Jenway 6850 Operating Manual
Contents
1. WL 448 8nm Abs 13 23 7 9 1 21 Multi Wavelength Test 5 WL D 2 m gt u Maz 10 4 STORING METHODS RESULTS Insert a cuvette containing the sample to be analysed into the sample chamber and press the key below the sample measurement icon The instrument will take a reading at each of the specified wavelengths the operating menu screen will then display the result of the selected measurement calculation and the photometric readings for each of the measured wavelengths A method result file can be saved to the instrument s internal memory In the multi wavelength mode screen press the Save key on the instrument keypad The instrument will ask the user to enter a filename max 8 characters using the alpha numeric keypad The user can select the indicated letters numbers and symbols by repeatedly pressing the key until the required value is displayed after a short period the next character can be entered in the same manner Once the filename has been entered press the Enter key to confirm 10 5 RECALLING STORED RESULTS WL 448 0nm Abs 13 23 0 Search scroll Open file ULTI mul A method result file can be opened from the instrument s internal memory In the multi wavelength mode screen press the Open key on the instrument keypad The user can select from the list of stored files by pressing the up and down arrow keys Once the required file has been located press the Enter key to confirm I2. 32 SECTION 7 SPECTRUM The spectrum measurement mode enables measurements of absorbance or transmittance over a range of wavelengths to be performed The absorbance or transmittance at each wavelength is plotted graphically Post measurement tools such as peaks and spectral points analysis can be performed This operating mode can be used to partially characterise a sample 7 1 SPECTRUM MODE SCREEN 440 tinm Abs i my Scale EY 190 0 Wavelength nm Ld 7 2 METHOD SETUP 448 Unm Abs The spectrum measurement mode enables measurement parameters to be changed and post measurement tools to be accessed The four options at the bottom of the screen allow the user to edit the scan settings select the measurement mode perform a post measurement search of the spectrum or apply a smoothing algorithm to the displayed scan These options are selected with the corresponding function buttons The spectrum scan settings can be edited by pressing the function key below the Setup option The options that can be changed are 1 Scan from Highest Wavelength 2 Scan to Lowest Wavelength 3 Scan Interval 4 Scan Speed The numeric keypad should be used to enter the required start and end wavelengths and the up and down arrow keys are used to select from the available scan interval and scan speed options Press the Enter key to confirm 33 7 3 SELECTING THE MEASUREMENT MODE The measurement mode ca
3. 9 4 ADJUSTING THE CONCENTRATION CALCULATION FACTORS NN 42 9 5 SELECTING THE UNIT OF MEASUREMENT cuca ee ee 42 9 6 RESET MODE SETTINGS ES ita 43 9 7 DNAPROTEIN MEASUREMENT S su iin 43 9 8 SIORING METHOBSIRESULTS sn nn este dunes 43 9 9 RECALLING STORED RESULTS SE Ne desde de rene ue 44 SECTION 10 MULTI WAVELENGTH hier 45 10 1 MULTEWAVELENGTH MODE OPTIONS zn item eot 45 10 2 WAVELENGTH SELECTION santa ei 45 103 SAMPLE MEASUREMENT essen een inde ee 46 104 STORING METHODS RESULT S zn se en 46 105 RECALLING STORED BESUL TS eisen 46 SECTION 11 SAVING RECALLING RESULTS USING A USB MEMORY STICK47 SECTION 12 ACCESSORIES AND SPARE PARTS eese 48 11 1 OPTIONAL ACCESSORIES rire se sen ca Iona tapa pea ca ah 48 11 2 INSTALLING THE PASSIVE AND ACTIVE ACCESSORIES essen 48 11 2 1 Passive Accessories ccccccssececceeseeeccesseccceuseeecseseecseuseeessaeeecsaseesseasecessageeesseneeesseasensess 48 11 2 2 ACCOSSOFLIOS tun a ea lee A 49 11 2 2 1 8 Position Automatic Cell Changer iii 49 11 3 USING THE 8 POSITON AUTOMATIC CELL CHANGER eene 50 nn e rn a e a a 51 SECTION 12 MAINTENANCE AND SETVICE occoccccccccccncncccnncncccncncnccacocncncnanonos 52 12 1 ROUTINE MAINTENANCE ana ee 52 122 CAMP REPLACEMENT ea Rd ein sus de neue 52 12 2 1 Tungsten Lamp hHeplacemiell uii per aee o rn ases ue dtd ect d ata we et ed ad er 52 12 2 2 Deuterium Lamp Rep
4. Time s AA eee E Press ESC to quit search mode 39 8 8 STORING RESULTS Time s Please input File Name KINETICS_ 8 9 40 RECALLING STORED RESULTS WL 688 Gnm Abs e Time s DIT BA INETICH kin A result file can be saved to the instrument s internal memory Press the Save key on the instrument keypad The instrument will ask the user to enter a filename max 8 characters using the alpha numeric keypad The user can select the indicated letters numbers and symbols by repeatedly pressing the key until the required value is displayed after a short period the next character can be entered in the same manner Once the filename has been entered press the Enter key to confirm A result file can be opened from the instrument s internal memory Press the Open key on the instrument keypad The user can select from the list of stored files by pressing the up and down arrow keys Once the required file has been located press the Enter key to confirm SECTION 9 DNA PROTEIN The DNA Protein measurement mode allows the user to measure multi wavelength absorbance ratios such as 260nm 280nm and 260nm 230nm which are commonly used to estimate a protein or nucleic acid sample s purity The mode also includes calculations that can be used to estimate the concentration of the protein or nucleic acid sample 9 1 DNA MENU OPTIONS 130 Anm Abs DNA Protein Test The DNA Protein
5. power switch on the rear of the instrument The instrument will initially perform several power on tests before displaying the warm up screen WL 260 G nm I 13 58 09 JENWAY 6850 L Wait until 05 booted Warm up 15 ninmutes Fig 2 2 Instrument power on tests complete The power on tests comprise 1 Lamp test 2 Detector test 3 Ato D converter test 4 Grating position check 5 Dark current check 2 3 DISPLAY The instrument has an inclusive display which enables text and graphs to be displayed clearly Following successful completion of the power on tests and the instrument warm up time the main menu screen will be displayed WI 440 Unn TT JENWAY 6850 LRO Juantitation Node scan tics Mode Fig 2 3 Display Main menu options 1 Photometrics measurement mode DNA Protein measurement mode Quantitation measurement mode Multi wavelength measurement mode Instrument settings menu Slit width DN S Y 2 3 Spectrum measurement mode 4 Kinetics measurement mode 10 2 4 CONTROLS The keypad used for this model enables an easy and effective way of navigating the different measurement modes entering numbers saving and analysing results The keypad is displayed below START STOP Fig 2 4 Keypad Function Keys Allows on screen options to be selected Co Swxvz Numeric Keys Enter numbers and letters Select corresponding menu options CLEAR CLEAR Key
6. Fax 44 0 1785 810405 55 SECTION 14 DECLARATION OF CONFORMITY Declaration of Conformity ER TRITT AR A A PR RS RE LE UV Visible Spectrophotometer Model 6850 This product complies with the requirements of the EU Directives listed below 2004 108 EC EMC Directive 2006 95 EC Low voltage Directive LVD Compliance with the requirements of these Directives is claimed by meeting the following standards EN 61326 1 2006 Electrical Equipment for Measurement Control and Laboratory use EN 61010 1 2001 Safety Requirements Electrical Equipment for Measurement Control and Laboratory use CE mark affixed 2012 Ld r r af av a Signed e an rn Mr S Marriott Date 4 2 EZ Authority Technical Director Bibby Scientific Ltd Bibbv Scientific Bibby Scientific Ltd Stone Staffs ST15 OSA UK Tel 44 0 1785 812121 Fax 44 0 1785 813748 56
7. left or right arrow key to enable a search option The number of the result that is to be viewed is entered using the numeric keypad Press the Enter Key to confirm 44 SECTION 10 MULTI WAVELENGTH The multi wavelength measurement mode allows the user to measure a sample s photometric absorbance or transmittance at up to 10 wavelengths Following sample measurement the photometric readings are displayed on the multi wavelength mode screen 10 1 MULTI WAVELENGTH MODE OPTIONS WL 198 Gnm Abs 13 10 34 Multi Wavelength Test 1 WL D2 zum Y WL nm DM E 10 2 WAVELENGTH SELECTION WL 198 8nm Abs Setup Wavelength B5elect The multi wavelength measurement mode enables measurement parameters to be changed The two options at the bottom of the screen allow the user to edit the measurement wavelengths and change the measurement mode These options are selected with the corresponding function buttons The measurement wavelengths of the selected measurement mode can be adjusted by pressing the function key below the WL Setup option or the GOTOA key on the instrument keypad The required wavelengths can be entered using the numerical keypad pressing the Enter key to confirm each value Further values can be added by moving to a new line with the down arrow key Once all the required wavelengths have been entered press the ESC key to exit the wavelength selection screen 45 10 3 SAMPLE MEASUREMENT
