Complete Protocol
Contents
1. Figure 3 Calibration Run 3500 Data Collection Software as Dashboard Edit Library Maintenance Tools Manage Preferences Help Log Out BE Maintenance 5 Bp Print o Calibrate Calibration Settings Current Instrument Consumables Spatial 2 Polymer Type POP4 Capillary Length 36cm a Number of Wells 96 96 FastTube 384 Chemistry Standard Matrix Standard hd Start Run BFA Performanca Check Plate Position A OB lt p Dr Set Promega 4Dye 7 0 Sequencing Install Standard W Allow Borrowing Bivins Ready Fragment Install Standard HID install Standard Capillary Run Data p Maintenance Wizards Capillary Planned Maintenance Run 1 Notifications Log Run 2 f Run 3 Service Log Dai SENERE B Passed B Failed Me l Not Calibrated assec alec arrowed Jot Calibratec Quality Value Condition Status Message A a Intensity vs Scan Number 7 j Raw Data E EKKA K S iy 0 4000 8000 12000 16000 20000 24000 26000 32000 EA 50000 E5 40000 7 am 30000 7 Z h 20000 10000 5 0 Intensity vs Scan Number Intensity vs Pixel Number lt Accept Reject N oO O 6 TMD048 Printed 10 15 Promega Corporation 2800 Woods Hollow Road Madison WI 53711 5399 USA Toll Free in USA 800 356 9526 608 274 4330 Fax 608 277 2516 www promega com Promega 3 Iffewer than the recommended number of capillaries pass the spectral calibration run may be r2. 4 B Instrument Preparation We have found that the use of fresh polymer and a new capillary array results in an optimal spectral calibration Representative data are shown in Figure 5 File View Service Tools Wizards License 6 Dye Help gt i amp amp elr ROP A A Instrumen Aen aoe oll GA instruments gt 923130x gt DELLIS20BY 1FD gt Spectral Viewer Event Log amp Instrument Protocol W Spatial Calibration Vie E Capillary Viewer W Cap Array Viewer E Spectral Calibration v P Reextraction EJOELLIS2BY 1F0 S2JInstrument Status WhRun Scheduler E Intensity vs Pixel Number WBRun View 4000 a 2000 1000 0 1000 0 1000 2000 3000 4000 5000 Intensity vs Scan Number Capillary Data Thu Aug 20 10 35 56 COT 2015 A01 Dye Set F v Active Calibration for Dye Set F Matrix used for Capillary 15 15 Thu Aug 20 10 35 56 COT 2015 Condition 6 236254 Q Value 0 993085 List of Calibrations for Dye Set F Thu Aug 20 10 35 56 COT 2015 X Override Spectral Rename Figure 5 Representative data for the PowerPlex 4C Matrix Standard on the Applied Biosystems 3130xl Genetic Analyzer using POP 4 polymer and Data Collection Software Version 4 0 1 Set the oven temperature to 60 C and preheat the oven for at least 15 minutes prior to the first injection 2 To perform a spectral calibration for Promega 4 dye STR amplification systems create a new Run Module and Protocol If anew Run Modu
3. TECHNICAL MANUAL PowerPlex 4C Matrix Standard Instructions for Use of Product DG4800 Printed 10 15 TMD048 All technical literature is available at www promega com protocols Visit the web site to verify that you are using the most current version of this Technical Bulletin E mail Promega Technical Services if you have questions on use of this system genetic promega com Bs DVCSCi A OU usages ce xcceses A E A A E A E 1 2 Product Components and Storage Conditions ssccsccsscsscssccsccnscsscsccsccsccescescescesccescescescscescessceses 2 3 Instrument Preparation and Spectral Calibration Using the Applied Biosystems 3500 and 3500xL Genetic Analyzers ssssesseseseesesecsesesscseseoseseosesececseseosesesseseoeoe 2 Orns Malrix Sample F PCO ATAUON sccscentesstcosudiwtecnccsaceacueeuavaneeienueriesissdastesoeadecdanndedecseceovaesteuanwiacacentesiesens 3 Gabbe ANSI UMOCTL PROD ATAIION oy casceycaosavsusceasennmnspancaseessuvaunsuanasnisnaseusctsersuscumsemnuncsenceesteessouustanuxteurucences 3 4 Instrument Preparation and Spectral Calibration Using the ABI PRISM 3100 and 3100 Avant and Applied Biosystems 3130 and 3130xl Genetic Analyzers with Data Collection Software Version 2 0 and Hichi ernia are e aaide 8 Ais Mani Sample Prepara Oi sevscnsonicteecesussteatinkencrtscatoussanescies cous ounsontettecscuseecctavtasevaseanenseesciatecaiuasens 8 AB Insirument Preparation sevcexssesticsserscaccavestesosvou
