive liquid passes through a small aperture M DEDE a 1 Internal electrode E E Me Y Y o A s m 0 7 L Aperture NEN j E 1
12. short cut keys for easier navigation between menus e Cursor control keys and 4 for moving between database items lt and gt for moving between parameter columns or menu levels e START key for initiating an analysis cycle e OK key for confirming data e Del key for deleting characters e Help key for HELP function Abacus junior User s Manual 11 Function keys Below you can find all the possible icons and functions assigned to the so called soft keys function keys Function Action triggered Exit from actual menu or action Leave data entry menu without saving any changes made to it aaa m tas Bene ia lad patri OCA Redo action e g Blank ee Ga pito outs potent 0 Gc c Select between result pages _ PAGE UP key in a multi page menu PAGE DOWN key in a multi page menu Change scaling of Levey Jennings chart 16 or 64 days Patient type selection Go to local menu database measurement Below you can find all the possible icons and functions assigned to the so called hardware function buttons Function Action triggered key Measuring process at once Abacus junior User s Manual 12 1 5 Control Material Abacus junior enables continuous monitoring of six different Control Materials control blood These should be matched to the types of samples usually run on the instrument Specification assay values and allowed tolerances along with expiry date regarding these materi
13. within Limits menu PM WBC counting and a washing process 5 ml of diluent is added to the second dilution using the 25 ul of primary dilution stored in the needle This portion is analyzed for RBC count PLT count and their parameters Table 1 Dilution rates used within Abacus junior e Primary dilution 1 160 e RBC dilution 1 32 000 WBC dilution 1 196 dependent on lyse amount Measurement times e WBC count 8 seconds e HGB measurement 3 seconds e RBC PLT count 8 seconds Abacus junior User s Manual 10 1 4 2 Control Panels START button Pressing and releasing the sampling bar triggers an analysis cycle Status indicator A three color LED is located above the START button Its actual color indicates the status of the analyzer LED color Analyzer status e Green The analyzer is ready to work Analysis can be initiated The analyzer is currently performing an analysis No new measurement can be started Yellow The analyzer is performing a maintenance process or is in stand by 1 4 3 Display The display is a 240 x 128 dots high contrast CCFL backlit graphic LCD Liquid Crystal Display module 1 4 4 Keyboard The foil keypad is composed of the following shown in Figure 1 e Numeric keys for entering numerical data and selecting menu items e Function keys having specific functions These functions are menu dependent and are indicated by icons appearing above the keys e Hardware function keys
14. Abacus junior Hematology Analyzer User s Manual 1 1 release Dialron Abacus junior User s Manual 1 TABLE OF CONTENTS 1 INTRODUCTION nui 3 1 1 INTENDE PUSO tac bi 3 1 2 GENERAL DESCRIPTION cotarro rica 3 5T EE A A A 3 A TA aterert dui ann eeesanne sap cpi Sa eaten 4 JN ME A m 5 L2 TOCCATA O sosticisenincsit rein o outage oa rex ta E a 5 L23 SCOMDTAMOM GHG Onal CONTO Ladder E as 5 1 3 INSTRUMENT FEATURE ne ee er ere mT ere ae Pre eres 6 1 4 SO THE ANALYZER MT 9 D EE UE OEO he QU Te icenen e e T E ot T OE 9 A CoO ag 6 1 S ton Ce a OEE PREC E PSL eT EI E MU DIIM ET 10 DX EP n Rn eRe Ee eee E A ee eon ne ee E A eg 10 DU E An eee AT An ee 10 1 5 CONTROL MATERIA doo 12 1 6 A ocasi n sabias 12 1 7 SEAN a E EE ee ee 13 2 RE PELO a E 15 2 1 GENERAL INFORMATION cccccssecoscccsccscccscccsccssccsscescesscosscescescccsscescesscesscescsssccsscescesccesssescesccesseescesceess 15 2 2 ENVIRONMENTAL FACTORS i n 15 2 3 UNPACKING AND INSTALLATION diia 16 23d Turine the Instrument ON m 18 2 3 2 Turning the Instrument OFF SEM viiccccccccccccssccccccccccessesececceeeeaaeeseeceeeeeaaaeeseeceeeesaaeeeeeceseesaaaneeees 19 2 3 3 Preparing for SAIPMENE cccccccccccsaneeeccccceceea a eeseeeeeeeeaeeeeeeeeeeeeaaaeneeeeeeeeeaaaaeeseeeeeseaaaasseeeeeeeeaaaasses 20 3 MENU SYSTE A 21 3 1 GENERALE JNBORN S TON Ctra ica 21 Jd o INGUEISQUARE m ihe Men ys tea 21
15. BC blank again Possible lyse contamination or noise problem C Q WBC clogging Aperture clogging Perform cleaning and repeat the measurement If it is a general problem please contact your Service Personnel Low temperature reagents can cause it as well mainly diluent in this case you will have to wait until they reach room temperature Table 7 Summary of warning flags related to WBC HGB Warning flags in lowercase refer to RBC or PLT problems Flag Meaning Recommended user action PLT blank is high or no Run cleaning and repeat the blank measurement PLT blank Diluent or system cleanliness problem If it is stable high replace the diluent by opening a new tank RBC blank is high or no Same action as in case of warning fla RBC blank o RBC PLT clogging The same action as in case of the C warning flag Table 8 Summary of warning flags related to RBC PLT Warning flags can be grouped according to measurement conditions and according to the problems relating to the blood sample Measurement conditions when the flags are related to clogging c C probably hemolysing problems E b B p and pressure problems Fatal pressure error In this case we suggest repeating the measurement The asterisk flag near a parameter shows some doubt suspected during the evaluation of that parameter The reasons can be a high PLT blank PLT value will be marked a case of indefinite discriminator setting d
16. Database local menu SELECT ALL checks all boxes DESELECT ALL clears all boxes Select specified data records DATABASE LOCAL MENU select data records measured between SELECTION 2 0 06 2003 BABA 10 06 2003 11 59P SELECT BY DATE TIME AND ID and has Sample ID in range 1 I Select a range by date time and or ID and has Patient ID in range number see next screen Entering O as an ID results in a search by x l e ldate only Corresponding results will be marked with a filled box Changesortorde A DATABASE LOCAL MENU C11 BERI T CHANGE SORT ORDER 3 me Sort bu time 3 Sort bu sample ID Od Sort by patient ID The order of results can be selected within this menu DATABASE LOCAL MENU iB Send selected records MANAGE SELECTED RECORDS 4 2 Delete selected records From this sub menu you can send the 3 Backup selected records selected database to a PC or you can delete them or you can save on a floppy and disk Data backup and monitoring are accomplished in this submenu Before menu selection insert a 3 5 floppy disk into the drive located on the front bottom panel of the analyzer An empty floppy disk can store 800 samples Eaei CT DATABASE BACKUP ONE DAY 6 Specify the day whose records you wish to Day to backup backup to a floppy disk 11 06 2003 Abacus junior User s Manual 43 When you have selected the day or data to be IM 104 1221 eee ndi saved and co
17. E E SA 55 SORIA RE UPDATE RM 58 FLUIDIC SCHEMATICS NORHRRCRT 58 A E 59 PEO OUT AAA AE EEEE AEA E OEE AE E E EE E EAA 59 Abacus junior User s Manual 3 1 INTRODUCTION 1 1 Intended Use Abacus junior hematology analyzer is a fully automated cell counter designed for in vitro diagnostic use The instrument was developed for use in hospitals and small to medium sized labs 1 2 General Description 1 2 1 The Instrument Abacus junior is a fully automated benchtop hematology cell counter It implements the so called Coulter method for counting cells passing through a small aperture and measures the hemoglobin content of red blood cells The analyzer features a graphical LCD display module and a foil keypad of 24 keys including 6 software buttons with icons 6 hardware function buttons above LCD and has a START button The instrument allows sending results to an external printer parallel port or can have an optional built in printer module Its internal memory is capable of storing 1000 records with full histograms and individual patient data QC measurements can also be performed and stored The software operating the instrument is easy to upgrade using a standard 3 5 floppy diskette or CD ROM optional The instrument allows connecting to a host computer for uploading records stored in the memory through RS 232 serial port and also enables archiving and restoring records to and from floppy diskettes
