64 Channel Recording System
Contents
1. Wireless Headstage Introduction The Triangle BioSystems International TBSI W64 wireless recording headstage system allows researchers to continuously and simultaneously monitor up to 63 single ended biopotentials as single units EEG and ECOG Differential signals such as an EMG recording can easily be accommodated in software No longer do experiments have to be constrained by tethering a test subject The complete system is comprised of a wireless headstage transmitter with integrated battery RF signal receiver baseband demodulator power supply and all required cables With an effective range of 4 meters this system provides a wireless connection between the implanted electrodes and the data recording system TBSIs custom ASIC technology and proprietary radio design provide high data rates 50 kHz channel in a wireless headstage that is both small and light weight 5 0 g This design also incorporates preamplifier circuitry to create an extremely compact and powerful transmitter Features e Wireless operations across 4 meters e 63 single ended recording channels Headst e Headstage transmitter weight 4 5 grams edtatase e Default Total System voltage gain of 800 other gain offerings available e Rechargeable battery with 3 5 4 2 hours of battery life e Bandpass filtering per channel at 8 Hz to 7 kHz typical e 50 kHz per channel sampling rate e Operates in unlicensed radio band below FCC limits e2. Charge Plug a Turn off the headstage transmitter using the magnetic wand blue light will turn off as indication b Connect the battery charging adapter to a wall socket LED Charge Indicator Headstage with c The white charging plug is keyed and aci assar mates with the white socket on the side of the headstage Connect the charging cable to the headstage making sure that the connection is sound d The indicator light on the charging unit is red while charging and green when the connected battery is halfway recharged A dead battery will become fully charged within approximately one and a half hours after the light has turned green 2 Correctly position the receiver near the animals cage It is critical for transmission performance to correctly position the receiver next to or inside the animals cage First screw the antennas onto both antenna clamps gold input SMA connectors located on the side of each clamp Attach the SMA connector of the antenna cable to the mating SMA connector on the receiver box For best results position the antennae at 45 degree angles with respect to the cage area and aim the them towards center of the cage Version 3 0 13 1 2014 W64 System User s Manual 3 Connect power supply to receiver Plug the DC end of the power supply cable into the back of receiver and the other end into an AC outlet The recording system is powered by an AC line adapter transformer regulato
3. M an o TT si wm w cd co 5 y A Ww Db ii in n TT TF m mm m E v Y RA a 92 92 2 297 92 92 2 92 ON QQ TT 7 TT TT PT 7 7 Ping Pinfzo Version 3 0 10 1 2014 W64 System User s Manual Version 3 0 Red Pin 1 36 18 19 37 Description GND Channel 2 Output Channel 4 Output Channel 6 Output Channel 8 Output Channel 10 Output Channel 12 Output Channel 14 Output Channel 16 Output Channel 18 Output Channel 20 Output Channel 22 Output Channel 24 Output Channel 26 Output Channel 28 Output Channel 30 Output Channel 31 Output Channel 1 Output Channel 3 Output Channel 5 Output Channel 7 Output Channel 9 Output Channel 11 Output Channel 13 Output Channel 15 Output Channel 17 Output Channel 19 Output Channel 21 Output Channel 23 Output Channel 25 Output Channel 27 Output Channel 29 Output Channel 32 Output No Connection ls Blue Pin 1 36 34 18 19 37 TBSI Description GND Channel 34 Output Channel 36 Output Channel 38 Output Channel 40 Output Channel 42 Output Channel 44 Output Channel 46 Output Channel 48 Output Channel 50 Output Channel 52 Output Channel 54 Output Channel 56 Output Channel 58 Output Channel 60 Output Channel 62 Output Channel 63 Output Channel 33 Output Channel 35 Output Channel 37 Output Channel 39 Output Channel 41 Output Channel 43 Output Channel 45 Output Channel 47 Output Channel 49 Output Channel 51 Output Channel 53 O
