Beta-Pette Single Channel User Manual 2001
Contents
1. Volume change AV Range Preset Permissible for full tum of the Model of the pipette volume volumes ka ji volumes ul Mi ull calibration key ul 24 increments B2 0 1 2 0 2 0 176 0 224 0 06 BAO 0 5 10 0 5 0 48 0 52 0 33 B 20 2 20 2 1 92 2 08 0 63 B100 10 100 10 9 84 10 16 2 50 B 200 20 200 20 19 76 20 24 6 30 B 1000 100 1000 100 99 1 100 9 25 00 B 5000 1000 5000 994 1006 e Perform 5 aspirations weigh each one and calculate the average value of the aspirations e Calculate average aspirated volume in ul mul tiplying the average aspiration amount mg by the distilled water density coefficient ul mg which depends on temperature and pressure according to the following table Pressure hPa Temperature C 960 1013 1067 20 1 0028 1 0029 1 0029 21 1 0031 1 0032 1 0032 22 1 0032 1 0033 1 0033 23 1 0035 1 0035 1 0036 24 1 0037 1 0038 1 0038 25 1 0039 1 0040 1 0041 If the average aspirated volume exceeds the permissible value the following should be done e Remove the pipetting pushbutton Fig 4A e Holding the volume setting knob to protect it against rotation insert the calibration key into the cuts of the calibration screw Fig 4B e Turn the key clockwise to reduce the aspirat ed volume or counter clockwise to increase the volume Fig 4C One full turn of the cali bration key2. Beta Pette Continental Lab Product 5648 Copley Drive San Diego CA 92111 Phone 1 800 456 7741 Beta Pette Beta Pette 4A 4C Fa K 4B st gt 4 1771 TOD NH Beta Pette 1 INTRODUCTION 2 SETTING THE VOLUME 3 OPERATION 4 PRE RINSING 5 DENSE AND VISCOUS LIQUIDS 6 CLP PIPETTE TIPS 7 RECOMMENDATIONS 8 RECALIBRATION 9 TROUBLESHOOTING 10 PIPETTE KIT AND ACCESSORIES 11 SPARE PARTS 1 INTRODUCTION The Beta Pette is a volumetric instrument designed to measure and transfer liquids pre cisely and safely It can measure and transfer volumes from 0 1 pl to 5000 ul depending on the model The Beta Pette has a digital volumeter which displays the volume The volume is adjusted by turning the black knurle
3. of your pipette request document DIN 12650 The pipette design enables the user to perform the recalibration process according to the rules presented in section 8 2 SETTING THE VOLUME The volumeter consists of three numbers and is read from top to bottom The three numbers indicate the volume selected and are colored black and red For the 8 2 8 10 8 20 B 100 and 8 200 pipettes the numbers in black represent micro liters and the numbers in red represent tenths and hundredths of microliters AN EXAMPLE FOR EACH OF THESE PIPETTES IS GIVEN BELOW B 2 B 10 B 20 100 200 1 0 1 0 1 red 7 2 7 2 red s red 5 red 5 5 1 25 ul 7 5ul 12 50 u 75 ul 125 ul For the 8 1000 and 8 5000 pipettes the num bers in red represent milliliters and the numbers in black represent microliters AN EXAMPLE FOR EACH OF THESE PIPETTES IS GIVEN BELOW 8 1000 8 500 o red l red 7 2 5 5 0 75 m 25m The volume of the pipette is set by turning the black knurled adjustment ring Fig 1B To obtain maximum accuracy when changing the volumeter setting follow the recommendations below e When decreasing the volume setting turn the adjustment ring slowly to reach the required setting making sure not to overshoot the mark e When increasing the volume setting turn the adjustment ring until you are 1 3 of a turn above the required setting Turn the adjust ment ring slowly
4. All the spare parts indicated in Fig 3 4 that is A Pushbutton G Piston assembly B Calibration knob H Spacer C Shaft I O ring D Ejector J Seal F Shaft nut K Calibration key can be ordered from Continental Laboratory Products Please specify pipette type and part name when ordering Warning The replacement of the plunger requires conducting of calibration proce dure according to section 8 B 04 2001
