NBB®-B in tubes user information sheet
Contents
1. User manual NBB B Tubes Product No 2 04723 646 page 1 3 1 Information Nutrient broth for the detection of Beer spoiling microorganisms esp Lactobacilli Pediococci Pectinatus and Megasphaera in yeast samples yeast turbid beer samples as well as for membrane and swab samples NBB Broth NBB B pH 5 75 0 15 is a ready to use broth for a fast and safe detection of beer spoiling microorganisms in the brewing industry Due to the selectivity of the NBB media the harmless accompanying flora as the contained yeast will be inhibited while beer spoilers are supported by a special growth component The yeast inhibitor was selected to operate after a short initial period time enough for the yeast to start fermentation This provides a favourable CO gt enviroment in the sample for an anaerobic incubation Therefore an anaerobic incubation while working with yeast samples is not necessary The NBB B is designed for the microbiological analysis of yeast samples but can also be used for sediments of unfiltered beer as a culture media for membrane filters as an enrichment media for trace contaminations for further analyses The tubes are filled with 10 mL NBB B and autoclaved to provide an immediate use Alternatively NBB B is available in 250 mL bottles under the Product No 2 04710 782 2 Handling Required Material Microbiological workbench Sterile swabs with wood sticks Incubator Application Handle the sam2. ethic abdina and sample 10 20 wii y Broth in ml of NBB B Yeast sediments tubes Yeast cloudy beers 95 of sample Green beer Concentrated P 2 04711 782 Unfiltrate broth 5 of NBB C Wheat beer Filtration of Clear beers 50 200 ml of Membrane filtered samples 2 04709 782 sample oe Filtration of Water rinsing water 50 200 ml of 2 04709 782 Membrane filtered samples sample Environmental air Sampling of airborne Direct sampling 2 04709 782 microorganism on agar plates Surfaces in filling plants Hygiene monitoring using iee 2 04706 782 swabs For the in house production of NBB A and NBB B using beer from own production NBB P Powder 2 04716 462 Laboratory accessories 9x 250ml 2 04710 782 glass bottle 28 C 20 x 10 ml 2 04723 646 ibe Smear swabs without tube 2 04725 444 ban page 3 3 Incubation Packaging Analysis Condition anaerobic qualitative 9x 250 ml PO glass bottle qualitative 9 x 250 ml l Sans glass bottle quantitative 9 x 250 ml gt i l B SECI t d 9 x 250 ml pans glass bottle quantitative 9 x 250 ml l o glass bottle qualitative 300g bag Version 4 0 19 June 2015 this version replaces all previous versions
3. may be sufficient in case of high contaminations to obtain a result Mostly several days are needed in case of trace contaminations or of very slow growing strains e g Lactobacillus lindner Observe the growth pattern during incubation time to obtain clear statements about the condition of your sample Evaluate your samples after 5 days of incubation for a final comparability of your results In case of trace contaminations or older autolyzing yeast cells from the tank bottom the indicator colour may not be evident enough to get a clear result In this case the samples have to be examined under the microscope 3 Storage and Packaging Information Packaging and Content unit 20 tubes each with 10 mL Storage Store at 4 8 C 40 46 F according to product specification Store under dry and dark conditions Do not freeze product Waste Disposal No dangerous good No hazardous material Please consider your local waste regulations Not inoculated broth can be disposed of with normal laboratory waste Inoculated and incubated samples are to be sterilized before disposal at a temperature of 121 C 250 F for 20 min Warnings Do not cook or freeze the product Version 4 0 19 June 2015 this version replaces all previous versions User manual NBB B Tubes Product No 2 04723 646 4 Related products Sample type Product Format Method Item no Broth in Yeast samples bottle 0 5 1 ml of
4. ples under sterile conditions to avoid secondary contamination Yeast samples can also be examined very handy with sterile swabs Collect your yeast sample approx 0 5 1 mL directly from several points of yeast container with a sterile swab place it into the tube with NBB B and break off the wood stick on the edge of the tube The screw Should not be tightened firmly during incubation to allow CO to escape from the tube Proceed in the same way with sediments of unfiltered beer Membrane filters after the filtration process can be rolled and transferred with sterile forceps into the tube and incubated under anaerobic conditions Incubation Place the test sample into an incubator between 26 28 C 79 82 F and incubate for 5 days Samples not containing yeast cells should be incubated under anaerobic conditions Incubate the enrichment probe for 2 days for further use with NBB A NBB B or molecular biological methods VIR PCR Version 4 0 19 June 2015 this version replaces all previous versions User manual NBB B Tubes Product No 2 04723 646 page 2 3 Evaluation If the contaminations are high enough and if the organisms are typical beer spoilers the colour of the indicator in the samples will turn from red to yellow Since the speed of the microbial growth depends on several factors initial cell count type physiological condition and origin of the germs degree of adaptation to beer an incubation time of one day
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