8. nm Abs Setup Standard Conc the function key below the Standard option The user will be prompted to enter the concentration values for each standard The values can be entered using the numerical keypad Press the Enter key to confirm To add additional standard values to the table press the down arrow on the keypad Once all values have been entered press the Esc key on the keypad to return to the calibration table settings screen 6 3 2 4 Display Calibration Curve Completed calibration curves can be viewed graphically by pressing the function key below the Show Curve option 29 6 4 CREATING A NEW STANDARD CURVE The user must firstly edit the calibration table before attempting to create a new standard curve 30 148 Gnm Abs Insert the cuvette containing the blank solution into the reference position in the sample chamber and insert the cuvette containing the first standard solution into the sample position Close the instrument lid and press the start stop button Once the measurement is complete the instrument will ask for the second standard solution to be inserted Repeat the measurement procedure for each standard solution Once all standard solutions have been measured the new standard curve and curve equation will be shown The standard curve can be viewed graphically by pressing the function key below the show curve option on the calibration table screen 6 5 STORING A STANDARD CU
9. produce the colour to be measured Deviations from the Beer Lambert Law may occur at high and low concentrations giving non linear response during sample concentration measurements For all new methods a linear range should be defined by the preparation of a calibration curve The quantitation mode may be used to construct such a curve against which sample results are automatically measured Cuvettes and sample holders must be filled to a minimum level which covers the light path All Jenway spectrophotometers have a beam height of 15mm The instrument must be calibrated to zero absorbance 100 transmittance prior to taking readings In the spectrum measurement mode a baseline scan must be performed before performing a sample scan 17 SECTION 4 INSTRUMENT SETUP 4 1 INSTRUMENT NAVIGATION To navigate around the spectrophotometer screen press the arrow keys on the main keypad and use the Enter key to select the highlighted option Alternatively some options and actions require the user to press the function and numeric keys on the instrument keypad The Esc key can be used to return to the previous menu without saving any changes 4 2 MAIN MENU SCREEN The main menu screen provides access to all WL 448 Unm TY measurement modes the system utility menu EX 16850 and the variable slit width setting The available A measurement modes are photometrics 2 Qua itation Mode RE TE quantitation spectrum scan ki
10. standard or factor Display Graphical and concentration Analysis Slope and line of best fit between any two points Range Any range between 190 to 1100nm Scan speed 100 to 2000nm min Scan interval Selectable 0 1 0 2 0 5 1 2 or 5nm DNA DNA RNA Ratio concentration A320 correction modes Measurement Direct UV SECTION 2 Installation 2 1 UNPACKING Remove the 6850 from the packaging and ensure the following items are included 1 Model 6850 spectrophotometer fitted with 10 x 10mm cuvette holder 685 SC 2 Power supply cables HH179 S UK lead HH180 S EU lead CABLEUS US lead 3 Instruction manual 685 030 4 10mm Glass Cuvettes x 4 035 027 5 10mm Quartz Cuvettes x 2 035 028 6 Dust Cover 685 040 7 PC software CD and USB security dongle 685 035 2 2 INSTALLATION The 6850 is supplied ready to use The working temperature and humidity range of the 6850 spectrophotometer is 15 35 C 15 70 relative humidity The storage temperature and humidity range of the 6850 spectrophotometer is 10 50 C 15 70 relative humidity The unit should be placed on a clean flat surface which is free from drafts and vibrations The units are designed for operation on 110V 11V 60Hz 1Hz and 220V 22V 50Hz 1Hz AC Connect the power supply unit to the power inlet socket on the rear panel of the instrument and connect to the mains socket Turn the power on at the mains and switch the instrument on using the
11. 00 Abs measurement parameters to be changed The four options at the bottom of the screen allow the user to select the unit of measurement the measurement mode the concentration factor and the calibration standard s concentration These options are selected with the corresponding function buttons Mode Factor 5 2 METHOD SET UP The photometrics measurement mode is very simple and the only parameters which can be adjusted are the wavelength the units of the measurement the measurement mode and the concentration setup parameters 5 2 1 Selecting a Wavelength The wavelength can be adjusted by pressing the GOTOA key on the instrument keypad The required wavelength can then be entered using the numerical keypad Press the Enter key to confirm 5 2 2 Selecting the Measurement Mode The measurement mode can be selected by pressing the function key below the Mode option The required measurement mode can then be selected from the available options of Abs T and Conc Factor using the up and down arrow keys Press the Enter key to confirm 23 5 2 3 Selecting the Unit of Measurement The unit of measurement can be selected by pressing the function key below the Unit option The required unit of measurement can then be selected from the available options of IU mM L ML ug mL mg mL mg L mEq ppb ppm and other Press the Enter key to confirm 5 2 4 Entering a Concentration Factor The concentration of a sample can be d
12. 88 Gnm Abs 12 15 10 0 Run Time 30 The kinetics scan settings can be edited by pressing the function key below the Setup option The options that can be changed are 1 Run Time 2 Delay Time 3 Time Interval The numeric keypad should be used to enter the required run and delay times and the up and down arrow keys are used to select from the available time interval options Press the Enter key to confirm 37 8 3 1 Selecting the Measurement Mode The measurement mode can be selected by pressing the function key below the Mode option The required measurement mode can then be selected from the available options of Abs and T using the up and down arrow keys Press the Enter key to confirm 8 4 8 5 38 SAMPLE MEASUREMENTS 6880 8nm Abs Time s Setup Mode Process Search Scale scale Time s 30 Node Process search Insert a cuvette containing the blank solution into the reference position in the sample chamber and insert the cuvette containing the sample solution into the sample position Close the instrument lid and press the Start Stop key on the keypad Once the measurement is complete the measured kinetics scan will be displayed on the screen ADJUSTING THE DISPLAYED SCAN RANGE The instrument will display the full range scan after a measurement is complete To change the displayed time range press the left or right arrow keys The start and end time
13. Delete the entered value or stored data BACK Key Delete a single character 11 12 ESC 100 T OABS SAVE PRINT ENTER CELL G co 2 35 O Om gt US Yv 4 ESC Key Return to previous menu screen 100 T 0Abs Key Zero Blank instrument OPEN Key Open results stored in internal memory SAVE Key Save results to internal memory START STOP Key Start Stop measurement GOTO Key Set the instrument wavelength using the numeric keys Press the Enter key to confirm PRINT Key Print result ENTER Key Confirm operation CELL Key Select Deselect Auto cell Holder position If fitted Use the numeric keys 1 8 to move to cell holder to the corresponding cell position RIGHT LEFT Keys Search peak valley and set X axis scale UP DOWN Keys Scroll menu data and set Y axis scale 2 5 REAR PANEL The image below shows the rear panel of the instrument 2 6 oF D e m WN 2 Fan Cover Power switch Power in socket LCD contrast adjustment Parallel port USB port FRONT VIEW Fig 2 5 Rear Panel Allows access to lamp when replacement is necessary On off switch for the unit Connection socket for power supply unit Connection to a PC or external serial printer Allows the accessory printer to be connected Allows the instrument to be connected to a PC The image below shows the front view of the instrument B NP a LCD display Keypad Sample chambe
