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X-Cite® XLED1 User Guide

1. lt lt lt lt Figure 13 LED Trigger Screen a sisK cua tku ER a uU Figure 14 Trigger Graphs Screen GUI Figure 15 Maintenance Screen GUI 121 111 Figure 18 Dichroic Database GUI isee cn ensn eu neu n uu in ux cx eu eR ua cR uu ER ug Cx Cx ER E CREER ER EE CREE EREE CERE ERE Figure 19 Alarm Code Screen e LUMEN DYNAMICS lll 035 00465R rev 5 X LCire Fluorescence Illumination In Control Figure 20 System Information nennen nennen 36 Figure 21 Log In Screen Touch Screen Controller essere 38 Figure 22 LED Screen Touch Screen Controller eeeeeeee 39 Figure 23 LED Name Edit Screen Touch Screen Controller 41 Figure 24 LED Trigger Screen Touch Screen Controller 42 Figure 25 Trigger Graphs Screen Touch Screen Controller 45 Figure 26 Maintenance Screen Touch Screen Controller
2. 46 Figure 28 Alarm Code Screen Touch Screen Controller 48 Figure 29 System Information Touch Screen 51 Figure 30 Terminal Block Connectors nennen nenne nennt nnns 55 Figure 31 Locking UD 59 Figure 32 LED Module and Driver Replacement 61 Figure 33 Dichroic 63 Tables Table 1 Front Panel LED Indicators 2 22 2 8 Table 2 Alarm Codes GUN 34 Table 3 Error Pop up Screen Descriptions 0 35 Table 4 Error Window Descriptions GUI 2 2 2 9 37 Table 5 Alarm Codes Touch Screen Controller 4 10 48 Table 6 Error Pop up Screen Descriptions Touch Screen Controller 49 Table 7 Error Window Descriptions Touch Screen Controller 52 Table 8 External Trigger Pin 56 Table 9 External Sync Out Pin As
3. Fluorescence Illumination In Control X Cite XLED1 User Guide Lumen Dynamics Group Inc 2260 Argentia Road Mississauga ON L5N 6H7 Canada 1 905 821 2600 www LDGI com 035 00465R Rev 5 LUMEN DYNAMICS Fluorescence Illumination In Control User Guide 035 00465R Lumen Dynamics Group Inc 2011 2012 All rights reserved No part of this publication may be reproduced transmitted transcribed stored in a retrieval system or translated into any language in any form by any means without the prior written consent of Lumen Dynamics Group Inc Every effort has been made to ensure information in this manual is accurate however information in this manual is subject to change without notice and does not represent a commitment on the part of the authors Trademarks X Cite is a registered trademarks of Lumen Dynamics Group Inc All rights reserved All other product names are trademarks of their respective owners Any product or software photos shown are for reference only and are subject to change without notice Printed in Canada Doc No 035 00465R No part of this publication may be reproduced transmitted transcribed stored in a retrieval system or translated into any language in any form by any means without the prior written consent from Lumen Dynamics Group Inc Every effort has been made to ensure information in this manual is accurate however information in this manual
4. e Zero Potential Most importantly make sure you and the system are at Zero Potential by continuously touching an un painted metal surface of the chassis or the grounded table e Standing It is also very important that you are standing at all times when working on the system Sitting on a chair can generate more electrostatic energy e Cords Make sure everything is removed from the back of the system power cord touch controller interface cables etc e Clothes Make sure not to wear any clothing that conducts Electrical Charge such as a wool material e Weather Electrical storms can increase the ESD risk unless absolutely necessary try not to work on the system during an electrical storm e Accessories To help reduce ESD and help prevent other problems it is also a good idea to remove all jewellery With this basic understanding of ESD and its impact on your environment you can then begin to develop an effective ESD control program by reducing as many static generating processes or materials as possible such as the contact and separation of dissimilar materials and common plastics from the work environment Keep other processes and materials at the same electrostatic potential Electrostatic discharge does not occur between materials kept at the same potential or at zero potential Therefore provide ground paths such as a wrist strap or a conductive surface connected to ground to allow static accumulating in yourself and o
5. Fluorescence Illumination In Control 3 2 XLED1 Back Panel LOCKING HUB KEY HANGER RECOMMENDED LOCATION eel AC CONNECTION FUSE 2 nian PORT ToUCH TRAY CREEN CONTROLLER LOCKING HUB KEY HOUSING OPTIONAL LOCATION Figure 2 XLED1 Back Panel Image LUMEN DYNAMICS O 035 00465R rev 5 x lt Cite Fluorescence Illumination In Control 3 3 Optional Touch Screen Controller Swivel Stand TOUCH SCREEN SWIVEL LEG Figure 3 Touch Screen Controller 3 3 1 optional Touch Screen Controller has a stand made up of two separate extendable legs that swivel out from the back of the Touch Screen Controller Refer to Figure 3 3 3 2 swivel the legs out push in the green X Cite button and swivel the leg out to one of the pre set stops to achieve the optimal viewing angle Repeat for the other leg 3 3 3 Toretract the legs push in the green X Cite button and swivel the legs back into the body of the controller LUMEN DYNAMICS 1 035 00465R rev 5 Fluorescence Illumination In Control 4 Installation Procedures 4 1 General Installation oN Note Before any installation occurs ensure there is a minimum space allowance of 50mm surrounding XLED1 to maintain acceptable airflow performance Airflow is exhausted from the rear of XLED1 4 1 1 Lumen Dynamics recommends installing the locking hub key hanger Bend the flexible tang into a hook shape peel off the protecti
6. Fluorescence Illumination In Control Error Pop up Screens Descriptions Error Window Error Description Screen Location Light Guide not inserted Request to turn on LED could not be completed due to Light Guide not fully inserted All screens Enter Delay or Off Time Request to start Pulse Generator cycle could not be completed due to missing delay or off time entry Trigger screen To change units or trigger LED must be off Requested settings change was not completed as the LED was on Trigger screen User ID is incorrect Entered incorrect User ID Login screen Retry or enter as Guest Entered incorrect User ID Login screen Number is out of range The number value entered is out of range Number input pad Alarm Active on LED Proceed to maintenance screen Request to turn on an LED could not be completed due to an active alarm Main screen Dichroic not found Wrong Dichroic number entered Maintenance screen PIN already in use If PIN is the same as another user s During PIN update wizard Please choose another If PIN is the same as another user s During PIN update wizard PINs do not match If the second PIN does not match the first During PIN update wizard Guest cannot change PIN If change PIN button is pressed and the current user is Guest Maintenance screen
7. See section 18 for additional warranty coverage Lumen Dynamics warrants the original purchaser for a period of one 1 full year calculated from the date of purchase that the equipment sold is free from defects in material and workmanship repairs are warranted for 90 days In the event of a claim under this warranty the equipment is to be sent postage and carriage paid to the Lumen Dynamics Service Center Returned equipment will not be received without a Return Authorization RA Number issued by the appropriate Service Center In order for us to serve you better include a written description of the fault and the name and telephone number of a contact person who may be contacted for additional service related questions Any claims for units received with defects in material or workmanship must be reported to an authorized Lumen Dynamics Service Center within 30 days from the original date of receipt and returned within 30 days of reporting to a an authorized Lumen Dynamics Service Center Lumen Dynamics will repair or replace these reported defects free of charge The equipment must be sent postage and carriage paid Package the equipment in its original shipping case or as appropriate to prevent damage during transport In the case of damage caused by wear and tear careless handling neglect by the use of force or in the case of interventions and repairs not carried out by a Lumen Dynamics Authorized Service Center the warranty
8. 3 Each LED module section has a coarse intensity adjustment slider and fine intensity adjustment UP A and DOWN pads The fine intensity adjustment arrows allow intensity adjustments from 5 to 100 in 0 196 increments A numerical value can be entered by clicking on the 96 value to access the popup Edit Entry window Enter a number and press OK The intensity will now be changed e LUMEN DYNAMICS 2 6 035 00465 5 X LCire Fluorescence Illumination In Control 6 1 4 main screen has a global LOCK ALL LEDS this button sets all the individual lock buttons to locked 6 1 5 main screen has a global TURN OFF ON ALL LEDS pad which will disable and enable all LEDs at once 6 1 6 main screen displays Icons for Light Guide Inserted Alarms and LED 6 1 7 Click on the LOGOUT button to close the GUI Control Panel All previous data entered will be saved upon next start up 6 2 Set LED Triggers Screen es Touchscreen Locked Light Guide Inserted Alarm LED on VIEW TRIGGER GRAPH Repeat Loop ON F TURN ALL 5 RETURN LED SCREEN MAINTENANCE Figure 13 LED Trigger Screen GUI 6 2 1 screen displays individual Trigger window sections for each LED module e g UVX BDX BGX and RLX Click the top of a wavelength section to open that selected window 6 2 2 n each window section the user can change th
9. 3 audible beeps are only generated when the Touch Screen Controller is connected Table 6 Error Pop up Screen Descriptions Touch Screen Controller LUMEN DYNAMICS 49 035 00465R rev 5 X Cite Fluorescence Illumination In Control 7 8 1 8 visual alarm indicators on the Alarm Screen refer to Figure 28 are indicators only and do not function as alarm clearing buttons pads 7 8 2 The LED System is designed to detect certain conditions that may affect safe or proper functioning These conditions are categorized as one of two alarm types according to the danger they pose to the operator system or experiment 1 Critical Do not continue process alarm shall not be clearable until Alarm Condition is corrected Contact http www ldgi xcite com asc php 2 Major operation may continue but depending on specific alarm condition functionality of unit may be limited or impossible Contact http www ldgi xcite com asc php 7 8 8 Alarm Information screen displays touch pads for the alarm codes which will light up and generate an audible signal when triggered 7 9 Clearing alarms 7 9 1 8 visual alarm indicators on the Alarm Screen refer to Figure 28 are indicators only and do not functional as alarm clearing buttons pads 7 9 2 system provides an audible alarm to alert the operator of Light Guide not inserted condition only 79 3 The audible alarm cannot be silenced and will generate
10. 3 audible beeps only 7 9 4 The following visual alarms will clear automatically when the problem subsides or is corrected Max Temp Exceeded Min Temp Under Limit LED Module Fail Light Guide Not Present e LUMEN DYNAMICS 5 035 00465R rev 5 X Cite Fluorescence Illumination In Control 7 10 System Information os Touchscreen Locked Light Guide Inserted Alarm em LED on Errors Software Versions 1 4 0 1 1 0 1 1 0 ALIGN Serial Number TOUCH SCREEN POWER ON SELF TEST 00129 RETURN TO MAINTENANCE SCREEN Figure 29 System Information Touch Screen Controller 7 10 1 System Information screen Shows an overview of the complete system Touch Screen Controller and XLED1 Errors Window gt Displays error description sent by the XLED1 detected during the power on self test POST and errors detected during operation Refer to Table 7 for error window descriptions Software Versions Window gt Displays the current software versions installed in the XLED1 Pulse Generator Touch Screen Controller Serial Number Window gt Displays the serial number of the XLED1 LCD Settings Window gt This screen allows adjustments to the Volume alarm Touch Screen Controller screen Brightness and the Touch Screen Controller screen saver Timeout Drag the sliders up or down for desired settings gt To disable the Timeout sleep mode set slider to 0 L
11. D XLED1 System Power Consumption 420W max Supply Universal input 100 240Volts AC 50 60Hz 100V 4 2A max 240V 1 8A max Fuse Rating F6 3A 250V X2 Front Panel Indicators Light Guide Indicator Green Light Guide Present Red Light Guide not Present LED ON OFF Button Indicator Green any LED module is ON OFF all LED Modules are OFF Front Panel Switches LED ON OFF green illumination ON LED ON OFF no illumination OFF AC Power Switch Front Panel Ports Light Guide Optical Tube Rear Panel Ports AC inlet 3 terminal ground USB type B female supports 2 1 and below Trigger Input BV 6 5mA max Sync Output 5V 100 max Touch Screen Controller Port e LUMEN DYNAMICS 72 035 00465R rev 5 X LCire Fluorescence Illumination In Control Internal Optical Layout 4 port optical table with capacity for up to 4xLED Modules Dichroic Combiner Modules 4x Driver Modules Triggering Internal External User defined per channel Global trigger input User defined free running or single shot pulse modes Internal triggering generated from Pulse Generator Module min pulse width 10us max 18 hours Sync out with delay options Switching Speed Between wavelengths instantaneous Pulsing single wavelength 100 Overall Dimensions 216mm W x 387mm D x 205mm H Weight 7 5kg 16 5lbs max Maximum Noise Level 49dBA at a distance of 1m Optional Touch Screen Controller Scre
