User Manual 202CM
Contents
1. E RENE 3 SAN SOW EUIS T m m S 5 EMC EMI m 6 APP OVAS sce ace circ eee cc ee 6 Certificate of ConfOFTllby ussoesesbaexekkts venir a Md RR RbRY DNE KERN EE UN CER EC M wi ERA n ERR D A DERI ICI E ROLE EN a cx ER Y mu E ERE R FR I dr EN n UR wir GR E RR 7 POU A EES E E E E E E E A E E 8 Unpacking the Culture Myograph System ssnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn E rr i 9 Chapter 1 System Overview B RO Er RR D c EU nnmnnn 10 T E oa E A E DAEA AE E EA E AAA E 10 DVE MEO O T E T E EEEO A E A M 11 1 3 Culture My graph Heat Controlle a aa Aa aaa RN UU 11 Chapter 2 Setting UD ec anaa Aaria ENANA Ea Sanao ENNAN EKNE EEan aAa 12 2 1 The Complete Culture Myograph System 202 1 Octa va blade 12 2 2 Setting up complete Culture Myograph System 202 18 2 UNT TU 18 Chapter 3 DMT 202CM Control2. Micropositioner for longitudinal regulation of right glass cannula d Vertical regulation plate for e left glass cannula D O ring locks fixing the left glass cannula Fixation and longitudinal e regulation plate for right glass cannula Screw for horizontal regula tion of both glass cannulas NAP URP e Su perfusion outlet Left glass cannula Tip outer diameter 125um Qe nitens sees e Right glass cannula Tip outer diameter 125 Myograph chamber access hole for left glass cannula Myograph chamber access hole for right glass cannula 4 e Culture myograph unit chamber assembly mark 00900000000000000000000000000000000000000 Superfusion inlet e Figure 1 1 Culture Myograph Chamber Unit 10 CULTURE MYOGRAPH SYSTEM MODEL 202CM USER MANUAL 1 2 DMT Microscope p e 4 p p p esso Red Light intensity i Pp Lever to fasten culture myograph regulation plug A unit Fig 1 1 Knob for connection of Infrared light scource e Zeiss Achromat 10X X Y Z level regulation of temperature probe 0 25 objective objective focus Figure 1 2 DMT Microscope 1 3 Culture Myograph Heat Controller Power ON LED e Connection to the Culture Myograph Units 110 240V AC 50 60HZ 1 6 AT Power Inlet USB Port for Connection to Computer ON OFF Switch Figure 1 3 Heat Controller CHAPTER 1 11 CHA
3. Figure 4 5 How to adjust the position and focus of the microscope in the X Y Z directions The DMT VAS obtains an image by measuring the differences in light intensity passing through the walls of a vessel segment mounted in the culture chamber Traditionally a white light source is sufficient for such a purpose but has one major disadvan tage A white light source makes the data acquisition and analysis very sensitive to changes in white light intensity from the sur roundings such as ambient light and sunlight To avoid the surroundings influencing the data acquisition and analysis the DMT microscope has a built in infrared light source 4 3 Pressure Regulator The Pressure Regulator is connected to the culture myograph system to generate a pressure on the mounted vessel from the perfusion buffer The pressure is adjusted using the knob and air release valve shown in figure 4 6 below Figure 4 6 Pressure Regulator 24 CULTURE MYOGRAPH SYSTEM MODEL 202CM USER MANUAL 4 4 Culture Myograph Maintenance The Culture Myograph System Model 202 is a very delicate and sophisticated piece of research equipment In order to keep it working at its best DMT recommend that the following sections are read carefully and that the instructions are followed at all times DMT STRONGLY RECOMMENDS THAT THE MYOGRAPH CHAMBER AND SURROUNDINGS BE CLEANED AFTER EACH EXPERI MENT After a normal experiment use the following procedure to clean the myog
4. either clockwise or counter clockwise direction to adjust the horizontal alignment of the glass cannulas Horizontal Adjustment Screw Loosen this screw and carefully turn clockwise or counter clockwise Observe how the pippettes move in horisontal plane Figure 4 2 Horizontal adjustment of glass pipettes Vertical Adjustment Vertical adjustment is performed as illustrated in figure 4 1 on previous page Gently turn the black dish underneath the left glass cannula clockwise or counter clockwise to adjust the vertical alignment of the glass cannulas Longitudinal Adjustment Longitudinal adjustment of the glass cannulas is performed using the micro positioner on the right side of the culture myograph unit CHAPTER 4 21 4 1 2 Removal and Mounting of Glass Cannulas and Chamber Replacement of glass cannulas and demounting of the culture myograph chamber for sterilisation are common routines in cul ture myograph experiments The procedure is illustrated in figure 4 3 below Fixation screw O rings Lock screw Figure 4 3 How to free the glass cannulas from the culture myograph unit Removal of Left Glass Cannula Carefully remove the two O rings marked by the two arrows to free the glass cannula from the culture myograph unit Then care fully unscrew the locknut and remove both locknut and glass cannula Removal of right Glass Cannula Gently remove the little screw marked by the arrow to free the glass cann
