NeuroPort® - Blackrock Microsystems
Contents
1. Wave Bass Sue Ven Sno n B feo Se aod M Cd Visi fanden Mamun Smitaneous Channels iia Xcharnela fre at the same time their Haba pn uu om tmd Figure 33 Manual PCA Spike Sorting Properties Method Choose between the automatic Histogram Peak Count and Manual PCA methods Method Histogram Peak Count Feature space sample points are projected onto an axis and a histogram is constructed Clusters are then estimated by determining peaks and valleys and are sorted into units Valley Percentage The first peak from the origin is detected and marked To find a valley the valley height needs to be less than the first peak s height x the valley percentage value valley height lt first peak height x valley percentage Close Peak Percentage For a second peak to be found the second peak height needs to be greater than the height of the valley the height of the 3 points around the peak the close peak percentage second peak height gt valley height 3 point mean around valley close peak percentage Freeze Time minutes Time Specified in minutes that the spike sorting process of determining the number of units per channels runs Update Rate spikes Specifies the number of spikes that needs to be collected per unit before spike sorting statistics are updated Assume one unit per channel at the beginning Minimum Peak Percentage Histogram peaks have to have a minimum height which is calculate2. Log Sorting Changes Always F Log PCA coordinates Record Autoload System Settings Enable Autoload open dialog Rename eet Figure 20 Central Options o Allows multiple instances of various applications Do Not Log Sort Changes Will not automatically log sort changes o Log Sorting Changes on Record Will automatically log the spike sorting model the signal to noise ratio summary and the crosstalk summary if gets rebuilt during a recording session o Log Sorting Changes Always Will log the spike sorting model the signal to noise ratio summary and the crosstalk summary any time it gets rebuilt o Log PCA coordinates on Record Will log the spike sorting model only when recording is started Enable Autoload If this option is checked upon opening Central a pre determined CCF file will be opened and settings will be loaded If the field is left blank Central will allow the operator to select a CCF file o Prompt before load Will prompt the user before loading the CCF file The Windows menu Will have information on the system and the software as well as shortcuts to the various apps o This option displays the version of the software and the firmware of the NSP These version numbers may be needed if you contact technical support o System Summary Will open the System Summary window o Revision 4 00 LB 0175 Page 20 NeuroPort User s Manual
3. Figure 37 Spike Panel o Play When selected Raster Plot displays wrap o Scroll When selected Raster Plot displays scrolls o Pause When selected the Raster Plot display will pause Line per Channel When selected all spike events on a channel will be displayed on one line o Split Channel Splits single channels in the raster plot display A channel can be displayed by online classification rules and continuous waveforms Rate Lets the user select the scrolling speed at which the raster plot displays the data Set the range from 10 ms pix to 250 ms pix milliseconds per pixel o Decrease size Increase size Minimize and Maximize Channels Select a channel or group of channels in the raster plot to increase or decrease the size of the selected channels o Delete Channel Click this button after selecting a channel or group of channels to delete them from the raster plot display o Move Down Move Up Use these buttons to move one or a group of channels up or down in order on the raster plot display o Scale Continuous Data Use this button to display continuous waveform in the raster plot module and adjust the scale for display o Refresh Refreshes the raster plot display Sort This button sorts the channels in the raster plot display Click the drop down arrow to display the sorting types then select sort by Channel ID Recording Mode or Unit ID Choose Channels Add Clear All Click the Cho
4. Hardware Configuration To access this app click on Hardware Configuration on the Central app This app is for setting up channels and various other parameters It also allows the user to define the sampling frequency filtering parameters and many other features for each individual channel Further the user can set other global and spike sorting variables too Hardware Configuration EET Fie E Signal Types Chane Location Saraction Cone sampung Tunc Reject p SUH TSE Es Analog Outpt 82 Bank APin2 ye Digital in Bank Digital Out eens yo 4 Sank A Edel Pa ye e oa Eees Bank A yG HemtndAmp seco Bank A Ping ye Amoon Rank Analog Ou Bank A Pint ye Dui 0 Bank A Pini Digital Oe Bank A Pin 33 yes BwkA Pins M edes Banka PIS yes ded BankA PIE yes Settings Bank A PRI Globst settings Bank A Pin yes fite Bank PIS yer Auto Thresholdin 9600 Bank A 20 yes Spike Soring ye 92 Bank A Pn2 ye Nese Grape ded Bank A Pin 23 ye Sank A PM yes 5 Bank A Pim25
5. NC No Connocilor Figure 8 Headstage Assembly and Connections Front End Amplifier Input The FEA has four 34 pin banks Each bank consists of 32 channels a reference electrode pin and a ground pin The electrodes within each bank are differentially amplified with respect to the reference electrode input of the same bank If all electrode channels on all banks are to be electrically measured with respect to a common reference electrode this reference electrode needs to be connected to the reference input on every bank Revision 4 00 LB 0175 Page 13 NeuroPort User s Manual Figure 9 Front End Amplifier Input Schematic Additionally four 6 pins power supply banks provide power 45V to the headstage and are capable of delivering 130 of combined current to the The ground pins on every connector are internally connected to the grounding post on the back of the unit For best results the FEA should be the central grounding point for electrical ground connections to the research subject Cables Included with the system is a power cable for the NSP not shown a power cable for the APS not shown a fiber optic link that connects the FEA to the NSP a crossover Ethernet cable that connects the NSP to the host PC and cable that connects the APS to the FEA Figure 10 Fiber optic Cable Figure 11 Crossover Cable Revision 4 00 LB 0175 Page 14 Neuro
6. Bank A Pinas Bank A PAZ yu Sank A Pin28 yes Eee Sank A Pin29 eee Bank A Pin30 yes Edel Bank A Pin3t ves Eee BankA Pin 32 yes Baka Pn je 2 yes yes maka Find ya Mec mankupas ya 4 D Ready Figure 21 Hardware Configuration By default only one instance of Hardware Configuration be opened To open multiple instances enable this feature in Central under Tools gt Options All of the NSP inputs and outputs be grouped by function or by their location on the NSP Signal Types are grouped by function NSP Channels are grouped based on their physical location on the NSP The NSP Channels consist of Front End Amplifier Inputs Analog Inputs Analog Outputs Digital Inputs Digital Outputs Serial I O and Audio outputs The Hardware Configuration window contains a toolbar with buttons in the upper left corner to change the view of the window The buttons from left to right are Icon View List View Detail View and Full Row Details Each entry represents a probe or channel on the array and the neural signal being received To configure any of these channels select the channel type on the left side of the window Each individual channel is displayed on the right side of the window for the channel type that was selected You can configure the channels individually by groups of channels or select all cha
7. lt disabled gt z Source Channel eec o ce J Figure 24 Analog Output Properties Label A user defined name for the specific channel o Input and Output Range Fixed values for informational purposes o Track Most Recently Selected When this box is checked the channel source will change based on whichever channel is selected from Spike Panel Neural Channel or Raster Plot o Source Type Choose whether to output LFP or Spike data through the analog output Source Channel Select the channel to monitor through the analog output Digital In This window will allow the user to set the properties of the digital inputs being received from other equipment connected to the NSP Revision 4 00 LB 0175 Page 23 NeuroPort User s Manual Digital Input Properties Bank Digital Input 7 oci Cancel Apply Figure 25 Digital Input Properties Label A user defined name for the specific channel o Function The functions may include 16 bit on word strobe reads 16 bits on strobe 16 bit on bit changes reads 16 bits when any bits change falling or rising 16 bit on rising edge read 16 bits when any bits get set high 16 bit on falling edge read 16 bits when any bits get set low 8 bit strobe 8 bit bit changes reads 8 bits on strobe and the other 8 bits on any bit changes rising or falling 8 bit strobe
8. o Reset to Default Pressing this button will reset the X Y Z values above to default for all channels o Apply to All Pressing this button will apply the X Y Z values above to all channels Spike Sorting This window allows the user to specify the rules and settings used with the automatic spike sorting The default settings are recommended but can be changed to user preference Revision 4 00 LB 0175 Page 27 NeuroPort User s Manual XE E chum Ee rero ports ae projected orto an and a hatoga a BS Falet to agt vay delec Valey Percentage pal bn frt peak height valey percentage z Secord pad pa sut chmo nnd f ps ve hat gt port mean arand val dos pesk Ne dinis aay wig ar Freeze Tee J canes wets sake aan lard BG nete diea mec rp sail ane logro be oper UEM decise gt Peak Percentage valley height or zero total 2 samples used minimum peak percentage A awana A Com pem Maunsmdresaheren charrada re uta sana ar verse pce E Lael crane by phe Ok tool Ones samples onto wat parameters 05 05 0016 Figure 32 Histogram Peak Count Spike Sorting Properties propere Parameters
