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Introduction to the Agilent 218 Solvent Delivery Module

1. For the Agilent 1200 Infinity Series LC systems the pressure module must go into the topmost pump Purification Solution System User Guide 43 3 Installation If you are using a computer as controller the pump with the pressure module needs to be identified in the controller configuration window 1 Decide which pump is to have the pressure module 2 Place the pressure module in position and attach the installed i e which pump is the master pump then connector to the plug on the inside of the pressure remove the panel on the rear of the right side using a module compartment The connection is done using a Phillips head screwdriver 9 pin D shell connector The 4 pin side of the connector is positioned towards the rear of the pump module The module and its connector are a one way fit Mixer connection with flat side toward rear of connector D shell connector with 4 pin side toward back of module ra SE w PRESSURE MODULE 8700psi 10 mL Pressure module connector Keep the panel in a safe place Next Steps 3 Tighten the screws on the mounting panel to hold the module in place This is critical as the pressure module can slip out if the pump is picked up without the screws being tightened 44 Purification Solution System User Guide Installation 3 Internal Mixer Installation If two or more pumps are being used together to proportion individual solvents into
2. gt Do not store ship or use your module under conditions where temperature fluctuations could cause condensation within the module gt If your module was shipped in cold weather leave it in its box and allow it to warm slowly to room temperature to avoid condensation Area selected WARNING Explosion damage and accuracy of the module gt Select an area free from drafts corrosive atmospheres and vibration gt Select a dust free low humidity environment gt Use air conditioning for control of the environment Bench Space Make sure that the bench is designed to bear the weight of all modules For details on the space needed around the individual modules refer to the according manuals e Agilent 218 Solvent Delivery Module User Manual G9300 90001 Agilent 325 UV VIS Dual Wavelength Detector User Manual G9309 90000 Agilent 410 Autosampler User Manual G9331 90000 e Agilent 440 Fraction Collector User Manual G9340 90000 20 Purification Solution System User Guide Site Requirements and Specifications 2 Specifications For details on specifications of the individual modules please refer to the Agilent 1200 Infinity Series Specifications Compendium or to the corresponding User Manuals Purification Solution System User Guide 21 2 Site Requirements and Specifications Specifications 22 Purification Solution System User Guide Purification Solution System User Guide 3 Installation
3. Create Location k Instruments Application Fj Instruments X Administration Your startup license expires in 60 days 2 Define the Name Example PrepLC 116 Purification Solution System User Guide Appicaton instrument controler instrument type Contact 0G Wstruments SK Administration Your startup license expires in 60 days 4 Select Configure Instrument z Hees o IROA EARR Edit Delete Refresh Lock Create Configure Edit Notifications Shortcuts Instrument Instruments and Locations Properties Actions Navigation PrepLc Not Connected EEEE Inst s Start Instrument J PreplC a Launch o Launch Offiine Status Ei instruments X Administration Your startup license expires in 60 days Purification Solution System User Guide 3 117 3 Installation 5 Select No to avoid the auto configuration It doesn t work with this version store Agilent LC System Z 20 wec evehaton J Enable reeigert Repotng f Empty configuration Low Plow Sampler euto configure a worm BR o Amo Ya Aak tor configuration change at ChemStaton statue f 2 Low Plow HP Sampler i There is ne configuration available for this instrument De you want to i x Cancel Hep 6 The left panel of the Configurable Modules contains the Agilent Prep LC Ortens Agilent LC System Use classe devers Ti 2D spectra evatuaton 7 Enable rtebgert Rrpoting
4. Delivery 26 Components of a Complete System 26 Damaged Packaging 28 Check Delivery 29 Delivery Checklists 29 Unpacking and Inspection 32 Optimizing the Stack Configuration 35 Isocratic System 36 Binary System 37 Auto Preparative System 38 Installing the Solvent Delivery Module 39 Electrical Setup 39 Pump Head Installation 41 Pressure Module Installation 43 Internal Mixer Installation 45 Mast Kit and Components Installation 47 Installing the Mast 48 Installing the Manual Injection Valve and Bracket 49 Installing the Prime Purge Valve and Bracket_system 51 Installing the 3 way Pump Head Prime Valve 52 Installing the Column Hanger 54 Plumbing Connections 55 Setting the Pump ID and Pump Head Size When Using HPLC Control Software 69 Setting the Pump ID and Pump Head Size When Using an Agilent 218 Pump as a Master Controller 70 Ee Agilent Technologies 23 3 24 Installing the Detector 71 Location of the Detector Module 71 Power Connection and Rear Panel Services 72 Avoiding Harmful interferences to Radio or Television Reception 74 Removing the Front Panel 75 Hydraulic Connections Flowcells 76 Installing a Flowcell 77 Detector Outlet Back Pressure Restrictor 81 Installing the Door 82 Installing and Removing the Door 83 Installing the Autosampler 84 Location of the Autosampler Module 84 Overview of the Module 85 Installing the Sample Tray 88 Connecting the Waste Tubings 88 Starting the Autosampler 90 R
5. 1 Do not connect to Chassis Gnd Installing the Capillary Kits 1 Install the Capillary Kit as described in the Technical Note of the Binary or Isocratic Pump Stainless Steel Tubing Kit Setting the Pump ID and Pump Head Size When Using HPLC Control Software This is done on the pumps whether you are using an HPLC control software or using one of the pumps as a master controller To set the pump ID when all pumps are slaves 1 Turn the pump on 2 Press SETUP and then press ID 3 Set the ID for the Agilent 218 Pump either by entering a number between 0 and 63 or pressing the UP ARROW or DOWN ARROW key to scroll through a preset list of choices Available choices are 0 63 MC master controller or no ID To set the pump head size when all pumps are slaves 1 Turn the pump on 2 Press SETUP and then press HdSz Purification Solution System User Guide 69 3 70 Installation 3 Use the UP ARROW or DOWN ARROW key to select between a preset list of choices Choices are 5 10 25 50 100 and 200 mL min 10P 25P 50P 100P The P designation stands for PEEK The compressibility compensation for PEEK heads is different than for stainless steel or titanium heads Setting the Pump ID and Pump Head Size When Using an Agilent 218 Pump as a Master Controller Setting the pump ID and pump head size 1 Turn the pump on 2 Press SETUP 3 Press PUMP 4 Select between A B C and D Selecting a pump opens a w
6. 3 Installation Setup the Fraction Collector The Fraction Collector is ready to be set up in OpenLAB and does not need to have the hardware set up 114 Purification Solution System User Guide Installation 3 Setup the Software Install Agilent OpenLAB CDS ChemStation PrepLC Drivers Parts required p n Description M8301AA OpenLAB CDS ChemStation Edition Workstation A 01 05 or higher M8500AA M8500AA LC driver M8366 64000 OpenLAB CDS Installation Driver Prep LC M8367 64000 OpenLAB CDS Installation Upgrade Driver Prep LC Hardware required Computer with OpenLAB CDS ChemStation Edition installed For details refer to the OpenLAB WorkStation Installation Guide 1 Copy AgilentDriversPrepLCChemstationSet_up msi from CD to your computer 2 Doubleclick on AgilentDriversPrepLCChemstationSet_up msi and follow the steps in the setup wizard Purification Solution System User Guide 115 3 Installation Setup the System with OpenLAB CDS ChemStation Edition Control Panel Preparations Latest driver package installed see OpenLAB CDS Installation Driver Prep LC M8366 64000 Modules are wired as described in Cable Connections on page 139 All modules are setup as described in Setup the Hardware on page 110 All modules are switched on OpenLAB configuration 1 Open the T OpenLAB Control Panel and select Create gt Create Instrument QR ii Create Refresh Edit Nosikations Columns Create Instrument s Properties
7. Contgurabie Mosses EPs Sten zinc pi sree rasa Aak for confiqueition change at ChamStaton startup Mistiri configuration x Corcel Hep 7 Select the module you have available in your system 8 Click to add the modules to the Selected Modules The configuration dialog for the selected module opens 118 Purification Solution System User Guide Installation Pump Configuration 1 Doubleclick in Configurable Modules on Pump Pump Configuration dialog opens Select the COM Port as COM 1 and the desired Pressure unit bar available kPa PSI bar MPa Pump Configuration Instrument 1 COM Port coM1 x Number of pumps i Pressure unit bar Pal Injection pump present Pump ID Model Firmware Head version A 1 PrepStar218 HPXLV2 0SD300V1 1 25mL Titanium Activate communication i Communication established with COM1 i OK Canc Help 3 Verify the connection by clicking on Activate communication Information Communication established with COM1 is visible on the screen 4 Click OK Configuration dialog closes Purification Solution System User Guide 119 3 3 Installation Autosampler configuration 1 Doubleclick in Configurable Modules on Autosampler Autosampler Configuration dialog opens 2 Select the COM Port as COM 1 F D Autosampler Configuration Instrument 1 COM Port com x ID 60 l Activate communication
8. Filling and Sealing the Vials 1 Fill the standard vials as well as the conical vials by means of a narrow end pipette to allow air to escape when filling the vial Do not fill vials completely to the top In that case sample will be forced into the air needle risking cross contamination of samples and fouling of the needle pair 2 Check that the seal is airtight due to maintain a pressure on the vial for air bubble prevention and to prevent evaporation of volatile samples 3 Check seal after crimping if the cap can be turned easily the seal is not airtight re adjust hand crimper When using uncapped vials the performance of the AutoSampler may not meet the specifications precision Do not re use a sample vial without replacing its cap or septum Purification Solution System User Guide Installation 3 Loading the Sample Tray 1 Place the vial in the tray and link them to methods in the Series For details see Agilent 410 Autosampler User Manual G9331 90000 Chapter Using section Programming the Run Sequence or Series 2 Ifthe autosampler ist not running manually rotate the tray to gain access to all vial positions Purification Solution System User Guide 93 3 94 Installation Installing the Agilent 410 Autosampler Prep Option 1 ao ol A Ww Replace the standard injection valve with the special Agilent 410 Prep valve Replace standard sample needle air needle and buffer tubing with the ones suppli
9. 160 Bij dit apparaat zijn batterijen geleverd Wanneer deze leeg zijn moet u ze niet weggooien maar inleveren als KCA Purification Solution System User Guide Appendix 6 Radio Interference Cables supplied by Agilent Technologies are screened to provide optimized protection against radio interference All cables are in compliance with safety or EMC regulations Test and Measurement If test and measurement equipment is operated with unscreened cables or used for measurements on open set ups the user has to assure that under operating conditions the radio interference limits are still met within the premises Purification Solution System User Guide 161 Appendix Electromagnetic Compatibility 162 EN55011 CISPR11 Group 1 ISM equipment group 1 contains all ISM equipment in which there is intentionally generated and or used conductively coupled radio frequency energy which is necessary for the internal functioning of the equipment itself Class A equipment is equipment suitable for use in all establishments other than domestic and those directly connected to a low voltage power supply network which supplies buildings used for domestic purposes This device complies with the requirements of CISPR11 Group 1 Class A as radiation professional equipment Therefore there may be potential difficulties in ensuring electromagnetic compatibility in other environments due to conducted as well as radiated disturbances Opera
10. 200 mL min head only 1 Remove the shipping plug from the liquid head aperture on the front of the pump 2 Insert the pump head into the front aperture of the pump NOTE The notch at the bottom of the pump head body must fit the matching pin on the pump below the aperture This notch ensures that the inlet and outlet ports are located in the correct position 3 Holding the pump head in place slide the clamp found in the pump head kit down over the head so the clamp flanges engage the slots on both sides of the pump head Purification Solution System User Guide 41 3 42 Installation Depending on the position of the pump cam you may have to push the pump head in to get the clamp on Make sure that the clamp flanges are in their slots on both sides and finger tighten the thumbscrew until the clamp holds the pump head securely In the case of the 200 mL min head use the supplied hex wrench to tighten hex screw on the clamp Tighten very securely Repeat this procedure for each pump head in your system There are three sizes of pump head clamps The smaller one fits all pump heads that do not have piston wash The larger one fits all heads that have piston wash The largest clamp is used only with the 200 mL min head 4 Enter the pump head size into the pump software and into the pump firmware from the key pad For details see Setting the Pump ID and Pump Head Size When Using HPLC Control Software on page 69 or
11. 65 3 66 Installation Connecting Communication Cabling Using Serial Interface On the rear panel there is a single RS 422 male connector for serial communications Communications between slave pumps and controller a computer or master pump are established through the serial interface channel This bi directional communication protocol uses the EIA RS 422 RS 485 interface specification for data transmission Internal software in the pump determines whether the pump is a master controller or a slave pump One software controlled connector located on the back panel sends and receives signals from the controller master pump or external computer This connector is used on all pumps both when the pump is used as a controller or as a slave the software informs the controller of the status of each pump The various configurations are discussed below Connection with a computer is covered in the interface manual of the specific software system used Using OpenLAB as the System Controller 1 Cable your system see Cable Connections on page 139 2 Go to Setting the Pump ID and Pump Head Size When Using HPLC Control Software on page 69 or Setting the Pump ID and Pump Head Size When Using an Agilent 218 Pump as a Master Controller on page 70 to set the pump ID and pump head size Using a Agilent 218 Pump as a System Controller 1 Enter the ID number of each Agilent 218 Pump using its own keypad Each pump in the syst
12. Outlet check valve A Liquid head B Figure 8 High pressure tubing connections A titanium or stainless steel heads B PEEK Heads Installation of high pressure tubing for titanium or stainless steel heads 1 Thread one of the 14 28 male nuts and ferrules onto the outlet tubing For orientation see A 2 With the nut and ferrule in place insert the tubing into the outlet check valve as far as it will go 3 Holding the tubing in place tighten the nut 1 4 turn beyond finger tight with an open end wrench to swage the ferrule to the tubing 4 Repeat this process at the other end of the outlet tubing for the next device in line mixer pressure monitor etc If the connection leaks when the system is pressurized tighten the nut slightly until the leak stops 58 Purification Solution System User Guide Installation 3 Installation of high pressure tubing for high pressure tubing for PEEK heads 1 Thread one of the polyacetal nuts and ETFE ethylene tetrafluoroethylene 2 piece ferrules onto the outlet tubing For orientation see B 2 With the nut and ferrule in place insert the tubing into the outlet check valve as far as it will go 3 Holding the tubing in place tighten the nut 1 4 turn beyond finger tight with an open end wrench to swage the ferrule to the tubing 4 Repeat this process at the other end of the outlet tubing for the next device in line mixer pressure monitor or drain valve If t