14. JENWAY Operating Manual Bibby Scientific 685 030 REV B 06 14 Safety Please read this information carefully prior to installing or using this equipment de The unit described in this manual is designed to be operated only by trained personnel Any adjustments maintenance and repair must be carried out as defined in this manual by a person qualified to be aware of the hazards involved It is essential that both operating and service personnel employ a safe system of work in addition to the detailed instructions specified in this manual Other than for those items defined in the maintenance procedures herein there are no user serviceable items in this instrument Removal of covers and attempted adjustment or service by unqualified personnel will invalidate the warranty and may incur additional charges for repair References should always be made to the Health and Safety data supplied with any chemicals used Generally accepted laboratory procedures for safe handling of chemicals should be employed If it is suspected that safety protection has been impaired in any way the unit must be made inoperative and secured against any intended operation The fault condition should immediately be reported to the appropriate servicing authority Merci de lire attentivement ces informations avant d installer ou d utiliser cet appareil 1 L appareil d crit dans ce manuel est con u pour tre utilis uniquement par des pers
15. No and press the Enter key to confirm Selecting the Yes option will result in all the absorbance value measured at wavelength 3 being subtracted from the photometric values measured at wavelengths 1 and 2 9 3 WAVELENGTH SELECTION The measurement wavelengths of the selected measurement mode can be adjusted by pressing the GOTOA key on the instrument keypad The required wavelengths can then be entered using the numerical keypad Press the Enter key to confirm 9 4 ADJUSTING THE CONCENTRATION CALCULATION FACTORS ET i T The measurement mode option can be selected by m UL 338 8nm Abs 14 08 DNA Protein Test pressing the function key below the Coeff option EL AN Y I DE The new concentration calculation factors can be entered using the numeric keypad Press the Enter key to confirm 9 5 SELECTING THE UNIT OF MEASUREMENT The unit of measurement can be selected by pressing the function key below the Unit option The required unit of measurement can then be selected from the available options of IU mM L M L ug mL mg mL mg L mEq ppb ppm and other Press the Enter key to confirm 42 9 6 RESET MODE SETTINGS The DNA Protein mode settings can be reset to their default values by pressing the function key below the Default option 9 7 DNA PROTEIN MEASUREMENTS WL 320 Bnm Abs DNA Protein Test 9 8 STORING METHODS RESULTS 26 Unm Abs DNA Protein Test Ensure that a cuvette containing the blan
16. RVE A standard curve can be saved to the instrument s WL 448 8nm Abs 6 26 i Calibration bahla 7 52 internal memory to allow it to be recalled for future use In the calibration table screen press the Save key on the instrument keypad The instrument will ask the user to enter a filename max 8 characters using the alpha numeric keypad The user can select the indicated letters numbers and symbols by repeatedly pressing the key until the required value is displayed after a short period the next character can be entered in the same manner Once the filename has been entered press the Enter key to confirm 6 6 RECALLING A STORED STANDARD CURVE A standard curve can be opened from the instrument s UL 448 8nm Abs 16 26 42 i Calibration tokio Nd ae internal memory to allow it to be re used for sample Do Smm UL nm quantification In the calibration table screen press the Open key on the instrument keypad The user can select from the list of stored files by pressing the up and down arrow keys ee Oo aL eU o o Once the required file has been located press the Enter WIE TANDARD Lit key to confirm 6 7 SAMPLE MEASUREMENTS The user should ensure that the appropriate standard curve data has been measured or recalled and that the instrument is displaying the quantitation mode screen before performing a sample measurement 31 6 7 1 Quantitation Measurements Ensure that a cuvette containing the bla
17. Y 6 168 50 eese rimen ES ect print putt 2 Select print output port Comm or Lpt following tasks 1 Reset printer printer 3 Select printer type nee 4 Change print mode Report or Screen Shot Highlight the required option using the up and down arrow keys and press the Enter key to confirm 19 4 3 3 Lamp Setup In the lamp setup menu the user can perform the UL Y nm E JENWAY AY 68 5i 0 following tasks wo aps Managenent 1 Switch on off the deuterium lamp u U 22 eset DZ abp usage time bag rs an 2 Reset the deuterium lamp usage timer 3 Switch on off the tungsten lamp 4 Reset the tungsten lamp usage timer 5 Change the lamp switch point Highlight the required option using the up and down arrow keys Press the Enter key to confirm 4 3 3 1 Change the Lamp Switch Point Highlight the Change the lamp switch point option and press the Enter key to confirm This will allow the user to enter the wavelength where the instrument will switch between the deuterium and tungsten lamps Enter a wavelength between 300 and 360 nm and press the Enter key to confirm Once complete the instrument will return to the Lamp Setup menu 4 3 4 Clock Setup In the clock setup menu the user can perform the WL 298 Unm o JENWAY 6 PE following tasks Turn 1 Settime EEE EX X 2 Set date Display time 3 Display time 4 Display date Highlight the required option using the up and dow
18. accessed by Calibration table gt pressing the function key below the Curve Fit option in 6 3 2 1 Select Curve Fit 440 Anm Abs 5 6 7 the quantitation mode screen Four new options are available in the quantitation table screen 4 Method Change the curve fit algorithm Params Enter linear regression constants Standard Edit calibration table data Show Curve Display calibration curve The curve fit algorithm can be selected by pressing the Calibration table pr function key below the Method option Four curve fit options are available Linear Fit Square Fit Quadratic Cubic Fit Linear Fit Through Zero The required option can be selected using the up and down arrow keys Press the Enter key to confirm 28 6 3 2 2 Manually Enter Curve Constants The user can manually enter the calibration curve WL 446 nm Abs Calibration table constants by pressing the function key below the No Conc a z Params option The user will be prompted to enter the constants that are required for the selected curve fit algorithm 1 Linear Fit KO K1 x Abs 2 Square Fit KO K1 x Abs K2 x Abs 3 Cubic KO K1 x Abs K2 x Abs K3 x Abs 4 Linear Fit Through Zero K1 x Abs The values can be entered using the numerical keypad Press the Enter key to confirm 6 3 2 3 Edit Calibration Table The user can edit the calibration curve data by pressing WI 140
19. al layout of the 6850 spectrophotometer is shown overleaf The light from the tungsten and deuterium lamps is focused onto the grating which separates the light into discreet wavelengths The diffracted spectrum of light then passes through a further slit and lens arrangement before passing through a beam splitter which directs half of the light towards to sample holder and half towards the reference sample holder The light which is not absorbed by the two solutions is transmitted through a collecting lens and onto the signal detector The signal from each photo diode detector is used to calculate the transmittance The result is displayed either as transmittance or absorbance on the instrument display 15 3 4 16 GOOD PRACTICE GUIDELINES 1 For optimum performance all spectrophotometers should be sited in a clean dry dust free atmosphere When in use ambient temperature and light levels should remain as constant as possible If required adherence to Standard Operating Procedures SOP and Good Laboratory Practice GLP should be monitored with regular calibration checks and a suitable Quality Control QC programme The sample chamber lid must be fully closed during measurement and before any readings are recorded or printed The correct selection of sample containers is imperative for accurate and reproducible results a Check that the material of the sample container is compatible with the wavelengths to be use