4. the RF receiver box Also verify that the yellow Signal Lock light is illuminated which confirms that the receiver is receiving the signal transmitted from the headstage Problem Visible neural signal is missing information Suggestion Keep the animal within the 4 meter range of the receiver If you exceed this range the radio signal from the headstage will not be strong enough to maintain reliable signal monitoring of the animal Also be sure to keep the area under the RF receiver unit s antennas free from metallic objects which will reduce signal range and introduce noise Problem Not all channels are visible on the neural signal Suggestion Make sure the headstage connection to the animal is secure Version 3 0 19 1 2014 W64 System User s Manual TBSI Application Notes 1 The wireless headstage is a low power device it is critical that the RF receiver be carefully located for the system to operate DO NOT place the receiver on the outside of a metal wire cage Try to minimize the distance between the receiver and the wireless headstage Please refer to the section in this document entitled System Setup and Testing for orientation suggestions 2 If you do not intend to record from all of the available signal channels you must ground any unused channels at the electrode connector or EIB interface Failure to do so can create artifact noise from the floating channels on the other signal channels 3 H
5. Covered or dipped headstage options Receiver with Dual Antennae Version 3 0 3 1 2014 W64 System User s Manual System Configuration The receiver antennas must be clipped to a surface within 4 meters from the animal at all times to achieve optimal signal quality They should be positioned above the animal s cage Be careful not to obstruct the line of site path between the animal and the receiver antenna with any material except for glass or plastic See the section entitled System Setup and Testing for more details on proper system assembly and positioning TBSI NeuroWare Software Analog Receiver with ADC Board Wireless Headstage Standard TBSI Wireless Recording Setup System Block Diagram The wireless neural headstage system consists of a wireless transmitter headstage an RF receiver and baseband demodulation subsystems as shown below Headstage Transmitter Time mb Division Multiplexer Pre amplifiers High BW Amplifier Electrode i Pass nputs Filters Transmit Antenna RF Filter amp 60dB RF FM Demodulator To Neural Seen E DAQ PLL and Cik Analog Recovery ERE Are Ae ee De multiplexer i GT ee S Ev System EE NOTE These products are not intended for human use Version 3 0 4 1 2014 W64 System User s Manual Wireless System Parts List Wireless Sy
6. Intermediate gain 10 20 dB e Input referred noise typical 4 uV RMS e Input voltage range 0 5 V e Analog channel bandwidth 20 kHz e DC offset lt 100 uVdc e Phase delay typical 30 usec at 10 kHz e Signal lock indicator LED on front panel RF Receiver Mechanical Overview 1 On Off Switch 2 Signal Lock LED Power On LED 3 Antenna SMA Connectors 2 4 Analog Output Connector DB37 5 GND Connector Option 6 Receiver Power Supply Connector DC6V O 8A 9 25 7 Receiver Antenna 2 8 Antenna Extension Cable Clamp 2 1 75 Version 3 0 9 1 2014 W64 System User s Manual RF Receiver Signal Interface Options The W32 RF Receiver Base Station is available in many different system configurations to accommodate a wide range of data acquisition system interface requirements The RF receiver signal is demodulated by a custom digital signal processing system that incorporates a high speed analog to digital converter for demodulating the receiver signal The demodulated signal is then conditioned via the DSP and converted to an analog signal The analog signal can be directly output to one or two DB 37 connectors input to an integrated Analog to Digital Converter ADC or both Receiver Signals Analog Output Arey CA Power Pq ly Lo iy F 22195 Y QQ Qu Ch32 Ch30 Ch28 Ch26 Ch24 Ch22 Ch20 Ch18 Ch16 Chi4 Chi Chio h h h h iI OG LO Finfi Pinfzo
7. Triangle BioSystems TBSI W64 System Manual Version 3 0 Triangle BioSystems International 2224 Page Rd Suite 108 Durham NC 27703 Phone 919 361 2663 Fax 919 544 3061 www trianglebiosystems com W64 System User s Manual Contents 64 CHANNEL WIRELESS NEURAL HEADSTAGE SYSTEM 3 io Ro 3 Bac Us ARO A Dn o o re re er 3 NNN UN 4 ysteMBIOCKDAPffilia ar 4 Wireless System PAS 5 Headstage Transmitter NNN 6 Headstage Mechanical Overview ve 7 MENE 8 RF Receiver TON g RF Receiver Mechanical Overview r rrrrnnrnnnnnnrrnnvnnnnnrrnnnvnnnnnsennnvnnnnnsrnnnnnnnnnsennnnnnnnnsnnnnnnnnnnsennsnnnnnssennsnnne 9 RF Receiver Signal Interface OPTIONS eesssessssessessreresrrrssrresrrrrsrrrnsrtrssrerssrresrtresrtessreessteesrtrssreeserersreresns 10 Receiver Signals Analog OUtput ie 10 Receiver Signals Digital Interface usina iia 12 System etup ana TOSTE EE tua pretura 13 internal and External Batteries 16 Mouse and NENNE 17 Me NNN 17 LED SACS Fa OO og 18 HeadgstaseRemovealtTooi alli 18 Headstage Electrode Connectors Alignment Tool ccccescccsecccescccncccenecceneceeececenseeeueeteneceeeceeeneens 18 Wireless Power TENNENE 18 A RD RR ERR EE 18 PN 19 TOUDE NOONE rea 19 APpucatiOn NOTE nai ai i 20 NN 20 Version 3 0 2 1 2014 W64 System User s Manual 7 TBSI 64 CHANNEL WIRELESS NEURAL HEADSTAGE SYSTEM TBSI NeuroWare Software Analog Receiver with ADC Board