9. Neural Signal Simulator NSS Power Supply Power Cables and Connectors 1 Gbps Network Interface Card amp Crossover Ethernet Cable NeuroPort User s Manual amp Software Disk Revision 4 00 LB 0175 Page 9 NeuroPort User s Manual Hardware Front End Amplifier FEA The amplifier receives signals directly from the electrodes through a headstage or the patient cable The sampled signal up to 30 kHz is amplified analog filtered 1 order high pass at 0 3 Hz and 3 order low pass at 7 500 Hz and digitized 16 bits at 250 nV resolution and then is transmitted to the NSP via a fiber optic link The analog filter allows for the low frequency field potentials as well as the higher frequency spike signals to be recorded A later stage digital filtering will allow these two signals to be separated end amplifier Figure 2 Front End Amplifier The FEA ground is not connected to the ground pin of the AC connector There are ESD shunt circuits that will conduct differences of 1000 V or more The amplifier is isolated and floating Front End Amplifier Power Supply APS The power supply consists of five analog and digital supply channels with monitoring sequencing and emergency shutdown control In the event of an error in voltage or power delivery the power supply will shut down and a red error LED will turn on After checking all amplifier and patient cable connections for any inadvertent shorting turn off the power
10. ince they fom separate chess Channel dei Select specie channel Hvielen Zs Len 50 5 E Dinero ot the elipse Grex Conety Corti ro fo fe Location of cone ol elipse Rotation Angles Around Axes degrees Aud YA Around W sound mes Reset lo Dele aiota oe cem Figure 31 Auto Thresholding Properties Noise Boundary Threshold Multiplier The signal is filtered between 1 5 kHz 2 order Butterworth for spike detection and the noise in o that band is estimated The signal value has to cross the average noise times the Threshold Multiplier This value should be between 2 0 and 3 0 o X Y Z dimensions of the noise ellipsoid Radius of the Noise Ellipsoid in the feature space along each axis This parameter can also be set graphically by dragging the outside of the ellipsoid in the feature space view of the analog properties window o X Y Z center location of the noise ellipsoid The center point of the Noise Ellipsoid along the X Y and Z axis This parameter can also be set graphically by dragging the center of the ellipsoid in the feature space view of the analog properties window o 2 axis rotation of the noise ellipsoid The rotation angle of the Noise Ellipsoid along each axis This parameter can also be set graphically by dragging the rotation handles of the ellipsoid in the feature space view of the analog properties window
11. or Internet Protocol Version 4 TCP IPv4 6 Click on Internet Protocol TCP IP and click on Properties 7 For IP Address enter 192 168 137 1 for Subnet Mask enter 255 255 255 0 and leave the rest blank 8 Click on OK to save changes gO Control Panel Network and Internet Network Connections Organize Cisco AnyConnect VPN Client w Local Area Connection Connection LU Figure 15 Network Connections Window Revision 4 00 LB 0175 Page 16 User s Manual Local Ares Connection Propert Intemet Protocol Version 4 Properties wmm LL seve Connect using vaca get setings ane anat fu netek gprs 7 Broadcom tik TM Ge E D apy ured wad err erat RAE s Use the folowing IP address for Merosot Networks P adress 12 168 137 1 Fie and Prter Sharng tor Networks Subnet mas 255 255 255 0 LC temet Protocol Verson 6 TCP IP YE Lun acad L 02 UnicLayer Topology Discovery Mapper VO Diver L Topology Discovery Responder Obtan ONS server adress Use the flonng DNS server adresses Prefered DS sever peces Alternate DNS server Trensmsson Corta Protocol irtemet Protocol The defaut wide rea network protozoi that provdes acces dene ctercorneced Elo
12. or Raster Plot o Function Choose whether to output LEP or Spike data through the analog output Source Select the channel to monitor through the analog output Global Settings Revision 4 00 LB 0175 Page 25 NeuroPort User s Manual Global Settings Spke Properties Spike Width samples Prethreshold Samples Line Noise Cancellation Line Noise Frequency 60 Hz LNC Reference Channel elect X Cancel Apply Figure 29 Global Settings o Spike Properties Define variables related to the size of a spike triggered spike o Spike Width Allows a user definable number of samples to be collected in the spike triggered processing window The default value is 48 samples This would collect spike triggered waveforms for 1 6 ms The spike width can be set to a range of 30 samples up to 128 samples 1 0 ms to 4 3 ms spike triggered sample width The longer this value the larger the size of a spike triggered waveform o Prethreshold Samples The number of samples to capture before the threshold is crossed The default value is 10 meaning that 10 samples before the point of crossing threshold are captured o Line Noise Frequency The line frequency of the system either 50 Hz or 60 Hz This value changes in different countries For example line noise frequency in the Unites States is 60 Hz but it is 50 Hz in the Europe o Reference Channel The reference channel used to extract
13. 8 bit bit rising edge reads 8 bits on strobe and the other 8 bits when any bits get set high 8bit strobe 8 bit falling edge reads 8 bits on strobe and the other 8 bits when any bit gets set low o NOTE The strobbed input is read by the NSP on the rising edge of the strobe signal The user should set the data bits and then set the strobe bit high so that the NSP can capture the data bits Setting the strobe bit low will not trigger the NSP to read the data bits Digital Out This window will allow the user to set the properties of the digital outputs being transmitted to other equipment connected to the NSP Digital Output Properties General Selings Bank Digtal Ou Pin 1 we mo 7 Properties Moritor Electiode zl T Track Most Recently Selected Source Monitored Units 1 2 3 4 5 K Cancel Apply Figure 26 Digital Output Properties Label A user defined name for the specific channel o Properties The user has the option to Monitor Electrodes which will send a TTL pulse from that specific Digital Output every time a spike is detected or a specific sorting spike is detected As a second option the user can select Timed to output a user specified frequency range with a specified duty cycle and offset The low and high sampling show the number of clock cycles the square wave is set to h
14. Adaptive Filtering utility provides a means to determine how much of the recorded signal is comprised of noise so that parameters can be set to eliminate the noise from the signal Adaptive Filtering ielerence Channels fore dee E Ok Cancel Figure 34 Adaptive Filtering Plug the peripheral equipment into the NSP then click on Hardware Configuration gt Adaptive Filtering right click the channel icon then select Properties Use the drop down menu to activate the settings The Reference Channels can be set as 1 or 2 Channel filtering of spike and continuous data or just the spike stream data Click Apply to activate filtering The noise level is automatically computed by the system N Trode Groups An N Trode is set of electrode channels that can be chosen and assigned by the user The user can have multiple N Trode sets Each N Trode set can contain up to 4 channels When using a multi electrode arrays or an electrode shank with multiple recording sites this software allows the user to define the single channels associated to the multiple recording points and perform sorting on the N Trode as an entity To define N Trode groups open a group and double click on available channels to assign them to a group Press OK to save changes Properties wade EZ we Asse Stes Figure 35 N Trode Properties Spike Panel This app displays the detected spikes on all
15. Record For box checked specify an amount of time in seconds that the NeuroPort system will record data Once the time has expired the recording will stop automatically Start Stop Recording Press the Record button to start recording data Press the Stop button to stop the data acquisition cycle This is an acceptable way to stop acquisition cycles as it simply closes the data file and preserves all file content up to the point of acquisition termination Remote Recording Control Check the remote recording box to Start Stop Pause and Resume file recording from an external source through the digital input port or the serial I O port Click the Setup button below the remote recording check box to set user defined hex values for each remote recording function For example to start recording as soon as HEX value 0001 is read on the digital port and ignoring bits 5 12 choose Digital Input enter 000 for the first HEX box mask and enter 0001 for the second HEX box read value File Storage Options IF nate Sync Puse vert Sync Pase wh Gong Lon Remove Nowe Syne with Neurobotve recording present Fle Spir Optons TOC mode oniy Spit Fle interval TES Seconds an estmate of he cntruocs Seset sae based on he st tena The abore You can spronmate the te neal to sit based on requested eset re by entermg Se requested Reset soe rd doing on Tine Requested 6 curent contr