5. will continue to load the IC Capture software Click on Next when the IC Capture Setup Wizard screen appears You will need to agree to the License Agreement to proceed On the next screen enter your User Information and License Key which is found on the front of the CD sleeve Click on Next The Installation program will ask you where you want to install the software on your hard drive Once you have deter mined an appropriate drive and folder click Next The IC Capture software will be installed Click Finish 3 Turning Off the Auto Shutter Function of the Camera A Launch the IC Capture 2 1 software When you first launch the software it will ask you to recognize a camera The cam era will be listed as DMx 41AU02 AS On your keyboard press CTRL 5 A tool bar should appear with Gain and Exposure Unclick the auto functions This just turned off the auto shutter func tions of the camera Close the IC Capture software 4 Installing the DMT Vessel Acquisition Suite DMT VAS software A B At this point the camera should be installed and functional Install the DMT VAS software by double clicking on the dmt O9Mar10 exe file that came on your or that you down loaded This will automatically install the software to your C drive Open your C drive and you will find a stand alone folder dmt Open the dmt folder Open the current folder Find the application file DMT VAS Double clicking on t
6. 1 Residential commercial and light industry With reference to regulations in the following directives 3 23 EEC 89 336 EEC CERTIFICATE OF CONFORMITY 7 ABOUT THIS MANUAL This manual contains a complete list of procedures describing how to install maintain and using the Culture Myograph System Model 202CM Chapter 1 provides a comprehensive view of the construction and basic features of the complete Culture Myograph System Chapter 2 describes step by step how to set up a complete 202CM Culture Myograph System including all various accessories Chapter 3 describes Control of Temperature and Light Intensity Chapter 4 is a complete manual to the Culture Myograph System The chapter describes in detail how to use the DMT micro scope how to use and adjust the culture myograph chamber and finally instructions for the daily maintenance of the Culture Myograph System Appendixes contain additional information about fuse replacement and system specifications 8 CULTURE MYOGRAPH SYSTEM MODEL 202CM USER MANUAL UNPACKING THE CULTURE MYOGRAPH SYSTEM Please take a few minutes to carefully inspect your new Culture Myograph System for any damage which may have occurred dur ing handling and shipping If you suspect any kind of damage please contact DMT immediately and the matter will be pursued as soon as possible If the packing material appears damaged please retain it until a possible claim has been settled We recommend that you sto
7. A Tel 1 770 612 8014 Fax 1 678 302 7013 www dmt usa com sales dmt usa com support dmt usa com
8. DANISH MYO TECHNOL Ost CULTURE MYOGRAPH SYSTEM MODEL 202CM User Manual Version 1 6 2 CULTURE MYOGRAPH SYSTEM MODEL 202CM USER MANUAL CULTURE MYOGRAPH SYSTEM MODEL 202CM TRADEMARKS Pentium is a registered trademark of the Intel Corporation Windows Windows 95 Windows 98 Windows ME Windows NT Windows 2000 and Windows XP are registered trademarks of Microsoft Corporation VediView software is a trademark of Phot onics Engineering Euresys Picolo Frame grabber All other trademarks are the properties of their respective owners DMT A S reserves the right to alter specifications as required This document was as far as possible accurate at the time of printing Changes may have been made to the software and hardware described since then New information may be supplied separately This documentation is provided with the DMT Culture Myograph System Model 202CM Document Number 202CM 001A All rights reserved No part of this manual may be reproduced or transmitted in any form or by any means without the written permission of Danish Myo Technology A S Every attempt is made to ensure accurate information misprints construction and specification changes can occur Danish Myo Technology A S reserves the right to alter change content as required and without any notice Copyright Danish Myo Technology A S DANISA M170 TECHNOL OGS TRADEMARKS 3 CONTENTS TE ACN ccs sac aes A es sc Se
