Hardware User`s Manual Plethysmometer
Contents
1. SWITCH FUSE HOLDER Figure 1 Power inlet main switch and fuse holder FUSE REPLACEMENT OR VOLTAGE SETTING CHANGE In case of an over voltage or other incident in the AC net making it impossible to turn on the equipment or if the equipment voltage setting is incorrect check fuses according to the following procedure 1 Remove power cord from the power inlet Plethysmometer A Harvard Apparatus Panlab H 2 Open fuse holder by pulling the flange with a regular screwdriver Figure 2 Open fuse holder door 3 Extract fuse holder using the screwdriver AE SNE ee A L Figure 3 Extract fuse holder 4 Replace fuses if necessary Insert fuses in the fuse holder in the correct position CORRECT INCORRECT Figure 4 Fuses position 5 Insert the fuse holder again positioning it according to the voltage in the AC net 115V POSITON 230V POSITION Figure 5 Fuse holder position 6 Ifthe fuses blow again unplug the equipment and contact technical service N For electrical safety reasons never open the equipment The power WARNING Supply has dangerous voltage levels Plethysmometer Panlab H 5 TABLE OF CONTENTS 1 SYMBOLS TABLE 2 GOOD LABORATORY PRACTICE 3 UNPACKING AND EQUIPMENT INSTALATION 4 MAINTENANCE 5 TABLE OF CONTENTS 6 INTRODUCTION 7 EQUIPMENT DESCRIPTION 7 35 SENSOR UNIT 7 2 CONTROL UNIT FRONT PANEL 7 3 CONTROL UNIT REAR PANEL 8 WORKING WIT2. 17 Panlab H Nim 10 In order to clean the flasks and the container you should use a soapy solution and cleanning them with distilled water Afterwards they should be dried with a dry cloth WARNING Never use alcohol or alcohol based detergents to clean Perspex parts if used grooves will appear in the transparent plastic This damage is not covered by the warranty 8 7 ERROR SIGNALS Depending on the operating mode the DISPLAY can show 2 information messages 8 7 1 ERRC CAUSE Calibration is not permitted because the increase in the signal picked up by the electrode is insufficient due to one of the following causes Check e The level of salinity of the solution is low check with the button e The volume of the volumetric flask used is too big e Thetransducer is in the wrong position SOLUTION e Correct the salinity increase salinity e Usea smaller volumetric flask e Place the transducer in the right position 8 7 2 ERRS CAUSE The level of salinity of the solution is outside tolerance The correct margins are between 40 and 160 Levels below 40 indicate that the solution must be more concentrated add NaCl Levels above 160 indicate that the solution must be diluted add distilled water SOLUTION Correct the concentration of the solution Plethysmometer 18 Harvar Panlab HA Hever 9 WORKING WITH THE SEDACOM SOFTWARE The purchase of the Sedacom software option is needed for tr
3. DISPLAY value and send data to the PC e POWER The mains lead is connected here It also contains the main switch and the fuse holder e CALIBRATION ml This switch is used to select the volume of the volumetric flask that we will use For 1 ml flask select position 1 For 3 ml flask select position 3 and for 5 ml flask select position 5 Each type of volumetric flask has to be calibrated with its own known volume Plethysmometer 11 Panlab H Ark i 8 WORKING WITH THE EQUIPMENT 8 1 EQUIPMENT CONNECTION The system connection is shown in the following figure Figure 11 Equipment connection Cables and connections are listed in the following table FROM CABLE PC serial port LE7500 RS 232 RS232 Cable LE 7500 Pedal Jack 6 35 mm LE7500 Probe Platinum electrode Jack 3 5 mm Silicone pipe Hydraulic connection Plethysmometer 12 Panlab ed 8 2 PREPARING THE SOLUTION The solution will have a 0 2 weight volume concentration of NaCl In other words 2 g of NaCl per litre of NON deionised distilled water Add 15 drops of TRITON per litre of solution this is to keep the water from forming a concave meniscus on the flask walls through surface tension 8 3 CHECK SOLUTION SALINITY 1 Connect the equipment as shown in Figure 11 2 Fillthe CONTAINER with the salt solution prepared as described in section 8 2 3 Openthe valve to fill the volumetric and sensor flasks You must fill bo