16. for use in the electromagnetic environment specified below The customer or the user of the NeuroPort amp system should assure that it is used in such an environment IEC 60601 test Compliance Immunit unity test level level Electromagnetic environment guidance Portable and mobile RF communications equipment should be used no closer to any part of the NeuroPort system including cables than the recommended separation distance calculated from the equation applicable to the frequency of the transmitter Recommended separation distance Conducted RF 3 Vrms d 124P 150 kHz to 80 MHz 61000 4 6 150 kHz to 80 MHz d 124P 80 MHz to 800 MHz Radiated RF 3 V m 3 V m IEC 61000 4 3 80 MHz to 2 5 GHz d 2 34P 800 MHz to 2 5 GHz where P is the maximum output power rating of the transmitter in watts W according to the transmitter manufacturer and d is the recommended separation distance in meters m Field strengths from fixed RF transmitters as deter mined by an electromagnetic site survey should be less than the compliance level in each frequency range Interference may occur in the vicinity of equipment marked with the following symbol NOTE 1 At 80 MHz and 800 MHz the higher frequency range applies NOTE 2 These guidelines may not apply in all situations Electromagnetic propagation is affected by absorption and reflection from structures objects and people Field streng
17. how many spikes are displayed before the screen is refreshed Show Spikes Drop Menu You may specify which waveform types are displayed in the spike Display Waves Pattern View spikes in waveform or 2D or 3D pattern view Cont Signal This allows the display of the continuous waveform signal and spike display in raster format Raw LNC Click this button to display the raw data and any applicable line noise Manual Override options A user can override the automatic spike sorting on individual channel by clicking on the Pattern view Click the Edit button next to the classified spike to override the auto spike sorting function for that sorted unit and resort to a manual sorting option Press lt C gt to see all available controls Hold down the lt Ctrl gt key and draw an ellipse in the Pattern view around the cluster that you would like to define as a spike You may also change the size of the ellipse to make the diameter larger or smaller Once the ellipse is placed in the cluster that you are defining the word Overridden will appear next to the sorted unit You can also change the threshold radius by changing the diameter of the grey ellipse in the center of the Pattern Display Any units that occur in the grey circle will not be displayed as a spike 3D PCA On line Spike Classification If manual PCA is selected spike sorting is performed using online PCA analysis within the PCA space PCA Spike Sorting can clas
18. in its calculations Analog Input This window will allow the user to set different properties of a specific or a group of selected analog input channels the options are the same as the ones of the front end amplifier inputs except that the coupling can also be determined for each analog input channel Revision 4 00 LB 0175 Page 22 NeuroPort User s Manual Analog Input Properties DEC _ Location Enable Spike Extraction BakAnmkgin 20000 Analog In a w ERN None Digtal Rangs 32767 wo rre Enable Continuous Analog Range 5000 to 5000 0 150 x Analog mv Extemal Ga 1 I Enable Line Noise Cancellation 7 Enable Amplitude Rejection w F 15000 SES _ _ IV AC Input Coupling 2 Spike View Raster View App Cancel seem Figure 23 Analog Input Properties o AC Coupling The coupling for each analog input channel is user selectable between AC and DC coupled By default channels 1 8 are AC coupled and channels 9 16 are DC coupled Analog Output This window will allow the user to set the properties of the analog outputs being transmitted to other equipment connected to the NSP ing Cup Popes Bank Analog Out Pin 1 Labet aout Input Range 5x0 fe Output Range Foo es Track Most Recently Selected Type
19. kHz 30 kS s Samples data at a rate of 30 000 samples per second and digitally high pass filters it at cutoff frequencies of 250 Hz This option is typically used for recording high frequency action potential data Revision 4 00 LB 0175 Page 18 NeuroPort User s Manual Central This is the main control app To run Central click on Start navigate to the Blackrock Microsystems folder and click on the Central shortcut This app has shortcuts to all the other apps in the suite Clicking on each icon will open the corresponding app Clicking Reset will reset the timestamps on the NSP and sets it back to zero Central 77 File Tools Windows Hardware Configuration Spike Panel Flaster Plot jingle Neural Channel Activity Map File Storage Signal to Noise Ratio Neural Modulation Thresholding Impedance Tester Crosstalk System Load 2044 MByte s 12 5 D Pts Sent 4868 Pkts Received 72328355 Reset Running 0 Jj Figure 18 Central Main Application The File menu contains functions to load amp save various configuration files as well as the option to close applications and shut down the NSP hardware Load System Settings Load previously saved channel settings Load Sorting Rules Load previously saved system calculated sorting rules Load PCA Basis Load previously saved system calculated PCA basis vectors Save System Settings Saves
20. marked channels can flag any channels as bad and disable them from the Edit drop down menu N Trode The N Trode utility is used when electrode types with multiple recording points are located on the same shank or in the same discrete area of an electrode array Mw n Mh Figure 46 N Trode Utility o Before being able to use this utility N Trode groups need to be defined in Hardware Configuration as described in N Trode Groups Revision 4 00 LB 0175 Page 38 NeuroPort User s Manual o 29 o nTrode Use the drop down menu to switch between the different N Trode groups You can also use the arrows to flip through the various groups o Hide Continuous Checking this box will hide the continuous signal window on the bottom of the page to leave more room for the N Trode display boxes o Magnification It changes the magnification of the pattern window by zooming in and out o Define Delete Units Select a unit then click on Define Hold down the Ctrl key and using the mouse to draw a shape around a given cluster to define it as a unit Repeat this process until all units are defined To delete select a unit and click on Delete button Revision 4 00 LB 0175 Page 39 NeuroPort User s Manual Other Utilities Central Play CentralPlay is a tool for replaying pre recorded digital data NEV file type back for review and re sorting using hoops A recorded video may a
21. of the front end channels Double click on any channel to display its events in the Single Neural Channel window explained later Use the Shift or Ctrl keys to select several channels to highlight Channel selection and other display properties can be turned on or off through the right click context menu A map file can be applied to the display to change the spatial arrangement of the channels on the Revision 4 00 LB 0175 Page 29 NeuroPort User s Manual screen to match the electrode array in use Figure below displays channels of a 10x10 electrode array If spike sorting rules are defined the spikes belonging to different units will be displayed in different colors gum Bl Spice Parei View Hep Aat Never v 1 Ta 2255 y Figure 36 Spike Panel Load Map File The default map file for the spike panel is the 16x8 A custom map file can be loaded to change the layout to match the spatial arrangement of the channels on the screen to match the electrode array in use Contact Blackrock Customer Service to obtain the map file specific to your electrode array Load Default Map File By selecting this button the default 16x8 grid of 128 channels will be loaded o Map File Information Displays information about the map file currently loaded o Time to screen refresh The drop down tool allows the user to select the time until the spike panel display will refresh The refresh rate
22. recorded using the 16 bit DB37 input port The pin diagram is shown below DS is the digital strobe pin D0 15 are data pins EOP is reserved SYNC is an output pin and can be used with external equipment to indicate when the port is scanned DS Di 02 D4 DS DS DS 010 011 012 013 014 015 EOP SYNC All of the lower pine connected to digital ground Figure 6 Digital In Pin Diagram 5 Serial I O The port is RS232 DBO digital input output port The pin diagram is shown below Currently software only supports this port as an input Pin 2 is Receive Data pin 3 is Transmit Data and pin 5 is Ground Revision 4 00 LB 0175 11 NeuroPort User s Manual 22000 0900 Figure 7 Serial O Pin Diagram 6 Analog Outputs Four 45 0 V analog output BNC connectors can be used to send monitoring signals or stimulus waveforms to other connectors 7 Audio Output The system sends a 1 V line level audio signal of the selected data channel to two BNC ports and one 3 5mm female stereo audio connector 8 Digital Outputs Four single bit digital BNC outputs can be programmed for monitoring or timing functions 9 Sync Port A synchronization pulse can be set as an optional line to inform equipment when the NSP neural signal inputs and front panel ports are scanned It is active on the rising edge of the signal Fiber optic Link This port connects to the FEA using a fiber o