12. LUMEN DYNAMICS 2 8 035 00465R rev 5 X LCire Fluorescence Illumination In Control a In internal triggering mode the LEDs in the same time domain a time domain is either seconds milliseconds or microseconds will be automatically synchronized The LED period determined by adding the Delay Time On Time and Off Time will be adjusted to the longest period selected In the example shown in graph LED A below the total period is 6 seconds Then as shown in graph LED B below the period defined by the user is 4 seconds In this case an additional 2 seconds will be added to adjust the time period to 6 seconds to match that of LED A b In internal triggering mode if the period of one LED is less than 2 times the period of another LED in the same time domain the system will generate additional cycles to compensate for the longest period in the time domain Example In graph LED A below the period is 6 seconds In graph LED C the period specified was 2 seconds The software will automatically create three cycles to fill in the same period as LED A If this is not desired the off time for LED C needs to be extended so that the LED C total period will be equal to that of LED A In this example change the off time from 0 seconds to 4 seconds in order for the total period time to equal to 6 seconds NOTE The additional pulses can only be added up to a maximum which depends on the number of channels being used in the same time domain Typ
13. Operating the GUI Control lt lt lt 2 lt lt lt 64 Main LED Sree oriana aa es bet vase a a D E a aR 5 2 Set LED Triggers SCreen 6 3 Trigger Graphs Screen 6 4 Maintenance inrer ensis snis en nennen 6 5 niece te sss eerte e eta rette taa Pel ter dra 6 7 Glearng alatms uiia Let iar ead dt rtr cod brit reddet b E ru Eck ee de eren des 6 8 System Information iiir tde EE Liter eec erae dee ea hd ei 7 Operating the Optional Touch Screen Controller r EU Kore E oeste 7 3 Main LED SCreen 1 LA Edit LED Name tte irre Eten t Eee 7 5 Set LED Triggers SCEGel Do 7 6 Trigger Graphis SCIeer cue rete Perdre oy ua nun b de aga 7 7 Maintenance SCreehn ees tese Eier addet i ee Ada eR Fase 7 8 Alarm Information icit eerie 7 9 Clearing niei Eo paite ot up 7 10 System Information 8 Live Cell Mode on Live C
14. again and enable Live Cell Mode through the front panel button the last saved settings chosen LEDs intensity and pulse duration will be used for pulsing 8 2 3 4 NOTE If it is desired to save the changes when the XLED1 touch screen controller is not connected login as user number 5 If touch screen controller is plugged in the last change made before logging out settings are only saved on logout will take effect 8 2 4 Using the GUI 8 2 4 4 Changes can be made to duty cycle and intensity as described above 8 2 4 2 When LIVE CELL mode is enabled these changed will apply when the LED s are turned ON 8 2 4 3 When logging out of the GUI make sure to SAVE SETTINGS Note This option only appears if the XLED1 touch screen controller is not connected 8 2 4 4 When the unit is turned on in the future and live cell mode is enabled the last trigger screen settings and LED intensities are applied e LUMEN DYNAMICS 54 035 00465R rev 5 Fluorescence Illumination In Control 9 External Control 9 1 External Trigger Interface SYNC OUT PORT L TRIGGER INPUT PORT ao TRIGGER USB Figure 30 Terminal Block Connectors 9 1 1 rear panel of the XLED1 refer to Figure 30 has two HD15 connectors One 15 pin HD15 connector for input triggers refer to Table 8 and one 15 pin HD15 connector for sync output refer to Table 9 LUMEN D
15. defined by the user is 4 seconds In this case an additional 2 seconds will b added to adjust the time period to 6 seconds to match that of LED A e LUMEN DYNAMICS 43 035 00465R rev 5 X LCire Fluorescence Illumination In Control b In internal triggering mode if the period of one LED is less than 2 times the period of another LED in the same time domain the system will generate additional cycles to compensate for the longest period in the time domain Example In graph LED A below the period is 6 seconds In graph LED C the period specified was 2 seconds The software will automatically create three cycles to fill in the same period as LED A If this is not desired the off time for LED C needs to be extended so that the LED C total period will be equal to that of LED A In this example change the off time from 0 seconds to 4 seconds in order for the total period time to equal to 6 seconds NOTE The additional pulses can only be added up to a maximum which depends on the number of channels being used in the same time domain Typically only 12 sub pulses can be generated if more are needed try using different time domains to achieve the desired results 2 sec 2 sec 2 sec User Specified 4 LED A Period 6 seconds gt System Addition 1 sec 2 sec 1 sec 2 sec LED B Period 4 seconds LED B ext 2 sec gt LED C Period 2 seconds repeated 3 times L
16. empty X Cite XLED1 unit yellow Dichroic location blue LED module and driver location For LED Module Driver and Dichroic Replacement instructions refer to sections 10 and 11 Figure 4 LED Module Driver and Dichroic Locations CAUTION To protect the LED Module Driver and system from electrostatic discharge ESD related damage which can disable the system cause latent defects or permanent LUMEN DYNAMICS 1 2 035 00465R rev 5 X Cite Fluorescence Illumination In Control damage or require repair by service technicians please take the following ESD prevention steps e Ensure the unit is unplugged Ensure you are properly grounded ideally by using an ESD grounding strap attached to an ESD protective surface Refer to section 2 3 for principles of ESD and additional ESD precautions Tools Required Locking Hub Key supplied 2 Philips Head Screwdriver not supplied Torque tool not supplied LED Modules Drivers and Dichroics are shipped in ESD bags LED Modules are shipped with a protective lens cap covering the LED lens 4 2 1 Ensure the system is OFF and the AC power cord is unplugged from the power source 4 2 2 Using the locking hub key supplied rotate the green locking hubs 90 degrees and remove the locking hubs Holding the XLED1 housing at the back slide the XLED1 housing up and back away from the fascia for removal The locking hub key can be stored on the lock
17. frame assembly Improper handling and cleaning practices can damage surfaces or coatings which are utilized in this assembly Note that damage to the surface of the Dichroic coating can degrade the light output performance Proper handling and cleaning technique should be adhered to Below are the recommended procedures for cleaning of the Dichroic LUMEN DYNAMICS 66 035 00465R rev 5 X LCire Fluorescence Illumination In Control Handling e Always handle the Dichroic by the metal frame never touch the Dichroic optical window assembly with your fingertips The moisture or oils on your fingertips can damage the coating of the glass in the optical window Fingerprints left on an optical surface for an extended period of time can become a permanent stain Even if you are wearing gloves avoid touching the optical surface Never handle the Dichroic optical window with metal implements or tweezers e Tostore Dichroics wrap them individually in a clean protective ESD bag and store in a safe dry place e Never store multiple unwrapped Dichroics in the same bag as they may become damaged if they come in contact with each other Cleaning Materials The following materials are recommended for cleaning the Dichroic e Rubber Bulb Dust Blower non pressurized e Lint free lens tissue lint free cotton swabs lint or powder free gloves or finger cots e Anorganic solvent such as reagent grade isopropyl alcohol or lens cleaning so
18. green lit module and compare pop up screen edge value to the actual printed value If the optional Touch Screen Controller display does not illuminate 1 Verify that the Touch Screen Controller is plugged into the XLED1 and the XLED1 turned on 2 Verify the screen brightness is turned up section 7 10 Touch Screen Controller System Information Screen 3 Verify screen Time out mode setting section 7 10 Touch Screen Controller System Information Screen Touch Control Screen Accuracy 1 If the touch control is lagging navigate to the System Information Screen to run screen calibration for screen accuracy follow these steps LOG IN Main LED Screen Press the Maintenance Screen pad Press the System Pad Press the Align Touch Screen Pad refer to section 7 10 e LUMEN DYNAMICS 7 1 035 00465 5 X LCire Fluorescence Illumination In Control 14 Technical Specifications LED Modules Central Wavelength FWHM Refer to http www Idgi xcite com products led Modules php for available wavelengths Dimensions 50mm W x 72mm D x 71mm H Connections HD DB 15 style through connector on module underside Driver Modules Quantity 2 types high power and standard power Max Power Delivery 75W per driver 12 5V max 14A max Dimensions 71mm W x 33mm D x 65mm H Connections DB 25 style through connector on module underside Dichroic Combiner Modules Dimensions 38mm W x 55mm H x 3mm
19. is experiencing problems LED lt wavelength gt ADC error Internal error LED lt wavelength gt Type Mismatch LED Module LED driver types are mismatched Table 7 Error Window Descriptions Touch Screen Controller r Note The alarm will not be triggered if an LED fails when the intensity is set to under 1096 To check if an LED has failed in this case simply move the intensity slider to 100 to check if it is functional WITH CAUTION LUMEN DYNAMICS 5 2 035 00465R rev 5 X LCire Fluorescence Illumination In Control 8 Live Cell Mode on Live Cell Configuration units Live Cell Mode is a feature embedded in XLED1 Live Cell Configuration units includes modules BLX BDX BGX and GYX This feature allows any enabled LEDs together or individually to switch between pulsed and continuous mode of operation through the LED ON OFF button on the front of the unit NOTE It is NOT recommended to have both the touch screen controller and GUI connected to the XLED1 at the same time 8 1 Enabling Live Cell Mode 8 1 11 Hold down the LED ON OFF button for at least 5 seconds until light guide indicator light turns orange 8 1 2 enabled pressing the LED ON OFF button will cause all 4 LEDs to turn on at 10096 power and pulse at the default duty cycle of 5096 with a period of 2ms i e 1ms ON 1ms OFF To turn off the LEDs briefly press and releas
20. may also be able to see a thin circular outline at the quartz liquid interface b Bubbles at near the quartz end appear as dark spots as small as 0 5mm in diameter or even as larger more defined spheres c Bubbles in the middle of the Light Guide may not be well defined spots but will appear as dark shadows d In extreme cases where the bubble is blocking the entire diameter of the Light Guide no light will come through even when pointing the distal end at a light source 10096 11 396 5 296 Appearance and output of Light Guides with bubbles relative to an LLG without bubbles 100 LUMEN DYNAMICS 69 035 00465R rev 5 X LCire Fluorescence Illumination In Control Can a bubbled Light Guide recover 12 5 12 Light Guides with small bubbles can sometimes recover Disconnect the Light Guide from the XLED1 and leave the Light Guide undisturbed on a shelf for 1 2 weeks at temperatures between 2 20 For this to be effective it is important to catch the bubble when it is small When should Light Guides be replaced 12 5 13 It is usually time to replace a Light Guide when a Dark or uneven areas become visible in the field of view a bubble is blocking part of the light b Asection of the Light Guide becomes noticeably warmer than the rest of the guide a bubble is blocking transmission of light forcing the Light Guide to absorb the energy Itis 2 3 years old OR has been in use for 4
21. on the computer screen Select Install Software Automatically Recommended click Next Once wizard finds software click on Finish Follow instructions for GUI Installation NDO U EON Note If window pops up with search on line for driver click yes 3 Alternative Installation Option Use MS Windows update as per your computer operating system Look for 1 Lumen Dynamics Hardware XLED1 2 Follow download instructions 3 Repeat for 274 Driver 1 install is USB Base Driver 274 install is Virtual Serial com Port 4 Locate downloaded Drivers in your Device Manager LUMEN DYNAMICS 18 035 00465R rev 5 X LCire Fluorescence Illumination In Control 5 Follow instructions for GUI Installation Note Ensure Installation of USB Drivers has been completed before installing the GUI 4 Installation of GUI Control Panel from CD 1 Insert the CD into your CD ROM drive 2 The USB Driver must be installed first Refer to paragraphs 1 2 or 3 in this section 3 Click on setup 4 Follow the Welcome Wizard download instructions Note A Software Development Kit SDK 035 00469R is available upon request This SDK will permit computer control of functions described in the serial command sets Please contact LDGI to request this document refer to section 20 4 5 Light Guide Auto Detect 4 5 1 The system will not allow the enabling of LED sources if there is not a Light Guide present in th
22. than this radius will result in permanent damage to the Light Guide 12 5 8 Make sure the Light Guide is cooled properly during use and prevent overheating a Always fully insert the Light Guide into XLED1 this ensures contact with a heat sink to conduct heat away from the Light Guide b Never obstruct the air vents on XLED1 Vents are located at the rear and underside of the unit c Do not remove the rubber feet on XLED1 or otherwise reduce block the space between the bottom of the unit and bench top This may compromise airflow through the unit d Ensure that the air being used to ventilate XLED1 is approximately room temperature e g do not place the X Cite unit on top of another heat producing instrument e lf a heated environmental chamber or incubator is being used for live cell imaging make sure that XLED1 and the Light Guide are located outside of the chamber or incubator 12 5 4 Do not expose the Light Guide to extreme temperatures above 35 C below 5 C for extended periods of time during use transport or storage this may cause degradation of the seals and allow air bubbles to form in the liquid 12 5 5 Never kink bend crush or stretch the Light Guide this type of mechanical stress may cause bubbles to form in the liquid and or damage to the outer sheath a Always allow adequate clearance between the Light Guide port of XLED1 and other objects to prevent excessive bending b Place XLED1 close eno
