HCI-40 Integratable User Manual
Contents
1. 7 3 7 Installing the COs Cylinder ttr ettet es ne rrr pe ned 7 3 8 Removing Cylinder ei e eti ete eei ade A eevee 7 3 9 Cleaning the Extraction Fluid Reservoir 7 3 10 Calibration of the Optical Sensors 8 CHAPTER 4 Concentrator Operation nn nennen sess ssa nasi 9 4 1 LCD Control Panel a a erit rent iet erento cesta aa baee ea 9 4 2 Setting the Extraction Fluid nnne nennen nennen nennen nini 9 4 3 Concentrating a Liquid Sample 10 4 4 Additional Sample 5 S u aS u nna nnne nennen nennen 10 4 5 Concentration Cell Decontamination a 10 4 6 Concentration Cell Storage ie ente i ia ni ar ease 11 A T 5 11 CHAPTER S Troubleshooting irrito terere rh eh t 16 Revision 05 23 11 Www innovaprep com i CHAPTER 1 Product Overview This2. 30 Formaldehyde 25 Formic acid Ax 2 Ax E x 810 e amp zs 2 5 EM OU zo gt m 2 aA o A a 22 JHE 2 gt 5 E 8 ples se 2 R Recommended 2 5 n e 2 O S gt Z n L Limited Exposure oe NR Not Recommended ER 5 gt E ES U Unknown Wos eel 2 25 Z Ethyl Alcohol W L R R R R R R 15 Ethyl alcohol U R R R R 95 Ethyl alcohol U L R R R Ethylene dichloride NR NR R R L Ethylene glycol NR L R R L Ethylene oxide NR NR L R L 2 Formaldehyde L L L L T 5 5 g 2 A R R R R R R U R R R U R R R R R NR R 50 Formic Acid NR L R Freon NR R R NR R R R Gasoline NR R R R R R R Glycerine NR R R R R R R Glycerol U R R R R R R Hexane NR R R L R R R Hexanol U 5 Hydrochloric acid NR R R R R 25 Hydrochloric acid NR R R 37 Hydrochloric acid NR NR NR NR NR R R 25 Hydrofluoric acid NR R NR R 30 Hydrogen peroxide R R R L R R Iodine solutions U R R NR U R Isobutyl alcohol U R R R R U R Isopropanol U R R R L R R Isopropyl acetate NR R R Isopropyl alcohol NR L R 2 RR NR L R R Isopropyl ether L Jet Fuel 640A NR R R R R L R U R Kerosene NR R R R R R R L R Lactic acid R R R R R L L R R Methyl acetate NR NR R R R R R
3. md The Macro to Micro Interface INNOVAPREP InnovaPrep Hydrosol Concentrator Integrated Platform Operator s Manual Table of Contents CHAPTER Product OVerViOW 1 1 T Prod ct Deseriptiol re rete c tte ete ep pent 1 1 2 Applications entere natis eq inte 1 1 3 How the InnovaPrep Hydrosol Concentrator Operates 1 1 4 InnovaPrep HISLtOFLy ee reete re Moock ee aa Rester te e nh TO eite 1 CHAPTER2 Description of the InnovaPrep Concentrator nnne nnne 1 2 1 Front Pariel oa a RR TERR E n REESE h 1 22 Internal Compotients a toti eiat ea veste aae Ee akan Sasa 3 CHAPTERS Installation is treten m mtr EE aqa aa 5 3 1 Materials Provided 5 3 2 Materials Required But Not Provided 5 3 3 Installing a Concentration Cell 5 3 4 Uninstalling a Concentration Cell 6 3 5 Filling the Extraction Fluid Reservoir 6 3 6 Draining the Extraction Fluid Reservoir
4. A hold a waste container under the tube Start Allow the fluid to purge until the foam is of the proper consistency Stop 3 6 Draining the Extraction Fluid Reservoir 1 2 3 4 5 Attach a length of tubing to the Drain port and place the other end in an appropriate waste container If there is no pressure on the extraction fluid raise it to approximately 50 PSI Switch the valve to Off Slowly switch the Extraction fluid valve to Drain Control the flow rate using the Extraction fluid valve When the extraction fluid has finished draining switch the Extraction fluid valve to Off 3 7 Installing the CO Cylinder Tolo pub 6 Set the Extraction fluid valve to Run Set the CO valve to Off Place the CO cylinder into the bottom CO cylinder housing Screw in the bottom housing until resistance is met then rapidly screw the housing in until snug If the regulator is set to the correct pressure then proceed to step 6 If not use the 7 mm nut driver and slowly turn the Extraction fluid pressure regulator adjustment screw clockwise until the desired pressure is reached When you are ready to pressurize the extraction fluid switch the CO valve to Run 3 8 Removing the Cylinder l 2 3 If there is any pressure shown on the Supply Pressure gauge slowly turn the valve to CO Vent to vent any remaining CO out of the cylinder If no gas flows the Extraction fluid pressur
