Leica MZ16 FA – User manual
Contents
1. Microscopy Systems Our expertise in microscopy is the basis for all our solutions for visualization measurement and analysis of microstructures in life sciences and industry With confocal laser technology and image analysis systems we provide three dimensional viewing facilities and offer new solutions for cytogenetics pathology and mate rials sciences Specimen Preparation We provide comprehensive systems and ser vices for clinical histo and cytopathology applications biomedical research and indus trial quality assurance Our product range includes instruments systems and consum ables for tissue infiltration and embedding microtomes and cryostats as well as auto mated stainers and coverslippers Medical Equipment Innovative technologies in our surgical micro scopes offer new therapeutic approaches in microsurgery Semiconductor Equipment Our automated leading edge measurement and inspection systems and our E beam lithography systems make us the first choice supplier for semiconductor manufacturers all over the world euca MICROSYSTEMS Illustrations descriptions and technical data are not binding and may be changed without notice M2 116 5en Leica Microsystems Switzerland Ltd e CH 9435 Heerbrugg 2003 Printed in Switzerland 1X 2003 RDV2. 12 3 1 1 Assembly diagram 13 3 2 Assembly of stereomicroscope with stand 14 3 2 1 Video photo tube 14 3 2 2 UV protection screen 15 3 Light source uses cer ten 15 3 3 1 Lamp housing 106z 15 3 3 2 Supply units ssuuuuuu 15 34 Electrical connections 16 3 5 Equipping the filter changer 16 3 5 1 Filter sets for fluorescence 17 3 5 2 Filter holders without transponders 17 3 5 3 Inserting the filter 3 5 4 Slot for filter slide 4 Operation 4 1 4 2 4 3 4 4 4 5 Functional principle Starting up 0 cece eee eee eee 18 4 2 1 Disconnecting from the power supply 18 Button functions of the Leica MZ16 FA 19 4 3 1 Menu selection OPT Motorized functions 0005 20 Manual controls 00000 20 4 5 1 Changing button assignments 21 4 5 2 Zoom changing magnification 21 45 3 Focusing sssssessseess 21 4 5 4 Filter change ssssse 21 4 5 5 JV shutter 2 oie ker tren 22 4 5 6 Double iris diaphragm 22 4 5 7 Storing activating and deleting currentsettings 22 4 5 8 A typical work process 23 4 5 9 Observation without fluorescence 24 4 6 Footswitch ss ea 24 4 7 Dioptric correction ssusuuseesee 25 48 Focusing and observing the specimen 25
3. can cause hazards to personnel can lead to functional disturbances and damaged instruments A Danger due to hot surface This symbol warns against touching hot surfaces e g those of light bulbs e I Important information This symbol indicates additional information or explanations that intend to provide clarity Action gt This symbol refers to actions described in the text that are to be carried out Additional notes This symbol indicates additional notes and explanations provided in the text 1 2 Safety instructions Description The Leica MZ16 FA is a motorized fluorescence stereomicroscope with 16 1 zoom digital display and automatic functions for fluorescence applica tions The optics system consists of 2 observation beam paths a patented TripleBeam third beam path specifically for fluorescence illumination and the patented FLUOIII filter system The entire imaging system including the 16 1 zoom objective and ErgoTube is apochromatically corrected Motorized zoom filter changer shutter and double iris diaphragm are motorized They can be con trolled manually and their positions can be stored The Leica MZ16 FA consists of these modules Motorized stereomicroscope Stand binocular tube objective eyepieces and if applicable accessories for photography etc Power supply Filter sets with barrier and excitation filters on one filter holder with transponder
4. Leica MZ16 FA User manual Sica MICROSYSTEMS Dear User Dear User We thank you for your faith in our product and hope it brings you both enjoyment and success With the new Leica MZ16 FA from Leica Micro systems you have purchased the best fluores cence stereomicroscope on the market Discover how you will automatically gain more information faster using the world s first motorized auto mated fully apochromatic fluorescence stereo microscope The Leica MZ16 FA controls the filter changer zoom focus UV shutter and double iris diaphragm at the touch of a button and replicates your multifluorescence experiments very quickly exactly and ergonomically The Leica MZ16 FA is the highest performance fluorescence stereo microscope with the greatest zoom 16 1 the highest resolution 840 Lp mm the highest magni fication 115x with standard optics a patented illumination and filter system for the most intense fluorescence and the HL RC high performance transmitted light base for excellent contrast In developing our instruments we place great value on simple self explanatory operation However please take the time to read the user manual and the operating safety information to become familiar with the advantages and options of your Leica MZ16 FA high performance stereo microscope so that you can use it optimally and safely Should you ever have any questions please consult your local Leica represen
5. Leica MZ16 FA Use 4 8 Focussing and observing the specimen A Follow the safety instructions After switching on the supply unit adjust the burner immediately User Manual M2 216 1 For fluorescence observation always position the aperture protection correctly to protect the observer page 15 A In the filter changer all four positions must be equipped with filter holders The active filter set is the one with its label visible on the left side of the slide slot gt Switch on the supply unit Wait two to three minutes User Manual M2 216 1 gt If you have not done so already adjust the burner as described in User Manual M2 216 1 gt Rotate the desired filter into the beam path using the Fbuttons on the manual controls I Automatic filter detection on the filter hold ers with fluorescence filters there is a transpon der with the specific filter data The Leica MZ16 FA system recognizes whether a fluorescence filter is in position and opens the UV shutter accordingly If the system recognizes a filter holder without transponder the UV shutter remains closed gt Use the Z buttons to adjust to the lowest level of magnification This provides a better overview and allows you to find areas that are of interest more easily gt Use the Fo buttons to focus and use the focus wheel FW to fine focus gt Use the Z buttons to select a higher magnifica tion or to switch to the highest magnification to
6. Numerical aperture 0 28 Field of view 59mm Optical accessories Objective Planapochromatic 1x 2x 1 6x 0 63x lead free Planachromatic 1 0x 0 5x 0 8x with long focal length lead free Working distances 55mm Planapo 1x 19mm Planapo 1 6x 15mm Planapo 2x 97mm Planapo 0 63x 60mm Plan 1x 112mm Plan 0 8x 135mm Plan 0 5x Binocular tube ergonomic Apochromatic ErgoTube 10 50 with synchronous interpupil lary distance adjustment various ErgoModules optional ErgoTube and ErgoModule are registered with the United States Patent and Trademark Office Eyebase interpupillary distance 55mm 75mm Wide field eyepieces for persons 10x 16x 25x 40x distortion free wearing glasses plug on eyecups for protection from infection 32 Leica MZ16 FA Annex Stands illuminators Transmitted light Stand HL RC high performance base with innovative contrast methods stands for bright field and bright dark field Incident light Stand With stage plate black white Stand for large objects Swinging arm stand stage clamp stand universal stand Motorized focus With 300mm or 500mm column control via hand switch foot switch or PC Manual focusing drive Coarse fine focus adjustable ease of movement with 300mm or 500mm column Stages Leica MATS heating stage thermocontrol system gliding stage polarization Illuminators Cold light