4. Figure 1 Representative data for the PowerPlex 4C Matrix Standard on the Applied Biosystems 3500xL Genetic Analyzer using POP 4 polymer and Data Collection Software Version 2 0 1 Set the oven temperature to 60 C and then select the Start Pre Heat icon at least 30 minutes prior to the first injection to preheat the oven 2 To perform a spectral calibration for the Promega 4 dye STR amplification systems a new dye set should be created If a new dye set was created previously proceed to Step 2 c a To create this new dye set navigate to the Library highlight Dye Sets and select Create 4 Promega Corporation 2800 Woods Hollow Road Madison WI 53711 5399 USA Toll Free in USA 800 356 9526 608 274 4330 Fax 608 277 2516 TMD048 Printed 10 15 www promega com Promega b The Create New Dye Set window will appear Figure 2 Name the Dye Set e g Promega F select Matrix Standard for the Chemistry and select F Template for the Dye Set Template Select Save Figure 2 The Create New Dye Set window Edit Y Dashboard P Library Maintenance Tools Manage Preferences Help Log Out Library Resources R Manage Plates Assays File Name Conventions Results Group lili Analyze Instrument Protocols Size Standards Bazecalling Protocols Sizecalling Protocols ac Protocols Sequencing Analysis Protocols MicroSeqID Protocols Fragment Analysis
5. Mix to one tube of Matrix Dilution Buffer Vortex for 10 15 seconds Note the date of dilution on the tube Note The diluted 4C Matrix Mix can be stored for up to 1 week at 2 10 C 3 Add 10ul of the diluted 4C Matrix Mix prepared in Step 2 to 500ul of Hi Di formamide Vortex for 10 15 seconds 4 For the ABI PRISM 3100 and Applied Biosystems 3130x Genetic Analyzers 16 wells are used for spectral calibration on 16 capillaries wells Al through H2 of a 96 well plate Add 15ul of 4C Matrix Mix with formamide prepared in Step 3 to each of the 16 wells After placing the septa on the plate briefly centrifuge the plate to remove any air bubbles O Do not heat denature For the ABI PRISM 3100 Avant and Applied Biosystems 3130 Genetic Analyzers four wells are used for spectral calibration on four capillaries wells Al through D1 of a 96 well plate Add 15ul of 4C Matrix Mix with formamide prepared in Step 3 to each of the four wells After placing the septa on the plate briefly centrifuge the plate to remove any air bubbles O Do not heat denature 8 Promega Corporation 2800 Woods Hollow Road Madison WI 53711 5399 USA Toll Free in USA 800 356 9526 608 274 4330 Fax 608 277 2516 TMD048 Printed 10 15 www promega com Promega 5 Place the plate in the 3130 series 96 well standard plate base and cover with the plate retainer Do not start the spectral calibration run until the oven is preheated to 60 C
6. Protocols BH Create A Edit EJ Duplicate g Delete i Import iA Export E Signature 7 View Audit History View E Signature History Capillary Array Serial Number Is Signed m Dye Set Chemistry Standard Calibration Date ex Setup a Dye Set K Dye Set Name Promega 4Dye Locked Chemistry Matrix Standard Md Dye Set Template F Template g lt C Arrange Dyes Dye Selection Reduced Selection Calibration Peak Order 4 Parameters The parameters will be used for instruments configured with 36cm capillary array and polymer POP4 Matrix Condition Number Upper Limit 8 5 Locate Start Point After Scan 750 BeforeScan 5000 Limit ScansTo 2500 Sensitivity 04 Minimum Quality Score 0 95 9322TA Promega Corporation 2800 Woods Hollow Road Madison WI 53711 5399 USA Toll Free in USA 800 356 9526 608 274 4330 Fax 608 277 2516 5 www promega com TMD048 Printed 10 15 O Promega 3 B Instrument Preparation continued c To perform the spectral calibration go to the Maintenance tab select Spectral and under the Calibration Run tab choose the appropriate fields Choose Matrix Standard from the Chemistry Standard drop down menu and the new Promega 4C dye set e g Promega F created in Step 2 b from the Dye Set drop down menu Figure 3 d Select Start Run
7. completely After the first use store the reagents at 2 10 C protected from light Vortex the 4C Matrix Mix for 10 15 seconds prior to use Add 10ul of 4C Matrix Mix to one tube of Matrix Dilution Buffer Vortex for 10 15 seconds Note the date of dilution on the tube Note The diluted 4C Matrix Mix can be stored for up to 1 week at 2 10 C Add 10ul of the diluted 4C Matrix Mix prepared in Step 2 to 500ul of Hi Di formamide Vortex for 10 15 seconds For the Applied Biosystems 3500xL Genetic Analyzer 24 wells are used for spectral calibration on 24 capillaries wells A1 through H3 of a 96 well plate Add 15ul of 4C Matrix Mix with formamide prepared in Step 3 to each of the 24 wells After placing the septa on the plate briefly centrifuge the plate to remove any air bubbles Do not heat denature For the Applied Biosystems 3500 Genetic Analyzer 8 wells are used for spectral calibration on 8 capillaries wells Al through H1 of a 96 well plate Add 15ul of 4C Matrix Mix with formamide prepared in Step 3 to each of the eight wells After placing the septa on the plate briefly centrifuge the plate to remove any air bubbles Do not heat denature Place the plate in the 3500 series 96 well standard plate base and cover with the plate retainer Do not start the spectral calibration run until the oven is preheated to 60 C Instrument Preparation We have found that the use of fresh polymer and a new capillary array resul