4. elektronische Ger te d rfen nicht mit dem Hausm ll entsorgt werden Der Verbraucher ist gesetzlich verpflichtet elektrische und elektronische Ger te am Ende ihrer Lebensdauer an den daf r eingerichteten ffentlichen Sammelstellen oder an die Verkaufstelle zur ckzugeben Einzelheiten dazu regelt das jeweilige Landesrecht Das Symbol auf dem Produkt der Gebrauchsanleitung oder der Verpackung weist auf diese Bestimmungen hin Mit der Wiederverwertung der stofflichen Verwertung oder anderer Formen der Verwertung von Altger ten leisten Sie einen wichtigen Beitrag zum Schutz unserer Umwelt H P Informazioni per protezione ambientale Dopo l implementazione della Direttiva Europea 2002 96 EU nel sistema legale nazionale ci sono le seguenti applicazioni dispositivi elettrici ed elettronici non devono essere considerati rifiuti domestici consumatori sono obbligati dalla legge a restituire dispositivi elettrici ed elettronici alla fine della loro vita utile ai punti di raccolta collerici preposti per questo scopo o nei punti vendita Dettagli di quanto riportato sono definiti dalle leggi nazionali di ogni stato Questo simbolo sul prodotto sul manuale d istruzioni o sull imballo indicano che questo prodotto soggetto a queste regole Dal riciclo e re utilizzo del material o altre forme di utilizzo di dispositivi obsoleti voi renderete un importante contributo alla protezione dell ambiente P Nota em Protec o Ambien
5. legalmente obligado a llevar los aparatos el ctricos y electr nicos as como pilas y bater as al final de su vida til a los puntos de recogida municipales o devolverlos al lugar donde los adquiri Los detalles quedaran definidos por la ley de cada pa s El s mbolo en el producto en las instrucciones de uso o en el embalaje hace referencia a ello Gracias al reciclaje a la reutilizaci n de materiales i a otras formas de reciclaje de e usados usted contribuir de forma importante a la protecci n de nuestro medio ambiente P H Remarques concernant la protection de l environnement Conform ment la directive europ enne 2002 96 CE et afin d atteindre un certain nombre d objectifs en mati re de protection de l environnement les r gles suivantes doivent tre appliqu es Elles concernent les d chets d quipement lectriques et lectroniques Le pictogramme picto pr sent sur le produit son manuel d utilisation ou son emballage indique que le produit est soumis cette r glementation Le consommateur doit retourner le produit usager aux points de collecte pr vus cet effet peut aussi le remettre un revendeur En permettant enfin le recyclage des produits le consommateur contribuera la protection de notre environnement C est un acte cologique D Hinweis zum Umweltschutz Ab dem Zeitpunkt der Umsetzung der europ ischen Richtlinie 2002 96 EU in nationales Recht gilt folgendes Elektrische und
6. E Ww 2 GOOD LABORATORY PRACTICE Check all units periodically and after periods of storage to ensure they are still fit for purpose Investigate all failures which may indicate a need for service or repair Good laboratory practice recommends that the unit be periodically serviced to ensure the unit is suitable for purpose You must follow preventive maintenance instructions In case equipment has to be serviced you can arrange this through your distributor Prior to Inspection Servicing Repair or Return of Laboratory Equipment the unit must be cleaned and decontaminated Decontamination prior to equipment disposal In use this product may have been in contact with bio hazardous materials and might therefore carry infectious material Before disposal the unit and accessories should all be thoroughly decontaminated according to your local environmental safety laws Plethysmometer Harvar Panlab H Harvard 3 UNPACKING AND EQUIPMENT INSTALATION WARNING Failure to follow the instructions in this section may cause equipment faults or injury to the user A No special equipment is required for lifting but you should consult your local regulations for safe handling and lifting of the equipment B Inspect the instrument for any signs of damage caused during transit If any damage is discovered do not use the instrument and report the problem to your supplier C Ensure all transport locks are removed before use The original pa
7. H Harvard Apparatus This table features instructions to solve the most frequent problems PROBLEM SOLUTION The equipment does not start up When CALIBRATION button is pressed to calibrate the device the display shows the error message ERRC When CHECK SOLUTION button is pressed to test the saline solution the display shows the error message ERRS The measurements are not sent to the Sedacom program by pressing the foot switch Plethysmometer Ensure that the voltage of mains is the same as that selected in the fuse holder Check the condition of the fuses The level of salinity of the solution is low check with the CHECK SOLUTION button Add salt to the solution The volume of the volumetric flask used is too big You must use the proper calibrated volume The transducer is in the wrong position Place it correctly If the displayed value is below 40 the saline has little salt you should increase the salt concentration If the displayed value is greater than 160 the saline solution is too much salty you must reduce the concentration of salt Check that the RS232 cable is connected between the computer and the equipment Check that the foot switch is connected to the equipment Check the configuration of serial port and device in the program Sedacom If you are using a USB to RS232 adapter check the installation of the drivers on the computer 20 Panlab H 11 PREVENTIVE MAINTENA