5. C at 10 psi for 10 minutes Ramp up the autoclave by increasing pressure approximately 1 5 psi min for 30 minutes to 123 C Hold the autoclave at 123 C for 50 minutes Do not exceed 124 C Slowly ramp down the temperature of the autoclave by exhaust pressure release of approximately 0 75 to 1 0 psi min to 104 C Hold the autoclave at 104 C for at least 20 minutes Bring the module to room temperature Tighten all sanitary connections Luer fittings or any other clamps or connections if they are fitted in the module 4 6 Concentration Cell Storage Concentration cells are generally stored in 0 01 N sodium hydroxide rinsed with sterile filtered water prior to use Please contact InnovaPrep 816 619 3375 for assistance in selection of other storage fluids or with dry storage 4 7 Cell Selection Table 4 1 contains a chemical resistance chart for the various components of the HCI 40 system This chemical resistance chart is intended for use as a guide not as a guarantee of chemical compatibility Variations in temperature concentrations durations of exposure and other factors may affect the use of the product It is recommended that testing be performed under the user s specific conditions Revision 12 27 13 www innovaprep com 12 The cells should be Table 4 1 Chemical Resistance Chart g W s 2 3 maj 8 al 2 gt g
6. L R Methyl alcohol IE L R R R L R U R 98 Methyl alcohol L L R R R RJ L RQj U R Methyl cellosolve NR L L LR LR REX U R Methyl chloride NR NR R NR R L L R U R Revision 12 27 13 www innovaprep com gt lt 5 emo sic 2 2 2 Jags 23 s 22 g g s R Recommended s 9 sal gt c o L Limited Exposure 2 5 lt 2 5 NR Not Recommended a 9 ES 9 amp 5 2 U Unknown os z 1221 Z Methyl ethyl ketone R N R R UJR Methyl formate U NR L R R U U U U Methylene chloride NR L R R R L L INR R N methyl 2 pyrrolidone U NR R R R U L U U Mineral spirits NR R R R R R R U R Monochlorobenzene U L R L R U U U U 5 Nitric acid L L R R 25 Nitric acid NR L IR 6 N Nitric acid NR NR NR R R 70 Nitric acid NR NR NR R INRI R Conc Nitric acid NR NR R INR R Nitrobenzene NR L L NR R Nitropropane U U U U U Oils mineral R R R U R Pentane NR R L R R 25 Perchloric acid NR U R Perchloroethylene NR U R Petroleum based oils NR R R R Petroleum ether NR R U 0 596 Phenol NR NR L L R 1096 Phenol NR NR L NR R 25 Phosphoric acid NR L NR U R 1 N Potassium hydroxide U L L R R 2596 Potassium hydroxide U L L R R 50 Potassium hydroxide U L R Propanol U R R Pyridine NR NR R Silic
7. Out Port A 1 4 28 flat bottom port labeled Permeate Out is located two ports to the left of the Feed In port on the right hand side approximately half way up from the bottom internal plate A section of tubing with a 1 4 28 flat bottom connector should be attached to this port and placed into an outside reservoir to catch the permeate fluid Revision 12 27 13 www innovaprep com 4 Figure 2 2 InnovaPrep Hydrosol Concentrator Integrated Platform Internal Components Back View Figure 2 3 InnovaPrep Hydrosol Concentrator Integrated Platform Internal Components Top View Revision 12 27 13 WWw innovaprep com 3 CHAPTER 3 Installation Included in this chapter are the general instructions for the setup of the InnovaPrep Hydrosol Concentrator Integrated Platform Inspect each component when unpacking If damage is observed do not use the instrument and contact your shipping representative immediately Remove any protective packaging that may be present around the instrument Examine the packaging material to be sure that materials that have been provided see the list below are removed 3 1 Materials Provided Component Qty InnovaPrep concentrator Programming Cable 7 mm Nut Driver Pre filter Kit Tubing supply kit 12 g food grade C0 Cartridge N 3 2 Materials Required But Not Provided The reagent for sample extraction is required in order to use the system The user must provide this A sta