8. e a minimum of 750RFU to pass spectral calibration Increase the injection time or voltage Alternatively increase the volume of diluted 4C Matrix Mix added to the Hi Di formamide during matrix sample preparation Peak heights for the matrix standard were too high gt 6 000RFU Decrease the injection time or voltage Alternatively decrease the volume of diluted 4C Matrix Mix added to the Hi Di formamide during matrix sample preparation Peaks in multiple dye channels were detected prior to the red peak Clean the instrument and use fresh polymer fresh buffer and water and new septa Poor quality formamide was used The quality of formamide is critical Use Hi Di formamide Freeze formamide in aliquots at 20 C Multiple freeze thaw cycles or storage at 4 C can cause breakdown of formamide Poor quality formamide can contain ions that compete with DNA during injection which results in lower peak heights and reduced sensitivity For best spectral calibration results use a fresh bottle of polymer fresh buffer and water new septa and a capillary array Check the Event Log in the Instrument Status screen for a reason for the failure e g bad dye order or insufficient number of dye spectra Check the raw data view of the failed capillaries in the Spectral Viewer Look for signs of low signal high signal or baseline noise before the matrix peaks Adjust the run conditions as described above and re inject the ma
9. e compatible with 96 well plates aerosol resistant pipette tips 3500 3500xL capillary array 36cm performance optimized polymer 4 POP 4 for the 3500 or 3500xL anode buffer container with 1X running buffer cathode buffer container with 1X running buffer MicroAmp optical 96 well plate and septa Hi Di formamide Applied Biosystems Cat 4311320 For additional information on performing spectral calibration refer to the Applied Biosystems 3500 3500xL Genetic Analyzer User Guide The quality of formamide is critical Use Hi Di formamide Freeze formamide in aliquots at 20 C Multiple freeze thaw cycles or long term storage at 4 C can cause breakdown of formamide Poor quality formamide can contain ions that compete with DNA during injection which results in lower peak heights and reduced sensitivity A longer injection time may not increase the signal Formamide is an irritant and a teratogen avoid inhalation and contact with skin Read the warning label and take appropriate precautions when handling this substance Always wear gloves and safety glasses when working with formamide 2 Promega Corporation 2800 Woods Hollow Road Madison WI 53711 5399 USA Toll Free in USA 800 356 9526 608 274 4330 Fax 608 277 2516 TMD048 Printed 10 15 www promega com 3 A L ay 3 B Promega Matrix Sample Preparation At the first use thaw the 4C Matrix Mix and Matrix Dilution Buffer
10. e preparation Matrix standard was too concentrated Matrix standard that is too concentrated can result in spectral calibration failure due to saturated peaks bleedthrough or oversubtraction in other dye colors Decrease the volume of diluted 4C Matrix Mix added to the Hi Di formamide during matrix sample preparation Poor quality formamide was used The quality of formamide is critical Use Hi Di formamide Freeze formamide in aliquots at 20 C Multiple freeze thaw cycles or storage at 4 C can cause breakdown of formamide Poor quality formamide can contain ions that compete with DNA during injection which results in lower peak heights and reduced sensitivity Reboot the CE instrument and the instrument s computer Repeat the spectral calibration Ensure that the oven is preheated to 60 C prior to spectral calibration For best spectral calibration results use fresh polymer fresh buffer and water and a capillary array with fewer than 100 injections Promega Corporation 2800 Woods Hollow Road Madison WI 53711 5399 USA Toll Free in USA 800 356 9526 608 274 4330 Fax 608 277 2516 11 www promega com TMD048 Printed 10 15 O Promega 5 B ABI PRISM 3100 and 3100 Avant and Applied Biosystems 3130 and 3130xl Genetic Analyzers Symptoms Causes and Comments Fewer than the recommended number of Peak heights for the matrix standard were too low Matrix peak capillaries passed spectral calibration heights must b