18. als is always enclosed with the approved control material 1 6 Accessories Below you can find the list of accessories shipped with your Abacus junior instrument This list can also be referred as the DIATRON Abacus junior pack Abacus junior Veterinary Hematology Analyzer Abacus junior User s Manual this booklet Abacus junior Reagent Tubes marked with colored connector caps Diluent tube green Lyse tube yellow Clean tube blue Waste tube red Abacus junior Cleaning Tube Kit Abacus junior Caps for reagent containers marked matching reagent tube colors Abacus junior Power cable and external power supply Abacus junior Spare Part Pump tube Abacus junior User s Manual 1 7 Specifications Sample volume Aperture diameter Throughput Characteristics WBC RBC HCT MCV HGB PLT Sampling method Sample types Fault statistics Clog prevention Cleaning procedure Quality control Calibration Multi user feature User interface Languages available Data storing capacity Host computer interface Data back up Interface Software upgrade Printer interface Optional built in printer Display Keypad External keyboard Power supply Power supply Input 13 25 ul whole blood 50 ul in Prediluted mode 80 um in one universal measuring chamber approximately 30 tests hour Accuracy Reproducibility Carry over Test range max deviance between from expected CV samp
19. arameters as well as the WBC RBC and PLT histograms Results and histograms will be stored automatically in the memory without any EL Bos MEA 25 97 operator confirmation RBC 5 84 MCHC 328 4145 PLT 272 f reference ranges are set not 0 0 HET 44 3 L Y 25 8 parameters will be verified and marked by ev SSH 18 if the value is over RDWc 15 1 6Rx 63 4 if the value is under the range specified STE 3395 c e f analysis errors occur or the blank measurement is too high the E error flag will appear at the erroneous parameter and no results will be displayed for it instead will appear e f there are warnings a flag will appear preceding the result In the last line of the first result screen warning flags can be displayed Abacus junior User s Manual 35 In the followings we summarize the warning flags and give an explanation of their possible cause and a few hints to overcome the problem Uppercase letters refer to WBC or HGB problems Flag Meaning Recommended user action No WBC 3 part differential Possible lyse problem May occur in pathological lymphocytosis HGB blank is high or no Repeat the blank measurement If HGB blank is not stable HGB blank there are probably bubbles in the WBC chamber Run a cleaning and try blank again Close the side door if open during measurement WBC blank is high or no Repeat the blank measurement or run prime lyse and try blank W
20. d samples In the Prediluted mode the operator must prepare an external 1 3 predilution as in the following example Predilution 7 UNIT OF SAMPLE 3 UNITS OF DILUENT Example if 20ul capillary tubes are used for blood collection add 60ul of pure diluent to create a proper predilution Measure local menu The Prediluted mode must be selected in the repeat last sample MEASURE 2 Measure blank 3 Calibration 4 Quality control 05 Prediluted mode Bb Limits 1 3 sign will appear on the WBC histogram of the result screen NOTE The instrument is capable of measuring two kinds of prediluted mode 1 3 and 1 10 The user cannot change these modes Contact service for further information The results are corrected with the predilution factor 1 3 automatically NOTE The Prediluted mode has its own calibration factors To calibrate the instrument for this mode see the Calibration settings section of this manual To disable the Prediluted mode re enter Measure local menu and deselect its box Abacus junior User s Manual 39 5 1 8 Limits This function allows the user to specify the patient limits or reference ranges used in your laboratory Lower and upper limits for each parameter are displayed and can be modified The quantity of lyse is also set in this sub menu Five different patient types can be accessed MEASURE LIMITS 6 Female 3 Baby In this menu you can set the limits of the 4 Toddler diff
21. e password corresponding to the ID a user with supervisor rights is required for changing or re enabling the users password for the instrument There MUST be always a user with supervisor rights Would the user with supervisor rights forget the password for his her ID only an authorized service person can re enable entry of the supervisor and change the password The multi user mode permits the identification of operators through their personal settings or profiles It also restricts users to certain software functions In Abacus junior the term multi user means storing profiles for different users but does not mean allowing more than one user being logged in simultaneously e An operator at Basic level can perform analyses and enter patient data prior to the measurement process Abacus junior User s Manual 56 e Advanced users in addition to Basic level functions can modify instrument software settings perform calibration and quality control and modify patient data when browsing the database e A Supervisor has the ability to do all of the above and to modify user access or passwords With multi user mode enabled users with different access levels will have different abilities within the menu system Some items will not be accessible for them USERS 5 LIB Single user made bom 7 This menu allows configuring the instrument UM CE for single user or multi user mode Care must be taken when changing to multi
22. e instrument before connecting the external power supply to the instrument and to the AC outlet as well as before connecting an external printer or a keyboard to the instrument Abacus junior User s Manual 17 7 Reagent Containers Place the reagent containers near the instrument to an accessible location Do not place the containers to a higher position than that of Abacus junior because if the tube comes off the connector the fluids will spoil out Use the supplied connecting tubes and special bottle caps Be sure that the color on each tube cap and connector in the back of the instrument match You can for example place the reagent containers below the desk Abacus junior is installed on as the instrument has sufficient power to draw the liquids from a lower location All containers should be left open do not block the small air vent hole on the special container caps in order to provide free airflow For connections see Figure 5 Diluent Diluent solution Waste container c c o OQ O 25 3 o O uN uN Figure 5 Reagent connections Abacus junior User s Manual 18 2 3 1 Turning the Instrument ON a In case you use an external printer for information read manual shipped with the printer connect it and turn it on b Turn on the instrument by flipping the power switch above the external power inlet to position An automatic start up procedure will be initiated During start up the following screen is displa