7. Three filter holders without transponder for transmitted light observation and protection from UV light Filter holder for individual filters UV protection screen 106 zlamp housing with 50W or 100W high pressure mercury burner with stray light protection Supply unit Leica MZ16 FA Safety concept Permitted uses With the Leica MZ16 FA fluorescence stereo microscope entire fluorescent specimens can be examined three dimensionally and unprepared manipulated sorted and documented The sepa rate beam path for the fluorescence illumination in the Leica MZ16 FA as well as the very bright high pressure mercury burner and specially selected filter sets enable you to distinguish the finest structures and enhance the quantity of information for incident light fluorescence Spe cial filter holders also enable observation without fluorescence in transmitted light or incident light Non intended use If the Leica MZ16 FA its components and acces sories are used other than as described in the user manual injury or property damage may occur Never Install other plugs or cables Change rebuild or take apart parts if not specifically instructed to in this in the manual Allow unauthorized persons to open parts Use the MZ16 FA for examining or operating on the human eye The devices and accessories described in this operating manual have been tested for safety and potential hazards The
8. 48 1 Reflexes useueuueuese 26 49 Calibrating the motorized zoom 26 4 10 Me suring 552 nx RIDERE 28 4 10 1 Selecting the reference circle and unit of measure 28 4 10 2 Measuring sseeeeesee 28 b Anlex x c ER ER io ERE 5 1 FIItORSets 2 odo 0a Soeben 29 5 2 Electrical connection diagrams 30 53 Technicaldata ususuuee 31 5 4 Opticaldata lssssseseseseesse 39 Leica MZ16 FA User manual 1 Safety concept 1 1 The user manual Included with your Leica MZ16 FA fluorescence stereomicroscope is an interactive CD ROM with all pertinent user instructions in German English French Spanish Italian Portuguese Dutch Danish Swedish Finnish and Greek Keep it in a safe place and readily accessible to the user User manuals and updates are also available for you to download and print from our web site at www stereomicroscopy com The Leica MZ16 FA is the motorized and auto mated fluorescence stereomicroscope of the Leica M stereomicroscope series This operating manual describes the special functions of the Leica MZ16 FA and contains important instruc tions for its operational safety maintenance and accessories Like all instruments of the Leica M stereomicroscope series the Leica MZ16 FA con sists of modules which allow an almost unlimited variety of custom equipment configurations The identical modules such as
9. 26 After confirming the selection using the SEL button the following appears in the display in the example CD 1 418 CD start of procedure gt Look into the eyepieces gt Focus the stage micrometer gt Adjust the magnification using the ZW hand wheel on the manual controls exactly so that the diameter of the small circle inside line matches the distance of 1 40 on the stage micrometer gt Confirm and display the calibration press the CAL button twice beeps e Inthe display appears in the example A 50 A mark of zoom position at which calibration was performed Clearing the calibration Press the CAL key at any zoom position Display depending on the mode selected dist or C magn gt Press the SEL button Display according to the mode selected CD XXX or CM XXX gt Press the CAL button for 2 seconds 2 beeps e To indicate that the calibration was cleared the A in front of the magnification is cleared 27 4 10 Measuring With the help of the stage micrometer in the spec imen plane and the graticule 10447182 with two reference circles in the eyepiece you can per form exact measurements To insert the graticule see User Manual M2 105 0 As the reference circle for measurements you can select either the large or small reference cir cle on the graticule in the eyepiece or the com plete field of view The selected reference circle remains stored until itis cleared or rep
10. 4 5 8 A typical work process might be as follows 1 First you want to examine your specimen in overview in bright field transmitted light gt Use the zoom keys Zto switch to the lowest level of magnification and then use the zoom wheel ZWto adjust the magnification to exactly the level you desire gt Rotate the filter changer using the Fkeys until the filter holder without transponder for transmitted or incident light observation without fluores cence is positioned in the beam path gt Focus using the focusing buttons Fo and make fine adjustments using the focus wheel FW gt As necessary adjust the depth of field of the double iris diaphragm using the DI wheel gt To store all positions Press the Set button for approx two seconds until a short signal is heard A For fluorescence always position the aperture protection correctly to protect the observer page 15 2 You now want to change to fluorescence obser vation while maintaining the same zoom setting gt Rotate the filter changer using the F buttons until the desired fluorescence filter is positioned in the beam path gt As necessary make fine adjustments to the focus level using the focus wheel FW and to the double iris diaphragm using the DI wheel gt To store the new positions Press the Set button for approx two seconds until a short signal is heard 3 You now want to change to a high level of magni fication while maintaining the same zo
11. A Do not yet connect the stereomicroscope and peripherals to the power supply Focusing drive gt stand base gt Fasten the manual or motorized focusing drive 4 2 to the column according to User Manual M2 105 0 manual or M2 267 1 motorized on the base 4 1 Tool Allen key gt Fasten the focusing stop 4 3 using the clamp ing screw on the stand column under the focusing drive A When using the motorized focus be certain to read the corresponding User Manual M2 267 1 in particular the safety instructions Microscope carrier gt focusing drive gt Fasten the microscope carrier 4 4 to the Leica MZ16 FA according to User Manual M2 105 0 on the focusing drive 4 2 Tool Allen key Optics carrier gt microscope carrier A The Leica MZ16 FA optics carrier and the FLUOIII filter system make up a single unit which is factory adjusted They may not be taken apart gt Insert the Leica MZ16 FA optics carrier 4 5 according to User Manual M2 105 0 into the microscope carrier 4 4 and fasten it using a clamping screw Additional components gt Fasten the other components and other individ ual accessories according to User Manual M2 105 0 Insert the binocular tube 2 8 into the ring connector on the FLUOIII filter system and fasten it using the clamping screw 14 Insert the eyepieces 4 9 in the tubes and fasten them using the clamping screws If necessary attach soft eyecups see User M
12. Display objective magnification eyepiece magnification video objective total magnifi cation field of view diameter measurements 3 Light emitting diodes aperture of the double iris diaphragm 4 4 Motorized functions The motorized zoom focus filter changer shutter and double iris diaphragm allow ergonomic work using the manual controls The store button allows you to store five combinations of zoom focus filter changer and double iris diaphragm and to replicate experiments quickly and exactly Compared to a manual stereomicroscope the automated and motorized Leica MZ16 FA offers Improved ergonomics Uniform and simple operation More flexibility for the user Free hands for manipulation tasks Faster replication of repetitive tasks If desired either zoom shutter or filter changer can be operated using the footswitch This frees both hands for manipulation sorting and preparation 4 5 Manual controls The manual controls Fig 9 can be arranged as desired at the workplace ideal for both left and right handers The touch free settings of the Leica MZ16 FA prevent vibrations which can cause distortion particularly at high magnification The manual controls are configured ergonomi cally and the outer edge is illuminated so that it is also visible in darkness Every user can store individual functions of the buttons and dials using a PC and recall them using a password see Manual M2 216 3 O
