User Manual - Spectra Services
Contents
1. EMI FILTER INLET 2800502 4200505 WHITE 14 BLACK 14G WHITE 146 VASTA eh GREEN LIGHTED POWER SWITCH 7850570 0 BLACK 14G CHAMBER FAN WHITE 149 4880551 TB1 1 BLACK 14 2 i TB143 AIR PUMP 6700559 BLACK 14G GREY 14G AIR WHITE 146 15245 PUMP GREY 14G WHITE HT 14G TB2 8 CONTROL 60W 120 HEAT LIGHT 4 LI LI 4650554 TB1 4 1750500 230v120vcom SENSOR 46 2350502 100500 S LI WHITE 14G 446 RED HT 146 SAI ORANGE 14G RED 146 BLACK 14G WHITE 146 AIR 4650554 6 WHITE 140 ga zl CO LIGHT 4650554 78 49 amp E E CONNECTOR OW ia 9 TIB 13 N2 LIGHT 4650554 2 3 BLUE 35995 ali PURGE LIGHT 4650550 1401 78247 COMMUNICATION 98 TB1 2 1750973 g NEUT TTB2H2 5ROWN e 8 J2 E e 9 SOLENOID 6 VALVES TEMPERATURE LOAD 1 m 8600529 SENSOR MAINTEMP m 3 O2 DISPLAY 3 sensor WHITE 140 1750974 4 LOOP CONTROL e BROWN 14 e BOARD SL 1750972 lt LOAD 3 Ir _ JB CONTACTS LOAD 4 25 8 5 J7 YELLOW 14G AES m E CO2 DISPLAY E BLACK 14 1750975 z m 5 B gt WHITE 14G BLACK 14G nd 02 SST SENSOR 66005322. PURGE SWITCH Tp TTB1 9 TTB1 12 TTB1 110 1 10 __ 225__ BROWN 22G RED 226 2 SENSOR pa TTB1 15 TTB1 16 ware WHITE 14G 19 8320510 BLACK 186 e TTB1 5 NE TTB1 7 2 El TTB1 8 THERMOELECTRIC TTB1 6 BLACK 220 ma CHILLER EE RED 186 80K OHMS 1070502 WHITE 1468757416 BLACK 18G BLACK 18G RED 18G LIGHT SWITCH 6 4 64 7850579 12VDC WHITE 18G RED iSc of oce DER POWER Dodo DA TTB1 3 Ogre SUPPLY 6750514 LA EE DI DI P TW WWW LED LIGHT BAR 4650547 OUTLET 6100531 Bactrox Operations Manual 22 BACK PANEL BACTROX 9851359 1 3 1 4 1 6 1 7 1 8 1 11 1 12 TTB1 13 TTB1 14 TTB1 15 TTB1 16 7 1181 5 7 TT81 10 SS S N TEMPERATURE BROWN 24G SENSOR TTB 1 N N BLACK 24G 8000547 BLACK dN3L OL WHITE 241 NOLLOANNOO SSNA AWidsid 3 HOSNIS 9 HOSNIS zo HOSNIS NOLLYOINNNINOO HOLO3NNOO 68d OL RED 24G BLACK 18G BLACK 18G RED 18G BLACK 24G RED 18G RED 18G SLOVINOO wavy 4 LOOP CONTROL BOARD SL 1750972 QquoHiuva3 24VDC POWER 12VDC
3. PURGE SWITCH 7850531 LIGHT SWITCH 7850579 CONN J12 E TO RS232 f SOCKET J10 25 Bactrox Operations Manual CONTROL PANEL BACTROX 2 9851365 TEMPERATURE SENSOR 8100500 1 3 TTB1 4 lt TTB1 15 lt srewnzse TTB1 16 wsitezae TTB1fH34 8BtAeKco48 TTB1414 amp nRED24G RE OTP CONTROL 1750500 230V 120V COM hh BLACK WHITE RED RED fata LED LIGHT BAR 4650547 ORANGE LED CABLES 1800534 RED 188 BLACK 18G AIR PUMP LED LIGHT BAR 4650547 a RE LED LIGHT BAR 4650547 BROWN POWER CORD POWER CORD lt WHITE BLACK POWER SWITCH 7850570 HEAT LIGHT ae LIGHT 4650554 4650553 TEMP DISPLAY 1750973 Di N2 Teen 4650554 4650554 02 DISPLAY 1750974 AIR
4. BACTROX HYPOXIC CHAMBERS OPERATION MANUAL MODEL BACTROX Sheldon Manufacturing Inc P O Box 627 Cornelius Oregon 97113 EMAIL tech Shellab com INTERNET http www Shellab com 1 800 322 4897 503 640 3000 FAX 503 640 1366 Rev 8 25 2014 4861671 Contents OPERATION MANUAL urinaria 1 1 ORIENTATION iaia 3 2 RECEIVING AND INSPECTION sonia iii 6 3 INSTALLA TION rc I 6 4 CONTROL PANEL OVERVIEW lilla 8 5 HOSE AND ELECTRICAL 8 6 THERMOELECTRIC CONDENSATE CONTROLER eene 9 7 INCUBATOR TEMPERATURE CONTROLLER SET OVER TEMPERATURE 9 8 CO2 CONTROLGCSALIBRATFION orta nia 10 9 OXYGEN CONTROL CALIBRATION iaia ck iranica 11 10 POWER ONTHEBACTRO iraniani 13 TT 14 12 PASS BOX OPERATION nance dio 14 13 ENTERING THE icit rera Reel ak d hace 15 14 EXITING THE CHAMBER iicet n raihi iuo Bale aaa 15 15 MAINTENANCE c 15 io ACCESSORIES eT 16 17 TROUBLESHOOTING 4 cri 17 18 OPERATION TES eredi ond rl arti 19 UNIT SPECIFICATIONS italioti e iiid 20 20 DIAGRAMS 21 21 PLUMBING DIAGRAM airline 31 22 BAGTROX SPARE PARTS rh dc nto rire ion rn b
5. CHILLER amp OUTLET BACTROX 9851362 OL OL ALL END STRIP 375 AND SOLIDER TIN MOVIE MOTISA N33HDO OL ALIHM 29 Bactrox Operations Manual CHILLER amp OUTLET BACTROX 2 9851367 N 5 S m m m ALL END STRIP 375 AND SOLIDER TIN O O 9 g 5 a x 4 gt a 2 Bactrox Operations Manual 30 PLUMBING DIAGRAM 20 co2 N2 SAMPLE PORT 02 CALIBRATION PORT CONDENSTAE BOX GAS FILTERS FITTING LIST cali 3100518 X 1 PCS 3100519 X 9 PCS 3100516 X 5 PCS 3100517 x 10 PCS 3100698 X 1 PCS PASS BOX VENT SOLENOID VALVES AIR PUMP CONDENSTATE DRAIN TUBE MAIN CHAMBER MAIN CHAMBER VENT SET 10 PSI REGULATORS 7150510 SET 10 PSI PASS THROUGH BOX 31 Bactrox Operations Manual 21 BACTROX SPARE PARTS LIST DESCRIPTION PART NUMBER PART NUMBER QUANTITY 115 VOLT 220 VOLT CHECK VALVE FILTER 8600573 8600573 1 CONTROL OTL SAFETY 1750500 1750500 1 CONTROL PLC 1750621 1750621 1 CONTROL TEMPERATURE 1 CONTROL PROBE OTP SAFETY 8100500 8100500 1 ELEMENT 2350502 2350502 2 GASKET 9 X 9 SOFT LT BLUE 3450506 3450506 1 LIGHT LED 4650547 1 LIGHT OTP SAFETY 4650553 4650553 1 OTP SAFETY ALARM 0250501 0250502 1 OUTLET VACUUM PU
6. POWER SUPPLY SUPPLY 6750516 6750514 Q 00 MOTISA ania RED 146 8000542 BROWN 24G WHITE 24G 80K OHMS BLACK 14G WHITE 140 BLACK 18G PURGE TIMER WHITE 14G 8250513 4 BROWN 14G WHITE 14G TTB2 4 0 T1823 2 2 YELLOW 14G H o s 2 TTB2 1 B B 7 8000547 SOLENOIDS 8600528 N2 PURGE N2 Bactrox Operations Manual 23 BACK PANEL BACTROX 2 9851364 TTB1 12 TTB1 13 TTB1 14 TTB1 15 TTB1 16 TTB1 8 TTB1 6 1 7 1 4 Z 1181 5 DO Qu 1181 11 TEMPERATURE TTBI BROWN 246 SENSOR NO BLACK 24G 2 8000547 OG 24 pig LEE 9 COSO OCA 2 gt Jig J17 J8 _ ssng 8 8 59 GR oF og 55 Seed 2 5 al 515 2 BED 24G E 5 g eja BLACK 246 g o o o 4 LOOP CONTROL BOARD SL 1750972 00000000 5 5 2 3 35 55 gt sf 20 So 12VDC 24VDC POWER POWER SUPPLY SUPPLY 6750516 6750514 Q 0 80K OHMS O BLACK 18G soglie ale PURGE TIMER 3 WHITE 14G 8250510 BROWN 146 Srl MOTISA ana RED 146 8000542 BROWN 24G WHITE 24G BLACK 14G WHITE 140 WHITE 14G TTB2 4 Qu 17823 TTB2 2 Qu re TTB2 8000547 BLUE 146 BROW
7. SET OVER TEMPERATURE safety thermostat has been activated and taken control of the heating element During normal operating conditions this indicator should never be on OXYGEN CONTROL This controller is marked SET OXYGEN and indicates the percentage of O2 content within the chamber to 0 1 The UP DOWN buttons are used to input the set point calibrating the display and muting or unmuting the audible alarm The HIGH and LOW alarm indicators will light whenever there is an alarm condition associated with the 02 within the chamber The MUTE indicator will light whenever the audible alarm has been deactivated N2 INJECTING This pilot lamp is on whenever the oxygen control has been activated and is actively injecting N2 gas via the solenoid valve AIR INJECTING This pilot lamp is on whenever the oxygen control has been activated and is actively injecting air via the solenoid valve associated with the air supply pump CONTROL This controller is marked SET and indicates the percentage 2 content within the chamber to 0 1 The UP DOWN buttons are used to input the set point calibrating the display and muting or unmuting the audible alarm The HIGH and LOW alarm indicators will light whenever there is an alarm condition associated with the CO2 within the chamber The MUTE indicator will light whenever the audible alarm has been deactivated CO2 INJECTING This pilot lamp is on whenever the Carbon Dioxide co