8. adjusts the extraction fluid flow rate and is identified as Ext fluid flowrate The right needle valve adjusts the CO flush flowrate 2 1 7 Hollow Fiber Concentration Cell The concentration cell is positioned vertically just to the left of the carbon dioxide cylinder on the right hand side of the front panel The concentration cell is connected to the system at ports A B and C and to the retentate valve and a fluid sensor located directly above the retentate valve 2 1 8 Retentate Valve The bottom retentate tube on the hollow fiber concentration cell runs through the retentate valve that is located directly below the concentration cell This valve controls concentrate fluid flow from the concentration cell Revision 12 27 13 Www innovaprep com 3 2 1 9 Concentrate Reservoir if present Directly below the retentate valve is the concentrate reservoir if present This reservoir can be removed and the concentrate can be captured into a small sample tube or the reservoir can be connected with an outlet tube for integration into an automated system 2 1 10 Liquid CO Cylinder A 12 gram liquid CO cylinder is required for carbonating the extraction fluid The cylinder is installed into the black cylinder housing located on the far right side of the front panel 2 1 11 Ports A B and C Port A is the feed port the sample is pumped into the concentration cell through this port Extraction fluid also enters the cell through this port Por
9. as shown 3 Attach the top port of the cell to port A on the instrument 4 Attach the upper permeate port on the concentration cell to port B on the instrument 5 Attach the lower permeate port on the concentration cell to port C on the instrument 3 4 Uninstalling a Concentration Cell To uninstall a concentration cell refer to Figure 3 1 and use the following procedure 1 Detach connectors A and B and place caps plugs into the ports 2 Detach connector C and place caps or plugs into the ports 3 Detach the tubing from the bottom port of the concentration cell and replace with a cap or plug Note The concentration cell must be filled with an appropriate storage fluid and capped prior to storing InnovaPrep recommends a 0 01 sodium hydroxide solution Contact InnovaPrep regarding questions about specific membrane types 816 619 3375 3 5 Filling the Extraction Fluid Reservoir l cem 10 If the extraction fluid pressure will remain the same it is not necessary to adjust the Extraction fluid pressure regulator prior to filling however if a lower pressure is desired begin by inserting a 7 mm nut driver into the opening for the extraction fluid pressure regulator and turning two or more counterclockwise turns to reduce the pressure to less than the desired pressure if resistance is met stop turning Slowly set the CO valve to Reservoir vent If there is still fluid in the reservoir be prepared for foam to flow o
10. assistance and troubleshooting InnovaPrep LLC 132 E Main St Drexel MO 64742 Phone 816 619 3375 info innovaprep com www innovaprep com Revision 12 27 13 www innovaprep com 17
11. chapter contains a product description of the InnovaPrep hydrosol concentrator and a brief description of instrument operation 1 1 Product Description The hydrosol concentrator shown in Figure 1 1 quickly and efficiently concentrates waterborne bacteria viruses DNA toxins protozoa or other particles of interest from large liquid sample volumes into liquid volumes as small as 100 microliters smaller volumes are achievable in some applications The system uses a novel elution method to recover organisms that have been captured onto hollow fiber membrane filters into very small volumes Figure 1 1 Hydrosol Concentrator Integrated Platform 1 2 Applications The InnovaPrep technology has application anywhere that analysis of low concentrations of particles is needed The primary area of application is for preparing and concentrating bacteria viruses DNA and other particles of biological origin including pathogens for subsequent analysis by microbiological methods or with microbiological detection devices 1 3 How the InnovaPrep Hydrosol Concentrator Operates The InnovaPrep system uses a novel wet foam elution method to recover organisms that have been captured onto hollow fiber membrane filters into user settable volumes as small as 100 micro liters smaller volumes are achievable in some applications 1 4 InnovaPrep History The technology was developed by a team of veteran aerosol research scientist