13. GFP2 GFP Plants 470 40nm 450 490nm 525 50nm 500 550nm GFP3 UV 360 40nm 340 380nm 420nm UV Violet 425 40nm 405 445nm 460nm V Blue 470 40nm 450 490nm 515nm B Green 546 10nm 541 551nm 590nm G CHR 436 20nm 426 446nm 480 40nm 460 500nm CFP YFP 510 20nm 500 520nm 560 40nm 540 580nm YFP Texas Red 560 40nm 540 580nm 610 LP nm TXR DsRED 545 12nm 510 560nm 620 60nm 590 650nm DSR Leica MZ16 FA Annex 29 5 2 Electrical connection diagrams Configuration Leica MZ16 FA with manual controls ra pak eet LEICA MZ16 FA Power Supply HANDCONTROL Configuration Leica MZ16 FA with manual controls and PC CTL2 CTL2 CTLI PWR LEICA MZ16 FA 30 POWER SUPPLY HANDCONTROL Configuration Leica MZ16 FA with manual controlsand motorized focus wae HANDCONTROL b LEICA MZ16 FA Configuration Leica MZ16 FA with manual controls motorized focus footswitch and PC IANDCONTROL CTL2 cuz cri PWA DE en POWER SUPPLY FOOT SWITCH LEICA MZ16 FA Leica MZ16 FA Annex 5 3 Technical data Optics carrier Designation Leica MZ16 FA Microscope type Motorized and automated fluorescence stereomicroscope with apochromatic optics system patented TripleBeam third beam path and patented FLUOIII flu
14. manual controls or the ZW wheel gt Focus the specimen gt Activate the menu using the REF button gt Activate the selection menu using the REF button while the current reference circle is still being displayed gt Observe which reference circle most closely matches the specimen portion you want to measure and select Circle 1 or Circle 20r FOV field of vision accordingly using the Zzoom buttons on the manual controls gt Confirm and display your selection using the SEL button beep Depending on the selection chosen the follow ing is displayed C2 or C1 or FOV and mm or inches or mil gt Select mm inches or mil using the Zzoom buttons on the manual controls gt Confirm and display your selection using the SEL button beep The current magnification is displayed again after three seconds gt Observe the specimen portion you want to measure and set the magnification using the ZW zoom wheel on the manual controls so that the reference circle is completely filled by the specimen portion gt Display the measurement using the REF button The current magnification is displayed again after three seconds gt Return to the unit of measure display using the REF button Leica MZ16 FA Use 5 Annex 5 1 Filter sets Filter sets Excitation filter Barrier filter Designation GFP 425 60nm 395 455nm 480nm GFP1 GFP Plus 480 40nm 460 500nm 510nm
15. positions of the rapid filter changer prevent direct UV radiation from reaching the eyes UV filters in the observation beam path protect the eyes The stray light protection on the lamp housing prevents irradiation of the hands A Warning U T V radiation can harm the eyes herefore Never look into the light spot on the specimen plane without a UV protection screen Always fill all of the filter positions with filter holders Fill the empty filter positions with filter holders without transponders Do not select a white strongly reflective background for the stage Never open the lamp housing while the lamp is switched on Risk of explosion UV exposure blinding Supply unit Always unplug the supply unit from the power S upply When installing and disassembling the lamp housing Before opening the lamp housing When replacing the high pressure mercury burner and other parts such as the heat absorbing filter or the collector During maintenance work on the supply unit Leica MZ16 FA Safety concept Lamp housing The Leica MZ16 FA may be used only together with the Leica 106 z lamp housing for the 100W or 50W mercury burner supply unit and stray light protection A Leica Microsystems accepts no liability for damages resulting from the use of lamp housings from other manufacturers Never open the lamp housing while the lamp is switched on Unplug the supply unit from th
16. preset at the factory to control the zoom You can change the functions by operating the footswitch when switching on the system gt The zoom control function on the footswitch remains active if you switch on the system without pressing the footswitch gt The shutter control function is activated if you press the footswitch while switching on the system gt If you want to use the footswitch to control the filter changer switch the system off again and then on and press the footswitch while switch ing the system on The last selected function on the footswitch remains active if you switch on the system without activating the footswitch Leica MZ16 FA Use 4 7 Dioptric correction A Please refer to User Manual M1 105 0 for adjusting the individual settings of the Leica MZ16 FA such as interpupillary distance eye lenses eyepoint eyecups viewing angle etc I Perform dioptric correction in transmitted light or incident light gt Use the Fbuttons on the manual controls to rotate the filter holder without transponder and with closed illumination beam path into the beam path page 21 The shutter closes automatically gt Open the double iris diaphragm completely using the wheel DI on the manual controls all LEDs are lit up gt Illuminate the flat object using transmitted light or oblique incident illumination gt Perform the dioptric correction in accordance with User Manual M1 105 0
17. stands binocular tubes accessories etc are described in User Manual M2 105 0 for Leica M stereomicroscopes which also contains additional safety instructions pertaining to the stereomicroscope accessories and electrical accessories as well as care instructions Special manuals are provided for a number of accessories User Manual M2 216 1 for lamp housing with high pressure mercury burner User Manual for ebq 100 isolated supply unit for 100W high pressure mercury burners User Manual M2 267 1 for the motorized focus system Leica MZ16 FA Safety concept A Before installing operating or using the instruments read the user manuals listed above In particular please follow all safety instructions To maintain the unit in its original condition and to ensure safe operation the user must follow the instructions and warnings contained in these user manuals 1 1 1 Diagrams 1 2 Numbers in parentheses within the descrip tions pertain to diagrams and the items in the dia grams Example 1 2 Figure 1 is located on page 3 and item 2 is the filter changer 1 1 2 Symbols used A Warning of a danger This symbol indicates especially important information that if not observed can cause hazards to personnel can lead to functional disturbances and damaged instruments A Warning of hazardous electrical voltage This symbol indicates especially important infor mation that if not observed
18. 5 8 2 27 3 10 2 22 20 5 10 9 25 6 8 8 1 16 14 8 28 10 2 22 12 8 17 5 25 6 8 8 32 7 1 25 20 11 2 10 22 4 12 8 175 16 14 32 7 40 56 1 6 25 6 8 8 12 8 175 16 4 13 7 20 5 10 9 41 5 5 51 2 44 2 32 7 16 14 20 5 10 9 25 6 8 8 51 2 44 64 35 25 40 5 6 20 11 2 25 6 8 8 32 7 64 35 80 28 16x 14B 3 2 51 2 44 25 6 8 8 32 8 6 8 4 55 81 9 27 102 4 22 4 64 35 32 7 4 5 5 51 2 44 102 4 2 2 128 1 8 5 80 28 40 5 6 51 2 44 64 35 128 1 8 160 1 4 6 3 100 8 22 50 4 4 4 64 5 35 80 6 2 8 161 3 1 4 201 6 14 8 128 18 64 35 81 9 27 102 4 2 2 204 8 14 256 0 9 10 160 14 80 28 102 4 22 128 18 256 09 320 07 115 184 12 92 24 115 9 19 1472 15 294 4 08 368 06 0 71 177 13 4 8 9 26 8 11 1 21 2 14 2 16 7 28 3 8 4 35 4 67 0 8 20 119 10 238 128 18 6 16 148 32 74 40 59 1 25 95 125 19 16 14 8 20 11 9 40 5 9 50 48 1 25 31 3 76 15 6 152 20 119 25 95 50 48 62 5 38 1 6 40 59 20 119 25 6 93 32 74 64 37 80 3 2 50 48 25 95 32 74 40 59 80 3 100 24 25 62 5 38 313 16 40 59 50 48 100 24 125 19 25x 9 5B 3 2 80 3 40 59 51 2 46 64 37 128 1 9 160 15 4 100 24 50 48 64 37 80 3 160 15 200 12 5 125 19 62 5 38 80 3 100 24 200 12 250 1 63 157 5 15 78 8 3 100 8 24 126 19 252 0 9 315 0 8 8 200 12 100 24 128 19 160 15 320 07 400 0 6 10 250 1 125 19 160 15 200 12 400 0 6 500 05 115 287 5 08 143 8 17 181 1 13 230 1 460 05 575 04 0 71 28 4 8 4 14 2 16 9 119 13 4 22 7 10 5 45 4 5 3 56 8 42 0 8 32 75 16 15 20 5 117 25 6 9 4 51 2 47 64 3 8 1 40 6 20 12 25 6 94 3 15 64 38 80 3 1 25 50 48 25 96 32 75 40 6 80 3 100 24 1
