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Operating Manual 2010/2020

1. 66 7 2 2 Storing a Standard Curve AANEREN 68 7 2 3 Kinetic reading for extended range ELISA S 000 0 ceeeceeeeeeeeeeeeeeeeeeeeeeeseeeeeeeseeeaeereeneaeees 69 3 70 anthos 2010 2020 Operating Manual Safety Information All Warnings and Cautions in this document include an exclamation point a lightning bolt or a light burst symbol framed within a triangle Please pay special attention to the specific safety information associated with these symbols Warning and Caution Definitions The exclamation point symbol is an international symbol which serves as a reminder that all safety instructions should be read and understood before installation use maintenance and servicing is attempted When this symbol is displayed in this manual pay special attention to the specific safety information associated with the symbol WARNING A WARNING calls attention to a condition or possible situation that could cause injury to the operator CAUTION A CAUTION calls attention to a condition or possible situation that could damage or destroy the product or the operator s work Electrical Safety To prevent electrically related injuries and property damage properly inspect all electrical equipment priors to use and immediately report any electrical deficiencies Contact an Anthos service representative for any servicing of equipment requiring the removal of covers or panels High Voltage This symbol indicates the potential of an el
2. Del all Pit Del all Tst Del all Res Deletes all data in the selected highlighted list plates tests or results F3 Delete All Deletes all data all plates tests and results on the floppy disk F5 Refresh Re reads the data stored on the inserted floppy disk 5 7 5 3 Retrieve a Test from Disk Press lt menu gt and select the test lt menu gt Choose the retrieve option and press lt ENTER gt Choose single test or all tests and confirm with lt ENTER gt Now select the test to be retrieved and confirm with enter 53 70 anthos 2010 2020 Operating Manual 5 7 5 4 Export Plate Data as Text File Press lt menu gt and select the plate lt menu gt Choose the Export Text File option and press lt ENTER gt Choose single plate or all plates and confirm with lt ENTER gt Now select the plate to be exported and confirm with enter 54 70 Anthos 2010 2020 March 2010 Operating Manual anthos 2010 2020 6 Maintenance 6 1 Approved parts Except for the parts shown in the following list only parts supplied by Anthos or an authorized Anthos Distributor may be installed in or used with the anthos 2010 2020 e Fuses as specified in chapter 2 2 4 Rated operating conditions e Power cable CE marked power cable with connected protective earth and protective earth connector see also 2 6 e External Computer controlling the instrument see also 2 7 Connecting 2010 2020 with an e
3. Weight and Dimensions e Weight 2010 6 6 kg 2020 10kg e Dimensions width x height x length 2010 32 6 cm x 17 3 cm x 43 5 cm 2020 34 4 cm x 25 5 cm x 43 cm Environmental influences e Radio interference according to DIN VDE 0871 in progress threshold class B for interference voltage magnetic and electric interference field force e Acoustic power level according to DIN 45 635 part 19 in progress e Outer lighting influences Precaution avoid direct sunlight Electrical Rating e Consumption 2010 50VA fuse 1A T 2020 80VA fuse 1A T e Operating voltage 90 130V 180 250V e Frequency range 47 63 Hz auto sensing Interfacing e Parallel printer interface for IBM or EPSON compatible 9 and 24 pin printer and HP DeskJet 500 series 2020 only e Serial interface for remote control of basic measurement functions instrument status and setup by external PC Options e A wide range of filters e PC Software e Serial interface cable Anthos 2010 2020 March 2010 Operating Manual anthos 2010 2020 2 4 General features gt Absorbency Method Single channel well center triggered reading with post sample filtering eliminating the influence of ambient light Measurement Modes Single and dual wavelength reading Calibration prior to each measurement Plate centering system positions any 96 well microplate accurately under the optical path Quick Measurement Mode Mono bichromatic and kinetic m
4. Si anthos 2010 2020 Microplate Absorbance Reader 2020 sw version 2 0 up Operating Manual manual version 03 10 Biochrom Ltd 22 Cambridge Science Park Cambridge UK CB4 OFJ Tel 44 1223 423723 E Mail enquiries biochrom co uk anthos 2010 2020 Operating Manual Table of Contents Safety Jet FOE Ee EES 4 NR ET e E 7 1 1 une E NEE 7 2 Technical Information cciisoscsieescasceecseseseeedacerecsdesnaasecawedserdsasceaudascdawedersdeeviaassuendes 8 2 1 General Information regarding the Absorption Photometer 8 2 2 Metrological specification escc tices cxcesnocteeeived eaxateonexiesees aude SEN tees asin SEENEN 8 2 2 1 E tie Syster EE 8 2 2 2 Measuring PLOCESS so ececs EE 8 2 2 3 Measuring range specified according to DIN 58960 eeeceeeeeeeseeeeeeeeeeeeeeneeeeetneeeeees 8 2 2 4 Rated operating conditions oe eeeeeceeeenneeeeeeaeeeeeeaaeeeeeeaaeeeseeaaeeeeeeaaeeeseeaaeeeeeeaeeeeeeaas 8 2 2 5 Limits of error and tolerance me 9 2 3 General technical data seg sees ee EEN EEN ee SEN 10 2 3 1 Weight and DIMENSIONS keen 10 2 3 2 Environmental influences A 10 2 3 3 Ele CtriGals RAtinG R T Seu EETA TEE E ETE PLE E ENER EENEG 10 2 3 4 luc le GE 10 2 3 5 LEID ee 10 2 4 General EE 11 2 5 Scope of supply EE EE ea eh oes eee Oe 12 2 6 Installation requirements Environmental operating conditions ee 13 2 7 Connecting 2010 2020 with an external computer 14 2 8 Re packing to prevent damage during Irans
