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1. 998 2300 Rev H Page 7 C3 Submersible Fluorometer Computer Integration Cable C ray Towed Deployment Body yellow heat shrink Flow Cap Battery Bracket Shade Cap in y Air Purge Slots pa Booster Kit Booster 1 Locking Solid Standard Insert sleeve Booster 2 Secondary Solid Standard 998 2300 Rev H Page 8 C3 Submersible Fluorometer 2 3 Identification Sensors are marked with numbers 1 2 or 3 located adjacent to the optics on the face of the C3 Submersible Fluorometer These numbers correspond to channels 1 2 and 3 in C Soft C3 software Note Channel names can be modified using C Soft Light emitting diode color table Chlorophyll a Blue Turbidit Phycocyanin Phycoerythrin Fluorescein Blue See important note on page 5 Rhodamine CDOM Refined Oils and Fuels Crude Oil Ultra Violet No Color PTSA Ultra Violet No Color Do not look directly at the optics Ultraviolet light can be damaging to the eyes 998 2300 Rev H Page 9 C3 Submersible Fluorometer 3 C Soft Windows User Interface The C Soft Windows based user interface allows for intuitive calibration data logging set up and download functions 3 1 Software Requirements and PC Installation 3 1 1 Minimum PC Requirements 4 133MHz microprocessor 600 X 800 VGA resolution Microsoft Windows 98 32 MB RAM 3 1 2 Software Installation 1 2 Exit all Windows programs
2. Depth values will now be scaled to the offset selected 3 4 Log Setup Screen The Log Setup screen allows the user to configure a logging session set the mechanical wiper to its home position and enable logging The C3 Submersible Fluorometer can log just over 64 000 lines of data Enter a log start date MM DD YYYY and time HH MM SS Enter the sampling interval HH MM SS The minimum sampling interval is 1 second Log Start 06 16 08 Sampling Interval HH MM SS Ifthe mechanical wiper is installed click the Wiper box to enable Select the number of revolutions from the dropdown box 1 10 revolutions All sensors will be wiped at specified revolutions before each measurement Note If users select revolutions that exceed sampling interval a warning message will be displayed Note Do not click the Wiper Box to enable wiper if the wiper is not installed 998 2300 Rev H Page 18 C3 Submersible Fluorometer C Sofk Manager Ea The sampling interval is too short for the number of wiper revolutions You must choose a longer sampling time less revolutions or turn the wiper off Sampling Maximum Interval of Revs 1 10 11 20 21 30 31 40 41 50 51 60 00 Be Of E to hoe 0 Cancel After log set up is complete select Enable Datalog see section 3 3 Enabling Data Output Ready State A prompt will appear asking if user is ready to enable logging selec
3. Insert the C Soft CD also located on the Turner Designs website on the Support page 3 Open the C Soft software folder and double click on the setup icon The software will automatically be installed in the PC s C Program Files C Soft folder unless user selects alternate location After restarting the PC double click on the C Soft icon located on the desktop J E ft C 5oft C Soft Note You may receive a warning message asking if you want to unblock messages select Unblock 3 1 3 PC Software Interface 1 Connect the 9 pin serial end of the interface cable to the serial port on the back of the PC Note If your computer only has USB ports we recommend purchasing a USB to serial adapter cable htip www belkin com search for Part Number F5U409v1 Connect the 8 pin female end of the interface cable to the C3 Submersible Fluorometer s 8 pin male connector Connect the interface cable s 12V pin to the 12V power supply Plug the 12V power supply into a power source 5 Wait for initialization process to complete 998 2300 Rev H Page 10 C3 Submersible Fluorometer i x Initializing Unit The connection icon located on the upper right of the screen will turn green if connected Note If the connection icon does not turn green disconnect and reconnect power File Settings COM Pork window Help Connected 3 2 Software Operation 3 2 1 Settings Screen The Settings Screen allows
4. 53 8 76 11 64 8 76 9 66 19 12 lif22 10 10 06 54 8 28 11 40 6 92 9 66 19 12 lif22 10 10 06 55 8 20 11 92 7 16 9 66 19 12 llf22 10 10 06 56 7 80 11 84 5 96 9 72 19 12 dieli Mee at a Eonnect Disconnect 3 Log Interval Seconds e Choose Shapefile to log to using Set Log File Button form can now be closed 998 2300 Rev H Page 30 e Open Show Live C3 Data form which shows larger version of C3 data along with GPS data and Port Logging Status F Depth im Channel 1 PortOpen 9 66 5 52 52 U3 status 63 Data Valid Log Status Not Logging Temp C Channe 2 Log File 19 16 11 1 Latitude DD ddd Time Channel 3 41 258 76 Longitude DOD dddd 10 11 03 6 6 173 08170 l 173 08170 e Start stop logging to file using toolbar buttons Log Data To File Starts Logging to file based on Log interval CE Stop Pause Logging to File Stops Pauses logging Adds Flag to current data point and gets user notes on flag ad Pigg Eater Oln e g Bad reading C3 out of water or Start Transect 3 xi Add Flag Notes Cancel Data Point Note that flag only commits point once the OK button is pressed on user input Long delays in writing notes may influence location of flagged point 998 2300 Rev H Page 31 e Logged data has the following fields Chan1_ Val Channel 1 value Chan1_ Unit PPB Channel 1 Units from C3 Setup Form Not C Soft Chani_Type Channel 1 Name from C3 Setup Form No
5. are labeled 1 2 or 3 adjacent to the optics on the face of the instrument Each channel is factory set in the C3 Windows Software Note The channel labels can be modified in the C Soft software Recommended Measurement Practices The following steps will improve the accuracy and repeatability of your measurements especially at low concentration levels 1 Use a dark plastic or glass container for your standards and or samples Note Some plastics may fluoresce and might interfere with measurements If using a glass beaker place it on a non reflective surface preferably black Ensure that the sensor is gt 3 inches above the bottom of the container Ensure that the sensor is in the center of the container and has gt 2 inches clearance between the sensor and the inside surface of the container 5 If using a glass beaker check if the optical surface of the sensor is free of air bubbles Remove air bubbles if any are observed If using a dark plastic container run the wiper or your finger over the optical sensors to ensure the sensor heads are free of air bubbles Calibrated Sensor gt 2 inches all round Glass Beaker 998 2300 Rev H Page 25 Appendix C C3 Submersible Fluorometer Wiring and Cable Guide The C3 Submersible Fluorometer outputs digital data in three formats 1 Digital data that can only be read by C3 Submersible Fluorometer s software 2 Digital data in ASCII format that can be read by larger mu