11. epeated automatically up to three times Upon completion of the spectral calibration check the quality of the spectral in the Capillary Run Data display Figure 4 and choose either Accept or Reject not shown Notes 1 Refer to the 3500 Series Data Collection Software HID User Manual for the criteria recommended when accepting or rejecting a spectral calibration 2 Some Applied Biosystems 3500 and 3500xL Genetic Analyzers show imbalance in peak heights e g peaks in the red and yellow dye channels are higher than those in the blue and green dye channels This imbalance should not affect kit performance Capillary Run Data CORSE E A COC S A E E E EE a Passed Bi Failed E Borrowed Not Calibrated Capillary 1 Run 1 Quality Value 0 988753 Condition 5 685121 Status Passed Message q 0 989 5 685 Intensity vs Scan Number Calibrated Data C 23 0 400 800 1200 1600 2000 2400 2800 320 2800 2400 2000 1600 1200 500 400 Intensity vs Scan Number k Intensity vs Pixel Number 9324TA Figure 4 The Capillary Run Data display Promega Corporation 2800 Woods Hollow Road Madison WI 53711 5399 USA Toll Free in USA 800 356 9526 608 274 4330 Fax 608 277 2516 7 www promega com TMD048 Printed 10 15 O Promega 4 Instrument Preparation and Spectral Calibration Using the ABI PRISM 3100 and 3100 Avant and Applied Biosystems 3130 and 3130xl Genetic Analyzers wit
12. h Data Collection Software Version 2 0 and Higher Materials to Be Supplied by the User centrifuge compatible with 96 well plates aerosol resistant pipette tips 3100 or 3130 capillary array 36cm performance optimized polymer 4 POP 4 polymer for the 3100 or 3130 10X genetic analyzer buffer with EDTA MicroAmp optical 96 well plate and septa Hi Di formamide Applied Biosystems Cat 4311320 For additional information on performing spectral calibration refer to the Applied Biosystems 3130 3130xl Genetic Analyzer User Guide The quality of formamide is critical Use Hi Di formamide Freeze formamide in aliquots at 20 C Multiple freeze thaw cycles or long term storage at 4 C can cause breakdown of formamide Poor quality formamide can contain ions that compete with DNA during injection which results in lower peak heights and reduced sensitivity A longer injection time may not increase the signal Formamide is an irritant and a teratogen avoid inhalation and contact with skin Read the warning label and take appropriate precautions when handling this substance Always wear gloves and safety glasses when working with formamide 4 A Matrix Sample Preparation 1 At the first use thaw the 4C Matrix Mix and Matrix Dilution Buffer completely After the first use store the reagents at 2 10 C protected from light 2 Vortex the 4C Matrix Mix for 10 15 seconds prior to use Add 10ul of 4C Matrix
13. ion of a new matrix Individual laboratories should determine the frequency of matrix generation Protocols to operate the fluorescence detection instruments should be obtained from the manufacturer Promega Corporation 2800 Woods Hollow Road Madison WI 53711 5399 USA Toll Free in USA 800 356 9526 608 274 4330 Fax 608 277 2516 1 www promega com TMD048 Printed 10 15 O Promega 2 Product Components and Storage Conditions PRODUCT SIZE CAT PowerPlex 4C Matrix Standard 5 preps DG4800 Not For Medical Diagnostic Use Includes 150ul 4C Matrix Mix e 5x 200ul Matrix Dilution Buffer Storage Conditions Upon receipt store all components at 30 C to 10 C in a nonfrost free freezer protected from light Do not store reagents in the freezer door where the temperature can fluctuate After the first use store the PowerPlex 4C Matrix Standard components at 2 10 C protected from light Do not refreeze the PowerPlex 4C Matrix Standard components We strongly recommend that you store the PowerPlex 4C Matrix Standard with the post amplification reagents The PowerPlex 4C Matrix Standard is light sensitive dilute the 4C Matrix Mix in the Matrix Dilution Buffer in the provided amber tube Store the diluted 4C Matrix Mix at 2 10 C for up to 1 week 3 Instrument Preparation and Spectral Calibration Using the Applied Biosystems 3500 and 3500xL Genetic Analyzers Materials to Be Supplied by the User centrifug
