Operator`s Manual
Contents
1. is in the locked position The plug of the power supply cord might not be in a live wall outlet or the power supply connector might not be fully inserted in the AC Input Receptacle of the Bullet Blender If the unit stops working turn the system off for 15 minutes to allow the NEXT gt gt electronics to reset If the Bullet Blender does not turn on after this period contact customer service If the caps on the microcentrifuge tubes pop open make sure that the interface regions between the lids and the caps is dry when you close the caps or screw them on so that there is enough friction for the caps to remain tight Using recommended types of tubes will minimize tube opening If the 4 C light is not coming on frost from condensation may be blocking the air passage Remove the inner dry ice compartment and gently agitate it or replace the ice If mist coming out of the dry ice cooling container seems excessive make sure that the lid of the dry ice container is closed securely SUPPORT FAQs protocols and other helpful information are available on our website http www nextadvance com Click on the Bullet Blender then on the appropriate link If you cannot find an answer there please contact customer service by email at techsupport nextadvance com or by telephone at 518 674 3510 or 800 738 1681 un ADVANCE Operator s Manual for the Bullet Blender Gold SPECIFICATIONS Size 35 cm 14 in de2. parts Evidence of purchase by the original purchaser is required Next Advance may also request documentation of proper maintenance if applicable Next Advance makes no other warranty express or implied with respect to its Products NEXT ADVANCE MAKES NO WARRANTY RESPECTING THE MERCHANTABILITY OF THE PRODUCTS OR THEIR SUITABILITY OR FITNESS FOR ANY PARTICULAR PURPOSE OR USE Next Advance shall not be liable for indirect special incidental or consequential damages of any nature Any recovery for any claim shall be limited to the original purchase price for the product Operator s Responsibility Provide proof of purchase and provide normal care and maintenance WARNINGS AND CAUTIONS Read the users manual before operating 6 ADVANCE Operator s Manual for the Bullet Blender Gold Do not carry the instrument by the dry ice compartment Instead lift from the bottom sides of the instrument Do not operate Bullet Blendere units with empty tubes Do not open lid or cover when the Bullet Blender is in use Wear cryogenic gloves when handling dry ice Do not use the inner container of the dry ice cooling compartment to transport ice Do not use regular water ice in the dry ice cooling compartment Do not insert fingers or objects other than recommended tubes into sample tube holes Use caution when closing Bullet Blender lid do not close on fingers Use recommended tubes only No user service
3. Operator s Manual The Bullet Blender Gold BB24 AU Congratulations Congratulations on your purchase of a Bullet Blender Gold by Next Advance Inc for lysing disrupting and homogenizing your samples Please read this operator s manual which explains proper operation of the instrument This manual is posted on our website www nextadvance com Click the SUPPORT button on the left hand menu bar and then on the appropriate link to the manual We re confident that your Bullet Blender will become an essential tool in your laboratory and we wish you success with your work Contents Parts of the Bullet Blender 2 SI Dio RON CODEE IDOL icecaosseoE 1 ot229250672923666010 00590954 Protocols and Sample Settings 4 ABI STO ILIO RON 5 Troubleshooting sssse 5 DU OI AA 5 SPeCCIfICATIONS eeeeceeeeeeeeeeeceeeeeeeeeeeees 6 W ruris M Warnings and Cautions 6 BYTE SRI DO 7 Contact Info NEXT gt gt ADVANCE Operator s Manual for the Bullet Blender Gold Parts of the Bullet Blender Gold Instrument Cover sample Plate Cover Sample Plate Dry Ice Cooling Compartment Lid Gasket Sample Tubes Dry Ice Cooling Compartment SYMBOLS USED ON THE BULLET BLENDER GOLD Start Operation This product complies with European Low Voltage and EMC Directives A Caution Follow the Instructi
