Vinmetrica SC-100A™ User Manual
Contents
1. Vinmetrica SC 100A Uinmetnica User Manual The Vinmetrica SC 100A is a simple and robust device that provides high accuracy in determination of sulfite SO2 levels in wine These are essential parameters to control in the effort to make high quality wines Materials provided in the kit 1 Vinmetrica SC 100A SO controller unit Part number SC100A 1 SO Electrode Part number SC 100 3 blue polycarbonate housing SO Reagent set Part number SC 100 2 SO Titrant solution 0 0156N Acid Reagent Reactant solution One 5 mL syringe Two 3 mL polyethylene transfer pipettes One 25 mL serological pipette One 100 mL polypropylene beaker a i ee a Things you will need Figure 1 The SC 100A instrument 1 Two standard AA batteries alkaline type 2 Distilled water which usually can be found at your local grocery store Its handy to have a wash bottle for rinsing Rinse bottle available from Vinmetrica Part number SC 100 17 3 Optional Sodium Hydroxide solution 1N concentration 1f you want to do total SO Available from Vinmetrica Part number SC 100 7 http vinmetrica com wine analysis products 4 Optional Deluxe Lab Accessory Kit which includes magnetic stirrer burette 1OmL or 25mL lab support stand and electrode holder Available from Vinmetrica Part Number SC 300 9 https vinmetrica com product the deluxe lab accessory kit US and international patents pending SC 100A manual 1 Versi2. 64 mg SO mmol SO V mL N meq mL 1000 mL L 2 meq mmol SO S mL SC 100A manual 6 Version 2 1c WARRANTIES AND LIABILITIES l The materials provided in the kit as described on pages 1 and 2 above Materials are warranted as follows The SC 100A instrument electrode and non reagent accessories are warranted against defects in workmanship for 24 months from date of purchase The reagents are warranted to perform as described herein up until any stated expiration date or 6 months after purchase whichever is later THE WARRANTIES IN THESE TERMS AND CONDITIONS ARE IN LIEU OF ALL OTHER WARRANTIES EXPRESS OR IMPLIED INCLUDING WITHOUT LIMITATION ANY WARRANTIES OF MERCHANTABILITY NONINFRINGEMENT OR FITNESS FOR A PARTICULAR PURPOSE SAID WARRANTIES BEING EXPRESSLY DISCLAIMED 2 Buyer agrees that its sole and exclusive remedy against Vinmetrica shall be limited to the repair and replacement of Materials or parts of Materials provided Vinmetrica is promptly notified in writing prior to the expiration of the warranty period specified above of any defect Vinmetrica s liability for any damages due Buyer shall be limited to the purchase price of the Materials 3 VINMETRICA S MAXIMUM LIABILITY FOR ALL DIRECT DAMAGES INCLUDING WITHOUT LIMITATION CONTRACT DAMAGES AND DAMAGES FOR INJURIES TO PERSONS OR PROPERTY WHETHER ARISING FROM VINMETRICA S BREACH OF THESE TERMS AND CONDITIONS BREACH OF WARRANTY NEGLIGENCE STRI
3. displayed For values under 10 one 500 kOhm resistor decimal place is shown 5 pH Voltage pH probe or precision The raw voltage output from the voltage source Do instrument s pH amplifier is displayed as not exceed 0 5 V X XX volts Readings can range from 0 00 to 4 10 SC 100A manual 8 Version 2 1c zm Seton Equipment Required 6 SO 2 Voltage SO probe or The raw voltage output from the SO probe simulator instrument s current amplifier is displayed as X XX volts X XX if less than 1 00 Disconnect probe cycled through its 32 levels Note Ignore red LED error indication 8 Battery Install two AA The battery voltage is displayed as X XX Voltage batteries volts 9 Character None Every ASCII character space to 1s 7 DAC Test None The Digital Analog Converter DAC is Set displayed Due to the limitations of the 7 segment format some characters are not used by the software Display every possible digit and every decimal point s 11 Sound Test The beeper is turned on continuously 12 pH CAL None only firmware Displays current CAL values for pH 7 values v3 0 6 and later and 4 in mV Pressing ENTER cycles between these If a pH CAL reset has been done displays CAL value for pH 3 rather than 4 13 pH CAL None only firmware Displays PrESS EntEr Press ENTER reset v3 0 6 and later to reset pH CAL parameters to default values Message Good CAL rSt then scrol