23. ectronic pulses cleaner solution and high pressure backflush 7 1 3 Priming During the priming cycle the fluidics system is rinsed with a large amount of diluent It differs from the process in a start up procedure as in the latter case a simple filling up of the fluidics is performed If fluid sensors are on then the analyzer makes these procedures automatically otherwise the User must initiate them activating the appropriate item within this submenu Abacus junior User s Manual 45 7 1 4 Draining chamber Draining of chamber should be run before removal or replacements of parts related to the measuring chamber or aperture 7 1 5 Weekly maintenance Weekly maintenance should be performed before turning on the power switch 7 1 5 1 Cleaning the washing head The washing head cleans the outer surface of the aspirating tip with saline diluent Any salt build up on the lower surface may cause malfunction during operation Use warm tap water and a soft cloth to clean this area You can see the washing head indicated in the following figure Washing head Measuring chamber Measuring aperture Figure 9 Parts of measuring block 1 Exit the Measurement menu Open the side door after the needle has stopped moving 2 Gently rub the lower surface of the washing head to remove the salt build up 3 Close the side door Abacus junior User s Manual 46 7 1 5 2 Peristaltic pump maintenance The pump installed in the instr
24. efault location is used for some reasons related parameters will be marked etc Another flagging method is evaluation against the normal ranges If some of the parameters is out of range it gets a flag if under the range or gets if over the range You can customize ranges for all kind of patients by setting the corresponding lower and upper ranges If you set O for a range limit it will be not verified Abacus junior User s Manual 36 5 1 6 Quality Control By analyzing control materials day to day reproducibility can be monitored In this submenu both target values and acceptable ranges for each parameter can be specified for different QC levels The Abacus junior provides six different Quality Control levels You can set up six individual reference sheets for each control material e g low normal and high control blood QC measurement results will be added to the selected level as indicated at the top right corner NOTE Target values of the control material should be set only once at the beginning of the QC measurements Resetting parameters deletes previous QC results of the active level CAUTION Any change in the QC material setting deletes previous QC results It is strongly recommended to print results prior to changes Quality control From the MEASURE you can access Quality 1 STRUM reference Control Menu 2 QC measure MEASURE 3 View table of GC measures QUALITY CONTROL 4 4 View OC diagram This m
25. enu allows entry and monitoring of data 5 Set QC level p related to the QC procedure Actual UC Level 1 Use commercially available control material The preparation and analysis process is the same as with patient samples Sete ce MEASURE amp 1 OC Level 1 QUALITY CONTROL 4 SET QC LEVEL 5 Select the level you wish to use The active level is displayed in the top right corner on QC related screens MEASURE QUALITY CONTROL 4 SET QC REFERENCE 1 05 05 2003 Both target values and acceptable ranges can 7 50 0 50 169 1 be specified Only the parameters displayed on 4 50 6 15 10 12 these screens are utilized Modify displayed 132 4 gil values using the numerical keyboard Pressing _ Il the OK key accepts data Use the page down function key to view additional parameters Setting 0 0 disables QC of the parameter Abacus junior User s Manual 37 NOTE Quality control measurements can only be made after an optimal blank measurement result has been accepted all parameters were in the 1 range PLT Ouality Control MEASURE QUALITY CONTROL 4 Po E QC MEASURE 2 MESS mm PDH 38 6 GRA 4 30 After selecting target values or targeted RPrV 331 332 Lyse 8 78 mi level use the above menu to perform a QC Wry 333 336 analysis The result screen displays Quality Control CAUTION A result will be accepted only if it is confirmed with the key 5 1 6 1 QC database The database of measured a
26. erent patient types a Child SETTINGS 4 LIMITS 4 FEMALE 2 Modify the displayed lower and upper limits using the numerical keypad Confirm data by pressing the OK key Pressing and function keys accesses additional pages DAA 16 0 1H 3 l 4 5H 5 50 iaia 14H 174 g l 45 0 52 8 5 84 96 fl T B 32 8 pg If 0 0 0 0 is specified for lower and 30a 358 g l upper limits that parameter will not be 150 400 189 1 verified AHH HHH X On the first screen of the limit settings the E software allows you to change the AH A fl amount of lyse added The instrument AD Ga x permits a range of 0 3 ml to 1 1 ml 28 68 42 8 fl The software contains default values that A A AA are optimal for individual species see 1 38 4 00 10 91 table below 6 15 6 78 109 1 AoA 7 560 10 91 25 0 48 9 3 B Tg K 50 0 Fo x J o Abacus junior User s Manual 40 Optimal quantities are strongly influenced by the chemical composition and behavior of the lysing reagent The quantity of the lyse reagent can also be modified before each analysis by t 0 1 ml without having to change the value in this sub menu Abacus junior User s Manual 41 6 DATABASE Patient results are stored in the memory in chronological order and can be retrieved at any time Memorizing capacity is 1 000 measurements including the complete parameter list histograms flags sample data and date time of measurements DATABASE
27. etrieved but can be reviewed in the VIEW CALIBRATIONS menu Calibration can be initiated by choosing Calibration in the MEASURE MENU Calibration Calibration can be initiated by choosing 1 Calibrate Calibration in the MEASURE SESS Item 1 opens the CALIBRATE menu level which is displayed in the following screen Item 2 displays only the pervious control calibration factors 3 Calibration settings 5 1 4 1 Factorial calibration lf the CALIBRATION MODE has been previously set to Factorial Calibration the factors can be set manually in the 0 80 1 20 range alan measure Control CALIBRATION 3 RBC CALIBRATE 1 MEY Enter previously calculated factors using the numerical keys confirm with the OK key FLT 5 1 4 2 Automatic calibration by measurement If the CALIBRATION MODE is set to one or three fold measurements calibration measurements are performed with a hematology control substance Calibration MEASURE RBC 457 10121 CALIBRATION 3 MCV CALIBRATE 1 automatic RDWc oet target values of the control material using the numerical keys Use the OK key to accept a value Specify O as target value for parameters that should be omitted from calibration After setting the values press to validate and start calibration measurements Abacus junior User s Manual 31 Target values for calibrated parameters can be set within the following ranges Parameter Lowlimit Highlimit
28. f the result screen the 11 5 Y overall test result is displayed which can be 12 1 Y Successful every test result is OK or Errors 3 5 V found if HIGH LOW or ERROR results 55 3 C appeared Overall result Success ul Abacus junior User s Manual 49 7 3 Printer Settings Make sure to select the appropriate printer mode for correct operation before printing The instrument supports the following printer modes and languages Selected mode Printer language Supported printers Built in Seiko Special language Built in thermal printer Canon BJC Canon BJC Canon BJ and BJC series Canon BJC in 9 pin mode dei Epson 9 pin ESC P EPSON 9 pin dot matrix series e g FX 980 Epson 24 pin ESC P2 EPSON 24 pin dot matrix Epson 24 pin in 9 pin mode series e g LQ 580 Epson Stylus Raster Epson ESC P Raster EPSON Stylus series e g C20 C40SX C60 C80 HP DeskJet HP DeskJet series e g DJ 920c 940c 960c HP LaserJet PCL4 HP LaserJet series and compatibles e g LJ1100 Seiko DPUA14 Special printer Seiko DPUA14 printer language Any printer compatible with the above listed modes printer languages can be connected to the instrument Abacus junior User s Manual 50 To set up the instrument for your printer go to the Utilities Printer Settings menu Select from the options using the up and down arrow keys within the text fields and fill in the numerical fields using the number keys The genera