20. amber and insert the cuvette containing the sample solution into the sample position Close the instrument lid and the photometric result will be shown on the screen 5 4 2 Measuring a Sample after Entering a Concentration Factor WL 440 0nm 5 694 mg L Factor Mode Factor ste Remove the cuvette containing the blank solution and place a cuvette containing the sample to be measured in the sample chamber Close the instrument lid and the concentration value will be shown on the screen In order to measure a sample based on a known factor the value for the factor must be entered in the settings before commencing measurement of the sample 29 5 4 3 Measuring a Sample after Calibrating to a Standard 26 UL 140 8nm I 8615 35 99 mg L Factor Standard Conc 63 28 58 88 Remove the cuvette containing the standard sample and place a cuvette containing the sample to be measured in the sample chamber Close the instrument lid and the calculated concentration value will be shown on the screen SECTION 6 QUANTITATION The quantitation measurement mode enables sample concentrations to be calculated using a standard curve In this mode a number of standard solutions covering a range of known concentrations are measured The absorbance or transmittance of these solutions is plotted to create a standard curve Once the standard curve has been created a sample of unknown concentration can be measured
21. and the concentration calculated using the standard curve 6 1 QUANTITATION MODE SCREEN The quantitation measurement mode enables WL 446 Unm Abs 09 49 SAANS ee TOAS E measurement parameters to be changed The two Abs options at the bottom of the screen allow the user to select the unit of measurement and edit the calibration standard settings These options are selected with the corresponding function buttons 6 2 WAVELENGTH SELECTION The wavelength can be adjusted by pressing the GOTOA key on the instrument keypad The user can select from the following options 1 Single WL 2 Isobestic where Abs AbsA1 AbsA2 m x AbsA1 n x AbsA3 3 3 Points where Abs AbsA2 m n m AbsA3 AbsA2 and n AbsA2 Abs 1 The required wavelength values can then be entered using the numeric keypad Press the Enter key to confirm 6 3 METHOD SETUP In this measurement mode the method setup parameters are accessed by pressing the function keys below the two options on the quantitation menu screen 27 6 3 1 Selecting the Unit of Measurement The unit of measurement can be selected by pressing the function key below the Unit option The required unit of measurement can then be selected from the available options of IU mM L M L ug mL mg mL mg L mEq ppb ppm and other Press the Enter key to confirm 6 3 2 Quantitation Table Settings 4448 4nm Abs The quantitation table settings are
22. ate the calibration curve and to save results to USB memory stick In the Spectrum Kinetics Multi wavelength and DNA Protein measurement modes it is possible to save results to USB memory stick To open results from the USB memory stick on the instrument insert the USB memory stick when the instrument is in the home screen and enter the required measurement mode Press the OPEN button and scroll through the saved file names to the required result Press Enter and the result will open on the screen To open results from the USB memory stick in the PC software Prism select the corresponding measurement mode in the PC software From the File drop down menu select Open select the location of the USB stick memory stick and select the required result 47 SECTION 12 ACCESSORIES AND SPARE PARTS 11 1 OPTIONAL ACCESSORIES Part Code 685 204 685 131 685 005 685 304 685 401 035 088 035 091 060 422 035 143 685 035 685 040 Description of Accessory 10 x 10mm path length cuvette holder Water heated 10 x 10mm single cuvette holder 10 to 100mm adjustable path length cuvette holder Micro cuvette holder 8 position automatic cell changer Visible calibration set UV Visible calibration set Moulded cuvette rack for 16 10x10mm cuvettes Pack of 100 disposable micro cuvettes 70ul Prism PC software CD and security dongle Dust cover 11 2 INSTALLING THE PASSIVE AND ACTIVE ACCESSORIES There are two types of accessories which can be f
23. cell position is indicated below the lamp status icons in the top right hand corner of the screen To activate the accessory press the Cell key on the instrument keypad The indicated cell position will be highlighted on screen Each cell position is selected by pressing the corresponding digit on the numeric keypad e g position 6 is selected by pressing the number 6 key on the instrument keypad The instrument display will update to show the new automatic cell changer position Press the Cell key on the keypad to de activate the 8 position automatic cell changer 50 11 4 SPARES Part Code 685 020 685 021 Description of Spare Part Tungsten lamp Deuterium lamp 51 SECTION 12 MAINTENANCE AND SETVICE 12 1 ROUTINE MAINTENANCE Ensure the external surfaces of the unit are clean and free from dust The sample area should always be kept clean and any accidental spillage should be wiped away immediately To give added protection when not in use the unit should be disconnected from the mains supply and covered with the optional dust cover 12 2 LAMP REPLACEMENT Warning HOT Switch off the mains power supply and wait at least 20 minutes before attempting to replace the tungsten or deuterium lamps 12 2 1 Tungsten Lamp Replacement 1 Unscrew the 4 screws indicated 2 screws on each side and remove the cover of the spectrophotometer 2 Unscrew the 2 screws on the top of the lamp chamber and remove t
24. d for measurement In general glass can only be used down to 360nm or 320nm depending on quality Standard plastic cuvettes can be used down to 320nm Special UV versions can be used down to 260nm Below this level quartz cuvettes must be used b Plastic disposable cuvettes should only be used ONCE c Glass cuvettes should be thoroughly cleaned after use Discard when scratches become evident on optical surfaces d Care should be taken when selecting semi micro or micro cuvettes The cuvette window on the inner chamber the area filled with sample must be wider than the aperture in the sample holder or light will reach the detector without passing through the sample In this case semi micro or micro cuvettes with self screening black surrounds must be used or alternative holders for these cuvettes should be used e Glass test tubes and other sample tubes should be used with care Where possible matched tubes should be used and any index mark set to the correct position before measurements are made f Ensure any sample containers used are compatible with the constituents of both the samples and standards they are to hold Plastic cuvettes are not compatible with organic solvents g All sample containers must be handled with care by the top bottom and non optical surfaces only Any finger marks evident must be removed by a suitable cleaning process 10 11 h Flow through cuvettes must be selected with care and con
25. egolazione manutenzione e riparazione deve essere effettuata sulla base di quanto indicato nel presente manuale da personale qualificato consapevole dei rischi connessi fondamentale che il personale operativo e il personale addetto alla manutenzione utilizzino un sistema di lavoro sicuro oltre a seguire le istruzioni specificate nel presente manuale Oltre a quelli indicati nelle procedure di manutenzione all interno di questo dispositivo non sono presenti altri elementi sui quali possibile effettuare interventi La rimozione delle protezioni e qualsiasi tentativo di regolazione o di manutenzione posto in essere da personale non qualificato invalider la garanzia In questi casi sar necessario pagare un importo per le riparazioni effettuate 4 E sempre necessario fare riferimento ai dati sulla salute e sulla sicurezza forniti con le sostanze chimiche utilizzate Adottare le procedure di laboratorio generalmente accettate per la gestione delle sostanze chimiche Nel caso in cui si sospetti che la salute possa essere pregiudicata in qualsiasi modo disattivare l unit per renderla inutilizzabile Qualsiasi condizione di errore deve essere immediatamente segnalata al responsabile per la manutenzione Lea esta informacion atentamente antes de instalar o utilizar este equipo 1 La unidad descrita en este manual esta disenada para que solamente la utilice personal con formaci n Cualquier operaci n de ajuste mantenimien
