User Guide
Contents
1. 4 Return instruments using 3 day air shipping or better do not ship via ground Return Policy for Instruments with Anti fouling Treatment WET Labs cannot accept instruments for servicing or repair that are treated with anti fouling compound s This includes but is not limited to tri butyl tin TBT marine anti fouling paint ablative coatings etc Please ensure any anti fouling treatment has been removed prior to returning instruments to WET Labs for service or repair ECO FLNTU User s Guide flntu Revision Z 13 May 2009 WET Labs Table of Contents 1 SPECNICAUONS surreal 1 2 ECO Meter Components 2202222022020snenn nen nnenn nennen nenne nennen 3 M AOI COIS II E 3 22 Diva OS rias 4 2 3 Optional Equipment sssssssssssssseseennnnnnnnnssnnennnnnnnnnnnnnnnnnnnnsnnnnnnnnsnnnnnnnnsnnnsnnnnnnnnnnnnnnnn 4 Theory of Operation u24002s02n0nnnnen one nen nenenne nennen nenne nenne T Instrument Operation coococconoccocionoccononnononnonoononconennorennenconennonens 8 A InsO ocx Ko enn ee eee ee eee 8 4 2 Operating the Sensor tor Data Oypul uuu nennen 8 wo DON A Innen ee see ee ee see eee 9 LEER DO ee ee ee S 9 25 Upke pan Mantendaco ta 10 9 FLNTUB Using Internal Batteries 13 5l Removins End Planse and B lle aan 13 5 2 Replacing End Flange and Batteries anna de a a UMME 14 sS Check ins Yen Pie nee een 15 6 Dala ANAIS Sama eee ean2. dark counts the measured signal output in VDC of meter in clean water with black tape over the detector Scale factor multiplier in NTU volts 6 3 Digital Response Digital data is processed in a similar fashion to analog data Scaling is linear and obtaining a calibrated output simply involves subtracting a digital offset value from output when measuring a sample of interest and multiplying the difference by the instrument scaling factor Chlorophyll Chl sample Coutput Cac Scale Factor where Chl sample concentration of a chlorophyll sample of interest ug l Coutput output when measuring a sample of interest counts Cac dark counts the measured signal output of meter in clean water with black tape over the detector Scale factor multiplier in ug l counts NTU NTU sampte NTU output Cac Scale Factor where NTU sample concentration of NTU solution NTU output output when measuring a sample of interest counts Cac dark counts the measured signal output of meter in clean water with black tape over the detector Scale factor multiplier in NTU counts ECO FLNTU User s Guide flntu Revision Z 13 May 2009 17 T WET Labs Characterization and Testing ECO FLNTU is configured for a chlorophyll measurement range of 0 04 50 ug l The turbidity sensor s measurement range is 0 25 NTU Gain selection is done at WET Labs by setting several gain settings inside the instrume
3. making sure the cut out accommodates the vent plug screw 8 Push the end flange all the way on to the pressure housing making sure no wires are pinched Be sure the vent plug does not pop up If it does you ll need to re position the foam spacer 9 Re insert the monofilament 5 3 Checking Vent Plug If there is fouling on the vent plug it should be cleaned and the two 010 O rings replaced Otherwise this mechanism should be maintenance free WARNING The pressure housing is made of plastic material that scratches easily Do not let the screwdriver slip and scratch the can when removing or replacing the vent plug Use a toothpick something softer than the plastic to remove the O rings from the vent plug 1 Pull vent plug out about half way hold plug while unscrewing the truss screw When screw is removed pull vent plug from end flange 2 Pinch bottom O ring around vent plug to form a small gap you can work a toothpick into Use the toothpick to help roll the bottom O ring off the plug 3 Perform the same procedure with the top O ring 4 Clean the vent plug and vent plug hole using a dry lint free tissue or cotton swab 5 Lightly coat two undamaged or new O rings with silicon grease Install the top O ring nearest to large end of plug first then the bottom one 6 Insert vent plug into its hole in the end flange and hold it while inserting the truss screw Rotate the vent plug to begin tightening the screw
4. 0 01 ug l 0 01 50 ug l 99 R Wavelength excitation Wavelength emission Sensitivity per count Range typical Linearity both signals ECO FLNTU User s Guide flntu Revision Z 13 May 2009 1 WET Labs FLNTU RT Provides analog or RS 232 serial output with 4 000 count range This unit operates continuously when powered Available with a 6 000 m depth rating FLNTU Provides the capabilities of the FLNTU RT with periodic sampling Available with a 6 000 m depth rating FLNTUS Provides the capabilities of the FLNTU with an integrated anti fouling bio wiper and copper faceplate FLNTUB Provides the capabilities of the FLNTU with internal batteries for autonomous operation FLNTUSB Provides the capabilities of the FLNTUS with internal batteries for autonomous operation 2 ECO FLNTU User s Guide flntu Revision Z 13 May 2009 WET Labs 2 ECO Meter Components The following subsections describe the bulkhead connectors items delivered with the ECO sensor and optionally available equipment 2 1 Connectors ECO Series meters use a s x pin bulkhead connector Pin Function MCBH 6 MP RS 232 TX 6 FLamalogout Input power of 7 15 VDC is applied to V in pin 4 The power supply current returns through the common ground pin pin 1 The input power signal has a bi directional filter This prevents external power supply noise from entering into ECO FLNTU and also prevents internally generated