4. contaminate the transfer pipettes If they do get contaminated rinse them off with distilled water and let air dry Caution the Acid reagent is corrosive and can cause damage to clothing skin and eyes All of the reagents are not to be ingested We recommend use of laboratory safety glasses and latex or nitrile gloves during this procedure If any solutions contact skin or eyes flush with plenty of water 5 Rinse the electrode briefly with distilled water before inserting it into your wine sample 6 If you are stirring manually begin now use a constant moderate swirling motion If the electrode is not held in a stand hold it against the side of the vessel with one finger and grasp the vessel with the remaining fingers so that the two move together Figure 7 7 If you are using a magnetic stirrer turn it on to stir at a moderate rate Be sure the stir bar will not strike the electrode in the next step Insert the electrode into the titration beaker so that the tip is completely submerged to just above the circulation gaps cutouts at the tip of the electrode but above the level of the stir bar approximately a half an inch from the bottom of the titration beaker If you are using the Vinmetrica Electrode Holder adjust it to a similar level 8 Verify that the current is less than 50 and the green PROCEED LED is lit If the current is greater than this and or the red STOP LED is lit and the buzzer sounds your sample has l
5. 4 V N 1000 ppm SO S Where V mL Titrant used to reach the endpoint N normality of the Titrant S mL sample If you use 25 mL of sample as directed and the Titrant is 0 0156 N as supplied then the calculation is simply ppm SO 20 V ie 20 times V Measuring Total SO by Titration optional requires IN NaOH 1 Place 25 mL wine or must in the titration vessel See Figure 5 2 Add 10ml IN sodium hydroxide Vinmetrica Part number SC 100 17 and mix well Let stand approximately 10 minutes 3 Using the transfer pipettes add approximately 8 mL of the Acid Reagent and 2 mL of the Reactant solution to the vessel Remember that if you are using the transfer pipettes in the kit 2 mL is marked on the side of the neck of the transfer pipette so dispense four of these for the Acid Reagent 4 Proceed from step 5 in the Free SO2 procedure above The result calculated will be total SO rather than free SO in parts per million ppm Finishing up 1 Turn off the instrument and unplug the electrode 2 Rinse the SO electrode and syringe with distilled water Let air dry 3 Store all reagents tightly capped and away from heat and sunlight 4 Discard waste samples and solutions in accordance with local regulations Acidic solutions can be neutralized by slow addition of baking soda sodium bicarbonate with stirring until effervescence ceases Technical assistance info vinmetrica com tel 760 494 0597
6. CT LIABILITY OR OTHER TORT WITH RESPECT TO THE MATERIALS OR ANY SERVICES IN CONNECTION WITH THE MATERIALS IS LIMITED TO AN AMOUNT NOT TO EXCEED THE PRICE OF THE MATERIALS IN NO EVENT SHALL VINMETRICA BE LIABLE TO BUYER FOR ANY INCIDENTAL CONSEQUENTIAL OR SPECIAL DAMAGES INCLUDING WITHOUT LIMITATION LOST REVENUES AND PROFITS HAZARDS AND TOXICITY All Materials offered by Vinmetrica are intended for use by individuals who are familiar with laboratory procedures and their potential hazards The Materials contain chemicals which may be harmful if misused Due care should be exercised with all Materials to prevent direct human contact Glassware can break and chemicals can splash during experiments Always use safety glasses We strongly recommend using nitrile or latex gloves and wearing long pants long sleeves and closed toed shoes Keep out of reach of children Viametuca 1945 Camino Vida Roble Suite Carlsbad CA 92008 www vinmetrica com 760 494 0597 info vinmetrica com Copyright 2010 14 Sportsman Consulting LLC DBA Vinmetrica All rights reserved SC 100A manual 7 Version 2 1c Appendix A Test Mode Test Mode provides various special functions that may be useful in testing the device for example if troubleshooting is necessary Test Mode is entered when the POWER button is pressed longer than 2 seconds while turning the instrument on Remove the pH electrode 1f it is attached e Test Mode is organized into sect