13. slow blow gt All fuses must be UL listed and CSA certified or IEC 127 type 90 Keep the keyboard front cover closed during operation Remove the safety screw on the right hand side of the front cover Check fuses and voltage settings on the rear of the instrument Connect the power cable FP O N Turn the power switch to ON The Ready screen appears displaying the firmware revision number Purification Solution System User Guide Installation 3 Rinsing the System with Wash Solvent Tools required OR CAUTION Description Distilled Water lsopropanol Helium Ultrasonic bath Damage to the system Crystals from salts or buffer solutions may block or damage the system gt Never use salts or buffer solutions as wash solvents gt Only use water organic solvents 1 Place a clean bottle for the wash solvent at the left hand side of the autosampler 2 Use a mixture of distilled water and isopropanol 80 20 v v or the mobile phase as wash solvent Degas the wash solvent with Helium or an ultrasonic bath Put the wash solvent tubing in the filled was solvent bottle Press MAINTENANCE to go the autosampler maintenance functions ao oo A Ww Fill the tubing using the autosampler soft function keys SYR END and SYR HOME 7 With SYR END a syringe volume of wash solvent is aspirated from the wash solvent bottle and the wash solvent tubing is filled With SYR HOME the syringe contents will be
14. tighten very slightly with an open end wrench until the leak stops Do not over tighten The fitting may be damaged Installation of inlet tubing for 200 mL min heads 1 Remove the solvent inlet assembly from the accessory package and immerse diagonally cut end of tubing into solvent container The solvent containers should be located on the floor with the pump on the bench 2 Connect the inlet fitting to the inlet port on the pump head check valve the lower port Tighten the inlet tubing finger tight If leakage occurs in use tighten very slightly with an open end wrench until the leak stops Do not over tighten The fitting may be damaged Installing the Piston washing Tubing Skip this step if you do not have piston washing on your pump heads The wash tubing is provided as a single piece When pumps are stacked the tubing from the upper pump rinse outlet can be connected to the rinse inlet of the lower pump so that pump heads can be rinsed in series The tubing clamp need only be attached to the outlet rinse tubing on the bottom pump 1 Cut into two pieces of appropriate length for the wash inlet and outlet 2 Thread male Luer fittings into the inlet top and outlet rinse ports and connect the silicone tubing to the Luer fittings 3 Attach the tubing clamp to the outlet tubing Purification Solution System User Guide 57 3 Installation Pump Head High Pressure Tubing Tubing po Fitting To e Ferrule
15. 10 mL sample loop enables you to inject from microliters to milliliters with the same AutoSampler Flow rates up to 200 mL per minute are possible when in the Prep mode Table 1 Tubing of the Agilent 410 Autosampler prep option Tubing Material Dimensions Volume LSV sample needle Stainless Steel 70 mm x 0 81 mm o d x 0 51 mm i d 45 uL and tubing LSV sample needleto ETFE 155 mm x 1 16 o d x 0 50 mm i d 45 uL high pressure valve Buffer tubing from PTFE 2550 mm x 1 16 o d x 1 mm i d 2000 pL high pressure valve to syringe valve 14 If the Prep option is factory installed the installation instructions can be skipped If the Prep option is bought as a kit carry out the installation instructions see Installing the Agilent 410 Autosampler Prep Option on page 94 for more information Purification Solution System User Guide Introduction 1 Introduction to the Agilent 440 Fraction Collector The Agilent 440 Fraction Collector is a random access single probe fraction collector and can accommodate a variety of racks It is designed to automate the sample collection process This fraction collector is designed to meet the diverse requirements of high throughput laboratories Three racks are included with the fraction collector and are made of polypropylene to resist most chemical spills Up to three racks of many configurations can be placed on the fraction collector Additional sample racks can be set up in sequence a
16. Connect the OUT port on the stream splitter to the fraction collector oF Ww N Connect To Detector port in the back of stream splitter to the ELSD detector Purification Solution System User Guide 109 3 Installation Setup Hardware Setup the Hardware This is done on the modules regardless of whether you are using HPLC control software or using one of the pumps as a master controller including following modules Different pumps 325 UV VIS Dual WL Detector 440 Fraction Collector and e 410 Autosampler 110 Purification Solution System User Guide Installation 3 Setup Hardware Setup the Pump NOTE The correct setup of the pump depends on the pump head installed Choices are 5 10 25 50 100 and 200 mL min 10P 25P 50P 100P The P designation stands for PEEK The compressibility compensation for PEEK heads is different than for stainless steel or titanium heads Setup Pump ID for Pump 1 1 Turn the module on 2 Press on the Pump keyboard Setup and then Enter 3 With the left arrow key select ID of the Pump 1 HDSZ Pump head xx depends on what pump head is installed for choices see Note above Refill 125 piston CIM 5 4 Press Enter 5 Reboot the module Purification Solution System User Guide 111 3 Installation Setup Hardware Setup Pump ID for Pump 2 if Exist 1 Turn on the Pump 2 2 Press on the Pump keyboard Setup and then Enter 3 With the left arrow key sele
17. FW version Options Tray 84 standard vials Loop volume 100 pL Syringe volume Tubing volume Preparative option No V Column oven W Tray cooling v Alarm buzzer i Communication established with COM1 OK Coe e Select the Tray84 standard vials as an example Double check your actual configured Tray Check the other variables present in the Autosampler Configuration screen ao oo A Ww Verify the connection by clicking on Activate communication Information Communication established with COM1 is visible on the screen 7 Click OK Configuration dialog closes 120 Purification Solution System User Guide Installation 3 Detector Configuration 1 Doubleclick in Configurable Modules on Detector Detector Configuration dialog opens 2 Select the Browse function to start discovery your Detector The IP address will be populated by the searching results Example IP address 192 168 0 97 r r Detector Configuration Instrument 1 i Detector Configuration Instrument 1 os IP address 0 0 00 IP address 192 168 097 g Activate communication __ Actwate communication Dual wavelength option Enabled Dual wavelength option Enabled Celltype 20 Cel type a0 E Enable ready in Enable ready in i Discovering instruments i Communication established with 192 168 0 97 ok Cancel Hep ox Cancel Hep L
18. Purification Solution System User Guide Installation 3 Mast Kit and Components Installation Installing the Mast on page 48 Installing the Manual Injection Valve and Bracket on page 49 1 2 3 Installing the Prime Purge Valve and Bracket_system on page 51 4 Installing the 3 way Pump Head Prime Valve on page 52 5 Installing the Column Hanger on page 54 Purification Solution System User Guide 47 3 Installation Installing the Mast Parts required Description Mast kit 2x mast clamp bracket assembly 2x 10 32 socket scres stainless steel mast 1 Remove the mast brackets and two screws from the Mast Kit Assembly 2 Attach a mast clamp to the fittings at the front corner of the pump OR Attach a mast clamp to the fittings one each on the lower front corner of the pump and top front corner of the detector if fitted 3 Secure the stainless steel mast within the clamps so the lower end of the mast is level with the bench 48 Purification Solution System User Guide Installation Installing the Manual Injection Valve and Bracket Tools required Description Hex key 2 mm provided with the injection valve Parts required p n Description Injection valve R000048605 Injection valve bracket 3 1 Unpack the injection valve with attached black cable Selection valve grub screw Dust cover with _ needle port inside it Selction valve 2 Remove the red dust cove
19. Relay interface cable for relay interface board one relay contact per cable 110744200 Analog signal cable 138 Purification Solution System User Guide Cables 5 Cable Connections Cable Connections Back connectors for 218 to internal CIM 9 Pin connectors Out Gn Serial cable communication ee Converter RS 232 tR S Ethernet GPIB hat aes Next injection cable 1 218 Pump 410 Autosampler Le 3 325 Detector 440 Fraction le iT CPU Inject marker cable Figure 25 Cable connections for workstation control of Agilent 218 Pumps Agilent 325 Detector Agilent 410 Autosampler and Agilent 440 Fraction Collector Purification Solution System User Guide 139 5 Cables Analog Output Analog Output For analog output signals install the optional Analog signal cable 110744200 into the J14 receptacle Pin designations are shown below J14 2 Channel A 4 Channel B Figure 26 Pin designation for J14 The open ends of the analog output cable have labels with the signal names Channel A Channel A and Channel B Channel B 140 Purification Solution System User Guide Cables 5 Relay Output Relay Output For time programming external events a contact closure Relay output is available To configure the Relay output install the optional Assy PWB relay interface 325 210187590 into the J4 receptacle Pin designations ar
20. careful about possible ignition due to static electricity To prevent the build up of static electricity use a conductive container for waste gt Use only approved regulator and hose connectors refer to the supplier s instructions gt Keep solvents cool and properly labeled Ensure that you have the correct solvent before connecting it to the instrument Purification Solution System User Guide 153 Appendix WARNING Inflammation or injury due to toxic corrosive or stimulative solvent gt Do not contact toxic corrosive or stimulative solvent gt For details of the properties of each solvent and how to handle it refer to the relevant Material Safety Data Sheets MSDS gt Be sure to handle each solvent properly gt Wear proper personal protective clothes e g safety goggles so that a solvent will not come into direct contact with the skin gt Ventilate the laboratory room adequately to prevent accidental inhalation of harmful solvent vapor Cuts gt When working with glass or quartz parts take care to prevent breakage 154 Other Precautions Airflow to the cooling fans of the liquid chromatograph must be unobstructed Do not block the ventilation grills on the liquid chromatograph and accessories Consult the manuals supplied with your PC monitor and for their specific ventilation requirements Purification Solution System User Guide Appendix 6 High Pressure Hazards WARNI
21. interference received including interference that may cause undesired operation If this equipment does cause harmful interference to radio or television reception which can be determined by turning the equipment off and on the user is encouraged to try one or more of the following measures 1 Relocate the radio or antenna 2 Move the device away from the radio or television 3 Plug the device into a different electrical outlet so that the device and the radio or television are on separate electrical circuits 4 Make sure that all peripheral devices are also certified Make sure that appropriate cables are used to connect the device to peripheral equipment Purification Solution System User Guide Installation 3 6 Consult your equipment dealer Agilent Technologies or an experienced technician for assistance 7 Changes or modifications not expressly approved by Agilent Technologies could void the user s authority to operate the equipment Removing the Front Panel 1 If installed remove the door from the Agilent 325 Detector see Installing and Removing the Door on page 83 for more information 2 Remove the panel on the front right side of the detector by unscrewing the captive screw in the top left corner of the panel Figure 15 Removing the front panel Purification Solution System User Guide 75 3 Installation Hydraulic Connections Flowcells Hydraulic connections are located at the front of
22. into the rear panel connector 5 P Figure 28 Relay output board 142 Purification Solution System User Guide Cables 5 The Relay interface cable for relay interface board one relay contact per cable 110743800 is used to attach to the 3 pin connectors at the relay output board With the 3 pin connectors the contact closure is between pins 1 and 2 of the plugs Pin 3 is connected to ground The relay interface cable has three open ended wires The relay contact is connected between the clear and the black wire The green wire is connected to ground Purification Solution System User Guide 143 5 Cables Desktop PC Communications 144 Communication between the detector and a desktop PC occurs by an Ethernet connection Communication by an Ethernet connection is required to control the detector remotely by OpenLAB When the Workstation provides HPLC system control the synchronization cables from P9 and J10 are not used To create an Ethernet connection insert an RJ45 cable included in the ship kit into the J1 receptacle and into the PC The Ethernet cable that comes with the detector is a cross over cable which is appropriate for connecting the detector directly to a PC Connecting the detector to a network or a hub will usually require a patch cable A Ethernet cable for use in a network 392612901 can be purchased from Agilent or either locally Most PCs come pre configured with an Ethernet connection
23. one mixture a mixer is required Mixers are available in several different materials and three different sizes e A0O 6 mL 1 2 mL and 10 mL mixer The internal mixer is identical in function and capability to the external mixer except that it obtains power from the pump drive module and it mounts into the pressure module bay at the right rear of the pump The mixer is identified by a label on the inside of the mixer mounting panel This identifies the mixer size material and part number The mixer connects to the Agilent 218 Pump using a 2 pin Molex connector for power There is no ON OFF switch for the mixer Whenever the Agilent 218 Pump is powered on the mixer is running This continuous running does not hurt the mixer When no liquid is present the mixing bars may not be moving This is normal When liquid is present the mixing bars flip back and forth rapidly to mix the solvents 1 Ensure the pump power is turned off 2 Remove the cover panel from the right rear of the Agilent 218 Pump Save the screws Purification Solution System User Guide 45 3 Installation Installing the Solvent Delivery Module 3 Attach the 2 pin Molex connector to the white Molex 4 Fit the mixer panel onto the module and use the screws connector in the mixer compartment The flat sides of the to fasten it to the pump connector should be toward the rear of the pump Figure 6 Mixer internal connections 46
24. output can be used to activate Enable In on the next instrument When Enable Ready In is set software switch this polarized input signal must be present before the Agilent 325 Detector can go to the Ready state Specifically when the Agilent 325 Detector is in the NOT Ready Lamp On state on receiving a Ready In signal a monitor period will occur after which the Agilent 325 Detector goes to the Ready state It must stay active until the Agilent 325 Detector starts Ready In will be ignored in all other states This polarized output signal indicates that the Agilent 325 Detector is in the Ready state and is ready to start a time program This polarized edge triggered input signal will start the active method if the Agilent 325 Detector is in the Ready state This polarized output signal will be activated for 600 ms when the Agilent 325 Detector starts a time program This polarized edge triggered input signal informs the Agilent 325 Detector that a fault condition exists in another instrument in the system The Agilent 325 Detector halts the time program and sends a Fault Out signal The lamp can be programmed to either remain on or turn off upon receiving a fault signal This polarized output signal will activate for 600 ms when either of the following conditions occurs The Agilent 325 Detector discovers an internal fault condition that warrants aborting the run The Agilent 325 Detector receives a Fault In signal and it has no
25. starting with 13 August 2005 This product complies with the WEEE Directive 2002 96 EC marking requirements The affixed label indicates that you must not discard this electrical electronic product in domestic household waste Product Category With reference to the equipment types in the WEEE Directive Annex I this product is classed as a Monitoring and Control Instrumentation product Do not dispose off in domestic household waste To return unwanted products contact your local Agilent office or see www agilent com for more information Purification Solution System User Guide 159 6 Appendix Batteries Information Lithium batteries may not be disposed off into the domestic waste Transportation of discharged Lithium batteries through carriers regulated by IATA ICAO ADR RID IMDG is not allowed Danger of explosion if battery is incorrectly replaced gt Discharged Lithium batteries shall be disposed off locally according to national waste disposal regulations for batteries gt Replace only with the same or equivalent type recommended by the equipment manufacturer Lithiumbatteri Eksplosionsfare ved fejlagtig handtering Udskiftning ma kun ske med batteri af samme fabrikat og type gt Lever det brugte batteri tilbage til leverand ren Lithiumbatteri Eksplosionsfare Ved udskiftning benyttes kun batteri som anbefalt av apparatfabrikanten gt Brukt batteri returneres appararleverandoren