26. etermined by WL 440 8nm I 16346 using an appropriate multiplication factor with the 0 014 me L measured photometric reading A concentration factor can be entered by pressing the function button below the Factor option The required factor can then be entered using the numerical keypad Press the Enter key to confirm 5 3 CALIBRATION 5 3 1 Zero Calibration TENUES A zero calibration can be performed at the same 000 Abs wavelength at which the sample will be measured With both the reference a sample positions empty and the sample chamber lid closed press the 100 T 0Abs button to zero the instrument s photometric reading 24 5 3 2 Calibrating to a Standard LC E3 03 40 SIMI A calibration standard can be used to determine the concentration factor Insert the cuvette containing the blank solution into the reference position in the sample chamber and insert the cuvette containing the standard solution into the sample position Close the instrument lid The concentration of the calibration standard is entered by pressing the function button below the Standard option The concentration of the calibration standard can then be entered using the numerical keypad Press the Enter key to confirm The concentration factor is then updated on the screen 5 4 SAMPLE MEASUREMENT 5 4 1 Photometric Measurements Insert the cuvette containing the blank solution into the reference position in the sample ch
27. f more than one result is stored in a file the up and down arrow keys can be pressed to scroll though the results Alternatively the user can press the left or right arrow key to enable a search option The number of the result that is to be viewed is entered using the numeric keypad Press the Enter Key to confirm 46 SECTION 11 SAVING RECALLING RESULTS USING A USB MEMORY STICK There is a USB port on the side of the instrument for data storage m The instrument only recognises a USB memory stick being inserted or removed ENWAY ME when it is operating in the home screen A 6850 USB icon appears at the top of the home Main Menu 5A screen circled in red MPhotometrics Mode A Quantitation Mode WSnectron SEE Insert the USB memory stick when the Kinetics Mode instrument is in the home screen and enter JDNA Protein Bode the required measurement mode Perform the ge EN cid measurement or measure the standards Ps EA Quantitation mode and press the SAVE button Enter the file name required using multiple presses of the numeric keys to select the required letter Press Enter to confirm the file name and the result is saved to the USB memory stick JENSAL Please note that in the Photometrics measurement mode the readout is live therefore results can t be saved to the internal memory of the instrument or to USB memory stick In the Quantitation measurement mode it is possible to save the data used to cre
28. he user can select from the list of stored files by pressing the up and down arrow keys Once the required file has been located press the Enter key to confirm SECTION 8 KINETICS The kinetics measurement mode enables the absorbance or transmittance of an active molecule to be measured over a period of time for example enzyme analysis of horseradish peroxidase The absorbance or transmittance is measured at regular time intervals at a set wavelength over a period of time The results are plotted on a graph to show the change in absorbance or transmittance over time Following sample measurement statistical analysis of all or part of the experiment can be performed 8 1 KINETICS MODE SCREEN UL 688 Gnm Abs Total T 3083 Intrvl 93 Operating Menu 8 2 WAVELENGTH SELECTION The kinetics measurement mode enables measurement parameters to be changed and post measurement tools to be accessed The four options at the bottom of the screen allow the user to edit the kinetics run settings select the measurement mode perform a linear regression analysis on the data and perform a post measurement search of the scan s data points These options are selected with the corresponding function buttons The wavelength can be adjusted by pressing the GOTOA key on the instrument keypad The required wavelength can then be entered using the numerical keypad Press the Enter key to confirm 8 3 METHOD SETUP WL 6
29. he cover 52 3 Whilst wearing cotton gloves remove the AS Tungsten defective tungsten lamp and replace with a Lamp E new one mut L 4 Switch on the power and move the Switch Mirror down into the position shown in the tat picture opposite Observing the entrance ne window on the instruments monochromator adjust the tungsten lamp until the light is centred on the entrance hole 5 Disconnect the power and re attach the lamp compartment cover and the top case of the spectrophotometer 1 Unscrew the 4 screws on the spectrophotometers top case see 12 2 1 and remove the cover of the spectrophotometer 2 Unscrew the 2 screws on the top of the lamp chamber and remove the cover see 12 2 1 3 Unscrew the 2 screws on the deuterium lamp flange No 1 unplug the connector on the power board No 2 and remove the deuterium lamp Whilst wearing cotton gloves replace with a new deuterium lamp Re fix the 2 screws and reconnect the power cable 4 Re attach the lamp compartment cover and the top case of the spectrophotometer 53 12 3 SERVICE Our dedicated service team are on hand to help in the unlikely event that your Jenway equipment develops a fault Please contact them by one of the following means with a clear description of the problem E mail service bibby scientific com Tel 44 0 1785 810475 Fax 44 0 1785 810471 On occasion it may be necessary for you
30. itted in the sample chamber passive or active accessories The range of passive accessories includes 10 x 10mm cuvette holders water heated cuvette holders adjustable path length 10 to 100 mm cuvette holders and micro cuvette holders The active accessory is an 8 position automatic cell changer The instrument must be turned off before any accessories are fitted 11 2 1 Passive Accessories Fixing lt SCTEWS 48 Unscrew the two fixing screws to undo the passive accessory Lift out the passive accessory To fit a different passive accessory place the accessory in the correct orientation and re tighten the two fixing screws to fix in place To replace the passive accessory with an active accessory refer to section 11 2 2 11 2 2 Active Accessories 11 2 2 1 8 Position Automatic Cell Changer Unscrew the two fixing screws to undo the passive accessory Lift out the passive accessory Fixing screws On the underside of the 8 cell accessory locate the accessories power supply and communication connector Accessory connector Take the 8 cell accessory and align the accessories connector with the instrument connection socket 49 Fixing screws a Place the accessory in the correct orientation and re tighten the two fixing screws to fix the 8 cell accessory in place 11 3 USING THE 8 POSITON AUTOMATIC CELL CHANGER When the automatic 8 cell turret is installed the current
31. k solution is in the reference position in the sample chamber and insert the cuvette containing the sample solution into the sample position Close the instrument lid and pres the Start Stop key on the keypad Once the measurement is complete the determined results will be displayed in the DNA Protein mode screen Further samples can be measured in a similar manner method result file can be saved to the instrument s internal memory In the DNA Protein mode screen press the Save key on the instrument keypad The instrument will ask the user to enter a filename max 8 characters using the alpha numeric keypad The user can select the indicated letters numbers and symbols by repeatedly pressing the key until the required value is displayed after a short period the next character can be entered in the same manner Once the filename has been entered press the Enter key to confirm 43 9 9 RECALLING STORED RESULTS method result file can be opened from the T 120 Onm Abs DNA Protein Test 2 Sam instruments internal memory In the DNA Protein mode screen press the Open key on the instrument keypad The user can select from the list of stored files by pressing the up and down arrow keys Once the required file has been located press the Enter key to confirm If more than one result is stored in a file the up and down arrow keys can be pressed to scroll though the results Alternatively the user can press the
32. lacement sis 53 123 SERVI aiii a 54 SEGION 19 TROUBEESPBOOTIN ou 55 131 TROUBLESHOOTING GUIDE std Lai nen 55 132 TECANICGAL SUPPORT reellen de il ea 55 SECTION 14 DECLARATION OF CONFORMITY eere 56 SECTION 1 Introduction 1 1 INSTRUMENT DESCRIPTION The 6850 is a variable bandwidth double beam UV visible spectrophotometer with an integrated user interface for local control This instrument has highly stable optics and two detectors that measure the sample and reference solutions simultaneously to optimise the accuracy of the measurement The 6850 has measurement modes for photometrics concentration multi wavelength spectrum scanning quantitation kinetics and DNA and Protein analysis The instrument is supplied with Jenway Prism PC software to allow full PC control of the spectrophotometer 1 2 INSTRUMENT SPECIFICATION GS Range Resolution Accuracy 0 3nm at 0 5 and 1nm bandwidth 0 5nm at 2 4 and 5nm bandwidth Repeatability Spectral bandwidth Transmittance Absorbance Accuracy 0 3 T 0 100 T 0 002A 0 0 5A Reproducibility 0 001 Abs 0 to 0 5 Abs 0 002 Abs 0 5 to 1 0 Abs Resolution Stray light Noise Stability 0 001A at 500nm after 15min warm up Range Data points Calibration Units Curve fit algorithms Multi wavelength Range Data points Measurement Time Up to 12 hours Scan Interval 0 1 0 2 0 5 1 2 5 10 or 30 seconds Calibration Blank with a single