23. the Dichroic optical window with fingers microscope immersion oil or any abrasive object Refer to section 12 3 for handling and cleaning instructions Note the orientation of the Dichroic when removing there is a notched cut out on the top plate which allows the Dichroic s orientation key to pass through subsequently preventing the Dichroic from being replaced incorrectly Refer to Figure 33 11 1 4 Replace the Dichroic by inserting through the slot and lightly press down until a positive force is felt Hand tighten the locking screw do not use mechanical tooling such as screw drivers pliers etc 11 1 5 If the Dichroic is inserted incorrectly the module s orientation key located on the module frame will not pass through the cut out on the top plate resulting in an unseated Dichroic 11 1 6 Replace the XLED1 housing ensuring a snug fit Using the locking hub key supplied rotate the green locking hubs to the locked position Return the locking hub key to the locking hub key hanger located at the rear of the system The system will not allow the main power switch to function if the housing is not replaced properly 11 1 7 When changing a Dichroic the Edge value must be manually entered into the system Refer to section 7 7 to change the Edge value touch on the Dichroic to see the pop up window touch the top tab of the window and an edit screen will appear Type in the new Edge value and press GO to return to the maintenance screen Th
24. the reinforced strain relief portion of the Light Guide i LUMEN DYNAMICS 19 035 00465R rev 5 X Cite Fluorescence Illumination In Control LIGHT GUIDE PORT Figure 10 Light Guide Port 4 6 5 During use when the Light Guide is fully inserted the Light Guide LED indicator will illuminate green indicating full Light Guide insertion If the Light Guide is not fully inserted when the unit is powered up the Light Guide indicator LED will be RED there will be 3 beeps from the optional Touch Screen Controller The error icon will illuminate on the GUI Control Panel or on the Touch Screen Controller 4 6 6 To remove the Light Guide firmly grip the strain relief near the Light Guide retainer and pull out firmly Note XLED1 is designed for use with a 3mm liquid Light Guide Lumen Dynamics cannot guarantee the performance of the system if using Light Guides other than those supplied by Lumen Dynamics A i Note Refer to section 12 5 for tips to prevent premature degradation of Light Guides LUMEN DYNAMICS 2 035 00465R rev 5 Fluorescence Illumination In Control 4 7 Installing the Collimating Adapter 4 71 Ensure that the main power is off on the XLED1 unit 4 7 2 existing lamp housing must be removed from the microscope before the Collimating Adapter can be mounted Do not discard the existing mounting hardware since it will be used to secure the Collimating Adapter to the m
25. will be fully operational in any daylight level other than direct incident sunlight 5 1 1 Ensure that the Light Guide has been properly installed as per instructions in section 4 6 If the Light Guide is not fully inserted the Light Guide indicator LED will be RED there will be 3 beeps from the optional Touch Screen Controller The error icon will illuminate on the GUI Control Panel or on the Touch Screen Controller 5 1 2 Ensure the master power swtich is OFF Plug the XLED1 power adaptor into a properly grounded electrical outlet 5 1 8 Push the master power switch to ON and ensure that the internal fans are operational The cooling fans will remain functional throughout the complete start up to power down cycle 5 1 4 The LED ON indicator light will be off No Illumination 5 1 5 Open the GUI Control Panel at this time or if the optional Touch Screen Controller is connected wait for the XLED1 logon screen Note Do not use both the GUI Control Panel and the Touch Screen Controller E at the same time Do not connect the XLED1 Touch Screen Controller when the XLED1 is 5 1 6 system will commence with a Power On Self Test POST All outputs will be inactive and all inputs will be unresponsive during the POST cycle In the event of a POST error the system will report the error and either allow the user to proceed or halt depending on the error condition Refer to section 7 8 for alarm information if usi
26. 000 6000 hours 13 Troubleshooting Symptom Possible Cause CAUTION Service to be completed by qualified technical personnel only If the fails to POWER up or function properly use the following checklist to eliminate the most common causes of problems Check for the following 1 The power supply cord is securely connected to a grounded earthed mains socket functional 3 pin outlet 2 The power supply cord is securely connected into the 3 pin outlet on the rear of the controller unit 3 Ensure the housing is installed correctly e LUMEN DYNAMICS 7 035 00465R rev 5 X LCire Fluorescence Illumination In Control If the light intensity is too low check for the following 1 Ensure Power is off Verify that the emitting end of the Light Guide has been properly cleaned 2 Verify that the intensity level of the LED Modules are set correctly GUI Control Panel Main LED Screen or Touch Screen Controller Main LED screen and the LEDs have been enabled and triggers are off 3 Ensure Dichroic Modules are installed correctly 4 Check the Dichroic Edge value matches the actual value printed on the Dichroic a Turn off system ensuring to properly log out b Open the housing and remove each Dichroic module one at a time taking note of the Dichroic Edge value printed on the Dichroic module c Install the housing d Start up system and navigate to the maintenance screen Select each Dichroic
27. D Module and Driver male pins with the female socket connection and gently push down into place Tighten the Philips Head screws until a positive force is felt DO NOT OVER TIGHTEN THE SCREWS If using a calibrated torque meter Lumen Dynamics recommends a torque value of 5 7 Ib in t 0 3 Ib in 10 1 6 Replace the Dichroic refer to section 11 10 1 7 If there is a mismatch installation of either standard powered or high powered Drivers the corresponding LED will not operate 10 1 8 Replace the XLED1 housing ensuring a snug fit Insert the locking hubs and using locking hub key supplied rotate the green locking hubs to the locked position Return the locking hub key to the locking hub key hanger located at the rear of the system The system will not allow the main power switch to function if the unit cover is not replaced properly Note For assistance in determining the appropriate positions for Modules and Dichroics visit http www ldgi xcite com products xled1 configurator php e LUMEN DYNAMICS 60 035 00465 5 x lt Cite Fluorescence Illumination In Control Figure 32 LED Module and Driver Replacement LUMEN DYNAMICS 6 1 035 00465R rev 5 X Cite Fluorescence Illumination In Control 11 Dichroic Replacement CAUTION To protect the LED Module Driver and system from electrostatic discharge ESD related damage which can disable the system cause latent defects or perm
28. D module into the XLED1 system Note Utmost care must be taken to not contaminate the lens or the Excitation filter e g finger prints dust etc The use of gloves or other material suitable for handling optics is recommended LUMEN DYNAMICS 1 7 035 00465R rev 5 Fluorescence Illumination In Control 4 4 Graphical User Interface GUI Note Ensure user is logged in as Administrator The USB drivers and GUI can be downloaded from our website under the SOFTWARE DOWNLOADS section of the Resource Centre http www ldgi xcite com support php System Requirements Operating System Windows XP 32 64bit Windows Vista 32 64bit Windows 7 32 64bit Windows 2003 Server 32 64bit Windows 2008 Server 32 64bit Windows 2008 R2 Minimum Hard Drive Space 5MB 1 Installation of USB Drivers from CD Note This procedure will install the USB and virtual COM port driver 1 Insert the CD into your CD ROM drive 2 Connect the XLED1 to the computer s USB port using the supplied USB cable and turn on the XLED1 3 The XLED1 Drivers will be detected select automatic installation 4 Upon completion of driver installation follow instructions for GUI Installation remove the CD 2 Installation of USB Drivers from web windows update Log on to internet Connect USB cable from XLED1 to computer Turn on XLED1 Found New Hardware will appear on the computer screen Found New Hardware Wizard will appear
29. E n ERE ER MESA 72 15 Environmental Conditions rh CH cu ER FREE EN ERER EK EN ERER 74 15 Regulatory Compliance 74 17 Warranty 78 18 Warranty Tea Ol e 79 19 80 reir ndis c 81 Figures Figure 1 XLEDT Front 8 Figure 2 XLEDT Back Panel Figure 3 Touch Screen Controller ererri tran rta rna nann Pan ur x RR RR ERN RES EE ERR RR MNA MERE PE IEEK ERE Figure 4 LED Module Driver and Dichroic Locations Figure 5 Locking T M Figure 5 Dichroic Replacement sssisicicsisccenescccccnccceseceancewscescdenctencanwedensaviecescannidencepneanecccnnepeseeneces Figure 7 Typical Figure 8 Inserting filter into filter Figure 9 Attaching filter assembly to module esee Figure 10 Light Guide Port e Figure 11 Collimating Figure 12 Main LED Screen
30. Locking Hub Key supplied Torque tool not supplied LED Modules Drivers and Dichroics are shipped in protective ESD bags LED Modules are shipped with a protective lens cap covering the LED lens 10 1 1 Ensure the system is OFF and the AC power cord is unplugged from the power source Allow system to cool for 5 minutes before removing the housing 10 1 2 Using the locking hub key supplied rotate the green locking hubs 90 degrees and remove the locking hubs Holding the XLED1 housing at the back slide the XLED1 housing up and back away from the fascia for removal The locking hub key can be stored on the locking hub key hanger located on the back panel of XLED1 i Figure 31 Locking Hub LUMEN DYNAMICS 59 035 00465R rev 5 X Cite Fluorescence Illumination In Control 10 1 3 Replacement LED Modules are shipped with their unique Drivers Both components must be replaced at the same time Refer to Figure 32 Note Remove the corresponding Dichroic before removing the LED module and driver to prevent accidental contact with the Dichroic Refer to section 11 10 1 4 To remove the LED Module and Driver unscrew the Philips Head screws and remove Module and Driver Caution Do not touch the LED lens surface Skin oils and scratches can cause the optical output to decrease 10 1 5 Firstly remove the protective LED lens cap if present on the new LED module To replace the LED Module and Driver align the LE
31. OIC WINDOW ORIENTATION CUT OUT DICHROIC MODULE 219 A Figure 6 Dichroic Replacement LUMEN DYNAMICS 1 5 035 00465R rev 5 X LCire Fluorescence Illumination In Control 4 3 Installing Excitation Filter Holders All XLED1 units include 4 filter holders that accommodate any standard 25mm band pass filter Additional filter holders can also be ordered separately by using part number 019 00175R 4 3 1 4 3 2 4 3 3 4 3 4 Figure 7 Typical XLED1 LED Module Follow instructions as per section 4 2 of the XLED1 user manual to open the XLED1 system and remove the LED module of interest Only remove the LED module there is no need to remove the driver NOTE care must be taken to not contaminate or damage the lens or excitation filter wearing gloves is recommended when performing this procedure Place the filter holder on a flat clean surface as shown below With the arrow indicating the coated side of the filter pointing upward place the filter inside the filter holder Figure 8 and tighten the set screw with supplied allen key Figure 8 Inserting filter into filter holder LUMEN DYNAMICS 1 6 035 00465R rev 5 X LCire Fluorescence Illumination In Control 4 8 5 Attach the excitation filter filter holder into the LED module lens as depicted below and secure the set screw with the provided allen key Figure 9 Attaching filter assembly to module 4 36 Re insert the LE
32. SCREEN Figure 19 Alarm Code Screen GUI Error Pop up Screens Descriptions Error Window Error Description Screen Location Light Guide not inserted Request to turn on LED could not be completed due to the Light Guide not being fully inserted All screens Enter Delay or Off Time Request to start Pulse Generator cycle could not be completed due to missing delay or off time entry Trigger screen To change units or trigger LED must be off Requested settings change was not completed as the LED was on Trigger screen Alarm Active on LED Proceed to maintenance screen Request to turn on an LED could not be completed due to an active alarm Main screen Dichroic not found Wrong Dichroic number entered Maintenance screen Function only available on XLEDCP Pop up error window appears when trying to perform a function which is not available via the GUI Pop up error window on all Screens Table 3 Error Pop up Screen Descriptions GUI e LUMEN DYNAMICS SU 035 00465R rev 5 X Cite Fluorescence Illumination In Control 6 6 1 8 visual alarm indicators on the Alarm Code Screen refer to Figure 19 are indicators only and do not function as alarm clearing buttons pads 6 6 2 The LED System is designed to detect certain conditions that may affect safe or proper functioning These conditions are categorized as one of two alar
33. UMEN DYNAMICS 44 035 00465R rev 5 X Cite Fluorescence Illumination In Control 7 6 Trigger Graphs Screen es Touchscreen Locked Light Guide Inserted Alarm ia RETURN TO TRIGGER SCREEN Figure 25 Trigger Graphs Screen Touch Screen Controller 7 6 1 trigger graph screen shows all 4 LED Modules as separate graphical representations of the triggering patterns assigned from the Set LED Trigger screen 7 6 2 To return to the LED Trigger Screen touch Return to Trigger Screen LUMEN DYNAMICS 45 035 00465R rev 5 X Cite Fluorescence Illumination In Control 7T 7 Maintenance Screen DRIVERS LED MODULES CHANGE PIN A 620 700nm A FWHM 635 10nm Type High Power _ Current 24 C IE ALARM Cutoff at 75 C LED used 4 hrs DICHROICS SN 4 Mfg 7 10 2012 SYSTEM RETURN TO LED SCREEN TRIGGER Figure 26 Maintenance Screen Touch Screen Controller 7 7 1 maintenance screen gives an overview of the locations of the 4 individual LED Modules Drivers and Dichroics 7 7 2 Touch one of the green lit Modules to retrieve the pop up window showing the selected Modules present attributes e X Displays LED Module wavelength range in nm J FWHM Full Width Half Max e g depicts LED peak wavelength FWHM the useful bandwidth of the LED e Identifies the LED Module as either Standard Power or High Power version whic