26. the solvent reservoir and to the rest of the system They also have individual nuts bushings and ferrules that are used to connect the pump to the rest of the system Use those parts to make the connections gt If you want to run the pump without liquid flow remove the liquid head or use demo mode Purification Solution System User Guide 55 3 56 Installation Pump Head Low Pressure Tubing and Inlet Filter Assembly The four types of inlet filter assemblies are shown below 10 mL min Heads u H hmr Install filter on tubing To pump inlet 25 mL min Head Tefzel fitting translucent Delrin fitting white A itr T inlet Install filter on tubing N A Flangeless fitting amp ferrule 100 mL min Heads Delrin fitting NS p _ to pump inlet Install filter on tubing Flangeless fitting amp ferrule 200 mL min Head Delrin fitting S m q Flangeless fitting amp ferrule h pump inlet Figure 7 Inlet filter assembly installation Installation of inlet tubing for 10 to 100 mL min heads 1 Remove the solvent inlet assembly from the accessories package and immerse the inlet filter into clean HPLC grade water 2 Assemble the inlet filter onto the end of the inlet tubing Purification Solution System User Guide Installation 3 3 Connect the inlet fitting to the inlet port on the pump head check valve the lower port Tighten the inlet tubing finger tight If leakage occurs in use
27. valve Ferrule Tubing SST SST Ferrule Tubing PEEK PEEK Semi Prep SST Semi Prep Titanium Compression fitting Titanium tubing Titanium ferrule over 172 bar Compression fitting tubing a Ferrule collar PEEK ferrule SS E over 172 bar 1 8 in tubing Compression fitting PEEK tubing l T Ferrule collar 1 PEEK ferrule p up to 172 bar CLT 1 Unit conversion 172 bar 2500 psi 17 2 MPa 2 Unit conversion 1 8 in 0 318 mm Purification Solution System User Guide 61 3 Installation Installing the Solvent Delivery Module Small opening at top Ss Figure 9 Detail of PEEK ferrule and ferrule collar 62 Purification Solution System User Guide Installation 3 Narrowbore and Analytical Stainless Steel Mixers Plumbing Fittings Homm w Extra long fitting and Nui Inlet fitting and Msi Figure 10 Compression fittings for 0 6 mL narrowbore and 1 2 mL analytical stainless steel mixers Tools required Description Wrench 1 4 in 1 For both the extra long fitting on the outlet and the two standard fittings for the inlet slide the fitting and ferrule over the 0 159 cm 1 16 in OD tubing and push the end of the tubing into the port as far as possible 2 Holding the tubing in place finger tighten the fitting then tighten 1 4 turn more with a wrench When tightening the outlet fitting hold the top of the piston steady with another wrench Purification Solution System User Guide
28. 4 7 e Using Troubleshooting Maintenance a an and Parts Using Troubleshooting Maintenance and Parts 136 This chapter provides information on how to access further details on the system components te Agilent Technologies 135 4 Using Troubleshooting Maintenance and Parts Using Troubleshooting Maintenance and Parts 136 For details on using troubleshooting maintenance and necessary parts for the individual modules please refer to the according manuals Agilent 218 Solvent Delivery Module User Manual G9300 90001 Agilent 325 UV VIS Dual Wavelength Detector User Manual G9309 90000 Agilent 410 Autosampler User Manual G9331 90000 Agilent 440 Fraction Collector User Manual G9340 90000 Purification Solution System User Guide This chapter provides information on cables used with the instrument Purification Solution System User Guide 5 Cables Cable Overview 138 Cable Connections 139 Analog Output 140 Relay Output 141 Desktop PC Communications 144 Synchronization Signals 145 ee Agilent Technologies 137 5 Cables Cable Overview Necessary cables p n Description 392612901 Ethernet cable for use in a network OR 5023 0203 Ethernet cable cross over for standalone use 392607969 Inject marker cable 392607975 Next injection cable 393546291 Serial communication ribbon 393597601 Converter RS 232 to RS 422 7910046300 Serial cable Optional cables p n Description 110743800
29. 422 393597601 2 Serial extension cable R000815107 Purification Solution System User Guide 107 3 Installation 2 Connect the 9 pin D shell connector on the RS 232 end of the Converter RS 232 to RS 422 893597601 to a serial communication port on the computer The screws on the RS 232 end of the RS 232 RS 422 Converter should be screwed into the nuts on the computer 9 pin D shell serial port connector 108 Purification Solution System User Guide Installation 3 Installing the Stream Splitter Agilent Stream Splitters are designed for preparative HPLC applications with flow rates up to 1200 mL min to split stream between preparative HPLC detector and an analytical detector If modules in the flow pass are not capable of the pressure the stream splitter must be installed PCG426000001 is designed for flow rates up to 200 mL min It uses 1 16 inch connection fittings PTFE tubing PCG426000002 is designed for higher flow rates up to 1200 mL min It uses 1 8 inch and 1 16 inch connection fittings PTFE tubing depending on the detector being used Some tubing s and fitting are supplied to connect with both preparative and analytical flow cells as required 1 Install the stream splitter as close as possible to the outlet of the UV detector the inlet of the ELSD and the fraction collector Cut the appropriate lengths of the chosen diameter tubing Install outlet from the UV detector to the IN port on the stream splitter
30. 63 3 64 Installation Analytical PEEK and Narrowbore Analytical Titanium Mixers Plumbing Fittings Extra long fitting Inlet fitting and ferrule Figure 11 Compression fittings for 1 2 mL analytical PEEK and 0 6 mL narrowbore and 1 2 mL analytical titanium mixers Do not use tools to tighten 1 Slide the 0 159 cm 1 16 in OD outlet tubing through the one piece fitting and ferrule and push the end of the tubing into the port as far as possible 2 Holding the tubing in place finger tighten the fitting For both inlet ports place a fitting and ferrule on the tubing and tighten in the same manner as the outlet fitting Purification Solution System User Guide Tools required Installation Preparative PEEK Titanium Mixers Plumbing Fittings Fitting Ferrule collar Ferrule Figure 12 Compression fittings for 10 mL preparative PEEK and titanium mixers Description Wrench 5 16 in All ports are identical 1 Slide the fitting and ferrule ferrule collar over the 0 318 mm 1 8 in OD tubing as shown and push the end of the tubing into the port as far as possible 2 Holding the tubing in place finger tighten the fitting When tightening the outlet port fitting hold the piston steady with another wrench 5 16 in When the system plumbing is complete check for any leaks If a leak is found stop the pumps and tighten the affected fitting just enough to stop the leak Purification Solution System User Guide 3
31. Agilent 218 Purification Solution System User Guide tt Agilent Technologies Notices Agilent Technologies Inc 2012 2013 No part of this manual may be reproduced in any form or by any means including elec tronic storage and retrieval or translation into a foreign language without prior agree ment and written consent from Agilent Technologies Inc as governed by United States and international copyright laws Manual Part Number 69300 90301 Edition 03 2013 Printed in Germany Agilent Technologies Hewlett Packard Strasse 8 76337 Waldbronn This product may be used as a com ponent of an in vitro diagnostic sys tem if the system is registered with the appropriate authorities and com plies with the relevant regulations Otherwise it is intended only for gen eral laboratory use Warranty The material contained in this docu ment is provided as is and is sub ject to being changed without notice in future editions Further to the max imum extent permitted by applicable law Agilent disclaims all warranties either express or implied with regard to this manual and any information contained herein including but not limited to the implied warranties of merchantability and fitness for a par ticular purpose Agilent shall not be liable for errors or for incidental or consequential damages in connection with the furnishing use or perfor mance of this document or of any information contained h
32. Autosampler G2260A Dual Loop Autosampler G2258A Fraction Collector G1364B Binary Pump G4220B Manual Injector G1328C combined with one or two Agilent 218 Pumps The option to combine several modules requires the Agilent OpenLAB CDS ChemStation software as master controller The 218 Solvent Delivery Module as master controller supports only the modules listed above Purification Solution System User Guide Introduction 1 Introduction to the Agilent 218 Solvent Delivery Module The Agilent 218 Solvent Delivery Module uses proven single piston rapid refill technology for economy reliability and virtually pulse free operation A range of interchangeable pump heads allows operation at flow rates from 10 uL min to 200 mL min Biocompatible pump heads are available for those analysts requiring a completely inert flow path A single channel analog to digital converter built in to each Agilent 218 Solvent Delivery Module can convert a detector signal to digital form and transmit the data to a computer system Five programmable analog inputs and three programmable relay outputs are available to further automate the HPLC system The Agilent 218 Solvent Delivery Module is easy to use and very flexible in operation It can be used in several different modes of operation as a standalone isocratic pump as either a master pump or a slave pump ina high pressure gradient system as a sample inject pump in a preparative system or in a fully a
33. EE 4 3 Verify the connection by clicking on Activate communication Information Communication established with COM1 is visible on the screen 4 Click OK Configuration dialog closes Purification Solution System User Guide 121 3 Installation Fraction Collector Configuration 1 Doubleclick in Configurable Modules on Fraction Detector Fraction Collector Configuration dialog opens 2 Select the COM Port as COM 2 r gt Fraction Collector Configuration Instrument 1 pense COM Port COM2 X Activate communication Serial number AY1101M015 Flow settings Volume delay 30 0s Rack and tube settings Rack type 60 12 zZ Probe depth 5mm Max tube time 1 00 min a Communication established with COM2 ok J cme J Hep __ 4 3 Fill in the Flow settings and the Rack and tube settings Verify the connection by clicking on Activate communication Verify the connection by clicking on Activate communication Information Communication established with COM2 is visible on the screen 122 Purification Solution System User Guide Installation 3 Setup the Software Terminating configuration 1 Before closing the Configure Instrument windows deselect the 3D spectral evaluation check box Selected Modes Agitent Prep LC Ato Configuration Qe verre ps rwrsxsono gt HH eao P5225 EL thi Posten cotector 4208 AYT 101S Generic
34. Head version One ore more SD 1 pumps have been configured in the instrument A 1 PrepStar SD 1 v Please make sure to select the stop action in the pressure screen of all a_j the pumps 2 8 2 PrepStar 0 1 Ten 1 Press the PRESS button on the pump 2 Select stop below the ACTION parameter with the arrow keys of the pump SD 1 CIM Control 7 Use SD 1 OM for injection CM ID 1 Activate communication ok Cancel Help The CIM is able to send a start or stop pulse 11 Click OK Configuration dialog closes 12 Click OK You re back in Agilent OpenLAB Control Panel 133 3 134 Installation in th PRO R R Edit Delete Refresh Lock Create Configure Edit Notifications Shortcuts Instrument Instruments and Locations Properties Actions Navigation Not Connected mm Instr i X PrepLC K Launch Offline Ey instruments X Administration Your startup license expires in 60 days 2 Set CIM parameters ee I E a ua BS Pepe gt Aosan Adcuonole rect Pogan P5325 E ouz Fraction colector E lt Instrument Curves Analog channel Acquisibon frequency dike bd Run tne 100m Relays Pulse wich 10s t LTO a T e e S ress Open Open Open lt mape Tene rar Rely 3 Rely 2 a Open 0pm I ii I E snow smestio graph L 3 Click OK to confirm method Purification Solution System User Guide R Purification Solution System User Guide e J a e ee
35. Modules emt crise ceriey wx Aak for configuration charge at Oam Raton aatup 2 Click OK You re back in Agilent OpenLAB Control Panel Purification Solution System User Guide 123 3 Installation Setup th Setup Method 1 Select Launch to start the OpenLab CDS Chemstation Edition PEO Ae R Edit Delete Refresh Edit Lock _ Create Configure Notifications Shortcuts Instrument Instruments and Locations Properties Actions Navigation Not Connected mam Instruments Start Instrument X Prepic a Launch Launch Offline EL instruments X Administration Your startup license expires in 60 days 124 Purification Solution System User Guide Installation 3 Setup the Software 2 Set Pumps parameters 3 Click OK to confirm method Purification Solution System User Guide 125 3 Installation Setup the Software 4 Set Autosampler parameters 5 Click OK to confirm method 126 Purification Solution System User Guide Installation 3 Setup the Software 6 Set Autosampler Injector Program 7 Click OK to confirm method Purification Solution System User Guide 127 3 Installation Setup the Software 8 Set PS 325 parameters W P5325 Fraton colector NOTE The Run time must be defined to the detector parameters 9 Click OK to confirm method 128 Purification Solution System User Guide Installation 3 Setu
36. NG High velocity stream of volatile and or toxic liquids If a line ruptures a relief device opens or a valve opens accidentally under pressure potentially hazardous high liquid pressures can be generated by the pump gt Wear personal protective equipment when you inject samples or perform routine maintenance gt Never open a solvent line or valve under pressure Stop the pump first and let the pressure drop to zero gt Always keep the doors and covers closed during operation gt Read and adhere to all Notes Cautions and Warnings in the manual Ultraviolet Radiation WARNING Irritation to the skin eyes and upper respiratory system gt Ensure that protective lamp covers of variable and fixed wavelength detectors are in place during operation gt Do not look directly into detector fluid cells or at the UV light source When inspecting the light source or fluid cell always use protective eye covering such as borosilicate glass or polystyrene gt Ventilate the area surrounding the detector such that the concentration of ozone does not exceed the maximum permissible level All venting must be to outside air never within the building Ozon generation Ozone can be generated by radiation from the source lamps The maximum permissible exposure level is 0 1 ppm 0 2 mg m Purification Solution System User Guide 155 6 Appendix Solvent Miscibility 156 Solvents should mix with each other in all pr
37. Setting the Pump ID and Pump Head Size When Using an Agilent 218 Pump as a Master Controller on page 70 Purification Solution System User Guide Installation 3 Pressure Module Installation Each Agilent 218 Pump can have a pressure module installed in the panel on the right side of the pump The pressure module dampens pump pulsations and supplies the current pressure value to the Agilent 218 software The pump needs this information to implement compressibility compensation and flow rate accuracy corrections and to ensure that system pressure is within the limits entered during setup Four pressure module ratings are available 60 0 MPa 600 bar 8700 psi 41 4 MPa 414 bar 6000 psi e 27 6 MPa 276 bar 4000 psi 13 8 MPa 138 bar 2000 psi 8 3 MPa 83 bar 1200 psi These have different flow rate max min see Agilent 218 Solvent Delivery Module User Manual G9300 90001 The pressure module is identified on the front of its panel The connector from the pressure module should be plugged into the master pump Only one pump in the HPLC system needs to have a pressure module installed Tools required Description Phillips head screwdriver You do not need to remove the top cover to install the pressure module or mixer The covers are removed in some photographs for clarity If you are using an HPLC system with a Agilent 218 as controller the pump with the pressure module should be the master controller device ID MC