8. H THE EQUIPMENT 8 1 EQUIPMENT CONNECTION 8 2 PREPARING THE SOLUTION 8 3 CHECK SOLUTION SALINITY 8 4 EQUIPMENT CALIBRATION 8 5 TAKING MEASUREMENTS 8 6 EMPTYING AND CLEANING THE SENSOR UNIT 8 7 ERROR SIGNALS 8 7 1 ERRC 0 7 2 ERRS 9 WORKING WITH THE SEDACOM SOFTWARE 10 TROUBLESHOOTING 11 PREVENTIVE MAINTENANCE 12 TECHNICAL SPECIFICATIONS Plethysmometer Harvard Apparatus 10 11 12 12 13 13 14 16 17 18 18 18 19 20 21 22 Panlab HA jen 6 INTRODUCTION The LE 7500 Digital Plethysmometer is an instrument used to determine variations in the volume of rodent limbs It measures the variation in fluid level when the limb is inserted into a tank The introduction of a tissue or any object into the container changes the level of fluid and the conductivity between two platinum electrodes previously introduced into the container This change is expressed in millilitres with a resolution of 0 01 ml Figure 6 LE 7500 Digital Plethysmometer Plethysmometer Panlab H Ark i 7 EQUIPMENT DESCRIPTION 7 1 SENSOR UNIT CONTAINER T gt og ELECTRODE SENSOR FLASK VOLUMETRIC FLASK VALVE Figure 7 Sensor unit e CONTAINER Container which holds the maximum volume of solution designed to fill the other two for this reason it is placed in a higher position as the other containers are filled by gravity e VOLUMETRIC FLASK Container into which the
9. Hardware User s Manual Plethysmometer References L E7500 76 0220 LE7504 76 0221 LE7503 76 0223 LE7505 76 0222 Version V29 10 2014 T Panlab s l u International Calls 34 934 750 697 Pa n a b H AN Harvard C Energia 112 Domestic Call 934 190 709 ew D 08940 Cornella de Ll Barcelona Fax 34 934 750 699 Spain Info Qpanlab com www panlab com Limitation of Liability PANLAB does not accept responsibility under any circumstances for any harm or damage caused directly or indirectly by the incorrect interpretation of what is expressed in the pages of this manual Some symbols may have more than one interpretation by professionals unaccustomed to their usage PANLAB reserves the right to modify in part or in total the contents of this document without notice Panlab H Ni 1 SYMBOLS TABLE Recognising the symbols used in the manual will help to understand their meaning DESCRIPTION SYMBOL Warning about operations that must not be done because they can damage the equipment Warning about operations that must be done otherwise the user can be N exposed to a hazard Protection terminal ground connection D Warning about a hot surface which temperature may exceed 65 C N Warning about a metal surface that can supply electrical shock when it s A touched Decontamination of equipments prior to disposal at the end of their operative life Waste Electrical and Electronic Equipment Directive WEE
10. NCE WHEN EXPERIMENT NECESSARY CLEAN THE FLASKS CLEAN PLATYNUM ELECTRODE CALIBRATE THE EQUIPMENT CHECK CABLES CONNECTION PREPARE NEW SALTY SOLUTION Plethysmometer Harvard Apparatus 21 Panlab H 12 TECHNICAL SPECIFICATIONS Harvard Apparatus POWER SUPPLY Input Voltage Frequency Fuses Maximum Power Conducted noise 115 230 VAC 50 60 Hz 2 fuses 5mm 20mm 100MA 250V 12W EN55022 CISPR22 CISPR16 class B BEHAVIOURARL CONDITIONS Working temperature Working relative humidity Storage temperature 10 C a 40 C 096 a 8596 RH without condensation 09C a 50 C without condensation COMMUNICATIONS Interface RS232C Connector Connector Delta 9 female DIMENSIONS Width x Height x Depth 232mm x 114mm x 297mm Weight 3 58 kg Plethysmometer 22 Panlab H Mac DECLARACION DE CONFORMIDAD DECLARATION OF CONFORMITY DECLARATION DE CONFORMITE Nombre del fabricante Panlab s l u Manufacturer s name www panlab com Nom du fabricant info panlab com Direccion del fabricante Energia 112 Manufacturer s address 08940 Cornell de Llobregat Adresse du fabricant Barcelona SPAIN Declara bajo su responsabilidad que el producto PLETISMOMETRO DIGITAL Declares under his responsibility that the product D clare sous sa responsabilit que le produit Marca Brand Marque PANLAB Modelo Model Modele LE 7500 Cumple los requisitos esenciales establecidos