23. the files The numeric file name indicated the data and time of recording For example 20110124 152145 indicated that the data file was recorded on January 24 2011 at 3 21 45 PM File Storage will also save the settings in the same folder as the data file at the beginning of a recording session Revision 4 00 LB 0175 Page34 NeuroPort User s Manual na pel vf su Name fadni coya Storage Feder tone Ogos Bronte T Recent 5 see Elapsed oumens S angle Recordings T Remate Recording Control Recorded Sphe Count Ween iaa Domeni Sang Recodrg 2 S Packet Cort deccm r Fi Sit Fe Sos Avaloie M6 931 sel Tine 154216 Figure 40 File Storage File Splitting The software can split recorded data files during a recording session to allow smaller file sizes for offline analysis The file size and time limit before the system will create a new file can be user defined by clicking the view drop down menu Options To disable file splitting check the disable file splitting box in the file storage module File Description Use the dialog to add comments up to 256 characters long to the data files Multiple lines can be entered by pressing the lt Enter gt key but the user should not enter more than eight lines in this field Comments need to be entered prior to recording a data file Record For With the
24. the line noise The extracted line noise for each channel can be viewed in Single Neural Channel when the Raw LNC button is checked Filters This window provides specifications of the various filters set in the NeuroPort system The filters can be set for each channel individually For graphical details and specifications click the digital filter dropdown box and select a filter Fiter Properties Lo Hoh Pass ropes Low Pas Mh Pas Low Pass Popes Fae Tie Buenos uere amas 53 90 Come ORE Come Fea SURE De f ode f D Teri End Ane Carte Anbar Chara Figure 30 Filter Properties Revision 4 00 LB 0175 Page 26 NeuroPort User s Manual Auto Thresholding This window allows the user to specify the auto thresholding properties for spike detection sa Auonated Threshold Stings Theetholdeg i done on a processed version o the signato low forthe ol ver smal Note The capture cl some noise necessary lo delet smal unts For this exa ol dening a boundary was added Thecheld Midler vals esse estende thesheld miter Aucnated may ead n lake postive pke detechons whch are by noise elipse in the spl sorting estre space wil unuq not the deletion ol malspker
25. CENTRAL OPTIONS FIGURE 21 HARDWARE CONFIGURATION FIGURE 22 FRONT END INPUT PROPERTIES FIGURE 23 ANALOG INPUT PROPERTIES FIGURE 24 ANALOG OUTPUT PROPERTIES FIGURE 25 DiGITAL INPUT PROPERTIES FIGURE 26 DIGITAL OUTPUT PROPERTIES FIGURE 27 SERIAL 1 PROPERTIES FIGURE 28 AUDIO PROPERTIES FIGURE 29 GLOBAL SETTINGS FIGURE 30 FILTER PROPERTIES FIGURE 31 AUTO THRESHOLDING PROPERTIES FIGURE 32 HISTOGRAM PEAK COUNT SPIKE SORTING PROPERTIES FIGURE 33 MANUAL PCA SPIKE SORTING PROPERTIES FIGURE 34 ADAPTIVE FILTERING FIGURE 35 N TRODE PROPERTIES FIGURE 36 SPIKE PANEL FIGURE 37 SPIKE PANEL FIGURE 38 SiNGLE NEURAL CHANNEL FIGURE 39 ACTIVITY MONITOR FIGURE 40 FILE STORAGE FIGURE 41 FILE STORAGE OPTIONS FIGURE 42 SIGNAL TO NOISE RATIO FIGURE 43 NEURAL MODULATION FIGURE 44 AUTO IMPEDANCE Revision 4 00 LB 0175 NeuroPort User s Manual FIGURE 45 CROSSTALK Figure 46 N TRODE UTILITY Revision 4 00 LB 0175 Page4 NeuroPort User s Manual mbols of Contraindications Warnings Cautio Danger of IEC60101 0102 Electrostatic 1 1 60417 5019 Discharge ESD ground Dangerous E Equipotentiality 1EC60417 5036 voltage IEC60417 5021 Car
26. GHTS Blackrock SHALL NOT BE LIABLE FOR SPECIAL INDIRECT INCIDENTAL PUNITIVE EXEMPLARY OR CONSEQUENTIAL DAMAGES INCLUDING WITHOUT LIMITATION DAMAGES RESULTING FROM LOSS OF USE LOSS OF PROFITS INTERRUPTION OR LOSS OF BUSINESS OR OTHER ECONOMIC LOSS ARISING OUT OF NON COMPLIANCE WITH ANY WARRANTY Blackrock s ENTIRE LIABILITY SHALL BE LIMITED TO PROVIDING THE REMEDY SET FORTH IN THE PRECEDING PARAGRAPH Return Merchandise Authorization RMA In the unlikely event that your NeuroPort system needs to be returned to Blackrock for repair or maintenance do not send any equipment back without a Return Merchandise Authorization Number An RMA number will be issued to you by a Blackrock representative If you need to obtain an RMA number you may contact a product support representative at 801 582 5533 or toll free at 866 806 3692 Once an RMA number has been issued it is important to safely pack the returned item for shipping back to Blackrock It is preferred that you save the original boxes and packing materials that your NeuroPort system arrived in for return shipment Please address the package as follows Blackrock Microsystems ATTN RMA 391 Chipeta Way Suite G Salt Lake City UT 84108 USA Tel 801 582 5533 Revision 4 00 LB 0175 Page 42 NeuroPort User s Manual Troubleshooting Extraneous Noise You will find that the NeuroPort system is very sensitive to extraneous electromagnetic signals It is designed
27. NeuroPort Bio potential Signal Processing System User s Manual Revision 4 00 Ge 22 2 No 601 1 22 2 No 801 226 BLACKROCK MICROSYSTEMS 391 Chipeta Way Suite Salt Lake City UT 84108 866 806 3692 www blackrockmicro com support blackrockmicro com Caution Federal law USA restricts this device to sale by or on the order of a licensed physician NeuroPort User s Manual Table of Contents TABLE OF CONTENTS TABLE OF FIGURES SYMBOLS OF CONTRAINDICATIONS WARNINGS CAUTIONS CONTRAINDICATIONS WARNINGS CAUTIONS SPECIFICATIONS INTRODUCTION SYSTEM DESCRIPTION SYSTEM REQUIREMENTS SYSTEM SCHEMATIC PACKING CONTENTS HARDWARE FRONT END AMPLIFIER FRONT END AMPLIFIER POWER SUPPLY APS NEURAL SIGNAL PROCESSOR NSP CONNECTORS CABLES ETC Headstage Requirements ICS Connectors Front End Amplifier Input Cables Neural Signal Simulator 55 1 Gbps Ethernet Card SETUP HARDWARE SETUP SOFTWARE SETUP DATA ACQUISITION SOFTWARE NEUROPORT CENTRAL SUITE Installation Sorting Algorithms Sampling Rates CENTRAL HARDWARE CONFIGURATION Front End Amplifier Inputs Analog Input Analog Output Serial 1 0 Revision 4 00 LB 0175 Page 1 NeuroPort User s Manual Global Settings Filters Auto Thre
28. Port User s Manual Figure 12 Amplifier Power Supply Cable Neural Signal Simulator NSS The NSS supplies simulated field potentials and action potential activities It can be used to test the system in lieu of a connected patient The NSS is powered by four AA batteries that can be accessed by removing the back screws and the back plate The black pin is NSS Ground Figure 13 Neural Signal Simulator 1 Gbps Ethernet Card The supplied Ethernet Card is to be installed in a 64 bit PCI slot of the Host PC It will connect to the NSP via the supplied Ethernet cable Figure 14 I Gbps Ethernet Card Revision 4 00 LB 0175 Page 15 NeuroPort User s Manual Setup Hardware Setup The supplied 1 Gbps PCI Ethernet card needs to be installed in a 64 bit PCI slot of the host PC and a crossover Ethernet cable included is needed to directly connect the host PC to the NSP The host PC and the NSP can also be connected through a 1 Gbps Business Class Ethernet switch not a router or a hub in which case a regular Ethernet cable is needed Software Setup 1 Once installed the card needs to be configured to connect to the NSP 2 Windows XP Go to Control Panel Network Setup 3 Windows 7 Click on Start and search for View Network Connections 4 Right click on the correct Ethernet Adaptor usually Local Area Connection and click on Properties 5 Uncheck all services except for Internet Protocol TCP IP
29. atch the headstage gain value Amplitude Rejection Rejects any waveform that falls outside a user defined amplitude Spike Extraction When enabled default the system will display the spike triggered events Sorting Algorithm Sets the sorting algorithm for a channel as described in Sorting Algorithms on page 18 Continuous Acquisition By enabling this option the NSP records continuous waveforms by selecting a bandwidth The appropriate sampling rate for the continuous waveforms is automatically selected with the corresponding bandwidth Continuous Acquisition Bandwidth Sample Rate Sets the sampling rate for a channel as described in Sampling Rates on page 18 Line Noise Cancellation This enables the line noise cancellation LNC filter With the LNC filter enabled you can set the time constant in the drop down menu to adapt to the 50 or 60 Hz line noise A time constant of 10s is the default and recommended setting However you may set the time constant from 15 to 60s instantaneous or to hold Reference Electrode In addition to the hardware reference this selection allows you to specify a software reference electrode The software reference can be applied to either the raw data stream which affects both LFP and spike data or it can be applied to only the spike data stream The artifact rejection algorithm works only when all channels are enabled For instance even if 80 channels are inactive the algorithm will still use those channels
30. can be set to refresh every 2 5 10 or 20 seconds or Never to stop the refresh Open Selected Channels After selecting a channel or group of channels to display in the neural channel module click this button to open the selected channels or right click after selecting channels then click Open Selected Channels o Refresh Screen Select the refresh button to manually refresh the spike panel display o Magnify Current Channel Select this button to magnify the channels in the spike panel Move the mouse over the channel to magnify the channel When magnify is turned on the channels will only be magnified if the spike panel display window has been sized down o Broadcast Channel Selections Select this button broadcast the selected channels to other computers that may also be connected to the NeuroPort system through an Ethernet connection o Level of Criticality LOC The LOC button displays the load on the PC Raster Plot The Raster Plot screen displays spikes as a pixelized image each pixel representing a signal impulse sampled for the length of time set by the user in the drop down box In this example 30 ms pix indicates that each pixel indicates a neural signal occurring in a 30 millisecond time period Continuous analog data can also be shown Revision 4 00 LB 0175 Page 30 NeuroPort User s Manual TB Raster Piot View y Dw ee 100 suec z None 18 8 28 28 X BH 0 50 3 Mi 3e 88