8. supply Set the CO control to the desired set point using the up and down arrows The CO level comes from the factory preset to OFF Adjusting CO Display After the Bactrox has had several hours to stabilize at the desired CO set point and measure the actual CO2 with a Fyrite gas analyzer If there is any difference between the Fyrite gas analyzer and the display use the procedure described in section 7 page 14 for calibrating See Section 9 Page 19 next page for Fyrite use and instruction NOTE When using the Fyrite gas analyzer insure that gas is not being injected while the reading is being taken Always change the CO set point to 0 0 prior to taking the sample and change the set point back to the desired value after the use of the Fyrite gas analyzer is finished 8 4 Using a FYRITE to Measure Levels A Bacharach FYRITE Gas Analyzer is recommended to measure CO concentrations in the Bactrox chamber This test instrument is not supplied with the Bactrox Operations Manual 10 Bactrox but is readily available from your dealer Follow the instructions provided with each Fyrite instrument carefully to ensure correct and accurate readings Fyrite Quick Overview Press button on top of Fyrite canister to release concentration RELEASE BUTTON and tip canister to the side to ensure all fluid is released from the top of the canister Return canister to the upright position Loosen screw on slide sca
9. 32 Bactrox Operations Manual 2 IMPORTANT READ THIS INSTRUCTION MANUAL IMMEDIATELY Your satisfaction and safety require a complete understanding of this unit including its proper function and operational characteristics Be sure operators are given adequate training before attempting to put the unit in service NOTE This equipment must be used only for its intended application any alterations or modifications will void your warranty 1 ORIENTATION Document Chart holder Control Panel BACTROX GAS Connections Incubator Oxygen Sensor amp Box Pa s Box Arm Port Door Arm Ports Storage With Sleeve Bactrox Operations Manual 3 Figure 1 Sleeve Cuff Assembly The Sleeve Cuff Assembly consists of a neoprene sleeve a plastic cuff ring O Ring and a soft rubber cuff Unit comes with Latex Gloves and Cuffs Nitrile Gloves and Cuffs may be ordered from the Parts List The assembly allows gloveless chamber operation Replaceable Arm Port door O ring seals Figure 2 Arm port Doors CAUTION The Bactrox chambers have an efficient method of sealing the chamber by utilizing the Arm port doors as shown in Figure 3 The tightening knob on the Arm port doors should be turned just until the knob begins to grab DO NOT OVER TIGHTEN Damage can occur if too much force is placed on the Arm port door assembly Bactrox Operations Manual 4 Oxygen Sensor and Box In
10. The US market units are designed for 120 VAC 60 Hz 12 0 Amps The EU market units are designed for 230 VAC 50 60Hz 6 0 Amps If supplied with a detachable cord set plug the female end into the inlet on the unit and the male plug into the supply Assure that units requiring fuses have them installed The fuse is a T type 12 5 Amp for 110 volt units 10 Amp for 220 volt units This fuse may be at the inlet or a part of the cord set male plug Leveling The unit must sit level and solidly Leveling feet are supplied and must be installed in the four holes in the bottom corners of the unit With the feet installed and the unit standing upright each foot can be raised by turning it in a counterclockwise direction Adjust the foot at each corner until the unit stands level and solid without rocking If the unit must be moved turn the leveling feet in clockwise all the way to prevent damage while moving Lifting and Handling CAUTION Use appropriate lifting devices that are sufficiently rated for these loads Failure to do so could result in minor or moderate injury Units should only be lifted from their bottom surfaces Handles and knobs are not adequate for lifting or stabilization The unit should be completely restrained from tipping during lifting or transport All moving parts such as trays or covers should be removed during transfer to prevent shifting and damage Cleaning Appropriate lab practice includes cleaning and disinfecting the un
11. calculated for your present environmental conditions and a tank of certified tank of oxygen and nitrogen mixture An example would be a dry 5 oxygen and 95 nitrogen mixture Offset calibration can be done through front panel display buttons See the OFFSET Calibration section below To complete a two point linear calibration the Bactrox needs to be hooked up to a computer that has RS 232 capability with windows terminal or internet access to download TERATERM off the web Calibration should be done before chamber use To calibrate first install the calibration cover onto sensor box in figure 1 using the two screws provided Figure 8 Bactrox Operations Manual 11 9 5 TWO POINT CALIBRATION PROCEDURE 9 5 1 9 5 2 9 5 3 9 5 4 9 5 5 9 5 6 9 5 7 9 5 8 9 5 9 9 5 10 9 5 11 9 5 12 9 5 13 9 5 14 9 5 15 9 5 16 The two point calibration procedure requires a terminal emulator program Shel Lab recommends the free open source emulator Tera Term Tera Term can be downloaded for free from the developers project page http ttssh2 sourceforge jp index html en Follow the instructions to download and install the emulator Once the software has been installed hook up the RS232 DB9 cable to the back of the oxygen controller If the computer has a RS232 port plug the other end of the 089 cable to the RS232 port If the computer has only USB use the USB to RS232 adaptor provided with the calibration
12. display will revert to showing the process or actual parameter within the incubator chamber 11 MAKING THE CHAMBER HYPOXIC 11 1 11 2 11 3 11 4 NOTE Prior to Purging the Chamber Turn Power switch to the O Off position Connect Nitrogen and Carbon Dioxide Gas supplies as directed in the Bactrox Appendix Open the incubator doors before leaving the chamber This allows the incubator to become hypoxic at the same rate as the workspace Put arm port doors in place or the Arm Port Sleeve Plugs The Bactrox chambers have an efficient method for sealing the chamber by utilizing the Arm port doors as shown in Figure 3 The tightening knob on the Arm port doors should be snug DO NOT OVER TIGHTEN Damage can occur if too much force is placed on the Arm port door assembly When the knob just begins to grab the tightening bolt has reached its maximum point Over tightening will cause the bolt to slip inside the handle and cause leakage Purging the Chamber 11 5 Power Switch to the I On position on the Control Module 11 6 Note the reading on the gas regulators 11 7 Set the Oxygen Setpoint to the desired value using the up and down arrows 11 8 X Set the Carbon Dioxide Setpoint to the desired value using the up and down arrows 11 9 Oxygen controller will automatically add Nitrogen gas or ambient air to achieve the desired setting Normally it takes 1 1 2 to 2 hours to achieve a 1 concentration o