8. eadstage Rubber Band Dental Acrylate Metal Hooks ui Lo dg Animal Skull Contact Us Triangle BioSystems International 2224 Page Rd Suite 108 Durham NC 27703 Phone 919 361 2663 e Fax 919 544 3061 www trianglebiosystems com Need more help You can reach TBSI customer support by phone at the number listed above M F Eastern US Time or email us at support Qtrianglebiosystems com If you need replacement parts or accessories for your system or to learn the latest about available TBSI products visit our website call or email us at sales trianglebiosystems com CAUTION This product is not registered with the FDA and is not for clinical use on human or veterinary patients It is intended for research use only CAUTION FOR RESEARCH USE ONLY NOT FOR CLINICAL USE ON PATIENTS Version 3 0 20 1 2014
9. herefore you can expect the analog output values to be about 800 Vp p of the signal from the function generator Version 3 0 14 1 2014 W64 System User s Manual 7 Test headstage signal transmission with the W32 Signal Input Test Cable a Line up the two guide pins on the headstage with the guide pin holes on the test cable Make sure that the labels on the connectors face the same direction b W32 Signal Input Test Cable 200 0021 10 supplied with receiver c Signal leads connect to positive side of function generator d GND Leads connected to signal Ground side of function generator Input a 1 kHz sine wave signal at maximum 4 mVp p amplitude The function generator signal output voltage cannot exceed 4 mVp p otherwise the headstage input voltage range will saturate e Once the test cable has been attached use the magnetic wand to turn on the headstage Briefly hold the black tip of the wand next to the top of the headstage until you see the blue power on LED The headstage will transmit data continuously while this LED is on 8 Inductive Power Headstage and Charging TOP VIEW pr 1 Red battery is IP Tranmit Coil MN enabled 2 Green battery is charging Power Supply Se AR G Blue transmitter is enabled If the wireless power charging accessory is used please follow the steps below for charging the headstage a Plug in the Inductive charger power supply Turn the t
10. nel 63 20 Channel 62 3 Channel 61 21 Channel 60 4 Channel 59 22 Channel 58 5 Channel 57 23 Channel 56 6 Channel 55 24 Channel 54 7 Channel 53 25 Channel 52 8 Channel 51 26 Channel 50 9 Channel 49 27 Channel 48 10 Channel 47 28 Channel 46 11 Channel 45 29 Channel 44 12 Channel 43 30 Channel 42 13 Channel 41 31 Channel 40 Fin 18 Pin 36 Pin 54 Pin 72 14 Channel 39 32 Channel 38 15 Channel 37 33 Channel 36 16 Channel 35 34 Channel 34 17 No Connection 35 Channel 33 18 ACgnd 36 ACgnd Pin Pin Connection Pin Pin Connection 37 ACgnd 55 ACgnd 38 Channel 31 56 Channel 32 39 Channel 29 57 Channel 30 40 Channel 27 58 Channel 28 41 Channel 25 59 Channel 26 42 Channel 23 60 Channel 24 43 Channel 21 61 Channel 22 44 Channel 19 62 Channel 20 45 Channel 17 63 Channel 18 46 Channel 15 64 Channel 16 47 Channel 13 65 Channel 14 48 Channel 11 66 Channel 12 49 Channel 9 67 Channel 10 50 Channel 7 68 Channel 8 51 Channel 5 69 Channel 6 52 Channel 3 70 Channel 4 53 Channel 1 71 Channel 2 54 ACgnd 72 ACgnd Version 3 0 8 1 2014 W64 System User s Manual TBSI RF Receiver Specifications e Three available transmission frequencies o F1 3 05 GHz center transmit frequency with 100 MHz FM bandwidth o F2 3 375 GHz center transmit frequency with 100 MHz FM bandwidth o F3 2 725 GHz center transmit frequency with 100 MHz FM bandwidth e 4 0 M maximum range between headstage transmitter and receiver antennae e Front end gain 60 dB e