6. locking nut should be screwed down so that the adjustment screw is held firmly in place Note The response of every solid standard is unique Hence a new correlation must be determined for every sensor 8 Now your Solid Secondary Standard has been correlated to a known concentration and as long as it is no longer adjusted and is stored and used properly that 998 2300 signal will remain a stable reference point 9 For comprehensive information on fluorescence please refer to the application section of Turner Designs website Solid Standard Table Application Solid Standard Chlorophyll a Yes Phycocyanin Yes Phycoerythrin Yes Rhodamine Yes Fluorescein Yes Solid standards are not available for Turbidity or Ultraviolet sensors Installation C3 with wiper Remove the tightening nut and wiper arm from the C3 Tightening nut Now follow steps 1 4 of the installation instructions Ordering Instructions Solid Standard Cap amp 1 Solid Standard Individual Solid Standard Rev H Page 39 Appendix K C Series Analog Adapter Introduction The C Series Analog Adapter P N 2300 480 allows users to convert the standard digital output from the C3 Submersible Fluorometer to a 0 5 VDC analog signal The Adapter can convert the signal from 1 2 or 3 channels installed on a C3 The voltage output is proportional to the fluorescence intensity measured by the sensors Modeled after the C
7. the Mechanical Wiper NNORUCHON 7 Hand tighten nut to secure wiper 450 for the C3 Submersible Fluorometer is designed to reduce Note do not use any tools to bio fouling during extended tighten the tightening nut deployments Benefits e Helps maintain consistent readings by reducing bio fouling e Replacement brushes available P N 2300 460 Installation 1 Connect the C3 to a computer that has C Soft software and supply g power 9 Ifthe wiper does not come to rest 2 Start the C Soft software in the desired position unscrew 3 Click on the Log Data tab the tightening nut remove the i ly 4 Click the Test Wiper button to eel see mba ime Pest SiePe 6 8 bring the motor shaft to its home position Replacement Brush Installation Click Test Wiper 5 Install O ring into motor shaft AS groove Note Do not lubricate N O ring Wiper hub 6 Slide the wiper assembly onto the i motor shaft with brushes facing towards the optical head and position the brushes between two Fa Sensors sV Arm locking screw C 1 Using the Allen Wrench provided loosen the arm locking screw 2 Pull out the brush arm with brush 3 Slide the brush towards the open end of the brush arm to remove the spacer and the brush 4 Slide in a new brush Slide in the spacer p Brush arm ps a e i Brush gt S i Spacer Sensors 5 Position the brush arm onto the wiper assembly and t
8. 0 00 21 47 gt 06 16 08 10 34 59 70 24 72 20 0 00 21 47 Chan 1 Rhodamine 80 44 __Calbrate e 06 16 08 10 35 00 70 40 7472 o0 21 48 06 16 08 10 35 01 68 92 74 28 0 00 21 48 Calibrate 06 16 08 10 35 02 67 92 78 28 0 00 21 47 06 16 08 10 35 03 71 28 73 20 0 00 21 47 Gana Phycocyanin F208 Galhrate 06 16 08 10 35 04 71 76 7 04 0 00 21 48 06 16 08 10 35 05 73 88 77 00 0 00 21 47 Ds t6 06 1035 06 75 24 7624 0 00 2147 06 16 08 10 35 07 72 48 0 00 21 47 06 16 08 10 35 08 73 76 0 00 21 47 Ss o6 16 08 10 35 09 7448 7960 Jooo as E E 06 16 08 10 35 10 76 28 0 00 21 47 Calibrate 06 16 08 10 35 11 75 68 0 00 21 47 06 16 08 10 35 12 72 12 0 00 21 48 Depth meters o Calibrate 06 16 08 10 35 13 73 60 0 00 21 47 06 16 08 10 35 14 72 64 0 00 21 48 Temp C 215 06 16 08 10 35 15 75 40 7124 0 00 2147 06 16 08 10 35 16 74 00 70 60 0 00 21 48 06 16 08 10 35 17 76 44 73 20 0 00 21 48 osjo 1035 18 7464 7816 om0 2148 06 16 08 10 35 19 76 32 74 24 0 00 21 48 06 16 08 10 35 20 78 08 75 12 0 00 21 48 view Calibration Data 06 16 08 10 35 21 79 04 7228 000 21 48 E 06 16 08 10 35 22 81 48 74 76 0 00 21 48 06 16 08 10 35 23 70 84 78 52 000 21 48 06 16 08 10 35 24 76 16 74 60 0 00 21 48 06 16 08 10 35 25 81 36 73 28 0o00 21 48 Start Data Capture 06 16 08 10 35 26 80 52 72 92 0 00 21 48 06 16 08 10 35 27 80 56 75 68 0 00
9. 21 48 06 16 08 10 35 28 78 60 78 36 0 00 21 48 06 16 08 10 35 29 80 44 76 80 0 00 zae T a start chelsea Donova 9 Manual and Inst untitled Paint C Soft Manager 7 Microsoft Word 8 Go ws A 3 3 N UE 10 35 AM Waveform Screen Users can graphically view real time data for each sensor by clicking the Display Waveform box Channel names and units will be displayed on the Y axis Time and date will be displayed on the X axis Clicking the Autoscale box will automatically scale graphs Users may also rescale graphs by unchecking the Autoscale box and manually entering desired values for either Y or X axis ranges Data can be saved during waveform mode by clicking Start Data Capture Data files will be saved as csv format File Settings COM Port Help C6 Multi Sensor Platform Connected Data Log Chart Display Waveform V Autoscale IV Settings Current Data Log Setup Download Data h hid Ap ane puny Current Data Chan 1 Chlorophyll a 15 52 A Chan 2 Brighteners 14 28 A Es Chan 3 Fluorescein 39 76 Chan 4 CDOM 12 8 f Chan 5 Phycocyanin 65 64 i JE Chan 6 Of 23 6 Depth meters 0 Calbrate Temp C 23 1 View Calibration Data Start Date Capture 998 2300 Rev H Page 13 C3 Submersible Fluorometer Calibration Screen Users can calibrate sensors in either the direct concentr
10. 3 Submersible Fluorometer s battery pack the Analog Adapter is designed to connect directly to the C3 The C3 Battery Bracket P N 2300 603 is required to hold the Analog Adapter in place and secure it preventing any break in communication or power With the C Series Analog Adapter users can integrate their C3 Submersible Fluorometers into any CTD or multi parameter platform that will accept a 0 5 volt output signal Required Accessories The following accessories are required for installation and operation of the Analog Adapter Battery Bracket PN 2300 603 Designed to hold the Analog Adapter in place and support it to avoid any break in communication or power ensuring continuous data output An Analog Pigtail Cable We offer various lengths that are necessary for standard integrations P N 2100 750 2 foot pigtail cable P N 2100 755 5 meter pigtail cable P N 2100 751 10 meter pigtail cable P N 2100 752 25 meter pigtail cable P N 2100 753 50 meter pigtail cable C3 Submersible Fluorometer Setup There are only a few steps required to set up the C3 Submersible Fluorometer for analog output There are two methods that may be used for calibrating C3 channels sensors The first is a Raw Fluorescence Units Blanked Calibration refer to section 3 3 2 in the C3 s user manual and the second is a Direct Concentration Calibration refer to section 3 3 1 in the C3 s user manual Use the Raw Fluorescence Units B