31. d by adding the valley height or zero to the value of total of samples used minimum peak percentage Method Manual PCA Uses Principle Component Analysis method to sort units recorded on an electrode Wave Basis Size The number of waveforms to use to calculate the PCA basis vectors Example 250 waveforms are needed to create the PCA basis vectors Update Basis Multiplier The multiplier of the basis size This number is used to recreate the basis after so many waveforms For example if this number is 5 and the Wave Basis Size is 250 then after 1250 250 x 5 waveforms the PCA basis vectors are recalculated Revision 4 00 LB 0175 Page 28 NeuroPort User s Manual o Artifact Rejection This feature is used to get minimize the amount of artifact in the signal o Maximum Simultaneous Channels If more than X channels fire at the same time their events are rejected and the waveforms will not get recorded o Refractory Period Spike detection is turned off for X samples after an event has been detected The artifact rejection algorithm works only when all channels are enabled For instance even if 80 channels are inactive the algorithm will still use those channels in its calculations Adaptive Filtering Recorded signals may contain some noise from any known sources of external noise such as an eye coil box These external noise sources may be plugged into the NeuroPort System and tested for extraneous noise levels The
32. die segs ment pe Cox _ _ Figure 16 Local Area Connections Properties Figure 17 IPv4 Properties Note Up to 16 PCs can be connected to the same NSP using a 1 Gbps network switch For other PCs connected using a network switch IP addresses should increment such as 192 168 137 2 192 168 137 3 etc Note For reference the NSP s IP address is 192 168 137 128 Revision 4 00 LB 0175 Page 17 NeuroPort User s Manual Data Acquisition Software NeuroPort Central Suite Installation To install the software suite run NeuroPort Central Suite msi from the supplied CD and follow the instructions The NeuroPort Central Suite consists of several applications that perform various functions To run these apps first run the Central shortcut via the Start Menu Sorting Algorithms The program can sort detected action potentials using three different methods o o o Hoops This is a manual method A time amplitude algorithm which involves manually placing hoops where the detected spike waveform must go through Manual PCA This is a manual method The system calculates the first three principle components on a representative sample group then projects each new waveform into the same 3D PCA space The user draws an ellipsoid around each cluster to sort the group Histogram Peak Count This is an automatic method An automatic sorting algorithm is described in Spik
33. do Type of CF 1EC606417 5335 Applied Part 1EC60417 5007 ON power 1EC60417 5008 OFF power 1 60417 5010 ON OFF push push CSA 60950 1 03 Class 1 Laser CLASS 4 LASER PRODUCT ipsun diia SN EN 980 Serial Number e EN 980 Manufactured date EN 980 EC Representative Combined 980 Manufacturer EN 980 Manufacturer Manufacturing Date Read the A EN 980 Warning EN 980 documentation LOT EN 980 Batch Contraindications The NeuroPort System is a recording system and should not be used in applications involving stimulation Warnings Only connect NeuroPort amp System components to properly tested grounded and dedicated AC outlets to reduce the risk of electrical shock Do not use an adapter for ungrounded wall outlets Do not connect the NeuroPort System to an outlet controlled by a wall switch Do not use the NeuroPort System in the presence of flammable anesthetic agents Avoid strong static discharges from sources like televisions or computer monitors because it can damage the electrical components of the system Keep the NeuroPort amp System away from liquids Contact with water shower spray or wet surfaces can lead to the patient receiving an electrical shock Connection of external instruments may compromise electrical safety compliance with IEC 60601 1 The NeuroPort amp System should be disconnected from any electrodes during cardiac defibrillation Ca
34. e Sorting on page 27 Sampling Rates The NSP is capable of sampling data at a rate of up to 30 kS s The recorded signal is band pass analog filtered by the amplifier at cut off frequencies of 0 3 Hz and 7 5 kHz Additionally depending on the configuration the signal is digitally filtered as described below o 0 3 Hz 150 Hz 1kS s Samples data at a rate of 1 000 samples per second and digitally low pass filters it at a cutoff frequency of 150 Hz This option is typically used for recording of continuous low frequency EEG ECG EOG ERG LFP waveforms 0 3 Hz 250 Hz 1 kS s Samples data at a rate of 1 000 samples per second and digitally low pass filters it at a cutoff frequency of 250 Hz 10 Hz 250 Hz 1 kS s Samples data at a rate of 1 000 samples per second and digitally band pass filters it at cutoff frequencies of 10 Hz and 150 Hz This option is typically used for recording of continuous EMG EEG waveforms 0 3 Hz 500 Hz 2 kS s Samples data at a rate of 2 000 samples per second and digitally low pass filters it at a cutoff frequency of 500 Hz 0 3 Hz 2 5 kHz 10 kS s Samples data at a rate of 1 000 samples per second and digitally low pass filters it at a cutoff frequency of 2 5 kHz 0 3 Hz 7 5 kHz 30 kS s Samples data at a rate of 30 000 samples per second This configuration does not digitally filter the signal This option is typically used for recording wide band raw data 250 Hz 7 5
35. gram Files Blackrock Microsyst Folders 4 awana CO Blackrock Merosystens E Config Ms E coverty Cyberkinetics 9 crown 9 ED Documents and Settings 2 dies 8 nremedote waw NCSvI 07 0914 m C3 Progam Fes sa Lal Myeuheve probleme wth tit program ard wokad coneciy on eser version of Windows the compatibliy mode matches that easier version Compatibilty mode Run this program n compalibliy mode lor C Run in 640 x 480 screen resolution C Disable visual themes Input setings Tum ell advanced le services fot this program Leam more about program conpatiblt No Power to NeuroPort System Tn the event that the NeuroPort system does not power on check the following 12 Verify that the NeuroPort amp system is plugged into a working outlet or power strip and verify that all power cords are plugged into the NeuroPort Revision 4 00 LB 0175 Page 43 NeuroPort User s Manual 13 Verify the position of the power switch and make sure it is in the ON position If the power switch is in the ON position turn it to the off position then wait at least five seconds before turning the NeuroPort back on It is recommended that when restarting the NeuroPort always wait five seconds before turning the unit back on This will ensure a proper s
36. hy EMG electrocardiography ECG electrooculography EOC and evoked potentials EP System Description The NeuroPort System is designed to record and process neural signals from up to 256 surface or penetrating electrodes using two synchronized NSPs in addition to analog and digital experimental events The system is capable of performing several online processing of neural signals including noise cancellation adjustable digital filters simultaneous extraction of spike and field potentials and manual and automatic online spike sorting System Requirements Microsoft Windows XP or Microsoft Windows 7 Professional Edition AMD or Intel 2 0 GHz Dual core CPU or faster 2 GB of RAM 1x free 64 bit PCI slot Minimum of 500 GB 3 Gbit s SATA II HDD System schematic Experiment Subject Area Experiment Operation Area Neural Electrodes with oss etd leural Electrodes with less and Digital than 1Megohm Impedance Experiment System Operator Signals n 1 P Front End 8 eue 1 Follower OR Front en 8 Cerebus Interface S od Headstages 8 Signal Software running Electrodes L 5 Processor on Users PC 2 Neural Signal Simulator for System Testing Figure 1 System Overview Packing Contents Neural Signal Processor NSP and Rubber Feet Rack Mounting 2 Ears and Screws 4 Front End Amplifier FEA amp Power Supply APS
37. icrosystems If you are using an ICS array holder without a headstage you will need to use a HBA 32 headstage bypass adaptor in place To use the ICS 96 you will need a group of three CHA 32 adapters that are mounted together with a shared reference To select Ref 1 or Ref 2 on the ICS 96 you will need to place a reference jumper on the CHA 32 board connected to Bank A or Bank C The connector board has up to four Samtec FTSH 36 pin connectors pins 1 32 are the electrodes pin 33 is the teference pins 34 and 35 is ground and pin 36 is bus It is connected to either 1X headstage followers HSF 32 or Headstage bypass adapter boards HBA 32 and then connected to the inputs of the amplifier with Samtec ribbon cables J1 to A J2 to B etc Revision 4 00 LB 0175 Page 12 NeuroPort User s Manual Ics 96 Connector Array Ref2 oz SIR Eu zm ONS 4 B 8 10 12 14 18 18 20 22 24 26 28 30 32 bal HBA 32 High Density CHA 32 Cerebus Front End or HSF 32 Ribbon Cable Adapter Ground ZSTMM 117 69 8 D 280 FTSH 118 04 L DV O OOO SFMC 118 L1 8 D SQT 117 01 L D FTSH 120 01 L D K A B C D Y FESD FH EIE 5 o 1 34 Jumper for H reference 108 08 5 0 I 10 pelecrion 8GT 103 01 L D 13 14 d 520 3 2528 Legend 7 28 R Reference 330 Ground tV 5 Volts V 5 Volts Evi bs Bus