14. le and Protocol were created previously proceed to Step 3 a Inthe Module Manager select New Select Spectral in the Type drop down list and select Spect36_POP4 in the Template drop down list Confirm or change the following settings Injection Voltage 2kV Injection Time 10 seconds Data Delay Time 100 seconds Run Time 800 seconds Note There can be instrument to instrument variation in the sensitivity of detection You may need to adjust injection time or voltage to achieve a passing spectral calibration Peak heights in the range of 1 000 4 000RFU are ideal Peak heights above 750RFU and below the saturation point of the instrument are required Promega Corporation 2800 Woods Hollow Road Madison WI 53711 5399 USA Toll Free in USA 800 356 9526 608 274 4330 Fax 608 277 2516 9 www promega com TMD048 Printed 10 15 O Promega 4 B Instrument Preparation continued b Name the Run Module e g Promega F and select OR c In the Protocol Manager under Instrument Protocols select New Type a name for your protocol e g Promega F d Make the following selections in the Protocol Editor e Spectral in the Type drop down list F in the Dye Set drop down list e POP4 for the polymer e 36 in the Array Length drop down list e Matrix Standard in the Chemistry drop down list e Select the spectral module you created in the previous step in the Run Module dr
15. op down list Finally select Edit Parameters and make the following modifications e Change the lower condition bound to 4 0 and change the upper condition bound to 12 0 e Confirm that the Minimum Quality Score Q value is 0 95 Select OK in the Edit Parameters window and select OK in the Protocol Editor Note The condition number C value obtained when generating a spectral calibration will vary with the instrument After obtaining a spectral calibration that performs acceptably the condition bounds range in the previous step can be narrowed to more critically evaluate C values for subsequent spectral calibrations 3 Inthe Plate Manager create a new plate record as described in the instrument user s manual In the dialog box that appears select Spectral Calibration in the Application drop down list and select 96 well as the plate type Add entries in the owner and operator windows name the plate and select OK 4 In the Spectral Calibration Plate Editor dialog box record sample names in the appropriate cells 5 Inthe Instrument Protocol column select the protocol you created in Step 2 Ensure that this information is present for each row that contains a sample name Select OK 6 Run your plate as described in the instrument user s manual 7 Upon completion of the run check the status of the spectral calibration in the Event Log window For the ABI PRISM 3100 and Ap
16. plied Biosystems 3130x Genetic Analyzers we recommend that a minimum of 12 of 16 capillaries pass calibration For the ABI PRISM 3100 Avant and Applied Biosystems 3130 Genetic Analyzers we recommend that a minimum of three of four capillaries pass calibration If fewer than the recommended numbers of capillaries pass repeat the spectral calibration Note The same plate of matrix standards can be re injected up to four times To re inject the same matrix standards plate add an injection by selecting Plate Manager and then Edit Select Edit again in the top left corner of the window and then select Add sample run 10 Promega Corporation 2800 Woods Hollow Road Madison WI 53711 5399 USA Toll Free in USA 800 356 9526 608 274 4330 Fax 608 277 2516 TMD048 Printed 10 15 www promega com 5 Troubleshooting For questions not addressed here please contact your local Promega Branch Office or Distributor Contact information available at www promega com E mail genetic promega com 5 A Applied Biosystems 3500 and 3500xL Genetic Analyzers Symptoms Causes and Comments Fewer than the recommended number of Matrix standard was too dilute Matrix standard that is too dilute capillaries passed the spectral calibration will result in low spectral calibration peak heights which can result in spectral calibration failure Increase the volume of diluted 4C Matrix Mix added to the Hi Di formamide during sampl
17. rks of Applera Corporation POP 4 is a registered trademark of Life Technologies Corporation Products may be covered by pending or issued patents or may have certain limitations Please visit our Web site for more information All prices and specifications are subject to change without prior notice Product claims are subject to change Please contact Promega Technical Services or access the Promega online catalog for the most up to date information on Promega products Promega Corporation 2800 Woods Hollow Road Madison WI 53711 5399 USA Toll Free in USA 800 356 9526 608 274 4330 Fax 608 277 2516 13 www promega com TMD048 Printed 10 15