29. hut down menu is Shutdown J changed 2 Logout Logout menu point appears 3 Prepare for shipment SHUT DOWN 6 A logged in user who has finished working with the instrument should select SHUT DOWN 6 When logout is used instead of shutdown the log in screen will be displayed for the next user Abacus junior User s Manual 58 7 5 Software Update The user following the steps below can easily update instrument software 1 Shut down the instrument and switch it off 2 Insert the upgrade floppy disk in the drive or a CD in the CD ROM drive optional 3 Switch on the instrument and follow the on screen instructions 4 When instructed remove the disk from the drive At the next start up the instrument will run the new software version 7 6 Fluidic Schematics Abacus junior X Fluidic Schematics Version 2 0 Micro X V7 DilChamber V9 LyseWbc 2 E Pressure Meter LDL Puffer Reservoir Lyse LYSE DILUENT XT V10 Cleaner Lyse Sensor CLEANER Macro Dil M3 DX wo V3 Bubble 2 l IN Sample 3 V5 DrainPuff D4 V1 DrainChamber y Ya reune rotor Hor Tie DX V2 DrainAperture Ver E m ri lt q CE Pump LDx Liquid Detector XF 2 way Valve ae 3 way Valve 3 WASTE Closed Off WP 13 0f M2 Stepper Motor Open On 2 3 On Abacus junior User s Manual 59 S 8 pRINTING This chapter covers information on
30. idth RDW CV absolute Platelet Distribution Width PDW P1 P2 ENG ASOMO xDW SD RDW a x P2 P1 fl xDW CV RDW aai x 0 56 x P2 P1 P2 P1 by the factor of 0 56 CV is corrected to the 60 cut Platelet PLT Number of thrombocytes platelets cells l cells ul PLT PLT ga x cells l cells ul fl the PLT histogram Thrombocrit PCT Calculated from the PLT and MPV values PCT percentage PLT x MPV x 100 percentage absolute PCT absolute PLT x MPV White blood cell differential Absolute values counted in the channels determined by LYM LY lymphocytes the three WBC discriminators MID MID monocytes and some eosinophils GRA GR neutrophil eosinophil and basophil granulocytes Percentages calculated from the absolute WBC value Abacus junior User s Manual 26 4 4 Absolute and Linearity Ranges of Measured Parameters Within the linearity range the instrument is guaranteed to provide the specified accuracy Beyond this linearity range the instrument is able to display results but may not guarantee accuracy characteristics If the value is over the maximum range of guaranteed linearity the instrument cannot measure it and the result will be marked with an E Error flag To measure a sample whose parameters exceed the maximum value indicated in the table below predilution is recommended See section 6 1 6 of this manual The linearity ranges of primary parameters in n
31. ifuges and shaker baths or alike Abacus junior must be plugged into a grounded AC outlet Do not plug the instrument into electrical outlets on the same circuit as devices that operate intermittently and use large electrical currents such as air conditioners refrigerators compressors etc It is not advisable to use extension cords especially multiple outlet extensions If you are located in a geographical area that experiences excessive power fluctuations or are using a generator connect the instrument to a surge protector filtered surge protector preferred Abacus junior should be installed on a flat level surface with adequate room for the reagents the optional keyboard and printer The backside of the instrument should be at least 6 inches from adjacent walls or other equipment to allow sufficient airflow and ventilation Abacus junior uses external reagent containers Care should be taken so that the table holding the instrument is not pushed against the wall blocking the tubes connecting the instrument and the reagent containers Abacus junior User s Manual 16 2 3 Unpacking and installation 1 Carefully remove the Abacus junior hematology analyzer from the shipping carton Carefully inspect the instrument for any visible signs of damage incurred during shipping If you find any damage immediately file a claim with the carrier or your distributor Check the accessories received against the packing list Contact your service pe
32. l characteristics of the printable area of printer paper are below The paper is defined by its size Paper width it can be a standard size A4 Letter or any custom sized HTop margin Physical margins paper specify the actual size Printers cannot print on the e Result whole surface of the paper The e 7 Printable area blank area is described by the nana tas physical Margins which may Vertical spacing vary by printer models The D paper area inside the physical Left margi Result margins is called the printable i area 5 Top margin and Left margin E settings are used for 5 determining the location of a printed results on the page A If more than one result is to be printed per sheet use the Vertical spacing to specify the distance between reports Frinter settings On the first page of the Printer Settings sub Printer menu printer type can be selected Here setko DPU414 you can choose between Seico built in Mode printer or any compatible external printer Hormal T In addition graphics mode and paper Physical margin E settings can be selected Paper Select the Printer matching your printer hardware Different Modes result in different printout sizes Choose the one matching your needs The available modes depend on the selected printer type Possible options are Mini Small Normal Enhanced and Large for the size Wide and Narrow f
33. les 3 lt 3 lt 1 4 0 20 0 3 lt 3 lt 1 4 00 15 00 3 lt 3 lt 1 25 0 50 0 2 lt 1 N A 50 90 2 lt 2 lt 1 9 16 4 lt 4 lt 3 200 900 Open tube system with automatic sample rotor Human general Male Female Baby Toddler and Child RBC WBC clogging 1 of analyses normal use High voltage pulse and chemical cleaning of the aperture in each analysis cycle using Cleaner reagent High voltage burst of aperture high pressure back flush chemical cleaning of aperture using Cleaner 6 levels including mean range SD and CV for all measured and calculated parameters 16 and 64 day Levey Jennings charts separate QC database Automatic based on 1 or 3 measurements or manual calibration of WBC HGB RBC PLT MCV RDW MPV separate factors for prediluted mode monitoring of calibration factors by calibration events 3 level multi user operation with selective privilege levels user identification with ID and password Easy to use menu driven user interface with 6 software buttons with icons 6 hardware function buttons above LCD cursor and numeric keys Chinese English French German Greek Indonesian Italian Persian Polish Portuguese Romanian Russian Spanish Vietnamese 1000 results including histograms Serial RS 232 computer link 3 5 floppy disk 3 5 floppy disk or slim CD ROM optional Centronics parallel or USB optional Easy Paper Operation built in
34. lysing sample An over lysed sample will have all its cells shifted down towards the smaller cells It is because the too much lysing reagent shrinks all cells to the size of their nuclei With lyse quantity decreased the cells will not shrink that much and can be differentiated easily Abacus junior User s Manual 34 5 1 5 3 Sample information The software allows the user to enter information for each sample that has been or will be measured If an external PC keyboard standard USA layout is used it must be connected to the instrument before turning the instrument on Two options exist for sample information entry e immediately before analysis e inthe Database menu To enter sample information prior to sample analysis press the function key The following screen appears Sample ID and patient data can be specified name sex date of birth Also the name of the doctor having ordered the laboratory analysis can be given The patient name will appear on the LCD below the PLT histogram second page and in the header of the printed result sheet Type in the name using up to 32 alphanumeric characters A Z 0 9 space comma dot and parentheses Use arrow keys to move between characters and the backspace for deletions Press Enter to accept data cancel with Esc or X J confirm with v J 5 1 5 4 Results At the end of an analysis the following screen is displayed including all measured and calculated p