7. ake sure the outside of the syringe is dry to minimize inaccuracies Note the 5 0 mL setting allows determination of up to 100 ppm SO in a standard 25 mL wine sample If you are using a buret instead fill it now Figure 4 Withdraw Place 25 mL wine or must in the titration vessel Figure 5 We recommend the titrant from its using the 25 mL pipette provided in the kit draw sample up to the 0 mL mark bottle using a clean 5 mL syringe then dispense the sample into your titration vessel by letting the tip of the pipette a touch the side of the vessel while the sample drains NEVER pipette any reagents by mouth Also make sure the pipette you are using is completely clean before submerging into your wine sample Do not use sulfite to sanitize it Use 25 ethanol or other sterilant Figure 5 Dispense 25 mL of your wine into the titration beaker using the 25 mL sampling pipette Make sure this is clean before putting the pipette into your wine container SC 100A manual 4 Version 2 1c 4 Using the transfer pipettes add about 2 mL Acid Solution and 2 mL Reactant solution to the titration beaker Figure 6 the two transfer pipettes should come with red and yellow labels with red for the Acid Reagent and yellow for the Reactant It is not necessary to be extremely accurate in this step with these pipettes 2 mL indicated on the neck of the pipette To preserve the shelf life of these reagents take care not to cross
8. cess See Appendix B for more information on how to adjust your wine for sulfite Theory of operation Sulfite S02 The SC 100A with the SO electrode and reagents provided can be used to determine sulfite or SO2 levels in wine musts and other samples It relies on the Ripper titration based on the quantitative reaction of the SO with iodine generated during the titration which oxidizes the SO gt in the sample under acid conditions IO 51 6H gt 3L 3H O generation of iodine from iodate SO bL H2O gt 21 SO 3 2H reaction of SO and iodine When all the SO is titrated at the endpoint excess iodine appears in solution This is detected as current with the SO electrode and signaled by audible and visual indicators The endpoint is much more sensitive than the starch color change commonly employed for Ripper titration and it is sharp and clear even when titrating red wines and musts From the known concentration of the titrant and its volume required to reach the endpoint the free SOz is simply calculated SC 100A manual 2 Version 2 1c Setup Setting up the SC 100A for the first time 1 The SC 100A Figure 1 runs on two standard AA batteries alkaline cells recommended To insert the batteries open the battery housing on the bottom of the back of the unit by removing the two screws and gently prying off the lid Install the batteries the springs are the terminals and the metal contacts are the ter
9. ess than 2 ppm SO and there is no need to proceed 9 Titrate the sample by adding the SO2 Titrant dropwise from the syringe or burette being sure to note the starting volume mark on the syringe or burette Figure 7 Try to accomplish the titration as rapidly as possible in 2 minutes or less but be careful near the endpoint so as not to overrun it here Figure 7 Manual stirring dispense one or two drops at a time Be technique Hold the electrode against the side of the titration beaker and swirl gently add SO2 throughout the entire procedure Firat oa other hand sure to maintain stirring or swirling 10 During the titration the LCD display will show transient currents the red STOP LED will briefly illuminate and the beeper will sound beep beep These transient indicators will last longer and longer as you approach the endpoint Take the endpoint as the first addition of Titrant that causes the display to exceed 50 and the red LED and beeper to stay on for longer than 15 seconds or a count of 20 beep beeps It is important to maintain stirring or swirling to detect the endpoint well Read the endpoint volume off of the syringe or burette This is your final volume SC 100A manual 5 Version 2 1c 11 Calculate the volume of titrant used V using the syringe or burette Starting volume minus final volume e g V 5 0 mL 3 5 mL 1 5 mL 12 The free SO gt content is calculated as 6
10. ey are somewhat fragile and will break if bent and straightened repeatedly Electrode care When done always rinse with DI water and let air dry There is no need to store the electrode in any kind of solution and the protective cap should not be replaced SC 100A manual 3 Version 2 1c Instrument Operation I Disconnect the electrode Turn on the instrument by pressing the POWER button briefly Note depressing the POWER button longer than two seconds at start up will cause the instrument to enter Test Mode see Appendix A The instrument will go through a power up sequence After a few seconds the display will show a value less than 50 usually 0 0 and the green Proceed LED should be on Optional Pre condition the SO Electrode All SO electrodes are pre conditioned at Vinmetrica before shipment Therefore normally you should skip this step If you think your electrode is not functioning properly you may try pre conditioning the electrode See Appendix C2 for more information on preconditioning the SO electrode Procedures Measuring Free SO by Titration l With the electrode disconnected turn on the instrument The display should show a value less than 20 usually 0 0 Now attach the SO electrode Fill the syringe by drawing up the SO gt Titrant the bottle with the blue label Figure 4 Expel bubbles and set the plunger on the syringe to a readable point preferably the 5 0 mL point This is your starting volume M