11. on power headstage HPT High power transmission headstage So moss 100 0001 04 International AC Power Adapters are External Battery Charger 4 7 V External Battery and Headstage included each systems A complete set 2 prong male connector Connector of adapters can also be purchased separately Part AC5 Version 3 0 16 1 2014 W64 System User s Manual Mouse and Rat Saddle Harness Saddle harnesses can be used for external battery options Di Mouse Saddle Harness Rat Saddle Harness Rat Saddle Harness with Mounted 004 0001 10 004 0001 20 500 mAh External Battery Headstage Accessory Options Three biosensor options are available as add ons for any wireless headstage These accessories replace channels typically used for neural recording on the headstage Installation of any of these features increases the headstage length by 0 4 inches its height by 0 2 inches and its weight by 0 4 grams The features can be installed individually or together in any combination See the TBSI Biosensors Brochure for more information Due to the fact that sensor options require 1 or 3 data channels each these may not be practical for use with the W16 headstage consider a W16 or higher headstage Accelerometer This add on will monitor x y and z acceleration vectors and output the information via three analog signal channels The animal head position and velocity can later be calculated with software such as Matlab Ul
12. r This power unit connects to a 100 240 VAC at 47 63 Hz and is rated at 6 VDC at 2 5 A power source and is UL approved Use a suitable International adapter to mate the standard US plug to your AC outlet 4 Connect signal cable to receiver analog output DB37 connector If your system was configured for analog system output connect a suitable analog output cable to the DB37 receiver connector Five signal wires and a ground wire can be added to the DB37 mating connector to check for signal output The lengths of the wires are not critical The analog output channel positions are described in the section entitled Receiver Signal Interface Analog Output If your receiver has an integrated DAQ plug the USB cord into the receiver and PC and follow the instructions provided in the Neuroware Manual or your data acquisition software for signal acquisition and viewing Plug and screw connector into back of receiver box Wires to recording ADC System 5 Turn on Receiver Flip the on off button of the receiver to control power to the receiver 6 Signal Lock LED and measure analog output signals Once the receiver and headstage see 47 below have both been switched on the Signal Lock LED on the receiver front panel should light up After confirming the signal lock integrity you can view the analog signals output from the DB37 connector on the back of the receiver with an oscilloscope Please note the default system gain is 800 t
13. ransmitter on using the power switch on the face of the Inductive power base station b Turn on the headstage charge coil using the RED end of the magnet wand On will be indicated by a RED LED c With the RED LED lit place the headstage in the Inductive transmit field A GREEN LED will indicate that the battery is being charged Charge the headstage for 2 hours to fully charge the headstage battery d Use the BLUE end of the magnet wand to turn on the TBSI headstage for recordings The headstage radio ON is indicated by a BLUE LED Version 3 0 15 1 2014 W64 System User s Manual Internal and External Batteries The TBSI W16 recording headstage is typically configured with a 60 mAh internal battery The W32 headstage can also be built with an internal 75 mAh internal battery for increased recording capacity Alternatively the headstage can be configured for use with an external battery Several different external batteries are available and are listed in the table below For rodent applications we offer both a mouse and a rat saddle harness with Velcro pad See the section entitled Mouse and Rat Saddle Harness below Removing the internal battery will reduce the weight and size of the headstage by an amount comparable to the battery specifications as listed in the following table TBSI W Series External Batteries wet ose ise ass ass wet sag ag s g use 1 Hours of Operation STD Standard transmissi
14. stem Parts List Item Part Number Various Various 110 0001 00 300 0002 10 100 0000 10 100 00001 00 200 0021 40 300 0000 00 202 0001 10 200 0011 00 O Version 3 0 5 1 2014 W64 System User s Manual Headstage Transmitter Specifications PARAMETER MAX UNITS NOTES Current Draw Battery Analog Input Specs 790 for 1 Hz 10 Hz frequency 3 316 uVp p Inputreferrednoise 0 835 arms for 10 Hz 100 Hz frequency 3 34 uVp p Input referred noise 195 uvrms for 100 Hz 1 kHz frequency 7 8 Vp p 50 Sampling Channel kHz At headstage max 30 kHz sampling rate channel at ADC Mechanical Specs 11 Length 250 mm Edge to Edge including connectors Width 11205 mm Edgeto Edge Height mm EdgetoEdge gt Weight 150 8 With connector and dipped package Input connector 1 8PinOmnetics 025 Radio Specs F1 F2 F3 3 05 Transmit antenna 3 05 3 375 2 725 GHz Tuned chip antenna with circular diversity Transmit range 40 40 40 MWith connector and dipped package Version 3 0 6 1 2014 W64 System User s Manual TBSI Headstage Mechanical Overview Top View Bottom View 20 5 mm 25 0 mm Complete headstage weight is 5 0 grams Version 3 0 7 1 2014 W64 System User s Manual Wireless Headstage Pinout Pini Fin19 Pin37 Pin 55 Pin Pin Connection Pin Pin Connection 1 ACgnd 19 ACgnd 2 Chan