35. n be 18 85 2883 retrieved Model name serial number software version and the date of the compilation of the software are available UTILITIES DIAGNOSTICS 2 STATISTICS 2 This menu includes important information Successful Start ps about analyses previously performed the successful ShutDowns total number and any clogging or errors Jlincurred Abacus junior User s Manual 48 7 2 2 Self test The Self test is a procedure to verify proper operation of essential components of the instrument The Self test should be performed e Atinstallation e After replacing any component e After extended time out of use The automatic Self test procedure can be initiated from the DIAGNOSTICS menu During the test you can see its progress The components that have passed the test get checked Self testresults The first panel of result screen includes the 11 85 2883 date of testing and device information Abacus Junior Human 16 AB 1 541 dev 10 06 2003 The second and third panels of the result screen include every test result At the end of the result line OK HIGH LOW or ERROR is Electr voltage 56 1 v displayed which means that the actual test current 898 ufi result is at the normal range OK higher offset Bi mv HIGH lower LOW than the pre determined Ampl test 19999 pls limits or the result is an error ERROR 1674 mw B3 mv ld pls Ssec 354 mbar 364 mBar o mbBar 1Hsec On the last panel o
36. nd stored QC results can be displayed at any time in table or graphic Levey Jennings formats The QC measurement results will have sequential ID numbers MEASURE 26 05 2003 B3 59F QUALITY CONTROL 4 26 65 2003 4 01 F VIEW TABLE OF QC BE B5 28183 04 03F MEASURE 3 BE B5 28183 04 06F Move the selection bar over entries with 96 05 2003 B4 B8F the T and keys gt 06 05 2003 04 10F Move among parameters with and gt CAUTION Any change in the QC material settings is followed by deletion of the QC database It is strongly recommended to print the database before making modifications The analyzer produces graphic figures of the primary parameters WBC RBC HGB HCT MCV and PLT Abacus junior User s Manual 38 MEASURE QUALITY CONTROL 4 E IEEE CIE VIEW QC DIAGRAM 4 FM a he i Means standard deviations StDev and Dev E are Bd coefficients of variation CVar are ial ee es 109 1 calculated based on the QC analyses The Ear Sihey aid DVarz 5B dotted lines delineate acceptable ranges on Levey Jennings charts ue SR Fe You can look at Quality Control information and results in table format as well To access this format select item 3 View table of QC measures of Quality Control 5 1 7 Prediluted mode The software has a special Prediluted mode useful in the following situations e Sample values exceed linearity see section 4 4 e Small sample volumes e Capillary bloo
37. nfirmed it with the key the instrument will prompt you for an empty disk 35 data record s will be saved on 1 disk s Insert an empty floppy disk M 5106 1036 dy Alas Possible error messages include Ho such type of data The instrument gives this warning in case you tried to save data of a day which had no data or no data are selected STOP Error 1300 12210 This warning appears if the disk you inserted Cannot write archive data Check disk had errors on it or was write protected It may be unformatted full or bad Do you want to retry Check the write protection of the disk or if necessary insert a new formatted disk Abacus junior User s Manual 44 l nerd Selecting item one 1 of the MAINTANANCE you can accesses this menu 7 1 Maintenance 1 Maintenance 2 Diagnostics 3 Frinter settings 4 Settings 5 Empty waste container b Service 7 1 1 Regular Maintenance Jobs From Maintenance submenu the user can initiate maintenance procedures such as cleaning priming draining Pano MAINTENANCE 1 1 UL Select the required submenu e rime diluent 3 Prime lyse 4 Prime cleaner 5 Drain chamber 7 1 2 Cleaning Select item 1 CLEANING in the above MAINTENANCE menu This action is recommended if clogging problems are experienced C error flag This is a relatively strong decontamination process cleaning out possible dirt or clog from the system using high voltage el
38. o account the specifications attached to them Important Sample tubes must be filled at least 5 mm height with blood otherwise correct sampling is not guaranteed To initiate analysis 1 Invert the closed sample tube 11 times to achieve a homogenous sample Do not shake the sample because micro bubbles can form inside causing erroneous sampling 2 Remove the cap It is very important because the tip will not pierce the cap 3 Position the sample tube in the sample rotor 4 Push START key The sample rotor will turn into inside of the instrument and the needle draws 25ul of sample from the tube The aspirating needle is retracted while its outer surface is automatically rinsed with diluent This insures a low carry over between samples After a few seconds the rotor turns out and the needle remains inside the instrument Now you can take out the sample tube from the adapter of the sample rotor Abacus junior User s Manual 28 5 1 2 Measure local menu From the Measure local menu you can access 6 sub menus On the upper part of the screen you can ia repeat last sample find a thin band showing the short cut key e Measure blank positions Each key is indicated by a black J Calibration band Where the band is interrupted means 4 tualitu control that the corresponding key is activated In Os Prediluted mode case above the print icon you see a black B Limits arrow means that you can print from that position Measure
39. o the left i e the different WBC populations are overlapped This can inhibit proper separation of WBC populations If this function is selected the L or L sign can be seen in the top left corner during analysis see the screen below Abacus junior User s Manual 33 alel MEASURE MEASURE By pressing the f arrow the user can switch on the lyse boost 0 1 ml and L appears The following analysis will be performed with additional lysing reagent The two important parameters influencing lysing are lysing time and lyse quantity You cannot change the lysing time as it is adjusted to the lysing reagent supplied by Diatron Above you could read that the lyse quantity can be adjusted either at patient limits or right before measurement In the following figures you can see examples of over or under lysed samples 41 34119 36 72 107 Under lysed sample some RBCs counted The same sample with lyse boost 0 1 ml as WBCs WBC 713 7 good 3 part differential WBC 16 9 LYM high Figure 7 Effect of under lysing sample You can see that a lower amount of lyse will result in a less differentiated curve If you increase the lyse quantity it will be more differentiated and well evaluated 100 l PA WBC b p 30 70 26 73100 Over lysed sample LYM and GRA overlapped The same sample with lyse decreased 0 1 ml WBC 20 6 poor 3 part differential WBC 21 0 good 3 part differential Figure 8 Effect of over