5. or down to take account of the remaining error When dispensing dense or viscous liquids wait one or two seconds longer at the first stop before expelling the residual liquid 6 CLP PIPETTE TIPS Pipette tips from Continental Laboratory Products are made from high quality polypropy lene and most feature our exclusive S low retention polymer technology No other tips can 6 Beta Pette match their accuracv and precision Call and ask for our complete technical report CLP tips also feature our esp packaging svs tem for quick trav reloading and reduced waste and storage space Look for esp10 esp200 esp1000 The accuracy and precision of the Beta Pette are only quaranteed when CLP tips are used Inferior quality tips can seriously reduce the per formance of these precision instruments 10 pl Capacity Tips For sample volumes 0 1 to 10 ul Cat Series 2040 2140 2340 2047 esp10 Bt10 Bt10XL 8 Bt10E 200 ul Capacity Tips For sample volumes 2 to 200 ul Cat Series 2000 2100 amp 2007 esp200 2030 2180 2150 Bt20 Bt100 amp Bt200 1000 pl Capacity Tips For sample volumes 100 to 1000 pl Cat 2160 2110 2067 esp1000 Bt1000 5 ml Capacity Tips For sample volumes 1 to 5 ml Cat 2250 7 RECOMMENDATIONS The recommendations below will ensure maximum accuracy and precision from your Beta Pette e Make sure to operate the Geta Pette slowly and smoothly e The depth of immersion in the sa
6. changes the pipette aspiration vol ume by the amount given in the table e Take out the key and fix the pipetting push button Fig 4D Determine the average aspirated volume The average volume should be within the permissi ble range given in the table If the volume exceeds the values stated the recalibration pro cedure should be repeated In case of pipetting the liquids with physical properties considerably different from those of water follow the rules given in section 5 9 TROUBLESHOOTING If there is a leak or the pipette is not accurate check for the following e The connecting nut is loose Fig 3B Tighten the connecting nut Beta Pette e The shaft is cracked or scored Remove the tip ejector and inspect the shaft For the 8 2 8 10 and 8 20 models if the shaft is damaged the piston may also be damaged Replace the damaged parts see Section 11 When reassembling the unit the nut should be hand tightened e Chemical damage to the piston and seals Replace the piston and seals See Section 11 Wash the inside of the shaft using distilled water e Improper reassembly Disassemble the pipette and reassemble the pipette correctly Fig 3 If there is liquid in the shaft clean the pipette as follows e Remove the tip ejector Unscrew the shaft nut and rinse the shaft piston seal and O ring with distilled water Dry these parts and reassemble the pipette Note If necessary the shaft sha
7. d adjustment ring Fig 1B and is continuously adjustable within the volume range for the pipette The maximum volume for the pipette is shown on the pushbutton and corresponds to the model number Fig 1A The volume range from 0 1 ul to 5000 ul is cove red by seven pipette models 1 Model Volume range ul B2 01 2 B 0 0 5 10 B 20 2 20 B 100 10 100 8 200 20 200 8 1001 100 1000 B 5000 1000 5000 B2 B10 Measurement and transfer of micro volumes DNA sequencing and enzyme assay applications Measurement and transfer of general aqueous B 20 8 100 8 200 8 1000 a solution acids and bases B 5000 Measurement and transfer of large volumes The Beta Pette uses disposable polypropylene tips Fig 1E The disposable tips ensure maxi mum safety for the user and no cross contami nation between samples To protect the user from contamination by the tips the Beta Pette is equipped with a built in tip ejector Fig 1D The construction of the ejector enables the user to adjust the length The adjustable tip ejector accommodates most of tips available on the market When using narrow tubes it may be necessary to remove the tip ejector It is simply removed by pulling down Adjusting Tip Ejector Length The H spacers included in the box are used to lengthen the tip ejector by 1 and 2 mm The spacers are differentiated by the number of ridges on the body see fig