13. plumbing connections panel Install one end of the hose from the CO gas regulator of the gas tank to the port marked CO2 PORT on then plumbing connections panel NOTE CONNECTING HOSES Hose connections are intended to be simple Insert the hose into the barb until the hose stops 5 7 5 8 Plug power cord from Bactrox into a suitable outlet Refer to the Installation Section of this manual for additional information Turn the power switch ON to verify power to the system When the power switch to the Hypoxic Chamber is activated you should see a light in the back lit power switch the 02 and CO2 digital display panels illuminate along with a readout of the incubator temperature If any of these appear not to work consult the Troubleshooting Guide 6 THERMOELECTRIC CONDENSATE CONTROLER 6 1 6 2 6 3 6 4 The thermoelectric condensate controller collects excess humidity and eliminates chamber condensation Moisture is funneled into a tube for collection inside your chamber The thermoelectric condensate controller is located on the left side of the chamber interior behind circulation fan A plastic tube drains excess moisture into the working chamber on the back left side Place a container e g glass flask or beaker under the tube for collection Empty the container DAILY Desiccants are not recommended in the chamber Desiccants are drying agents that will pull moisture from your samples by removing humidity
14. Check incubator temperature Record gas tank readings Change Rejuvenate Anatox Anatox absorbs volatile fatty acids and hydrogen sulfide in the chamber Recommended use is 250 grams placed in a beaker in the chamber Anatox 1000 grams packaged in 250 gram packages Instructions Empty two 2 250 gram packages into two 2 separate 500 ml beakers Day one place one beaker the chamber new Anatox can be placed directly into the chamber Bactrox Operations Manual 15 Day two remove beaker the chamber replace with the second beaker of also new Day three reactivate the first beaker of Anatox by heating 160 C for 2 hours Then replace the beaker in the chamber with this reactivated beaker Day four six months continue switching the reactivated beakers daily until six months is complete then discard the old Anatox and repeat the procedure again 16 ACCESSORIES Tank Switch Switches and monitors dual gas tank supply Provides automatic switch over to second gas tank alarm sounds when both tanks are empty Tanks can be switched manually at any time Model 2002 B Nitrogen Gas Regulator Optional accessory used in a two gas chamber Part 7150500 Cleaner Benzalkonium Chloride Chamber Stand Provides work bench to support Bactrox chambers Chamber Stand height 30 Stands feature locking casters and storage space 2 Gas Regulator Op
15. LIGHT CO2 LIGHT 4650554 PURGE LIGHT 4650550 CO2 DISPLAY 1750975 PURGE SWITCH 7850531 TOBUSS CONN J12 BROWN 24 LIGHT SWITCI 7850579 RED SOCKET J10 26 Bactrox Operations Manual INCUBATOR amp FAN BACTROX 9851361 EC YALOL IOHLNOO 410 OL 1091400 10 OL 8 91 MOVIE FAN PIGTAIL CORD 1800536 CHAMBER FAN 4880550 YOSNAS ALIHM po Ne Cu 7 S Es 8 lt Ob S e 5 2 8 8 O 8 5 27 Bactrox Operations Manual INCUBATOR amp FAN BACTROX 2 9851366 eitval OL EALEALOL IOHLNOO 410 OL IOHLNOO 410 OL 8 91 OL De MOVIE Q3H MOTISA N33HDO ALIHM FAN PIGTAIL CORD 1800536 CHAMBER FAN 4880551 YOSNAS 7 e S a 5 5 lt 9 co 8 3 28 Bactrox Operations Manual
16. MP 6100525 6100531 1 POWER CORD 1800516 1800541 1 POWER CORD BACLOOP LIGHTBOX 1800516 1800541 1 SOLENOID CHAMBER GAS 8600528 8600529 1 SOLENOID GAS1 8600528 8600529 1 SOLENOID GAS 2 8600528 8600529 1 SWITCH AUTO START RESET 7850531 7850531 1 SWITCH BACLOOP LIGHTBOX 7850579 7850579 1 SWITCH CHAMBER PRESSURE 7850524 7850524 1 SWITCH LIGHT 7850529 7850529 1 SWITCH MAIN POWER 7850570 7850570 1 SWITCH MANUAL VAC GAS 7850530 7850530 1 PE CONDENSATE 1070502 1070502 1 Bactrox Operations Manual 32
17. N 14G BLUE 14G YELLOW 14G SOLENOIDS 8600529 Bactrox Operations Manual 24 CONTROL PANEL BACTROX 9851360 TEMPERATURE SENSOR 8100500 TTB1 3 TTB1 4 1 16 lt wrttezag TTB1 13 lt stack 240 TTB14H4 4 ntgD2468 ELEMENT 4 REE HE OTP CONTROL 1750500 230V 120VCOM LED LIGHT BAR 4650547 RED ORANGE AIR PUMP 6700558 LED CABLES 1800534 18G BLACK 18G EEE LED LIGHT BAR 4650547 LED LIGHT BAR 4650547 TTB2 2 BROWN ig TTB2 3 TTB2 4 YELLOW POWER CORD HITE POWER CORD lt 8tack _ WHITE BLACK POWER SWITCH 7850570 TO BUSS T HEAT LIGHT LIGHT 4650554 4650553 TEMP DISPLAY 1750973 N2 LIGH 4650554 moet AIR LIGHT 4650554 02 DISPLAY 1750974 Qe CO2 LIGHT N 4650554 PURGE LIGHT 4650550 CO2 DISPLAY 1750975
18. O ssna SENSOR MAIN TEMP 8 SENSOR 02 SENSOR J17 co2 SENSOR J16 COMMUNICATION EARTH NEUT J2 BLACK 24G RED 24G HOT Ji LOAD 1 J4 LOAD 3 J6 4 LOOP CONTROL BOARD SL 1750972 CONTACTS BLUE 14G BROWN 14G 6700558 AIR e WHITE 140 TB2 9 PUMP WHITE HT 14G TB2 8 HEAT LIGHT WHITE 140 5 4 OTP LIGHT 4650553 WHITE 140 AIR LIGHT 4650554 SOLENOID VALVES 8600528 ALARM LOAD 4 7 1 9 7850531 TTB1 12 BROWN 22G 1 10 RED 226 TTB145 e a TTB1 7 TTB1 8 TTB1 6 LIGHT SWITCH 7850579 RED 186 RED 186 TTB1 4 BED 18G a 1 3 0 meme operer PURGE SWITCH PURGE TIMER 8250513 BROWN 22G WHITE 226 WHITE 22G BROWN 220 BLACK 18G OUTLET 6100529 3LIHM WHITE 14G THERMOELECTRIC CHILLER 1070502 WHITE 146 12VDC POWER SUPPLY 6750514 WHITE 146 WHITE 14G WHITE 18G WHITE 18G Bactrox Operations Manual 1 1 TB1 3 TB1 6 2412 TB2 1 0 TB1 5 TB2 7 1B1 2 HM all TB7 1 8 7 13 ITB8 2 TB7 16 1TB8 21 TB714 TB8 24 WIRING DIAGRAM BACTROX 2 9851363
19. PASS BOX for 45 seconds The PASS BOX PURGING light will alternately flash on and off indicating the cycle is in progress When the cycle is complete the system will stop automatically and the PASS BOX PURGING light will turn off This light will NOT remain on after a cycle is complete 12 3 3 If the cycle does not follow the sequences in the previous section on consult the Troubleshooting Guide for adjustments NOTE Do not hold START PASS BOX PURGE switch down Simply Press the button and let go to activate 12 3 4 If you want to abort the cycle press and hold the START PASS BOX PURGE switch for 5 seconds Then the PASS BOX PURGING pilot light will stop flashing 13 ENTERING THE CHAMBER 13 1 13 2 13 3 The rubber cuffs of the sleeve should be secured around your arm as opposed to clothing Remove sharp objects such as rings watches bracelets etc as they may damage the cuffs Insert your hands and forearms into the sleeves The cuffs must be firmly secured around your bare forearms Loosen the arm port door knobs a few turns by turning the black wing nut counterclockwise and rotate the locking bars to a horizontal position The arm port doors can now be pushed forward into the chamber interior and can be secured on the arm port door holders NOTE Arm movements that are too forceful may cause the sleeves to dislodge from the arm ports 14 EXITING THE CHAMBER 14 1 14 2 14 3 14 4 Doors are used to