19. 6 64 38 32 75 a 59 51 2 47 102 4 23 128 19 2 80 3 40 6 51 2 41 64 38 128 1 9 160 15 40x 6B 25 100 24 50 48 64 3 8 80 3 160 15 200 12 32 128 19 64 38 81 9 29 1024 23 204 8 12 256 09 4 160 15 80 3 102 4 23 128 19 256 0 9 320 0 8 5 200 12 100 24 128 19 160 15 320 0 8 400 0 6 6 3 252 1 126 19 161 3 15 201 6 12 403 2 0 6 504 05 8 320 08 160 15 204 8 12 256 0 9 512 05 640 04 10 400 0 6 200 12 256 0 9 320 0 8 640 04 800 03 115 460 05 230 1 289 8 08 368 0 6 736 0 3 920 0 3 Leica MZ16 FA Annex 39 Leica Microsystems the brand for outstanding products Leica Microsystems mission is to be the world s first choice provider of innovative solutions to our customers needs for vision measurement lithography and analysis of microstructures Leica the leading brand for microscopes and scientific instruments developed from five brand names all with a long tradition Wild Leitz Reichert Jung and Cambridge Instruments Yet Leica symbolizes innovation as well as tradition Leica Microsystems an international company with a strong network of customer services Australia Gladesville NSW Tel 1 800 625 286 Fax 61 2 9817 8358 Austria Vienna Tel 43 1486 80500 Fax 43 1 486 80 50 30 Canada Richmond Hill Ontario Tel 1 905 762 2000 Fax 1 905 762 89 37 China Hong Kong Tel 8522 564 6699 Fax 8522 564 4163 Denmark Herlev Tel 45 44 5401 01 Fax 45 44 5401 11 France Rueil Malmaison Cedex Tel 331 4732 8585 Fax 33 1 4732 85
20. 86 Germany Bensheim Tel 49 6251 1360 Fax 49 6251 136 155 Italy Milan Tel 39 02 57 486 1 Fax 39 02 5740 3273 Japan Tokyo Tel 81 3543 596 09 Fax 81 3543 596 15 Korea Seoul Tel 82 2 514 6543 Fax 82 2 514 6548 Netherlands Rijswijk Tel 31 704132130 Fax 31 70 41 32 109 Portugal Lisbon Tel 4351213814766 Fax 35 1 213 854 668 Singapore Tel 65 6 77 97 823 Fax 65 6 77 30 628 Spain Barcelona Tel 34 93 494 9530 Fax 34 93 494 9532 Sweden Sollentuna Tel 46 8 625 45 45 Fax 46 8 625 45 10 Switzerland Glattbrugg Tel 41 44 8093434 Fax 41 44 809 34 44 United Kingdom Milton Keynes Tel 44 1908 246 246 Fax 44 1908 609 992 USA Bannockburn Illinois Tel 1 800 2480123 Fax 1 847 405 0164 and representatives of Leica Microsystems in more than 100 countries In accordance with the ISO 9001 certificate Leica Microsystems Switzerland Ltd Business Unit Stereo amp Macroscope Systems has at its disposal a management system that meets the requirements of the international standard for quality management In addition production meets the requirements of the international standard ISO 14001 for environmental management Leica Microsystems Switzerland Ltd Business Unit SM CH 9435 Heerbrugg Telephone 41 71 726 33 33 Fax 41 71 726 33 99 www leica microsystems com www stereomicroscopy com The companies of the Leica Microsystems Group operate internationally in four business segments where we rank with the market leaders
21. Inorder to prevent damage from vibrations disassemble all moving parts that according to the user manual can be assembled by the cus tomer and pack them separately These include the objective binocular tube eyepieces lamp housing burner in the 106 z lamp housing etc Repairs and servicing Only Leica Microsystems trained service tech nicians or technical specialists assigned by the person in charge of the instrument are permitted to carry out repairs Only original Leica Microsystems spare parts may be used Disconnect the power cable before opening volt age carrying parts such as the Leica MZ16 FA the power supply or the supply unit A Touching the live circuit can cause injury Installation in third party products When installing Leica products into third party products note the following The manufacturer of the complete system or its dealer is responsible for following all applicable safety instructions laws and guidelines Disposal The product must be disposed of in accordance with locally applicable laws and regulations Dispose of used high pressure mercury burn ers as hazardous waste Legal requirements Adhere to general and local regulations relating to accident prevention and environmental protection Conformity with European Community directive The Leica MZ16 FA and its accessories are con structed in accordance with the latest technolo gies and are provided with a sta
22. Sh Shutter for opening closing the illumination beam path only possible in fluorescence mode DI Aperture ofthe double iris diaphragm Additional operating elements see page 3 Leica MZ16 FA Annex 38 5 4 Optical data Objectives eee 0 5x Plan 0 63x Planapo 0 8x Plan 1 6x Planapo 2x Planapo 1x Planapo Working distances in mm a 135 Plan 97 Planapo 112 Plan 19 Planapo 15 Planapo m 55 Planapo Eyepieces E E 3 5 3 3 E 3 g EJ g 3 g 8 g 3 E t E BE t 85 E gE ze E 25 S 25 Ej 25 S 25 Ej 25 25 E ss E et E et E et E et E 63 3 35 8 35 8 38 3 35 8 SE 8 S5 z e uo F irs B io irs B io ins 0 71 71 29 6 35 59 1 45 47 5 7 37 11 4 18 5 14 2 14 8 0 8 8 26 3 4 52 5 5 1 41 2 6 4 32 8 12 8 16 4 16 13 1 1 10 21 5 42 64 32 8 8 26 3 16 13 1 20 10 5 1 25 125 16 8 6 3 33 3 8 26 3 10 21 20 10 5 25 84 1 6 16 13 1 8 26 3 10 2 20 6 128 164 25 6 8 2 32 6 6 2 20 10 5 10 21 12 8 16 4 16 13 1 32 6 6 40 53 25 25 8 4 12 5 16 8 16 13 1 20 10 5 40 53 50 42 10x 21B 3 2 32 6 6 16 13 1 20 5 10 2 25 6 8 2 51 2 41 64 3 3 4 40 5 3 20 10 5 25 6 8 2 32 6 6 64 33 80 26 5 50 42 25 84 32 6 6 40 53 80 2 6 100 21 6 3 63 3 3 31 5 67 40 3 52 504 42 100 8 2 1 126 17 8 80 26 40 53 51 2 41 64 3 3 128 1 6 160 1 3 10 100 2 1 50 42 64 3 3 80 2 6 160 13 200 14 115 115 18 57 5 37 725 29 92 22 184 1 1 230 0 9 071 11 4 197 57 39 4 72 313 91 246 182 123 228 98 08 128 17 5 6 4 3
23. Wzoom wheel is moved ZW Precise zooming using the zoom wheel Leica MZ16 FA Use 4 5 3 Focusing Fo and FW Use the Fo buttons to focus quickly and use the focus wheel FWfor fine and precise up down focusing Fo Focus buttons Constant pressure the motorized focus travels continuously up down FW Precise fine focusing with focus wheel I For fine focusing with the focus wheel FW the system recognizes the level of magnification The higher the magnification the more slowly the motorized focus moves 4 5 4 Filter change F A For observation with fluorescence light always position the aperture protection correctly to protect the observer page 15 Fbuttons Filter changer left right rotation buttons At the touch of a button the filter changer rotates to the left or the right into the next position at maximum speed During this process the shutter automatically closes the illumination beam path to preserve sensitive specimens and protect users from UV radiation The active filter set is always the one with its label visible on the left side of the slide slot The UV shutter opens automatically if a fluorescence filter with transponder is in position and stays closed if a filter holder without transponder is in position 21 4 5 5 UV shutter Sh The UV shutter Sh opens and closes the illumina tion beam path i The UV shutter closes the illumination beam path automatically and canno
24. anual M2 105 0 Screw the objective 4 10 into the bottom of the optics carrier 4 5 counterclockwise 3 2 1 Video photo tube We recommend using the trinocular video photo tube 4 8 or the HU video phototube with 100 light in the video photo output in order to achieve shorter exposure times at low fluorescence levels gt Insert the video photo tube 4 8 into the ring connector on the FLUOIII filter system accord ing to User Manual M2 105 0 and fasten it using the clamping screw gt Fasten a binocular tube to the HU video photo tube using a clamping screw Fig 4 Right side of the Leica MZ16 FA Leica MZ16 FA Assembly 3 2 2 UV protection screen gt microscope carrier gt Fasten the UV protection screen 5 1 using a hexagon head screw 5 3 to the left or right side of the microscope carrier Tool Allen key A Always position the UV protection screen so that the operator can never look directly at the light spot see page 9 gt Set the UV protection screen 5 1 laterally using the arm 5 2 gt Loosen the hexagon head screw 5 3 gt Move the UV protection screen up or down using the arm gt Tighten the hexagon head screw 5 3 Fig 5 UV protection screen 1 UV protection screen 2 Arm 3 Hexagon head screw Leica MZ16 FA Assembly 3 3 Light source The Leica 106 z lamp housing is the light source for the Leica MZ16 FA and is used with 100W or 50W mercury lamps corr