33. lection ccecccccccsseeceecceeeeeceeeceeeeceeecsaaseceeeseeaseeeeseeeaeeeeseeaaeeeeesseaeeeeesssaaeeeees 21 4 3 9 Refresh System Baseline iii 21 4 3 10 Delete Entire Saved Files iii 22 4 3 11 Restore Default Settings sise 22 SECTIONS PROT OME TRIG do 23 5 1 PHOTOMETRICS MENU SCREEN coooocccccncccnccnnocccnnnnnnnnnnnanncnnnononononannnnnnnnononnnnnnennnnnnnnnnnans 23 5 2 METROOD SERUP M T E 23 5 2 1 Selecting a Wavelength sise 23 5 2 2 Selecting the Measurement Mode 23 5 2 8 Selecting the Unit of Measurement ses 24 5 2 4 Entering a Concentration Factor ss 24 5 9 einem E 24 5 3 1 Zerg Gallbralion RTT 24 5 3 2 Calibrating to a Standard sise 25 5 4 SAMPLE MEASUREMENT zn re nina ee see iE Ei 25 5 4 1 Photometric Measurements iii 25 5 4 2 Measuring a Sample after Entering a Concentration Factor cccccccecsseeeeeeeeeeeeaeeeeeens 25 5 4 3 Measuring a Sample after Calibrating to a Standard ccccconccnccconcncononcnnononcnncnnanoncnnanenons 26 SECTION G QUANTITA FION ee ae ee none 27 6 1 QUANTITATION MODE SCREEN geenien e i a a 27 6 2 WAVELENGTH SEEEG FON sen en nee x urb nos 27 6 3 METHOD ES EP UU 27 6 3 1 Selecting the Unit of Measurement ss 28 6 3 2 Quantitation Table Selina 28 6 3 2 1 Select QUO lla TT mo T mm 28 6 3 2 2 Manually Enter Curve Constant oooccnccccconccnncccnonccnncnnnnnnnnnnnnnnncnnnnnnnnncnnnnnnnnncnnnn
34. ll be heard each time an instrument button is pressed when this option is enabled 4 3 8 Language Selection Not currently available 4 3 9 Refresh System Baseline Ensure the sample chamber is empty highlight the refresh system baseline option and press the Enter key to confirm This will re measure and store the detectors signals over the full wavelength range of the instrument 21 4 3 10 Delete Entire Saved Files Highlight the delete entire saved files option and press the Enter key to confirm A warning will be shown on screen and the user will need to confirm this command again by selecting the Yes option with the up and down arrow keys This action will delete all saved results and methods from the instrument 4 3 11 Restore Default Settings Highlight the restore default settings option and press the Enter key to confirm This will reset all instrument parameters back to their original factory settings 22 SECTION 5 PHOTOMETRICS The photometrics measurement mode enables simple measurements of absorbance transmittance and concentration to be performed In this measurement mode it is possible to calibrate against a standard of a known concentration or use a known factor The sample is measured at one wavelength at one point in time There are no post measurement calculations available in this measurement mode 5 1 PHOTOMETRICS MENU SCREEN The photometrics measurement mode screen enables WL 446 Mn 0 0
35. measurement mode enables measurement parameters to be changed The four options at the bottom of the screen allow the user to edit the concentration calculation factors select the measurement mode select the units of the measurement and reset the modes settings to their default values These options are selected with the corresponding function buttons 9 2 ADJUSTING THE MEASUREMENT MODE 14 08 54 FEL Y ee WL nm 260 0 280 0 330 0 Eu PERTE sorbance difference 1 The DNA Protein measurement mode includes two sets of calculations to calculate the concentration of nucleic acid and protein samples The measurement mode option can be selected by pressing the function key below the Mode option The options that are available are 1 Absorbance Difference 1 2 Absorbance Difference 2 The default equations and factors used by the instrument are shown below Absorbance Difference 1 Purity Ratio Abs 260nm Abs 280nm DNA Conc pg ml Abs 260 x 62 9 Abs 280 x 36 0 Protein Conc ug ml Abs 280 x 1553 Abs 260 x 757 3 41 Absorbance Difference 2 WL 328 Gnm Abs n Test Purity Ratio Abs 260nm Abs 230nm RNA Conc ug ml Abs 260 x 49 1 Abs 230 x 3 48 Protein Conc ug ml Abs 230 x 183 Abs 260 x 75 8 The option to include the third wavelength as a reference is given after the measurement mode is selected Use the up and down arrow keys to select Yes or
36. n arrow keys Press the Enter key to confirm 4 3 4 1 Set Time Highlight the set time option and press the Enter key to confirm This will allow the user to enter the current time using the numerical keypad and the decimal point keys Press the Enter key to confirm Once complete the instrument will return to the Lamp Setup menu 20 4 3 4 2 Set Date Highlight the set date option and press the Enter key to confirm This will allow the user to enter the current date in the format DD MM YY using the numerical keypad and the decimal point keys Press the Enter key to confirm Once complete the instrument will return to the Clock Setup menu 4 3 4 3 Display Time Date Highlight the display time or display date option and press the Enter key to confirm This will set the top right hand corner to show the selected option 4 3 5 Refresh Dark Current Highlight the refresh dark current option and press the Enter key to confirm This will re measure and store the detectors dark current values 4 3 6 Connect to PC Connect the interface cable to the USB type B port on the rear of the instrument and connect to a USB port on the PC The connecting COM port should be configured with the following settings 38400 baud 8 data bits No parity 1 stop bit Highlight the Connect to PC option and press the Enter key to confirm 4 3 7 Beeper On Off Highlight the beeper on off option and press the Enter key to confirm An audible sound wi
37. n be selected by pressing the function key below the Mode option The required measurement mode can then be selected from the available options of Abs and T using the up and down arrow keys Press the Enter key to confirm 7 4 SAMPLE MEASUREMENTS 658 8nm Abs Insert a cuvette containing the blank solution into the reference position in the sample chamber and insert the cuvette containing the sample solution into the sample position Close the instrument lid and press the Start Stop key on the keypad Once the measurement is complete the measured spectrum scan will be displayed on the screen 7 5 ADJUSTING THE DISPLAYED SCAN RANGE 658 Bnm Abs 230 0 Wavelength nn Min X 500_ 34 The instrument will display the full range scan after a measurement is complete To change the displayed wavelength range press the left or right arrow keys The minimum and maximum display wavelengths can be entered using the numeric keypad Press the Enter key to confirm To change the displayed photometric range press the up or down arrow keys The minimum and maximum values can be entered using the numeric keypad Press the Enter key to confirm The spectrum scan will then be shown with the updated range values To return to the full spectrum scan the user will need to re enter the original wavelength and photometric range values 7 6 SPECTRUM SEARCH ie j avelength nm 7 7 SPECTRUM SMOOTHING 60 Unm Abs E
38. netics netics Node petrae eu DNA Protein and multi wavelength The system utility menu option allows the user to perform a variety of setup and service functions See section 4 3 for further details on these Main Menu Screen options 4 2 1 Slit Width Setting The slit width option allows the user to select the instrument s bandwidth parameter from the available options 0 5 1 0 2 0 4 0 and 5 0 nm Use the arrow keys to cycle through the options and press the Enter key to confirm The instrument will perform a dark current calibration before returning to the main menu 18 4 3 SYSTEM UTILITY MENU The system utility menu option allows the user to WL 238 8nm I perform a variety of setup and service functions JENWAY 685 6850 1 Wavelength calibration ce Routines 1 OPE Reset rinte 2 Edit printer settings etup 3 Editlamp settings 4 Edit time and date settings 5 Measure the instrument s dark current 6 Connect to a PC 7 Edit keypad sound options 8 Setthe displayed language 9 Perform a system baseline measurement 1 0 Perform an instrument reset System Utility Menu 4 3 1 Wavelength Reset Highlight the wavelength reset option and press the Enter key to confirm This will recalibrate the wavelength setting of the instrument Once complete the instrument will return to the System Utility menu 4 3 2 Printer Setup In the printer setup menu the user can perform the WI JU Unm I JENWA