11. ansferring the data to a computer please contact your local sales delegate for more information The Sedacom software reference is composed a USB Flash key containing the software Installer License for use and Sedacom User s Manual Follow the next instructions e Please refer to the Sedacom User s Manual for instructions about how to install and use the software with the present device e A serial port RS232 communications cable provided with the present device is needed for the connection between the present device and the computer in which the Sedacom software is installed Please refer to the present User s Manual for instructions about how to connect this cable to the device chapter 9 1 e Ifthe computer doesn t have any serial port the RS232 USB adapter is needed ref CONRS232USB contact your local sales delegate for more information WARNING the RS232 communication cable provided with the device is used for any connection of the device with an associated software Sedacom etc When the device is used without software in first instance this cable needs to be preserved and kept in secure place in case the need of using the system with a software arises in the future In this last case if the user lost the cable a new one should be purchased to his local sales delegate ref CONRS232 The warranty time of this cable is the same than the warranty time of the device Plethysmometer 19 10 TROUBLESHOOTING Panlab
12. cking has been especially designed to protect the instrument during transportation It is therefore recommended to keep the original carton with its foam parts and accessories box for re use in case of future shipments Warranty claims are void if improper packing results in damage during transport D Place the equipment on a flat surface and leave at least 10 cm of free space between the rear panel of the device and the wall Never place the equipment in zones with vibration or direct sunlight E Once the equipment is installed in the final place the main power switch must be easily accessible F Only use power cords that have been supplied with the equipment In case that you have to replace them the spare ones must have the same specs that the original ones G A Make sure that the AC voltage in the electrical network is the same as the voltage selected in the equipment Never connect the equipment to a power outlet with voltage outside these limits AN For electrical safety reasons you only can connect equipment to power outlets provided with earth connections This equipment can be used in installations with category Il over voltage according to the General Safety Rules WARNING The manufacturer accepts no responsibility for improper use of the equipment or the consequences of use other than that for which it has been designed Plethysmometer Panlab H Hever PC Control Some of these instruments are designed to be contr
13. ge The correct margins are between 40 and 160 Levels below 40 indicate that the solution must be more concentrated more NaCl Levels above 160 indicate that the solution must be diluted less NaCl The ideal value is 100 To measure salinity fill the VOLUMETRIC FLASK with liquid up to the reference mark and hold down the button If it is pressed briefly the value will be displayed for 1 second If the solution is incorrect the DISPLAY will also show ErrS e CALIBRATION This makes possible to indicate to the instrument that the increase in volume produced by a known volume 3 00 ml corresponds to 3 00 ml optionally it can be calibrated to 1 00 ml or 5 00 ml using special FLASKS and calibres and thus adjust its internal gain This prevents errors caused by changes in salinity and changes in the initial fluid level Plethysmometer 10 Panlab Md 7 3 CONTROL UNIT REAR PANEL CALIBRATION ml LE 7500 J Y Calibration j WY RS 232 DOOOO E Made n Spain 11p 220 V d Ad 50 60 Hz RS 232 PROBE PEDAL POWER Figure 10 Rear panel e RS 232 9 pin serial connector that allows the user to connect the CONTROL UNIT to an external device PC to process and work with the data obtained through the Sedacom software not included should be purchased separately e PROBE Connector to which the lead of the PROBE of the SENSOR UNIT is attached e PEDAL The PEDAL is connected to the CONTROL UNIT to set the
14. ncorporate the CE and FCC marking C En cons quence ce produit peut incorporer le marquage CE et FCC En representaci n del fabricante Manufacturer s representative En repr sentation du fabricant Carme Canal s General Manager WENT Panlab s l u a division of Harvard BioScience Cornell de Llobregat Spain 30 06 2014 Plethysmometer 23 Harvard Apparatus Panlab H Note on environmental protection After the implementation of the European Directive 2002 96 EU in the national legal system the following applies Electrical and electronic devices may not be disposed of with domestic waste Consumers are obliged by law to return electrical and electronic devices at the end of their service lives to the public collecting points set up for this purpose or point of sale Details to this are defined by the national law of the respective country This symbol on the product the instruction manual or the package indicates that a product is subject to these regulations By recycling reusing the materials or other forms of utilising old devices you are making an important contribution to protecting our environment tat H Nota sobre la protecci n medioambiental Despu s de la puesta en marcha de la directiva Europea 2002 96 EU en el sistema legislativo nacional Se aplicara lo siguiente Los aparatos el ctricos y electr nicos as como pilas y bater as no se deben tirar ala basura dom stica El usuario est