25. be absolutely cer tain to insert the included filter holders without transponders with closed illumination beam path into the free positions Otherwise there is a risk of danger to the eyes from direct UV radiation from the third beam path Also they protect the system from dust Fig 6 Rear side of Leica MZ16 FA with connec tions to power supply motorized focus manual controls footswitch PC 16 Fig 7 Filter changer equipped with filter holders 1 Filter sets 2 Filter slide for filter holder with individual filters Leica MZ16 FA Assembly 3 5 1 Filter sets for fluorescence The filter sets for fluorescence contain on one filter holder two barrier filters to the visual beam paths and one excitation filter to the illumination beam path A transponder is located on the fluo rescence filters with the specific filter data The transponder guarantees dependable automatic operation with filter recognition The filter sets are labeled see page 28 I Please order Leica filter sets for fluores cence from Leica Microsystems The transpon ders on the filter sets are required to ensure proper function of the automatic filter detection 3 5 2 Filter holders without transponders Three filter holders without transponders are included in the scope of delivery of the Leica MZ16 FA These filter holders have two empty openings for the observation beam paths The third opening to the illumination beam path i
26. ched on the burner must be adjusted immediately First work in transmitted light mode in order to familiarize yourself with the microscope s controls A Follow the safety instructions gt Attach the power supply to an outlet All motorized microscope components first undergo an initialization phase The display indi cates init V1 xx software version e g SW V1 xx The unit is being initialized beeps MZ16 FA appears quickly to indicate that initialization is finished beeps twice Display of the current magnification Factory settings Objective 1 0x Eyepiece 10x Video objective 0 63x CAL C dist reference distance REF FOV field of view diameter Acoustic signal on 4 2 1 Disconnecting from the power supply A Unplug the power cable from the power supply Status when unplugging the power cable The selection under OPT the calibration and the diameters of the reference circles remain the same The positions stored using the manual control system Set button are cleared The last selected function on the footswitch remains active if you switch on the system without activating the footswitch Leica MZ16 FA Use 4 3 Button functions of the Leica MZ16 FA In the menu the Z up down zoom buttons on the manual control system assume the function of selection keys see Fig 9 OPTic Select eyepiece and objective factors Select the factor Acoustic signal on off SELect Confirm a
27. e and Leica DC500 digital camera 7 Filter holder for an individually selectable filter 12 Leica 106 z lamp housing for 50W or 100W such as a gray filter mercury burner with stray light protection 8 ErgoTube 2 8 or trinocular tube 3 8 pictured not pictured with video objective and Leica DC500 digital 13 Supply unit for 106 z lamp housing not pictured camera 14 Manual controls for zoom motorized focus 9 Wide field eyepieces for persons wearing filter changer shutter with connection to optics glasses carrier and or footswitch not pictured 10 Interchangeable objective planachromatic or 15 Transformer not illustrated planapochromatic 16 Gliding stage optional 11 UV protection screen with arm Leica MZ16 FA Overview of the instrument 11 3 Assembly Basic components such as the stand column microscope carrier binocular tubes and optical accessories are thanks to their modular design identical for all Leica M series stereomicro scopes Therefore please refer to User Manual M2 105 0 for Leica M stereomicroscopes for installation and assembly instructions The connections of the manual controls footswitch and PC as well as the assembly of the UV protection screen are described in the user manual you are now reading The lamp housing supply unit and adjustment of the high pressure mercury burner are described in User Manual M2 216 1 12 3 1 Assembly sequence The microscope components are best assemb
28. e power supply Risk of explosion UV exposure blinding Before opening the lamp housing allow it to cool off for at least 15 minutes Danger of explosion danger of burns Never cover the air duct on the lamp housing Danger of fire Use the stray light protection Danger to eyes from UV radiation 2 Overview of the instrument Fig 2 Left side of the Leica MZ16 FA with ErgoTube from the user s perspective 1 Stand transmitted or incident light stand with 5 The Leica MZ16 FA with two observation beam corresponding illumination Pictured here is paths and a TripleBeam third beam path the HL RC transmitted light base for best motorized FLUOIII filter system and adapter for relief contrast 106 z lamp housing 2 Focusing drive motorized focus system with 6 Filter sets the scope of delivery includes three column pictured or manual coarse fine drive filter holders without transponders for empty see User Manual M1 105 0 filter positions and transmitted or incident light 3 Focusing stop observation without fluorescence Additionally 4 Microscope carrier filter sets of your choice for fluorescence excitation and barrier filter on one filter holder are available 10 Leica MZ16 FA Overview of the instrument CO 10 11 16 14 Fig 3 Right side of the Leica MZ16 FA from the user s perspective with trinocular tube video objectiv
29. esponding supply units and stray light protection I Please read the separate User Manual M2 216 1 for the Leica lamp housing the user manual and safety instructions for the ebq 100 supply unit the user manual and safety instructions provided by the lamp manufacturer and in particular the section on how to proceed if a lamp breaks and releases mercury A Do not switch on the supply unit until you have read and familiarized yourself with the functions of the Leica MZ16 FA in transmitted light provided in the Operation chapter After it is switched on the burner must be adjusted immediately 3 3 1 Lamp housing 106 z Fasten the 106 z lamp housing 4 12 to the adapter part as described in User Manual M2 216 1 3 3 2 Supply units The supply unit for 50W mercury burners is described in User Manual M2 216 1 The 100W mercury burners are powered by the ebq 100 supply unit Please read the separate instruction manual provided by the manufacturer 3 4 Electrical connections The connections are used differently according to the configuration Diagrams with connections and paths for typical configurations with manual controls motorized focus footswitch and PC are provided on page 29 PWR Connection for Leica MZ16 FA power pack or empty for feed from power pack of motorized focus CTL1 Foot switch connection and or PC interface kit with Y cable CTL2 Connection for manual control
30. filter slide There is a slot 7 2 in the filter changer system for an individually selectable filter for example a gray filter 4 Operation 4 1 Functional principle The most important functions of the Leica MZ16 FA are motorized and automated e Motorized functions zoom motorized focus optional filter changer aperture and double iris diaphragm Automated functions automatic filter detection automatic shutter automatic control of five working procedures calculation of measure ments the current total magnification and the field of view diameter Memory functions zoom motorized focus optional filter changer and double iris diaphragm Variable function keys the microscope is deliv ered with pre assigned functions of the func tion keys of the manual control system and the footswitch These functions can be repro grammed and or adapted to your individual wishes The selector buttons of the Leica MZ16 FA are intended for selecting the objective eyepieces video objective and acoustic signal The data are used when calculating the magnification the field of view diameter and the measure ments The current total magnification field of view diameter and measurements are displayed on screen Light emitting diodes for the aperture of the double iris diaphragm 18 4 2 Starting up A caution Do not switch on the supply unit for the high pressure mercury burner yet After it is swit