11. TURNER DESIGNS Reliable Instruments for an Unreliable World Submersible Fluorometer User s Manual September 19 2011 P N 998 2300 Revision H TURNER DESIGNS 845 W Maude Ave Sunnyvale CA 94085 Phone 408 749 0994 FAX 408 749 0998 C3 Submersible Fluorometer Table of Contents 1 Introduction 1 1 Introduction 1 2 Optical Sensors 1 3 Important Note Regarding the Use of the Fluorescein Sensor 2 Inspection and Setup 2 1 Instrument Checklist 2 2 Optional Accessories 2 3 Identification 3 C Soft Windows User Interface 3 1 Software Installation and PC Requirements 3 1 1 Minimum PC Requirements 3 1 2 Software Installation 3 1 3 PC Software Interface 3 2 Software Operation 3 2 1 Settings Screen 3 2 2 Current Data and Calibration Screens 3 3 Calibration Procedures 3 3 1 Direct Concentration Calibration 3 3 2 Raw Fluorescence Calibration Blank Subtracted 3 3 3 Raw Fluorescence Calibration 3 3 4 Depth Calibration Optional Accessory 3 4 Log Setup Screen 3 5 Enabling Data Output Ready State 3 6 Downloading and File Management 4 Maintenance and Warranty 4 1 Maintenance 4 1 1 Rinsing 4 1 2 Care for the Bulkhead Connector 4 2 Warranty Terms 4 3 Warranty Service 4 4 Out of Warranty Service Appendices C3 Submersible Fluorometer Specifications C3 Submersible Fluorometer Recommended Practices C3 Submersible Fluorometer Wiring and Cable Guide Linear Range Quenching and Temperature Considerat
12. Time Cancel Set Unit Clack 3 2 2 Current Data and Calibration Screen 998 2300 The Current Data Screen allows users to view real time data acquisition in both tabular or waveform display as well as conduct calibrations for each channel Current Data Channel names are displayed but cannot be changed in the Current Data screen Real time values are displayed next to each channel and in the adjacent Data Table A calibration button is available for each channel and the depth sensor Real time data can be saved by selecting the Start Data Capture button Users will be prompted to enter a file name and file location Data files will be saved as csv format Tabular Screen Real time data including date time units and temperature are continuously scrolling on the tabular screen for enabled channels Channel names will appear in the associated column headings If units are not selected during calibration a relative fluorescence units RFU label will appear Rev H Page 12 C3 Submersible Fluorometer ii iai x File Settings COMPort window Help Connected e Data Table Display Waveform I z Date Time Rhodamine Phycocyanin Depth Tem Settings Current Data Log Setup Download Data i REU nal Y rare ae p l Current Data 06 16 08 10 34 57 69 08 76 72 0 00 _ 21 47 06 16 08 10 34 58 69 72 76 64
13. ample it can be used for future 3 Align Solid Standard Cap numbers calibrations The Solid Standard can with C3 sensor head numbers and also be used as a stable reference snap on the Solid Standard Cap point for checking signal stability and or instrument drift Benefits e Adjustable for a range of concentrations 2 e Can be used for quick and easy wt correlation e Can be used for multiple sensors e Set screws ensure accurate positioning Solid Standard Cap numbers Features 4 Slightly rotate cap until it is set into position Note You will feel hear a click when cap Is positioned 5 Insert up to 3 Solid Standards into the desired optical port position of the Solid Standard Cap gt Numbered Positioning Positions Screws 998 2300 Rev H Page 38 6 Rotate the Solid Standards until they set into position Note You will feel hear a click when the Solid Standard is positioned 7 Adjust the Solid Standard to produce the same fluorescence output value from the sensor as in step 1 To adjust each solid standard use the green screwdriver to unscrew the locking nut as far as it will go CON Adjustable i Locking Screw To change the signal level insert the green screwdriver through the hole in the locking nut Rotate it until it engages with the adjustment screw that is beneath the locking nut The signal can be adjusted to desired reading by turning the screw Once the desired reading has been obtained the
14. assumes 0 and 35PSU salinity Depth Dialog vi 7 E Ioj x Channel Calibration Unit Label Depth Current Pressure 0 00 PSI Step 1 Press Set Zero to set the zero Reading atmospheric zero Set Zero Step 2 Select a pressure compenstion Atmospheric offset only method Atmospheric and gravity offset Sten 3 When you are done press Save Calibration or press Cancel at Save Calibration Cancel any time to abort this calibration Atmospheric Offset Step 1 Method 1 Select the Calibrate button for depth in the Current Data screen Pressure in PSI will be displayed in the Current Pressure window After Current Pressure readings are stable at desired location select Set Zero Step 2 Method 1 Select Atmospheric offset only Step 3 Method 1 Select Save Calibration button Depth values will now be scaled to the offset selected Rev H Page 17 C3 Submersible Fluorometer Atmospheric and Gravity Offset Step 1 Method 2 Select the Calibrate button for depth in the Current Data screen Pressure in PSI will be displayed in the Current Pressure window After Current Pressure readings are stable at desired location select Set Zero Step 2 Method 2 Select Atmospheric and gravity offset Enter the latitude Step 3 Method 2 Select Save Calibration button
15. ation mode raw fluorescence mode or blank subtracted raw fluorescence mode O x Channel Calibration _ A Unit Label Chan 1 Rhodamine Current RFU 93 20 Step 1 Press Set Blank to set the blank Blank RFU value to the current RFU x1 Set Blank x10 x100 Step 2 Enter the value of your standard Standard Value idl and select the units of measure Step 3 Press Set Standard to set the Standard RFU standard value to the current Set Standard RFU Step 4 Optional Click the temperature Temp Compensation compensation box to activate 5 Step 5 After calibration is complete press i Save Calibration Save Calibration Cancel Click to use uncalibrated Raw Fluorescence Mode Direct Concentration Mode Values reported in the Direct Concentration Mode are scaled to a predetermined standard value and blank subtracted The equation used to calculate concentrations after calibrating to Direct Concentration Mode iS Standard Value units x Sample RFU Concentration units standard RFU Raw Fluorescence Blank Subtracted Mode Values in the Raw Fluorescence Blank Subtracted Mode are noted as RFUB It is important to note that these values are blank subtracted but are not scaled to a standard they are relative values Raw Fluorescence Mode Values in the Raw Fluorescence Mode are referred to as Relative Fluorescence Units RFU It is important to note that these values are not blank subtracted or sca