38. igh or low The offset unit is clock cycles and can offset the trigger of one channel from the other output channels o Track Most Recently Selected When this box is checked the channel source will change based on whichever channel is selected from Spike Panel Neural Channel or Raster Plot Revision 4 00 LB 0175 Page24 NeuroPort User s Manual Source Select a source channel to monitor o Monitor Units The user can select which units to monitor from the source channel through the digital output ports The user can monitor unclassified units and or classified units defined online Serial O This window will allow the user to set the properties of the outputs being received from other equipment connected to the NSP via the serial ports Serial VO Properties ES Enable Channel Bank Serial 1 0 1 Labet senal Baud Rate 115200 Data Bits 8 Stop Bit 1 Figure 27 Serial I O Properties Audio This window will allow the user to set the properties of the audio outputs being transmitted to other equipment connected to the NSP T Track most selected Function cdisabled elect Dk Cancel Apply Figure 28 Audio Properties o Label A user defined name for the specific channel o Track Most Recently Selected When this box is checked the channel source will change based on whichever channel is selected from Spike Panel Neural Channel
39. io gt 90 dB at 50 60 Hz Neural Signal Input Sample Rate 30 000 Samples second Common Mode Input Range up to 3 0 V between inputs and ground Experimental Analog Inputs Sixteen 5 V 16 bit inputs for experiment or neural signal processing Differential Input Range up to 3 0 V between electrode and reference inputs Maximum Input Voltage Range up to 5 0 V between any input and ground Crosstalk between channels 1 LSB for all configurations Filter characteristics 15 order Butterworth high 3 order Butterworth low Experimental Digital 1 0 One 16 bit input port DB 37 with Word and Packet Strobe control lines One RS232 1 0 port DB 9 with 115k baud input and output Four single bit digital outputs BNC with programmable monitoring functions One TTL output BNC sampling synchronization output port High Pass Cutoff Freq 0 3 Hz full bandwidth mode Experiment Analog Outputs Four 5 V 600 ohm 16 bit outputs for monitoring and stimulus waveforms Low Pass Cutoff Freq 7 5 kHz Audio Outputs Two 1 V line level outputs Headstage Power Supply 35 0 V output up to 150 mA for powering optional headstages Multi NSP Synchronization From PC trigger to digital or serial input ports Control Data Output Connection MT RJ fiber optic port with 2 way 150 Mbps 88 10B encoded data stream and 32 bit CRC data validation PC Hardware Inte
40. kOhm 161 161 kOhm kOhm kOhm 161 1 161 161 kOhm kOhm kOh 0 kOhm kOhm 161 kOhm kOhm kOhm 161 kOhm kOhm kOhm kOhm kOhm NUM Figure 44 Auto Impedance 1 Click on Impedance Tester on Central The Auto Impedance app will open If data is being recorded the user will be prompted to stop recording or cancel the impedance test The user will also be given the chance to stop the recording from the dialog 2 A dialog box will be displayed notifying that Auto Impedance is checking the impedance switch to ensure it is in the Impedance position If not the user will be prompted to turn the switch to Impedance mode 3 Auto Impedance checks the array reference wires before beginning 4 Impedance data is collected then the Auto Impedance screen is displayed channels are measured simultaneously The measurement takes about one minute Impedance readings are displayed for every channel Tolerance is defaulted to 100 800 Q If any channels display numbers outside tolerance the box will turn yellow below or red over to indicate out of range values 5 Open View Options to display the Impedance Calculation Settings 6 Open the Edit menu or right click one of the bad channels to display the properties menu 7 Select Disable Bad Channels to mark out of tolerance channels with a display border 8 When the other Central modules are opened the disabled channels will be blank or contain an X thr
41. lect 3 39 25 00 35 i u eect 4 29 37 305 1013 M elect 5 25 28 3114 elec12 1 39 20 46 504 M el 2 13 35 2144 1469 elec12 3 1 283 2053 1378 i elci 4 25 313 2815 1112 elec12 5 29 294 2756 1031 EY iG wau z 23 340 733 E E ZISISSSU IIT Figure 42 Signal to Noise Ratio Neural Modulation Neural Modulation displays changes over time of the firing frequency The Modulation Index covers a range of 0 to 12 0 but a range of 2 5 is considered normal Bl Neural Modulation cak File View Help 3 9 Modulation Index 019 Seconds 19 Figure 43 Neural Modulation Impedance Tester Impedance Tester analyses each channel to ensure proper impedance of every electrode When selected a dialog box is displayed instructing the user to turn the impedance switch located on the patient cable to the impedance position marked with a sign Data cannot be recorded when the system is in impedance mode The impedance Revision 4 00 LB 0175 Page 36 NeuroPort User s Manual function can read impedances of about 50 or greater If an impedance measurement is below 50 an information box will pop up on the screen stating that the electrode impedance is below 50 k but will not give a number Steps to measure impedances are states below Ato impedance tdt View Hep 1 kOhm 161 161 kOhm kOhm kOhm kOhm kOhm 161 161 161 kOhm
42. lso be played back in sync with the recorded data To run click on Start and navigate to the installation folder of NeuroPort Central Suite and click on the CentralPlay shortcut A clock with a sliding bar allows the user to pause rewind and replay the data file The only editing capability of CentralPlay is the ability to define new hoops sorting rules to the recorded spikes Central Sim CentralSim is a tool for simulating data collection It is mostly used for demoing the software package and or its features To run click on Start and navigate to the installation folder of NeuroPort amp Central Suite and click on the CentralSim shortcut Revision 4 00 LB 0175 Page 40 NeuroPort User s Manual Hardware Specifications NEUROPORT FRONT END AMPLIFIER DIGITIZER NEUROPORT NEURAL SIGNAL PROCESSOR Input Range 8 095 mV Analog to Digital Conversion 16 bit digital output with 0 25 uV per bit resolution Input Impedance gt 10 ohms 3pF Input Bias Leakage 5pA typical 20 max Digital Signal Processing Adaptive Line Noise Cancellation filter 4 order hi lo pass digital filtering for all channels Separate filters for simultaneous continuous and spike streams along with online spike classification using time amplitude and or template matching Input Referred Noise lt 3 0uVrms 14uVp p Neural Signal Inputs Up to 128 Channels Common Mode Rejection Rat
43. ning a minimum distance between portable and mobile RF communications equipment transmitters and the NeuroPort system as recommended below according to the maximum output power of the Separation distance according to frequency of transmitter m Rated maximum 150 kHz to 80 MHz 80 MHz to 800 MHz 800 MHz to 2 5 GHz output power of 35 1 transmitter W d V MP d d Tg WP 1 0 01 0 12 0 12 0 23 0 1 0 38 0 38 0 73 1 1 2 1 2 23 10 3 8 3 8 7 3 100 12 12 23 For transmitters rated at a maximum output power not listed above the recommended separation distance din metres m can be estimated using the equation applicable to the frequency of the transmitter where Pis the maximum output power rating of the transmitter in watts W according to the transmitter manufacturer NOTE 1 At 80 MHz and 800 MHz the separation distance for the higher frequency range applies NOTE 2 These guidelines may not apply in all situations Electromagnetic propagation is affected by absorption and reflection from structures objects and people Revision 4 00 LB 0175 Page 8 NeuroPort User s Manual Introduction The Blackrock NeuroPort Bio potential Signal Processing System supports recording processing and displaying bio potential signals from supplied electrodes Bio potential signals may include Electrocorticography ECoG electroencephalography EEG electromyograp
44. nnels To configure an individual channel double click the channel to display the channel properties To configure a group of channels hold down the Shift or Ctrl and click on channels Once the group of channels to configure has been selected click the Channel Properties icon located at the top right side of the toolbar Revision 4 00 LB 0175 Page21 NeuroPort User s Manual Front End Amplifier Inputs This window will allow the user to set different properties of a specific channel or a group of selected front end amplifier channels f jes Analog Input Properties Location Enable Spike Extraction Bank FrontEndA Pin Sorting Algorithm Labet Histogram Peak Count 17 Enable Continuous Acquistion Digtal Range 39764 32764 Bandwidth Sample Rate Analog Range 8191 to 8191 250 75 30kS s Analog Unite 07 Graphical Chooser Extemal T T Enable Line Noise Cancellation T Enable Amplitude Rejection 15 Disabled X 2 Spike View Raster View Apply Cancel sever emen Figure 22 Front End Input Properties o Label A user defined name for the specific channel When configuring a group of channels you can label them on 0000000 global basis by clicking the Edit button Digital and Analog Range Fixed values for informational purposes External Gain Set the gain to m