5. 16 MEC ICM Ud mE 16 C2 AA taddier tiat seveendsecebucntpuhuadesen EE tum ME 16 0 3 Disttal RESPONSE ia 17 Characterization and Testing oooccocccoccocncncocancnonnnconanenannnnonas 18 Terminal Communications ccoccocnccccnconcnnoncononcnnnncnconnnnanonnonnss 19 Sl Im enaco DP CAI ONG ria 19 MEE Coma en 19 9 Device and Output Files 02200240240020 Bene nnn nennen nnn nennen 20 ZA EE odis co eines nn A ENTE HESE E EEEO NRE NS 20 92 Colima Count Speer MCU OM nennen 20 9 3 Column Description ee eeeeeeesssseeeceeeecceeeeeeeaaaaaseseseseeeeeeeeeeeeeeeseseeaauassesesseseeeeeeeeeeeeees 20 94 Sample Device PUES esas 21 9 5 Sample Output FllesS ooooooonononnncnoncconnnonononononnnononononononnnnnnnnnnnnnnnnnnnnnnnnonnnnnnnnnnnnnnnnnos 21 Appendix A Mounting Bracket Drawing occoccoccoccoccccncnncnncancnnonnnnnnnos 22 ECO FLNTU User s Guide flntu Revision Z 13 May 2009 wET Labs 1 Specifications Model FLNTU RT D FLNTU FLNTUB FLNTUS FLNTUSB 600m 13k gt J gt 600m og Acetal copolymer 600m mem o Environmental 5090 Oi sv Current seep 49A Data memory samples O o zt O 3 D gt o S Q bio wiper Bio wiper cycle 140 mA O Lo O aL O D 0 O D Q D 470 nm 695 nm
6. Finish tightening using a screwdriver being careful not to over tighten truss screw Note A portion of the truss screw head has been removed to allow for venting in case of pressure buildup ECO FLNTU User s Guide flntu Revision Z 13 May 2009 15 6 WET Labs Data Analysis Data from the ECO fluorometer and turbidity sensor whether digital or analog represents raw output from the sensor Applying linear scaling constants this data can be expressed in meaningful forms of chlorophyll fluorescence and NTUs 16 6 1 Scale Factor The scale factor 1s factory calculated by obtaining a consistent output of a solution with a known concentration then subtracting the meter s dark counts The scale factor dark counts and other characterization values are given on the instrument s characterization sheet Chlorophyll For chlorophyll WET Labs uses the chlorophyll equivalent concentration CEC as the signal output using a fluorescent proxy approximately equal to 25 ug l of a Thalassiosira weissflogii phytoplankton culture Scale Factor 25 ug l Chl Equivalent Concentration dark counts For example 25 3198 71 0 0080 NTU Scale Factor xx meter output dark counts where xx is the value of a Formazin concentration For example 12 2 2011 50 0 0062 The scale factor is then applied to the output signal to provide the direct conversion of the output signal to chlorophyll concentration WET La
7. Memory On 5 Ifthe meter does not light after performing step 3 check the battery Replace if necessary perform steps 2 and 3 to verify communication If it still does not light contact WET Labs Refer to the ECOView User s Guide for details about using the software 4 3 Bio wiper Operation The ECO FLNTUS and FLNTUSB are provided with an anti fouling bio wiper and faceplate that extend the possible deployment duration by retarding biological growth on the instrument s optical surface The Bio wiper covers the optical surface 1 while the instrument s n sleep mode 2 when t has completed the number of samples requested When the meter wakes up the optical surface is exposed by the Bio wiper s counter clockwise rotation If power is shut off in mid cycle the Bio wiper will reinitialize to the beginning of the user selected settings when power 1s applied again 4 4 Deployment WARNING FLNTUB and FLNTUSB Always check vent seal plug for full insertion immediately prior to deployment Caution The FLNTU should be mounted so the LED source will not see any part of a cage or deployment hardware This will compromise the sensor s output Once power is supplied to the ECO meter the unit 1s ready for submersion and subsequent measurements Some consideration should be given to the package orientation Do not face the sensor directly into the sun or other bright lights For best output signal integrit
8. These samples are collected at one second intervals for one minute A standard deviation is then performed on the 60 samples and the result is the published noise on the calibration form The calculated noise must be below 2 counts Voltage and Current Range Verification To verify the ECO operates over the entire specified voltage range 7 15 V a voltage test is performed at 7 and 15V and the current draw and operation is observed The current must remain constant at both 7 and 15V ECO FLNTU User s Guide flntu Revision Z 13 May 2009 wET Labs 8 Terminal Communications As an alternative to the ECOView host software ECO sensors can be controlled from a terminal emulator or customer supplied interface software This section outlines hardware requirements and low level interface commands for this type of operation 8 1 Interface Specifications e baud rate 19200 e data bits 8 e parity none e stop bits 1 e flow control none 8 2 Command List Parameters passed none Stops data collection allows user to input setup parameters Note that if the meter is in a sleep state the power must be turned off for a minute then powered on while the key is held down for several seconds If this does not wake the meter refer to the ECOView user s guide Operation Tip to wake a meter in a low power sleep state to enable inputting setup parameters Sclk get mone Reads data out of Atmel memory chip Prints etx