4. able parts are inside For indoor use only Pollution Degree 2 per EN 61010 1 Overvoltage Category II per EN 61010 l Enclosure Protection Not Protected Against the ingress of Moisture Sound Pressure Level Up to 90dBA Use hearing protective devices that reduce exposure to below 85 dBA during prolonged exposure Do not immerse in liquid NEXT gt gt Before touching the Bullet Blender touch a bare metal surface to discharge static electricity DISCLAIMER Next Advance Inc representations or warranties expressed statutory or implied regarding the fitness or merchantability of this product for any particular purpose In no event shall Next Advance be liable for incidental or consequential damages Next Advance Inc is not liable for any damages including but not limited to lost profits lost savings or other incidental or consequential damages arising from ownership or use of this product or for any delay in the performance of its obligations under the warranty due to causes beyond its control Next Advance Inc also reserves the right to make any improvements or modifications to the product described in this manual at any time without notice of these changes Next Advance Inc products are not designed intended or authorized for use in applications or as system components intended to support or sustain human life as a clinical medical device for humans or for any application in which the failu
5. ep x 46 cm 18 in wide x 38 cm 15 in high Weight 30 lbs Power Requirement 100 240 V 50 60 Hz 2 5 4 5 A Capacity 24 of 1 5 mL RINO tubes or Eppendorf Safe lock tubes Relative Humidity 5 90 condensing non Operating Temperature 4 40 C Altitude lt 2000m Storage Temperature 40 to 50 C Meets C requirements CE models only WARRANTY Next Advance warrants its Products against defects in materials and workmanship for time periods which vary according to the Product Within these time periods Next Advance will replace or repair without charge to the original purchaser any part which is defective Bullet Blender Two years The warranty is void if the Product is defective due to product accident product modification exposure to radiation other than for sterilization connection to an improper electrical supply lack of proper maintenance contamination improper installation or NEXT 22 misuse If the product is used in a manner not specified by the manufacturer the protection provided by the equipment may be impaired The warranty shall also not apply to defects arising from fire flood lightning or other conditions unrelated to correct operation of the Product Next Advance s liability is limited at the company s election to 1 refund of the original purchaser s purchase price for the Product 2 repair of the Product or 3 replacement of the Product or defective
6. handling dry ice Do not use the inner portion of the dry ice cooling compartment to transport the ice Do not fill the dry ice cooling compartment with water ice Make sure that the dry ice cooling compartment lid is properly sealed To pre cool the instrument after filing the compartment with dry ice run the instrument for 5 minutes or until the 4 C light comes on with no tubes inside To use Air cooling M where ambient air is drawn into the instrument operate the Bullet Blender Gold with the dry ice compartment NEXT gt gt open Air Cooling is most effective when the instrument is operated in a 4 C environment PROTOCOLS AND SAMPLE SETTINGS The following ratio should be used as a guideline for determining the amount of beads and buffer to use given a certain sample size 1 volume mass of tissue 1 volume of beads 2 volumes of buffer For more specific information regarding the use of various beads as well as specific protocol information please refer to our website www nextadvance com As the tissue amount becomes smaller the above recommended ratio may differ due to the limitations of handling of the small volumes We recommend using a minimum of 25 uL of buffer regardless of your sample size The maximum sample mass is 300 uL per microcentrifuge tube in the Bullet Blender Gold and the tube should not be filled more than two thirds of the way this includes sample beads and buffer The mechanics