38. T IT 2 lS Z lo 2 GEIS kx Figure 32 Solvent miscibility of some common solvents Purification Solution System User Guide Appendix 6 Solvent Compressibility The values in Table 12 on page 157 should be used for the Agilent 218 Purification Solution compressibility factors when you are setting up the pumping system parameters For details on how to set up the pump system parameters refer to Agilent 218 Solvent Delivery Module User Manual G9300 90001 Table 12 Compressibility factors Solvent x L Water 46 3231 Acetone 128 9 956 Acetonitrile 97 4 1212 Benzene 96 7 1046 Carbon tetrachloride 106 7 998 Chloroform 97 4 1227 Cyclohexane 114 800 Dichloroethane 111 9 1020 Diethyl ether 188 700 Dimethylformamide 80 1500 Dioxane 60 1500 Ethanol 115 1100 Ethyl acetate 100 1800 Methylene chloride 97 4 1212 Methanol 125 1200 n Heptane 144 760 n Hexane 167 2 644 o Dichlorobenzene 95 1400 Purification Solution System User Guide 157 6 158 Appendix Table 12 Compressibility factors Solvent x L Propanol 98 1200 Tetrahydrofuran 95 1500 Toluene 93 1200 2 Methylformamide 80 1500 Purification Solution System User Guide Appendix 6 The Waste Electrical and Electronic Equipment Directive Abstract The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC adopted by EU Commission on 13 February 2003 is introducing producer responsibility on all electric and electronic appliances
39. V usage gt Check that the voltage configuration is set correctly for your local power requirements gt If itis necessary to change the voltage configuration see maintenance procedure in Agilent 218 Solvent Delivery Module User Manual G9300 90001 1 Setup electrical connections 2 Check that the ON OFF power switch is off in the O position 3 Connect the power cord to the back panel of the module and plug it into a grounded power socket A good ground connection is necessary to ensure safety for users and proper communications 4 Turn on the power switch Purification Solution System User Guide 39 3 Installation If the pump does not start check following items 1 Proper connection of the power cord 2 Power at the wall receptacle 3 Functionality of the main power fuse F1 For fuse installation see maintenance procedure in Agilent 218 Solvent Delivery Module User Manual G9300 90001 The fuse rating and operating voltage is printed on the rear panel next to the power receptacle 40 Purification Solution System User Guide Installation 3 Installing the Solvent Delivery Module Pump Head Installation The pumps are shipped without the pump head installed You will have to install the pump head before beginning to run You can also change pump heads at any time Pump head Pump head clamp Thumbscrew or hexscrew Figure 5 Pump head installation Tools required Description Hex wrench 1 4 in
40. agent Addition Internal Standard addition sample dilution or derivatization can be programmed in a very simple manner A single stage derivatization of a sample in a separate destination vial requires no more than 4 program lines Multi reagent addition is also possible two large volume reagent vials are available on the sample tray Service Autosampler Low instrument down time is accomplished by a high Mean Time Between Failure and quick instrument service Special attention has been paid to these aspects of the concept as is illustrated by the injection valve The Agilent 410 Autosampler will alert you when the lifetime of the seal is exceeded or if the switching torque becomes too high This allows preventive maintenance before injection performance degrades And if necessary the entire injection valve can be replaced in seconds with the unique Quick fit valve mounting mechanism Purification Solution System User Guide 13 1 introduction Agilent 410 Preparative Autosampler G9332A By just choosing Prep mode in your system settings you can use the Agilent 410 Autosampler to inject all of your sample into a Preparative LC system or in other areas where large injection volumes are required The combination of large sample vials 10 mL a large sample volume needle and a 2 5 mL syringe enable you to inject large volumes very reproducible with high speeds and only 45 uL of sample loss The installed large bore valve 0 75 mm with
41. al injection valve and bracket installation 49 mast kit installation 47 mat rack location 98 method setup 124 0 outlet check valve connections 59 overview installation 96 module 85 P packaging damaged 28 parts 136 piston washing installation 57 plumbing fittings analytical stainless steel 63 analytical titanium mixers 64 narrowbore titanium 64 narrowbore 63 preparative PEEK 65 preparative titanium 65 plumbing safety 55 power cords 19 power 166 connection 104 prep option installing 94 pressure module installation 43 prime purge valve and bracket installation 51 pump head high pressure tubing installation 58 pump head installation 41 setting control software 69 pump ID setting control software 69 pump overview 9 rack location mat 98 rack orientation 103 radio interference 161 reagente addition 13 relay output 141 removing door 83 front panel 75 S safety information lithium batteries 160 safety general information 150 sample rack assembly 101 installation 102 sample tray installation 93 88 setup ChemStation control 116 electrical 39 hardware 110 OpenLab 116 pumpID 111 side port needle 12 site requirements power cords 19 specifications 21 spill tray installation 97 stack auto preparative 38 binary 37 isocratic 36 optimizing 35 synchronization signals 145 syringe waste tubing connecting 89 system rinsing 91 T te
42. al safety precautions must be observed during all phases of operation service and repair of this instrument Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design manufacture and intended use of the instrument Agilent Technologies assumes no liability for the customer s failure to comply with these requirements WARNING Ensure the proper usage of the equipment The protection provided by the equipment may be impaired gt The operator of this instrument is advised to use the equipment in a manner as specified in this manual 150 Purification Solution System User Guide Appendix 6 Information Symbols The following is a list of symbols that appear with warnings in this manual or on the liquid chromatograph The hazard they describe is also shown A triangular symbol indicates a warning The meanings of the symbols that may appear alongside warnings in the documentation or on the instrument itself are as follows Table 10 Warning symbols gt gt gt Broken glass Chemical Electrical hazard gt gt Explosion Eye hazard Fire hazard hazard Hot surface g ee lt f 9 9 lt lt weight danger to danger to hands gt gt Moving parts Respiratory Attention hazard 1 The symbol may be used on warning labels attached to the instrument When you see this symbol refer to the relevant operati
43. ampler Configuration QD suorana P5419 at 218 SD 1 Purp GE Puros PS21S01 ne B r 3 440 Fraction Colector Fraction Collector ae Low Flow Fraction Collector Sampler Q HP Sampler Additional configuration _careet_ __Heb 4 Click to add the modules to the Selected Modules The configuration dialog for the selected module opens 5 Doubleclick in Configurable Modules on 218 SD 1 CIM 218 SD 1 CIM Configuration dialog opens Select the COM Port the CIM Model Detector ID and enter the ID value 7 Verify the connection by clicking on Activate communication r 218 SD 1 CIM Configuration Instrument 1 a COM Port COM2 X 1D 5 CIM Model 210 218 X Detector ID 1 Ea Activate communication FW version CM300V1 1 i Communication established with COM2 OK ie Cancel Help E d 132 Purification Solution System User Guide Purification Solution System User Guide Installation 3 Information Communication established with COMn is visible on the screen 8 Click OK Configuration dialog closes 9 Doubleclick in Configurable Modules on 218 SD 1 Pump 218 SD 1 Pump Configuration dialog opens 10 Select the check box Pulse relay 1 of pump A on start 218 SD 1 Pump Configuration Instrument 1 fe COM Post cOM1 Z Number of pumps 2 Pressure un bar X Injection pump present EEA Pump action on pressure error mr Pump ID Model Senal number Fanware
44. ck Electrical power for the module must be provided through a three wire outlet with ground connection The outlet must be rated for at least 75 VA gt Ensure that power receptacles are earth grounded at the grounding pin 104 Purification Solution System User Guide Installation 3 Ensure that the probe arm s movement is not interfered with during operation initialization 1 Ensure that the power switch is turned off 0 2 Plug one end of the power cable into the module both the switch and the power socket are located on the connection panel on the pillar and the other into the mains power outlet In some countries it may be necessary to fit a suitable three pin power plug to the cord A three pin earthed power outlet must be used Ensure the module is always connected to the mains supply protective earth 3 Turn on the module The module will go through initialization tests and set the probe position If the initialization process is not successful refer to chapter Troubleshooting and Diagnostics in Agilent 440 Fraction Collector User Manual G9340 90000 If the fraction collector does not start up check each fuse as described in chapter maintenance in Agilent 440 Fraction Collector User Manual G9340 90000 During the initialization sequence 1 The probe rises to the full extreme of the Z axis 2 The probe travels to the full extremes of the X axis and rotates to the full extremes of the Theta axis 3 T
45. ct ID of the Pump 2 available choices are 0 63 MC master controller or no ID NOTE For OpenLAB an ID between 1 63 must be used HDSZ Pump head xx depends on what pump head is installed for choices see Note above Refill 125 piston CIM 4 Press Enter 5 Reboot the module 112 Purification Solution System User Guide Installation 3 Setup the Autosampler Preparation for general and tray settings 1 Press Serial on the autosampler keyboard To set up the general system settings 1 Turn the module on 2 Press SYSTEM 3 Press GENERAL 4 Confirm or change each of the settings that appear on the display Volume of installed loop 0 5000 uL Volume of tubing needlewvalve 0 999 uL Syringe volume 250 1000 Syringe speed LOW NORMAL HIGH Skip missing vials YES NO Air segment YES NO Headspace pressure YES NO To set up the tray settings 1 Turn the module on 2 Press SYSTEM 3 Press Tray 4 Confirm each of the tray settings that appear on the display Tray type 84 3 9 24 Vial type STANDARD 2 5 mL 5 Reboot the module Setup the Detector The detector is set up at the factory with a BOOTP or DHCP IP address If need be this can be changed by a trained service engineer who will own a copy of the diagnostics software or by setting up Agilent BootP Service to provide the detector with the right IP details Purification Solution System User Guide 113
46. cted to any voltage in the range 100 240 VAC 10 50 60 Hz 1 Hz single phase without modification or the need to change fuses Before connecting power to the detector ensure that the power switch on the front of the instrument is OFF the rocker switch O is pressed The power switch is a rocker switch that connects from the front of the detector directly to the power receptacle on the rear panel Plug one end of the power cord into the power receptacle on the rear panel and the other end into your AC power source Purification Solution System User Guide 73 3 Installation WARNING Absence of ground connection or use of unspecified power cord The absence of ground connection or the use of unspecified power cord can lead to electric shock or short circuit gt Never operate your instrumentation from a power outlet that has no ground connection gt Never use a power cord other than the Agilent Technologies power cord designed for your region 74 All devices in the liquid chromatography system should be connected to the same power source using a properly grounded 8rd wire to earth multiple outlet power strip Do not turn on the AC power yet All required external devices and hydraulics must first be connected Avoiding Harmful interferences to Radio or Television Reception Operation is subject to the following two conditions e This device may not cause harmful interference e This device must accept any
47. dispensed to the syringe waste 8 Repeat this action until the wash solvent tubing and the syringe are completely filled and no air bubbles appear in the syringe 9 Press Escape to leave the maintenance screen 10 After the wash solvent tubing and the syringe are filled press WASH to perform a standard wash routine Repeat the wash routine 2 or 3 times All tubing connected to the syringe valve will be rinsed with wash solvent The autosampler will give the best results if all air is removed from the syringe Purification Solution System User Guide 91 3 92 Connecting the HPLC to the Autosampler To ensure reproducible injections the following connections to your HPLC system should be made 1 Connect the HPLC pump to port 1 of the injection valve 2 Connect the HPLC column to port 6 of the injection valve 3 Check for leakage and let the system equilibate for at least 5 min The instrument is flushed with Ilsopropanol Make sure that the mobile phase of your HPLC system is miscible with lsopropanol If your mobile phase is not miscible with lsopropanol then start with an intermediate solvent that is a transition to the mobile phase To avoid possible contamination of the column it is good practice to not have the column installed during this operation It is essential that the contents of the sample loop are injected as a back flush onto the column therefore do not exchange column and pump connections at the injection valve
48. drain Purification Solution System User Guide CAUTION Installation 3 Connecting the Syringe Waste Tubing 1 Place the syringe waste tubing into a small bottle next to the autosampler If no injection volumes are programmed that are larger than the buffer tubing can contain the syringe waste will only be wash solvent Connecting the Water and Leakage Drain All solvents that result from a leak in the system and condensed water are drained through the left hand hose connector Risk of damaging the autosampler Drain and waste tubing are twisted thereby obstructing the flow path gt Be sure that the drain and waste tubing are not twisted 1 Connect the hose connector to a waste container on the floor Purification Solution System User Guide 89 3 Installation Starting the Autosampler Tools required OR Preparations Description Distilled Water lsopropanol Helium Ultrasonic bath Allow the Agilent 410 Autosampler to reach ambient temperature for at least one hour Connect the waste tubings see Connecting the Waste Tubings on page 88 for more information Electrical shock Risk of stroke and other personal injury due to reduced safety protection or unwanted fusing gt Ensure that the code on the fuse cap matchs the information next to the fuse holders Risk of fire and damaging the module gt For 115 VAC 15 use two 5 A T fuses slow blow gt For 230 VAC 15 use two 2 5 A T fuses
49. e 29 Pin designations for J10 and P9 P9 provides connections when the Agilent 325 UV VIS Dual Wavelength Detector acts as a slave and receives control from another module J10 provides connections when the detector acts as a master and sends control to another device An input is activated or said to be present when its two signal wires are connected together This can be done with a relay contact closure If the inputs are driven from another instrument with optical isolators or other polarized devices then attention must be paid to the polarity of the signal wire connections The positive output signal must be connected to the positive input signal and the negative output to the negative input Purification Solution System User Guide 145 5 Cables The color coding and physical design of the cable connectors ensure that correct signals and polarity are matched The outputs are optical isolators and simulate a relay contact closure when they are activated see Figure 30 on page 146 The minimum requirement for an input signal to be detected is 200 ms INPUT ey OUTPUT 57 Figure 30 Input output schematics 146 Purification Solution System User Guide Table 9 Signal Enable Out Ready In Ready Out Start In Start Out Fault In Fault Out Auto zero Lamp off Cables 5 Signal Description J10 and P9 Description A non polarized constantly active output a short This
50. e shown below Figure 27 Pin designation for J4 There are four general purpose output relays and one dedicated Peak relay Each output uses a DIP relay that is capable of handling 500 mA of contact current At reset or power up the output relay contacts are set to the default parameters open After loading a method they will be set as defined in the method s time 0 parameters Purification Solution System User Guide 141 5 Cables The Peak relay is software programmable for duration delay and active sense At power up the relay contact will be set to the inactive state as defined by the value of the active sense parameter stored in the detector Upon being triggered relay activation will occur for the time interval equal to the Peak Sense duration parameter as stored in the method The Peak relay can be activated from any of the following sources only one source can be active at any one time Time Slice event Once time slice has been turned on it will provide a periodic activation of the Peak Sense relay at an interval defined in Time Slice period within the method Time Slice can be turned on and off by time e Pulse event A single timed programmed activation of the Peak Sense relay as defined in the method Peak sense has been turned on To connect open ended wires to the relay signals available at J4 use the optional Assy PWB relay interface 325 210187590 see Figure 28 on page 142 Simply plug this PWB