20. PASS BOX the cycle can be aborted in order to eliminate the unnecessary use of gas Chamber Checklist It is recommended that customers review a checklist before they enter the Bactrox Chamber The checklist serves as a reminder of supplies and samples they need to bring into the unit Common items listed on a checklist are re activated anatox samples and loops The checklist can be placed on the front Plexiglas panel Size of Sleeve Cuffs It is critical that the sleeve cuffs seal around the user s arm during chamber operation to ensure the desired hypoxic environment The location that the cuffs seal on the users arm is determined by individual preference The size of the cuffs that come standard with the Bactrox Chamber is medium size 8 glove Other sizes are available large size 9 and small size 6 Replacement of Cuffs When and how often the cuffs on the sleeve need to be replaced depends on customer care and technique Typically the cuffs are replaced about every three months Customers should watch for small holes that can appear where the cuffs are placed on the ring cuff If holes are present the cuffs should be replaced to avoid gas leak Watches and jewelry can damage the cuffs and are not recommended to be worn during chamber use Bactrox Operations Manual 19 UNIT SPECIFICATIONS Model Number Interior Exterior Chamber
21. POWER The I O ON OFF switch controls all of the power for the chamber and must be in the position before any systems are operational Both SET TEMPERATURE SETO2 90 SET CO2 displays will illuminate when the power switch is in the ON position SET TEMPERATURE This controller is marked SET TEMPERATURE and indicates the actual temperature within the chamber to 0 1 C The UP DOWN buttons are used for inputting the set point calibrating the display and muting or unmuting the audible alarm The HIGH and LOW alarm indicators will light whenever there is an alarm condition associated with the temperature within the chamber The MUTE indicator will light whenever the audible alarm has been deactivated HEATING ACTIVATED LIGHT Heating activated light will be lit whenever the main incubator heating element is on SET OVER TEMPERATURE This is a hydraulic thermostat that is wired between the Main temperature controller and the heating element and functions as an override control During normal operation if at any time the SET Temperature control fails in the ON position and the temperature in the incubator rises above its set point the SET OVER TEMPERATURE is activated and maintains temperature at its own set point Note that the HEATING ACTIVATED LIGHT indicator will not continue to function while overridden by Over Temperature Limit OVER TEMPERATURE ACTIVATED LIGHT This pilot lamp is on whenever the
22. Shipping Unit Dimensions Dimensions Capacity Weight Weight WxDxH WxDxH Inches Inches BACTROX 2 48 5 x 31 0 x 32 6 300 plates Model Number Temperature Range Temperature 02 Range CO2 Range Conirol Uniformity BACTROX Ambient 8 to 60C S BACTROX 2 0 1C 0 35C 37C 0 20 0 20 Electrical Model Volts Amps VA Cycles BACTROX 120 3 5 1100 50 60 hz BACTROX 2 240 2 1100 50 60 hz L 24 528 IN 825000 SHEL LAB 9170598 X 063 SIGELDON MANLIACTURINO CORNELIUS OREDON MATERIAL REVISION n HYPOXIA 120V SCALE 1 16 SHEET 10F1 20 Bactrox Operations Manual DIAGRAMS WIRING DIAGRAM BACTROX 9851358 BLACK 14G EMI FILTER 2800502 WHITE 14G BLACK 14G OTP CONTROL 1750500 230V 120V COM GREEN LIGHTED POWER SWITCH 7850570 4880550 CHAMBER FAN AIR PUMP WHITE 14 60W 120V 60W 120V BED 14 ORANGE 14G RED 14G gau TB5 6 1750973 TEMP DISPLAY CONNECTOR 1 13 TTB1 14 TB5 1 02 DISPLAY 1750974 CO2 DISPLAY 1750975 BLACK 146 02 SST SENSOR 6600532 2 IR SENSOR 8320510 SUPPLY 6750516 TEMPERATURE RED 18G BLACK 18G CS
23. and a constant vapor pressure is generated in the tank above the liquid level The is drawn off of the top as a gas It is normal for the high pressure gauge on your regulator to start out reading 800 to 1000 PSI with a full tank The same vapor pressure is maintained as long as any liquid is left in the tank The reading will drop from 800 to 500 PSI quickly and will stay there for most of the duration of the tank At the end of use the pressure will drop quickly to zero to indicate that the tank is completely empty When the last of the liquid has evaporated into gas then the pressure will drop rapidly as the gas is drawn off 5 3 3 NOTE Pressure from the regulator to the incubator should be set at 15 PSI Models with additional internal regulators have pressure reduction to 10 PSI to prevent overshoot 5 3 4 NOTICE Only medical grade CO should be used in your Bactrox Failure to do so may damage the unit and void your warranty NOTE Pressure from the regulator to the incubator should be set at 15 PSI An additional internal regulator further reduces the pressure to 10 PSI to prevent overshoot 5 4 5 5 5 6 Hose ports for the gas are provided on the upper left side of the unit The Bactrox chamber has five access ports on the left side of the unit See Diagrams Section page 33 INTERIOR PLUMBING for more details Install one end of the hose from the gas regulator of the gas tank to the port marked 2 PORT on the
24. and leaving condensate 7 INCUBATOR TEMPERATURE CONTROLLER SET OVER TEMPERATURE Under certain circumstances samples placed directly on the bottom shelf may become too hot and dry out Shelf spacers are provided to minimize this condition Three spacers are provided for the BACTROX Place these spacers on the bottom shelf Note the slots go to the front and back 7 1 Setting Incubator Controller The incubator temperature controller regulates the incubator temperature by use of Up Down buttons To enter set point mode on the control press either the Up or Down button one time The digital display will start to blink going from bright to dim While blinking the Digital Display is showing the set point To change the set point use the Up and Down buttons If the buttons are not pressed for five 5 seconds the display will stop blinking and will read the temperature of the unit Note that the SET OVER TERMPERATURE knob should be turned to its maximum position clockwise until the unit has stabilized at desired set Bactrox Operations Manual 9 7 2 7 3 7 4 7 5 7 6 7 7 7 8 point temperature Allow the incubator at least 24 hours to stabilize Then re calibrate the digital display to your reference thermometer follow the calibration instructions This incubator was calibrated at the factory at 37 C Setting Incubator Safety First set the control to the desired incubator temperature and then the INCUBATOR SAFETY to