39. nk solution is in 140 Bnm Abs Quantitation Mode TE the reference position in the sample chamber and insert the cuvette containing the sample solution into the sample position Close the instrument lid and pres the Start Stop key on the keypad Once the measurement is complete the determined result will be displayed in the quantitation mode screen Further samples can be measured in a similar manner 6 8 STORING RESULTS A result file can be saved to the instrument s internal Tu memory In the quantitation mode screen press the Save key on the instrument keypad The instrument will ask the user to enter a filename max 8 characters using the alpha numeric keypad The user can select the indicated 7 0 058 8 294 A 1 x 0 995 IE letters numbers and symbols by repeatedly pressing lease input File Name 51DRI the key until the required value is displayed after a short period the next character can be entered in the same manner Once the filename has been entered press the Enter key to confirm 6 9 RECALLING STORED RESULTS A result file can be opened from the instrument s WL 418 0nm Abs 16 24 10 internal memory Quantitation Mode D 2 Ss CE Sins In the quantitation mode screen press the Open key on the instrument keypad The user can select from the list of stored files by pressing the up and down arrow keys Once the required file has been located press the Enter key to confirm
40. omoothin Spectr 7 8 STORING RESULTS 33 input File Name SPECTRUM_ The photometric values of the measured spectrum can be viewed by pressing the function key below the Search option The user can press the up and down arrow keys to move between the peaks in the scan that are above the threshold peak height The user can alternatively press the left or right arrow keys to review each point in the scan in turn The peak height threshold can be adjusted by pressing the function key below the PK Height option The threshold peak height value can be entered using the numeric keypad Press the Enter key to confirm The measured spectrum scan can be smoothed by pressing the function key below the Smooth option A result file can be saved to the instrument s internal memory Press the Save key on the instrument keypad The instrument will ask the user to enter a filename max 8 characters using the aloha numeric keypad The user can select the indicated letters numbers and symbols by repeatedly pressing the key until the required value is displayed after a short period the next character can be entered in the same manner Once the filename has been entered press the Enter key to confirm 35 7 9 RECALLING STORED RESULTS mu O O ce 230 0 Wavelengthi nm se ew Ess PCT 36 A result file can be opened from the instrument s internal memory Press the Open key on the instrument keypad T
41. onanncnnnnnnnas 29 6 9 2 9 Edit GalibEBton Table xi itio ret E UE epo an 29 6 3 2 4 Display Calibration Curve RL nennen nennen nennen nnn nnns 29 6 4 CREATING A NEW STANDARD CURVE cooocccccccccnncnccccnnnccnnnnconccnnnnnnnnnnnnnnnnnnnononnnnanennnnnnns 30 6 5 STORING A STANDARD CURVE nn 31 6 6 RECALLING A STORED STANDARD CU URVE ins 31 6 7 SAMPLE MEASUREMENT S cta 31 6 7 1 Quantitation Measurements iii 32 6 8 STORING RESUE Sat a E 32 6 9 REGAELING STORED RESULTS neck 32 SEGSTIONF ZSPECTRUN see dues eee eee ane 33 7 1 SPEGTRUMMODESEREEN eek 33 7 2 METHOD SETUP SSSR a set 33 7 3 SELECTING THE MEASUREMENT MODE u ns sde 34 7 4 SAMPLE MEASUREMENTS gen nd du duda fa das 34 7 5 ADJUSTING THE DISPLAYED SCAN RANGE cesses ek ne nnac 34 7 6 SIPEG TRUM SEARS dida 35 TT SPEGTRUN SMOOTHING a een 35 7 8 STORING RESULT Sa roca 35 7 9 RECALLING STORED RESULTS 36 SEOTION eg 0 komad OS ee eee 3 8 1 KINETICS MODE SCREEN sauer 37 8 2 WAVELENGTH SELECTHON ES Eee 37 8 3 METHOD SETUP a 37 8 3 1 Selecting the Measurement Mode ss 38 8 4 SAMPEE MEASUREMENTS lt a 38 8 5 ADJUSTING THE DISPLAYED SCAN RANGER ins 38 8 6 CALCULATING VU SONGENTRATION ana 39 8 7 SPECTRUM SMOOTHING alaba 39 8 8 STORINGIRE SULTS arisna Li rican eee Qert ae 40 8 9 RECALLING STORED RESULTS eee 40 SEG TIONS DNA PROTEIN Essen 41 9 1 DNA MENU OPTION Siria aaa 41 9 2 ADJUSTING THE MEASUREMENT MODE iii 41 9 3 WAVELENGTH SEBEGTIODN lt td 42
42. onnes form es Tout r glage maintenance ou r paration doit tre effectu comme d crit dans ce manuel par une personne qualifi e consciente des risques encourus Il est essentiel que les personnes utilisant et intervenant sur cet appareil respectent les r gles de s curit de travail en plus des instructions d taill es pr cis es dans ce manuel En dehors des l ments d crits dans les proc dures de maintenance ci incluses cet appareil ne contient aucun l ment r parable par l utilisateur L enl vement des capots et les tentatives de r glage ou de r paration par des personnes non qualifi es invalide toute garantie et entra ne un risque de frais de r paration suppl mentaires Toujours se r f rer aux fiches techniques de sant et de s curit accompagnant tout produit chimique utilis Respecter les proc dures de laboratoire g n ralement accept es pour la manipulation en toute s curit des produits chimiques Si l utilisateur suspecte qu un probl me quelconque puisse mettre en cause la s curit l appareil doit tre rendu inop rant en emp chant son utilisation Communiquer la d faillance constat e au service de maintenance comp tent Bitte lesen Sie diese Hinweise vor Installation oder Gebrauch dieser Ausrustung sorgfaltig durch 1 Das in diesem Handbuch beschriebene Ger t darf nur von geschultem Personal bedient werden Alle Anpassungen Wartungsarbeiten und Reparaturen m ssen entsp
43. r equipment to be sent back to our Service Department for repair In this case please contact the Service Department for a reference number which you should include with your faulty equipment Please also ensure you include a clear description of the fault and a completed copy of our Decontamination Certificate This is available as a downloadable pdf file at www jenway com or contact us and we will be happy to fax you a copy Please clearly mark the package for the attention of the Service Department and post to the following address Bibby Scientific Ltd Beacon Road stone Staffordshire ST15 OSA United Kingdom All replacement parts are guaranteed for 1 year and where ever possible returned equipment is turned around in 10 working days 54 SECTION 13 TROUBLESHOOTING 13 1 TROUBLESHOOTING GUIDE Unable to achieve a reading when Ensure the correct cuvettes are being used so that light measuring a sample isn t being absorbed by the cuvette Ensure the sample isn t too dense that light is not transmitted through the sample Ensure the lamp is working 13 2 TECHNICAL SUPPORT Jenway have a dedicated Technical Support team made up of experienced scientists who are on hand to help with any applications advice and questions you may have about our products and how to use them If you require any technical or application assistance please contact the team at E mail jenwayhelp bibby scientific com Phone 44 0 1785 810433
44. r lid Sample chamber Fig 2 6 Front Panel 13 SECTION 3 THEORY AND PRACTICE OF SPECTROSCOPY MEASUREMENTS 3 1 THEORY OF SPECTROSCOPY MEASUREMENT UV visible spectroscopy is the measurement of the absorbance of light at a specific wavelength in a sample This is used to identify the presence and concentration of molecular entities within the sample The Beer Lambert law is used to relate the absorption of light to the properties of the sample through which the light is travelling through The Beer Lambert law states that A elc is the absorbance A t is the molar absorption coefficient I mol cm C is the concentration mol I is the path length cm This law shows that absorbance is linear to concentration but this is only true for low concentrations For absorbance levels above 3 the concentration starts to move away from the linear relationship Transmittance is the proportion of the light which passes through the sample Where lo is the incident light h is the transmitted light L is the path length L Therefore T h lo Absorbance is inversely related to transmittance A log 1 T 14 3 2 NUCLEIC ACID DETERMINATION DNA RNA and oligonucleotides can be measured directly in aqueous solutions in a diluted or undiluted form Aqueous buffers with low ion concentrations e g TE buffer are ideal for this method The concentration is commonly determined by measuring at 260nm against a blank and