12. O 25 amp gj a gt E 5 22 2 56 2 R Recommended 5 u d 2 BU o T fs av gt 2 a L Limited Exposure So u NR Not Recommended 8 RE 8 E gt U Unknown SS 5 2 5 acid L L R R R R NR L 2596 Acetic acid NR NR R R R R NR L Acetic acid glacial R R RR LIL Acetone NR Acetonitrile NR NR R L Dilute Ammonium hydroxide R R R R R R Conc Ammonium hydroxide L R R R R R Amyl acetate R INRI R R E R Amyl alcohol R L R R R R Aniline R INR R R R R Benzene R L R R R L Benzyl alcohol NR R L Boric acid R R Brine RI IR R R R R R Bromoform U Butyl acetate Butyl alcohol Butyl cellosolve Butylaldehyde Carbon tetrachloride Z Aegre wg Cellosolve EM NR L R RR U U Chloroacetic acid NR R Chloroform NR L R Chromic acid NR L Cresol NR R Cyclohexane R R Cyclohexanone R R R L Diacetone alcohol Dimethyl formamide Dimethylsulfoxide 1 4 Dioxane R Ethers NR L TL RI R Ethyl acetate NR R R R L zal aleae alax alas x 2 2 7 4 22225 tester 2 G 7 Pluorocarbon F Revision 12 27 13 WwWwW innovaprep com
13. PTER 4 Concentrator Operation Included in this chapter are the general instructions for operation of the InnovaPrep Hydrosol Concentrator Integrated Platform 4 1 LCD Control Panel Control of the InnovaPrep CONCENTRATOR is performed using the LCD control panel as shown in Figure 4 1 Figure 4 1 InnovaPrep CONCENTRATOR LCD Control Panel 4 2 Setting the Extraction Fluid Volume 1 Set the extraction fluid pressure to desired level using a 7 mm nut driver inserted in the port to the left of the Extraction Fluid Pressure gauge turn slowly until desired pressure is reached 2 Wait a minimum of 20 minutes for the to dissolve into the extraction fluid 3 Perform a Foam Line Purge by following the menu path below Run Settings Extract settings Foam line purge The LED panel will instruct you to Disconnect cell at port A hold a waste container under the tube Start Allow the fluid to purge until the foam is the proper consistency Stop 4 Set Foam timer value in seconds in the Extract settings menu Revision 12 27 13 WWW innovaprep com 10 e Settings e Extract settings Foam timer set to 0 1005 5 Disconnect the cell at port A and hold a tare weighed vile under the tube coming out of port A Perform a test extract from the extraction settings menu 7 Weigh the vial the volume of extraction fluid should be approximately 100 uL adjust the foam flow rate valve up or down as necessar
14. cated in the lower right hand corner of the front panel Fill is used to fill the extraction fluid reservoir Drain is used to drain the extraction fluid reservoir and Vent is used both to vent gas from the extraction fluid reservoir and to vent gas from the CO cylinder 2 1 4 Control Valves Two red faced control valves are located below the peristaltic pumps These control the flow of the extraction fluid and carbon dioxide The desired position should be selected by slowly rotating the valve Built in positioning detents allow the valves to snap into place ensuring correct positioning of the valves The position settings for each of the control valves described below The Extraction fluid valve has four positions as follows Revision 12 27 13 www innovaprep com 2 Fill In this position the extraction fluid reservoir is connected to the port labeled Fill The reservoir can be filled through this port Note Vent reservoir pressure completely prior to switching valve to this position Run In this position the extraction fluid reservoir is connected to the foam valve and is ready for extraction Drain In this position the fluid can be drained from the extraction fluid reservoir to the port labeled Drain Off In this position the extraction fluid reservoir is disconnected from the fill drain and run fluidics The CO valve has four positions as follows vent In this position the supply lines are connected to
15. e regulator may need to be turned up When gas has stopped flowing set the CO valve to Off Remove the CO cylinder 3 9 Cleaning the Extraction Fluid Reservoir The Extraction Fluid Reservoir may require sterilization after the HSC 40 has an extended period of non use 1 2 3 Remove the concentration cell from unit Drain Reservoir Follow Section 3 5 Draining the Extraction Fluid Reservoir to remove any remaining extraction fluid Clean with 0 1 N Sodium Hydroxide a Slowly set the CO valve to Reservoir vent b Wait for the venting gas to stop and then set the Extraction fluid valve to Fill c Load a syringe with 0 1 sodium hydroxide and using a section of tubing attach it to the Fill port on the lower right hand side on the front face of the unit d Attach a section of tubing to the Vent port on the lower right hand side of the front face of the unit and place the opposite end into a waste container e the extraction fluid reservoir with approximately 75 mL of extraction fluid until fluid begins to flow out of the Vent port Revision 12 27 13 www innovaprep com 8 2 Let the 0 1 sodium hydroxide remain in the extraction fluid reservoir for 20 minutes Follow Section 3 5 Draining the Extraction Fluid Reservoir to remove the sodium hydroxide 4 Rinse the unit twice with sterile filtered laboratory water a b c Slowly set the valve to Reservoir vent Wait for the ven