25. ction and adjustments are made Set the C02 control setpoint to OFF To do this press and release the down arrow Button The display will flash SP and then a number While the number is flashing press the down arrow button and hold it down until the display is flashing OFF then release the button Then press the down arrow until the CO2 setpoint is OFF Muting Audible Alarms Powering the unit on prior to calibration may activate the built in alarm system To mute the alarm hold EITHER the up or down button until the mute indicator activates Bactrox Operations Manual 13 10 5 10 6 Initial Control Settings Each Bactrox comes preset from the factory at 37C and the O2 and CO set to off You should allow the incubator to heat up and stabilize for 24hrs the SET OVERTEMPERATURE control clockwise until it stops so that it does not activate during the initial warm up Calibrating the Controls for Temperature or 10 6 1 Push and hold BOTH the UP and DOWN buttons simultaneously for five 5 seconds 10 6 2 If you are calibrating temperature the Temperature Display panel will read CO and then blink 10 6 3 If you are calibrating CO Level the will read CO and then blink 10 6 4 While the display is blinking use the UP and DOWN buttons to adjust the display to match the actual condition in the incubator chamber 10 6 5 If no buttons are pressed within five 5 seconds the blinking will stop and the
26. cubator Figure 5 Pass Box The PASS BOX provides ability to take material into and out of the chamber PASS BOX can be operated either automatically or manually The PASS BOX shelf allows for materials to be conveniently rolled from the PASS BOX into the chamber pe Figure 6 Incubator Bottom Shelf Spacer Three 3 are provided with the Bactrox Refer to Section 8 for installation and precautions Bactrox Operations Manual 5 2 RECEIVING AND INSPECTION 2 1 2 2 2 3 The carrier when accepting shipment also accepts responsibility for safe delivery and is liable for loss or damage claims On delivery you must inspect for visible exterior damage Note and describe on the freight bill any damage found and enter your claim on the form the carrier supplies Inspect for concealed loss or damage on the unit itself both interior and exterior If any the carrier will arrange for official inspection to substantiate your claim Save the shipping crate until you are sure the unit has been delivered in good condition If for any reason you must return the unit contact your sales representative for authorization and supply nameplate data 3 INSTALLATION 3 1 3 2 3 3 3 4 3 5 3 6 3 7 3 8 Local city county or other ordinances may govern the use of this equipment If you have any questions about local requirements please contact the appropriate local agency Under normal circumstances these units are inten
27. ded for use indoors at room temperatures between 5 and 40 C at no greater than 80 relative Humidity at 25 C and with a supply voltage that does not vary by more than 10 These chambers should not be operated at an altitude exceeding 2000 meters Installation category is Pollution Degree 2 Customer service should be contacted for operating conditions outside of these limits Installation may be performed by the end user It is unnecessary for this unit to be installed by a technician Location In selecting a location consider all conditions that might affect performance such as heat from radiators ovens autoclaves etc Avoid direct sun UV light sources fast moving air currents heating and cooling ducts and high traffic areas Allow a minimum of 10 cm between the unit and any walls or partitions that might obstruct free airflow and to promote easy access to the power cord and source Under normal circumstances this unit is intended for use indoors Power Source The unit power requirements are listed on the data plate Check the data plate for voltage cycle and ampere requirements Plug the power cord into a PROPERLY GROUNDED earthed Power source OF THE CORRECT SIZE AND STYLE INDICATED ON THE UNIT NAMEPLATE RATING A separate circuit is recommended for this unit to prevent loss of product due to overloading or circuit failures caused by other equipment The supply voltage must match the nameplate voltage within 10
28. f O2 inside the chamber workspace after initialization from ambient air 11 10 The CO2 control module will automatically add Carbon Dioxide gas or Nitrogen to achieve the desired setting Normally it takes 45 minutes to achieve 1 concentration of CO2 inside the chamber workspace after initialization from ambient air 11 11 If a precise control of the O2 concentration is required refer to the calibration section of this manual to enter a custom calibration The module is calibrated at the factory for two point curve at 1 and 20 7 Summary The chamber is ready when The SET OXYGEN led display reads the desired concentration of oxygen The SET CO 2 led displays reads the desired concentration of oxygen The SET TEMPERATURE controller displays the desired value NOTE It may take several hours to overnight to achieve temperature stability after the incubator doors are closed after initialization 12 PASS BOX OPERATION 12 1 12 2 12 3 Load the PASS BOX with samples materials supplies etc Close the outer and inner PASS BOX Doors Activate the PASS BOX Bactrox Operations Manual 14 12 3 1 Locate the START PASS BOX PURGE switch which should be in the bottom OFF position NOTE Do not hold Auto Cycle Start Switch down Simply press the button and let go to activate 12 3 2 Press the START PASS BOX PURGE switch once to initiate the cycle The PASS BOX will purge nitrogen thru the
29. imes a user enters their chamber daily operating technique and the number of PASS BOX purge cycles Our product literature states the PASS BOX uses approximately 45 seconds of gas to purge the oxygen to appropriate hypoxic levels Methods to Conserve Gas Users can utilize the PASS BOX as a holding station As samples are received they can be stored in a hypoxic environment by placing them in the PASS BOX and activating either the automatic or manual PASS BOX cycle 1 Users should evaluate and try to limit the number of times the PASS BOX is cycled For example placing samples in the PASS BOX once in the morning and once in the afternoon as opposed to four times a day is desirable 2 Gas is also conserved by bringing samples in through the sleeve System Easy samples to transport through the sleeves are transport tubes as well as plates that are sealed 3 Good sleeve system technique When a user is in the Working Area they should be aware of the effects their movements have on gas consumption Gas consumption can be reduced by balancing arm movements For example when extending the right arm into the chamber bring the left arm close to the front of the chamber thus balancing the pressure and reducing gas displaced by area Aborting PASS BOX PURGE CYCLE The automatic PASS BOX can be aborted by pressing and holding the START PASS BOX PURGE button for five 5 seconds If a user forgets to place a sample in the