31. laced by a different mode After the reference circle has been selected and the baseline adjusted to the reference circle the Leica MZ16 FA displays the measurements at the desired magnification with the selected unit of measure 4 10 1 Selecting the reference circle and unit of measure gt Activate the menu using the REF button The display shows the reference circle C2 or C7 or FOV selected last and the diameter for the current magnification The current magnifi cation is displayed again after three seconds gt Activate the selection menu using the REF button while the current reference circle is still being displayed gt Select Circle 1 or 20r FOV field of view using the Zzoom buttons on the manual controls gt Confirm and display your selection using the SEL button beep Depending on the selection chosen the follow ing is displayed C2 or C1 or FOV and mm or inches or mil gt Select mm inches or mil using the Zzoom buttons on the manual controls gt Confirm and display your selection using the SEL button beep The current magnification is displayed again after three seconds gt Return to the unit of measure display using the REF button 28 4 10 2 Measuring I We recommend using the optional gliding stage for very fine positioning of the specimen for measurement purposes gt Set the magnification you want to use for mea surement exactly using the Zzoom buttons on the
32. led in this order Stand base and column with focusing drive manual or motorized and focus and focusing stop Microscope carrier Leica MZ16 FA optics carrier Binocular or trinocular tube Eyepieces and eyecups Stage plate or special stage gliding stage heating stage Objective UV protection screen with arm with light source Lamps high pressure mercury burner in lamp housing for the HL RC transmitted light stand fiber optic light guide with light source Supply unit for lamp housing Electrical connections Manual controls and or footswitch and PC if applicable Equipment of filter changer Either the integrated clamping screws or an Allen key is necessary for assembly these are included in delivery Leica MZ16 FA Assembly 3 1 1 Assembly diagram 10 447 160 10 445 301 10 445 302 10 445 303 10 446 237 10 446 197 10 445 822 10 446 229 10 446 238 warm 1044620 KES RS 232 l 10447 063 10 446 271 amp 10447220 10 447 221 10 447 217 10 446 182 10 447 222 10 447 218 10 447 157 10 447 223 10 447 219 10 447 051 10 447 224 10 447 050 10 447 225 10 446 157 10 445 819 10 447 226 10 447 227 10 447 101 10 447 075 Plan Planapo m 10 446 301 10 445 631 10 445 363 10 445 387 10 445 367 10 446 359 Leica MZ16 FA Assembly 13 3 2 Assembly of stereomicroscope with stand
33. n the follow ing pages the functions are described according to their preset factory button assignments 20 Fig 9 Manual controls factory preset button assignments Set Store the current positions of zoom focus filter changer double iris diaphragm for five combinations Z Function 1 Zoom up down quick zoom Function 2 menu selection buttons ZW Zoom up down fine zoom Fo Focus up down quick focus FW Focus up down fine focus F Filter changer clockwise counterclock wise rotation Sh Shutter for opening closing the illumination beam path only possible in fluorescence mode DI Aperture of the double iris diaphragm Leica MZ16 FA Use 4 5 1 Changing button assignments Each user can individually select the functions of the buttons and wheels using a PC Multiple users can store their own configurations and recall them using passwords The scope of delivery includes a CD ROM with the configuration program and its description 4 5 2 Zoom change magnification Zand ZW Use the Z buttons to zoom quickly using the zoom wheel ZW for fine and precise movements up and down Z Zoom buttons Constant pressure motorized zoom travels continuously up down Precise fine setting using the ZW wheel One quick click the motorized zoom adjusts to the highest next level of magnification I The speed accelerates to the maximum speed The motorized zoom stops when the Z button is clicked again or the Z
34. nd display the selection CALibration Calibrating the motor zoom REFerence Display diameter of reference circle for measure ments SEL CAL simultaneously Travel to the calibrated zoom position SEL press for approx 2 seconds Reset user settings to factory settings Display def val 4 3 1 Menu selection OPT Selection of eyepiece objective video objective acoustic signal In the menu the Zup down zoom buttons on the manual control system assume the function of selection keys see Fig 9 OPT Select the eyepiece factor Zup down zoom buttons gt Confirm and display the selection SEL beep Leica MZ16 FA Use Select the objective factor Z up down zoom buttons gt Confirm and display the selection SEL beep Select video objective factor Z zoom up down buttons gt Confirm and display the selection SEL beep I This factor does not influence the calculation or display of the magnification or of the measure ments During image capture via digital camera it calculates the magnification of the live image Acoustic signal on off beep Z up down zoom buttons gt Confirm and display the selection SEL beep 3 2 Fig 8 Display with function keys 1 Function keys OPT Selection of eyepiece objective video or photo objective acoustic signal Confirm and display the selection Calibrate motor zoom Display diameter of reference circle for measurements SEL CAL REF 2
35. observe greater details gt As necessary set the magnification exactly using the ZW zoom wheel 25 4 8 1 Reflexes When using the 1 6x planapochromatic objective a light reflex appears between zoom positions 0 8 and 1 6 which disappears at higher zoom settings This reflex does not occur when using the 1 0x and 0 63x planapochromatic objectives 26 4 9 Calibrating the motorized zoom The adjustment accuracy of the motor zoom is factory set to 5 If you want to work more precisely for example while measuring you can calibrate the system The calibration is stored until it is modified or cleared I Always perform the calibration at a ratchet step 50 0 in the example Calibration modes Magnification C magn or reference distance C dist Calibration aids Stage micrometer in the specimen plane and graticule 10447182 in the eyepiece The diameter of the inner circle measures 7mm For more information on inserting the graticule see User Manual M2 105 0 The optional gliding stage facilitates the precise movement of the stage micrometer At Limit If this message appears on the display during calibration of the reference distance with the handwheel you have traveled beyond the adjustable range Initializing calibration Clearing previous calibrations see p 27 gt Move to the desired zoom ratchet step by pressing the Zzoom button on the manual controls in the e
36. om setting gt Use the zoom keys Zto switch to the highest level of magnification or use the zoom wheel ZWto adjust the magnification to exactly the level you desire gt As necessary make fine adjustments to the focus level using the focus wheel FW and to the double iris diaphragm using the DI wheel gt To store the new positions Press the Set button for approx two seconds until a short signal is heard 23 4 5 9 Observation without fluorescence If you want to change to transmitted light or inci dent light observation without fluorescence use a filter holder without transponder with closed illu mination beam path The openings in this filter holder are empty there is no barrier filter in the beam path The color reproduction is genuine Three filter holders without transponders are included in the scope of delivery of the Leica MZ16 FA The Leica MZ16 FA system recognizes that a filter holder without transponder is in position The shutter remains closed to prevent the filter holder from overheating Fig 11 Foot switch 24 4 6 Footswitch As desired either zoom shutter or filter changer can be operated using the footswitch This frees both hands for manipulation sorting and prepara tion Zoom right switch up left switch down Shutter right switch open left switch closed Filter changer right switch clockwise rotation left switch counterclockwise rotation The footswitch is