15. o the VOLUMETRIC FLASK 5 Press the PEDAL if you wish to save the reading or send it to the PC The Hold reading on the DISPLAY will be set and the 9 led is turned on Hold 6 Press the PEDAL again to release the DISPLAY and the 9 led will be turned off 7 Return to step 4 to take a new measurement WARNING e If the level of liquid has changed the level should be reset to zero using the Zero El button Plethysmometer 16 Panlab GA Harvard Apparatus e The unit can be recalibrated after a certain time to achieve greater precision 8 6 EMPTYING AND CLEANING THE SENSOR UNIT 1 Switch off the CONTROL UNIT before emptying the SENSOR UNIT 2 Remove the CONTAINER and place it at a level below the flasks SCREW Figure 17 Container 3 Turnthe valve to empty the flasks into the CONTAINER 4 Closethe gate valve 5 Removethe valve from the flasks 6 Empty the CONTAINER through the valve to the sewage We do not advise to keep the used solution 7 Once the flasks and the container are empty remove the platinum electrode and clean it carefully with distilled water WARNING Do not touch the platinum electrode Figure 18 Platinum Electrode 8 Allow the electrode to air dry never dry it with a cloth since the electrode is deformed easily and would lose its linearity affecting the measurements done 9 Oncedry place the electrode in its starting position in the SENSOR FLASK Plethysmometer
16. olled from a PC To preserve the integrity of the equipment it is essential that the attached PC itself conforms to basic safety and EMC standards and is set up in accordance with the manufacturers instructions If in doubt consult the N information that came with your PC In common with all computer operation the following safety precautions are advised WARNING To reduce the chance of eye strain set up the PC display with the correct viewing position free from glare and with appropriate brightness and contrast settings e To reduce the chance of physical strain set up the PC display keyboard and mouse with correct ergonomic positioning according to your local safety guidelines Plethysmometer Panlab ed 4 MAINTENANCE WARNING Failure to follow the instructions in this section may cause equipment fault e PRESS KEYS SOFTLY Lightly pressing the keys is sufficient to activate them e Equipments do not require being disinfected but cleaned for removing urine faeces and odour To do so we recommend using a wet cloth or paper with soap which has no strong odour NEVER USE ABRASIVE PRODUCTS OR DISSOLVENTS e NEVER pour water or liquids on the equipment e Once you have finished using the equipment turn it off with the main switch Clean and check the equipment so that it is in optimal condition for its next use e Theuser is only authorised to replace fuses with the specified type when necessary OPENING FLANGE
17. por la Uni n Europea en las directivas siguientes Fulfils the essential requirements established by The European Union in the following directives Remplit les exigences essentielles tablies pour l Union Europ enne selon les directives suivantes 2006 95 EC Directiva de baja tensi n Low Voltage Basse tensi n 2004 108 EC Directiva EMC EMC Directive Directive CEM 2012 19 EU La Directiva de Residuos de Aparatos El ctricos y Electr nicos WEEE The Waste Electrical and Electronic Equipment Directive WEEE Les d chets d quipements lectriques et lectroniques WEEE 2011 65 EU Restricci n de ciertas Sustancias Peligrosas en aparatos el ctricos y electr nicos ROHS Restriction of the use of certain Hazardous Substances in electrical and electronic equipment ROHS Restriction de l utilisation de certaines substances dangereuses dans les quipements lectriques et lectroniques ROHS 2006 42 EC Directiva mec nica Machinery directive Directive m canique Para su evaluaci n se han aplicado las normas armonizadas siguientes For its evaluation the following harmonized standards were applied Pour son valuation nous avons appliqu les normes harmonis es suivantes Seguridad Safety S curit EN61010 1 2011 EMC EN61326 1 2012 Class B FCC FCCA47CFR 15B Class B Safety of machinery EN ISO 12100 2010 En consecuencia este producto puede incorporar el marcado CE y FCC Consequently this product can i