16. d select the Set Standard button Step 4 Optional Temperature Compensation Temperature compensation is optional it enables the C3 to compensate for changes in fluorescence due to varying temperatures as detected by the C3 s temperature sensor Temperature compensation is available for Chlorophyll a and Rhodamine sensors only Temperature is displayed in degree Celsius Click on the Temperature Compensation box Select either Chlorophyll a or Rhodamine from the dropdown menu Data will now be corrected for temperature fluctuations Rev H Page 15 3 3 2 998 2300 C3 Submersible Fluorometer The temperature compensation coefficients for each fluorophore are listed in the table below Fluorophore Coefficient Rhodamine 0 026 Exponential Chlorophyll a 0 014 Linear Step 5 Save Calibration After calibration is complete select Save Calibration Data are now displayed as concentration estimates Raw Fluorescence Calibration Blank Subtracted Following steps 1 5 below will ensure values are reported as Blank subtracted Relative Fluorescence Units RFUB See Appendix B for recommended practices Step 1 Blanking Place the C3 Submersible Fluorometer in a blank solution Note A blank solution is a solution without the fluorophore of interest i e de ionized water artificial water or filtered water Note Ensure the optical head is free of air bubbles Wait
17. designed when fully charged to or C3 Submersible Fluorometer The exceed the Internal Data Logging capacity at battery pack has a 600m depth rating the maximum sampling rate For longer and facilitates vertical profiling and deployments at shorter sampling intervals we moored applications recommend recharging the battery after the Internal Data Logger is full or every 3 4 Charging the Battery months whichever comes first 1 Carefully align the battery s Note Use the Battery Life Calculator connector to the pins of the available on the Turner Designs website C3 charger and push the battery product page to calculate a conservative straight onto the charger estimation of how many days you can 2 Plug the charger into a standard expect to log data Factors such as battery wall outlet and allow the battery to charge and water temperature will affect charge for 6 hours for a complete logging days battery charge 3 When finished unplug the charger Specifications and remove the battery by pulling 53 D75 the battery straight out from the 13 46cm x 7 0cm charger s connector Weight in Air 1 65lbs 0 75kg Battery s Connector Capacity 2 200MAH Charger pin Connector 8 pin connector housing Protects against Protection Overcharge Over Features Discharge Overheating Short Circuiting Charge with Turner Designs charger only 998 2300 Rev H Page 34 Wiper assembly Tightening nut Appendix H Installing
18. e 1 1 or some other convenient ratio If the sample is still in the linear range the reading will decrease in direct proportion to the dilution If the reading does not decrease in direct proportion to the dilution or if the reading increases the sample is beyond the linear range Fluorometer Response Curve Sample Quenching Region Graph showing Linear and Quenching Regions of the sample s response Sample _ Linear Region Temperature Considerations Fluorescence is temperature sensitive As the temperature of the sample increases the fluorescence decreases For greatest accuracy record the sample temperature and correct the sensor output for changes in temperature For further information on how temperature light water quality and the physiological state of the algal cells can all affect the measurement of chlorophyll a please refer to the application section of Turner Designs website 998 2300 Rev H Page 28 Appendix E CFINS Installation Guide Requirements Turner Designs C3 Submersible Fluorometer with extender cable boosters required for cable lengths gt 10M ArcGIS Version 10 PC with two com RS282 serial ports or PCMCIA slot GPS with com port RS232 set to output NMEA and GPS serial cable ArcMap C3 Extension Steps Prepare C3 Fluorometer e Run C Soft and set up C3 for log sampling interval of 1 second see Log Setup Section 3 4 of C3 User s Manual e Initiate datalog session as per in
19. e Oil 0 2 ppb PTSA 0 2700 ppb PTSA 410 600 nm Refined Fuels 2 ppb NS 0 10 000 ppb NS 300 400 nm Optical Brighteners 0 6 ppb PTSA 0 15 000 ppb PTSA 445 15 nm Fluorescein Dye PTSA Dye 0 01 ppb 0 650 ppb 405 10 nm Quinine Sulfate in 0 05 Molar H2SO4 1 3 6 8 Pyrenetetrasulfonic Acid Tetrasodium Salt 1 5 Napthalene Disulfonic Disodium Salt Custom optical configurations are available on request 998 2300 Rev H Page 4 C3 Submersible Fluorometer 1 3 Important Note Regarding Use of Fluorescein Optics For C3 s manufactured prior to 2 15 2011 the Fluorescein sensor should not be enabled for simultaneous data collection with Chlorophyll and or Rhodamine sensors or it will produce unreliable results For C3 s manufactured after 2 15 2011 this interference issue was corrected If you would like to send your C3 back to Turner Designs to resolve this interference issue please contact sales turnerdesigns com 998 2300 Rev H Page 5 C3 Submersible Fluorometer 2 Inspection and Setup 2 1 Instrument Checklist The C3 Submersible Fluorometer package P N 2300 000 includes C3 Submersible Fluorometer Factory installed temperature sensor Interface cable for communicating with your PC and supplying power to the instrument CD including C Soft software user s manual and quick start guide emperature Sensor 12V Power Supply Computer Interface Cable 998 2300 Rev H Page 6 C3 Subme
20. e able to graphically view downloaded csv data files using C Soft software if desired Select files to save Total Files oO Used Data Log Start Datel Time End Date Time Records Save S 0 PRVUKP Rea epee epee epee cep eee pA eee p eat pirang Start Dated Time End Date Time Records Save 0 Download Data Clear Datalog xl 998 2300 Rev H Page 20 C3 Submersible Fluorometer 4 Maintenance and Warranty 4 1 Maintenance 4 1 1 Rinsing The C3 Submersible Fluorometer should be rinsed or soaked in freshwater following each deployment 4 1 2 Care for the bulkhead connector Install the 8 pin female end plug to the bulkhead between uses A light coat of Silicone spray should be used on the rubber of the male pins of the bulkhead to aid in sealing The manufacturer recommends 3M Silicone Lubricant Spray or Loctite 8021 spray Note You should avoid using silicone grease Do NOT use WD 40 it will destroy the connectors 4 2 Warranty Terms Turner Designs warrants the C3 and accessories to be free from defects in materials and workmanship under normal use and service for a period of 12 months from the date of shipment from Turner Designs with the following restrictions e Turner Designs is not responsible for replacing parts damaged by accident or neglect Your instrument must be installed according to instructions in the User s Manual Damage from corrosion is not covered Damage caused by customer modifica