51. e spill tray to ensure proper alignment see Figure 22 on page 102 1 Position the rack location mat over the spill tray and press the mat onto the locating tabs 2 Check that the rack location mat fits firmly in place by trying to move it side to side There should not be any movement If the rack location mat is loose refit it Installing the Probe and Tubing The sample probe comes preassembled with 0 050 cm 0 020 in ID tubing There are three additional coils of tubing included in the probe kit The longer piece of tubing with a nut and ferrule is used for the drain tube on the divert valve e The shorter tubing with a nut and ferrule is used to connect fraction collector divert valve to the sample output from the HPLC system e The third piece of tubing is 0 025 cm 0 010 in ID tubing and can be used for low delay volumes for applications with flow rates of 2 mL min or less For details on installing the tubing refer to Agilent 440 Fraction Collector User Manual G9340 90000 1 Turn off the fraction collector 2 Slide the Z axis slide to the top of the probe carriage 3 Manually rotate the probe arm so that it can be easily accessed 98 Purification Solution System User Guide Installation 3 4 Disassemble the probe kit by unscrewing the nut holding the metal probe from the bottom of the lower knurled mount nut and then the lower knurled mount nut from the top knurled mount nut Top knur
52. echnologies sales and service office immediately Inform your service representative that the instrument may have been damaged during shipment CAUTION Defective on arrival problems If there are signs of damage please do not attempt to install the module Inspection by Agilent is required to evaluate if the instrument is in good condition or damaged gt Notify your Agilent sales and service office about the damage gt An Agilent service representative will inspect the instrument at your site and initiate appropriate actions 28 Purification Solution System User Guide Installation 3 Check Delivery Delivery Checklists Delivery Checklist Pump The pump is packed in a single carton The pressure module ordered with the pump is shipped separately and needs to be installed Any pump head ordered with the pump is packed separately The following list shows all items delivered in a standard delivery Your personal list depends on your order therefore countercheck delivery with your order Agilent 218 Solvent Delivery Module e Power cord Pump head kit Plumbing kit G9300A e Pressure transducer module G9300A Mast kit G9301A Internal mixer G9301A Delivery Checklist Detector The following list shows all items delivered in a standard delivery Your personal list depends on your order therefore countercheck delivery with your order e Agilent 325 UV VIS Dual Wavelength detector Assy PWB Sync Interface 325 335
53. ed in Prep Upgrade Option Includes Needle Syringe Injection Valve and Large Volume Sample Tray 393590791 Re connect all tubing to the injection valve Replace standard Syringe with the 2500 uL syringe Install the 24 vials LSV tray Choose Prep Mode in system Purification Solution System User Guide Installation 3 Installing the Fraction Collector Installing the Fraction Collector Overview of the Module i a 2 10 mo Eo i Eert l Back 3 11 SH 7 all 9 y Figure 20 Fraction collector components 1 Theta axis 2 Z axis slide 3 Top knurled mount nut on the probe assembly 4 Probe mounting block 5 Lower knurled mount nut on the probe assembly Purification Solution System User Guide 95 3 Installation 6 Nut holding the metal probe 7 Probe 8 Rack 9 Rack location mat 10 Split retaining ring 11 Fraction collector pillar control column Installation Overview Basic installation involves e Assembling the fraction collector Connecting the fraction collector to the HPLC system Installing Agilent OpenLAB CDS ChemStation PrepLC Drivers e Determining the delay volume and probe depth All external DIP switches should always be in the OFF position Assembling the Fraction Collector Carefully follow the instructions provided below to assemble your instrument Assembling the Agilent 440 Fraction Collector includes installing the 1 Spill tray 2 Rack locatio
54. el Tubing Kit 1 16 x 0 02 i d Isocratic Pump Stainless Steel Tubing Kit 1 16 x 0 03 i d Isocratic Pump Stainless Steel Tubing Kit 1 16 x 0 04 i d Isocratic Pump Stainless Steel Tubing Kit 1 8 x 0 08 i d In addition a Technical Note with installation instructions is included Delivery Checklist User Documentation CD Part of a standard delivery is also the Agilent Purification amp Preparative LC User Documentation CD G9300 64500 Purification Solution System User Guide 31 3 Installation Unpacking and Inspection Unpacking and Inspection 1 Check carefully to make sure you received all the items listed on the packing list 2 Carefully unpack all the containers and inspect the contents for damage as soon as possible 3 Save the packing containers they will be useful if you have to file a claim for damage or in the case of future transit Unpacking the Solvent Delivery System WARNING Danger to hands and feet The instrument is heavy gt Always use a fork lift or other suitable lifting device when moving the instrument CAUTION Overheating of the pump Objects interfering with airflow to the pump gt Maintain at least 15 cm 6 inches clear space next to the fan 32 Purification Solution System User Guide Installation 3 Unpacking the Detector Prerequisites Ensure there is enough room on the bench for the detector Heavy weight The Agilent 325 UV VIS Dual Wavelength Detector weighs i
55. em is connected with the serial interface cable To distinguish one module from another each module has a unique ID number This number must be entered in each Agilent 218 Pump using its own keypad The ID number of the master pump is set by the user to MC Slave pump ID numbers can be set as desired Each system can have only one master pump Slave pump IDs can also be set from a master pump from the BUS IDs menu Purification Solution System User Guide Installation 3 Installing the Solvent Delivery Module External Contacts Connectors J3 J2 INJECT Figure 13 External contacts connector The Agilent 218 Pump has a number of analog and digital connections on the rear panel These can be used to digitize data from an analog detector start and stop other devices through contact closures and to receive contact closures to start and stop the pump Connections are made to this strip by two connectors included in Standard accessory kit 893550991 These two connectors are then attached to another device Purification Solution System User Guide 67 3 68 Installation Table5 J3 terminal strip Name Connection INTGR HI Positive wire from detector INTGR LO Negative wire from detector INTGR GND Ground wire from detector CHASSIS GND Ground wire to chassis ANALOG OUT 0 10 V output signal Specifies which option to output as an analog ANALOG OUT GND 1 2 3 signal to a recording device Programmable opt
56. erein Should Agilent and the user have a separate written agreement with warranty terms covering the material in this document that conflict with these terms the warranty terms in the sep arate agreement shall control Technology Licenses The hardware and or software described in this document are furnished under a license and may be used or copied only in accor dance with the terms of such license Restricted Rights Legend If software is for use in the performance of a U S Government prime contract or subcon tract Software is delivered and licensed as Commercial computer software as defined in DFAR 252 227 7014 June 1995 or as a commercial item as defined in FAR 2 101 a or as Restricted computer soft ware as defined in FAR 52 227 19 June 1987 or any equivalent agency regulation or contract clause Use duplication or dis closure of Software is subject to Agilent Technologies standard commercial license terms and non DOD Departments and Agencies of the U S Government will receive no greater than Restricted Rights as defined in FAR 52 227 19 c 1 2 June 1987 U S Government users will receive no greater than Limited Rights as defined in FAR 52 227 14 June 1987 or DFAR 252 227 7015 b 2 November 1995 as applicable in any technical data Safety Notices CAUTION A CAUTION notice denotes a hazard It calls attention to an operating procedure practice or the like that if not co
57. gth Detector is not shipped with a flowcell installed You will need to install the flowcell that you purchased with the detector Each flowcell comes with a set of recommended nuts and ferrules that may be fitted to tubing 1 16 tubing is used on all flowcells However for the 4 mm x 0 15 mm super prep flowcell it is recommended to use 1 8 tubing at higher flow rates In this case you can add the Adaptor 1 8 1 16 1610126800 This will require the 1 8 tubing and the 1 8 flowcell fittings Tubing connections are PEEK type except for the Super Prep flowcell which uses ETFE tubing 0 125 mm x 0 062 mm Parts required p n Description 1 210181800 Flowcell 9 mm x 0 mm inert analytical OR 1 210181900 Flowcell 9 mm x 1 mm inert prep OR 1 210224200 Flowcell 4 mm x 0 25 mm inert scale up OR 1 210182100 Flowcell 4 mm x 0 mm inert micro analytical 1 9910128300 Flow cell replacement fittings 1 210182000 Flowcell 4 mm x 0 15 mm inert super prep 1 1610126900 Fitting 1 8 tube nut flat bottom 1 1610126400 Fitting 1 8 tube ferrule Pack of 10 1 1610126800 Adaptor 1 8 1 16 Purification Solution System User Guide 77 3 Installation Installing the Detector Ferrule Nut PEEK tube 1 Fit the nuts and ferrules on the PEEK tubes 2 Screw the two tubes into the inlet and outlet connection of the flowcell Each flowcell has an inlet and outlet connection NOTE Be careful not to cross thread the connecto
58. hapter provides information on cables used with the instrument 6 Appendix This chapter provides addition information on safety legal and web Purification Solution System User Guide 3 Contents Contents 1 Introduction 7 Introduction to the System 8 Introduction to the Agilent 218 Solvent Delivery Module 9 Introduction to the Agilent 325 UV VIS Dual Wavelength Detector 11 Introduction to the Agilent 410 Autosampler 12 Introduction to the Agilent 440 Fraction Collector 15 2 Site Requirements and Specifications 17 Site Requirements 18 Specifications 21 3 Installation 23 Delivery 26 Damaged Packaging 28 Check Delivery 29 Unpacking and Inspection 32 Optimizing the Stack Configuration 35 Installing the Solvent Delivery Module 39 Installing the Detector 71 Installing the Autosampler 84 Installing the Fraction Collector 95 Installing the RS 422 485 Communication Kit 107 Installing the Stream Splitter 109 Setup Hardware 110 Setup the Software 115 4 Using Troubleshooting Maintenance and Parts 135 Using Troubleshooting Maintenance and Parts 136 4 Purification Solution System User Guide Contents 5 Cables 137 Cable Overview 138 Cable Connections 139 Analog Output 140 Relay Output 141 Desktop PC Communications 144 Synchronization Signals 145 6 Appendix 149 General Safety Information 150 Solvent Miscibility 156 Solvent Compressibility 157 The Waste Electrical and Electronic Equipment Direct
59. he connection leaks when the system is pressurized tighten the nut slightly until the leak stops Connections for the outlet check valve 200 mL min Head The 200 mL min head uses 0 318 cm 1 8 in tubing and fittings on its outlet The pressure module used with the 200 mL min head also uses 0 318 cm 1 8 in tubing and fittings 100 mL min Head The pressure module comes with two 0 318 cm 1 8 in to 0 159 cm 1 16 in adapters for use with the 100 mL min head The outlet of the 100 mL min head is 0 159 cm 1 16 in tubing and fittings Refer to Figure 9 on page 62 for the appropriate outlet connections for your pump The appropriate compression fitting is in the accessories package Typically they are 4 28 male nuts and ferrules Purification Solution System User Guide 59 3 Installation Table 4 Connections for the outlet check valve Outlet valve Ferrule Tubing SST SST Ferrule Tubing Titanium Titanium Ferrule Tubing PEEK PEEK Analytical SST Compression fitting a SST tubing SST ferrule Analytical Titanium at a Compression fitting Compression fitting cae i PEEK tubing Titani bi a see Ferrule collar r itanium ferrule PEEK ferrule over 172 bar up to 172 bar 60 Purification Solution System User Guide Table4 Connections for the outlet check valve Installation 3 Ferrule Tubing Titanium Titanium Outlet
60. hen the probe is positioned at the front left of the fraction collector Instrument Communication Port The instrument communication port see Figure 24 on page 104 is used to connect the Agilent 440 Fraction Collector G9340A to the computer or MIB controlling the instrument Auxiliary Communication Port The auxiliary communications port provided on the Agilent 440 Fraction Collector G9340A is not used with current Agilent software or hardware Purification Solution System User Guide 105 3 Installation Connecting the Fraction Collector to the HPLC System For details on setting up the fraction collector to the HPLC System see Setup the System with OpenLAB CDS ChemStation Edition Control Panel on page 116 Determining the Delay Time 1 For information refer to the online help of the Agilent 440 Fraction Collector Determining the Correct Probe Depth 1 For information refer to the online help of the Agilent 440 Fraction Collector 106 Purification Solution System User Guide Installation 3 Installing the RS 422 485 Communication Kit Installing the RS 422 485 Communication Kit 1 Connect the Serial extension cable R000815107 to the end of RS 422 connector on the Converter RS 232 to RS 422 893597601 Connection to the serial port on the computer Connection of the RS 422 Communication Cable to the RS 422 end of the RS 232 RS 422 Converter To Purification System 1 Converter RS 232 to RS
61. hould be removed with the connecting tubing fitted These must be removed outside the detector compartment 7 Perform a lamp calibration see chapter maintenance in Agilent 325 UV VIS Dual Wavelength Detector User Manual G9309 90000 80 Purification Solution System User Guide Installation 3 Detector Outlet Back Pressure Restrictor The Back pressure restrictor 110743300 should be added to all flow cells unless a fraction collector is installed after the detector The back pressure restrictor assembly that was supplied with your detector should be threaded into the outlet line from the flowcell The back pressure restrictor applies approximately 2 76 bar 40 psi back pressure on the flowcell This prevents outgassing and bubbles from forming or being trapped in the flowcell which can cause an unstable baseline Note the arrow stamped on the restrictor body This arrow must point away from the flowcell outlet port and toward the waste receiver The threaded plastic fittings should be finger tightened only enough to prevent leaks About 122 cm 48 of 1 6 mm 1 16 tubing is supplied at both the inlet and outlet of the restrictor The inlet tubing is provided with a 1 6 mm 1 16 plastic tubing fitting for connecting to the flowcell outlet port Either the outlet tubing can be directed to the waste container or the tubing removed and the restrictor itself dropped to the bottom of the waste bottle The restrictor press
62. indow to set Pump ID Head size compressibility factors and refill speed For more information about setting the pump ID and pump head size see Setup in Agilent 218 Solvent Delivery Module User Manual G9300 90001 Purification Solution System User Guide Installation 3 Installing the Detector Location of the Detector Module Place the detector conveniently near your HPLC system The modular design of the detector enables you to locate it anywhere within the limitations imposed by the length of the power cord fluid lines and signal cables In order to keep liquid dead volume as low as possible and to minimize peak broadening in the lines the distance between the column outlet and the flowcell inlet should be kept to a minimum For best performance the detector should be located on a clean sturdy vibration free bench in an area free of Heat sources such as direct sunlight or a heater vent Drafts such as an open doorway window or air conditioner vent e Smoke or UV absorbing vapor Corrosive or dusty atmosphere Potential liquid spills Provide approximately 4 inches of space behind the unit so that the cooling fan intake is not impeded and to allow easy access to the rear panel services see Power Connection and Rear Panel Services on page 72 Purification Solution System User Guide 71 3 Installation Power Connection and Rear Panel Services WARNING Electrical shock Risk of stroke and other personal