7. of homogenization require empty space in the tube Cutting the tissue into smaller pieces will generally yield better results Tissue with a high aspect ratio long thin strips will homogenize better than tissue that is round or cubic Do not operate the Bullet Blendere Gold using the same tubes for longer than 15 minutes Protocols for many types of samples are posted on our website at 4 ADVANCE Operator s Manual for the Bullet Blender Gold www nextadvance com FAQs protocols h tm Notes At high speed settings there may be some flaking of the tubes This is a normal side effect of homogenization The higher speed enables homogenization of tougher tissue CLEANING If you wish to clean your Bullet Blender clean the outside of the unit only with mild soap water and a soft cloth Under normal conditions the Bullet Blender Gold should never need to be disassembled for cleaning In the case of a large spill unplug the instrument remove the sample tube plate with an 1 8 hex wrench wipe out the spill using standard laboratory safety precautions and replace the sample tube plate Do not touch or tamper with the electronics TROUBLESHOOTING In addition to the tips given below a thorough list of troubleshooting tips is at http www nextadvance com FAQs FA Q s Bullet Blender htm If the Bullet Blender doesn t start make sure the Power Switch is in the ON I position Make sure that the sample plate lid
8. ons in the Operator s Manual Please dispose the test tubes and the BBX or BBY Bullet Blender in accordance with local regulation 1 E H F u NO NEAT 22 ADVANCE SETUP Place the Bullet Blender Gold on a stable level lab bench Carry it by grasping the bottom sides Plug the AC power supply cord connector into the AC Input Receptacle on the right side of the Bullet Blender and then insert the plug into a wall outlet You should see lights on the time setting flash for several seconds indicating that the unit is receiving power It is now set up OPERATION To use your Bullet Blender Gold lift open the instrument cover twist the handle of the sample plate cover counter clock wise until the white arrow is in the unlocked position see figure below and lift the sample plate cover out of the instrument Insert the sample tubes evenly spaced re place the sample plate cover twist the handle clock wise until it is in the locked position and close the instrument cover The figure above shows labels that indicate if the sample plate cover is in NEAT 22 the locked or unlocked position Here it is shown in the locked position Only 1 5 mL RINO Screw cap tubes or Eppendorf Safe Lock snap cap tubes should be used in this instrument In order to use these tubes the Bullet Blender must be fitted with the appropriate gasket corresponding to the tube type Each gasket is clearly labeled RINO or Ep
9. pendorf The gasket is removed by unscrewing the three screws with a Phillips head screw driver and lifting the gasket off of the lid Attach the proper gasket by aligning the holes in the gasket with the holes in the lid and inserting the screws Make sure that the gasket is screwed on securely or else homogenization efficiency may be affected Only RINO screw cap tubes are recommended for use in the Bullet Blender outfitted with the RINO gasket other screw cap tubes may break or result in sub optimal homogenization For best results the tubes should be evenly spaced Set the duration in minutes and the speed to the desired value Push the start button As each minute passes another LED will light up The Bullet Blender Gold will not start until the sample plate cover is properly locked If you turn the Time knob to 0 the instrument will stop ADVANCE Operator s Manual for the Bullet Blender Gold The figure above shows the Bullet Blender Gold set to run for 4 minutes at a speed of 8 after running for 2 minutes To operate press the START button After 15 seconds the LED light by the number 15 will light up After 30 seconds the second LED light by the number 30 will light up And so on At the settings shown above after 4 minutes the Bullet Blender Gold will stop To use 4 C cooling fill the dry ice cooling compartment with at least 2 Ibs of dry ice Use cryogenic gloves when
10. re of the product could create a situation where personal injury or death may occur All brand and product names used in this manual are the trademarks of their respective owners NEXT ADVANCE INC DOES NOT makes no ADVANCE Operator s Manual for the Bullet Blender Gold GUARANTEE THE INTEGRITY OF THE TUBES USED IN THE BULLET BLENDER TUBES THAT ARE NOT RECOMMENDED BY THIS MANUAL MAY CRACK OR OPEN IF USED IN THE BULLET BLENDER NEXT ADVANCE INC OPTIMIZES THE BULLET BLENDER TO SPECIFIC TUBE TYPES AND BRANDS AND CANNOT GUARANTEE THE SAFE USE OF ALL TUBES BEING SOLD ON THE MARKET CONTACT INFO Next Advance Inc Averill Park NY USA Telephone 518 674 3510 www nextadvance com support nextadvance com NEXT 22 ADVANCE OO
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