5. SXL may be replaced by fixed OD values or formulas This allows using stored standard curves with correction factors refer to 7 2 2 Storing a Standard Curve 67 70 anthos 2010 2020 Operating Manual 7 2 1 10 Use of Interpretation The Interpretation section provides not only cut off calculation based on optical density If the calculation base is set to the result name of the Transformation or to Concentrations also these results may be grouped by means of the cut off formulas Free names can be given to these groups up to five 7 2 1 11 Validation The validation formulas describe true conditions on basis of optical density values A bold warning will be stated on the printout if one of the validation criteria s is not fulfilled 7 2 2 Storing a Standard Curve Note The use of stored standard curves is not generally recommended or applicable for the quantitative evaluation of ELISA s It is explicitly the responsibility of the user to take advantage of the described software possibilities in the appropriate way Once a standard curve has been measured and OD results have been obtained by programming for standard S1 the response value as S1 for the standard S2 as S2 etc the curve can be copied to another test procedure the in the following way e Make aprintout of the measured plate with standards containing both concentration values and OD values e Copy the original test procedure to a new file by changing th
6. certificate Enclose the disinfection certificate in the reader package and attach the copy to the outside of the package so that it is clearly visible 6 3 Changing a Fuse 56 70 In case of malfunction the fuses in the mains filter next to the mains socket on the rear of the device can be checked and replaced e Disconnect the instrument from mains by unplugging the power cable Anthos 2010 2020 March 2010 Operating Manual anthos 2010 2020 Open fuse carrier next to the mains socket with a screwdriver Remove fuse Insert spare fuse included in supply and specified earlier in this manual Close fuse carrier Turn device on and check functioning In case of malfunction call a service technician 6 4 Filter Installation Note Before to starting the filter installation please read carefully the Safety Information at beginning of this manual Disconnect the instrument from the power source Move the instrument to the edge of a table so that it stands on three feet With a socket wrench unscrew the four hexagonal bolts on the bottom of the instrument holding the upper housing Do not turn the instrument upside down because the transport system could become misaligned Carefully lift the upper housing and disconnect the internal serial cable 2020 only Remove the upper housing Under the stepping motor you can see the filter wheel which is surrounded by a rubber band Choose the position where you want t
7. printout If the mean value of the replicates remaining after elimination constitutes the basis for the test evaluation the necessary conditions are to be defined in the chapter 5 3 12 14 45 19 Test Replicate Elimination Min Condition Tup Spl X lt 0 01 COON DU E GA Ma 10 ech wech _ N lt ce gt to activate formula input Screen 22 Replicate Elimination 42 70 Anthos 2010 2020 March 2010 Operating Manual anthos 2010 2020 gt Options Column Base Select the base values original measurement values or results of the relative calculation by selecting the Result Name or concentrations for the calculation for eliminating replicates Column Type Definition of the standard control blank sample Spl which is to be evaluated with the following condition Press lt ce gt select the corresponding position and confirm with lt enter gt Column Min Enter the minimum number of remaining replicates for samples only 0 is possible Column Condition Enter one ore more conditions which must be fulfilled by each replicate to avoid elimination Press lt ce gt to activate the input menu e All special positions defined in the plate layout standards controls blanks e Arithmetic functions 4 0 lt gt e Logical combinations NOT true if condition is not fulfilled AND true if all conditions are fulfilled OR true if one or more condit