15. trasonic Microphone This single ultrasonic microphone enables recording of audio frequencies between 1 kHz and 25 kHz W5 and W32 Headstages or 1 40 kHz W16 and W64 Headstages and uses one recording channel Temperature Sensor A Thermistor based temperature sensor option is available Many sensor sizes are available including very small ones that can be implanted within or near the brain The sensor must be attached to a mating connector and externalized to the head of the animal much like traditional recording electrodes Sensors are customized to your particular research needs Ultrasonic Accelerometer i a fe Microphone R Thermistor Connector Version 3 0 17 1 2014 W64 System User s Manual 7 TBSI LED Headstage Options Red blue or green permanently affixed LEDs or socket mounted LEDs are available for the W32 headstage The LEDs are placed facing upward on top of the headstage and are suitable for use with most video tracking software applications The LEDs will turn on when the headstage is turned on Installation of LEDs on the headstage may reduce battery life by as much as 15 This tool is used to remove a headstage from the rat or mouse s head quickly and efficiently its part number is 300 0006 10 Headsta Electrode Connectors Ali nment Tool 2xA9409 Female 36 pin 4 post 25mil Omnetics Connector Part Number 010 0059 12 Wireless Power Headstage Option This unique wireless power s
16. utput Channel 55 Output Channel 57 Output Channel 59 Output Channel 61 Output No Connection 1 2014 W64 System User s Manual Receiver Signals Digital Interface TBSI offers an internal DAQ board option and NeuroWare software for the 5ch 16ch 32ch and 64ch receivers which can be used for signal data acquisition and analysis Digital output and event input information is sent to the PC via a USB connection The total system gain for the digital receiver is 800 The integrated data acquisition card can also be read using DAQ toolkits from MatLab and Labview Ener Log Opablap E me Spin Chd Chl Spoke ChUT Chd2 Muti Charmai timer Neuro Ware O teran ST EE Record Duta Spike Display ietimi Fillers Feference Graph Sele Aulo Trigger eli r Ader Souk Yih Il Tagger 3 TEO DI To ER Pra Spike DS RIS n Data Select Cuplay Hide Miradas idik dra Timactame ix gd escada ne y CJ Frio Wiri Sos p Chat E 100 E Di Tuesholi care Apply Conent Threshpli bo Al Channels Version 3 0 12 1 2014 W64 System User s Manual System Setup and Testin The steps below should be followed when setting up your W32 system to optimize its performance 1 Charging the Headstage Lithium Ion Battery The wireless headstage includes an integrated rechargeable battery which should be recharged when not in use Recharging is accomplished by the following procedure
17. ystem is designed to keep a wireless headstage internal battery perpetually charged thereby enabling indefinitely and uninterrupted device operation There are 4 major components including the power amplifier transmitter transmitter coil around the animals cage power supply and headstage pickup coil with harvester Since the transmitter coil is carefully tuned and sized for the animal cage dimensions it is important to note that the wireless power transmit coil must be placed carefully on a bench top or cage rack without nearby metal and at least 15 inches from other TBSI wireless powering systems Electrodes Many electrode manufactures offer electrode arrays compatible with TBSI wireless headstages Cambridge Neurotech offers an extensive line of silicone probes suitable for chronic signal recordings from our wireless headstages Compatible electrode arrays are also available from Microprobes Inc NeuroNexus and others electrode providers Version 3 0 18 1 2014 W64 System User s Manual TBSI Gain and Phase Response Please refer to the section of this document entitled Headstage Transmitter Specifications for numerical data on bandwidth and input referred noise LAB i A F fedd Troubleshooting Problem No neural signals are visible on any of the analog outputs at the DB37 connector Suggestion Verify the AC power connection is in place and the green Power LED is illuminated on the front of
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