9. be conclusively deemed to include a covenant by the customer to defend and hold WET Labs Inc harmless from all product liability claims arising from the use and servicing of this equipment Flooded instruments will be covered by WET Labs Inc warranties at the discretion of WET Labs Inc l Make sure the instrument 1s thoroughly dry 2 Remove the dummy plugs 3 With connector end flange pointed downwards away from face release seal from vent plug 4 Remove moisture from vent plug area 5 Using needle nose pliers remove filament from end flange 6 Lift flange from pressure housing until seal 1s broken The jacking screws can be used to push the flange from the pressure housing and then can be removed or left in the end flange ECO FLNTU User s Guide flntu Revision Z 13 May 2009 13 14 10 11 12 WET Labs Remove any excess moisture from flange can seal area Work end flange out of pressure housing and remove any residual moisture Remove the gray foam spacer and the neoprene insulator The battery pack is connected to the processor boards by a six pin Molex connector do NOT pull too hard or far on the battery pack or it will come unplugged and the unit will need to be returned to WET Labs Gently pull the white cord at the loop to remove the battery pack from the pressure housing Remove the black plastic protectors from the ends of the long screws securing the batteries Loosen and remove the screw
10. slide on easily insert the screw into the expander hole turning slowly until the bio wiper slides easily onto the shaft 10 Rotate the bio wiper into the closed position 11 Set the gap between the bio wiper M and the instrument face to 0 03 in 0 8 mm An improperly set gap will either fail to clean the face or cause the motor to draw excessive current To gauge 0 03 in fold a piece of paper in 03 half then in half again then fold a third time creasing the edges It s now 8 sheets and about 0 03 in thick ECO FLNTU User s Guide fintu Revision Z 13 May 2009 11 Not enough flex Proper flex Too much flex Wiper may not be Wiper maintains contact with Wiper may cause too much effective instrument face and friction using excessive power optical window 12 Use the 3 32 in hex key to tighten the screw to finger tight then snug an additional quarter turn 13 Run the instrument to verify operation The bio wiper must rotate 180 degrees to clear the optics before sampling and 180 degrees to cover the optics after sampling 14 If the wiper needs adjusting loosen the screw make any necessary adjustments and repeat steps 9 through 13 to ensure the wiper is performing properly ECO FLNTU User s Guide flntu Revision Z 13 May 2009 wET Labs 5 FLNTUB Using Internal Batteries ECO sensors powered with internal batteries can either run directly from the internal batteries or can ope
11. when completed 2 Ahr format time hhmmss Time interval between packets in a set rints the menu including time and date P ET Spkt single number 0 to 65535 Number of individual measurements in each packet S rc on or 0 off Enables or disables recording data to Atmel memory chip E N ECO FLNTU User s Guide flntu Revision Z 13 May 2009 19 9 WET Labs Device and Output Files Each meter 1s shipped with a CD containing the meter specific device file a sample output file characterization information and the applicable user s guides The ECOView host program requires a device file to provide engineering unit outputs for any of its measurements Except for the first line in the device file all lines of information in the device file that do not conform to one of the descriptor headers will be ignored Every ECOView device file has three required elements Plot Header Column Count Specification and Column Description 20 9 1 Plot Header The first line in the device file is used as the plot header for the ECOView Plots 9 2 Column Count Specification The Column Count Specification identifies how many columns of data to expect It follows the format Column n The Column Count Specification must be present before any of the Column Descriptions are listed 9 3 Column Description Every column in the ECO meter s output must have a corresponding Column Description in the device file The followi