11. ions Press the POWER button briefly to move to the next section After the last section Test Mode restarts the first e Combinations of the yellow MODE LEDs are illuminated to indicate the section number as shown in the table below The Stop LED red is illuminated when an error is detected by the instrument The Proceed LED green is illuminated to indicate no error detected The green LED does not guarantee proper functioning it only indicates that no problem could be automatically detected The user should make careful observations to discern proper operation To exit Test Mode hold the POWER button down 5 10 seconds until the instrument shuts off If the device does not shut off after 10 seconds of holding down the button move to the next section by releasing then pressing again the POWER button briefly then try to exit again gt Serene 1 Version None The version number of the instrument software is displayed The instrument goes through a 2 Burn in None continuous burn in cycle exercising relay sound LEDs and display 3 pH probe or precision An uncalibrated pH level is shown in two voltage source Do alternating parts First the integer not exceed 0 5 V portion of pH level is shown 1 to 14 Next three decimal places are shown Readings above 14 000 are shown as Readings below 0 000 are shown as pH 4 SO SO probe or SO The SO current in nanoamperes 1s probe simulator e g
12. ls 10 Number None The display cycles through showing 14 CAL None only firmware Displays Cal tol then displays the tolerance v3 0 6 and later current value of the tolerance required to allow calibration Default is 0 5 pH units 1 e the displayed pH must be within 0 5 units of the target pH to allow calibration Pressing ENTER cycles between settings of 0 25 0 50 0 75 1 00 1 25 1 50 15 SOs None Disconnect SO gt Sets baseline value for SO2 mode Let Baseline electrode only firmware message scroll 5 sec then press ENTER v3 1 0 and later SC 100A manual 9 Version 2 1c Appendix B Sulfite Adjustments Using the Winemaker Magazine Sulfite Calculator Winemaker Magazine s Sulfite Calculator at http winemakermag com guide sulfite is an excellent tool for calculating how much sulfite should be added to your wine We will briefly go over the process here for some clarification l Select a Preferred method of Sulfite addition we recommend using a 10 solution of Potassium metabisulfite KMBS You can prepare this solution by weighing out 10g of KMBS and dissolving it in a FINAL volume of 100 mL DI water 2A Next choose the wine type red or white 3 Keep the Desired Molecular SO at 0 8 mg L and enter your wine s pH value hit your keyboard s Enter button after entering the value 4 After entering in your Desired molecular SO and your wine s pH the calculator will ask yo
13. mended amount of sulfite stir your wine thoroughly and take another SO measurement after waiting at least 30 minutes If the measurement matches the Desired level of free SO then you are done otherwise make incremental additions and repeated SO2 measurements until you reach your desired level SC 100A manual 10 Version 2 1c Appendix C Troubleshooting SQ Issues How stable are the reagents The SO reagents and the pH TA reagents are all warranted to last for 6 months We have found that in fact except the TA Titrant our reagents are stable for well over 12 months if stored tightly capped out of the heat and direct sunlight And of course these reagents will last much longer if not cross contaminated with each other How can I check the accuracy of my reagents It s rare that the SO reagents go bad but if you are concerned about it run the Ascorbic Acid Test method located in the FAQ section of the website to check your SO reagents If you are worried about your TA Titrant you can run the KHP test also located on the Vinmetrica website in the FAQ section under Manuals Tests and How To Videos at vinmetrica com FAQ I added the calculated amount of sulfite to my wine but the numbers are still low This is a common occurrence with several explanations any or all of which may be happening l Make sure you are using fresh sulfite powder Potassium metabisulfite degrades over time and that stuff you bought 2 year