8. ft nut and the tip ejector can be autoclaved for 20 minu tes at 121 C and at 1 bar pressure The seal and the O ring should not be autoclaved The outside of the Geta Pette can be cleaned using a cloth dampened with isopropanol If there is an air bubble when the sample is aspirated e Eject the sample into its original vessel e Check that the tip is properly immersed in the same liquid e Pipette the sample more slowly If the bubble appears for the second time replace the tip If a problem continues after carrying out the above steps please call Continental Laboratory Products for assistance Before returning a pipette to CLP please ensure that the instrument is completely free of any chemical microbial or radioactive contami nation 11 10 PIPETTE KIT AND ACCESSORIES Pipette kit The pipettes are delivered in the kits including Pipette e Instruction manual e Calibration key e Ejector regulation spacers e Identification labels e Pipette shelf clip The pipette shelf clip diagram is shown in Fig 5 Accessories Pipettor Volume Range Tip Choices B 2 and B 10 0 1 10 ul 2040 2340 2047 B 20 2 20 ul 2000 2100 20077 B 100 10 100 ul 2000 2100 20077 B 200 20 200 ul 2000 2100 20077 B 1000 100 1000 pl 2162 2167 8 5000 1000 5000 ul 2250 Designates esp environmentally sensitive packaging SEO B 2 to B 5000 9577 Carousel Stand 10 SPARE PARTS
9. ifferent liquid is being sampled or if the volumeter setting is changed 4 PRE RINSING When pipetting liquids which have a viscosity and density different than water for example organic solvents a film of liquid is formed on the inside wall of the pipette tip This film can create an error Since the film remains relatively constant in successive pipetting operations with the same tip this error can be avoided by form ing the film before transferring the first sample This is done by aspirating a sample and dis pensing it back into the same vessel Since the film is already formed all of the following sam ples will have better accuracy and repeatability This pre rinsing operation should be repeated when the volume to be aspirated is changed or when a new tip is used 5 DENSE AND VISCOUS LIQUIDS For moderately dense or viscous liquids com pensation is possible by setting the volumeter higher than the required value For liquids which are less dense than water compensation is possible by setting the vol umeter lower than the required value Example to transfer 10 ul of serum using Beta Pette model 8 20 Set the volumeter to 10 ul Aspirate a volume of liquid and measure it gravimetrically If the cor responding volume is measured as 9 5 ul the error is 0 5 ul Increase the volumeter setting by 0 5 ul to 10 5 ul and repeat the measurement If the second measurement is not accurate enough adjust the volumeter up
10. mple liquid should be the minimum necessary and should remain constant during aspiration The eta Pette should be held in a vertical position e Change the tip when volume setting is changed or when a different liquid is to be aspirated e Change the tip if a droplet remains on the end of the tip from the previous pipetting opera tion e Each new tip should be pre rinsed with the liq uid to be pipetted except when using S tips e Liquid should never enter the Beta Pette shaft To prevent this Press and release the pushbutton slowly and smoothly Never turn the pipette upside down Never lay the pipette on its side when there is liquid in the tip e Never force the volumeter beyond its recom mended limits e When pipetting liquids with temperatures dif ferent from the ambient temperature it is rec ommended to pre rinse the tip several times before use e Do not pipette liquids with temperatures above 70 C e When pipetting acids or corrosive solutions which emit vapors it is recommended to dis assemble the shaft and to rinse the piston and seal with distilled water after finishing the pipetting operation 8 RECALIBRATION The pipette is calibrated by gravimetric method using distilled water at the temperature 20 1 C according to DIN 12650 If during pipette operation you find that the accuracy error the difference between the real aspirated volume and the preset volume exceeds the permi