45. rechend der Vorgaben in diesem Handbuch und von einer kompetenten Person die mit den damit verbundenen Gefahren vertraut ist durchgef hrt werden Es ist wichtig dass sowohl das Bedienungs als auch das Service Personal zus tzlich zu den detaillierten Anweisungen in diesem Handbuch ein sicheres Arbeitssystem einsetzen Mit Ausnahme der Teile deren Wartungsverfahren in diesem Handbuch beschrieben sind enth lt dieses Ger t keine weiteren Teile die vom Benutzer gewartet werden k nnen Das Entfernen von Abdeckungen und Versuche von hierf r unqualifiziertem Personal Anpassungen oder Wartungsarbeiten durchzuf hren haben zur Folge dass die Garantie verf llt und k nnen zus tzliche Reparaturkosten ausl sen Es ist jederzeit auf die sicherheitsrelevanten Daten s mtlicher verwendeter Chemikalien Bezug zu nehmen Allgemein anerkannte Labormethoden zum sicheren Umgang mit Chemikalien sollten eingesetzt werden Besteht der Verdacht dass die Sicherheitsvorrichtungen in irgendeiner Weise besch digt wurden muss das Ger t au er Betrieb genommen und gegen weiteren Gebrauch gesichert werden Die St rung sollte der zust ndigen Serviceeinrichtung unverz glich gemeldet werden Leggere attentamente queste istruzioni prima di installare o utilizzare il dispositivo 1 L unit descritta nel presente manuale stata realizzata per essere utilizzata solo da personale che ha ricevuto l apposita formazione Qualsiasi operazione di r
46. s 8 1 2 INSTRUMENT SPECIFICATION ccccccccccccccccceeeccccccccccsececscccescecesseecetteuesececsueeerttneeeeeeeens 8 SECTION 2 Installation cc cccccccccccccccccccccccecuccecucuecccuucuuuuaneneaueaesuusueeauecusuesusunanes 9 2 1 Wiz ie ICE dl dal dl dal it 9 2 2 NR AA 9 2 3 ASI A A 10 2 4 ONT Eo ne a todas desearte 11 2 5 REAR PANEL un ee es ne de PR tre ee dr nn a 13 2 6 FRONT VIEW es 13 D O EE den de De De a D D TE E E 14 3 1 THEORY OF SPECTROSCOPY MEASUREMENT cccccccceessseeeeeeeeeeeeaeeeeeeeeeeeeeeaas 14 3 2 NUCLEIC AGID DETERMINATION ESS ES de n es Seva dais 15 3 3 SPEGTHOSGOPY MEASUREMEN Esad ne demie 15 3 4 GOOD PRAG TICE GUIDELINES solides Messen a lee 16 SECTION 4 INSTRUMENT SETUP serres 18 4 1 INSTRUMENT NAVIGATION esse a ee ee een 18 4 2 MAIN MENU SCREEN see ee ee Beeren een 18 42 1 SIE WIN SEI Ng anne 18 4 3 SYSTEM UTIEITYMEN Dessauer ee 19 4 31 Wavelength HeSQl oos puce rome nn een 19 432 PANE MS LS LE Ze 19 433 Lamp seso m et 20 4 3 3 1 Change ihe Lamp Switch Point ressrinsaanta sn A A a 20 oo lens ea dnt eA EAA a 20 n MES WEIN EU o LLL 20 AS Dale nenn 21 4 34 3 Display Liner D le ass 21 435 PereshDAK CUNEN Jennerien eea faena io eda pta aeeoes at eu ense polo puto iae 21 A N a tarot Ness nn die rise 21 4 3 7 Beeper On Off ccccccccssssscceccceseceecceeesececcsseuseceecseaueceeesseaseceesseuaeeeeesseageeeeessauseeeesssagseeess 21 4 3 8 Language Se
47. s can be entered using the numeric keypad Press the Enter key to confirm To change the displayed photometric range press the up or down arrow keys The minimum and maximum values can be entered using the numeric keypad Press the Enter key to confirm The kinetics scan will then be shown with the updated range values The return to the full kinetics scan the user will need to re enter the original time and photometric range values 8 6 CALCULATING I U CONCENTRATION The instrument software allows the user to calculate the concentration of a substance from the difference between the photometric values at two time points in the displayed kinetics scan To enter the calculation parameters press the function key below the Process option and enter a Start Time Time s qe an End Time and a Factor pressing the Enter key to 0 Start Timwe 10_ confirm each value When all values have been confirmed the instrument will calculate the concentration value according to the following equation Conc 1 0 AbS start time ADS end time x Factor The calculated concentration value will be displayed on the kinetics mode screen Time s U Setup Mode Process See 8 7 SPECTRUM SMOOTHING The photometric values of the measured kinetics scan can be viewed by pressing the function key below the Search option The user can press the up and down Total T 308 arrow keys to review each point in the scan in turn
48. sideration for the sample type sample volume pumping system rinse sample and waste handling to be used Samples and standards should not be stored in open cuvettes or sample containers as evaporation will change the value and lead to staining of the walls which may be irreversible If stored in stoppered and sealed cuvettes they should be filled with little or no air space and the values regularly checked against a reference standard or quality control material Samples should be allowed to equilibrate to ambient temperature before measurement unless a suitable temperature controlled sample holder is in use Temperature change during measurement may cause air bubbles to form on the walls of the sample holder This is acommon cause of drift during measurement In the preparation of samples and standards high grade borosilicate glass and AR grade chemicals and reagents must be used Good quality deionised water or other suitable solvents must be used for dissolving or diluting samples chemicals and reagents All measurements require calibration to a blank for maximum accuracy this should be prepared with care using the same deionised water or solvent used for dissolving or diluting the sample Where reagents are added to the sample to produce a colour proportional to its concentration a sample based blank should be used In this case the blank should consist of all reagents or chemicals to be used except the sample which will
49. then evaluating against a factor The 6850 has pre defined methods installed which assume that absorption of 1 OD A is equivalent to approximately 50ug ml dsDNA 37ug ml ssDNA 40ug ml RNA and 30ug ml for oligonucleotides DNA interference by contaminants can be assessed by the calculation of an absorption ratio The ratios A260 A280 and A260 A230 are used to estimate the purity of nucleic acids since proteins absorb at 280nm and substances such as peptides phenols aromatic compounds or carbohydrates absorb at 230nm Pure DNA should have an A260 A280 ratio of approximately 1 8 and pure RNA 2 0 In pure nucleic acid samples the A260 A230 ratio should be approximately 2 2 Nucleic acid concentration can also be estimated with the following calculations Conc ug ml Abs 260nm x 62 9 Abs 280nm x 36 0 Conc ug ml Abs 260nm x 49 1 Abs 230nm x 3 48 Referring to a blank value where no absorption should occur is commonly required On the 6850 the default reference wavelength is 320nm and the user can include the measured absorbance value in all nucleic acid calculations The default wavelength can be modified from 320nm if required 3 3 SPECTROSCOPY MEASUREMENT There are four main components of a spectrophotometer These are a light source to emit a high and constant amount of energy over the full wavelength range a method for separating the light into discreet wavelengths a sample holder and a light detector The optic
50. to y reparaci n debe llevarse a cabo del modo indicado en este manual y debe realizarla una persona cualificada que sea consciente de los peligros que implica Es fundamental que tanto los operarios como el personal de servicio utilicen un sistema de trabajo seguro as como las instrucciones detalladas que se especifican en este manual Cualquier elemento que no se encuentre entre los definidos en los procedimientos de mantenimiento aqu descritos no podr utilizarse en este instrumento La extracci n de las tapas y los intentos de ajuste o reparaci n por parte de personal no cualificado invalidar n la garant a y pueden incurrir en cargos adicionales por reparaci n Siempre deber an consultarse los datos sobre Salud y Seguridad que se suministran con cualquier producto qu mico que se utilice Es necesario llevar a cabo los procedimientos de laboratorio de aceptaci n generalizada para la manipulaci n segura de productos qu micos Si existe la sospecha de que las medidas protectoras de seguridad han quedado da adas en cualquier modo la unidad debe inutilizarse y protegerse contra toda operaci n que se intente llevar a cabo El estado de fallo debe comunicarse inmediatamente a la autoridad de servicio de mantenimiento y reparaci n pertinente Contents SEGCTHON TEIIHTOAUCH ON SE SR eho eed eed a ee ee Ead 8 1 1 INSTRUMENT DESCRIPTION ccccccccccccccccccecceccccceecccsececsesuescecessecertuuesecessueeerttseeeeeeeeen
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