9. PTER 2 SETTING UP 2 1 The Complete Culture Myograph System 202CM Pewee eee m eee eee ee M m o m m m m wee ee c m ooo mop DC Adapter 90 240V Rear Panel Pressure Regulator Front Panel o o o O Heat Controller Front Panel Heat Controller Rear USB Connections PC Data Acquisition Panel and Analysis Software Optional Oxygen Superfusion Supply Buffer Outlet Superfusion buffer T Inlet Superfusion buffer TM Inflow Perfusion buffer Electrical connections Figure 2 1 Setting up step by step NOTE IF YOU HAVE PURCHASED A COMPUTER FROM DMT ALONG WITH YOUR CULTURE MYOGRAH 202CM SYSTEM THE DMT VAS HAS ALREADY BEEN INSTALLED ALONG WITH DRIVERS FOLLOW THE PROCEDURES IN 2 2 SETTING UP THE COMPLETE CULTURE MYOGRAPH SYSTEM 202CM ON PAGE 13 TO SET UP THE CULTURE MYOGRAPH SYSTEM IF YOU HAVE NOT PURCHASED A COMPUTER FROM DMT PLEASE FOLLOW THE PROCEDURES IN 2 3 INSTALLATION OF DMT VAS ON PAGE 13 TO INSTALL THE DMT VAS ON YOUR OWN COMPUTER 12 CULTURE MYOGRAPH SYSTEM MODEL 202CM USER MANUAL 2 2 Setting up complete Culture Myograph System 202CM This section describes how to connect the cables in the culture myograph system as illustrated in figure 2 1 on previpos page Before connecting ny of the myograph equipment ensure that the frame grabber card is installed into the PCI slot o
10. Program 18 3 1 Control of Temperature and NI 18 Chapter 4 Culture Myograph 20 Myo raD UN TT 20 Zu Tue DME MITO CON oriee E 24 E 24 4A Culture Myorraph PTT 25 Appendix 1 Fuse replacement ERE kan ER D UN Ru EROR ER REDE RGB ERU RR RE ER CE D RR UA nnmnnn nnmnnn nnmnnn 26 Appendix 2 System Specifications Lire ecce eeee ecu seen nena naa a use a aaa a IIIa Ras SII D RR RR S SR RRRR OR SNR R RR I Sus s Or a rasa nnna 27 4 CULTURE MYOGRAPH SYSTEM MODEL 202 USER MANUAL SAFETY The Culture Myograph System has been designed for use only in te
11. a free USB port on your computer If your system is a 110P then your camera needs to be installed on the microscope and the USB cable that came with the system needs to be con nected to the camera and the camera connected to the computer through a free USB port B Your computer will look for drivers for the camera Cancel this process You will load the drivers on your own C Insert the CD from The Imaging Source D The first screen that comes up with the CD inserted will ask you for your language preference Choose your preferred language E The next screen that loads will have 2 choices How to use this CD Software installation F Click on Software installation Three new options will appear Driver Software for end users Software for programmers G Click on Driver Select USB Cameras H The next screen will welcome you to the Driver Installation Wizard Click next after selecting USB Camera if it hasn t already been selected l You may get a screen that indicates that the software you are installing for this hardware has not passed Windows Logo testing Click on Continue Anyway CHAPTER 2 13 J The next screen will indicate the installation is completed Click on Finish Click on Back 2 Installing IC Capture A B IC Capture is installed from Software for end users Click on Software for end users Click on IC Capture This is the first option to select Click on the first option IC Capture This