40. or the width and the optionally Fast mode Recommended mode is Normal Physical Margin is used to correct the printout for a printer having a smaller printable area A larger margin here refers to a larger physical margin Alter the normal setting only if the right side of the printed result is missing or it appears in the next line Abacus junior User s Manual 51 Select Paper type or enter correct Size parameters in inches or cm as specified at Settings Units Frintersettings On the second page of printer settings cm additional options can be selected 21 4 x 29 7H Top Vertical spacing Rollpaper If rollpaper is used instead of sheets set Rollpaper to Yes If all results are to be printed automatically after each analysis set Autoprint Yes Printout format can be specified as one of the following e Full with histograms e Text only e Short text only lf you set One result per page to Yes each analysis will be printed on a separate page Printer settings The third page of printer settings contains Autoprinte Ho options for inclusions in reports Printout format Harrow test only Table format Hormel One result per page Ha Printranges Graphical Print flags Yes Jj Print ranges can be set to None or to 4 51 5 64 which means that reference ranges specified in Settings Limits will be printed next to each measured parameter with numbers or the reference ranges can be prin
41. ormal measuring mode Linearity Ranges Maximum Unit 0 100 10 cells liter EN GM mc ons 2 ete mr omw 10 cese ws 953 Lower f ow f f a IC EN Table 2 Linearity ranges of parameters The linearity ranges for 1 3 predilution mode Linearity Ranges Maximum Unit 2 200 NE 10 cells liter 101 cells liter ICI Table 3 Linearity ranges of Pre dilution mode Abacus junior User s Manual 27 5 ROUTINE UTILIZATION and MEASURE 5 1 Measuring Process 5 1 1 Sample handling Since some time will usually elapse between collection of samples and counting it is necessary to preserve the sample with an anti coagulant to prevent large groups of cells forming into clots or lumps of cell matter that will clog the cell counter Choice of anti coagulant is very important as some anticoagulants will affect the shape and size of blood cells In general EDTA preferably sodium or potassium based is the only anti coagulant recommended for use with electronic blood counters Care must be taken when using homemade containers pre dosed with EDTA If the container is not filled completely with blood the ratio of EDTA to blood may reach a level which results in osmotic transfer from the RBCs shrinking them The ratio of EDTA to blood should not exceed 3 mg ml Generally we suggest using manufactured sample tubes containing the necessary amount of EDTA and when you take blood please take int
42. printer module 240x128 dots high contrast backlit graphics LCD 24 foil keys START button Standard PS 2 compatible keyboard Universal external 12VDC 8A power module 90 260V 50 60Hz 10W stand by 80W max Abacus junior User s Manual Operating temperature Dimension WxDxH Net weight 15 35 C 320 x 260 x 365 mm 12 kg 14 Abacus junior User s Manual 15 2 INSTALLATION 2 1 General information This chapter provides instructions for the installation of the Abacus junior hematology analyzer The procedures described below must be followed correctly to ensure proper operation and service Please carefully read and follow all instructions in this User s Manual before attempting to operate Abacus junior Abacus junior hematology analyzer is a precision instrument and must be handled accordingly Dropping or other improper handling of the instrument will disturb calibrated mechanic and electronic components and or cause other damage Always handle the instrument with care 2 2 Environmental factors Locate Abacus junior so that it will not be exposed to extreme temperature variations Temperature should be held relatively constant to obtain maximum reliability The ambient temperature range for the instrument is 15 C to 35 C Do not place the instrument close to any open windows ovens hot places radiators air conditioners or in direct sunlight Do not expose the instrument to vibrations from equipment such as centr
43. r s Manual 54 7 4 2 Units me CT SETTINGS 4 UNITS 2 cells Units can be set in a sequential order The PCT HET unit arrow on the right indicates that there are EDW FDW made more options for the entry Select among options using the and Y keys When 3 xw done press OK to open the next parameter When the last entry is set pressing confirms the data and returns the display to the Settings menu The possible units for above parameters are as follows cells liter cells l cells ul cells ul HGB unit grams liter g l grams deciliter g dl millimols liter mmol l PCT HCT unit percentage absolute abs RDW PDW mode standard deviation sd coefficient of variation cv Table 9 Selectable units of parameters related to HGB 7 4 3 Fluid Sensors Fluid sensors check for the presence of diluent cleaner and lysing reagents In the event of a malfunctioning sensor instrument operation can continue by disabling the defective component in this sub menu AAA SETTINGS 4 Dll Diluent amp Cleaner FLUID SENSORS 3 Ble Lyse 3 Calibrate sensors Each sensor state can be toggled on and off by using the OK key Here the diluent amp cleaner sensor is T disabled WwBCBlank measurement When any fluid sensor is switched off an Abd BH B3S3 warning appears in the upper left corner of d wIREC AAA MCHC the display during analysis The operator can utilize the soft
44. rson if anything is missing 2 CAUTION Prior to initial operation allow the instrument to warm up to room temperature for at least 6 hours Rapid temperature changes in an operational unit can lead to water condensation damaging electrical parts 3 Place the instrument on a firm work surface in the designated work area near an appropriate AC electrical outlet The connection MUST be grounded NOTE Before making connections Be certain that all power is in an OFF setting before connections printer external keyboard are made Carefully read all literature accompanying the instrument and its accessories Pay particular attention to the operating procedures for the external printer 4 Keyboard and printer Attach the keyboard cable to the round KEYBOARD port on the back of the instrument Attach both ends of the printer cable to the appropriate ports on the printer and Abacus junior Attach the AC adapter to the printer if required and plug it into an AC outlet 5 Host Computer The instrument has a built in serial port that allows connection to a host computer Results including histograms may be exported The serial I O settings can be found in the Service Menu System For installation instructions please contact your service person 6 Power supply Attach power cord outlet to the external power supply of Abacus junior and plug the other end into a properly grounded AC outlet Please do not switch on th
45. s the lower level and the right side shows the upper level The value in the normal range is indicated with a marker If normal ranges are set flags are enabled and patient values are over or below the limits specified the result out of the range is marked with an mark near the value and the rectangle is compressed because of lack of space and the high low value is indicated out of the rectangle on the right left side Abacus junior User s Manual 61 Revision history Revision Section Modification By At 1 1 All Menu system Losonczi 2003 06 12
46. screen is displayed EXIT SHUT DOWN 1 IM Shut down 2 Preparing for shipment The software will prompt you for confirmation Press 1 and the analyzer will perform a priming cycle filling the chamber to avoid dust and salt build up The screen will prompt the user again to power off Abacus junior User s Manual 20 2 3 3 Preparing for shipment The second item in the shut down menu should be used when the instrument is to be shipped or left unused for a longer time The instrument will ask you to utilize the cleaning tube kit and 100ml of distilled water Follow the instructions appearing on the display SHUT DOWN 6 Mi A a PREPARING FOR SHIPMENT 2 Remove reagent tubing at rear reagent inputs Diluent Lyse Cleaner Here the user is instructed to remove tubing connectors so they could be drained Leave the waste connector attached Next you should connect the cleaning tube kit to the reagent inputs submerging the free end in a bottle containing at least 100 ml of distilled water Message 5002 19300 Connect min 100 ml distilled water to reagent inputs using cleaning tube kit The analyzer will flush any remaining reagents from the system into the waste container Message 5003 19300 As a next step the analyzer asks you to remove the cleaning tube kit Remove cleaning tube kit Keep reagent inputs free When finished the analyzer prompts you to power off the system Remove the waste connec