12. EF 5 NTU 6 N U g 0 0063 83 0 9 5 Sample Output Files The reference column is unused by both firmware versions but in 3 01 the emission wavelength of the chlorophyll signal and the scattering wavelength are displayed 9 5 1 Firmware Versions 3 01 and Higher Date Time A CHL A NTU Thermistor MM DD Y Y HH MM SS Signal Signal 08 03 07 11 22 51 695 49 700 69 536 08 03 07 11 22 53 695 48 700 68 536 08 03 07 11 22 54 695 49 700 68 536 08 03 07 11 22 56 695 49 700 67 536 08 03 07 11 22 58 695 47 700 69 536 08 03 07 11 23 00 695 48 700 68 536 9 5 2 Firmware Versions Prior to 3 01 Date Time Chl Ref Chl NTU NTU Thermistor MM DD Y Y HH MM SS Signal Ref Signal 05 01 03 10 06 51 1817 49 1583 69 536 05 01 03 10 06 53 1818 48 1583 68 536 05 01 03 10 06 54 1818 49 1583 68 536 05 01 03 10 06 56 1818 49 1584 67 536 05 01 03 10 06 58 1819 47 1584 69 536 05 01 03 10 07 00 1819 48 1583 68 536 ECO FLNTU User s Guide flntu Revision Z 13 May 2009 21 WET Labs Appendix A Mounting Bracket Drawing 620 Applegate St REVISIONS Philomath OR 97370 REV DATE DESCRIPTION BY W ET La bs n C Ph 541 929 5650 a 3 9 04 CHANGETO ROUNDED BRACKET ARD e Fax 541 929 5277 1 4 20 x 1 socket head cap screws 2 washers and nylon insert nuts Use 3 16 Allen wrenc
13. Section 6 DCR 477 Wetzel O 1 23 06 Add deep specs DCR 483 D Hankins H Van Zee P 3 3 06 Add copper faceplate DCR 490 ECN 217 H VanZee l Walsh IEA 5 31 06 maintenance recommendation DCR 498 S Proctor NP Cleaning and maintenance of modified bio wiper ECN PA 6 28 06 230 DCR 502 A Derr H Van Zee ME Change length of securing screw on bio wiper ECN not nov S 7 27 06 assigned DCR 504 J da Cunha H Van Zee 9 28 06 Update optical and electrical specifications DCR 507 Correct Pressure Sensor and Thermistor output equations U 11 1 06 DCR 509 M Johnson Change unused Reference column in output to N U Correct battery units to 300 m depth rating Remove reference to refilling silicone oil in pressure sensors ECN V 8 28 07 M Johnson H Van Zee Use one instead of two LEDs ECN 264 delete reference to refilling pressure sensor update shipping requirements W 9 11 07 DCR 531 M Johnson H Van Zee eee Change dark counts derivation to reflect current production X 7 10 08 methodology DCR 600 H Van Zee Lune Update specs correct Molex connector information DCR PPM Y 11 12 08 621 S Lohse 5 13 09 Correct electronic stability test parameters DCR 669 o ECO FLNTU User s Guide flntu Revision Z 13 May 2009
14. WET Labs Combination Fluorometer and Turbidity Sensor ECO FLNTU User s Guide The user s guide is an evolving document If you find sections that are unclear or missing information please let us know Please check our website periodically for updates WET Labs Inc P O Box 518 Philomath OR 97370 541 929 5650 fax 541 929 5277 www wetlabs com ECO FLNTU User s Guide flntu Revision Z 13 May 2009 WET Labs ECO Sensor Warranty This unit is guaranteed against defects in materials and workmanship for one year from the original date of purchase Warranty is void if the factory determines the unit was subjected to abuse or neglect beyond the normal wear and tear of field deployment or in the event the pressure housing has been opened by the customer To return the instrument contact WET Labs for a Return Merchandise Authorization RMA and ship in the original container WET Labs is not responsible for damage to instruments during the return shipment to the factory WET Labs will supply all replacement parts and labor and pay for return via 3 day air shipping in honoring this warranty Shipping Requirements 1 Please retain the original Pelican shipping case It meets stringent shipping and insurance requirements and protects your meter 2 Service and repair work cannot be guaranteed unless the meter is shipped in its original case 3 Clearly mark the RMA number on the outside of your case and on all packing lists
15. bs supplies a scale factor on the instrument specific calibration sheet that ships with each meter While this constant can be used to obtain approximate values field calibration 1s highly recommended 6 2 Analog Response The ECO FLNTU response 1s linear over the measurement range provided Because of the varied environments in which each user will work it is important to do calibrations using similar seawater as you expect to encounter in situ Refer to the characterization section for further details This will provide an accurate blank equivalent phytoplankton types and similar physiological conditions for calculating the scale factor thereby providing an accurate and meaningful calibration Once a zero point has been determined and a scale factor established the conversion of DC volts to chlorophyll concentration and NTUS is straightforward using the equations ECO FLNTU User s Guide flntu Revision Z 13 May 2009 WET Labs Chl sample V output Vac Scale Factor where Chl sample concentration of a chlorophyll sample of interest ug l V output output when measuring a sample of interest VDC Vac dark counts the measured signal output in VDC of meter in clean water with black tape over the detector Scale factor multiplier in ug l volts NTU NTU sampie V output Vac Scale Factor where NTU Isample level of turbidity NTU V output output when measuring a sample of interest VDC Vac