30. in LC2 value of certified calibration gas in uppercase in the TERATERM program The display will show LC2 value of certified calibration gas 9 5 18 Unhook the calibration gas and reconnect the Calibration pump box again and allow at least 5 minutes for the sensor to stabilize This will show the curve is still accurate over the full range 9 5 19 The display should return to 20 7 percent Remove the tubing from the calibration port barb Replace the barb plug cap 9 5 20 Remove calibration cover Two point calibrations are now complete 9 5 21 Set the main SET OXYGEN 90 display to the desired setpoint via the up and down arrows Allow the reading to stabilize before use OFFSET CALIBRATION PROCEDURE 9 6 1 The second method of calibration does not require a computer It requires a calibration gas selected to match the process the chamber is going to be used Offset calibration is a single point calibration and should only be used in applications where having an absolute linear curve throughout the 2 range is not an issue Users should obtain calibration gas at a concentration that matches the value where the chamber is going to be operated 9 6 2 To calibrate install the calibration plate FIGURE 1 9 6 3 Hookup the calibration gas to calibration port FIGURE 2 9 6 4 Turn the calibration gas on with the regulator set no more than 3 PSI 9 6 5 Allow the control to stabilize for at least 5 minutes 9 6 6 If display does no
31. indicators for high and low alarm conditions that are activated whenever the actual O2 is 1 above or below the set point CO Alarms The controller has alarm indicators for high and low alarm conditions that are activated whenever the actual CO2 is 1 above or below the set point Audible Alarms Both controls have audible alarms that are activated when either of the HIGH or LOW indicators is activated Muting Audible Alarms The audible alarms can be muted for a single alarm occurrence by pressing and holding down EITHER the UP or DOWN button for several seconds until the alarm mutes There is a built in delay of approximately 15 minutes on the occurrence of a LOW alarm This time delay prevents the audible alarm from activating every time the door is opened and the temperature and drops 8 CO CONTROL CALIBRATION 8 1 8 2 8 3 Manufacturers Recommendation for Monitoring Levels sensors are factory calibrated and under normal circumstances need no calibration It is recommended that the accuracy of your CO control system be monitored by measuring the actual concentration on a weekly basis with a Fyrite or other measuring device This should be done when the chamber has not been opened for an extended period of time i e after the weekend and should only take 1 2 hours Setting the CO Control Attach the supply hose from the COetank to the Bactrox inlet fitting and turn on the
32. it prior to use Your operating conditions and appropriate protocol will determine the correct procedure for cleaning A typical cleaning procedure is described below and will help reduce the likelihood of contamination and the necessity of decontamination Clean the unit regularly Depending on usage and protocol this may be weekly monthly or quarterly Cleaning the unit does not qualify the unit as decontaminated Bactrox Operations Manual 6 WARNING Follow these safety precautions regardless of the cleaning procedure Failure to do so may cause serious injury or death Always disconnect the unit from the electrical service when cleaning Assure all volatile or flammable cleaners are evaporated and dry before reconnecting the unit to the power supply NOTICE Follow these precautions regardless of cleaning procedure Failure to do so may cause property damage and void your warranty Special care should be taken when cleaning around sensing heads to prevent damage The Bactrox unit was cleaned at the factory however a general cleaning is recommended Use BENZALKONIUM CHLORIDE to clean your chamber See list of chamber accessories products Do not use chlorine based bleaches ammonium based disinfectants or aerosol canisters or abrasive cleaners These will modify the powder coated and stainless steel interior finishes and create a biologically toxic atmosphere inside the chamber Do not use hard tools such as metal wire brushes or
33. its maximum position Allow 24 hours for stabilization before proceeding If after 24 hours the temperature is not at desired level adjust the SET TEMPERATURE up or down using the arrows until the precise desired temperature is achieved When stabilization at the desired temperature has been achieved turn the SET OVER TERMPERATURE control knob counter clockwise until the OVER TEMP ACTIVATED light comes on and audible alarm is activated Next carefully turn the SET OVER TERMPERATURE knob clockwise until the light is just off The safety is now set the OVER TEMP ACTIVATED light should remain OFF during normal operation NOTE An accurate thermometer should be used inside the incubator as a reference when setting and calibrating the incubator temperature It is a good idea to mark the SET OVER TERMPERATURE label with the desired setting or position as a backup in case the knob is moved accidentally If the OVER TEMP ACTIVATED light is on and audio alarm is activated at any time check the SET TEMPERATURE controller setting to be sure that it is not set above the SET OVER TERMPERATURE setting Alarm Controls and Display The temperature control O2 and the CO control are both equipped with visual and audible alarms Temperature Alarms The temperature control has alarm indicators for high and low conditions that are activated whenever the actual temperature is 1 C above or below the set point Oe Alarms The controller has alarm