47. ted out in graphical form If Print flags is set to Yes any measured value out of the reference range or error incurred during analysis be shown on the printout If Print warning flags is enabled any flags appearing on the result screen will be included on the printout Abacus junior User s Manual 52 7 3 1 Troubleshooting guide for printing problems Problem Possible reasons remedies Printer does not respond Printer is off Turn it on no printout Printer is not connected to the analyzer Connect it to the parallel port at rear Printer is not on line Switch it to on line mode Printer is out of paper Replenish paper otrange signs or letters The selected printer type does not match your appear on the printout printer Select the appropriate type in Printer settings menu e Printer is not set up properly for HP or Epson or compatible mode Modify the printer setup Consult the printer s manual Right side of the printed e Decrease Margin settings in the Printer Set Up report is missing or menu appears on the next line e Try changing Mode to a smaller printout small with excess space on the paper The end of the printout e Enter the correct Paper size appears on the next page e Try increasing Margin One more patient report e Enter the correct Paper size could fit on the same page e Try decreasing Margin Top margin Vertical spacing The printed result is not e Modify Left margin centered horizontall
48. tor after shutting down Abacus junior User s Manual 21 3 MENU SYSTEM 3 1 General information This chapter contains information about the structure and usage of the software implemented menu structure This integrated software controls instrument operations including calculation and evaluation of measured data displaying results and information screens storage and recalling of data 3 1 1 Navigating in the Menu System he instrument uses a menu system to initiate actions and allow settings modifications There are four possible ways to navigate between menus and menu items a he most important issue is that above the LCD screen there are 5 function keys you can navigate with them between menus amp e 64 Connecting a keyboard you can use F8 to F12 function buttons on the keyboard as well you will find in Menu Structure the corresponding key The big advantage is that these are short cut keys which means that by pressing any of them you can enter directly the menus wherever in other submenu you are With these keys you can navigate between menus in a so called horizontal direction In paragraph b c and d we present you the possibility of navigation in the deep direction b You can select the desired item with the 4 and y keys and press the OK key to enter or activate the highlighted item Within a submenu you can press the L2 function key to return to the previous menu level This method is suggested while
49. trol measurement For this reason no calibration or QC measurement can be performed without accepted blank values Quality control measurement and calibration can be performed only if all blank values are in the first region receiving no flags or errors 5 1 4 Calibration Calibration is the procedure used to standardize the instrument by applying the necessary correction factors Preferred hematology control is R amp D Systems CBC 3D Low Normal and High It is recommended to do calibration in the following cases 1 At analyzer installation before beginning the analyses 2 After replacing any component related to the process of dilution or measurement 3 When quality control measurements show any systematic error bias or they are outside predefined limits 4 Atregular time intervals determined by the lab itself 5 If you want to use the instrument in Prediluted mode Calibration can be performed in two ways 1 User can enter calibration factors without any calibration measurements using the numerical keypad 2 One two or three fold measurements of control or special calibrations material with known parameters In this case the instrument automatically calculates new factors using the following formula Assigned value x Stored factor New factor Measured value s or average of those Abacus junior User s Manual 30 CAUTION New calibration will invalidate the previous factors Old values cannot be r
50. ual 25 4 3 Parameters Abacus junior measures and calculates 18 different parameters listed below For each parameter you can find the name the abbreviation and the measurement unit in the first column You can find a short description for each parameter in the second column White Blood Cells WBC Number of leukocytes cells l cells ul WBC WBC a x cells l cells ul Red Blood Cells RBC Number of erythrocytes cells l cells ul RBC RBCga x cells l cells ul Hemoglobin concentration HGB Measured photometrically at 540 nm in each cycle blank measurement is performed on diluent g dl g l mmol l HGB HGBua X HGBmeasurea HGBolank fl from the RBC histogram Hematocrit HCT Calculated from the RBC and MCV values HCT percentage RBC x MCV x 100 percentage absolute HCT absolute RBC x MCV Mean Corpuscular Hemoglobin Average hemoglobin content of erythrocytes MCH calculated from RBC and HGB values pg fmol MCH HGB RBC Mean Corpuscular Hemoglobin Calculated from the HGB and HCT values Concentration MCHC MCHC HGB HCT absolute Unit of measurement is displayed according to the g dl g l mmol l one chosen for HGB result g dl g l or mmol l Red Cell Distribution Width RDW The distribution width of the erythrocyte or platelet SD fl population derived from the histogram at 20 of Platelet Distribution Width PDW peak SD fl minnie ieu ES n MN 10 RBC Red cell Distribution W
51. ument is maintenance free However would you experience leakage from the pump or vacuum error you may replace the tube used 1 Open the side door You can use a screw driver or coin to open counterclockwise 2 Open the pump lock by turning the plastic holders to release the pump housing plastics see figure 10 Snap out the pump tube yellow in color from the housing Pump housing plastics Pump tube Tube holder clip Tube holder clip Pump lock 3 Figure 10 Pump with open lock 3 Push the two ends of both clips to release the worn out tube 4 Connect the new tube and secure the connection with the two clips on both ends 9 Put back the tube into the housing plastics then guide in both ends into the lock 6 Turn back the lock to secure the tube in place Check the connections for proper placement Close and lock the side door in After replacement of tube the pump should look like the figure below 111317333153 Figure 11 Peristaltic pump after replacement of tube Abacus junior User s Manual 47 7 2 Diagnostics The DIAGNOSTICS submenu provides important information about the analyzer statistics and the built in Self test Diagnosis UTILITIES iB Device information DIAGNOSTICS 2 statictics This is the diagnostics menu 3 Self test Select the desired item UTILITIES Abacus Junior Human DIAGNOSTICS 2 16 DEVICE INFORMATION 1 ABJ 1 541 dev Here device specific information ca