8. quantitative evaluation is erroneous Cut Off Values state window is displayed if the qualitative evaluation is erroneous e g calculation of the cut off values cut off values are not increasing gt D Division is displayed if the argument left or right of the is missing Division by zero is displayed when a mathematical operation results in a division by zero gt F Formula Error state window is displayed if the evaluation formula is erroneous e g replicate elimination calculation of the test validation Function e is displayed if the argument of the Exp function is missing Function In is displayed if the argument of the Ln function is missing Function log base 10 is displayed if the argument of the Log function is missing Function power is displayed if one or both arguments of the Pow function are missing 58 70 Anthos 2010 2020 March 2010 Operating Manual anthos 2010 2020 Incorrect number format is displayed when a number with more than one comma is entered e g 12 34 5 Inversion is displayed when the argument after NOT is missing e g NOT L Left parenthesis expected is displayed when the left parenthesis of a formula is missing M Mathematical error state window is displayed if the quantitative evaluation is erroneous e g linear regression 4 paramter fit Multiplication is displayed if the argument left or right of the is missing N
9. 90 to 10 of transmission Measurements above 2 OD deal with less than 1 of the original light and will therefore have lower resolution and accuracy 7 1 2 Measurement at Specific Wavelengths Each substance to be measured has a specific transmission profile that indicates its concentration and composition A measurement with white light renders different sample concentrations in a certain spectral range only to a small amount and accordingly inaccurate A higher significance can be obtained by using only that part of the light spectrum which is relevant to prove the respective wavelength of the substance Due to this fact interference filters of a narrow banded wavelength spectrum are employed in this instrument Each substance has a certain absorption spectrum A measurement should be performed by selecting the correct filter for the maximum absorption of the sample because in this way the best differentiability of various sample concentrations can be reached We can also state that the differing absorption amplitude of two uniformly composed samples is a measure for the concentration For this reason measurements in the flank area of the spectrum are not very accurate and therefore to be avoided In this connection it has to be taken into consideration that when using interference filters wavelength tolerances of 2 nm from the nominal wavelength value may also result in inaccuracies Each sample has an absorption minimum not specific
10. Quick Plate Test Setup 14 17 25 Test Name EIJE Variables AL 2 568 Plate ID A2 1 500 Lotnumber ER Last Position 4 5 6 7 8 9 16 11 12 Scotch lt ce gt to activate text input Screen 26 Run Test gt Options e Test Name Press lt ce gt and choose the test to be started e Plate ID An automatic plate ID is available in default YYMMDDxx incremented from 1 to 99 To enter another plate ID press lt ce gt to delete the default 47 70 anthos 2010 2020 Operating Manual number and then scroll up down for text input Confirm with lt enter gt e Lot number Allows entering a lot number for this plate e Last Position Select the last position with the cursor keys e Variables In case those variables where defined during the test definition the values of these variables may be changed prior to run the test gt Function keys F5 Start Start the selected test with the above entered plate ID The progress of the test is displayed in the Test State window As soon as the test is measured and calculated confirm the Test State window with lt enter gt The measurement results are displayed 5 6 Measurement Results The measurement results are displayed immediately after the measurement was performed To re view the results of a measured plate select View in the plate menu Choose the plate to be re viewed and confirm with enter please refer also to chapter
11. an average or slope depending on the data reduction mode is calculated e g if 3 smoothing points are selected an average or slope for the values 1 2 and 3 is calculated then the average or slope for the values 2 3 and 4 is calculated etc gt Function keys F5 Start Meas By pressing this function key the measurement will be started with above defined parameters Data Reduction Methods Data Reduction is used to determine a single value per sample based on the results of a sequence of measurements over a period of time Delta O D Difference between two kinetic measurements in O D Average Slope Slope in O D min between the first and last kinetic cycle Only suited for linear reaction progressions with few cycles and measurement points Maximum Slope Maximum slope of the curve in O D min Maximum reaction speed The line with the highest slope is calculated The accuracy of this calculation depends on the number of measurement cycles selected Delta O D Maximum Slope Difference in O D between the first measurement and the center point of the maximum slope Calculation of the center point of the maximum slope Center point between the four measurement points of the regression line with the maximum slope Delta t Maximum Slope Time difference in sec between the first measurement and the occurrence of the center point of the maximum slope Anthos 2010 2020 March 2010 Operating Manual anthos 20