14. minals then close the housing If desired you can prop the unit up using its folding stand 2 Low Battery Detection When the battery level is getting low the instrument shows a low battery icon on the upper left side of the display but continues to operate without impairment to any function Replace the batteries as soon as practicable When the battery level drops too far the instrument does not operate It rapidly flashes the low battery icon for 3 0 seconds beeps and shuts itself off 3 Auto Shut off The SC 100A shuts off after 30 minutes If this happens unexpectedly just press the POWER button to resume from where you were 4 When directed to do so see Procedures section below attach the SO electrode via the RCA phono plug connector protruding from the top Avoid any twisting motion just gently but firmly push the electrode plug straight onto the RCA connector to ensure proper function Figures 2 amp 3 Figure 2 Attach the Electrode Figure 3 Be sure the Electrode to the RCA connector on the attachment is secure on the RCA SC 100A connector 5 Remove any protective cover from the electrode tip some electrodes are shipped without one This cover is for shipping protection and need not be used routinely Put the electrode on its side or hang it from an electrode stand if you have one The SO electrode is sturdy with its plastic housing but do take care not to let things touch or strike the platinum wires th
15. nt and swirl in the usual way keeping SC 100A manual 11 Version 2 1c constant motion The instrument may or may not indicate STOP as above If it does not add a drop of the SO Titrant solution This should make the STOP condition occur If it doesn t you may have an electrode problem read in the next section below how to fix this Now add one drop of a concentrated sulfite solution 1 10 1s fine and verify that the STOP signal ends and the PROCEED light illuminates If this happens your electrode is probably OK as well 4 Finally you can check your SO reagents with the ascorbic acid vitamin C test located on our website vinmetrica com FAQ Preconditioning of the electrode Note as of October 2013 all SO2 electrodes are shipped pre conditioned so this procedure should not normally be needed Sometimes when the SQ elec trode is first shipped it can be shipped hot A hot electrode is one that is overly sensitive as indicated by high readings and STOP conditions when inserted into solutions that should be giving no signal like pure water or water plus reactant and acid with a drop of 10 KMBS added If your electrode shows this behavior it generally can be fixed by pre conditioning as follows l First turn the power on and press the MODE button until the instrument is in SO2 mode then press ENTER Attach the SO electrode to the SC 100 analyzer Put 20 mL of distilled water deionized water in a beaker and add half a bulb
16. on 2 1c Why Test for SO Testing for sulfite SO2 is crucially important for making sure your wine does not spoil by oxidation or from microbial growth Having the proper SO level in your wine gives you this protection By monitoring your SO levels you can make adjustments when needed especially before starting primary fermentation after malolactic fermentation has completed after racking or when ready to bottle To correctly adjust sulfite you need values for your current Free SO level and your wine s pH The SC 100A analyzer will help measure your current Free S02 You will need to obtain a value for your pH The key parameter in protecting your wine is Molecular SO2 which for most wines should be at 0 8 ppm mg L following primary fermentation This in turn depends on the Free SO it can also be referred to as unbound SO and the pH Overall you can reach your target Molecular SO by measuring and adjusting your free SO levels and considering your wine s particular pH See Table 1 Table 1 Free SO concentrations necessary to attain 0 8 mg L Molecular SO at a designated pH Free SO2 ppm 13 16 21 26 32 40 so 63 79 99 125 We recommend using a sulfite calculator for determining how much sulfite to add to your wines after taking a sulfite measurement with the SC 100A SO Analyzer Winemaker Magazine s Sulfite Calculator at http winemakermag com guide sulfite can walk you through the pro