12. aching and research applications It is not intended for clini cal or critical life care use and should never be used for these purposes nor for the prevention diagnosis curing treatment or alleviation of disease injury or handicap e Do not open the unit the internal electronics pose a risk of electric shock Do not use this apparatus near water e reduce the risk of fire or electric shock do not expose this apparatus to rain or moisture Objects filled with liquids should not be placed on the apparatus Do not block any ventilation openings Install in accordance with the manufacturer s instructions e Do not install near any heat sources such as radiators heat registers stoves or other apparatus that produce heat e Only use attachments and accessories specified by the manufacturer e Unplug this apparatus during lightning storms or when unused for long periods of time This apparatus must be earthed e Use three wire grounding type cord similar to the one supplied with the product Do not defeat the safety purpose of the polarized or grounding type plug A polarized plug has two flat blades one being wider than the other A grounding type plug has two blades and a third round grounding pin The wide blade or the third prong is provided for your safety If the provided plug does not fit into your outlet consult an electrician for replacement of the obsolete outlet e advised that different operatin
13. ers Superfusion Oxygen Buffer Supply Culture Myograph Chamber Waste Bottle Figure 2 4 Illustration of perfusion flow control The superfusion circuit consists of an inflow and an outflow from the culture myograph chamber Both flows are driven by the same peristaltic pump which leads the superfusion buffer from the reservoir to the culture myograph chamber and finally to a waste bottle The small steel pipe on the culture myograph chamber cover is connected to a sterile micro filter 0 20 The small pipe works as a breathing valve to prevent overpressure in the culture myograph chamber The superfusion buffer reservoir is continuously aerated with a mixture of 9576 O and 576 The gas passes through a sterile micro filter 20 to prevent contamination of the sterile superfusion buffer CHAPTER2 17 CHAPTER DMT 202 CONTROL PROGRAM 3 1 Control of Temperature and Light Intensity In addition to the DMT VAS a program is installed on the computer allowing temperature and light intensity control of the Culture Myograph 202 system To run the DMT 202CM control program please select the 202CM controller which was copied to your desktop during installa tion 202CM controller can also be found in START gt All Programs gt DMT gt 202CM NOTE MAKE SURE THAT THE HEAT CONTROLLER IS TURNED ON AND THE MICROSCOPE IS CONNECTED TO THE FRAME GRABBER BEFORE RUNNING THE 202 CONTROLLER 3 1 1 Connecting
14. f the com puter Please see installation instructions in 2 3 Installation of DMT VAS below 1 Ensure that the heat controller is switched off on the rear panel before proceeding with the connection procedure 2 Connect the loose cables from the four culture myograph units to the front panel of the heat controller 3 Connect the power cord to the power inlet on the back panel of the heat controller 4 Connect the Heat Controller to the PC with the loose USB cable using the USB port on the Heat Controller and a free USB port on the PC 5 Connect the DMT Microscope to a USB port on the computer using the fixed USB cord 6 Turn on the power switch of the heat controller f The Power ON LED on the front panel of the heat controller should be lit indicating that the heat controller is on 8 Connect the DSUB 9 pol cable from the DMT microscope to the 9 pol port on the frame grabber card installed in the com puter 9 Turn on the computer 2 3 Installation of DMT VAS This section describes how to install the DMT VAS on your computer along with drivers for the digital USB camera NOTE IF YOU HAVE PURCHASED A COMPUTER FROM DMT ALONG WITH WITH YOUR CULTURE MYOGRAPH 202CM SYSTEM THE DRIVERS AND DMT VAS HAVE ALREADY BEEN INSTALLED FOR YOU 1 Installing the USB camera A Ifa microscope was part of the system 111P 202CM or 204CM then the USB camera is inside the microscope Simply connect the USB connector labeled CAM into
15. g voltages require the use of different types of line cord and attachment plugs Check the voltage in your area and use the correct type See the table below Voltage Line plug according to standard 110 125 V UL817 and CSA C22 2 No 42 220 230 V CEE 7 page VII SR section 107 2 D1 IEC 83 page C4 240 V BS 1363 of 1984 Specification for 13A fused plugs and switched and unswitched socket outlets Protect the power cord from being walked on or pinched particularly at power plugs and the point where they connect to the apparatus Refer all servicing to qualified service personnel Servicing is required when the apparatus has been damaged in any way such as the