34. UMEN DYNAMICS 5 1 035 00465R rev 5 X Cite Fluorescence Illumination In Control gt The screen will go blank after timeout lapses Touch the screen anywhere to return to present screen POWER ON SELF TEST gt Press to start the Power On Self Test The system will commence with a Power On Self Test POST All outputs will be inactive and all inputs will be unresponsive during the POST cycle In the event of a POST error the system will report the error and either allow the user to proceed or halt depending on the error condition Refer to section 7 8 for error codes ALIGN TOUCH SCREEN gt Press this pad to calibrate the touch screen accuracy gt Upon pressing the pad follow the instructions on the screen there is a timeout of 10 seconds between each test If time lapses the Touch Screen Controller will return to the System Information Screen Press this pad again to calibrate Error Window Error Description LED wavelength over temperature LED Module is above allowable working temperature LED Module is off when system is told it is on only valid LED wavelength current off alarm when not in trigger mode LED wavelength under temperature LED Module is below allowable working temperature LED Module is on when system is told it is off only valid LED wavelength current on alarm when NOT in trigger mode LED lt wavelength gt NVM error LED Module memory
35. YNAMICS 5 5 035 00465 5 X LCire Fluorescence Illumination In Control Table 8 External Trigger Pin Assignments PIN Signal name Description Location Port 1 trigger input High LED on 1 Port 1 trigger Low LED off Port 1 red BNC cable Port 2 trigger input High LED 2 Port 2 trigger Low LED off Port 2 green BNC cable Port 3 trigger input High LED 3 Port 3 trigger Low LED off Port 3 blue BNC cable 4 n c Not Connected 5 n c Not Connected 6 Port 1 trigger Ground Port 1 red BNC cable 7 Port 2 trigger Ground Port 2 green BNC cable 8 Port 3 trigger Ground Port 3 blue BNC cable 9 n c Not Connected 10 n c Not Connected 11 Port 4 Global trigger Ground Port 4 white black BNC cable 12 n c Not Connected Port 4 trigger input High LED 13 Port 4 trigger Low LED off Port 4 white BNC cable Global trigger input High LED on 14 Global trigger Low LED off Global black BNC cable 15 n c Not Connected Table 9 External Sync Out Pin Assignments OUTPUT 5 5V 8mA max PIN Signal name Description Location 1 Port 1 sync Port 1 Sync out a LED on Low LED Port 1 red BNC cable 2 Port 2 sync Porta Syne gut green BNC cable 3 Port 3 sync Port 3 Sync out 5 Low LED Port 3 blue BNC cable 4 n c Not C
36. act Lumen Dynamics Group Inc 1 This product has been tested to the requirements of CAN CSA C22 2 61010 1 second edition including amendment 1 or a later version of the same standard incorporating the same level of testing requirements ATTENTION Electro Static Discharge ESD Observe precautions for handling Electrostatic Sensitive Devices LUMEN DYNAMICS 2 035 00465R rev 5 Fluorescence Illumination In Control 2 2 gt gt gt Safety Precautions This series of cautions and warnings relate to the operation and maintenance of the X Cite XLED1 System They are also presented throughout this User s Guide where necessary RISK GROUP 2 CAUTION Possibly hazardous optical radiation UV emitted from this product Use appropriate shielding IEC 62471 2 2009 WARNING Visible and invisible radiation is emitted from the aperture of the Light Guide avoid exposure to beam Never look into the light emitting end of the Light Guide The light could severely damage the cornea and retina of the eye if the light is observed directly Eye shielding and protective clothing must be used at all times to protect exposed skin CAUTION Use of the XLED1 system is intended only for microscopes using Lumen Dynamics collimating adaptors Any attempt to use this system with a microscope not approved by LDGI is not recommended and may impair system safety WARNING Always make sure the Light Guid
37. anent damage or require repair by service technicians please take the following ESD prevention steps e Ensure the unit is unplugged Ensure you are properly grounded ideally by using an ESD grounding strap attached to an ESD protective surface Refer to section 2 3 for principles of ESD and additional ESD precautions Note Ensure the users log out of the Touch Screen Controller before powering down or all entered data from the latest session will not be saved refer to section 7 3 system is defaulted NOT to save data when using the system logged in as Guest regardless of logging out or not A Tools Required Locking Hub Key supplied DICHROIC B B Caution The Dichroic optical window operational life can be significantly shortened if it is handled incorrectly Do not touch the window surface Skin oils and scratches can cause the window to fail prematurely Refer to section 12 3 for handling and cleaning instructions 11 1 1 Ensure the system is OFF and the AC power cord is unplugged from the power source 11 1 2 Using the locking hub key supplied rotate the green locking hubs and remove the XLED1 housing refer to section 10 1 2 Note When replacing the Dichroic remove and replace one at a time LUMEN DYNAMICS 62 035 00465R rev 5 Fluorescence Illumination In Control 11 1 3 Remove the plastic locking screw and gently pull out the module avoid touching
38. ange the Dichroic will transmit specified in nm 6 4 4 When changing a Dichroic the Edge value must be entered into the system To change the Edge value click on the Dichroic icon to see the pop up window click on the top tab of the Dichroic information window and a pop up Dichroic Selection screen will appear Select the new edge value from the drop down menu and click OK to return to the maintenance screen The Reflect and Transmit values will be automatically populated 6 4 5 If the new Dichroic is not listed in the drop down menu refer to Section 6 5 to obtain the Edge Wavelength Low Reflect High Reflect Low Pass and High Pass numbers LUMEN DYNAMICS 3 2 035 00465R rev 5 X Cite Fluorescence Illumination In Control W cna x 21 Control Panel LEDon n Locked Light Guicle Inserted CHANGE PIN Reflect 327nm 490nm Transmit 500nm 750nm P Dichroic Da abase Edge WaveLength Low Reflect High Reflect Low Passive High Passive SYSTE 1 409 344 404 415 570 2 495 327 490 500 750 580 495 570 590 650 RETURN TOLE 350 596 750 612 350 380 0 Update Dichroic Database T Figure 18 Dichroic Database GUI 6 4 6 Click on the green X Cite Icon located on the top left corner of the maintenanc
39. ceases to be valid This warranty may not form the basis for any claims for damages in particular not for compensation of consequential damages This warranty is not transferable No warranty is extended to perishable items if purchased separately or included in systems These may include but are not limited to fuses air filters cables Light Guides light lines and Light Guide adapters Refer to Table 11 System Components Warranty Note Removal or interfering of the tamperproof label located on the underside the XLED 1 will result in Void Warranty e LUMEN DYNAMICS 78 035 00465 5 X LCire Fluorescence Illumination In Control 18 Warranty Table System Component Warranty void if e The unit has been subjected to misuse or 1 year mishandling e The unit has been opened or tampered with X Cite XLED1 Main System and Drivers e The unit has been subjected to misuse or mishandling e The unit has been opened or tampered with LCD display damage physical Cable damage due to mishandling X Cite XLED1 Optional Touch Screen Controller HD 15 pin connector damage Lens damage due to misuse or mishandling E Cable damage due to misuse or mishandling All LED Modules whichever e Module has been opened or tampered with comes e The LED Drivers are not included 1 year warranty first applies here e The Dichroics are not included 1 year warranty applies here Table 11 System Com
40. ch LED module section has a coarse intensity adjustment slider and fine intensity adjustment UP A and DOWN v pads The fine intensity adjustment arrows allow intensity adjustments from 5 to 100 in 0 196 increments A numerical value can be entered by tapping on the 96 value which opens up a numerical keypad window 7 3 4 The main screen has a global LOCK ALL LEDS this button sets all the individual lock buttons to locked 7 3 5 main screen has a global TURN OFF ON ALL LEDS pad which will disable and enable all LEDs at once e LUMEN DYNAMICS 4 0 035 00465 5 Fluorescence Illumination In Control 7 3 6 main screen displays icons for Touch Screen Locked Light Guide Inserted Alarms and LED 7 3 7 Itis very important to use the LOGOUT touch pad before powering down the system Failure to logout before powering down will result in loss of the present settings the system will then default to previous settings upon the next start up 7 3 8 Ability to navigate to the TRIGGER screen and the MAINTENANCE screen A Note Ensure that users log out of the Touch Screen Controller before powering down or entered data from the latest session will not be saved refer to section 7 3 The system is defaulted NOT to save any data when logged in as Guest regardless of logging out or not 7 4 Edit LED Name Screen Touchscreen Loc
41. d in a residential area If this equipment does cause harmful interference to radio or television reception which can be determined by turning the equipment off and on the user is encouraged to try to correct the interference by one or more of the following measures e Reorient or relocate the receiving antenna e Increase the separation between the equipment and receiver WARNING Changes or modifications not expressly approved by Lumen Dynamics could void the user s authority to operate the equipment Use only the shielded power supply cord supplied with the XLED1 System LUMEN DYNAMICS 75 035 00465 5 X LCire Fluorescence Illumination In Control CE Marking AS per Council Directives 2006 95 EC Low Voltage Directive 2004 108 EC EMC Directive 2002 96 EC WEEE Directive 2011 65 EU RoHS Directive WEEE Directive 2002 96 EU A The symbol above indicates that this product should not be disposed of along with municipal waste that the product should be collected separately and that a separate collection system exists for all products that contain this symbol within member states of the European Union e The equipment that you bought has required the extraction and use of natural resources for its production It may contain hazardous substances that could impact health and the environment e In order to avoid the dissemination of those substances in our environment and to diminish the pressure on the nat
42. e trei HR He e ce ens XLED1 Locking Hub eee Locking Hub Key Hanger refer to Figure 2 for install location CD w Manuals GUI USB Driver eese Product Registration Card sse XLED1 Excitation filter holders set of 4 XLED1 trigger cable Please go online to register at http www ldgi xcite com registermyproduct Refer to Table 12 for individual part numbers f your packaged system is missing any of the listed components call Lumen Dynamics at 905 821 2600 or Toll Free 1 800 668 8752 Refer to the website for all LED module driver and Dichroic locations and combinations http www ldgi xcite com products led modules php LUMEN DYNAMICS 7 035 00465R rev 5 X Cite Fluorescence Illumination In Control 3 1 XLED1 Front Panel LIGHT GUIDE INDICATOR LIGHT GUIDE PORT HOUSING LOCKING HUBS BOTH SIDES EXPANSION Port NOT FOR USE MASTER POWER ON OFF LED ON OFF BUTTON LED SWITCH ON INDICATOR Figure 1 XLED1 Front Panel LED Indicator Status Description Green Light Guide fully inserted Light Guide LED 9 d Red Light Guide not fully inserted Green Enabled On LED ON No Illumination Disabled Off Table 1 Front Panel LED Indicators LUMEN DYNAMICS 8 035 00465R rev 5 X LCire
43. e screen and click on Dichroic Database A Dichroic Database pop up window will appear Fill in the number obtained from the list below Section 6 5 click Update Dichroic Database to save new entries Refer to Figure 18 LUMEN DYNAMICS 3 3 035 00465R rev 5 X Cite Fluorescence Illumination In Control 6 5 Dichroic Values 409 325 404 415 530 458 350 450 467 950 495 350 488 502 750 538 350 528 547 950 605 350 596 612 750 380 350 375 385 450 416 360 407 425 575 660 350 654 669 750 6 6 Alarm Information Alarms Description Severity Recoverable Max LED module Max Temp Exceeded Over Temperature Critical Yes Protection Min LED module Min Temp Exceeded Under Temperature Major Yes Protection LED Module Fail LED Module Failure Critical No Eight Guide NGI Light Guide Removed Major Yes Present Internal Comms Error Communication error Critical No Watchdog Timeout Watchdog Tripped Critical No NVM Fail System Performance System Performance Major Yes Table 2 Alarm Codes GUI LUMEN DYNAMICS 34 035 00465R rev 5 X Cite Fluorescence Illumination In Control KJ XLED1 Control Panel TouchScreen Locked MB Light Guide Inserted Alarm Min Temp Exceeded NVM Fail Max Temp Exceeded LED Module Fail Light Guide Not Present Comms Error Watchdog Timeout System Performance RETURN TO MAINTENANCE
44. e Edge value is printed on the Dichroic 11 1 8 Reflect and Transmit values in the pop up window will be automatically populated if using the GUI Refer to section 6 4 LOCKING SCREW ORIENTATION KEY DICHROIC OPTICAL WINDOW ORIENTATION CUT OUT Figure 33 Dichroic Replacement LUMEN DYNAMICS 035 00465R rev 5 Fluorescence Illumination In Control 12 Routine Care and Maintenance To protect the system from electrostatic discharge ESD related damage which can disable the system cause latent defects or permanent damage or require repair by service technicians please take the following ESD prevention steps e Ensure the unit is unplugged e Ensure you are properly grounded ideally by using an ESD grounding strap attached to an ESD protective surface Refer to section 2 3 for principles of ESD and additional ESD precautions A Caution Routine maintenance should only be completed by qualified technical personnel to avoid risk of injury to the end user A Warning Use UV eye and skin protection during servicing if access to the UV LED Modules is required during system operation 12 1 General 12 1 1 Operate the unit in a well ventilated area with at least 50mm clearance on all sides to allow adequate space for the air vents located on the bottom of the unit 12 1 2 Always connect the XLED1 external power module AC supply cord to a grounded outlet earthed mains only 12 1 3 When nec