16. ct anti fouling Bio wiper and copper faceplate for use in extended deployments This wiper can be manually controlled by a host controller package or can perform autonomously as part of a pre programmed sampling sequence upon instrument power up The rate of closure and opening is dependent upon both temperature and depth Refer to Section 4 5 1 for important details on the maintenance and cleaning of the Bio wiper and copper faceplate WARNING Do NOT rotate the Bio wiper manually This can damage the wiper motor and will void the warranty 2 3 4 Batteries ECO units with internal batteries are supplied with six 9 volt Lithium batteries as their power source They can use either standard alkaline cells for a total capacity of approximately 1000 mA hrs or for longer deployments LiMnO cells to achieve more than 2000 mA hrs of operational capacity Actual total usage time of the internal batteries 1s a function of several parameters These include nominal water temperature sequence timing sample periods and total deployment duration WARNING Be sure to keep the dummy plug on the FLNTUB and FLNTUSB when not in use For even greater deployment capability contact WET Labs for information on external battery packs 2 3 5 External Thermistor ECO meters are optionally equipped with an external thermistor The thermistor 1s calibrated at WET Labs and the calibration coefficients are supplied on the instrument s calibration shee
17. h and 7 16 10 24 x 1 2 socket head cap screws 4 Use 5 32 ARS wench Allen wrench Do not overtighten MCBH 6 M bulkhead connector 9 7 1 75 p quot oO 6 0 5 7 5 0 II Em 0248 BioWiper shown in the closed position BioWiper equipped instrument only Instrument without BioWiper TOLERANCES 3 Be ECO MOUNTING DETAILS XXX 0 01 X XXX X XXXX 0 005 SENE SIZE ENGR A A R DERR 220233 A SCALE DATE 1 0 SHEET 1 4 10 22 2002 10F 1 ECO FLNTU User s Guide flntu Revision V 7 March 2007 22 WET Labs Revision History Revision Date Revision Description A New document DCR 322 Correct syntax in 8 3 2 DCR 336 C Add Terminal Communications section DCR 337 Modify explanation for stop data collection command DCR 11 24 03 342 W Strubhar E 11 25 03 Update specifications DCR 338 Walsh O A O B L C GE A LI Tue Update bio wiper maintenance and column description for O F 2 17 04 device files DCR 367 A Derr I Walsh ESTER G mounting diagram DCR 381 A Derr D Whiteman H 5 6 29 04 Update specifications DCR 400 I Walsh ET nun itm HTH TOR gy J 429 Walsh Tanne SEER PITRE PIPER gy O K 10 14 04 DCR 433 Walsh ENFERMO NNNM L 7126 05 Dark Counts M Johnson H Van Zee R Watte C 3 10 04 H 10 04 Delete warning about pins 3 and 6 DCR 390 Walsh 9 28 04 M 12 7 05 Clarify
18. ion are configuration dependent These are noted where applicable within the manual 4 1 Initial Checkout Supplied from the factory ECOs are configured to begin continuously sampling upon power on Electrical checkout of ECO is straightforward using the optional test cable Connect the 6 socket connector on the test cable to the instrument to provide power to the LEDs and electronics see Section 1 for a diagram of the pin outs of ECO FLNTU Connect the battery leads on the test cable to the 9V battery supplied with the meter Light should emanate from the meter 4 1 1 Analog Option Connect a digital multimeter DMM to the auxiliary leg of the test cable the center of the RCA connector provides analog out signal and the outside provides ground Auxiliary chl 1s the fluorometer output Auxiliary NTU s the NTU output With the sensor face clean and dry the instrument should read approximately 0 050 0 095 VDC The analog signal will saturate at approximately 5 volts 4 2 Operating the Sensor for Data Output Note ECO scattering meters are sensitive to AC light Before making measurement turn AC lighting off l Connect the 6 socket connector to the instrument to provide power to the LEDs and electronics Connect the DB 9 connector to a computer with the ECOView host program installed on it WARNING Always use a regulated power supply to provide power to ECO sensors if not using the 9V battery provided with the test cab
19. le power spikes may damage the meter 2 Start ECOView Select the appropriate COM Port and Device File Supply power to the meter then click on the Start Data button Output will appear in the Raw Data window Test the instrument s signal using the fluorescent stick ECO is sensitive to room lighting for best results perform test in ambient light only turn off AC lighting Remove the protective cover Hold the fluorescent stick 1 4 cm above the optical paths in an orientation that maximizes exposure of the stick Parallel with the beams not intersecting them The signal will increase toward saturation maximum value on characterization sheet When applying power to sensors with a Bio wiper M it will open and depending on the settings operate until you select Stop Data in ECOView or input the next command 8 ECO FLNTU User s Guide flntu Revision Z 13 May 2009 WET Labs 3 Ifthe sensor completes the requested samples this is common for meters set up in moored applications it will go into sleep mode and the meter will not light when power is cycled To wake the meter click Stop Data five times at the rate of two times per second immediately upon applying power This interrupts the sensor returning it to a ready state awaiting commands 4 Check the settings for the ECO and change if necessary ECOView factory settings for continuous operation Set Number of Samples 0 Set Number of Cycles 0 Internal