63. injury gt Turn off the power to the detector before making power and signal cable connections The detector can communicate with other modules and devices in the entire HPLC system All power and signal connections are made on the rear panel of the detector see Figure 14 on page 72 The connectors on the rear panel support communication configurations to a wide range of Agilent modules and non Agilent devices ce C amp ely 5 SA TE qm Main power receptacle J1 Comm J4 Relay out J14 Analog out P9 Sync signal J10 Sync signal Figure 14 Agilent 325 Detector rear panel services The following table summarizes the function of each connection 72 Purification Solution System User Guide Installation 3 Table 7 Rear panel services functions Connection Function Main power receptacle 3 pin receptacle with 2 fuses J14 Analog out 9 pin female D shell connector used for two channels A and B of analog output J4 Relay out 15 pin female D shell connector used for time programmed contact closures J1 Comm RJ 45 type connector used to interface the Agilent 325 to a desktop PC P9 Sync signal 15 pin male D shell connector used with synchronization signal cable J10 Sync signal 9 pin female D shell connector used with synchronization signal cable For more information about the connections see chapter Cables AC Power The Agilent 325 UV VIS Dual Wavelength Detector may be conne
64. insing the System with Wash Solvent 91 Connecting the HPLC to the Autosampler 92 Filling and Sealing the Vials 92 Loading the Sample Tray 93 Installing the Agilent 410 Autosampler Prep Option 94 Installing the Fraction Collector 95 Overview of the Module 95 Installation Overview 96 Assembling the Fraction Collector 96 Installing the Spill Tray 97 Installing the Rack Location Mat 98 Installing the Probe and Tubing 98 Assembling the Sample Racks 101 Installing the Sample Racks 102 Rack Orientation 103 Connecting the Power to the Fraction Collector 104 Connecting the Fraction Collector to the HPLC System 106 Determining the Delay Time 106 Determining the Correct Probe Depth 106 Purification Solution System User Guide Installation 3 Installing the RS 422 485 Communication Kit 107 Installing the Stream Splitter 109 Setup Hardware 110 Setup the Hardware 110 Setup the Pump 111 Setup the Autosampler 113 Setup the Detector 113 Setup the Fraction Collector 114 Setup the Software 115 Install Agilent OpenLAB CDS ChemStation PrepLC Drivers 115 Setup the System with OpenLAB CDS ChemStation Edition Control Panel 116 Setup Method 124 Setup the CIM in the OpenLAB CDS ChemStation PrepLC Drivers 130 This chapter gives information about the installation of your Agilent 218 Solvent Delivery Module Agilent 325 UV VIS Dual WL Detector Agilent 410 Autosampler and Agilent 440 Fraction Collector Purification Solutio
65. internal fault condition itself This edge triggered contact closure causes an auto zero adjustment This edge triggered contact closure switches the lamp off It is possible to turn the lamp back on manually if the contact is still closed An optional Assy PWB sync interface 325 210186590 is available to interface between the Agilent 325 UV VIS Dual Wavelength Detector synchronization signals and other devices This board is inserted into the P9 and J10 connectors and connects to a terminal strip on the adapter board This terminal strip accepts bare wire leads from cables connecting other devices These cables may originate from the other device or a dedicated cable can be used if available for the particular application Purification Solution System User Guide 147 5 Cables Synchronization Signals 3 G C Q E e v Figure 31 The 0 adapter board 148 Purification Solution System User Guide Purification Solution System User Guide 6 Appendix General Safety Information 150 Solvent Miscibility 156 Solvent Compressibility 157 The Waste Electrical and Electronic Equipment Directive 159 Batteries Information 160 Radio Interference 161 Electromagnetic Compatibility 162 Agilent Technologies on Internet 164 This chapter provides addition information on safety legal and web Apg Agilent Technologies 149 6 Appendix General Safety Information General Safety Information The following gener
66. ions are A B C D solvent composition pressure system pressure nm wavelength specified in the 1 0 window flow system flow rate or off Full scale voltage is 10 V Ground wire for Analog Out Contact closure relay outputs These can be used to start external devices such as an autosampler 1 Do not connect to Chassis Gnd Table6 J2 terminal strip Name Connection AUX 5V 5 V positive signal AUX GND Ground for auxiliary voltage STOP Contact closure input to stop the pump from an external device D GND Digital ground for Stop signal HOLD Contact closure input from an external device to Hold a running method at the current time and flow composition conditions D GND Digital ground for Hold signal TRANSFER Contact closure input to Transfer to another method from an external device Transfer can be immediate deferred until the end of the current method pass or automatic at the end of run if no contact closure is received D GND Digital ground for Transfer signal Purification Solution System User Guide Installation 3 Table6 J2 terminal strip Name Connection INJECT Contact closure input from an external device which cancels a programmed Inject Wait or Hold D GND Digital ground for Inject signal MARK Contact closure input to perform an Event Mark a 10 vertical trace on the chromatogram from an external device D GND Digital ground for Mark signal
67. ite Requirements and Specifications 2 Power Cords Different power cords are offered as options with the module The female end of all power cords is identical It plugs into the power input socket at the rear The male end of each power cord is different and designed to match the wall socket of a particular country or region Absence of ground connection or use of unspecified power cord The absence of ground connection or the use of unspecified power cord can lead to electric shock or short circuit gt Never operate your instrumentation from a power outlet that has no ground connection gt Never use a power cord other than the Agilent Technologies power cord designed for your region Use of unsupplied cables Using cables not supplied by Agilent Technologies can lead to damage of the electronic components or personal injury gt Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Unintended use of supplied power cords Using power cords for unintended purposes can lead to personal injury or damage of electronic equipment gt Never use the power cords that Agilent Technologies supplies with this instrument for any other equipment Purification Solution System User Guide 19 2 Site Requirements and Specifications Condensation CAUTION Condensation within the module Condensation will damage the system electronics
68. ive 159 Batteries Information 160 Radio Interference 161 Electromagnetic Compatibility 162 Agilent Technologies on Internet 164 Purification Solution System User Guide Contents Purification Solution System User Guide 1 Introduction Introduction to the System 8 Introduction to the Agilent 218 Solvent Delivery Module 9 Introduction to the Agilent 325 UV VIS Dual Wavelength Detector 11 Introduction to the Agilent 410 Autosampler 12 Agilent 410 Autosampler G9331A 12 Agilent 410 Preparative Autosampler G9332A 14 Introduction to the Agilent 440 Fraction Collector 15 This chapter introduces to the Agilent 218 Purification Solution system and its components Apg Agilent Technologies 7 1 introduction Introduction to the System A complete Agilent 218 Purification Solution system includes Agilent 218 Solvent Delivery Module s Tubing Mast kit Agilent 325 UV VIS Dual WL Detector Agilent 410 Autosampler Agilent 218 Solvent Delivery Module Agilent SD 1 Isocratic Solvent Delivery Module for sample injection and Agilent 440 Fraction Collector As an option the HPLC Control Software Agilent OpenLAB CDS ChemStation Edition is recommended The Agilent 218 Purification Solution allows to combine the modules listed above with the following Agilent 1200 Infinity Series modules Diode Array Detector G1315 C D Multiple Wavelength Detector G1365 C D Variable Wavelength Detector G1314 B C E F Preparative
69. led mount on the probe assembly I Lower knurled mount nut on the probe assembly Insert the probe into the probe mounting block see Figure 20 on page 95 co 6 Push the tubing down into the probe so that at least 0 5 cm tubing protrudes from the end of the probe Secure the lower knurled mount nut to the top knurled mount nut co Secure the nut holding the metal probe into the bottom of the lower knurled mount nut 9 Clip the tubing into the tube restraint which is the small hook at the top of the Z axis slider Allow for a small length in the tubing to prevent kinking the tubing 10 Run the tubing along the back side of the probe arm housing Purification Solution System User Guide 99 3 Installation Installing the Fraction Collector 11 Attach the split ring also called the retaining ring as shown below The retaining ring is split to attach it through the loop on the underside of the probe arm housing a Split ring retaining ring 12 Feed the tubing through the retaining loop attached to the underside of the housing 13 Screw the tubing fitting into the bottom port 3 of the valve Port 1 to waste Port 2 from detector outlet or back pressure restrictor Port 3 to the probe NOTE It is important to ensure that the length of the tubing from the probe to the valve allows the probe arm to move freely in all axes If it is too tight it will restrict movement and
70. may cause movement failure If it is too loose the probe arm may become tangled in the tubing as it moves Tubing length between the valve and the detector depends on fraction collector placement When choosing the length of tubing ensure it is long enough to allow free movement of the arm but not too long to delay sample delivery into the tubes If the tubing is too long between the detector output and the fraction collector probe end your samples may not correspond correctly to the fraction collector markers on your chromatogram The delay volume calculation will compensate for this effect 14 Move the probe arm to the front right side of the fraction collector and rotate the probe arm fully to the right to check that the tubing is completely free to move 15 Manually position the probe arm in the middle along the X axis 16 Rotate the probe arm through its full extent to check that the tubing is free to allow full movement in all directions 100 Purification Solution System User Guide Installation 3 If the length is not correct remove and then replace the tubing install the fittings and tubing onto the fraction collector Repeat step 14 on page 100 step 16 on page 100 to test the tubing length 17 Adjust the length of the sample line from the HPLC system In port 2 see Figure on page 100 screw in the fitting with ETFE tubing Connect the other end of this tubing to the HPLC system s detector outlet or to the back pressure restrict
71. n System User Guide 25 3 Installation Delivery For detailed information on parts delivered with the modules refer to the corresponding module manuals e Agilent 218 Solvent Delivery Module User Manual G9300 90001 e Agilent 325 UV VIS Dual Wavelength Detector User Manual G9309 90000 Agilent 410 Autosampler User Manual G9331 90000 e Agilent 440 Fraction Collector User Manual G9340 90000 e And to the User Manuals of the Agilent 1200 Infinity Series which allows combining with the modules listed above see Introduction to the System on page 8 Components of a Complete System A complete system comprises following modules as ordered Hardware Modules p n Description G9300A Agilent 218 lsocratic Solvent Delivery Module G9301A Agilent 218 Add On Solvent Delivery Module OPTIONAL G9309A Agilent 325 UV VIS Dual WL Detector G9331A Agilent 410 Autosampler G9332A Agilent 410 Preparative Autosampler OPTIONAL G9340A Agilent 440 Fraction Collector 26 Purification Solution System User Guide Software p n M8366 64000 M8367 64000 M8500AA M8301AA Purification Solution System User Guide Installation Description OpenLAB CDS Installation Driver Prep LC OpenLAB CDS Installation Upgrade Driver Prep LC LC driver OpenLAB CDS ChemStation Edition Workstation A 01 05 or higher 27 3 Installation Damaged Packaging If the delivery packaging shows signs of external damage please call your Agilent T
72. n excess of 15 kg 33 Ib gt Carry the instrument at least with 2 people gt Avoid back strain or injury by following all precautions for lifting heavy objects gt Ensure that the load is as close to your body as possible gt Ensure that you can cope with the weight of your load 1 Carefully unpack the unit from the shipping carton and place it on the bench 2 Make sure to check carefully for all miscellaneous components that might be contained in the inner compartments The detector is a sensitive instrument and should always be handled with the degree of care appropriate for laboratory instrumentation Keep the shipping carton as it provides excellent protection if you have to transport or store the detector in the future Purification Solution System User Guide 33 3 Installation Unpacking the Autosampler CAUTION Risk of damaging the autosampler gt Do not lift the Agilent 410 Autosampler by the front cover 1 Lift the Agilent 410 Autosampler as shown in Figure 1 on page 34 with both hands under the instrument or with one hand under the front and the other hand grasping the rear top of the Agilent 410 Autosampler Figure 1 Agilent 410 Autosampler lifting instructions 34 Purification Solution System User Guide Installation 3 Optimizing the Stack Configuration This section contains information on how to stack your modules depending on the preparative method For optimal performance in other c
73. n mat 3 Probe and tubing 4 Sample racks Position the fraction collector on the side of the HPLC system closest to the detector output to help decrease the delay volume 96 Purification Solution System User Guide Installation 3 Installing the Fraction Collector Installing the Spill Tray Alignement pin underneath the raised location tabs Back side of the fraction collector closest to the fraction Figure 21 Spill tray alignment pin and mat for the rack location raised locating tabs Preparations Position the fraction collector closest to the detector output This decreases the delay volume NOTE The spill tray will catch most solvent spills Always clean up spills immediately 1 Locate the alignment pins protruding above the base support tube 2 Lower the tray so that the two alignment pins closest to the fraction collector pillar fit into the slots in the spill tray 3 Lower the spill tray until the front edge rests on the front of the base support tube 4 Check that the spill tray is firmly pushed down on all alignment pins NOTE The spill tray must be firmly in position so that it does not move Purification Solution System User Guide 97 3 Installation Installing the Rack Location Mat The rack location mat is installed on top of the spill tray Preparations Spill tray installed There are cut outs around the rack location mat The cut outs slot over the raised locating tabs that are molded into th
74. nd manually changed during an analysis as each rack s analysis is completed The rack closest to the rear of the fraction collector next to the pillar is considered rack number one Purification Solution System User Guide 15 1 introduction Introduction to the Agilent 440 Fraction Collector 16 Purification Solution System User Guide R Purification Solution System User Guide e a e r a e e ee 2 7 e Site Requirements and Specifications amp o Site Requirements 18 Specifications 21 This chapter provides infromation on site requirements and specifications of your system ea Agilent Technologies 2 Site Requirements and Specifications Site Requirements Power Considerations The instrument power supply has wide ranging capability It accepts any line voltage in the range described in Physical Specifications Hazard of electrical shock or damage of your instrumentation can result if the devices are connected to a line voltage higher than specified gt Connect your instrument to the specified line voltage only CAUTION Inaccessible power plug In case of emergency it must be possible to disconnect the instrument from the power line at any time gt Make sure the power connector of the instrument can be easily reached and unplugged gt Provide sufficient space behind the power socket of the instrument to unplug the cable 18 Purification Solution System User Guide S