37. ons for zoom focus filter changer and double iris diaphragm For the next experiment the Leica MZ16 FA automatically configures all settings at the touch of a button Set Store the current positions of zoom focus fil ter changer double iris diaphragm for a maximum of five combinations Store Hold down the button for approx two seconds until a short signal is heard Quickly move to positions press button quickly Clear press button for approx four seconds until a long signal is heard Fig 10 Manual controls factory preset button assignments Leica MZ16 FA Use I You can use the Set store button on the manual controls to store activate or clear the cur rent positions of zoom focus filter changer double iris diaphragm Amaximum of five combinations can be stored e fasixth combination is stored the one nearest to it will be cleared Each combination can be cleared individually The system will travel to each of the individual combinations in the sequence in which they were stored Switching off the system clears the stored combinations Activating the stored positions Quickly move to positions press Set button quickly The Leica MZ16 A automatically sets the stored positions of zoom focus filter changer double iris diaphragm Deleting the stored positions Press the Set button for approx four seconds until a long signal is heard Leica MZ16 FA Use
38. orescence filter system 100 apochromatic optics system CMO design common main objective lead free Motorized functions Zoom filter changer UV shutter double iris diaphragm motor ized focus optional Control system Manual control system foot switch Manual controls illuminated switch positions via PC individually configurable For control of zoom filter changer UV shutter double iris diaphragm motorized coarse and fine settings for zoom and focus focus speed corresponding to zoom the higher the finer Buttons for storing the positions of zoom filter changer double iris diaphragm motorized focus Foot switch For zoom filter changer or UV shutter PC connection Via RS232 interface Display For objective magnification eyepiece magnification total magnification measured values aperture of the double iris diaphragm camera objective Automated functions Absolute or user defined calculation of the total magnification Filter detection Control system for stored functions such as zoom focus filter change double iris diaphragm UV shutter Calibration of measured values when changing zoom Automatic control of multifluorescence experiments Double iris diaphragm for balancing the depth of field Installed motorized Leica MZ16 FA Annex 31 Fluorescence system Illumination beam path TripleBeam 2 observation 1 separa
39. responsible Leica affiliate or the main plant in Wetzlar must be consulted whenever the device is altered modified or used in conjunction with non Leica components that are outside of the scope of this manual Unauthorized alterations to the device or noncom pliant use shall void all rights to any warranty claims Leica MZ16 FA Safety concept Place of use The Leica MZ16 FA may only be operated in a closed dust free room which is free of oil vapors and other chemical vapors as well as extreme humidity and within a temperature range of 10 to 40 Electrical components must be assembled at least 10cm away from the wall and from flam mable substances Large temperature fluctuations direct sunlight and vibrations should be avoided These condi tions can distort measurements and micro graphic images Stereomicroscopes in warm and warm damp climatic zones require special care in order to prevent the build up of fungus Responsibilities of the person s in charge of instrument Ensure that the Leica MZ16 FA and accessories are oper ated maintained and repaired only by autho rized and trained personnel the operating personnel has read understands and is observing these instructions as well as those listed on page 5 and in particular the safety instructions Transport Use the original packaging for shipping or transporting the stereomicroscope and its accessory components
40. ructions provided Column with motorized focus on Resolution 2 microns incident light or transmitted light Input voltage 85 to 264 VAC basis type MST31 Input frequency 47 to 440 Hz Input current max 0 80 A at 115 VAC max 0 50 A at 230 VAC Operating voltage 30V Operating temperature 10 C to 40 C Storage temperature 20 C to 55 C Maximum mechanical loading 12 kg Focusing drive coarse fine maximum load 15 kg 1 micron resolution at 5 kg load 2 micron resolution at 10 kg load 34 Leica MZ16 FA Annex Operating controls Fig 13 Display with function keys 1 Function keys OPT Selection of eyepiece objective video or photo objective acoustic signal SEL Confirm and display the selection CAL Calibrate motor zoom REF Display diameter of reference circle for measurements Fig 12 Manual controls factory preset button assignments 2 Display objective magnification eyepiece magnification video objective total magnifi Set Store the current positions of cation field of view diameter measurements zoom focus filter changer double iris diaphragm for five combinations 3 Light emitting diodes aperture of the double iris diaphragm Z Function 1 Zoom up down quick zoom Function 2 menu selection buttons ZW Zoom up down fine zoom Fo Focus up down quick focus FW Focus up down fine focus F Filter changer clockwise counterclock wise rotation
41. s closed Use these filter holders if you want to change to transmitted light or incident light observation without fluorescence at all times in empty filter changer positions in order to protect yourself from UV radiation and protect the system from dust A If you are using fewer than four filter hold ers with fluorescence filters be absolutely cer tain to insert the included filter holders without transponders with closed illumination beam path into the free positions Otherwise there is a risk of danger to the eyes from direct UV radiation from the third beam path Leica MZ16 FA Assembly 3 5 3 Inserting the filter I When inserting the filter sets avoid touch ing the filters if at all possible in order to avoid fingerprints If you touch them clean the filters using a soft lint free cloth and pure alcohol gt Insert the filter holder 7 1 into the filter changer 2 6 such that the label e g GFP1 is upright and legible on the right side of the slide slot e Ensure that the contour of the filter holder matches the contour of the microscope gt Then connect the power supply to the power so that you can rotate the motorized filter changer gt Rotate the filter changer using the Fkeys on the manual controls until the stop see page 21 fthe filter holder has not been inserted correctly the filter changer does not rotate Insert a total of four filter holders 3 5 4 Slot for
42. s or empty when connected to the second CTL2 socket CTL2 Connection for manual controls or empty when connected to the first CTL2 socket or con nection for short connecting cable for connecting CTL2 to motorized focus A For models equipped with motorized focus be certain to adhere the cable clip to the motor ized focus and insert the short connecting cable Otherwise the cable could be squeezed between the stop and motorized focus during focusing Connection to PC The connection to a PC requires a standard PC with RS232 interface and a Leica PC Interface Kit The individual commands are described in a sepa rate command description supplied with the PC Interface Kit BE 3 5 Equipping the filter changer I The patented FLUOIII filter system consists of a filter changer for barrier and excitation filters a built in motorized shutter and a filter insert for a filter holder with your choice of filter The filter changer can hold a total of 4 filter sets The filter sets for fluorescence are marked and equipped with a transponder for automatic filter detection see page 17 for details Three filter holders without transponders are included in the scope of delivery of the Leica MZ16 FA These filter holders have two empty openings for the observation beam paths The third opening to the illumination beam path is closed A If you are using fewer than four filter hold ers with fluorescence filters