power supply cord or plug is damaged liquid has been spilled onto or objects have fallen into the apparatus the appara tus has been exposed to rain or moisture does not operate normally or has been dropped SAFETY 5 This equipment has been tested and found to comply with the limits for a Class B Digital device pursuant to part 15 of the FCC rules These limits are designed to provide reasonable protection against harmful interference in residential installations This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instruc tions may cause harmful interference to radio communications However there is no guarantee that interference will not occur in a particular installation If this equipme
16. he DMT VAS application file will open the program If you wish you may create a short cut to this application file and place this short cut on your desktop or in your Start menu 14 CULTURE MYOGRAPH SYSTEM MODEL 202CM USER MANUAL 2 4 Experiment Setup 2 4 1 Connecting the Flow Reservoirs To connect the flow reservoirs with the glass cannulas and the Pressure Regulator DMT recommends PharMed tubes Product No 100127 NOTE A STERILE MICRO FILTER 0 20MM IS CONNECTED BETWEEN THE PRESSURE MANOMETER RESERVOIR AND THE TWO PERFUSION RESERVOIRS TO PREVENT CONTAMINATION OF THE PERFUSION BUFFER Filter Figure 2 2 Illustration of how to connect flow reservoirs CHAPTER2 15 2 4 2 Perfusion Flow Control Regulating the difference in height between the two flow reservoirs allows control of the perfusion flow velocity The principle is illustrated in figure 2 3 below In Figure 2 3 A below the two reservoirs are equal in height and no flow will occur In figure 2 3 B below the difference in height reveals a flow from the right reservoir to the left reservoir NOTE TO ENABLE THE PERFUSION FLOW IT IS IMPORTANT THAT THE RIGHT GLASS CANNULA IS CONNECTED TO THE LONG STEEL PIPE IN THE RIGHT RESERVOIR MARKED BY THE ARROW IN FIGUE 2 3 A BELOW Figure 2 3 Illustration of perfusion flow control 16 CULTURE MYOGRAPH SYSTEM MODEL 202CM USER MANUAL 2 4 3 Setup and Control of Superfusion Flow Peristaltic Pump Micro Filt
17. nt does cause harmful interference to radio or television reception which can be deter mined by monitoring the interference while turning the equipment off and on the user is encouraged to correct the interference by one or more of the following measures e Reorient or relocate the receiving antenna e Increase the separation between the equipment and receiver e Connect the equipment into an outlet on a circuit different to that to which the receiver is connected to e Consult the dealer or an experienced radio TV technician for help APPROVALS Complies with the EMC standards EMC 89 336 EEC EN 50 081 1 and EN 50 082 1 FCC part 15 Class B CISPR 22 Class B Certified with the safety standards EN 60 065 IEC 60065 Complies with the safety standards UL6500 CSA E65 6 CULTURE MYOGRAPH SYSTEM MODEL 202CM USER MANUAL CERTIFICATE OF CONFORMITY DMT A S Skejbyparken 152 8200 Aarhus N Denmark hereby declares its responsibility that the following product Culture Myograph System Model 202CM is covered by this certificate and marked with CE label and conforms with the following standards EN 60 065 IEC 65 Safety requirements for mains operated Electronic and related apparatus for household and similar general use EN 50 081 1 Electromagnetic compatibility Generic emission standard Part 1 Residential commercial and light industry EN 50 082 1 Electromagnetic compatibility Generic immunity standard Part
18. ra Objective Adjustment Light source Voltage Pressure Regulator Pressure range Pressure source Heat controller Temperature range Pump Peristaltic pump gt 60 um One Max 2 ml O D 1 2 mm Built in 10 mm diameter type coverslip Acid resistant stainless steel Removable with gasket and access port Two cannula holders X Y amp Z 25 ml bottles for flow adjustment Ambient temp 50 C Via PC software Included USB 4 B W CCD 10x X Y amp Z directions Infrared LED 100 to 240 VAC auto 50 60 Hz O 300 mmHg Atmospheric pressure 50 2 5 50 rpm for superfusion of the chamber 4 8 channel versions available Optional accessories FlowMeter range 15 ul min to 1500 ul min APPENDIX 2 27 VAS Skejby Science Center Skejbyparken 152 DK 8200 Aarhus N Tel 45 87 41 11 OO aL LIL OL www dmt dk salesedmt dk support dmt dk DMT Asia Ltd Rm 2402B Great Eagle Centre 23 Harbour Road Wanchai Hong Kong S A R P R China Tel 852 6621 8337 Fax 852 3020 7554 www dmt asia com sales dmt asia com support dmt asia com DMT Asia China office Rm 28C No 8 Dong Fang Road Lu Jia Zui Financial District Shanghai 200120 P R China Tel TES O 21 A25 1330 Fax 86 0 21 5877 0063 www dmt asia com sales dmt asia com Support dmt asia com DMT USA Inc 201 East Liberty Street Suite 6 Ann Arbor MI 48104 US