75. nge peaks up to 40 AU cm with appropriate flowcell Purification Solution System User Guide 11 1 introduction Introduction to the Agilent 410 Autosampler The Autosampler is available in two configurations as the Standard Autosampler G9331A and the Preparative Autosampler G9332A Agilent 410 Autosampler G9331A The Agilent 410 Autosampler has been designed to meet the needs of the modern analytical laboratory The autosampler has the following features e Column temperature control and sample cooling guaranting consistent results e High resolution syringe control guaranting superior precision for injection and reagent addition Fast replacement of the injection valve Loop injection with Pressure Assisted Sample Aspiration is a proven concept that combines high precision with simplicity and reliability Three injection modes can be selected Full loop e Partial loop filling uL Pick up Maximum precision maximum flexibility and zero sample loss can be achieved with these features Side Port Needle The strong side port needle combines the optimum point style for septa piercing with a minimum risk of blockage by septum particles 12 Purification Solution System User Guide Introduction 1 Column Oven A column oven is an integral part of the Agilent 410 Autosampler because constant column temperature is important for long term stability of a chromatographic separation and may be required for GLP compliance Re
76. npack the stainless steel purge valve and purge valve bracket t ca 2 Remove the knob and nut from the purge valve The knob is held in place with a grub screw which is loosened using a 2 mm hex key Grub screw Nut 0o a 3 Secure the valve to the valve bracket using the nut 4 Replace the knob on the valve and tighten the grub screw using the provided 2 mm hex key 5 Attach the purge valve to the mast For correct height see Isocratic System on page 36 or Binary System on page 37 Purification Solution System User Guide 51 3 Installation Installing the 3 way Pump Head Prime Valve Tools required Description Hex key 2 mm Parts required Description Pump head prime valve Pump head prime valve bracket 1 Unpack the 3 way valve and bracket 3 Unscrew the nut and then remove the two washers from the 3 way valve 52 2 Using the provided 2 mm hex key loosen the grub screw and slide off the selection knob Valve body Flat washer Ridget washer Selection knob Grub screw Nut 4 Attach the bracket so that the three holes on the 3 way valve face away from the thumbscrew on the bracket Flat side of metal il post Holes in 3 way valve body Purification Solution System User Guide Installation 3 6 Slide the selection knob onto the 3 way valve The CAUTION engraved lines on the top of the selection knob should Damage t
77. o the fittings point to the holes in the black body of the 3 way valve The grub screw should be flush with the flat side of the metal post on the 3 way valve gt Finger tighten the nut and use the provided hex key to tighten the selection knob 5 Place the flat washer and then ridged washer on the 3 way valve Screw on the nut to secure the two washers Next Steps 7 Secure the selection knob to the 3 way valve using only the provided 2 mm hex key Do not over tighten 8 Attach the 3 way valve to the mast See Table 2 on page 36 for the correct height If you have a binary system repeat this procedure for the second 3 way pump head prime valve For correct height see Table 3 on page 37 Purification Solution System User Guide 53 3 Installation Installing the Column Hanger Parts required p n Description 1 R000048610 Analytical column hanger for 1 4 in OD OR 1 R000048602 Preparative column hanger for 1 in OD OR 1 R000048601 Preparative column hanger for in OD 1 Attach the column hanger on to the mast so that the column inlet is close to the injection valve Avoid blocking the other components 2 Secure the column hanger by tightening the black thumbscrew on the bracket 54 Purification Solution System User Guide Installation 3 Plumbing Connections CAUTION Damage to the pump heads gt Each individual pump head has a different set of tubing that comes with the pump head for connection to
78. on or service manual for the correct procedure re ferred to by that warning label Purification Solution System User Guide 151 6 Appendix Table 11 Information symbols Symbol Description l Mains power on 0 Mains power off eS LA Single phase alternating current Direct current Confirms that the product complies with the requirements of all applicable European Community directives 152 Purification Solution System User Guide Appendix 6 Solvent Hazards WARNING Explosion fire asphyxiation This instrument is not explosion proof Certain solvents may cause weakening and leaks of tubings or fitthings with possible bursting Even small leaks in solvent supply systems can be dangerous gt Only use solvents compatible with the HPLC system tubings and fittings gt Employ static measuring and static discharge devices to safeguard against the buildup of static electricity gt In unattended operation do not use organic solvents having an ignition point below 70 C gt Do not bring a heat or flame source near the instrument gt The area in which solvents are stored and the area surrounding the instrument must be adequately ventilated to prevent accumulations of gas gt Always check the condition of the instrument leakage of solvent or waste solution leakage of solvent inside the instrument If an abnormality is found stop operation immediately gt When using flammable chemicals be
79. onfigurations than in the given examples respect the following rules e The orientation is also suggested if you are only installing the Agilent 218 Pump If an autosampler is present it should be placed on top of the detector e Position the master pump as highest pump in the stack The pump with the pressure module is the master pump In gradient systems the remaining pumps are slave pumps This position allows easy access to keypad and display to control the HPLC system via pump instead of software Purification Solution System User Guide 35 3 Installation Optimizing the Stack Configuration Isocratic System Detector Injection valve Prime purge valve Low pressure solvent valve Pressure module Figure 2 Isocratic System Table 2 Isocratic system component heights on the mast measured from the bench to the bottom of the bracket Part Height in mm in Injection valve 285 11 2 Prime purge valve 185 7 3 Low pressure solvent valve 100 3 9 36 Purification Solution System User Guide Installation 3 Optimizing the Stack Configuration Binary System Pressure module Detector Injection valve Pump master Prime purge valve aA oP 3 sf a Pump slave Low pressure solvent valve a 35 na A Mixer Figure 3 Binary System Table3 Binary system component heights on the mast measured from the bench to the b
80. ontrolled either by an Agilent 218 Solvent Delivery Module or via OpenLAB CDS ChemStation Edition recommended When the computer controls pumps all pumps are slaves and programming is done on the computer On the pump rear panel there is a single RS 422 male connector This connector is used for bidirectional signals to and from the controller whether the controller is an external computer or another Agilent 218 Solvent Delivery Module Internal software in the Agilent 218 Solvent Delivery Module determines whether the pump is a master controller or a slave pump The possible system configurations depending on the type of pumps and controller being used are the following e Isocratic system e Gradient system with one Agilent 218 Solvent Delivery Module as the controller When several pumps are connected together the master Agilent 218 Solvent Delivery Module can control the other pumps in the liquid delivery system A master Agilent 218 Solvent Delivery Module can control up to three other slave units either three additional pumps in a quaternary system or two additional elution pumps and one injection pump The master Agilent 218 Solvent Delivery Module can control other modules in the system using outputs and receive information through input contacts All pumps are controlled by applicable instrument control software on a PC The sample inject pump is plumbed into the system with a tee The column is plumbed after the tee When s
81. oportions This is important during elution and during solvent changeover Refer to Figure 32 on page 156 for miscibility of some common HPLC solvents Acetic acid Acetone Acetonitrile Benzene Butyl alcohol Carbon tetrachloride Chloroform Cyclohexane Cyclopentane Bi immiscible Miscible Dichloroethane Dichloromethane Dimethylformamide Dimethylsulfoxide Dioxane Ethylacetate Ethyl alcohol Di ethylether Heptane Hexane Methyl alcohol Methylethyl ketone Iso octane Pentane Isopropyl alcohol V Di Propylether Tetrachloroethane Tetrahydrofuran Toluene Trichloroethane Water 3 Xylene v o 2 2 2 2 c E S 9 2 Jalc o S Lialalx 50 SIs o olL ISEE lS 3 2 Solols 2 5 alel eSis SSE lelsis fs SEBE If Se el MS lelElSESlelgla Sisk SIZ tagg fo 8 o E e 8215 5 5 8 5 SiS o 18 8 2 Jo a o o Slo SJ o s cico eVslelalelslelelelolelS cl 2l _ ecl ce lease a o o Sj je olol j to ol SlSlSla OlololL S o u 2 2 S I S18 5 S S S S S l S SiS SIS ISISLENSISISIE SSIS S195 BIC ISIE ILISJOISISlElElS Z12 9 OI x S Fe c 2Q S s 25 5 2 83181818 Sls 5 gt gt lL L E E L S S o o L e jO o 9 10 do lE S 5 L L a lo lo 66A lA 4 6 u HIG I
82. optional Cross over Ethernet cable Power cord Flow cells are required for the detector but are ordered separately See Hydraulic Connections Flowcells on page 76 for suitable flowcells Purification Solution System User Guide 29 3 Installation Delivery Checklist Autosampler The autosampler is packed in a single carton The following list shows all items delivered in a standard delivery Your personal list depends on your order therefore countercheck delivery with your order e Agilent 410 Autosampler G9331A G9332A Power cord e Agilent 410 Reservoir Rack e Standard Tray Assy G9332A Agilent and PrepStar Mast kit e Prime purge valve bracket e Prime purge valve stainless steel Delivery Checklist Fraction Collector The following list shows all items delivered in a standard delivery Your personal list depends on your order therefore countercheck delivery with your order Agilent 440 LC Fraction Collector e Assy USB RS 232 serial adaptor optional e Rack Funnel Rack Kit optional Power cord 30 Purification Solution System User Guide Installation 3 Delivery Checklist Capillary Kits Your order contains one of the following Capillary Kits Binary Pump Stainless Steel Tubing Kit 1 16 x 0 02 i d Binary Pump Stainless Steel Tubing Kit 1 16 x 0 03 i d Binary Pump Stainless Steel Tubing Kit 1 16 x 0 04 i d Binary Pump Stainless Steel Tubing Kit 1 8 x 0 08 i d Isocratic Pump Stainless Ste
83. or if one is present This tubing should be as short as possible 18 In port 1 see Figure on page 100 screw in the fitting with PTFE tubing Put the other end of the tubing into your waste container Assembling the Sample Racks CAUTION Loss of sample If a tube sits at an angle in the rack the probe may hit the side of the tube as it enters or leaves the tube This can affect the alignment of the probe and or damage the tube gt Ensure that the tubes sit vertically within the sample rack 1 Follow the manufacturer s instructions to assemble the sample racks 2 If needed Insert the overlay by placing the overlay over the top of the rack Purification Solution System User Guide 101 3 Installation Installing the Sample Racks 1 Standard racks Fit the pegs on the rack into the holes on the rack location mat The rack closest to the pillar of the fraction collector is considered rack number one OR Autosampler rack Type 128 or Type 200 Slide the extended portion on the bottom of the rack into the long slit on the rack location mat 4 Figure 22 Rack location mat 1 Cut outs on the rack location mat 2 Holes for regular tube racks 3 Holes for the autosampler rack 102 Purification Solution System User Guide Installation 3 Rack Orientation 1 Place the rack in the correct orientation to get your samples in the desired order The following image shows an example of where the fir
84. ottom of the bracket Part Height in mm in Injection valve 485 19 1 Prime purge valve 385 15 2 Low pressure solvent valve 300 11 8 Low pressure solvent valve 100 3 9 Purification Solution System User Guide 37 3 Installation Auto Preparative System Column Tee Detector Injection pump Pump A Pump B Mixer Fraction collector 38 y i 72E p Figure 4 Gradient Auto Preparative System The injection pump is used for automatic injection As all modules can be mixed there is no general proposal for stacking Depending on the modules that need to be installed there are two different ways of stacking As described in the figure above the pump can be set at the bottom of a stack and the detector at the top The other way is to build two stackings one with the pumps stacked on one stack and a detector and sampler that are stacked on a second stack Purification Solution System User Guide Installation 3 Installing the Solvent Delivery Module Electrical Setup CAUTION Damage to the module gt Use only correct fuses recommended for your voltage usage gt A label stating the operating power rating of your instrument as wired in the factory is affixed to the rear panel adjacent to the power receptacle and voltage selection assembly J1 gt The pumps are shipped with fuses installed ready for operation on 220 230 V You will need to reconfigure the voltage for 115
85. p the Software 10 Set Fraction collector 11 Click OK to confirm method Setup is completed Purification Solution System User Guide 129 3 Installation Setup the CIM in the OpenLAB CDS ChemStation PrepLC Drivers Parts required Preparations 130 Setup the CIM in the OpenLAB CDS ChemStation PrepLC Drivers The Control Interface Module CIM in the OpenLAB CDS ChemStation PrepLC Drivers can be used for acquiring signals from extern detectors If 218 SD 1 and 1260 modules are connected via remote cable error messages will be properly relayed With the remote cable it is also possible to run 218 SD 1 preparative HPLC system with any Agilent 1200 Infinity Series detector using the CIM as trigger source for fractionation For this analog input interface one or two 218 SD 1 pump modules can be mixed The CIM offers different features e from the ability to configure the module to establish communication with the module and re establish in case of communication loss e to edit the CIM detector method to perform a run and acquire data e to view the CIM status in the RC NET Status Dashboard e to activate the CIM relays manually to monitor the CIM signal to fractionate and e to detect the 440 Fraction Collector peaks on the CIM signal p n Description 5062 3378 Remote cable OpenLab CDS Chemstation Control Panel is configured as described in Setup the CIM in the OpenLAB CDS ChemS
86. r and gently pull out the needle port from the valve 3 Remove the selection handle from the valve by loosening the two grub screws securing it to the injection valve body using the provided 2 mm hex key Flat side of metal post 4 Remove the two screws and injection valve bracket from the accessories bag that came with the injection valve 5 Attach the bracket to the injection valve with the two screws Purification Solution System User Guide 49 3 Installation 6 Replace the selection handle and tighten the two grub screws securing the handle using the 2 mm hex key The grub screws should be flush with the flat side of the metal post Needle port Next Steps 7 Replace the needle port in the valve 8 Attach the injection valve to the mast For correct height see Isocratic System on page 36 isocratic system or Binary System on page 37 binary system 50 Purification Solution System User Guide Installation 3 Installing the Prime Purge Valve and Bracket_system Parts required p n Description 1 Prime purge valve consult the documentation that came with the valve for the part number 1 CP16267 Hex key 2 mm 1 R000048606 Prime purge valve bracket for stainless steel purge valve 1 R000048616 Prime purge valve bracket for PEEK purge valve While stainless steel images are shown here the procedure is the same for PEEK or Titanium prime purge valves 1 U
87. r into the flowcell body Do not over tighten as this will damage the threads Thumbscrew Outlet connection Inlet connection Tubing NOTE The position of the inlet and outlet connections varies for different flowcells 78 Purification Solution System User Guide Installation 3 3 Remove the front panel see Removing the Front Panel on page 75 for more information 4 Carefully position the flowcell so that the two thumbscrews are positioned in the threaded holes in the flowcell compartment a Push at the center of the flowcell to ensure it is positioned squarely in its housing and that it is not tilted in any way It is critical to the performance of the detector that the flowcell is inserted correctly The internal optical components of the flowcell are an integral part of the detector s optical system If the flowcell is not fitted correctly it will have an adverse effect on detector performance Purification Solution System User Guide 79 3 Installation Next Steps 5 Secure the flowcell by tightening the thumbscrews with your fingers Alternate tightening the thumbscrews until they are snug 6 Replace the front panel For optimum performance the detector should be operated with the front panel in place This is because the foam on the inside of the panel stops breezes which may cause instability and noise from reaching the flowcell The flowcell s