12. by a person other than anthos personnel or an authorized servicing agent e the warranty seals on the housing of the instrument are broken e parts and subassemblies are implemented which are not original from Anthos e anthos 2010 2020 serial number is no longer legible has been removed or altered e anthos 2010 2020 has not been installed in accordance with the instructions supplied e anthos 2010 2020 has been damaged during return transport due to wrong packing e g not in original packing material e anthos 2010 2020 was damaged due to wrong operation not according to the descriptions in the manual If a warranty is brought into operation Anthos shall repair or replace any defects which have resulted from faulty material or during production as it sees fit No costs shall arise for the client except cargo rates All components found in the original equipment or an adequate and full compatible alternative shall be available for a period of 5 five years after production This warranty refers to the obligations of Anthos and can only be amended upon the written consent of Anthos In its original condition the instrument meets all safety regulations for a risk less operation Anthos cannot be liable for damages or any resulting costs caused by unauthorized alterations repairs or modifications of the equipment 15 70 anthos 2010 2020 4 Installation Operating Manual 4 1 Un packing and System Set up
13. changes can be made so that the values are kept according to current kit lots 7 2 1 8 Quantitative Evaluation Concentration values are calculated by means of the calibration curve Linear regression is specifically applicable if the calibration curve is really expected to be straight on a linear or logarithmic concentration axis Point to Point and Cubic Spline curve fits are generally suited for non linear curves which are not completely straight but slightly bent However the Cubic Spline will not tolerate big jumps and turning points and will show a tendency to swing out at such occasions Point to Point may not be that accurate but is completely save and robust under all conditions For both the concentration axis has to be selected linear or logarithmic according to the sequence of standard points The 4 parameter Fit is a non linear regression especially suited for competitive assay formats Its theory assumes a central turning point and two asymptotes at the extreme ends The axis division is fixed to in log Zero concentration standards must not be included in the curve but in case of competitive assays they should be named as negative controls e g NC1 and may serve for the calculation of B By by means of the relational formula X NC1 100 The 4 parameter fit is also generally suited for non linear curves with sandwich assays If a turning point is not present it will adapt to the lower or upper branch of a sigmoid curve It
14. fit selectable scaling of X and Y axis lin log gt Combined evaluation All evaluation functions may be combined e g quantitative results can be classified using qualitative evaluation gt Validation and replicate elimination Conditions for automatic replicate elimination and control validation may be programmed within a test gt Functional Tests during Operation Light source detector and communication 2 5 Scope of supply e Measurement unit e Serial Cable and ADAP Software PC communication program e 4 filters 405 450 492 620 e Dust cover e User manual e Spare fuses e Power cord 12 70 Anthos 2010 2020 March 2010 Operating Manual anthos 2010 2020 2 6 Installation requirements Environmental operating conditions e The working area has to be flat dry clean and vibration proof and leave additional room for cables connections computer printer etc e The ambient air has to be clean and free of corrosive vapors smoke and dust The instrument is rated to Pollution Degree II and Installation Category Il e The ambient temperature has to range between 15 C and 40 C for operation and between 25 C and 50 C for storage e Indoor use only Humidity has to range between 15 and 85 e Height over sea level during operation up to 2000 m Please Note Only use the power cable supplied with the instrument or a power cable with protective earth connection carrying the CE mark The p