16. ndard 60 mL syringe equipped with a 0 2 um filter is required for the transfer of fluids into the extraction fluid reservoir and for flushing the sample path One Syringe filter kit is provided Additional syringe filter kits Part HC05100 are available for purchase on the InnovaPrep website Instructions for loading the extraction fluid reservoir are included in Section 3 5 A 12 gram carbon dioxide cylinder is required for charging the extraction fluid reservoir two are provided These cartridges are available from the InnovaPrep website in 5 packs Part 5 51 5 in 10 packs Part HC05051 10 Instructions for installing the CO cylinder are included in Section 3 7 A personal computer with a serial port is required for uploading new protocols to the hydrosol concentrator Please contact InnovaPrep 816 619 3375 for information regarding this feature A 12 volt DC power supply is required to power the concentrator 3 3 Installing a Concentration Cell To install a concentration cell refer to Figure 3 1 and use the following procedure 1 Hold the concentration cell with the arrow on the label pointing up and the permeate ports facing toward the instrument Revision 12 27 13 www innovaprep com Figure 3 1 Concentration Cell Installation 6 2 Guide the bottom of the cell into retentate port and twist clockwise to seal the permeate ports should end up facing toward the right and away from the instrument
17. one oil R R R U R 0 1 N Sodium hydroxide R R L R R 5 Sodium hydroxide R R L L R 25 Sodium hydroxide R R L R 50 Sodium hydroxide U R L R Conc Sodium Hydroxide U NR R Sodium hypochlorite NR NR U R 5 Sulfuric acid U L NR NR R Revision 12 27 13 Www innovaprep com 5 c ez en 2 ez en ez e en e 4 msd 22 25 24 ox 2 2 SS eas soues 24 I ez es 5 6 2 worn 24 EE ox epuongrp 24 8 24 24 dd 24 24 24 Sdd 04 x wile lel ole Sd x z _ c ez en e 29 19353 ev e ez ez ez ev w 1 qO Z Z Z Z Z Z Z Z Z zZ ordures OHS BF KIS Z 04 Z 5 Z 5 8 2 2 8 ow o SEE 3 2583 3 gels egg go 5 SS 4 3 22 215 amp S 2 21218 5 58 44782 P SB s s 3 3 S 512 s gt gt 4 elol S S El E Kz El Sl e 2 x 16 www innovaprep com Revision 12 27 13 CHAPTER 5 Troubleshooting Please contact InnovaPrep for technical
18. s in response to the recognized need for a macro to micro interface between the large liquid sample volumes collected by many bioaerosol samplers and advanced detectors which require ultra low volumes InnovaPrep was founded in 2008 to commercialize this new concentration and sample preparation technology developed by AlburtyLab Inc and Page Applied Research LLC Revision 12 27 13 www innovaprep com 1 CHAPTER 2 Description of the InnovaPrep Concentrator The following chapter describes the components of the InnovaPrep Hydrosol Concentrator Integrated Platform 2 1 Front Panel The following section describes the main components of the front panel of the InnovaPrep hydrosol concentrator as is shown in Figure 2 1 Revision 12 27 13 www innovaprep com 1 Figure 2 1 Hydrosol Concentrator Integrated Platform Front Panel 2 1 1 LCD Control Panel Functions of the hydrosol concentrator are managed through a user interface LCD control panel located in the upper left hand corner of the front panel Detailed instructions on the operation of the LCD control panel are provided in Section 4 1 2 2 Pumps Two liquid peristaltic pumps are located directly below the LCD control panel The left serves as the feed pump and the right serves as the permeate pump Each pump is controlled to maintain feed and permeate pressures set through the LCD control panel 2 1 3 Extraction Fluid Reservoir Ports Three ports are lo