88. rminal strip J2 68 J3 68 troubleshooting 136 U unpacking 32 using 136 V vials filling and sealing 92 wW waste Purification Solution System User Guide Index electrical and electronic equipment 159 water and leakage drain connecting 89 WEEE directive 159 Purification Solution System User Guide 167 www agilent com In This Book This manual provides setup information on following modules Agilent 218 Solvent Delivery Module G9300A G9301A e Agilent 325 UV VIS Dual WL Detector G9309A e Agilent 410 Autosampler G9331A G9332A Agilent 440 Fraction Collector G9340A The manual describes the following e Optimizing stack configuration Setup hardware e Setup software e Setup methods Agilent Technologies 2012 2013 Printed in Germany 03 2013 G9300 90301 EE Agilent Technologies
89. rrectly per formed or adhered to could result in damage to the product or loss of important data Do not proceed beyond a CAUTION notice until the indicated condi tions are fully understood and met A WARNING notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly performed or adhered to could result in personal injury or death Do not proceed beyond a WARNING notice until the indi cated conditions are fully under stood and met Purification Solution System User Guide In this book In this book This manual provides setup information on following modules e Agilent 218 Solvent Delivery Module G9300A G9301A e Agilent 325 UV VIS Dual WL Detector G9309A e Agilent 410 Autosampler G9331A G9332A e Agilent 440 Fraction Collector G9340A 1 Introduction This chapter introduces to the Agilent 218 Purification Solution system and its components 2 Site Requirements and Specifications This chapter provides infromation on site requirements and specifications of your system 3 Installation This chapter gives information about the installation of your Agilent 218 Solvent Delivery Module Agilent 325 UV VIS Dual WL Detector Agilent 410 Autosampler and Agilent 440 Fraction Collector 4 Using Troubleshooting Maintenance and Parts This chapter provides information on how to access further details on the system components 5 Cables This c
90. st sample is delivered to and the orientation of the regular racks provided with the fraction collector First sample dispensed in this position rack 1 tube 1 Rear of the fraction collector next to the 500000000006 pillar OOO0O0O00000000 OO0OO0O000000000 OOOOQOQ00Q000000 OOO0O000000000 OO0OO0000000000 OOO0OO0O00000000 Front of the fraction collector Figure 23 Tube positions on the fraction collector Purification Solution System User Guide 103 3 Installation Connecting the Power to the Fraction Collector The connection panel of the Agilent 440 Fraction Collector G9340A is located on the pillar also called a control column The panel contains an I O port indicators DIP switches the power socket and switch and a RS 232 port for communication between the Agilent 440 Fraction Collector G9340A and the system or computer that is running instrument control software Error indicators mo mmmn 1 0 port Relay status indicators showing the status of r lays A B and 0 which are used forthe hardwired 1 0 circuit bo rd Console i software contiguration Status indicators 2 uxilliary communication port en not supported bx a Instrument pease communication port Power suppl inlet switen and fuses Figure 24 Connection panel Prerequisites Three power cables are supplied with the module Select the correct one for your location WARNING Electrical sho
91. t WARNING RISK OF ELECTRIC SHOCK DISCONNECT POWER BEFORE SERVICING AVERTISSEMENT RISQUE DE CHOC ELECTRIQUE COUPER L ALIMENTATION AVANT LA MAINTENANCE AVERTISSE REMP LACEZ FUSE COMME INDIQU WARNI REPLACE ING RISK OF FIRE E FUSE AS MARKED MENT RISQUE DE FEU Figure 19 Rear View of the autosampler Purification Solution System User Guide 3 87 3 88 Installation 1 Fuses and voltage selector Main input 3 Main switch 4 P1 I 0 connector 5 P2 1 0 connector 6 RS 232 communication interface connector 7 CE mark 8 Fan only if optional Peltier tray cooling option is installed Installing the Sample Tray 1 Place the sample tray in the autosampler and rotate it until the tray drops into the slot only one position is possible 2 Turn the tray fixation knob clock wise to fixate the tray see Overview of the Module on page 85 for an overview Connecting the Waste Tubings The following Waste Tubings must be connected to the autosampler General waste tubing e Syring waste tubing e Water and leakage drain Connecting the General Waste Tubing 1 Connect the drain tubing to the right hand drain hose connector of the autosampler and put it in a bottle which is placed on the floor All the liquid dispensed while the autosampler is in the wash position is removed through this
92. tation PrepLC Drivers on page 130 Purification Solution System User Guide Installation 3 1 Select Configure Instrument PEO ER R Edit Delete Refresh Lock Create Configure Edit Notifications Shortcuts Instrument Instruments and Locations Properties Actions Navigation PrepLc Not Connected EEEE Instruments Start Instrument X Prepic iann Launch omne EE instruments X Administration Your startup license expires in 60 days 2 Select No to avoid the auto configuration It doesn t work with this version peers Z 20 woas evaluation T Enable rteigert Repoting Configurable Mosses Selected Moses Agilent LC Modules and System e Ato d Fracton Colector d Congr sha tow Pow Fraction Colector iroa 2 HP Sampler Ors trony cergetin f I 4 2 Low Row HP Sampler There s ne configuration salable for this instrument De you want to i My vacrccnteyret Ask tor configuration charge at Chem Staten satup Addtoral configuaton x Cancel Hep Purification Solution System User Guide 131 3 Installation 3 Select the 218 SD 1 CIM on the left panel of the Configurable Modules Agilent LC Syst len tlem g ys TI 3D spectral evaluation Use classic drivers VE Intelligent Reporting Method load on startup Always ask user to choose an option Zz Configurable Modules Selected Modules Agilent LC Modules and Systems Agilent LC Modules and Systems Auto e 410 Autos
93. the Agilent 325 Detector The only line installed by the user where dead volume and low holdup are critical is the line from the column exit to the flowcell inlet port This line should be as short as possible The Agilent 325 Detector can be fitted with any one of four flowcells ordered separately Each one has an inlet and outlet connection and quartz optics cell window Your chosen flowcell type is packed internally in the detector The four flowcell types are outlined in Table 8 on page 76 Table8 Compatible flowcells Flowcell type Flowcell p n pathlength Column ID Flow rate Maximum pressure Analytical 210181800 9mmxO0mm 2 8mm 0 0001 69 bar 10 mL min 1000 psi Preparative 210181900 9mmximm 4 76mm 1 69 bar 500 mL min 1000 psi Scale Up 210224200 4 mmx 4 76 mm 10 69 bar 0 25 mm 200 mL min 1000 psi Super Prep 210182000 4 mmx 8 152 mm 30 69 bar 0 15 mm 1200 mL min 1000 psi Micro analytical 210182100 4mmxOmm 1 4mm 0 69 bar 20 mL min 1000 psi A pathlength of a mm x b mm means that the sample light path has a pathlength of a mm and the reference light path has a pathlength of b mm A reference light path of zero means there is no fluid in the cell air acts as the reference Extended range cell It is important for the extended range cells to run at least at the minimum flow rate 76 Purification Solution System User Guide Installation 3 Installing a Flowcell The Agilent 325 UV VIS Dual Wavelen
94. the instrument Figure 17 Installing the Agilent 325 Detector door Installing and Removing the Door 1 Gently push down on the door and slide the lower hinge pin out of the lower hinge 2 Lift and slide out the top of the door Purification Solution System User Guide 83 3 Installation Installing the Autosampler Location of the Autosampler Module The best place to install your Agilent 410 Autosampler is at the top of the module stack This stack would normally be solvent delivery module on the bench detector on top of the SDM and finally the AutoSampler From the right hand side you have the shortest connection to the injection valve of the Agilent 410 Autosampler to the column 84 Purification Solution System User Guide Installation 3 Installing the Autosampler Overview of the Module 7 6 8 5 9 4 10 3 11 12 2 13 Figure 18 Autosampler overview Purification Solution System User Guide 85 3 86 Installation wo 10 11 12 13 Vial wash drain Condensed water and leakage drain Tray fixation knob Sample tray Syringe dispenser Keyboard Buffer tubing Needle arm Injection valve Oven compartment Tubing guide Wash position Position for transport solvent and reagent vials The rear view of the autosampler is shown in Figure 19 on page 87 Purification Solution System User Guide Installing the Autosampler Installation 4 l
95. tion Solution System User Guide Index 3 3 way pump head prime valve installation 52 A AC power 73 Agilent oninternet 164 analog output 140 assembling fraction collector 96 autosampler connecting 92 lifting 34 location 84 service 13 B battery safety information 160 C cables other 72 power 72 checklist delivery 29 pump 29 columnoven 13 communication cabling connecting 66 components 95 condensation 20 connections workstation control 139 connector external contacts 67 connectors external 67 D defect on arrival 28 delivery checklist autosampler 30 capillary kits 31 detector 29 fraction collector 30 delivery 26 detector outlet back pressure restrictor 81 driver installation 115 E electronic waste 159 F features 11 flowcell types 76 flowcell install 77 G general waste tubing connecting 88 Purification Solution System User Guide Index hardware setup 110 high pressure tubing pump head 58 high pressure hazard 155 inlet filter assembly 56 inspection 32 installation internal mixer 45 mast kit 47 overview 96 pressure module 43 probe 98 pumphead 41 tubing 98 installing door 82 internal mixer installation 45 internet 164 introduction autosampler 12 detector 11 fraction collector 15 preparative autosampler 14 system 8 L lithium batteries 160 location 165 Index detector module 71 low pressure tubing 56 M maintenance module 136 anu
96. tion is subject to the following two conditions This device may not cause harmful interference e This device must accept any interference received including interference that may cause undesired operation If this equipment does cause harmful interference to radio or television reception which can be determined by turning the equipment off and on the user is encouraged to try one or more of the following measures e Relocate the radio or antenna e Move the device away from the radio or television e Plug the device into a different electrical outlet so that the device and the radio or television are on separate electrical circuits e Make sure that all peripheral devices are also certified e Make sure that appropriate cables are used to connect the device to peripheral equipment e Consult your equipment dealer Agilent Technologies or an experienced technician for assistance e Changes or modifications not expressly approved by Agilent Technologies could void the user s authority to operate the equipment Purification Solution System User Guide Appendix 6 ICES NMB 001 This ISM device complies with Canadian ICES 001 Cet appareil ISM est conforme a la norme NMB 001 du Canada Purification Solution System User Guide 163 6 Appendix Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at http www agilent com 164 Purifica
97. uch an injection occurs the Thru Pump Injector automatically begins to ramp up to the correct flow rate delivers the sample and then ramps back down to ensure consistent flow rate at all times during the analysis The Solvent Delivery Modules work with the Thru Pump Injector and lower their flow rate during the injection to maintain a constant overall solvent flow rate e Gradient system with HPLC control software as a controller In this configuration all pumps are slaves and the computer is the system controller The HPLC control software controls the pumps via the serial interface cable and other devices through contact closures on the Control Interface module CIM built into the Agilent 218 Solvent Delivery Module Purification Solution System User Guide Introduction 1 Introduction to the Agilent 325 UV VIS Dual Wavelength Detector The Agilent 325 UV VIS Dual WL Detector is integrated into a Liquid Chromatography System The detector is controlled remotely by OpenLAB through Ethernet communications In this situation all functions of the detector are controlled through the Workstation software The detector measures the sample absorbance at the user selected wavelength The absorbance is displayed Wavelength absorbance parameters are time programmable Features of the Agilent 325 UV VIS Dual WL Detector e Stackable module Interchangeable flowcells Simple lamp replacement e Comfortable control OpenLAB e Wide detection ra
98. ure setting is not adjustable If the restrictor fails or becomes plugged replace the existing cartridge with a new 2 76 bar 40 psi replacement Purification Solution System User Guide 81 3 Installation Installing the Door The module door may be attached to the front of the Agilent 325 Detector to cover the tubing connections to the flowcell If the Agilent 325 Detector is the top module in the stack the door cap should be installed before installing the door on the module Installing the Door Cap If the Agilent 325 Detector is not at the top of the stack do not install the cap and proceed to the instructions describing door installation 1 Stand the door upside down on a flat surface i e with the two ribs towards the bottom Ribs Magnetic latch Door cap Figure 16 Installing the door cap 2 Take the cap and remove the protective paper exposing the adhesive that will attach the cap to the door 3 Insert the cap into the door and press the adhesive onto the inside of the door lip Be sure to keep the door edges and cap edges flush 82 Purification Solution System User Guide Installation 3 Installing the Detector Installing the Door 1 Insert the top hinge pin into the top hinge 2 Gently press down on the top of the door and slide the lower hinge pin into the lower hinge The door should now pivot on the pins and close The magnetic door latch should stick to
99. utomated HPLC system controlled by an external computer In each case the Agilent 218 Solvent Delivery Module provides outstanding accuracy over its entire range of pressures flow rates and solvents The Agilent 218 Solvent Delivery Module operates very quietly because of minimal motor noise and resonance vibrations A complete Agilent 218 Solvent Delivery Module includes a drive module a pump head and a pressure module One of the Agilent 218 Solvent Delivery Modules in the HPLC system needs to have a pressure module installed in its compartment in the pump side panel The pressure module dampens pulsations and supplies the current system pressure value to the drive module Software in the drive module ensures that the system pressure is within pre set maximum and minimum limits Flow rates are automatically corrected for solvent compression effects based on the system pressure value read from the pressure module and a compressibility factor entered by the user for each solvent The Agilent 218 Solvent Delivery Module operates with a variety of Agilent 218 Solvent Delivery Module heads to maintain specified performance over designated flow and pressure ranges The easily replaceable pump heads are Purification Solution System User Guide 9 1 10 Introduction self contained units including a spring loaded piston and check valve cartridges Pump heads are not included with individual drive modules A complete HPLC system can be c
100. which is usually built into the motherboard or with an Ethernet network card installed However if you have a PC that has no network interface you will need to install and configure a Network Interface Card PCI bus The PC must have a spare PCI slot for the installation of this device You are also responsible for setting up and maintaining any LAN configuration where a detector may be used All network issues are to be dealt with by the user Purification Solution System User Guide Cables 5 Synchronization Signals Synchronization Signals The synchronization signals at P9 and J10 are used to synchronize the operation of a group of instruments that are not interfaced to OpenLAB The synchronization signals come in four pairs and define how the detector will operate in a HPLC system These signals are important for controlling timing and synchronization of the detector with the other devices in the system Synchronization signals are closely tied to the detector states and transitions P9 and J10 pin designations are shown in Figure 29 on page 145 J10 P9 6 Start Out 15 5 Volts 1 Fault Out 8 Ground 7 Not used 14 Lamp Off 2 Fault Out 7 Lamp Off 8 Ground 13 Auto zero 3 Ready In 6 Auto zero 9 5V 12 Start In 4 Ready In 5 Fault In 11 Enable Out 5 Start Out 4 Fault In 10 Enable Out 3 Ready Out 5 Vi 2 Ready Out lata 1 Start In Figur

Introduction to the Agilent 218 Solvent Delivery Module

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