15. is more stable than the Cubic Spline and will never overshoot but may need slightly longer calculation times Extrapolation set in allows calculating sample concentration also in a small area below and above the extreme standards Extrapolated results are marked on the printout with an X Results out of range are shown with the prefix gt or lt to the highest or lowest valid concentration border of extrapolated area A few percent extrapolations are recommended in order to get both replicates of extreme standards into the valid area for results otherwise the mean of replicates is the borderline 7 2 1 9 Set up of Standards and Axis Division Standards have to be entered in increasing order of concentrations always lowest concentration S1 In case of competitive assays this will produce a falling curve If the standard series is produced by a more or less constant adding e g 10 20 30 or even 10 20 40 70 the axis division is linear If there is a multiplication dilution factor e g 2 4 8 16 or 10 33 100 330 the axis division is logarithmic Zero concentration standards must not be used on a logarithmic scale for mathematical reasons If such a standard sequence is suggested in the kit instructions do not use the zero standard for the curve recommended or set it to a small positive value two logs below the second standard e g 0 02 2 4 85 The identifiers for standards on the plate S1
16. this screen the measured values for every well are displayed gt Function keys F1 Well Graph Displays the reaction curve of the selected well F2 Plate Graph Displays the reaction curves of all well of the plate F3 Prev RawDat Displays the raw data of the previous measurement F4 Next RawDat Displays the raw data of the next measurement F5 Select Screen 5 6 3 Measurement Result Screen Displays the measured values in the plate layout gt Function keys F5 Select Screen 5 6 4 Plate Layout Screen In this screen the plate layout is displayed Additionally certain wells can be rejected R to be set unset with the lt ce gt key This option is meant to be able to reject so called outliers and to be able to re calculate the plate without these wells If all replicates of one standard are deleted the plate can be re calculated without this standard 49 70 anthos 2010 2020 Operating Manual gt Function keys F1 Re Calc To re calculate the plate in case that the rejection state of one ore more wells had been changed F5 Select Screen 5 6 5 Relational Result Screen Is referred to under the name given in the test definition for the relational results The recalculated with the formula defined in the test parameters for relative calculation results are displayed on the screen gt Function keys F5 Select Screen 5 6 6 Quantitative Results Screen This scr
17. to the measurement value which can be deducted from the measurement value automatically by choosing a suitable reference wavelength This kind of measurement is referred to as bichromatic 65 70 anthos 2010 2020 Operating Manual 7 2 Evaluation applications 7 2 1 Quick reference for test programming 7 2 1 1 Bichromatic reading It is highly recommended to use a reference reading for subtracting unspecific signals Suited measurement and reference filters are Substrate Measurement Filter Reference Filter PNPP 405 nm 620 nm TMB 450 nm 620 nm OPD 492 nm 620 nm ABTS 405 414 nm 492 nm 7 2 1 2 Blank against Air An automatic self calibration determination of 100 and 0 transmission signals is performed by the reader for each measurement channel and each particular filter previous to readings This is equivalent to the blanking against air which is often mentioned in kit instructions 7 2 1 3 Shaking Function If available it is highly recommended to use a short shaking cycle e g 5 seconds at medium speed previous to measurements This will homogenize the color solution color develops at the edges of the well and will produce an equal and stable liquid meniscus in all wells As a result accuracy and reproducibility are significantly improved 7 2 1 4 Plate Layout Singles or replicates of blanks standards controls and samples may be distributed freely across the plate There a
18. 10 2020 e Delta t Relative Time in sec from the first measurement to reaching a set increase decrease in O D e Delta t Absolute Elapsed time from one pre selected threshold value to another gt Function keys F5 Start Meas Start the measurement with the selected filter s 5 3 Test Definition Programming of tests evaluation modes Press lt menu gt and select Test and then New to define a new test 13 58 44 Define Test Test Name Measurement Mode Pre Waitin lt ce gt or lt 4 gt lt h gt to change Screen 7 Define Test Since all programming options together are seldom used during one test optional settings may be skipped Ha special point e g quantitative evaluation is required the question regarding the use of such function must first be answered checked X press CE before the corresponding menu e g Standard points and quantitative evaluation is displayed 5 3 1 Test Identification and Pre Measurement Functions e Test Name Press lt ce gt to activate the text input mode Now the text position left right and the required letters up down can be selected with the cursor keys e Measurement Mode 25 70 anthos 2010 2020 Operating Manual Select the measurement mode e Single Point Photo e Kinetic Photo e Transformation Mark X this option if relational calculation is required all measurement values are re calculated wit