18. t 15 minutes thus the platinum electrode will be impregnated and the equipment will not have measurements drifts The equipment s display will show a value Figure 15 Calibrated volume dip Calibration 8 Press the E button to tell the equipment the calibrated volume measurement then the display will show the value of the volume for example when working with the flask of aml the display will show 1 00 Hold 9 Press the foot switch to set the measurement then the 9 led will light up indicating that the calibrated volume can be removed 10 Dry the calibrated volume Plethysmometer 15 Panlab H Aden Hold 11 Press the foot switch again the equipment will do a zero and the 9 led will turn off 12 Dip the calibrated volume again and the measurement showed on the display should be correct if the value deviates from the expected one press the Calibration ES and return to step 9 8 5 TAKING MEASUREMENTS Hold 1 Power up the CONTROL UNIT LE 7500 The LED will come on and after a few seconds the DISPLAY will show o oo 2 Turn the valve for the solution to enter the VOLUMETRIC FLASK until it reaches the mark on the VOLUMETRIC FLASK Press the ZERO buttons to indicate the starting point o millilitres of volume Figure 16 Valve 3 Calibrate the instrument see chapter 8 4 4 When the DISPLAY is at o oo insert the limb the volume of which is to be determined int
19. tal Ap s a implementa o da directiva comunit ria 2002 96 EU no sistema legal nacional o seguinte aplica se Todos os aparelhos el ctricos e electr nicos n o podem ser despejados juntamente com o lixo dom stico Consumidores est o obrigados por lei a colocar os aparelhos el ctricos e electr nicos sem uso em locais p blicos especficos para este efeito ou no ponto de venda Os detalhes para este processo s o definidos por lei pelos respectivos pa ses Este simbolo no produto o manual de instru es ou a embalagem indicam que o produto est sujeito a estes regulamentos Reciclando reutilizando os materiais dos seus velhos aparelhos esta a fazer uma enorme contribuig o para a protecc o do ambiente PG Plethysmometer 24
20. th flasks until the saline solution reaches the mark Figure 12 Fill the flasks up to the mark level 4 Turn on the equipment and wait a few seconds for it to start up Check 5 Press the button in order to check solution salinity Correct values are between 40 and 160 if the salinity is out of this range the display will show for few seconds the error ERRC see chapter 8 7 Plethysmometer 13 Panlab HA tove 8 4 EQUIPMENT CALIBRATION 1 Connectthe equipment as shown in Figure 11 2 Set the rear panel CALIBRATION switch in the nu position that matches with the volumetric flask we will work with That is if we work with aml flask we Calibration Sml will set the selector to 1ml and so respectively for 5ml cases of 3ml and 5ml a b C Figure 13 Flasks and calibrated volumes a LE7504 1ml b LE7503 3ml and c LE7505 5ml 3 Fillthe CONTAINER with the salt solution prepared as described in section 8 2 4 Open the valve to fill the volumetric and sensor flasks You must fill both flasks until the saline solution reaches the mark Plethysmometer 14 Panlab H Havers E B VALVE Figure 14 Fill the flasks up to the mark level 5 Turn on the equipment and wait a few seconds for it to start up Zero 6 If the display does not show o oo press the button so that the equipment balances the solution level 7 Dip the calibrated volume in the volumetric flask and wai
21. tissues are placed It is connected to the SENSOR FLASK It has a mark at the top to help determine the maximum level of solution e VALVE This controls the amount of liquid entering the VOLUMETRIC FLASK and SENSOR FLASK e SENSOR FLASK This is where the displaced volume of solution is calculated It holds the two platinum electrodes that record the changes in conductivity which indicate the exact volume of the tissues PLATINUM ELECTRODE Figure 8 Platinum Electrode Plethysmometer Panlab H Nim Hold HARVARD Parjlab APPARATUS HOLDLED DISPLAY ZERO CHECK SOLUTION CALIBRATION 7 2 CONTROL UNIT FRONT PANEL DIGITAL PLETHYSMOMETER LE 7500 ml Check Solution Figure 9 Front Panel e HOLD This LED indicates that the information on the DISPLAY is set Press the PEDAL to block unblock it Moreover on pressing the PEDAL the information is sent to the PC via the RS 232 serial line connected to the rear panel e DISPLAY This indicates the value of the volume measured in ml and the solution s salinity level as a percentage e ZERO On pressing and releasing this button the DISPLAY is reset and the initial level of liquid is set on the apparatus The device performs this function automatically on removing the measured volume from the VOLUMETRIC FLASK e CHECK SOLUTION This is to check that the solution is correct The device measures the level of salinity of the solution and expresses it as a percenta
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