11. ssible value given in the table in section 1 the pipette recalibration procedure should be carried out Before starting the recalibration it is necessary to check whether the following requirements have been fulfilled during error determination e the ambient temperature and the temperature of the pipette tips and water was identical e the density of the liquid used was close to that of distilled water ethe balance with appropriate sensitivity has been used Beta Pette Volume checked ul Balance sensitivity mg 0 1 10 lt 0 001 10 100 lt 0 01 gt 100 lt 0 1 e mg l conversion factor has been taken into account ethe requirements given in sections 3 and 7 have been fulfilled If the above conditions are satisfied and the ac curacy error for selected volume given in section 1 exceeds the permissible value the pipette recalibration procedure should be carried out The recalibration can be performed within one full turn of the key to the right or to the left only Recalibration conditions e Ambient temperature and the temperature of the pipette tips and liquid should be within the range 20 25 C and stabilized during weighing within 0 5 C e Measurements should be conducted using distilled water e Balance sensitivity should be suitable for the volume to be controlled Recalibration procedure e Set the dose volume depending on the pipette volume according to the following table
12. to decrease the volume to reach the required setting making sure not to overshoot the mark Beta Pette 3 OPERATION Place a tip on the shaft of the pipette See Section 6 for the appropriate tip Press the tip on firmly using a slight twisting motion to ensure a positive airtight seal Note Never handle a liquid with a Beta Pette which has not been fitted with a tip Aspiration Press the pushbutton to the first positive stop Fig 2A Holding the pipette vertically immerse the tip into the sample liquid The depth to which the tip is immersed in the sample liquid depends on the model B 2 and 8 10 B 20 and 8 100 8 200 and 8 1000 8 5000 Release the pushbutton slowly and smoothly to aspirate the sample Fig 2B Wait one second and then withdraw the tip from the liquid Wipe any droplets away from the outside of the tip using a medical wipe Avoid touching the orifice of the tip Dispensing e Place the end of the tip against the inside wall of the vessel at an angle of 10 to 40 degrees e Press the pushbutton smoothly to the first stop Fig 2C Wait one second e Press the pushbutton to the second stop to expel any remaining liquid Fig 2D e Keeping the pushbutton pressed to the end remove the pipette by drawing the tip along the inside surface of the vessel Release the pushbutton Fig 2E e Eject the tip by pressing the tip ejector button Fig 2F It is only necessary to change the tip if a d
13. ure 1 L The stan dard spacer HO has no ridges Note That 8 5000 does not have a tip ejector The eta Pette is a high quality instrument which offers excellent accuracy and precision The figures for accuracy and precision given in the following table were obtained using tips from Continental Laboratory Products and can only be guaranteed when these tips are used Beta Pette SPECIFICATIONS Volume Accuracv Precision Model lull 9 9 0 2 12 0 lt 6 0 B2 1 0 2 7 lt 1 3 Max 2 0 15 lt 0 7 Min 0 5 4 0 lt 4 0 BAO 5 0 1 0 lt 0 8 Max 10 0 0 5 lt 0 4 Min 2 4 0 lt 3 0 B 20 10 1 0 lt 0 7 Max 20 0 8 lt 0 4 Min 10 1 6 lt 0 8 B 100 50 1 0 lt 0 4 Max 100 0 8 lt 0 3 Min 20 1 2 lt 0 6 B 200 100 1 0 lt 0 3 Max 200 0 6 lt 0 3 Min 100 0 9 lt 0 45 B 1000 500 0 7 lt 0 25 Max 1000 0 6 lt 0 25 Min 1000 0 6 lt 0 3 B 5000 2500 0 6 lt 0 3 Max 5000 0 5 lt 0 2 These specifications are obtained in forward mode using a gravimetric method with the tem perature of the distilled water tips and all other conditions stabilized between 19 C and 21 C Number of measurements minimum 10 The values given include all components of error due to both normal handwarming and the changing of the tip Performance tests These performance tests are derived from those prescribed by the Deutches Institut fur Normung e V To verify the performance
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