19. t B is the permeate purge port and port C is the permeate port The hollow fiber concentration cell should be positioned with the up arrow if present on the concentration cell label in the up position and the corresponding ports connected 2 2 Internal Components The following sections describe the serviceable internal components of the InnovaPrep hydrosol concentrator as is shown in Figures 2 2 and 2 3 A certified InnovaPrep technician should service internal components not described here Please contact InnovaPrep 816 619 3375 for assistance 2 2 1 Power Power to the hydrosol concentrator is provided through a separate external 12 volt DC power supply The power supply is connected to the 12VDC and 12VDC connectors located on the back right hand corner of the bottom plate 2 2 2 Communications Ports Communications ports are located on the same white connector block as the 12 volt input The IN ports are operated by 12v to 24v positive logic the OUT ports signal 12v high and Ov low As of software version 1 1 M the ports operate as follows N Start Run N2 Extract IN3 Not used and OUTI System busy 2 2 3 Feed In Port A 1 4 28 flat bottom port labeled Feed In is located on the right hand side approximately half way up from the bottom internal plate A section of tubing with a 1 4 28 flat bottom connector should be attached to this port from an outside reservoir containing the feed sample 2 2 4 Permeate
20. the port labeled Vent Note Switching to CO vent with a cylinder fully engaged will vent the entire cylinder Follow the instructions for removing the cylinder Run In this position the CO supply lines are connected and ready for operation NOTE it is only in this position that the Extraction fluid pressure gauge indicates the pressure on the extraction fluid reservoir in all other positions it indicates the pressure being supplied to the valve by the regulator Off In this position the CO supply lines are valved off from the system including the extraction fluid reservoir Reservoir vent In this position the CO head pressure is vented from the extraction fluid reservoir to the port labeled Vent 2 1 5 Pressure Gauges Two pressure gauges located below the control knobs display the pressure in the CO cylinder CO supply pressure and in the extraction fluid reservoir Extraction fluid pressure Directly to the left of the extraction fluid pressure gauge is a PSI adjustment screw Using a 7 mm nut driver the gas pressure in the extraction fluid reservoir can be easily adjusted NOTE When the pressure is increased the operator should wait approximately 20 minutes prior to processing a sample This allows for the CO to come into equilibrium with the extraction fluid 2 1 6 Flow Rate Needle Valves Two adjustable flow rate needle valves are located at the top of the hollow fiber concentration cell The left needle valve
21. ting gas to stop and then set the Extraction fluid valve to Fill Load a filter equipped syringe with sterile laboratory grade water and using a section of tubing attach it to the Fill port on the lower right hand side on the front face of the unit Attach a section of tubing to the Vent port on the lower right hand side of the front face of the unit and place the opposite end into a waste container Fill the extraction fluid reservoir with approximately 75 mL of water until fluid begins to flow out of the Vent port Follow Section 3 5 Draining the Extraction Fluid Reservoir to remove the sterile filtered laboratory grade water 3 10 Calibration of the Optical Sensors Warning Before calibrating the optical sensors in the hydrosol concentrator the fluid lines must be clean and dry Any residual liquid or bubbles in the tubing could interfere with accurate calibration of the optical sensors The following procedure should be used to calibrate the optical sensors Press Settings on the home screen Select System settings Select Fluid sensor cal Press Calibrate After the calibration is complete a pass fail page will appear with two light bulbs which corresponds to the two optical sensors An illuminated light bulb indicates an optical sensor did not calibrate correctly If so make sure there is no fluid in the lines then repeat the above procedure Revision 12 27 13 www innovaprep com 9 CHA