45. e is properly inserted into the XLED1 and into the microscope prior to turning on power to the unit This will minimize the risk of exposure to UV light CAUTION Removal of the service cover is intended for qualified technical personnel only No user serviceable parts are located within the XLED1 chassis or the Touch Screen Controller This system contains hazardous voltages do not attempt to remove access covers while equipment is energized WARNING To reduce the risk of fire or shock always replace the AC mains fuses with the same type and rating F6 3A 250V Ensure that the X Cite XLED1 System external power supply cord is connected to a grounded earthed mains socket 3 pin outlet only Use only the power supply cord set provided with the system Substitution is not recommended operation and or performance may be affected and may impair operating safety WARNING The Level of UV and visible energy supplied by the X Cite XLED1 System is sufficient to ignite flammable substances During installation in an automated environment the appropriate measures must be included to prevent any such occurrence e LUMEN DYNAMICS 3 035 00465R rev 5 X Cite Fluorescence Illumination In Control WARNING Should the X Cite XLED1 System be used in a manner not specified by Lumen Dynamics the protection provided by the equipment may be impaired Do not attempt to connect the Touch Screen Controller to any type of electrical equipm
46. e on the lens surface Refrain from touching the optical window with any foreign object which could scratch the optical window A Caution Do not touch the LED lens surface Skin oils and scratches can cause the optical output to decrease Cleaning Procedure for LED Lens Assembly e Improper handling and cleaning practices can damage lens surface which are utilized in this assembly Proper handling and cleaning technique should be adhered to Below are the recommended procedures for cleaning of the lens assembly Handling e Always handle the lens assembly by the metal frame never touch the lens surface with your fingertips The moisture or oils on your fingertips can damage the lens surface Fingerprints left on a lens surface for an extended period of time can become a permanent stain Even if you are wearing gloves avoid touching the lens surface Never handle the lens assembly with metal implements or tweezers e To store lens assemblies wrap them individually a clean ESD safe bag and store in a safe dry place e Never store multiple unwrapped lens assemblies in the same bag as they may become damaged if they come in contact with each other LUMEN DYNAMICS 65 035 00465R rev 5 X LCire Fluorescence Illumination In Control Cleaning Materials The following materials are recommended for cleaning the lens assembly e Rubber Bulb Dust Blower non pressurized e Lint free lens tissue lint free cotton swabs li
47. e receptacle 4 5 2 The system will disable all LED sources if the Light Guide has been detected as removed from the unit Refer to section 4 6 for Light Guide installation 4 5 8 lightguide LED on the front panel of XLED1 will illuminate green indicating the Light Guide insertion is correct If the Light Guide is not fully inserted the Light Guide indicator LED will illuminate RED there will be 3 beeps from the optional Touch Screen Controller The error icon will illuminate on the GUI Control Panel or on the Touch Screen Controller 4 5 4 The system will not allow the main power switch to function if the housing is not replaced properly When replacing the XLED1 service housing ensuring a snug fit and using locking hub key supplied rotate the green locking hubs to the locked position 4 6 Inserting Removing Light Guide 4 6 1 Ensure that the main power is off on the XLED1 unit 4 6 Ensure the power cord is not connected to XLED1 4 6 8 Ensure that the protective end caps are removed from both the input and output ends of the Light Guide prior to installation and remove plastic plug if present from the Light Guide port on the front panel of XLED1 4 6 4 Hold the Light Guide by the grey sleeve strain relief portion only insert the Light Guide into the Light Guide port Push the Light Guide in until it seats with a positive click Note Never grip the Light Guide during installation or removal a place other 1 than
48. e the LED ON OFF button NOTE The Live Cell Mode is essentially an internal trigger and the control panel will appear as though a trigger is ON Choosing the LEDs that are enabled along with their intensities can be done through the touch screen controller or the GUI To ensure that an LED is disabled upon pressing the LIVE CELL MODE button on the front of the unit the intensity of the LED must be set to zero on the slider in the control panel If LEDs are turned on through the controller or GUI only those LEDs that are ON will pulse at the default duty cycle of 1ms at the intensity set on the LED slider To disable Live Cell Mode the front panel LED ON OFF button must be depressed until Light Guide indicator light turns green hold on off button down for at least 5 seconds LIGHT GUIDE INDICATOR LUMEN DYNAMICS 5 3 035 00465 5 X Cite Fluorescence Illumination In Control 8 2 Changing duty cycle in Live Cell Mode 8 2 1 duty cycle may be changed using the XLED1 touch screen controller OR the GUI but NOT both and NOT while the LEDs are on 8 22 Trigger MUST be stopped in the TRIGGER control screen before changing duty cycle 8 2 8 Using XLED1 touch screen controller 8 2 3 1 Once triggers are stopped make the change required to the pulse duration by adjusting ON time and OFF time 8 2 3 2 To save these setting for future use the user must use the LOG OUT button 8 2 3 3 When you log in
49. e trigger designation to OFF Internal indicated by time units External and Global Press the CHANGE TRIGGER pad to select a trigger designation Each LED module can only have one trigger designation 6 2 3 The triggers are described below LUMEN DYNAMICS 2 7 035 00465R rev 5 Fluorescence Illumination In Control A The default trigger designation is set to OFF in order to allow control of the system from the main LED screen B When in high speed triggering mode the sync signal is an acknowledgement of a received command C When the trigger is set to External the system will accept an external signal control to the selected LEDs refer to Table 8 D When the trigger is set to GLOBAL the system will accept a trigger signal through a single pin to control all LEDs that are set to GLOBAL refer to Table 8 Note When selecting External or Global trigger the LEDs will not turn on until a trigger signal is present The LEDs will accumulate hours while in External or Global trigger mode To prevent unnecessary hour accumulation LED modules while using the XLED1 system users s are advised to enable these XLED1 advanced triggering modes only during the window of time that will be used for such purposes Subsequent to using the advanced triggering capabilities of the XLED1 system the user should ensure that triggering is OFF the trigger configuration scree
50. ell Configuration units 8 1 Enabling Live Cell ee dice eei E sat d batte tp oan LUMEN DYNAMICS 1 035 00465 5 X LCire Fluorescence Illumination In Control 8 2 Changing duty cycle in Live Cell enne nennen 54 9 External 9 2 rr 55 9 1 External Trigger Interface E 55 9 2 Trigger Input Sync Output Signals 57 9 3 I O Signal Descriptions uiui etit dept dud bee 57 10 LED Module and Driver 59 MEE deditum 62 12 Routine Care and 64 12 1 dons 64 12 2 Cleaning Procedure XLED1 amp Touch Screen 64 12 3 Handling and Cleaning of the LED Lens 65 12 4 Handling and Cleaning of the Dichroic essere 66 12 5 Tips to Prevent Premature Degradation of Light 68 13 Troubleshooting Symptom Possible Cause 70 14 Technical Specifications SE
51. en Medical industrial grade 7inch TFT viewable diagonal Resistive touch screen with 16 9 aspect ratio and LED backlight with adjustable intensity Resolution 800 x 480 pixels Display Viewing Area 152 4mm W x 91 44mm H External Dimensions 197mm W x 136mm D x 63mm without legs extended Program Lumen Dynamics proprietary graphical user interface Connectivity 2 5m connectorized power and data combo cable Power 10 watts 5 2V max LED Intensity Control 5 100925 in 0 1 increments LUMEN DYNAMICS 7 3 035 00465R rev 5 X LCire Fluorescence Illumination In Control 15 Environmental Conditions Operating Conditions Ambient Temperature 5 C to 35 Altitude 2000m max Atmospheric Pressure 700 to 1060 hPa Relative Humidity 1596 to 8596 non condensing Installation Category II Pollution Degree 2 Transport and Storage Conditions Temperature 10 to 70 C Relative Humidity 10 to 90 Atmospheric Pressure 500 to 1060 hPa 16 Regulatory Compliance Product Safety IEC 61010 1 2001 Safety Requirements for Electrical Equipment for Measurement Control and Laboratory Use Part 1 EN 61010 1 2001 Safety Requirements for Electrical Equipment for Measurement Control and Laboratory Use CAN CSA C22 2 No 61010 1 04 UL 61010 1 2nd Edition Safety Requirements for Electrical Equipment for Measurement Control and Laboratory Use Part 1 General Requirements General Requirements IEC Eq
52. en les Touchscreen Locked Light Guide Inserted eal Alarm ea LED on Enter your User ID to access your personalized settings ENTER AS GUEST Figure 21 Log In Screen Touch Screen Controller LUMEN DYNAMICS 3 8 035 00465R rev 5 X Cite Fluorescence Illumination In Control 7 2 1 7 2 2 7 2 3 7 2 4 7 2 5 A 7 2 6 7 2 7 7 2 8 Maximum Users 5 User ID Numbers User ID numbers must be 4 digits The system ships with qty 5 default ID log in numbers 1234 2234 3234 4234 5234 Note User ID 5 settings will be default on XLED1 power up if the XLED1 Touch Screen Controller is disconnected during the next power up Enter the default ID 4 digit log in number to access the system The XLED1 will remain non functional if the incorrect user ID is entered Upon entering User ID press GO to navigate through all the Touch Screen Controller screens and control the XLED1 If logged in as a Guest all data will not be saved after completion of session Note Ensure that users log out of the Touch Screen Controller before powering down entered data from the latest session will not be saved refer to section 7 3 The system is defaulted NOT to save any data when logged in as Guest regardless of logging out or not If desired the user can create a personal PIN number Navigate to the Maintenance screen and press the CHANGE PIN pad Enter new PIN number
53. ent other than the XLED1 main system as indicated in Figure 2 of this manual A WARNING This unit is designed for bench top use only Always ensure that the unit is operated on a hard stable surface This will prevent obstruction of the bottom chassis ventilation openings Any obstruction of these openings could result in a possible over heating condition Do not attempt to remove or tamper with the rubber feet located on the bottom of the unit CAUTION Use of controls or adjustments or performing procedures other than those specified herein may result in hazardous radiation exposure emitting end of the Light Guide The presence of skin oils may result in a decrease in system performance Always ensure the system power is turned off prior to disconnecting or re connecting the Light Guide A CAUTION To prevent damage degradation of the Light Guide always allow adequate clearance at the front of the XLED1 unit to prevent kinking or excessive bending A The Light Guide operation can be affected if handled incorrectly Never touch the light WARNING Do not lift the XLED1 from the rear of the removable housing when system is either on or off CAUTION To protect the LED module driver and system from electrostatic related damage which can disable the system permanently damage the LED module amp driver or require repair by service technicians place all Modules on an antistatic surface such as an antistatic discharge mat an a
54. essary clean the aperture of the Light Guide using an optical cleaning solution Refer to section 12 5 12 2 Cleaning Procedure XLED1 amp Touch Screen Controller 12 2 1 Ensure the XLED1 master power switch is in the off position and the AC power cable is unplugged 12 22 Use a mild soap solution as the cleaning media 12 2 8 Dampen a clean lint free cloth tissue or swab with the mild soap solution and wipe the exterior of the housing Avoid excessive moisture when wiping in the air vent areas e LUMEN DYNAMICS 64 035 00465R 5 X Cite Fluorescence Illumination In Control 12 2 4 Using a new dampened tissue or pre saturated tissue clean the Touch Screen Controller display by gently wiping the surface with one continuous stroke in one direction A Note Never apply a dirty tissue or swab to the display of the Touch Screen Controller 12 2 5 Inspect the display to verify no residue is present Note Regular cleaning of the display is recommended for optimal performance CAUTION Do not use any alcohol based or acetone based solutions for cleaning the XLED1 or the Touch Screen Controller 12 3 Handling and Cleaning of the LED Lens Assembly Cleaning of the lens assembly is not generally required However if any visible contamination or finger prints appear in the lens surface the contaminants may cause reduced light output Light output degradation may also result from scratches digs and other damag
55. h is determined by the LED type e Current displays the current working temperature of the LED module in e Cutoff Temp indicates the maximum specified working temperature of the LED module in C e LED Used Records the current accumulated LED module illumination hours e Serial Number displays the serial number of the LED module e Mfg ON displays the manufacturing date of the LED module The LL touch pads are for navigating to the various screens e LUMEN DYNAMICS X Cite Fluorescence Illumination In Control es Touchscreen Locked Light Guide Inserted CHANGE PIN Reflect 327nm 490nm Transmit 500nm 750nm SYSTEM DICHROIC DATA WINDOW _ RETURN TO LED SCREEN TRIGGER Figure 27 Maintenance Screen Dichroic Data Touch Screen Controller 7 7 3 When touching a Dichroic icon a Dichroic pop up window will display the following attributes Edge specified in nm top tab of the window Reflect the wavelength range that the Dichroic will reflect specified in nm Transmit the wavelength range the Dichroic will transmit specified in nm 7 7 4 When changing a Dichroic the Edge value must be manually entered into the system To change the Edge value touch on the Dichroic icon to see the pop up window touch the top tab of the Dichroic information window and an edit screen will appear Type in the new Edge value and press GO to return to the maintenance screen Refer to secti