21. e provided with the Battery Bracket to remove the installed plastic green support line from the base cap of the C3 Submersible Fluorometer 5 Slide the submersible battery pack through the bracket towards the male connector on the C3 6 Mate the 8 pin male connector on Insert green line the C3 with the female connector C3 base remove cap installed green 7 Secure the battery connection line using the Allen Hex Key provided to tighten the Hex Screw located on the bracket s locking ring 2 Pull the base cap off of the C3 around the battery Submersible Fluorometer 3 Position the Battery Bracket with the C3 so the alignment strips line up with one another and the bracket s alignment pin fits into the C3 s alignment hole Fully mated Allen Hex battery pack Screw for E locking ring Bracket locking y ring i Alignment Battery fers Bracket base 998 2300 Rev H Page 33 Appendix G Connecting the Submersible Battery Pack Introduction General Precautions The Submersible Battery Pack Under no circumstances should you attempt to P N 2200 601 contains a open the battery housing Please contact rechargeable lithium ion battery and Turner Designs if you have any problems or battery pack charger The battery questions related to the battery pack pack is used together with the Internal Datalogger to provide self contained Battery Life use for the C6 Multi Sensor Platform The battery is
22. f 0 Send File Receive File Capture Text Send Text File Capture to Printer 2 When you click on Capture Text a window will appear asking you to name and save the txt file to the location of interest 21x Folder C ADocuments and File SEA TUANERGE SIGN SS CAPTURE TAT Browse 3 Once you are finished you will need to return to the HyperTerminal window toolbar choose Transfer and then Capture Text and select Stop This will stop data capture to the txt file File Edit View Call Transfer Help oel gls a sf Send File Rertejve ap Send Text File Pause Resume Capture to Printer y 998 2300 Rev H Page 44
23. he Connect To screen will appear Choose the appropriate communications port in the Connect Using window Click OK 4 The Port Settings screen will appear Choose the following port settings Bits per second 9600 21 xi Data bits 8 Port Settings Parity None Stop bits 1 Bits per second EauN Flow control Hardware eet ata bits Click Apply and OK Parity None Stop bits fi Flow control Hardware Restore Defaults Cancel Apply 5 Next the HyperTerminal window will appear You are now ready to connect the C3 Submersible Fluorometer to a power source Data will be displayed at set intervals in the HyperTerminal window after 100 seconds or at the specified log start time C Soft HyperTerminal A F m iol x File Edit View Call Transfer Help Dle 3 3 ala l RhodaminePhycocyani Depth Temp C 50 69 129 70 0 00 23 71 Date Time 6 16 08 15 07 24 6 16 08 15 07 25 01 09 138 71 0 00 23 72 6 16 08 15 07 26 91 01 130 83 0 00 23 72 6 16 08 15 07 27 91 23 130 95 0 00 23 72 6 16 08 15 07 28 01 09 130 95 0 00 23 72 6 16 08 15 07 29 91 01 138 92 0 00 23 72 6 16 08 15 07 30 91 23 131 10 0 00 23 72 998 2300 Rev H Page 43 Most applications will integrate streaming data Below are instructions on how to save streaming data on a PC To save data 1 On the HyperTerminal window toolbar choose Transfer and then Capture Text Die File Edit View Call Transfer Help ae 2
24. ighten the arm locking screw 998 2300 Rev H Page 35 Threaded ends for easy installati removal NOTE The End Cap comes with an installed Appendix Installing the Flow Cap Introduction The Flow Cap P N 2300 700 for the C3 Submersible Fluorometer enables flow through mode The Flow Cap will allow the C3 Submersible Fluorometer to be configured with other instruments used in flow through systems The C3 Flow Cap 1 provides elimination of other light sources that may interfere with sensor measurements 2 protects sensors from damage during deployment or transport and 3 helps limit biofouling The Flow Cap can be installed on C3 Submersible Fluorometers with or without the mechanical wiper The Flow Cap can also be used as a calibration cup for C3 sensors using standard calibration solutions See following page for calibration cup use Features Flow Cap Housing on and N D O Ring that should not be removed or sealing will be compromised 998 2300 Installation Instructions 1 Turning clockwise fully hand tighten the Flow Cap housing onto the C3 Submersible Fluorometer The port located on the Flow Cap Housing should be closest to the optical head 2 Turning clockwise hand tighten the End Cap onto the Flow Cap Housing until it is fully tightened 3 Flow Cap installation is complete OUT FLOW PORT D NOTE For optimal 5 IN FLOW use Turner Designs O PORT recom
25. ion to a amp Open Connection Opens a GPS connection for updates from GPS amp Close Connection Closes the GPS connection to stop updates from GPS Ronnecioa toa Start Streaming To Log Starts streaming GPS data into the selected log connecting u Stop Streaming To Log Stops streaming GPS data into the selected log d with ArcMap i Stamp Current Position To Log Stamps the current position to the log ping data streaming li Pan To GPS Position Pans the active view to display the current GPS position Zoom To GPS Position Zooms to the current GPS position P Add Destination Sets the clicked point on the screen as the destination GPS toolbar tools and functions Steps 1 Click Customize on the Main menu point to Toolbars and click GPS The toolbar is added Q Tip To hide the GPS toolbar right click any toolbar and click GPS Related Topics About connecting to a GPS device Getting started with ArcMap GPS Support e Set up C3 Connection using C3 Toolbar Setup C3 e Choose Com Port and Channel Settings then hit connect button Data should start streaming in as shown below C3 Com Port coma C4 Status Pon Open C3 Data Walid Enable Mame Units Value Chan 1 M Rhodamine PPE e 20 Chan 2 I Turbidity NTU 11 92 Chan 3 fe Chlorophyll a a7 7 16 Lx Date Time Depth m Temp C fiv220 10 06 55 4 66 19 12 llf22 10 10 06 52 8 36 10 84 7 380 9 66 19 12 llf22 10 10 06
26. ions C FINS Installation Guide Installing the Battery Bracket Connecting the Submersible Battery Pack Installing the Mechanical Wiper Installing the Flow Cap Using the Secondary Solid Standard C Series Analog Adapter How to set up Hyperterminal for Digital ASCII Data Capture TAS TTOQOMIMVOWD D gt off A O NO C3 Submersible Fluorometer Equipment Specified as Electrical and Electronic Waste C3 Submersible Fluorometer 1 Introduction 1 1 The C3 Submersible Fluorometer is manufactured according to users optical specifications with one two or three optical sensors ranging in wavelengths from deep ultraviolet to infrared Each optical sensor is designed with fixed excitation and emission filters The C3 Submersible Fluorometer comes with a factory installed temperature probe and can be configured with a pressure sensor for depth measurements up to 600 meters and or a wiper to minimize biofouling The C3 Submersible Fluorometer Windows based user interface allows for easy calibration of each sensor digital data reporting data logging and digital or analog export of data 1 2 Optical configurations for Turner Designs standard applications ER Minimum Application Detection Limit Dynamic Range Excitation Emission In Vivo Chlorophyll a 0 025 ug L 0 500 ug L 685 50 nm Phycoerythrin Marine Cyanobacteria 150 cells ml 0 150 000 cells ml 575 10 nm Si boM cbom fo 4 ppb QS 0 2500 ppb QS 430 30 nm Crud