34. kit The oxygen concentration in the chamber will fluctuate during the calibration procedure Sensitive items should be removed or placed in sealed containers if varying levels of Oz will have a negative impact Turn on the Bactrox and allow 5 minutes for the oxygen sensor to warm up The oxygen sensor needs to warm up before it can start reading oxygen levels accurately Turn on the computer and open the TERATERM software and click the serial button in the pop up window and click OK The controller should start logging once every minute If it does not log close TERATERM and reopen it again but before clicking okay try another com port Once the control starts to log into TERATERM communication has been established If you would like to save the oxygen readings to a log file Click on the File tab Then the Log tab Then create a filename to save your data in Click OK Data will now be saved into that file as reading is taken Plug in the Oxygen Calibration Pump into an electrical outlet and press the power button to turn on the pump Hook up the hose to the calibration port on the left hand corner of the unit Set the O2 set point to OFF Push the Set Oxygen down arrow and release When the green Oxygen display starts flashing push and hold down the down arrow until the display reads OFF Let the control stabilize at atmosphere for at least 5 minutes Before a calibration can be completed you must type BRO2 0 Setting thi
35. le and align top of fluid with zero on the scale Tighten screw Connect hose and aspirator bulb to unit being tested The sample port for connection is located on the control panel Place the hose sampling cap directly over the plunger valve on top of the canister and depress firmly With button depressed squeeze bulb 20 times On the last squeeze and with bulb still deflated release hose from button Turn Fyrite canister upside down 3 times each time allowing all fluid to flow to the opposite end of the canister Tilt canister to the 45 degree position to ensure all fluid has been released from top of canister Return canister to the upright position Read concentration WARNING The fluid used inside this fyrite instrument is poisonous and corrosive If taken internally could result in serious injury or death The fyrite indicator will come with a complete set of detailed instructions which should be followed carefully In the event of a spill or accidental body contact with the fyrite fluid follow the instructions on the refill bottle carefully 9 OXYGEN CONTROL CALIBRATION 9 1 9 2 9 3 9 4 There are two recommended methods of calibration depending on equipment availability and the accuracy required over a wide range of percent Oxygen Calibration is done using room air 20 7 is an average value based on the humidity and partial pressure of the atmosphere This value should be
36. ntrol has been activated and is actively injecting gas via the solenoid valve PASS BOX CONTROL The START PASS BOX PURGE button when engaged will actively initiate a PASS BOX purging cycle The cycle time is 45 seconds PASS BOX PURGING This pilot light is on whenever the PASS BOX PURGE button has been pressed The light will blink for the duration of the 45 second cycle RS232 Electrical connection for data logging and or calibration of the Oxygen Carbon Dioxide and Temperature controls 5 HOSE AND ELECTRICAL CONNECTIONS 5 1 5 2 5 3 A supply hose connects from the 2 PORT fitting to a N2 tank and regulator CGA 580 A supply hose connects from the CO2 PORT fitting to tank and regulator CGA 320 and CO Regulator Considerations 5 3 1 Ensure that you are using dual stage regulator CGA 580 for and CGA 320 for It is highly recommended that a good quality 0 60 PSI output range DUAL STAGE pressure regulator be used on the tank and tanks The dual stage regulator will have two pressure gauges The high pressure gauge 0 4000 PSI will indicate the pressure within the tank The low pressure gauge 0 60 PSI will indicate the output pressure on the supply hose to the chamber Single stage regulators do not provide as stable performance as dual stage Bactrox Operations Manual 8 5 3 2 Reading amp Understanding Your CO Tank Levels Pure CO is in a liquid state in the tank
37. ole of Condensate Controller The Condensate Controller is an exclusive Bactrox feature of Sheldon Manufacturing Inc Our competitors use desiccants Desiccants are a drying agent and often dry out samples Bactrox Operations Manual 17 Possible Cause Solution Amount of Water Collected in the The amount of water collected in Dehumidifier the dehumidifier depends on the number and types of samples in the chamber The typical amount collected is around 50 cc each day of chamber use Excessive moisture buildup in A Thermoelectric condensate Replace fan chamber controller fan is not operating B Ambient room temperature 1 Maintain room temperature a too low bit higher B2 Cover chamber with a blanket overnight as means of installation C Air conditioner unit directly C Relocate chamber redirect above chamber the air conditioning duct D Thermoelectric condensate 0 Empty thermoelectric controller not emptied condensate controller regularly container Bactrox Operations Manual 18 18 BACTROX OPERATION TIPS Gas Source Sheldon Manufacturing Inc recommends an two separate tanks of 100 2 and 100 Nitrogen for Hypoxic applications These mixtures are available marked NON FLAMMABLE Two national sources of gas are AIRGAS PRAXAIR and Air Liquid How much gas will a Bactrox chamber use Gas usage is dependent upon three things the number of t
38. s value to 0 will allow the controller to be accessed and calibrated Before a two point calibration is performed the offset calibration must be set to 0 To view the current offset type OC2 and press enter To set the offset calibration to zero subtract the offset value from the current display reading if it is a positive number then type in OC2 the result of display offset If the offset calibration value is a negative number then add the absolute value back to the current display reading OC2 the result of display offset Check that the Offset value is truly zero by typing OC2 and enter If the readout then displays OC2 0 then proceed to the next step If the display does not read 20 7 percent oxygen concentration then an upper calibration should be entered To enter an upper calibration type in UC2 20 7 in uppercase in the TERATERM program The TeraTerm window will display UC02 20 7 The digital SET 02 display should now read 20 7 percent oxygen Remove the Calibration pump tubing from the calibration port Hook up the certified calibration gas to the calibration port and turn the gas on with the regulator set no higher than 3 PSI Allow the control and sensor to stabilize for at least 5 minutes If the display does not match to what the certified calibration gas states then a low calibration needs to be entered Bactrox Operations Manual 12 10 9 6 9 5 17 To enter a low calibration type