56. has been triggered A OV signal will be produced on Port 2 sync when LED Module 2 has not been triggered A 5V signal will be produced on Port 3 sync when LED Module 3 has been triggered A OV signal will be produced on Port 3 sync when LED Module 3 has not been triggered A 5V signal will be produced on Port 4 sync when LED Module 4 has been triggered A OV signal will be produced on Port 4 sync when LED Module 4 has not been triggered 58 035 00465R rev 5 e LUMEN DYNAMICS X Cite Fluorescence Illumination In Control 10 LED Module and Driver Replacement CAUTION To protect the LED Module Driver and system from electrostatic discharge ESD related damage which can disable the system cause latent defects or permanent damage or require repair by service technicians please take the following ESD prevention steps e Ensure the unit is unplugged e Ensure you are properly grounded ideally by using an ESD grounding strap attached to an ESD protective surface Refer to section 2 3 for principles of ESD and additional ESD precautions Note Ensure that users log out of the Touch Screen Controller before powering down or all entered data from the latest session will not be saved refer to section 7 3 The system is defaulted NOT to save any data when using the system logged in as Guest regardless of logging out or not 2 Philips Head Screwdriver not supplied Tools Required
57. i xcite com products led modules php The X Cite XLED1 System joins the Lumen Dynamics family of light systems offering the same high level of innovation quality and reliability that our customers have come to expect Since 1982 Lumen Dynamics has combined next generation optical engineering state of the art electronics and fibre optics to produce sophisticated technologies that employ light Today Lumen Dynamics is a leading developer of light based systems for industries ranging from manufacturing to bio imaging and we are unmatched in our commitment to quality and service Lumen Dynamics recommends reading this manual to discover all the features available for the X Cite XLED1 System Please ensure that you register your XLED1 at http www ldgi xcite com registermyproduct X Cite XLED1 Enclosure containing all opto mechanical assemblies control electronics and port interfacing Touch Screen Controller optional Separate remote enclosure with touch screen display module for control of the XLED1 e LUMEN DYNAMICS 1 035 00465R rev 5 X Cite Fluorescence Illumination In Control 2 Safety 2 1 Glossary of Symbols Caution risk of danger consult accompanying documents Input Output Signals 6 Input Signal gt Output Signal RISK GROUP 2 CAUTION Possibly hazardous optical radiation UV emitted from this product Use appropriate shielding IEC 62471 2 2009 For detailed information cont
58. ically only 12 sub pulses can be generated if more are needed try using different time domains to achieve the desired results 2 sec 2 sec 2 sec User Specified _ LED A Period 6 seconds System Addition 1 sec 2 sec 1 sec 2 sec ED B Period 4 seconds LED B ext 2 sec gt LEDC Period 2 seconds repeated 3 times e LUMEN DYNAMICS 29 035 00465 5 X Cite Fluorescence Illumination In Control 6 3 Trigger Graphs Screen as Touchscreen Locked Light Guide Inserted 5 Alarm RETURN TO TRIGGER SCREEN Figure 14 Trigger Graphs Screen GUI 6 3 1 The trigger graph screen shows all 4 LED Modules as separate graphical representations of the triggering patterns assigned from the Set LED Trigger screen 6 3 2 To return to the LED Trigger Screen touch Return to Trigger Screen e LUMEN DYNAMICS 3 035 00465R rev 5 X Cite Fluorescence Illumination In Control 6 4 Maintenance Screen DRIVERS LED MODULES Light Guide Inserted Alarm CHANGE PIN 620 700nm A FWHM 35 10nm Type High Power _ Current 24 IE ALARM Cutoff at 75 C LED used 4 hrs DICHROICS SN 4 Mfg 7 10 2012 SYSTEM RETURN TO LED SCREEN TRIGGER Figure 15 Maintenance Screen GUI 6 4 1 The Maintenance screen gives an overview of the location of the 4 individual LED modules dr
59. icroscope 4 7 3 Remove the Collimating Adapter from its packaging see 5 Note The Collimating Adapter has been set at the factory No adjustments the customer are required however some adapter models are outfitted with centering set screws for longer light path applications Separate instructions are included with these models 4 7 4 flange portion of the Collimating Adapter into the lamp port of the microscope Using the existing hardware tighten the screw s until the flange is fully secured Note 7he installation of the Collimating Adapter will vary with microscope 1 models 4 7 5 Remove the protective cap from the output end of the Light Guide Insert the Light Guide into the input portion of the Collimating Adapter until it is flush with the adjustable insert Secure the Light Guide to the Collimating Adapter by tightening the thumbscrew Do not over tighten A Note Light Guide has a minimum bend radius of 1 6 inches 40 0mm Bending or coiling the Light Guide tighter than this radius will result in L permanent damage to the Light Guide Figure 11 Collimating Adapter LUMEN DYNAMICS 035 00465R rev 5 X Cite Fluorescence Illumination In Control 5 Powering Up Powering Down e The system will be fully operational under fluorescent or incandescent lighting e The system will be fully operational under zero lighting darkness e The system
60. in LED screen When in high speed triggering mode the sync signal is an acknowledgement of a received command When the trigger is set to External the system will accept an external signal control to the selected LEDs Refer to Table 8 LUMEN DYNAMICS 42 035 00465 5 X Cite Fluorescence Illumination In Control D When the trigger is set to GLOBAL the system will accept a trigger signal through a single pin to control all LEDs that are set to GLOBAL Refer to Table 8 A Note When selecting External or Global trigger the LEDs will not turn on until a trigger signal is present The LEDs will accumulate hours while in External or Global trigger mode To prevent unnecessary hour accumulation on LED modules while using the XLED1 system users are advised to enable these XLED1 advanced triggering modes only during the window of time that will be used for such purposes Subsequent to using the advanced triggering capabilities of the XLED1 system the user should ensure that triggering is OFF in the trigger configuration screen GM ee deed When the trigger is to Internal as indicated by zeroes the user can define the DELAY TIME ON TIME OFF TIME AND SYNC OUT TIME To change the numerical values of the times press the up and down arrows or touch the box next to the value to open up a key pad screen where you can en
61. inates when the Light Guide is properly inserted Indicates a system alarm user should navigate to the Alarm Screen to determine the source of the alarm Illuminated when any LED Module is on LUMEN DYNAMICS 2 4 035 00465 5 X Cite Fluorescence Illumination In Control 6 1 Main LED Screen Touchscreen Locked Light Guide Inserted Alarm ea LED on aE LOCK ALL LEDs TURN ON ALL LEDs 3GER Text Entry Enter Number 88 Enter LED Text Name 385nm 0 Wavelength Figure 12 LED Screen GUI LUMEN DYNAMICS 2 5 035 00465R rev 5 X Cite Fluorescence Illumination In Control 6 1 1 main screen displays a section for each LED module e g UVX BGX and RLX these can be renamed to desired fluorophores Click on the desired wavelength name to view the wavelength range or to rename the LED Enter a name or number and press OK The display will now be changed BB o0 LightGuideinserted y alarm UB LOCK ALL LEDs TURN ON ALL LEDs ee TRIGGER MAINTENANCE 6 1 2 Each LED module section has Unlocked grey Locked green OFF grey and ON green features The Unlock Lock pads unlock or lock that individual LED module section In lock mode all settings coarse and fine intensity percentage window and wavelength window for that LED module are locked and cannot be changed 6 1
62. indow Descriptions GUI under 1096 To check if an LED has failed in this case simply move the intensity slider to 100 to check if it is functional WITH CAUTION p ee TE T TE l r Note The alarm will not be triggered if an LED fails when the intensity is set to 7 Operating the Optional Touch Screen Controller Caution Only connect the LGDI Touch Screen Controller to the XLED1 via rear as per Figure 2 LUMEN DYNAMICS 3 7 035 00465R rev 5 x lt Cite Fluorescence Illumination In Control 7 1 1 Touch Screen Controller is a touch screen interface to control the XLED1 Screen Sitemap gt LogIn refer to section 7 2 gt Main LED Screen refer to section 7 3 gt Edit LED Name refer to section 7 4 gt Set LED Triggers refer to section 7 5 gt Trigger Graphs refer to section 7 6 gt Maintenance refer to section 7 7 gt Alarm Information refer to section 7 8 gt System Information refer to section 7 10 Note all screens have a top bar indicator Touch Screen Locked Indicates that all screens are locked refer to section 7 3 for detailed instructions e Light Guide Inserted Illuminates when the Light Guide is properly inserted e Alarm Indicates a system alarm user should navigate to the Alarm Screen to determine the source of the alarm refer to section 7 8 LED on Illuminated when any LED Module is on 7 2 Log In Scre
63. ing hub key hanger located on the back panel of XLED1 Figure 5 Locking Hub 4 2 3 LED Modules are shipped with their unique Drivers Both components must be installed at the same time Refer to Figure 4 for module and Dichroic locations Caution Do not touch the LED lens surface Skin oils and scratches can cause the optical output to decrease Caution The Dichroic window operational life can be significantly shortened if it is handled incorrectly Do not touch the window surface Skin oils and scratches can cause the window to fail prematurely Refer to section 12 3 for handling and cleaning instructions LUMEN DYNAMICS 1 3 035 00465R rev 5 X LCire Fluorescence Illumination In Control 4 2 4 Toinstall the LED Module and Driver first remove the protective LED lens cap from the LED Module Align the LED module driver male pins with the female socket connection and gently push down into place Tighten the Philips Head screws until a positive force is felt and apply 1 4 turn DO NOT OVER TIGHTEN THE SCREWS If using a calibrated torque meter Lumen Dynamics recommends a torque value of 5 7 Ib in t 0 3 Ib in 4 2 5 1 there is a mismatch installation of either standard powered or high powered Drivers the corresponding LED will not operate 4 2 6 Install the Dichroics by inserting through the slot and lightly press down until a positive force is felt Ensure to hand tighten the locking screw do no
64. is subject to change without notice and does not represent a commitment on the part of the authors LUMEN DYNAMICS 035 004658 5 X Cite Fluorescence Illumination In Control Table of Contents 1 peg cee 1 2 Safely ieee 2 2 1 GIOSSArY OL Symbols tbe 2 2 2 Safety Precautiofis rci etes De euet de 3 2 9 Principles of ESD Control niei tte terere e RR 5 3 Getting Started ecc 7 3d ALEDT FON Pano e O 8 3 2 KLEDT Back Panel 9 3 3 Optional Touch Screen Controller Swivel Stand 10 4 installation PROC OSS coca access ccc crews red 11 41 General Installation ierit nete deca nies A ee anaes 11 4 2 LED Module Driver and Dichroic 12 4 3 Installing Excitation Filter 16 4 4 Graphical User Interface 11 innen 18 45 Light Guide Auto Detect nnns sn nnn sen tenens 4 6 Inserting Removing Light Guide 4 7 Installing the Collimating 5 Powering Up Powering 6
65. ivers anddichroics 6 4 2 on any one of the green lit Modules to retrieve the pop up window showing the selected Modules present attributes e i Displays LED Module wavelength range in nm J FWHM Full Width Half Max e g depicts LED peak wavelength FWHM the useful bandwidth of the LED e Type Identifies the LED Module as either Standard Power or High Power version which is determined by the LED type e Current Displays the current working temperature of the LED module in C Cutoff Temp indicates the maximum specified working temperature of the LED module in C e LED Used Records the current accumulated LED module illumination hours Serial Number Displays the serial number of the LED module Mfg ON Displays the manufacturing date of the LED module The touch pads are for navigating to the various screens LUMEN DYNAMICS X Cite Fluorescence Illumination In Control XLED1 Control Panel TouchScreen Locked 7 Light Guide Inserted Alarm LED on CHANGE PIN Reflect 327nm 490nm Transmit 500nm 750nm SYSTEM DICHROIC DATA WINDOW RETURN TO LED SCREEN TRIGGER Figure 17 Maintenance Screen Dichroic Data GUI 6 4 3 When clicking a Dichroic module icon a Dichroic data window will display the following attributes Edge specified in nm top tab of the window Reflect the wavelength range that the Dichroic will reflect specified in nm Transmit the wavelength r
66. ked Light Guide Inserted Alarm RETURN TO LED SCREEN Figure 23 LED Name Edit Screen Touch Screen Controller 7 41 Edit screen displays a touch key pad to enable the user to rename the LED modules if desired e g DAPI FITC TRITC etc Once the name has been entered press RETURN TO LED SCREEN to go back to the Main LED Screen 74 2 The name is limited to 8 characters 7 4 3 To display the centre wavelength as the wavelength name press the USE WAVELENGTH AS NAME pad LUMEN DYNAMICS 4 1 035 00465R rev 5 Fluorescence Illumination In Control 7 5 Set LED Triggers Screen VIEW TRIGGER GRAPH Repeat Loop E ON TURN ALL 5 RETURN TO LED SCREEN 7 5 1 7 5 2 7 5 3 M MAINTENANCE Figure 24 LED Trigger Screen Touch Screen Controller The screen displays individual Trigger window sections for each LED module e g UVX BDX BGX and RLX Touch the top of a wavelength section to open that selected window In each window section the user can change the trigger designation to OFF Internal indicated by time units External and Global Press the CHANGE TRIGGER pad to select a trigger designation Each LED module can only have one trigger designation The triggers are described below The default trigger designation is set to OFF in order to allow control of the system from the ma