17. s ago is probably bad now 2 Make sure that you stir your wine thoroughly when you add sulfite If you pour a 10 solution of KMBS into your wine it sinks like a battleship 3 A significant portion of the sulfite you added may have ended up bound particularly if your free SO was very low to begin with This bound SO does not show up when you measure free SOz and it is not protecting your wine You will need to add more sulfite until your free SO2 comes up to the right level Sometimes you must add two or even three times more sulfite than you first calculated I m getting strange results in SO mode how do I know if my instrument is working correctly For SO2 measurements with the SC 100A there are several quick tests you can do to make sure the instrument is not faulty L Be sure the battery is good per the manual s instructions P Remove the electrode to expose the RCA connector at the back of the instrument Turn on the instrument and select SO mode Short out the terminals on the connector using a paper clip or similar metal piece to touch the center pin of the connector to its outer metal sheath The device should indicate STOP with its red LED and buzzer or beeper If this does not happen there may be a problem with the instrument contact us for more information 3k Connect the electrode and put it in about 20 mL of distilled water add about 1 ml half a bulb squeeze of each of the acid solution and the reacta
18. squeeze of the acid solution half a bulb squeeze of the reactant solution and let the SO electrode sit in this solution 2 Then add one drop of 10 potassium metabisulfite solution KMBS to the beaker with the electrode in it Swirl gently The instrument LCD should now read 0 0 in units of nanoamps or close to it If it is reading significantly higher than 20 on the screen let the electrode sit in the solution for half an hour 3 After half an hour rinse the electrode with distilled water Put it in about 20 mL of distilled water Again add half a bulb squeeze of each of the acid solution and the reactant and swirl in the usual way keeping constant motion The instrument may or may not indicate STOP as above If it does not add a drop of the SO Titrant solution This should make the STOP condition occur If it doesn t you may have an electrode problem 4 Now add one drop of KMBS solution 1 10 is fine swirl and verify that the STOP signal ends and the PROCEED light illuminates If this is the case your electrode has been conditioned If the electrode is still hot and the PROCEED light does not illuminate let it sit in 20 mL of distilled water with a half bulb full of the acid solution for a few hours Now repeat the test from step 3 If it works great If not call us and we will try to troubleshoot or replace your electrode Technical assistance info vinmetrica com tel 760 494 0597 SC 100A manual 12 Version 2 1c
19. u to enter the Desired level of free SO The calculator already knows what this value is based on the data you entered and it should appear in the Notes section below the entry fields but the one fault of this app is that at least when we run it it won t tell you until you go through the calculation once So for now just enter 100 for the Desired level of free SO2 and proceed you will come back to this step in a moment to get the right answer see below 5 Now input the Current level of Free SO2 which you determined from measuring Free SO with the Vinmetrica SC 100 6 Finally enter the volume of wine to be corrected Choose liters or gallons we prefer liters because the answer is returned in mL The Amount of sulfite to be added should be in mL or fluid ounces of 10 sulfite solution or in grams if you use sulfite powder as your sulfite additive 7 Now press the blue Calculate button Ignore the answer below the Amount of sulfite to be added and look below to the Notes section You should now see the message 1 The recommended level of free SO for this type of wine molecular SO2 amp pH is your value mg L 8 Go back to step 4 and enter this value for the Desired level of free SO 9 Press Calculate again to get the correct amount of sulfite to add We recommend double checking your calculations Also be sure you are using fresh KMBS Once you have added the recom
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