27. lanked RFUB calibration if you do not have a standard with Known concentration or if you are unsure what numeric value to assign your standard NOTE Repeat either one of the above calibrations for each channel that will be used for Analog Output 998 2300 Rev H Page 40 Once calibration is complete click the Log Setup tab to set the logging start time amp logging interval Click enable datalog wait for C Soft to automatically shutdown and disconnect power As soon as power is re supplied to the C3 via the C Series Analog Adapter it will automatically begin data output after 90 seconds or pre configured start time at the set interval Analog Output The C Series Analog Adapter converts digital data to voltage to help users integrate our in situ fluorometers with almost any CTD third party system or data logger that accepts a 0 5 volt signal Below are two ways to interpret voltage output based on the C3 s setup Reading the Voltage Output for RFUB Calibrated C3 s The voltage output from the C3 Submersible Fluorometer via the C Series Analog Adapter needs to be blank corrected Users can standardize the voltage signal using a standard of known concentration to convert RFUB to direct concentrations Reading the Voltage Output for Specific Range Calibrated C3 s The voltage output from the C3 Submersible Fluorometer via the C Series Analog Adapter needs to be blank corrected The voltage output reflects direct concentratio
28. led to a standard they are relative values 998 2300 Rev H Page 14 C3 Submersible Fluorometer 3 3 Calibration Procedure 3 3 1 998 2300 Direct Concentration Calibration Following steps 1 5 below will ensure values are reported as blank subtracted Concentrations in units of choice See Appendix B for recommended practices Step 1 Blanking e Place the C3 Submersible Fluorometer in a blank solution and ensure the optical head is free from air bubbles Note A blank solution is a solution without the fluorophore of interest i e de ionized water artificial water or filtered water Wait until Current RFU readings have stabilized then select the Set Blank button to set the blank value Wait for the blank value to be measured and set for all 3 gains Step 2 Standard Value and Units Place the C3 Submersible Fluorometer in a standard solution or use a secondary solid standard see Appendix D for more information on solid standards Note UV and Turbidity sensors cannot be used with solid standards Enter the known concentration value of the standard solution or solid standard in the Standard Value box If the concentration is not Known enter an arbitrary value Enter manually or select desired units from the dropdown menu Note Do not choose or enter RFUB if Direct Concentration Mode is preferred Step 3 Set Standard When readings in Current RFU box have stabilize
29. lti parameter systems such as CTD s and 3 Digital data that can be read and converted to analog data by the C Series Analog Adapter An 8 pin Impulse cable provides two end connections 1 a 9 pin RS282 serial port for connection to a PC or laptop computer and 2 a 12V port for supplying power to the unit C3 Submersible Fluorometer bulkhead and serial port connectors OGOGO O Sub D Connector Wire Guide Brown DIR amp DSR PIN 4 6 7 Yellow RTS amp CTS PIN 7 8 8 Orange N A N A Power ground and V Batt are not common 998 2300 Rev H Page 26 C3 Interface Cable P N 2200 150 This cable is included as a standard accessory with the C3 It is used to i program and calibrate the C3 and provide real time data view The cable length is approximately 2 5 Computer Interface Cable Computer Integration Cable C3 Integration Cable P N 2200 160 and 2200 165 This cable is identified by the yellow band of heat shrink The integration cable is used to connect the C3 to a PC or data logger for continuous data capture It prevents interruptions in the C3 data transmission to the PC yellow heat shrink The cabled configuration allows for real time data transmission The C3 measurements are sent from the RS 232 cable to a PC where the fluorescence data can be viewed and recorded on a computer or a data logger that can record digital ASCII data The data can also be saved on MS E
30. mends positioning the C3 Pro unit vertically when a the Flow Cap is installed This will purge any air from the system that might cause skewed readings Rev H Page 36 Calibration Cup Use With the C3 Submersible Fluorometer s Flow Cap Housing installed 1 Remove the End Cap 2 Use a port plug to plug the port located on the Flow Cap Housing 3 Position the unit vertically with optical head facing upward see diagram to the right In this position the Flow Cap Housing can be used to hold standard solutions for calibrating the C3 sensors Specifications Max Pressure 100 psi Length 10 1 cm 3 98 in Diameter 10 cm 3 94 in Weight 455 g 1 003 lbs Max Volume 400 ml 998 2300 Rev H Page 37 Port Plug Appendix J Introduction The Solid Standard P N 2300 900 for the C3 Submersible Fluorometer provides a very stable fluorescent signal which can be adjusted to e Match a specific sample e Create a calibration correlation Using the Secondary Solid Standard Installation and Use 1 To establish a correlation between a known concentration and its respective fluorescence output immerse the sensor in a sample with a known concentration of your fluorophore 2 Dry off the C3 Submersible Once a correlation is established Fluorometer s optics attach the between the Solid Standard and a Solid Standard Cap liquid primary standard or typical s
31. ns re scaled to 0 5 volts Actual Concentration blank subtracted voltage 5 volts x concentration at 5 volts Example if your measured voltage for a given sample is 2 567 volts and the blank voltage is 0 067 volts your blank subtracted voltage is 2 5 volts 2 5 volts 5 volts 0 5 If your range maximum equals 100 ug L then 0 5 100 ug L 50 ug L Therefore a sample that measures 2 567 volts 50 ug L 998 2300 Rev H Page 41 Wiring Guide The wiring guide below indicates the function of each pin for the Analog Adapter s 6 pin male end To avoid damaging the Analog Adapter please ensure the pins are only used for their intended purpose in wre couor 1 Red VBatt Positive VBatt Negative Analog Out 1 0 5 Analog Adapter s 6 pin male end oH OF l _ oF i wy maya 6 CONTACT 5 Blue Analog Out 3 0 5 VDC 6 Brown Analog GND 998 2300 Rev H Page 42 Appendix L How to set up Hyperterminal for Digital ASCII Data Capture 1 Ensure C Soft program is not running in the background On an MS Windows computer open the HyperTerminal program Start All Programs lt Accessories Communications HyperTerminal 2 The Connection Description screen will appear Name the connection description This file will save the communication parameters for the C3 Submersible Fluorometer and can be used in the future to establish communication with the instrument quickly 3 T