20. mping hole and screw it into the releasing hole tightening it just enough to free the Bio wiper M from the shaft clamping screw hole releasing screw hole 3 Remove faceplate Use a small Phillips screwdriver to remove the screws that attach the plate to the optics head WARNING Be sure to retain and re use the factory installed screws as they are vented for pressure compensation 10 ECO FLNTU User s Guide flntu Revision Z 13 May 2009 WET Labs 4 Wash bio wiper and or copper faceplate with soapy water Rinse and dry thoroughly Note the condition of the copper on the instrument side of the wiper It 1s normal for copper to corrode and turn green especially after the instrument has been removed from the water This corrosion will slightly reduce the shutter s anti fouling ability the next time it 1s deployed 5 Buffeach with a pad of green Scotch Brite or similar until shiny 6 Clean the bio wiper shaft and the shaft hole using an isopropyl alcohol saturated cotton swab Allow to dry 7 Re install faceplate 8 Check the screw used to secure the bio wiper to the shaft a hex key must fit snugly into the screw socket If the socket 1s in any way compromised use a new screw 4 40 x 3 8 in 316 stainless steel treated with anti seize These are shipped as part of the meter s spare parts kit 9 Slide the bio wiper M over the shaft Be careful not to twist it on thus rotating the shaft If the wiper does not
21. ng notation is used in identifying the elements of each Column Description x the column number starting with 1 as the 1 column sc scale dc dark counts meter output in clean water with optics head taped mw wavelength measured by the sensor dw display wavelength color to plot scattering data in v measured volts dc Valid Column Descriptions are listed in the subsections below 9 3 1 Fluorescence Measurements CHL X SC dc IENGR X SC dc PHYCOERYTHRIN x SC dc URANINE X SC dc RHODAMINE X SC dc CDOM X SC dc 9 3 2 Turbidity Measurements NTU X SC dc 9 3 3 Miscellaneous Date X MMDD YY Time X HH MM SS N U X Not Used ECO FLNTU User s Guide flntu Revision Z 13 May 2009 WET Labs Scale offset and output are factory set engineering unit calculations the user can manually adjust Engineering units are displayed through ECOView n the Plot Data tab Parameters for changing the internally calculated engineering units iengrscale sc engroffset off iengrunits label where label is any continuous character string 9 4 Sample Device Files Below are sample device files for ECO FLNTU meters 9 4 1 Firmware Version 3 01 and Higher ECO FLNTUS 785 Created on 08 03 07 COLUMNS DATE TIME N U CHL N U NTU N U 0 0128 6250 0 0063 Sis 0 WO OF WNE I 9 4 2 Firmware Versions Prior to 3 01 ECO FLNTUS 045 Created on 07 03 03 COLUMNS 7 DATE 1 TIME 2 REF 3 CHL 4 0 0128 62 0 R
22. noise from coupling out on to the external power supply wire Data 1s sent out the serial output pin 2 1 1 ECO FLNTUB FLNTUSB Connectors FLNTUB and FLNTUSB units with internal batteries have a second bulkhead connector that comes with a jumper plug to supply power to the unit The pin functions for this connector are shown below SOCKET 1 PRESSURE PIN 3 HOLES FOR 4 40 JACK SCREW USED FOR REMOVING END FLANGE BULKHEAD CONNECTOR MCBH 3 F BULKHEAD CONNECTOR MCBH 6 M MATING IN LINE CONNECTOR MCIL 3 M MATING IN LINE CONNECTOR MCIL 6 F IN LINE LOCK COLLAR MCDLS F IN LINE LOCK COLLAR MCDLS F DUMMY PLUC MCDC 3 M DUMMY PLUG MCDC 6 F ECO FLNTUB and FLNTUSB connector schematic ECO FLNTU User s Guide flntu Revision Z 13 May 2009 3 2 2 WET Labs Pinout summary for ECO FLNTUB FLNTUSB Function Socket FLNTUB and FLNTUSB internal battery units are supplied with a jumper plug that provides power from the internal batteries for autonomous operation Delivered Items The standard ECO delivery consists of the following the instrument itself dummy plug with lock collar protective cover for optics this user s guide ECOView user s guide ECOView host program and device files on CD instrument specific calibration sheet Non battery units only stainless steel mounting bracket and hardware See Appendix A for details Internal battery units six 9 V Lithium batteries installed Spare Parts equipmen
23. nt and running a dilution series to determine the zero voltage offset and to ensure that the dynamic range covers the measurement range of interest The dilution series also establishes the linearity of the instrument s response As 1s the case with other fluorometers a detailed characterization must be done by the user to determine the actual zero point and scale factor for his her particular use The tests below ensure the meter s performance l 18 Dark counts The meter s baseline reading in the absence of source light is the dark count value This is determined by measuring the signal output of the meter in clean de ionized water with black tape over the detector Pressure To ensure the integrity of the housing and seals ECOs are subjected to a wet hyperbaric test before final testing The testing chamber applies a water pressure of at least 40 PSI Mechanical Stability Before final testing the ECO meters are subjected to a mechanical stability test This involves subjecting the unit to mild vibration and shock Proper instrument functionality is verified afterwards Electronic Stability This value is computed by collecting a sample once every second for twelve hours or more After the data 1s collected the standard deviation of this set s calculated and divided by the number of hours the test ran The stability value must be less than 2 0 counts hour Noise Noise is computed from a standard deviation over 60 samples
