GenTarget`s EcoTMPlasmid DNA Miniprep Kit User Manual
Contents
1. 7930 Arjons Drive Suite B San Diego CA 92126 Phone 858 6788683 Fax 800 3804198 Email orders gentarget com GenTarvet Ine How to select the lentivirus products Product Selection Guideline for GenTarget s lentivirus Lentivirus Promoter Antibiotic Fluorescent Lentivirus category types marker marker formats Target expression suCMV Puromycin P Regular lentivirus in Fluorescent Optional Tet Blasticidin DMEM markers inducible l medium promoter Neomycin Concentrated PBS Common enzumes Knockdown shRNA CAG promoter Or No any antibiotic Ultra titer microRNA Tissue or Pathway marker lentivirus in specific promoter IRFP PBS Anti miRNA For search GenTarget s target over expression lentivirus You can search a product in the search field by input Search by gene name for example NR2E3 Search by gene Alias names or gene_synonym alternative names for example PNR Search by the gene s transcript mRNA ID as NM_xxxxxx for example NM_014249 Or you simply open this Product Manual for all available over expression lentivirus for human mouse or rat genes GenTarget provides all kinds of ready to use lentivirus products Many cases even for the same target there are multiple products many catalog numbers to select It seems a little confusion and hard to select which product to use The following guidelines can help you narrow down the products for your2. Lentivirus product selection guideline Page 3 of 3 www gentarget com GenTarget Inc 2015
3. need Lentivirus product selection guideline Page 1 of 3 www gentarget com GenTarget Inc 2015 7930 Arjons Drive Suite B San Diego CA 92126 Phone 858 6788683 Fax 800 3804198 Email orders gentarget com GenTarvet Ine 1 Lentivirus category First you go for the lentivirus catagories or go for a sepcific target marker GenTarget provides lentivirus for Fluorescent markers enzyme specific gene over expression or knockdown shRNaA microRNA anti miIRNA and others Each type of lentivirus has a general product manual In that manual you may see multiple products with different features for the same target For example Luciferase product manual or Fluorescent protein manual Then you select the product based upon each product features listed below Promoter suCMV inducible CMV tetCMV EFla or CAG promoter GenTarget provide multiple products for the same target driven by different promoters suCMV tetCMV EFla or CAG You can pick the product driven by your desired promoter In some products the promoter can be a tissue specific or pathway specific promoter The suCMV promoter is the strongest promoter in most cell types for the highest over expression The tetCMV promoter is an optional inducible promoter it became the inducible promoter only when its repressor tetR is present The EFla promoter was modified with less tissue cell type specificity also no promoter silencing effect
4. g curve in each cell type which have to be tested prior to the antibiotic selection Puromycin and Blasticidin has the fast killing feature often in about one week Most cell types are extremely sensitive to puromycin So you select the product with the antibiotic marker good for your positive cell selection Each fluorescent marker has different wavelength Ex and Em The fluorescent maker provides an easy transduction efficiency check under Fluorescent microscope or cell selection via FACS sorting You pick the product having your desired fluorescent marker All fluorescent markers in GenTarget s products are engineered with the strongest signal intensity Lentivirus formats GenTarget s lentivirus provides in three formats You pick the virus depends on your application 1 Regular lentivirus provided in DMEM medium with 10 FBS and pre added polybrene 10x It satisfies most application Simply add the virus into your cell culture 2 In vivo ready lentivirus provided in PBS solution with higher titer It can be used for the cell types having low transduction efficiency hard to infected cell types or used for serum sensitive cell culture like stem cell or some primary cells 3 Ultra titer lentivirus concentrated virus with ultra high titer gt 10 IFU ml provided in PBS It best fits for in vivo manipulation for direct injection gene therapy research and application that requires extreme high titer lentivirus
5. s during long term cell culture thus is active in all cell types The CAG promoter has strong activity in embryonic cells some neuron and some types of stem cells If you do not have promoter preference simply pick the products with CMV or EFla promoter Selection marker antibiotic marker and fluorescent marker One important feature of lentivirus is that lentivirus provides long term expression knockdown because it integrates into cell s genome To select the positive transduced cells lentivirus often contains a fluorescent marker for cell sorting or and an antibiotic marker for killing selection GenTarget s products are made with different antibiotic selection marker Blasticidin Bsd Puromycin Puro Neomycin Neo or Hygromycin Hygro Some products also contain a fluorescent marker GFP RFP BFP CFP or YFP and sometime an luminance marker luciferase Depend your preference with the selection marker you pick the product containing your desired antibiotic marker Bsd or Puro or Neo or Hygro Lentivirus product selection guideline Page 2 of 3 www gentarget com GenTarget Inc 2015 7930 Arjons Drive Suite B San Diego CA 92126 Phone 858 6788683 Fax 800 3804198 Email orders gentarget com GenTarvet ine If you need the product with a fluorescent marker as well you pick the product with both antibiotic marker and fluorescent marker as your desired Each antibiotic has different killin
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