39. seal the Arm ports while the chamber is not in use Doors can be stored using the holders provided while the chamber is in use Assembly consists of an Arm port door tightening knob and locking bar Doors are secured by turning the locking bars horizontally to pass through the cutouts in chamber then turning locking bars vertical and tightening the knobs Before leaving the chamber the incubator doors and the inner PASS BOX door must be closed Hold the locking bars in a horizontal position and close the doors Rotate the locking bars to a vertical position and gently tighten the black wing nut on both arm port door knobs just until they grab When the arm port doors are properly sealed slowly and carefully withdraw both arms from the sleeves 15 MAINTENANCE 15 1 15 2 15 3 15 4 15 5 15 6 15 7 DAILY ROUTINELY Before using any cleaning or decontamination method except those recommended by the manufacturer users should check with the manufacturer that the proposed method will not damage the equipment Remove and or empty condensate collection container Disinfect the chamber interior Do not use any volatile cleaning compounds such as alcohol bleach or volatile ammonium based cleaners as these compounds will create a toxic environment for the growth of the microorganisms Do not use alcohol based cleaning products on the Plexiglas Check the cuffs on the sleeve system If the cuffs have holes or any tears replace
40. steel wool Use non abrasive cleaners and soft tools such as plastic brushes Typical Bactrox Cleaning Procedure Remove the condensate collection vessel pan every week wash with soap and water and then disinfect with 70 alcohol solution Replace in the chamber Remove all shelves shelf supports shelf standards and shields Wash and disinfect as described in item 1 For initial set up clean and disinfect shelving parts prior to installation Wash and disinfect all interior surfaces including inside the incubator Replace all N2 and CO2 filters every six months or when noticeably dirty on the upstream side CO2 and N2 filters are located in the shadow box just behind the GAS IN fitting and in line with the 2 tubing kit and inside the pump box in the shadow box 3 9 Gas Source Install the gas regulator s on the tank s of gas Chain the gas tanks s to a secure position on the wall Set the regulator s to 15 psi 3 10 achieve a hypoxic 1 20 Oz environment we recommend using a tank of pure Nitrogen 3 41 achieve a hypoxic atmosphere enriched with carbon dioxide 1 20 we recommend tank of pure Carbon Dioxide CO 3 12 2 must be connected to N2 PORT 2 must be connected to the CO2 Port 8 oz 2z 55 02 CAL PORT Figure 7 Bactrox Operations Manual 7 4 CONTROL PANEL OVERVIEW 4 1 4 2 4 3 4 4 4 5 4 6 4 7 4 8 4 9 4 10 4 11 4 12 4 13
41. t match what the certified calibration gas states then an offset calibration needs to be entered 9 6 7 Toenter the offset calibration push and hold both up and down arrow simultaneously until CO flashes and then the current display value flashes then release the buttons 9 6 8 While the current display value is flashing use the up or down arrow to change the value in the display to match the calibration gas 9 6 9 After the value is entered the display will stop flashing after 5 seconds if no buttons are pushed returning to normal operation The display should now match the calibration gas 9 6 10 Remove the calibration gas and calibration cover It is important to note that a single point calibration may cause readings outside of the calibration gas to be incorrect POWER ON THE BACTROX 10 1 10 2 10 3 10 4 Check power supply against unit plate and make sure they match Be certain that the fuse is installed in the power inlet of the unit Start by attaching the cordset supplied with the accessories to the unit inlet and the building power supply Attach the female end of the cordset into the fused and recessed male plug on the rear of the unit and the male plug end of the cordset into the building supply receptacle Check that the appropriate plumbing connections have been made for N2 and CO2 supply Turn the power switch to the I ON position Operating temperature must be set achieved and calibrated before C02 conne
42. tional accessory used in a two gas chamber Part 7150500 Plexiglas Cleaner 1060503 8 oz size Plexiglas Scratch Remover 1060504 8 oz size ACCESSORIES PARTS LIST Catalog No Product 5110729 Petri Plate Rack 2x13 Sleeves Complete 9990738S Small 9990738M Medium 9990738L Large 9490507 UV Light Source 9490512 Start Up Kit 3600500 Rubber Cuffs Sm Size 7 3600501 Rubber Cuffs Med Size 8 3600502 Rubber Cuffs Lrg Size 9 6000501 O Ring For Door 6000504 O Ring For Cuff 3450506 Gasket Airlock 3600513 Nitrile Gloves Size 7 3600514 Nitrile Gloves Size 8 3600515 Nitrile Gloves Size 9 3600525 Nitrile Cuffs Size 7 3600526 Nitrile Cuffs Size 8 3600527 Nitrile Cuffs Size 9 7200502 Rubber Cuff Plugs Bactrox Operations Manual 16 CAUTION Extreme caution must be exercised any time access is made into areas housing electrical components Repair replacement or adjustment of components in these areas must only be done by qualified technicians familiar with electrical circuitry and the operation of the hypoxic chamber ATTENTION Des Pr cautions extremes sont requises a chaque fois que vous acc dez a des endroits qui abritent les composantes lectriques La r paration le remplacement oi iajustement de composanted dans ces endroits ne doivent tre effectu s que par un technicien qualifi familier avec les circuits lectriques et op ration des tuves ana robiques 17 TROUBLESHOOTING I PASS BOX Problem Possible Cause Sol
43. ution PASS BOX Purge will not start A Input output terminal buss is not A Press down the terminal buss seated correctly so that it snaps into place NOTE Power should be turned off B User pushed start switch and B Push start switch once then held inadvertently held down release Thus aborting Auto Cycle D Start button inoperative D Replace start button E No power to controller check E1 Check for 24 volt DC out of power light on Controller power supply E2 Check for continuity between power supply and controller ll INCUBATOR Problem Possible Cause Solution Steady digital readout matches Temperature Controller out of A Follow Complete Temperature set point but actual temperature calibration Controller instruction does not B Incubator door open B Close incubator door and allow incubator to stabilize Digital readout actualjA Output relay stuck Check to A Replace Temperature temperature continue to increase see if Incubator Heating Controller past set point Indicator light stays on while temperature is Increasing B Incubator door open B Close incubator door and allow incubator to stabilize Audible alarm sounds A Temperature has risen beyond Determine cause of the set range for the High temperature increase from Limit Safety prior section and take appropriate action Call for service MISCELLANEOUS Possible Cause R
Download Pdf Manuals
Related Search