19. raph chamber and glass cannulas 1 Fill up the myograph chamber to the edge with an 8 acetic acid solution and allow it to stand for a few minutes to dissolve calcium deposits and other salt build up Use a swab stick to mechanically clean all chamber surfaces 2 Remove the acetic acid and wash the myograph chamber and glass cannulas several times with double distilled water 3 is hard to remove any kind of hydrophobic reagent used by using step 1 and 2 try incubating the chamber and glass cannulas with 9690 ethanol or a weak detergent solution e g Treepol 4 remove more resistant or toxic chemicals incubate the myograph chamber and glass cannulas with 1M for up to 1 hour In exceptional cases incubate the chamber and supports with a up to 3M HNO solution for about 15 minutes 5 Wash the myograph chamber and glass cannulas several times with double distilled water IMPORTANT NOTES TO STERILIZE THE CULTURE MYOGRAPH CHAMBER AND GLASS CANNULAS USE A STANDARD AUTOCLAVE PROCE DURE BE VERY CAREFUL USING STEP 3 AND 4 REPEATEDLY AS STRONG REAGENTS CAN CAUSE EXTREME DAMAGE TO THE MYOGRAPH UNIT BE VERY CAREFUL NOT TO DAMAGE THE GLASS CANNULAS DURING THE CLEANING PROCEDURE In cases of red or brown discolorations appearing on the chamber sides the following cleaning procedure will work in most cases d Incubate the myograph chamber for 30 minutes with 20 of a 2mM T 1210 Tetrakis 2 pyridylmethyl ethylenediamine sol
20. re the packing material for any possible future transport of the Culture Myograph System In case of transport and the original packing material is unavailable please contact DMT Sales Department for advice and packing instruc tions After unpacking your new Culture Myograph System please use the following list to check that the system is complete 1 Culture Myograph Unit e Culture Myograph Chamber with Chamber Cover e 2 Glass Cannulas Tip outer diameter 125 e 2 Schott Duran Bottles 25ml 2 DMT Microscope e Temperature Probe e Olympus Objective Micrometer including Microscope Objective Holder 3 Culture Myograph Heat Controller Unit USB cable for connection to PC e Power cord The shape of the AC plug varies by country be sure that the plug has the right shape for your location 4 Pressure Regulator 5 Accessories e Small screwdriver 3m nylon thread 2 blind plugs including six O rings 1 07x1 2 mm for the Culture Myograph Chamber e 10 O rings 5 0x1 0mm for fixation of left glass cannula e 10 O rings 18 0x1 2 mm for cover 6 Software amp Manuals e 1 CD with user manual for Culture Myograph System Model 202CM e 1 CD with DMT Vessel Acquisition Suite DMT VAS 7 Peristaltic Pump Optional e Alita Watson Marlow 400 VS2 10R Midi 2 5 50rpm 8 Computer Optional UNPACKING THE MYOGRAPH SYSTEM 9 CHAPTER 1 SYSTEM OVERVIEW 1 1 Culture Myograph Unit Cable to myo Interface e
21. sethe mouse to drag the slider up and down 2 Use Arrow UP and Arrow DOWN to change the IR light intensity with one step 3 Use Page UP and Page DOWN to change the IR light intensity with ten steps 4 Use Home and End to force the IR light fully off or on 5 To manually adjust the IR light intensity adjust the knob on the back of the microscope 204CM Controller E 5 Lonneck Disconnect Exit Chamber 24 1 IH light 100 19 CHAPTER 4 CULTURE MYOGRAPH MANUAL 4 1 The Culture Myograph Unit The culture myograph unit is placed on the DMT microscope as illustrated in figure 4 1 below Press the black lever to lock the culture myograph unit onto the DMT microscope 4 1 1 Glass Cannula Adjustment The glass cannulas are adjustable in all X Y Z directions The adjustments are illustrated in figure 4 1 below Horizontal adjustment gt please see fig 4 2 1 Vertical adjustment 4 gt nz E Longitudinal adjustment lt e e Myograph unit lock Figure 4 1 Illustration of glass cannula adjustment 20 CULTURE MYOGRAPH SYSTEM MODEL 202CM USER MANUAL Horizontal Adjustment Horizontal adjustment is performed as illustrated in figure 4 1 on previous page and figure 4 2 below Gently loosen the screw fixing the chamber and carefully move the myograph chamber in