67. lution Note Avoid using any type of pressurized air nitrogen systems for cleaning as there is a possibility of leaving an oily or chemical film on the Dichroic surface Dichroic Optical Window Cleaning Procedure e Wear powder free latex gloves or finger cots to protect hands from solvents and prevent window from contamination e Blow off dirt and dust on glass surface with rubber dust blower Use a lens tissue or a lint free cotton swab Saturate the lens tissue or swab with organic solvent A CAUTION Before using any solvent consult the manufacturer s Materials Safety Data Sheets MSDS and your internal Health and Safety Advisor for proper handling and storage Note Never re install the Dichroic before the solvent has evaporated LUMEN DYNAMICS 67 035 00465R rev 5 X LCire Fluorescence Illumination In Control 12 5 Tips to Prevent Premature Degradation of Light Guides 12 5 1 Liquid Light Guides have a typical useful life of 2 3 years when installed and handled properly The formation of bubbles is one of the most common reasons for a Light Guide to degrade prematurely and result in a reduction in illumination intensity Bubbles form without warning usually due to overheating and or mechanical stress to the Light Guide Below are some simple tips to avoid overheating and stressing the Light Guide 12 5 2 The Light Guide has a minimum bend radius of 1 6 inches 40 0mm Bending or coiling the Light Guide tighter
68. m types according to the danger they pose to the operator system or experiment 1 Critical Do not continue process alarm shall not be clearable until Alarm Condition is corrected Contact http www ldgi xcite com asc php 2 Major operation may continue but depending on specific alarm condition functionality of unit may be limited or impossible Contact http www ldgi xcite com asc php 6 6 3 Alarm Information screen displays icons for the alarm codes which will light up when triggered 6 7 Clearing alarms 6 71 8 visual alarm indicators on the Alarm Code Screen refer to Figure 19 are indicators only and do not functional as alarm clearing buttons pads 6 72 The GUI system does not provide an audible alarm to alert the operator of Light Guide not inserted condition 6 7 3 following visual alarms will clear automatically when the problem subsides Temp Exceeded Min Temp Under Limit LED Module Fail Light Guide Not Present 6 8 System Information Touchscreen Locked Light Guide Inserted Alarm wa LED on Software Versions 1 4 0 1 1 0 1 1 0 ALIGN SerialNumber SCREEN SELF TEST 00129 Volume Brightness E ii RETURN TO MAINTENANCE SCREEN Figure 20 System Information GUI LUMEN DYNAMICS 3 6 035 00465 5 X LCire Fluorescence Illumination In Control 6 8 1 System Scree
69. max 4 digits and press GO Re enter the new PIN number to verify and press GO A pop up message will appear if the wrong PIN number is re entered Re enter the correct new PIN number and press GO 7 3 Main LED Screen Touchscreen Locked Light Guide Inserted eal Alarm LED LOCK ALL LEDs l TRIGGER MAINTENANCE Figure 22 Main LED Screen Touch Screen Controller LUMEN DYNAMICS 39 035 00465R rev 5 X Cite Fluorescence Illumination In Control 7 3 1 main screen displays a section for each LED module e g UVX BDX BGX and RLX these can be renamed to desired fluorophores refer to section 7 4 Tap on the desired wavelength name number to access the Edit LED screen Click on the desired wavelength name to view the wavelength range or to rename the LED Enter name or number and press OK The display will now be changed ooo tightGuideinsertead J alarm UB o LOCK ALL LEDs OK _ TURN ON coil ALL LEDs 2T _ TRIGGER MAINTENANCE 7 3 2 Each LED module section has Unlocked grey Locked green OFF grey and ON green features The Unlock Lock pads unlock or lock that individual LED module section In lock mode all settings coarse and fine intensity percentage window and wavelength window for that LED module are locked and cannot be changed 7 3 3 Ea
70. n AR MEE NE EXPE ES When the trigger is set to Internal as indicated by zeroes the user can define the DELAY TIME ON TIME OFF TIME AND SYNC OUT TIME To change the numerical values of the times click the up and down arrows or click the box next to the value to open up a popup Edit Entry window where you can enter the desired numeric value To change the time units click the CHANGE UNITS pad and select a time value unit of microseconds milliseconds or seconds Note When changing the time value units selecting a value unit will change all 4 times associated with that LED to that unit Each LED module can only have one time domain unit r F Once all the timing parameters have been defined for each LED module click the REPEAT LOOP pad to ON green for automatic looping For manual use or test purposes ensure the REPEAT LOOP pad is OFF and use the START and STOP pads to start and stop the process A Note Ensure that the trigger is set to OFF in order to control LEDs from the 1 screen trigger graph can be viewed refer to section 7 6 for each LED module by clicking on the VIEW TRIGGER GRAPH ADDITIONAL TRIGGER SET UP INSTRUCTIONS Live Cell Configuration owners should refer to section 8 0 for Live Cell Mode Instructions
71. n Shows an overview of the complete system GUI Control Panel XLED1 Errors Window gt Displays error description sent by the XLED1 detected during the power on self test POST and errors detected during operation Refer to Table 7 for error window descriptions Software Versions Window gt Displays the current software versions installed in the XLED1 Pulse Generator GUI Control Panel Serial Number Window gt Displays the serial number of the XLED1 LCD Settings Window gt Volume Brightness and Timeout sliders are not functional when using the GUI All sliders will function and change the values if connected to the XLED1 Touch Screen Controller POWER ON SELF TEST gt Disabled in GUI Control Panel ALIGN TOUCH SCREEN gt Disabled in GUI Control Panel Error Window Error Description LED wavelength over temperature LED module is above allowable working temperature LED module is off when system is told it is on only valid LED wavelength current alarm when not in trigger mode or LED module is on when system is told it is off only valid when NOT in trigger mode LED wavelength under temperature LED module is below allowable working temperature LED wavelength NVM error LED module memory is experiencing problems LED wavelength ADC error Internal error LED wavelength Type Mismatch LED module LED driver types are mismatched Table 4 Error W
72. ng the Touch Screen Controller Refer to section 6 6 for alarm information if using the GUI Control Panel LUMEN DYNAMICS 2 2 035 00465R rev 5 X Ci Fluorescen 5 1 7 5 1 10 ce Illumination In Control During the POST sequence the system stabilization will Check for number of LED Modules installed Read LED module ID check hours and check System LED NVM write capability Ensure LED and driver type match Check LED module thermistor Check for alarms Check for Pulse Generator Module Check for connected Light Guide Check for connected Touch Screen Controller If using the GUI Control Panel once the system runs through and has exited the POST sequence the system is ready to use there is no reset or warm up sequence required To power down the system after use ensure all LEDs are off click on the log out pad on the GUI Control Panel and push in the master power button to turn the XLED1 system off All previous settings in the GUI Control Panel will be saved If Using the Touch Screen Controller 5 1 11 5 1 12 5 1 13 5 1 14 5 1 15 If using the optional Touch Screen Controller once the system runs through and has exited the POST sequence the Touch Screen Controller screen will turn on automatically and the system is ready to use there is no reset or warm up sequence required If the Light Guide is not fully inserted the Light Guide indicator LED will be RED and there will be 3 bee
73. nt or powder free gloves or finger cots e Anorganic solvent such as reagent grade isopropyl alcohol or lens cleaning solution Note Avoid using any type of pressurized air nitrogen systems for cleaning as there is a possibility of leaving an oil or chemical film on the lens surface Lens Assembly Cleaning Procedure e Wear powder free latex gloves or finger cots to protect hands from solvents and prevent lens from contamination Blow off dirt and dust on lens surface with rubber dust blower Use a lens tissue or a lint free cotton swab Saturate the lens tissue or swab with organic solvent CAUTION Before using any solvent consult the manufacturer s Materials Safety Data Sheets MSDS and your internal Health and Safety Advisor for proper handling and storage Note Never re install the lens assembly before the solvent has evaporated 12 4 Handling and Cleaning of the Dichroic Cleaning of the Dichroic is not generally required However if any visible contamination or finger prints appear in the Dichroic optical window the contaminants may cause reduced light output and further damage to the system Light output degradation may also result from scratches digs and other coating damage on the Dichroic window surface Refrain from touching the optical surface with any foreign object which could scratch the optical window Cleaning Procedure for Dichroic e The Dichroic includes a coated substrate Dichroic optical window and module
74. ntistatic bag a disposable antistatic mat or a grounded metal surface If possible wear an antistatic grounding strap connected to a metal surface on the chassis when you replace any LED module or LED driver Refer to section 2 3 for understanding the principles of ESD control LUMEN DYNAMICS 4 035 00465R rev 5 X LCire Fluorescence Illumination In Control 2 3 Principles of ESD Control 2 3 1 2 3 2 2 3 3 The basics of electrostatic charge discharge types of failures ESD events and device sensitivity may be summarized as follows e Virtually all materials even conductors can be triboelectrically charged an electric charge generated by friction e level of charge is affected by material type speed of contact and separation humidity and several other factors e Electrostatic discharge can create catastrophic or latent failures in electronic components e Electrostatic discharge can occur throughout the manufacturing test shipping handling or operational repair processes e Component damage can occur as the result of a discharge from the component as well as a direct discharge to the component e Components vary significantly in their sensitivity to ESD The best method of preventing ESD is to use an ESD wrist strap a grounding mat or a metal table However since most users do not have access to such items we have provided the following guidelines to help reduce the risks and levels of ESD
75. o figures 4 22 23 for configuratio LUMEN DYNAMICS 5 7 035 00465R 5 X LCire Fluorescence Illumination In Control 5V signal applied to Port 2 trigger this will turn on LED Module 2 when LED Module 2 is in external trigger mode OV signal applied to Port 2 trigger this will turn off LED Module 2 when LED Module 2 is in external trigger mode 5V signal applied to Port 3 trigger this will turn on LED Module 3 when LED Module 3 is in external trigger mode OV signal applied to Port 3 trigger this will turn off LED Module 3 when LED Module 3 is in external trigger mode 5V signal applied to Port 4 trigger this will turn on LED Module 4 when LED Module 4 is in external trigger mode OV signal applied to Port 4 trigger this will turn off LED Module 4 when LED Module 4 is in external trigger mode 5V signal applied to Global trigger this will turn on all LED Modules which are in global trigger mode OV signal applied to Global trigger this will turn off all LED Modules which are in global trigger mode External Sync Output LED Module 1 is not necessarily the lowest wavelength installed refer to figures 4 22 23 for configuratio 2 signal will be produced Port 1 sync when LED Module 1 has been triggered OV signal will be produced Port 1 sync when LED Module 1 has not been triggered A 5V signal will be produced on Port 2 sync when LED Module 2
76. on 11 for Dichroic replacement 7 7 5 new Dichroics the reflect and transmit values will not be automatically updated refer to Section 6 5 for these values The data cannot be entered into the Touch Screen Controller manually The system will light up the new Dichroic in red This means the data is not available but the system will still be functional To update the values use the GUI on your PC refer to sections 6 4 3 to 6 4 5 LUMEN DYNAMICS X LCire Fluorescence Illumination In Control 7 8 Alarm Information Alarms Description Severity Recoverable Max Temp Exceeded disi ED Module Over Temperature Critical Yes rotection Min LED Module Under Temperature Min Temp Exceeded Protection Major Yes LED Module Fail LED Module Failure Critical No Light Guide Not Light Guide Removed or not fully Major Vas Present inserted Comms Error Internal Communication Error Critical No Watchdog Timeout Watchdog Tripped Critical No NVM Fail Non Volatile Memory Fail Critical No System Performance System Performance Major Yes Table 5 Alarm Codes Touch Screen Controller NB o B ooo B alarm UB o Max Temp Exceeded Min Temp Exceeded LED Module Fail Light Guide Not Present Comms Error Watchdog Timeout NVM Fail System Performance re RETURN TO MAINTENANCE SCREEN Figure 28 Alarm Code Screen Touch Screen Controller LUMEN DYNAMICS 48 035 00465R rev 5 X LCire
77. onnected 5 n c Not Connected 6 Port 1 sync Ground Port 1 red BNC cable 7 Port 2 sync Ground Port 2 green BNC cable 8 Port 3 sync Ground Port 3 blue BNC cable 9 n c Not Connected 10 n c Not Connected 11 Port 4 sync Ground Port 4 white BNC cable 12 n c Not Connected LUMEN DYNAMICS 56 035 00465R rev 5 X LCire Fluorescence Illumination In Control PIN Signal name Description Location 13 Port 4 sync Port 4 Sync out LED on Low LED Port4 white BNC cable 14 n c Not Connected 15 n c Not Connected 2 Trigger i 1 1 Li 1 1 1 LI 1 1 1 gt tl Sync t1 pre sync time t2 sync delay time t3 post sync time 9 2 Trigger Input Sync Output Signals Requirements e Trigger Input Signal Requirements Low level input 0 0 8VDC o High level input 4 5 5 5VDC o Maximum input current 6 5mA e Sync Output Signal Requirements o Low level output 0 0 8VDC o High level output 4 5 45 5 VDC o Maximum output current 1000 9 3 Signal Descriptions External Trigger Input e 5V signal applied to Port 1 trigger this will turn on LED Module 1 when LED Module 1 is in external trigger mode e OV signal applied to Port 1 trigger this will turn off LED Module 1 when LED Module 1 is in external trigger mode LED Module 1 is not necessarily the lowest wavelength installed refer t