32. on of how many days you can expect to log data Factors such as battery charge and water temperature will affect logging days A few seconds after the Yes button has been clicked to enable the Data Output Ready State the C Soft program will automatically shut down and users must disconnect power and serial connections The instrument is now in the Data Output Ready State and will remain in that state so long as C Soft is not used to communicate with the instrument Note As soon as C Soft is used to communicate with the instrument users will need to re set the parameter settings in the Log Setup section 3 4 and again enable datalog to put the instrument into the Data Output Ready State When power is supplied to the instrument while it is in the Data Output Ready State it will begin streaming data after a 100 second delay If power is cycled i e disconnected and reconnected please allow 100 seconds for the data stream to begin 3 6 Downloading and File Management Users can download new and existing files saved in the C3 Submersible Fluorometer memory Select Download Data Note This step may take up to 10 minutes depending on file size When download has completed choose the files to be saved by clicking the Save box Users will be prompted to choose a folder location where data will be saved Files will be saved in csv format Select Clear Datalog to erase existing data from memory Users ar
33. rsible Fluorometer 2 2 Optional Accessories gt Pressure sensor P N 2300 360 Factory installed Note Can only be installed during the manufacturing of the unit C3 Mechanical Wiper P N 2300 450 Factory installed Note Can only be installed during the manufacturing of the unit Replacement Wiper Brushes P N 2300 460 Replacement Wiper Spacers P N 2300 462 Rechargeable Submersible Battery Pack including charger P N 2200 601 Battery Bracket P N 2300 603 Note Required for use with Battery Pack and Analog Output Adaptor Flow Cap P N 2300 700 Shade Cap P N 2300 500 or C ray Shade Cap P N 2300 502 Shade Cap Weight P N 2300 510 C ray Towed Deployment Body P N 2300 750 Extender Cables 10 meters P N 105 2595 25 meters P N 105 2596 50 meters P N 105 2597 Custom lengths available upon request Booster Kit P N 2200 900 Note Required for cable lengths greater than 10 meters 24 Pigtail Interface Cable with Locking Sleeve 2200 170 24 Integration Cable P N 2200 160 or 5m Integration Cable P N 2200 165 Note Used for continuous data output and communication with the C3 NOT intended for uploading firmware to the C3 Secondary solid standard P N 2300 900 for multiple sensors Note Not available for turbidity or ultraviolet sensors C Series Analog Output Adapter P N 2300 480 Submersible Battery Mechanical Wiper
34. structions in Section 3 4 of manual C Soft will automatically shut down e Power down and power up C3 it will take 90 seconds for data to start streaming Prepare ArcMap e If not already installed install C3 extension in ArcMap double click TD_C3 esriAddIn e Select both GPS Toolbar and Turner Designs C3 toolbar using Customize Toolbars 998 2300 Customize Reopracessing GPS Toolbar C3 Toolbar Style Manager Windows Toolbars b Extensions 4dd In Manager VBA Macros Customize Mode Archlap Options gt x ors SD Analyst Advanced Editing Animation ArcScan COGO Data Driven Pages Data Frame Tools Distributed Geodatabase Draw Edit vertices Editor Effects Feature Cache Feature Construction Geocoding Tools Topology Tracking Analyst F Turner Designs C3 Utility Network Analyst Versioning Customize Rev H Page 29 e Connect to GPS on GPS toolbar See ArcMap help for information on this lt lt lt aie amp A ai Hide Locate Back Home Options Resource Center Contents Favorites Search Adding the GPS toolbar DAN x 5 ype a question then click Ask ArcGIS 10 GPS toolbar Before working with GPS devices within ArcMap you must first add the GPS toolbar sy A fa Da SERT found GPS toolbar buttons and their functions GPS toolbar Button Name o Function data concepts connect
35. t Yes Note If data logging is set to start for some past time there will be a minimum delay of 100 seconds before startup after power has been supplied Otherwise wait until the data logging start time has been reached for data logging to begin WARNING As soon as data logging is enabled users will be unable to access C Soft settings This should be the last step before the C3 Submersible Fluorometer is deployed 3 5 Enabling Data Output Ready State The Data Output Ready State must be enabled for the C3 Submersible Fluorometer in order to log data capture real time data output and or integrate the C3 with other instrumentation There are five necessary steps to ensure the instrument is properly set up for data output 1 Set the instrument s clock to PC or local time in the Settings Tab Select the sensors you expect to use during sampling Calibrate selected sensors for either Direct Concentration Mode or Raw Fluorescence Units Blanked RFUB Mode see section 3 3 4 Set the start time sampling and wiping intervals in the Log Setup Tab Note wiping interval is optional and depends on the sampling interval see section 3 4 5 Click the Enable Datalog button followed by the Yes button to enable the Data Output Ready State 998 2300 Rev H Page 19 C3 Submersible Fluorometer Note Use the Battery Life Calculator available on the Turner Designs website C3 product page to calculate a conservative estimati
36. t C Soft Chan2_Val 11 12 Channel 2 value Chan2 Unit NTU Channel 2 Units from C3 Setup Form Not C Soft Chan2_Type Turbidity Channel 2 Name from C3 Setup Form Not C Soft Chan3_ Val Channel 3 value Chan3_ Unit ug l Channel 3 Units from C3 Setup Form Not C Soft Chan3_Type Chlorophyll a Channel 3 Name from C3 Setup Form Not C Soft Lat_DD 41 22914167 Latitude in Decimal degrees Southern Latitudes negative Long _DD 173 081 7033 Longitude in Degrees Western Longitudes negative Speed over ground in GPS Display Options Speed Units UTC Date GPS date in UTC UTC Time GPS Time in UTC Flag Flag value 0 no flag 1 Flagged Channel names and units are logged as per the C3 setup form and not C soft These can be changed during a logging session but it is not recommended 998 2300 Rev H Page 32 Appendix F Installing the Battery Bracket Introduction The Battery Bracket P N 2300 603 4 Secure the connection by feeding for the C3 Submersible Fluorometer the Green Line through the holes was designed to provide support on the Battery Bracket s base allowing a direct connection between the battery and the C3 during self Insert green line rovided to contained deployment thus ae the eliminating the need for a cable connection connection The Battery Bracket also provides balance and stability for the C3 Submersible Fluorometer and battery Installation 1 Use the extra plastic green support lin