45. o SH Ca 229 Figure 41 File Storage Options Enable Sync Pulse Will send a sync Digital Output 1 The sync signal is a unique waveform pattern and repeats itself for 14 seconds Each 14 seconds the pattern changes This can be used to synchronize recordings with an external equipment Invert Sync Pulse It will invert the sync pulse The pulse will start high and will go low Remove Noise Enabling this option will remove noise classified waveforms unit 255 from the recorded file Revision 4 00 LB 0175 Page 35 NeuroPort User s Manual o Syne with NeuroMotive Recording It will enable syncing with the NeuroMotive Video Recording and Tracking System if present o Split File Interval The recorded file will be split every so often indicated by this value Storage Needed at Current Configuration It indicates the amount of storage needed for recording using current settings Signal to Noise Ratio Signal to Noise Ratio SNR displays the amplitude of the channel and noise in It also displays the signal to noise ratio so that the quality of the signal can be monitored Readings can be saved as a tab delimited text file and opened in Excel or another program for future analysis The SNR for elec11 in the example below is 9 50 meaning that the signal is 9 5 times bigger than the noise Signal to Noise Ratio File View Help GS Seconds N Signal Noise e
46. ose Channels button to select a group of channels to add or remove from the raster plot display To add all channels to the display click the Add All button The Clear All button will remove all channels from the raster display o Open Selected Channels After selecting a channel or group of channels to display in Neural Channel click this button to open the selected channels Single Neural Channel This app displays the activity of a single channel It is also where units can be defined for the manual spike sorting algorithms Revision 4 00 LB 0175 Page 31 NeuroPort User s Manual Figure 38 Single Neural Channel Channel ID From the drop down box change the channel displayed Auto Toggles thresholding between adaptive threshold and a user defined fixed value Properties Display and change the settings of the current channel Spike Display The signal is displayed as time stamped events Use the vertical slide button at the right of the screen to set the voltage range The default time scale of the spike display window is 1 6 ms 48 samples spike width The time scale spike width samples can be defined in the Global Settings section in the Hardware Configuration module in a range from 1 0 ms to 4 3 ms 30 samples to 128 samples o Clear This button clears the spike display until new spikes are triggered Specify in the drop down box how many events will be displayed before clearing Spike Count Drop Menu Specify
47. ough the box in Spike Panel 9 Click File Save Values to save the settings to a data in a text file on the hard drive 10 Click File Exit to quit Impedance testing Auto Impedance will check the switch 11 A dialog will be displayed instructing you to turn the Impedance switch to the Normal position When the switch has been toggled to the Normal position Auto Impedance restores original channel settings and will close Do not leave the impedance switch in the Impedance position when not in the Auto Impedance feature If the switch is accidentally switched to the Impedance position when not testing significant noise will be present invalidating the recorded data Crosstalk If you suspect that you may be looking at the same neural activity on more than one channel the Crosstalk Diagnostic module will compare each channel for cross correlation The Crosstalk Diagnostic Module will display each channel as green yellow or red Revision 4 00 LB 0175 Page 37 NeuroPort User s Manual T maw Figure 45 Crosstalk O Green indicates that no cross correlation to any other channel was found o Yellow indicates that possible cross correlation is found with another marked yellow channel but not exact cross correlation It is suggested that the user closely compares any channels that turn yellow to determine if it is possible crosstalk o Red indicates that a high level of cross correlation is found with other red
48. ounting the number of spikes that occur over a fixed time window 20ms Smoothing adds steps between events and non events both between channels and over time on a channel smoothing out the event horizon that would otherwise be too abrupt a change and could possibly cause signals to be missed by the observer peer B Activity Map 0 9 18 0 Maimun Scale Range Color Scale spkes sec 133 A v Time Constant e 046 TIT Esas Bem Maglie path and name Load C ers tpi Document Code and LLT Araya Cerebus DelutMaplid Maple descnption Figure 39 Activity Monitor Pause When checked the display will pause The activity map will resume when the box is clicked again to clear Reset Button Press to restart the display of the firing rates Maximum Scale Range Allows the user to set the maximum value of the Color Scale Scale Eccentricity This setting allows the user to set how eccentric and sensitive the colors are to the firing rates time specified in seconds is how long each electrode area color will retain prior to returning to blue map file can be loaded to change the layout of the grid File Storage Use the File Storage Interface screen to record data The user can enter patient information and select a path for the files that are created when the session is initiated The NeuroPort Software assigns a numeric name to
49. packets are being received from the NeuroPort system If the PC is not receiving packets from the NeuroPort system see troubleshooting section 0 above 20 Verify that the fiber optic data link between the amplifier and the NSP is plugged in and that the green light next to the fiber optic data port on the NSP is illuminated If the light is red there is no fiber optic link present Check the LED next to the fiber optic port on the back of the amplifier to verify that it has power If the LED is not illuminated check the power supply and verify that it is turned on and not in standby mode Are the cables securely plugged in Revision 4 00 LB 0175 Page44
50. ptic cable An LED to the right of the connector turns green when a link is established and turns red when the link is broken 11 Synchronization Port This DB9 port is located in the back of the NSP and it is used in the synchronization of two NSPs The synchronization occurs automatically at startup if the sync cable Blackrock Part 5584 is properly connected between two NSPs Some models may not have this port To upgrade your system to add synchronization capability please contact Blackrock Microsystems support 10 Connectors Cables etc Headstage Requirements The high input impedance and low bias current of the front end inputs make it possible to connect microelectrodes with 1 or lower impedance values directly to the Front End Amplifier inputs without the need for a headstage This configuration has the advantage of avoiding noise added to the signals by the headstage but it makes the application more susceptible to environmental and power line noise To minimize environmental noise it is preferable to keep direct electrode connections shorter than 20 cm 8 inches For longer connections HSF 32 headstage units from Blackrock Microsystems are recommended These units allow microelectrodes with impedances up to 5 MQ and cables up to 1 meter 3 5 feet in length ICS Connectors The HSF 32 is built to work with Samtec www samtec com connectors and it is designed to mate with the ICS family of array holders from Blackrock M
51. rface 1 Gigabit Ethernet PC Software Interface Windows XP Pro External Power Supply Five channel with monitoring sequencing and emergency shutdown control Input 120 or 240 VAC 50 60Hz Outputs 5 0V 500mA analog 5 0 V 500 mA analog 33 3 V 300 mA quiet Digital 33 3 V 500 mA digital 45 0 V 300 mA digital Power Supply Standard 3 pin PC power connector accepting 110 240 VAC 50 60 Hz Revision 4 00 LB 0175 Page 41 NeuroPort User s Manual Warranty Blackrock Microsystems Blackrock warrants its products are free from defects in materials and manufacturing for a period of one year from the date of shipment At its option Blackrock will repair or replace any product that does not comply with this warranty This warranty is voided by 1 any modification or attempted modification to the product done by anyone other than an authorized Blackrock employee 2 any abuse negligent handling or misapplication of the product or 3 any sale or other transfer of the product by the original purchaser EXCEPT FOR THE WARRANTY SET FORTH IN THE PRECEDING PARAGRAPH Blackrock PROVIDES NO WARRANTIES OF ANY KIND EITHER EXPRESS OR IMPLIED BY FACT OR LAW AND HEREBY DISCLAIMS ALL OTHER WARRANTIES INCLUDING WITHOUT LIMITATION THE IMPLIED WARRANTIES OF MERCHANTABILITY FITNESS FOR A PARTICULAR PURPOSE AND NON INFRINGEMENT OF THIRD PARTY PATENT OR OTHER INTELLECTUAL PROPERTY RI
52. sholding Spike Sorting Adaptive Filtering N TRODE GROUPS SPIKE PANEL RASTER PLOT SINGLE NEURAL CHANNEL ANALYSIS SOFTWARE Activity MAP FILE STORAGE SIGNAL TO NOISE RATIO NEURAL MODULATION IMPEDANCE TESTER CROSSTALK N TRODE OTHER UTILITIES CENTRAL PLAY CENTRAL SIM N PLAY SERVER HARDWARE SPECIFICATIONS WARRANTY RETURN MERCHANDISE AUTHORIZATION RMA TROUBLESHOOTING EXTRANEOUS NOISE WINDOWS COMPATIBILITY ISSUES No POWER TO NEUROPORT SYSTEM LOST PACKETS ERROR NOTIFICATION NO NEURAL ACTIVITY ON ALL CHANNELS Revision 4 00 LB 0175 Page2 NeuroPort User s Manual Table of Figures FIGURE 1 SYSTEM OVERVIEW FIGURE 2 FRONT END AMPLIFIER FIGURE 3 FRONT END AMPLIFIER POWER SUPPLY FIGURE 4 NSP FRONT FIGURE 5 NSP BACK FIGURE 6 DIGITAL IN PIN DIAGRAM FIGURE 7 SERIAL I O PIN DIAGRAM FIGURE 8 HEADSTAGE ASSEMBLY AND CONNECTIONS FIGURE 9 FRONT END AMPLIFIER INPUT SCHEMATIC FIGURE 10 FIBER OPTIC CABLE FIGURE 11 CROSSOVER CABLE FIGURE 12 AMPLIFIER POWER SUPPLY CABLE FIGURE 13 NEURAL SIGNAL SIMULATOR FIGURE 14 1 GBPS ETHERNET CARD FIGURE 15 NETWORK CONNECTIONS WINDOW FIGURE 16 LOCAL AREA CONNECTIONS PROPERTIES FIGURE 17 IPV4 PROPERTIES FIGURE 18 CENTRAL MAIN APPLICATION FIGURE 19 THRESHOLDING WINDOW FIGURE 20