78. ontroller 020 00792 Locking Hub Key comes with Locking Hub Key Hanger CD includes User Guides Quick Start Guides 031 00036R GUI Control Panel Driver USB Drivers 019 00175R X Cite XLED1 Excitation Filter Holders set of 4 805 00069R 1mm x 1000 mm X Cite XLED1 quartz fibre SMA 805 00072R 1mm x 2000 mm X Cite XLED1 quartz fibre FC 018 00491R X Cite XLED1 trigger cable DB15 to BNCX5 Table 12 Component Part Numbers roduct LUMEN DYNAMICS 90 035 00465 5 X Cite Fluorescence Illumination In Control 20 Contact Information Lumen Dynamics 2260 Argentia Road Mississauga Ontario L5N 6H7 CANADA Tel 1 905 821 2600 Toll 1 800 668 8752 USA and Canada Fax 1 905 821 2055 xcite LDGI com www ldgi xcite com Technical Assistance Techsupport 2ldgi com For a complete listing of Authorized X Cite Distributors and Service Centers please go to the main web site www ldgi xcite com X Cite Web Store http www ldgi xcitestore com LUMEN DYNAMICS 3 1 035 00465 5
79. ponents Warranty LUMEN DYNAMICS 79 035 00465R rev 5 X LCire Fluorescence Illumination In Control 19 Component Number Table For additional LED Wavelength Modules contact your local sales representative or visit http www ldgi xcite com contact ph For the on line LED module Dichroic configurator visit tp www dgi xcite com products led Modules ph h Please ensure that you register your XLED1 at tp www ldgi xcite com registerm h t t t Description X Cite XLED1 Live Cell configuration includes X Cite XLED1 unit BLX BDX BGX GYX LED modules amp appropriate dichroics Excitation Filter Holders set of 4 3mm x 1500mm Liquid Light Guide Microscope Adaptor Live Cell Mode refer to section 8 0 X Cite XLED1 Fixed Cell 1 configuration includes X Cite XLED1 unit 010 00323R UVA BDX GYX RLX LED modules amp appropriate dichroics Excitation Filter Holders set of 4 3mm x 1500mm Liquid Light Guide Microscope Adaptor X Cite XLED1 Fixed Cell 2 configuration includes X Cite XLED1 unit 010 00324R UVX BDX GYX RLX LED modules amp appropriate dichroics Excitation Filter Holders set of 4 3mm x 1500mm Liquid Light Guide Microscope Adaptor X Cite XLED1 illumination unit only includes 010 00288R GUI Control Panel 3mm x 1500mm Liquid Light Guide Microscope Adaptor 010 00278R X Cite XLED1 Optional Touch Screen C
80. ps from the Touch Screen Controller and the error icon will illuminate To enter the system at the Log In Screen follow the instructions in section 7 2 If logged in as a Guest data will not be saved after completion of session Before powering down the system navigate to the Main LED Screen section 7 3 and press the LOG OUT pad This will ensure all data entered from the latest session is saved Failure to logout before powering down the system will result in loss of all recently changed settings To power down the system after use ensure LED button is OFF push the master power switch to the OFF position Note Ensure that users log out of the Touch Screen Controller before powering down or all entered data from the latest session will not be saved refer to section 7 3 The system is defaulted NOT to save data when logged in as Guest regardless of logging out or not LUMEN DYNAMICS 2 3 035 00465R rev 5 x lt Cite Fluorescence Illumination In Control 6 Operating the GUI Control Panel Main LED Screen Set LED Triggers Trigger Graphs Maintenance Alarm Information VV VV ON V System Information refer to section 6 1 refer to section 6 2 refer to section 6 3 refer to section 6 4 refer to section 6 6 refer to section 6 8 Note all screens have a top bar indicator Touch Screen Locked Light Guide Inserted Alarm LED on Disabled Illum
81. r materials nearby to discharge to ground rather than to critical components LUMEN DYNAMICS 5 035 00465 5 X Cite Fluorescence Illumination In Control 2 3 4 Prevent discharges that do occur from reaching susceptible parts and assemblies Package and transport susceptible devices in proper packaging and materials handling products These materials may effectively shield the product from charge as well as reduce the generation of charge caused by any movement of product within the container Such products include ESD shielded bags conductive totes and antistatic packaging materials Helpful ESD Links http en wikipedia org wiki Electrostatic discharge http en wikipedia org wiki Electrostatic sensitive device http www ipc org ContentPage aspx pageid IPC and ESDA Collaborate on Required ESD Training for Electronics Assembly Operators http www ce mag com 99ARG ESD 20Assoc175 html LUMEN DYNAMICS 6 035 00465R rev 5 X LCire Fluorescence Illumination In Control 3 Getting Started System Components Carefully unpack the system and accessories As you remove each item from the box place a check mark next to each item to ensure that all ordered components are present Store the packaging and packing materials for future use Box Contents Check List XLED1 ordered modules and dichroics USB Gabl
82. signments OUTPUT 5 5V 8mA max 56 Table 10 China ROHS I TM 77 Table 11 System Components Warranty 79 Table 12 Component Part nnaman 80 e LUMEN DYNAMICS 1V 035 00465R rev 5 X Cite Fluorescence Illumination In Control 1 Introduction The X Cite XLED1 System is intended for use in fluorescence microscopy illumination It allows researchers the ability to excite fluorescence in samples being studied and characterize their location or behaviour The X Cite XLED1 System resets the standard in LED fluorescence microscopy illumination systems The heart of the X Cite XLED1 System are the LED Modules Dichroics available for use in multiple configurations and combinations each with 20 000 hour guaranteed lifetime The systems ease of use is made possible by the innovative solid state LED technology incorporated into the design of the system along with simple to advanced triggering controls plug and play Modules and system intelligence An easy to use Graphical User Interface GUI module allows full control of each LED Also available is the optional Touch Screen Controller providing the full control and monitoring of each LED module as never before seen in such a form factor Refer to the website for all LED module driver and Dichroic locations and combinations http www ldg
83. t use mechanical tooling such as screwdrivers pliers etc Refer to Figure 4 for Module and Dichroic locations 427 M the Dichroic is inserted incorrectly the Dichroic s orientation key located on the frame will not pass through the cut out on the top plate resulting in an unseated Dichroic Refer to Figure 6 4 2 8 When changing a Dichroic the Edge value must be manually entered into the system If using the GUI Control Panel Refer to section 6 4 and Figure 18 For instructions on changing the Edge reflect and transmit values 4 2 9 f using the Touch Screen Controller to change the Edge value touch on the Dichroic to see the pop up window touch the top tab of the window and an edit screen will appear Type in the new Edge value and press GO to return to the maintenance screen The edge value is printed on the Dichroic module For new Dichroics the reflect and transmit values will not be automatically updated refer to section 6 5 for these values 4 2 10 Replace the XLED1 housing ensuring a snug fit Insert the locking hubs and using locking hub key supplied rotate the green locking hubs to the locked position Return the locking hub key to the locking hub key hanger located at the rear of the system The system will not allow the main power switch to function if the housing is not replaced properly e LUMEN DYNAMICS 14 035 00465R rev 5 X Cite Fluorescence Illumination In Control LOCKING SCREW DICHR
84. ter the desired numeric value To change the time units press the CHANGE UNITS pad and select a time value unit of microseconds milliseconds or seconds Note When changing the time value units selecting a value unit will change all 4 times associated with that LED to that unit Each LED module can only have one time domain unit 5 i g p a ae a Sh 2 4 F Once all the timing parameters have been defined for each LED module press the REPEAT LOOP pad to ON green for automatic looping For manual use or test purposes ensure the REPEAT LOOP pad is OFF and use the START and STOP pads to start and stop the process A Note Ensure that the trigger is set to OFF in order to control LEDs from the 1 screen trigger graph can be viewed refer to section 7 6 for each LED module by touching the VIEW TRIGGER GRAPH ADDITIONAL TRIGGER SET UP INSTRUCTIONS Live Cell Configuration owners should refer to section 8 0 for Live Cell Mode Instructions a In internal triggering mode the LEDs in the same time domain a time domain is either in seconds milliseconds or microseconds will be automatically synchronized The LED period determined by adding the Delay Time On Time and Off Time will be adjusted to the longest period selected In the example shown in graph LED A below the total period is 6 seconds Then as shown in graph LED B below the period
85. ugh to the microscope so that there is some slack in the Light Guide and no sharp bends e LUMEN DYNAMICS 63 035 00465R rev 5 Fluorescence Illumination In Control 12 5 6 Never leave an end cap on the output end of the Light Guide when the other end is connected to XLED1 if the unit is turned on in this condition the cap will overheat melt and or permanently discolour the quartz end of the Light Guide 12 5 7 While XLED1 is on but not in actual use i e during sample preparation or between time lapse time points it is recommended to disable the LED source by depressing turning off the LED ON OFF button refer to Figure 1 or through the GUI Control Panel refer to section 6 1 or through the Touch Screen Controller refer to section 7 3 this reduces unnecessary photon load on the liquid Light Guide Using only the shutter stop in the microscope itself protects the specimen but energy is still passing through the Light Guide What does a bubble look like 12 5 8 Depending on the size and location in the Light Guide a bubble may or may not be obvious To check for bubbles 12 5 9 Disconnect the LLG from the XLED1 and microscope adapter 12 5 10 Hold one end towards a bright window or overhead room light DO NOT use the XLED1 or any other focused light source for this test 12 5 11 Look at the quartz at the other end of the LLG a Bubble free quartz end will appear as a bright solid circle you
86. uipment Class Installation Category II Pollution Degree 2 IEC 62471 2006 amp IEC TR 62471 2 2009 Photobiological safety of lamps and lamp systems Guidance on manufacturing requirements relating to non laser optical radiation safety Electromagnetic Compatibility Immunity EN 61326 1 2006 Electrical equipment for measurement control and laboratory use EMC requirements Emissions CISPR 11 2009 A1 2010 EN 55011 2009 A1 2010 Class A Group 1 ISM Equipment LUMEN DYNAMICS 74 035 00465R rev 5 X Cite Fluorescence Illumination In Control WARNING This is a Class A product In a domestic environment this product may cause radio interference in which case the user may be required to take adequate FCC PART 15 Subpart Class Unintentional Radiators FCC Class A Digital Device or Peripheral Information to User NOTE This equipment has been tested and found to comply with the limits for a Class A digital device pursuant to part 15 of the FCC Rules These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in commercial environment This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instruction manual may cause harmful interference to radio communications However there is no guarantee that interference will not occur if installe
87. ural resources we encourage you to use the appropriate take back systems Those systems will reuse or recycle most of the materials of your end life equipment in a sound way e The crossed out wheeled bin symbol indicated above invites you to use those systems e f you need more information on the collection reuse and recycling systems please contact your local or regional waste administration e LUMEN DYNAMICS 76 035 00465R rev 5 X LCire Fluorescence Illumination In Control China RoHS 16 1 1 The following table contains substance information for the XLED1 System as required by China RoHS regulations Names and Content of Toxic and Hazardous Substances or Elements Toxic and Hazardous Substances or Elements Pb Cd Cr PBDE 21 co o Dichroics oo ER o 107 Supply Parts Name Indicates that this toxic or hazardous substance contained in all of the homogeneous materials for this part is below the limit requirement in SJ T11363 2006 x Indicates that this toxic or hazardous substance contained in at least one of the homogeneous materials used for this part is above the limit requirement in SJ T11363 2006 Enterprises may further provide in this box technical explanation for marking x based on their actual conditions Table 10 China RoHS e LUMEN DYNAMICS 77 035 00465 5 X LCire Fluorescence Illumination In Control 17 Warranty
88. ve backing and affix to the recommended location shown on the rear panel in Figure 2 4 1 2 Do not use the removable cover as a means to lift the XLED1 It is recommended that the XLED1 and the optional Touch Screen Controller are mounted on flat stable surface platform adjacent to microscope 4 1 8 Mf purchased connect the optional Touch Screen Controller to the display module connection provided at the rear of the XLED1 panel Refer to Figure 2 Note Do not use both the GUI Control Panel and the Touch Screen Controller 1 at the same time 4 1 4 If Triggering is required connect the 15 HD DB15 trigger I O breakout cables to the trigger input and sync output connections located on the back XLED1 panel refer to Figure 2 4 1 5 If interfacing with the GUI Control Panel connect the computer to XLED1 via the USB connection located on the back panel 4 1 6 Connect the AC cable to the AC connector on the back of the XLED1 panel e LUMEN DYNAMICS 1 1 035 00465R rev 5 X Cite Fluorescence Illumination In Control 4 2 LED Module Driver and Dichroic Installation The XLED1 system ships with the LED Modules Drivers and Dichroics uninstalled and in separate boxes These need to be installed according to the instructions below Online configurator at http www ldgi xcite com products xled1 configurator php for module configurations and locations Location refers to the positions identified in Figure 4 showing an

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X-Cite® XLED1 User Guide