37. tion of the instrument is not covered e This warranty covers only Turner Designs products and is not extended to equipment used with our products We are not responsible for accidental or consequential damages except in those states where this limitation is not allowed This warranty gives you specific legal rights and you may have other rights which vary from state to state e Damage incurred in shipping is not covered Extended warranties are available for purchase Contact sales TurnerDesigns com 998 2300 Rev H Page 21 C3 Submersible Fluorometer 4 3 Warranty Service To obtain service during the warranty period the owner shall take the following steps 1 Write email or call the Turner Designs Technical Support department and describe as precisely as possible the nature of the problem Phone 1 877 316 8049 Email support turnerdesigns com 2 Carry out any adjustments or tests as suggested by the Technical Support Department 3 If proper performance is not obtained you will be issued a Return Materials Authorization number RMA to reference Package the unit write the RMA number on the outside of the shipping carton and ship the instrument prepaid to Turner Designs If the failure is covered under the warranty terms the instrument will be repaired and returned free of charge for all customers in the contiguous continental United States For customers outside of the contiguous continental United States who p
38. until Current RFU readings have stabilized then select the Set Blank button to set the blank value Wait for the blank value to be measured and set for all 3 gains Step 2 Selecting Units Select RFUB from the units dropdown menu Note Do not enter a value in the standard value box Step 3 Skip and go to Step 4 Step 4 Optional Temperature Compensation Follow same steps as the Direct Concentration Calibration procedure in Section 3 3 1 Step 5 Save Calibration After calibration is complete select Save Calibration Data are now displayed as blank corrected relative fluorescence units Rev H Page 16 3 3 3 3 3 4 998 2300 C3 Submersible Fluorometer Raw Fluorescence Calibration In the Current Data screen select the calibrate button next to a specific channel At the bottom of the Calibration Screen select click to use Uncalibrated Raw Fluorescence Mode Data for that channel will be uncalibrated and reported as Relative Fluorescence Units RFU Depth Calibration Optional Accessory Depth is displayed in meters only There are two calibration options for depth 1 The first uses only the atmospheric pressure as an offset This is suitable for any shallow application including most fresh water applications 2 The second also compensates for gravity variations with latitude as designated from the UNESCO Technical Papers in Marine Science 44 This method
39. urchased equipment from one of our authorized distributors contact the distributor If you purchased directly contact us We will repair the instrument at no charge Customer pays for shipping duties and documentation to Turner Designs Turner Designs pays for return shipment custom duties taxes and fees are the responsibility of the customer 4 4 Out of Warranty Service Follow steps for Warranty Service as listed above If our Technical Support department can assist you by phone or correspondence we will be glad to at no charge Repair service will be billed on a fixed price basis plus any applicable duties and or taxes Shipment to Turner Designs should be prepaid Your bill will include return shipment freight charges Address for Shipment Turner Designs Inc 845 W Maude Ave Sunnyvale CA 94085 998 2300 Rev H Page 22 C3 Submersible Fluorometer Equipment Specified as Electrical and Electronic Waste 998 2300 Rev H Page 23 Appendix A C3 Submersible Fluorometer Specifications Signal Output Digital ASCII or Analog Note analog requires accessory Nonvolatile Memory 64 000 Data Lines Power Draw Max 1 Watt Submersible Battery Pack Battery Lithium lon Voltage Output 14 8V nominal Protects against overcharge over discharge overheating Protection Features ae short circuiting 998 2300 Rev H Page 24 Appendix B C3 Submersible Fluorometer Recommended Practices Identification sensors
40. users to set the time and date identify each optical sensor update serial numbers and enable disable sensors 998 2300 Users can manually set the date and time or choose to synchronize the C3 Submersible Fluorometer to the PC date and time Each channel 1 3 in the Settings Screen has a location associated with it on the C3 Submersible Fluorometer that is numerically labeled 1 3 respectively Channels 4 6 are designated for the C6 Multi Sensor Platform and are disabled for the C3 Click on the box adjacent to the channel to enable channel operation If there is no sensor installed users will be unable to select that channel Channels can be renamed using the dropdown selections or by manually entering a name The serial number boxes are designed for the C6 Multi Sensor Platform There is no serial number associated with the sensors in the C3 Settings will automatically be saved after exiting the Settings screen Rev H Page 11 C3 Submersible Fluorometer File Settings COMPort Window Channel Settings Chan 2 Chan3 iv Phycocyanin Chan 6 Depth Temp Enable Name Serial Chani M Rhodamine Connected Settings Current Data Log Setup Download Data C Soft Manager E xj E Unit Current Time 10 32 20 Retrieve Unit Time 6 16 2008 E Chlorophyll a El B Current Time 10 32 27 Set Unit To PC Date amp Time 6 16 2008 E B v Manually Set Unit Date amp
41. xcel from your C Soft Software In order to provide greater length extender cables P N s 105 2595 10 M 105 2596 25M or 105 2597 50 M and custom cables up to 100M can be installed along with the boosters P N 2200 900 here between the C3 and the interface cable to allow the signal and power to travel greater distances This is the 12VDC power supply P N 159 0191 It is included as a standard accessory with the Cs The power supply connects to the 12 VDC connector on the interface or integration cable which will supply power to the C3 This is where DC power is connected This is the RS 232 connection to the PC 998 2300 Rev H Page 27 Appendix D Linear Range Quenching and Temperature Considerations The linear range is the concentration range in which the C3 sensor output Is directly proportional to the concentration of the signal The linear range begins with the smallest detectable concentration and spans to an upper limit concentration that is dependent upon the properties of the material filters used and path length A non linear relationship is seen at very high concentrations where the signal does not increase at a constant rate in comparison to the change in concentration see figure below At even higher concentrations the signal will decrease even though the sample concentrations are continuing to increase This effect is known as signal quenching Linearity can be checked by diluting a sampl

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