24. rate from power supplied by an external DC power supply 7 15 volts Internal to external source conversion is facilitated by a jumper plug that plugs into the unit s bulkhead connector When inserted the plug forms a connection from the battery to the electronics power supply By removing the plug the instrument can be powered and communicate via a test or deployment cable Setup conditions instrument checkout real time operation and data downloading are thus all achieved identically to the methods prescribed for the FLNTU and FLNTUS units 5 1 Removing End Flange and Batteries WARNING Changing the batteries will require opening the pressure housing of the ECO sensor Only people qualified to service underwater oceanographic instrumentation should perform this procedure If this procedure is performed improperly it could result in catastrophic instrument failure due to flooding or in personal injury or death due to abnormal internal pressure as a result of flooding WET Labs Inc disclaims all product liability from the use or servicing of this equipment WET Labs Inc has no way of controlling the use of this equipment or of choosing qualified personnel to operate it and therefore cannot take steps to comply with laws pertaining to product liability including laws that impose a duty to warn the user of any dangers involved with the operation and maintenance of this equipment Therefore acceptance of this equipment by the customer shall
25. rbidity at 700 nm within the same volume The fluorometer allows the user to monitor chlorophyll concentration by directly measuring the amount of chlorophyll a fluorescence emission from a given sample volume of water Chlorophyll when excited by the presence of an external light source absorbs light in certain regions of the visible spectrum and re emits a small portion of this light as fluorescence at longer wavelengths Two bright blue LEDs centered at 455 nm and modulated at 1 kHz provide the excitation source A blue interference filter 1s used to reject the small amount of red light emitted by the LEDs The blue light from the sources enters the water volume at an angle of approximately 55 60 degrees with respect to the end face of the unit Fluoresced light is received by a detector positioned where the acceptance angle forms a 140 degree intersection with the source beam A red interference filter is used to discriminate against the scattered blue excitation light The red fluorescence emitted is synchronously detected by a silicon photodiode Turbidity is measured simultaneously by detecting the scattered light from a 700 nm LED at 140 degrees to the same detector used for fluorescence The turbidity measurement is performed at the same 140 degree angle as the chlorophyll fluorescence ECO FLNTU User s Guide flntu Revision Z 13 May 2009 7 WET Labs 4 Instrument Operation Please note that certain aspects of instrument operat
26. s 3 16 1n slotted driver Replacing End Flange and Batteries Replace the batteries Re install the screws e Align the groove in each of the plates so the six wire extension bundle will fit in it along its length e Be careful not to cross thread into the bottom end plate nor to over tighten the screws e If they are too tight the fiber washers that act as separators between the batteries will flex e Make sure there are equal amounts of screw threads protruding from the bottom end plate when they are secure This will ensure the pack is straight and will fit into the pressure housing with no difficulty Re install the black plastic protective covers on the ends of the screws Remove and check the pressure housing O ring for nicks or tears Replace if necessary Before re installing apply a light coat of vacuum grease on the O ring Carefully replace the battery pack in the pressure housing Place the neoprene insulator on the battery assembly and lay the white cord on the top Plug in the three pin then the six pin Molex connectors Sensor operation can now be tested 1f desired Align the hole in the end flange NOT the jack screw holes with the white dowel pin While coiling the six wire bundle and making sure none are pinched between the end flange and the pressure housing position the flange on the housing Leave ECO FLNTU User s Guide flntu Revision Z 13 May 2009 WET Labs space to re insert the gray foam spacer