22. tory grade water or surfactant solution into the feed reservoir and run as a sample Follow with additional extractions as necessary 4 5 1 Decontamination Fluid Options It is important to check the chemical compatibility of each concentration cell media type before choosing your decontamination fluid e 0 01 N sodium hydroxide run as a sample Follow with multiple rinses of sterile filtered laboratory grade water 200 ppm sodium hypochlorite run as a sample Follow with 200 ppm sodium thiosulfate to deactivate the sodium hypochlorite Follow with sterile filtered laboratory grade water Use chlorine test strips to ensure that the sodium hypochlorite solution has been completely removed Revision 12 27 13 Www innovaprep com 11 e 3 hydrogen peroxide run as sample Follow with multiple rinses of sterile filtered laboratory grade water Use hydrogen peroxide test strips to ensure that the hydrogen peroxide solution has been completely removed 4 5 2 Autoclaving of Concentration Cells The following steam autoclaving method is recommended for use with hollow fiber modules l 2 ER _ Remove and discard all plastic packaging as these should not be autoclaved wet membrane optional Loosen all sanitary connections Luer fittings clamps and flange nuts to avoid damaging the module during the autoclave cycle Wrap all open ports of the connecting tubing with autoclave paper Pre warm the module to 102
23. ut of the vent port it may be necessary to plumb a waste container to catch the foam as the CO off gasses from the remaining extraction fluid After the gas has stopped venting set the Extraction fluid valve to Fill Load a sterile syringe with no more than 50 mL of the desired extraction fluid attach a 0 2 um filter to the syringe and attach the outlet of the filter it to the Fill port Fill the extraction fluid reservoir with no more than 50 mL of extraction fluid Use the volume markings on the syringe to track the volume of extraction fluid loaded into the extraction fluid reservoir Switch the Extraction fluid valve to Run Switch the CO valve to Off Using the 7 mm nut driver slowly turn the Extraction fluid pressure regulator adjustment screw clockwise until the desired pressure is reached NOTE it may take several turns before the regulator engages but once it does engage the pressure can increase rapidly When you are ready to pressurize the extraction fluid switch the CO valve to Run NOTE The operator should wait approximately 20 minutes prior to processing a sample This allows for the CO to come into equilibrium with the extraction fluid Flush the foam line to remove all unpressurized fluid by performing a Foam Line Purge by following the menu path below e Run e Settings e Extract settings Revision 12 27 13 www innovaprep com 7 Foam line purge The LED panel will instruct you to Disconnect cell at port
24. y so that a 100 millisecond extraction produces approximately 100 u L of extraction fluid 8 From this point adjust for your desired sample volume using the Foam Timer setting D 4 3 Concentrating a Liquid Sample 1 Connect a section of tubing from an appropriate reservoir to the Feed In port located on the right hand side of the inside of the instrument 2 Connect a section of tubing from the Perm out port located inside the instrument to a waste container 3 Place a feed sample into the reservoir 4 Place an appropriate sample collection container under the retentate tube on the bottom of the concentration cell or attach the concentrate reservoir and a section of tubing Press Start on the main menu of the LCD Control Panel 6 Sample will be automatically processed and the concentrate will be dispensed into the sample concentrate tube or concentrate reservoir gt 4 4 Additional Sample Extractions Additional extractions of the concentration cell can be performed by pressing 2 on the control panel to emulate a signal from the communication port IN2 4 5 Concentration Cell Decontamination Decontamination of the concentration cell may be necessary in some instances Decontamination can be performed with any of the methods described below e Perform one or more additional extractions of the concentration cell to remove remaining material from the concentration cell e Place clean feed fluid filtered labora
Download Pdf Manuals
Related Search