52. ure Items 1 2 select between parameters 25 Calibrate with three measures Items 3 5 select between calibration modes hei UNT 77 item to perform CALIBRATION FOR PREDILUTED MODE When Calibrate prediluted mode is selected Calibration Prediluted mode will appear as the first line on the Calibration screens Previous Prediluted calibration factors cannot be monitored in the VIEW CALIBRATIONS submenu To perform factorial calibration enter reference parameters MCV and MPV or HCT and PCT and choose item 3 Factorial Calibration In this case User must have had the necessary amount of measurements with the control material based on which an average value can be calculated This average value is used for fine tuning the calibration parameters 5 1 5 Sample analysis 5 1 5 1 Sample preparation Use K3 EDTA anti coagulated fresh whole blood as sample Prior to sampling mix the sample gently by inverting it 11 times Do not shake as this could damage the blood cells 5 1 5 2 Modifying lyse quantity The default lyse quantity can be adjusted in the MEASURE LIMITS menu for each species An additional option to modify the lyse quantity by 0 2 ml is available during analysis Press T to increase the lyse quantity 0 1 ml if the separation between lysed RBCs and WBC populations is poorly differentiated resulting in increased WBC and LYM counts Press Y to decrease the lyse amount 0 1 ml if the WBC histogram seems to be shrunk t
53. user mode to designate a Supervisor on initial entry User settings USERS 5 SS I MULTI USER MODE 2 o 1 Single user mode e2 Multi user mode By selecting item 2 the instrument enters 3 Add new user multi user mode and a new item appears on the screen Add new user USERS 5 ADD NEW USER 3 User information User ID Name Frank Smith Level Basic Active Yes The software assigns an individual ID to each new user Password In the name field a user name of 32 characters can be specified You can use either the keypad or the external PC keyboard When the name is entered the level should be defined as Supervisor Advanced or Basic The default setting for Active is Yes Use this option if you want to disable a user The last data to enter is the password a maximum of 8 alphanumeric characters Abacus junior User s Manual 54 7 4 5 1 Multi user mode Login If the analyzer is in multi user mode a login User ID screen will be displayed during start up Password asking for a user ID and a password User name l After the User ID is entered and confirmed Login with the OK key the user name User ID 1 corresponding to the ID appears in the bottom Password line If the user name was correct enter the User name password and confirm with the function Frank Smith key If the password was correct the analyzer continues initialization and is ready to work Exit In multi user mode the S
54. ware to measure waste fluid generated If the waste container capacity is set at installation Maintenance menu volume measurement commences and a warning message appears to prompt the user to empty the waste tank when full Abacus junior User s Manual 55 e Always use a waste tank that equals or exceeds the volume of the tank initially installed e Empty the waste tank when the warning waste is full is displayed e Reset the software waste counter after replacing or emptying a tank 7 4 4 Laboratory This menu allows entry of laboratory information These characters will be printed in the header of reports generated by the instrument Setlaboratoryname SETTINGS 4 The name of the laboratory is LABORATORY 4 gbacus Junior vet A maximum of 40 characters can be entered in each line The operator can enter this data by either using an external This name will be printed in the header acies Pi biais EF gt Connected to the instrument or by scrolling lto each letter using the and y keys Move between character places using the and gt keys 7 4 5 User modes Selecting item five 5 of the Settings menu you access this menu where you can enable Multiuser Mode of Abacus junior CAUTION Multi user mode has both advantages and disadvantages As it is a security option maximum care should be taken when using it It is based on operator ID s and passwords Would any operator forget th
55. wise accuracy cannot be guaranteed Diluent Isotonic saline solution used to dilute whole blood specimens and to rinse the fluidic system between measuring procedures Lysing reagent Used to create hemolysate for 3 part WBC differential and for total WBC and HGB Cleaner Used to perform cleaning process of the fluidics 1 2 4 Technical Operation As Abacus junior is a fully automated instrument operating it requires minimal training or technical support Operator interaction is reduced to the following e Perform a Blank Measurement before starting the daily routine e Enter sample and or patient data e Apply the sample for analysis e Print results either one by one or by selecting records from the database e Perform a simple weekly maintenance as described later in this description 1 2 5 Calibration and Quality Control Abacus junior arrives at your laboratory factory calibrated and ready to use However calibration needs updating whenever you find that the results have slightly changed or a different or new control material is used On each control material you receive to be used with the instrument you will find a control sheet listing the parameters the instrument should match Perform these calibrations as explained in a later chapter Quality Control is used for checking for proper calibration and performance of the analyzer Running these samples should happen on a regular basis as also explained in a later chapter
56. y The printed result is not e Modify Top margin centered vertically The distance between two e Modify Vertical spacing results is too small or too big After printing the printer e Common in bubble jet or laser printers does not eject the paper Do not repeat printing When the page is full or you leave the menu the printer will eject the paper automatically Abacus junior User s Manual 53 7 4 Settings Selecting item four 4 of the UTILITIES accesses this menu Settings The SETTINGS sub menu allows the user Date and time to set the lyse amounts to set the dates and 2 Units the user modes 3 Fluid sensors 4 Laboratory 5 User modes 7 4 1 Date and Time The date and time of each analysis is stored with the results This menu allows setting the built in clock and the format of the date displayed Date and time SETTINGS 4 BER set date and time DATE AND TIME 1 2 Day Month ear By selecting item 1 you will enter date and Q3 Month Day Year time setting mode next screen O4 Year Manth Day ltems 2 4 are radio buttons only one can O5 24 hour format be selected 26 12 hour format Set date and time SETTINGS 4 DATE AND TIME 1 SET DATE AND TIME 1 Enter the date and time using the numeric keys Date format can be set in the previous menu Confirm them by pressing the button The current date and time are 11 46 4445 B2a5 TP Abacus junior Use
57. yed The software version number appears few seconds later when the software starts When the start up procedure is completed 15 85 2883 02 44F the DATABASE MENU will be displayed 16 05 2003 00 37A 26 05 2083 12 38F 05 06 2003 10 56 16 06 2003 03 29P 10 06 2003 03 53F CAUTION Wait 5 minutes before initiating any measuring process to allow the instrument to reach the optimal working temperature In some cases a priming cycle is necessary prior to sample introduction The instrument will perform the cycle automatically if the fluid sensors are on and additional liquid in the tubing system is required A priming cycle should be run e at installation e incase of extended time out of use e after replacement of any component related to the Fluidic system Abacus junior User s Manual 19 2 3 2 Turning the Instrument OFF The instrument should never be switched off by simply flipping the power button on the rear panel Doing so may result in erroneous operation during later use It can be SO because the instrument uses different kinds of solutions one of which is the so called diluent This liquid is an isotonic saline solution containing salt Would it not be washed out of special units of the instrument or would chambers not be filled with this solution may lead to dust condensation or salt build up Therefore always follow the instructions below when switching the instrument off Select EXIT key ep The following
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