27. t dependent 2 3 Fluorescent stick for bench testing Bio wiper units one 3 32 in hex key for bio wiper removal Bio wiper units Three 4 40 x 3 8 in 316 stainless steel screws for securing bio wiper Internal battery units Two end flange O rings size 224 and two vent plug O rings size 010 Two jacking screws for connector flange removal One 3 32 1n hex key for jacking screws Power plug for autonomous operation Three pre cut segments 7 inches of 0 036 inch diameter monofilament for end flange Three pre cut segments 0 25 inches of 0 094 inch diameter white nylon bar stock for replacing the white plastic dowel pin O O O Qu uu Optional Equipment 2 3 1 Test Cable A test cable is optionally available with each unit This cable includes 1 An inline connector for providing power to the instrument from a user supplied 9V battery An auxiliary analog out connector A second auxiliary analog out connector for NTU only A DB 9 serial interface connector A six socket connector for providing power and signal to the instrument E ECO FLNTU User s Guide flntu Revision Z 13 May 2009 WET Labs 2 3 2 Copper Faceplate ECO meters are optionally equipped with copper faceplates to improve the meter s resistance to biofouling Refer to Section 4 5 1 for important details on maintenance and cleaning 2 3 3 Bio wiper and Copper Faceplate The FLNTUS and FLNTUSB are equipped with an integrated non conta
28. ts Thermistor output is in counts and can be converted into engineering units using the instrument s device file and ECOView software or the raw data can be converted in the user s software e g MATLAB or Excel using the calibration equation Temperature deg C Output Slope Intercept 2 3 6 Pressure Sensor ECO meters are optionally equipped with a strain gauge pressure sensor The pressure sensor is calibrated at WET Labs and the calibration coefficients are supplied on the instrument s calibration sheets Pressure sensor output is in counts and can be converted into engineering units using the instruments device file and ECOView software or the ECO FLNTU User s Guide flntu Revision Z 13 May 2009 5 WET Labs raw data can be converted in the user s software e g MATLAB or Excel using the calibration equation Relative Pressure dbar Output Slope Intercept Please note that strain gauge pressure sensors are susceptible to atmospheric pressure changes and should be zeroed on each deployment or profile The calibration equation for pressure above should be used first to get the relative pressure and the cast offset should then be subtracted to get the absolute pressure Absolute Pressure dbar Relative Pressure dbar Relative Pressure at Atmospheric Water interface dbar WARNING Do not exceed the pressure sensor s depth rating see calibration sheet Pressure Sensor Maintenance A plastic fi
29. tting filled with silicone oil provides a buffer between the pressure transducer and seawater The transducer is both sensitive and delicate Following the procedures below will ensure the best results and longest life from your pressure sensor Pressure 1s transmitted from the water to the stainless steel transducer diaphragm via a plastic fitting filled with silicone oil The inert silicone oil protects the pressure sensor from corrosion which would occur after long exposure to salt water The fitting will generally prevent the oil from escaping from the reservoir into the water However you may occasionally wish to ensure that oil remains in the reservoir on top of the transducer WARNING Never touch or push on the transducer l Thoroughly clean the top of the instrument 2 Completely remove the white nylon Swagelock fitting using a 9 16 in wrench 3 Check for obstructions in the tiny hole Blow clear with compressed air or use a small piece of wire 4 Wipe clean the o ring at the base of the Swagelock fitting 5 Screw the Swagelock fitting into the end flange until finger tight 6 Tighten it an additional 1 8 turn using a wrench only if necessary 7 Wipe up any excess oil ECO FLNTU User s Guide flntu Revision Z 13 May 2009 WET Labs 3 Theory of Operation The Environmental Characterization Optics ECO combination fluorometer and turbidity sensor allows the user to measure chlorophyll fluorescence at 470 nm and tu
30. y locate the instrument away from significant EMI sources ECO FLNTU User s Guide flntu Revision Z 13 May 2009 9 WET Labs Other than these basic considerations one only needs to make sure that the unit is securely mounted to whatever lowering frame is used and that the mounting brackets are not damaging the unit casing 4 5 Upkeep and Maintenance After each cast or exposure of the instrument to natural water flush with clean fresh water paying careful attention to the sensor face Use soapy water to cut any grease or oil accumulation Gently wipe clean with a soft cloth The sensor face is composed of ABS plastic and optical epoxy and can easily be damaged or scratched WARNING Do not use acetone or other solvents to clean the sensor 4 5 1 Bio wiper and Faceplate Cleaning and Maintenance The Bio wiper M and the copper faceplate need to be removed from the meter for thorough cleaning to maximize anti fouling capability l Be sure the meter is NOT powered or connected to a power source prior to uninstalling the Bio wiper M and faceplate WARNING Manually turning the motor shaft can damage the wiper motor and will void the warranty Make sure the Bio wiper is loosened from the shaft before attempting to rotate the Bio wiper 2 Remove bio wiper M Use the factory supplied 3 32 in hex key to loosen the screw that secures the wiper to the shaft on the instrument It may be necessary to remove the screw from the cla
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