22. the Heat Controller and the Microscope When the 202CM Controller is started it will automatically connect to the Micro scope and Heat Controller In the About Tab the status of the COM connections is shown If the connection fails please check that the Microscope and Heat Controller is turned on and that the USB cables is connected Set temperature The temperature controller tab is used to adjust the set temperature for each chamber range 10 45 C with O 1 C accuracy If a temperature is changed click the button Change and the change is up loaded to the heat controller The setting is permanently stored in the heat controller Status Status indicates status for each chamber Myograph is not connected to the heat controller d The Myograph is connected The heat has not reached set temperature Ej The Myograph is connected The heat has reached set temperature 18 CULTURE MYOGRAPH SYSTEM MODEL 202CM USER MANUAL Hi 204CM Controller E J X Connect Disconneck Exit Controller Version 1 3 Microscope ls COMS Heat controller Is on COM Connect Discanneck Exit SET STATUS Chamber 1 Chamber 2 a0 Chamber 3 0 Chamber 4 0 Change Actual Temperature The microscope controller tab shows the actual temperature measured in the chamber placed on the microscope Light Intensity To change the light intensity use the track bar The options are as follows 1 U
23. u tion dissolved in double distilled water Use a swab stick to mechanically clean all the affected surfaces during the last 15 minutes of the incubation period Wash the myograph chamber several times with double distilled water Incubate the myograph chamber with 96 ethanol for 10 minutes while continuing the mechanical cleaning with a swab stick Remove the ethanol solution and wash a few times with double distilled water Incubate the myograph chamber with an 896 acetic acid solution for 10 minutes and continue the mechanical cleaning with a swab stick Wash the myograph chamber several times with double distilled water CHAPTER 4 25 APPENDIX 1 FUSE REPLACEMENT The main fuse of the myograph system is placed inside the power inlet on the Heat controller If the fuse blows it is easily changed using the following procedure IMPORTANT IF THE FUSE NEEDS TO BE CHANGED MAKE SURE THAT THE REPLACEMENT FUSE IS EQUAL TO THE ONE BLOWN Specifications T2A 250 6 3 x 32mm Use a small screwdriver to open the voltage selector block Red Fuse Block 26 CULTURE MYOGRAPH SYSTEM MODEL 202CM USER MANUAL APPENDIX 2 SYSTEM SPECIFICATIONS Technical specifications Chamber Unit Vessel size Chambers Chamber volume Cannula ports Superfusion ports Base window Chamber material Chamber cover Chamber Stage Alignment Adjustment Flow Temp range Temp control Temp probe Microscope Stand Came
24. ula from the culture myograph unit Then carefully unscrew the locknut and remove both locknut and glass cannula Removal of Culture Myograph Chamber After removal of both glass cannulas loosen the horizontal adjustment screw to free and remove the chamber from the culture myograph chamber Mounting of all parts is performed the same way but in opposite direction 22 CULTURE MYOGRAPH SYSTEM MODEL 202CM USER MANUAL 4 1 3 Changing O rings Each locknut on the culture myograph chamber is fitted with rubber O rings 61 07 x 1 2 7 mm to keep the chamber tight These O rings will from time to time need to be replaced The procedure is illustrated in figure 4 4 below Figure 4 4 Changing O rings e Unscrew the locknut to reveal the O ring and remove it carefully using small forceps or a similar tool e Insert the new O ring into the chamber hole and push it back until it makes contact with the bottom of the hole e Carefully insert the glass cannula through the locknut and place it in the chamber hole Gently tighten the locknut NOTE BE CAREFUL NOT TO DAMAGE THE GLASS CANNULA TIPS WHEN DISASSEMBLING THE CULTURE MYOGRAPH CHAMBER CHAPTER 4 23 4 2 The DMT Microscope The DMT microscope is an invert microscope equipped with a Zeiss Achromat 10X 0 25 objective and build in digital USB CCD camera The objective is adjustable in all X Y Z directions using the three micro positioners on the front as illustrated in figure 4 5 below
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