43. sources Various accessories Video system Leica IC A integrated Photography Digital camera systems for fluorescence Leica DC350 F DFC350 F DC300 F DFC300 F DC300 FX DFC300 FX DC350 FX DFC350 FX DC480 DC500 Leica MPS60 modular photomicrographic system with data back Software For image grabbing archiving processing and analysis Leica Image Manager with Image Overlay and Multifocus modules FW4000 QWin Measuring Measurement graticule for automatic calibration and measurement Leica MZ16 FA Annex 33 Technical data Weight Leica MZ16 FA optics carrier 3 14 kg Lamp housing 2 5 kg Filter set 20 g Manual controls 390 g Planapochromatic objective 1x 21 1 kg Focusing drive coarse fine with column 1 15 kg Microscope carrier 0 495 kg Power pack Input voltage 85 V to 264 V Input frequency 47 Hz to 63 Hz Output voltage 12 VDC Supply connector IEC 320 Low voltage connector Mini DIN 5 pole Operating temperature 10 C to 40 C Relative humidity 85 96 Storage temperature 20 C to 55 C ebq 100 supply unit For indoor use only Supply voltage 90 250 V Frequency 50 60 Hz Power consumption 155 VA max Fuses 2xT2A IEC 127 Ambient temperature 15 35 C Relative humidity maximum 80 up to 30 C Overvoltage category Il Degree of pollution see inst
44. t be opened manually e during the filter change process e ifthe system recognizes a filter holder in the filter changer without a transponder in the beam path e if you remove a filter holder from the filter changer and then turn the filter changer to an empty position The UV shutter opens automatically if a fluores cence filter with transponder is in position If this occurs you can close or open the UV shutter using the Sh button on the manual controls Sh UV shutter open close When work is interrupted you do not have to switch off the high pressure mercury burner to preserve sensitive preparations from burning out or bleaching out Frequently switching the mer cury lamp on and off decreases its life and causes delays as the lamp can only be lit after it has cooled off Instead you can close the UV shutter at the touch of a button and reopen it when you return to your workplace 4 5 6 Double iris diaphragm DI You can use the D to regulate the aperture and enhance the depth of field for plastic specimens On screen display of the aperture with light emit ting diodes Each light emitting diode stands for 20 If all LEDs are lit the double iris diaphragm is completely open 22 4 5 7 Store the current position Set The MZ16 FA offers you the ability to reproduce your multifluorescence experiments exactly cut ting the time needed for this immensely Store the typical working procedures with the desired posi ti
45. tative You will find the address of your nearest local representa tive as well as valuable information about Leica Microsystems products and services from Leica Microsystems on our web site at www leica microsystems com We are gladly at your service Customer service is a big thing with us Not only before the sale but afterwards as well Leica Microsystems Switzerland Ltd Business Unit Stereomicroscopy www stereomicroscopy com Leica MZ16 FA User manual Operating controls Fig 1 Leica MZ16 FA with ErgoTube 1 Display with function keys 2 Motorized filter changer with four filter holders 3 Filter holder for an individually selectable filter 4 ErgoTube with variable viewing angle of 10 50 Additional operating controls see page 38 3 5 Adjustable eyepiece tubes for adjusting the interpupillary distance from 52 76mm 6 Knurled rings to adjust diopters from 5 to 5 7 Adjustable and attachable eyecups 8 Motorized focus system on off switch Leica MZ16 FA User manual Contents Page 1 Safety concept uusuuueuue 11 The user manual 2 00000 5 14 1 Diagrams oec 5 1 1 2 Symbols used 0 0 eee eee 6 1 2 Safety instructions 0005 6 1 3 IGhPSOUrCE seis 5 ee al 9 2 Overview of the instrument 10 3 Assembhly seesseee 3 1 Assembly sequence
46. te illumination beam path for intensive fluorescence illumination coupling to the zoom optics FLUOIII Filter System Horizontally integrated quick changer for 4 filter sets motorized automatic and motorized UV shutter Fluorescence filter transponder Excitation filter and barrier filter on a filter holder with Filter sets GFP GFP Plus GFP Plants UV violet blue green CFP YFP TXR DSR with transponder filter holder without transponder for transmitted light Filter holder without transponder For transmitted light or incident light observation without fluorescence Light source High intensity mercury lamp 50 or 100W lamp housing 106 z chro matically corrected collector focusable centerable lamp mount Safety measures UV protection screen UV barrier filter stray light protection for lamp housing filter holder for transmitted light or empty filter positions automatic UV shutter for filter change and transmitted light Optical data Zoom 16 1 motorized Switchable zoom positions for repetitive tasks 5 individually stored positions Data Zoom range Resolution Numerical aperture Field of view with standard optics objective 1x eyepieces 10x 7 1x 115x 420 Lp mm 0 14 29 6mm 1 8mm Maximum values based on optics combination Magnification 920x Resolution 840 Lp mm Visible structural width 0 6 micron
47. tement of confor mity with EC requirements Health risks A Workplaces with stereomicroscopes both improve and make visual tasks easier but they also place high demands on the human visual apparatus and the muscular holding apparatus of the user Depending on the duration of uninter rupted work asthenopia and musculoskeletal problems may occur For this reason appropriate measures for reduction of the workload must be taken Optimal arrangement of workplace work assignments and work flow changing tasks frequently Thorough training of the personnel giving consideration to ergonomic and organization aspects The ergonomic design and construction of Leica M series stereomicroscopes are intended to reduce the exertion of the user to a minimum A Direct contact with eyepieces is a potential transmission method for bacterial and viral infec tions of the eye The risk can be kept to a minimum by using per sonal eyepieces for each individual or detachable eyecups Leica MZ16 FA Safety concept 1 P 3 Light source safety instructions recautions by the manufacturer UV protection screen in front of the specimen plane prevents the user from looking directly into the UV rays Automatic shutter locking during rotation of the filter changer observation without fluores cence and removal of the filter holders pre vents UV radiation from reaching the eyes Filter holders without transponders in the free
48. xample 50 0 gt Initialize the calibration using the CAL button on the Leica MZ16 FA Leica MZ16 FA Use Selecting a calibration mode gt Use the Zzoom button on the manual controls to select a calibration mode magnification C magn or reference distance C dist in the example itis C magn gt Confirm and display your selection using the SEL button beep Depending on the selected calibration mode continue with the section Calibrating CM or Calibrating CD Calibrating magnification CM Display after confirming the selection CM 50 3 in the example CM process start Look into the eyepieces In the example we are working with 10x eyepieces Focus the stage micrometer Inthe example the diameter of the small circle of 7mm at zoom position 50 with 10x eye pieces corresponds to a distance of 1 40mm on the specimen micrometer 7 5 With 16x eyepieces the distance corresponds to 2 24mm Adjust the magnification using the ZWwheel on the manual controls exactly so that the diameter of the small circle inside line matches the dis tance of 1 40 on the stage micrometer Confirm and display the calibration press the CAL button twice beeps e Inthe display appears in the example A 50 i e A mark of zoom position at which calibration was performed Leica MZ16 FA Use Calibrating the reference distance CD Initialize the calibration and select the calibration mode see p
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