18. ssnseancdstds erR EVEA ETEK NENEN 9 S Troubles hoo IIS eins R E E EE TA I A 11 5 A Applied Biosystems 3500 and 3500xL Genetic Analyzers ssesesessesessesesecsesecsscsesecseseosesesecseseose 11 5 B ABI PRISM 3100 and 3100 Avant and Applied Biosystems 3130 and 3130xl Genetic Analyzers 12 Relned POG oercniarn inn r a E E EEEE E NEEE O EE E E A 13 1 Description Proper generation of a spectral calibration file is critical to evaluate multicolor STR data on multicapillary electrophoresis instruments The PowerPlex 4C Matrix Standard consists of DNA fragments labeled with four different fluorescent dyes fluorescein JOE TMR and CXR in one tube The spectral calibration is performed using the F dye set Once generated the spectral calibration file is applied during sample detection to calculate the spectral overlap between the four different dye colors and separate the raw fluorescent signals into individual dye signals The PowerPlex 4C Matrix Standard can be used with any of the 4 dye Promega STR amplification systems A spectral calibration must be generated for each individual instrument A new matrix should be run after major maintenance on the system such as changing the laser calibrating or replacing the CCD camera or changing the polymer type or capillary array We also recommend that you generate a new matrix after the instrument is moved to a new location In some instances a software upgrade may necessitate generat
19. trix standard If the cause for failure is unclear after viewing the Event Log and Spectral Viewer monitoring fragment migration in the Capillaries Viewer during spectral calibration run can provide information that will be useful for troubleshooting purposes Re inject the matrix standard and monitor the Capillaries Viewer during the run Note any unusual peak formations or extremely low or high peak heights Based on the information obtained while watching the Capillaries Viewer you may need to adjust the run conditions 12 Promega Corporation 2800 Woods Hollow Road Madison WI 53711 5399 USA Toll Free in USA 800 356 9526 608 274 4330 Fax 608 277 2516 TMD048 Printed 10 15 www promega com Promega 6 Related Products Product Size Cat PowerPlex 16 HS System 100 reactions DC2101 400 reactions DC2100 PowerPlex 16 System 100 reactions DC6531 400 reactions DC6530 PowerPlex CS7 System 100 reactions DC6613 PowerPlex S5 System 100 reactions DC6951 400 reactions DC6950 GenePrint 10 System 50 reactions B9510 MSI Analysis System Version 1 2 100 reactions 50 reaction pairs MD1641 Not for Medical Diagnostic Use For Research Use Only Not for Use in Diagnostic Procedures 2015 Promega Corporation All Rights Reserved GenePrint and PowerPlex are registered trademarks of Promega Corporation ABI PRISM Applied Biosystems and MicroAmp are registered trademarks of Applied Biosystems Hi Di is a tradema
20. ts in an optimal spectral calibration Representative data are shown in Figure 1 Promega Corporation 2800 Woods Hollow Road Madison WI 53711 5399 USA Toll Free in USA 800 356 9526 608 274 4330 Fax 608 277 2516 3 www promega com TMD048 Printed 10 15 O Promega 3 B Instrument Preparation continued LE 3500 Series Data Collection Software 2 Research Use Only mode cael Dashboard Edit v Library Maintenance Tools Manage v s Help v ry g Maintenance iP Export Spectral Calibration Results j View Spectral Calibration Report QQ Prit ep applied AB biosystems a Calibration Run Calibration Information Calibrate Spatial Y Capillary Run Data copay 7 s Jo fho Ju a2 a3 Jas fas 16 a7 Jis a9 2 Ja 22 23 Jz Performance Check Sequencing Install Standard Fragment install Standard ee Se E Passed I Failed Borrowed Not Calibrated Maintenance Wizards Capillary 6 Run 1 Quality Value 0 993753 Condition 5 867684 Message q 0 994 c 5 868 Planned Maintenance ntensity vs Scan Number Notifications Log Calibrated Data v T o m BS 5 Se Service Log 0 400 800 1200 1600 2000 2800 3200 8000 Schedule 7000 Usage Statistics 6000 r 5000 Rae 4000 A 2000 pra 0 Sy Intensity vs Scan Number p 4 v Intensity vs Pixel Number B E O EDA Ber 0 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 230 Intensity vs Pixel Number
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