53. sify up to five different signals per channel each selected signal receives a separate color An ISI Histogram is also displayed for each classified spike Hoops Spike Sorting If Hoops spike sorting is selected you can click on Define not shown above next to each unit window box A color box will appear in the spike window Drag the box to place a hoop anywhere on the screen so that spikes go through that hoop Any spike going through the hoop will be classified as that unit Multiple hoops can be placed and only spikes that go through both hoops will be classified A user can sort an individual channel by clicking on the Pattern Click the Define button next to the classified spike to sort that unit Hold the Ctrl key and draw around the cluster An ellipsoid will be created which is used to define a unit You may also change the size of the ellipsoid by pressing R then moving the handle Revision 4 00 LB 0175 Page 32 NeuroPort User s Manual Unit Mapping Another override option can be performed by clicking the unit mapping button Rules can be remapped combined or classified as noise Revision 4 00 LB 0175 Page 33 NeuroPort User s Manual Analysis Software Activity Map This app gives the user an overview of the firing rates of each electrode spatially organized over time A map file can be loaded to change the electrode arrangement The spiking activity of each channel is binned and smoothed Binning is c
54. supply and turn it back on to reset the error n Figure 3 Front End Amplifier Power Supply Revision 4 00 LB 0175 Page 10 NeuroPort User s Manual The On Off switch is located on the back of the unit Once it is switched on the power supply will start a power up sequence of analog and digital supplies ending with the green ON LED illuminating When the power switch is turned off this sequence is reversed and the output of the amplifier will be tied to ground Neural Signal Processor NSP The NSP performs final processing and on line analysis of the neural signals and then transmits this data to the connected host PC via an Ethernet link ral Processor e Ej D d Figure 4 NSP Front Figure 5 NSP Back 1 Power switch It is used to turn the NSP on and off The LED above the switch will illuminate when the unit is on 2 LCD Display It displays the current operating status of the unit The statuses include Initializing NSP Startup NSP Running and Synchronized 3 Analog Inputs Analog experiment information can be recorded through 16 analog input BNC ports The analog source may range from 45 0 V and should come from a source impedance of less than 100 Q The coupling of each input channel can be manually selected in the software By default channels 1 to 8 are AC coupled and channels 9 16 DC coupled 4 Digital Input Digital signals may be
55. the channel settings made in Hardware Configuration Save Sorting Rules Saves spike sorting details Save PCA Basis Saves PCA basis vectors Close Applications Closes Central and all associated applications It leaves the NSP running Hardware Standby and Close It causes the system to stop gathering data File storage can be stopped prior to NSP going into Standby mode The NSP must be in Standby mode to update the firmware Hardware Shutdown and Close Closes Central and all its associated applications and stops the NSP Manually turn off the NSP power switch The Tools menu contains functions regarding thresholding spike sorting and general application settings Thresholding Set the manual threshold level for all channels by a RMS multiplier or by a fixed voltage value Revision 4 00 LB 0175 Page 19 NeuroPort User s Manual Figure 19 Thresholding Window o Options See below o Lock Unit Statistics The statistics used for the automatic spike sorting algorithm are adaptive over time Selecting this disables the adaptive part of the algorithm o Rebuild Spike Sorting Model This option restarts the automatic spike sorting function Options Set to various general options for the Central app EL k Allow multiple instances of 7 Hardware Configuration T Spike Panel Window EJ Raster Plot Window Log Rules Do Not Log Sort Changes Log Sorting Changes on Record
56. ths from fixed transmitters such as base stations for radio cellular cordless telephones and land mobile radios amateur radio AM and FM radio broadcast and TV broadcast cannot be predicted theoretically with accuracy To assess the electromagnetic environment due to fixed RF transmitters an electromagnetic site survey should be considered If the measured field strength in the location in which the NeuroPort system is used exceeds the applicable RF compliance level above the NeuroPort system should be observed to verify normal operation If abnormal performance is observed additional measures may be necessary such as reorienting or relocating the NeuroPort system Over the frequency range 150 kHz to 80 MHz field strengths should be less than 3V m Revision 4 00 LB 0175 Page 7 NeuroPort User s Manual Table 206 Recommended separation distances between portable and mobile RF communications equipment and the EQUIPMENT or SYSTEM for EQUIPMENT or SYSTEMS that are not LIFE SUPPORTING refer to 60601 1 2 the NeuroPort amp system Recommended separation distances between portable and mobile RF communications equipment and communications equipment The NeuroPort system is intended for use in an electromagnetic environment in which radiated RF disturbances are controlled The customer or the user of the NeuroPort system can help prevent electromagnetic interference by maintai
57. to detect microvolt level signals When the simulator is attached to the system touching or just holding your hand near the ribbon cables will cause noise on Spike Panel and Raster Plot This is not a fault but an indication that the location and arrangement of the connecting cables is critical to optimal performance Make sure that the cables connecting the amplifier to the NSP are kept away from the ribbon cables that input signals to the amplifier to avoid feedback problems and cross talk between channels The simulator is an excellent tool to detect subtle problems in the system setup Computer monitors can also be a major source of noise and should be kept away from the instrumentation field Windows Compatibility Issues On certain PCs running the Windows operating system some of the software modules or executable files rarely experience graphical issues If this happens set the compatibility mode in the file properties of the software to correct the issue Open the Release folder that NeuroPort installed on your PC then right click the module executable file Select the properties option in the drop down menu From the file properties screen click the Compatibility tab at the top of the window then click the box in the Compatibility mode section to run the program in compatibility mode for Windows 2000 NouroPort Windows Suite Properties 7 General Version Compalibily Secuily Summan Add C Pro
58. utions Federal law USA restricts this device to sale or the order of a physician Follow the restrictions of use for third party electrodes or arrays Third party recording systems connecting to the NeuroPort System and components must be electrically isolated for patient safety Revision 4 00 LB 0175 Page5 NeuroPort User s Manual Specifications Model Name NeuroPort Bio potential Signal Processing System Power Requirements 120 240 VAC 60 Hz 8 0 Amps maximum load UPS backup power available for up to 5 minutes Serviceable Fuses 5 x 20mm 250V 1 6A Slow Blow Compliance Standards IEC 60601 1 IEC 60601 1 2 IEC 60601 2 26 CSA listed Type of Protection Class Amplifier Power Supply Degree of Protection Type CF Applied Part Amplifier and Patient Cable Assembly Mode of Operation Continuous Water Ingress Protection Ordinary Equipment not fluid resistant IP20 Operating Environment 10 C to 40 C 5 to 95 R H non condensing Storage Environment 20 C to 50 C 5 to 100 R H non condensing Revision 4 00 LB 0175 Page 6 NeuroPort User s Manual Table 204 Guidance and manufacturer s declaration electromagnetic immunity for all EQUIPMENT and SYSTEMS that are not LIFE SUPPORTING refer to 60601 1 2 Guidance and manufacturer s declaration electromagnetic immunity The NeuroPort System is intended
59. ystem reset 14 Contact Product Support at 866 806 3691 or 801 582 5533 for assistance Lost Packets Error Notification If an error message is displayed that network packets were lost during analysis or recording or that no packets are being received when running Central verify the following 15 Check the Ethernet Cat5 e cable and verify that it is secure in the 1 Gigabit Ethernet card in the PC and the RJ 45 port on the back of the NSP labeled To PC 16 Look at the Central Control module and verify that the PC system load is not too high If the PC system load is too high adjust the thresholds on all channels to lower the load on the PC or lower the sample rate for continuous waveforms if they are enabled Are any other programs running on the Host PC If so close any open programs that do not need to be running while using the NeuroPort system 17 Does the Host PC meet the minimum specifications for the NeuroPort system Contact product support for the PC specifications at 801 582 5533 or 866 806 3692 18 Isa firewall enabled on the Host PC Some issues with firewalls have been known to block data packets between the NeuroPort system and the PC Disable the firewall then reboot the Host PC No Neural Activity on all Channels If the NSP is turned on and running but there is no apparent activity on any of the channels from an implant or from the simulator verify the following 19 Open Central exe and verify that
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