19. 4 2 Power On 16 70 The original packing has been especially designed to protect the instrument during transportation It is therefore recommended to keep the original carton with its foam parts and the accessories box for re use in case of future shipments Warranty claims are void if improper packing causes transport damages e Check the box for any visible damage during transportation In case of damage inform your supplier immediately and keep the damaged packing e Place the device on a suitable working surface e Remove the transportation lock foam part from the plate holder e Connect the serial cable to PC e Connect power cable to standard mains plug e Switch on main switch rear left side The anthos 2010 is immediately ready to stand by for remote control The anthos 2020 performs the complete initialization and shows the main menu after approx 30 seconds The power indicator is located on the front left side of the unit and is marked with the following symbol Q The power indicator may show 2 different states Green light Instrument is switched on ready for operation Green flashing A problem during initialization was encountered The instrument is not ready for use Not illuminated Instrument is not ready for operation Anthos 2010 2020 March 2010 Operating Manual anthos 2010 2020 4 3 Software Installation 4 3 1 ADI and ADAP ADAP Basic is remote control software for Anthos instruments
20. 5 7 1 The first screen which is displayed is the Test State screen This screen shows the information concerning the evaluation of the plate To return to the main menu press lt stop gt twice gt Function keys The function keys lt F1 gt to lt F4 gt have different functions depending on the data displayed on the screen F5 Sel Screen Depending on the performed measurement different screens are available 5 6 1 Info Screen The screen appearing first after each measurement or re evaluation of data is the info screen showing the plate name test name lot number measurement date and time and the variables only if variables were defined for this test 48 70 Anthos 2010 2020 March 2010 Operating Manual anthos 2010 2020 If the evaluation of the test run was invalid a list of error messages is displayed gt Function keys F1 Print Prints information concerning the test and measurement time Also the results are printed according to the settings in the test definition please refer to chapter 5 3 13 1 Make sure a printer is connected and on line and that the correct printer is selected in the system setup F2 Save As Appears only if a plate had been re evaluated This function enables to store the plate with a new name F5 Select Screen 5 6 2 Raw Data Screen This screen is only displayed for measurements where raw data are available e g kinetic measurement results In
21. Date format Select between 24 h and 12 h am pm e Shutdown Press this button for shutdown Quick Plate Test 09 19 27 AM System Floppydisk Maintenance Remote control Data Transfer Update Language Date Format Shutdown Screen 4 Further option of the setup menu 21 70 anthos 2010 2020 Operating Manual 5 2 QUICK measurements without test programming and storage Quick Plate Test Setup 12 43 56 Quick Select one of the following measurement modes Single Pt single point photometric measurement Kinetic kinetic photometric measurement Screen 4 Quick Menu gt Function keys F1 Single Pt Perform a single point mono or bichromatic absorbency reading F3 Kinetic Perform a kinetic absorbency reading 5 2 1 F1 Single Point Quick Plate Test Setup 12 48 00 Quick Single Point Photo Meas Filter nm KI Ref Filter nm BSE lt 4 gt lt h gt to select Screen 5 Single Point Measurement 22 70 Anthos 2010 2020 March 2010 Operating Manual anthos 2010 2020 gt Options e Measurement filter highlight the desired measurement filter and confirm with lt enter gt e Reference filter highlight the desired reference filter as above if no reference filter is required select gt Function keys F5 Start Meas Start the measurement with the selected filter s 5 2 2 F3 Kinetic T Quick Plate Test Setup 13 18 52 Qu
22. Free configuration of the blanks on the entire plate The mean value of all blanks in each row is deducted from each sample of this row Example Setting blanks on plate position AO1 and A08 Mean value of A01 and A08 A01 A08 2 this mean value is deducted from each sample of this row A02 A03 A04 A05 A06 A07 A09 A10 A1 1 A12 e Column Free configuration of the blanks on the entire plate The mean value of all blanks in each column is deducted from each sample of this column Example Setting blanks on A01 BO1 and DOT Mean value of A01 B01 and D01 A01 B01 D01 3 this mean value is deducted from each sample of this column C01 E01 F01 G01 H01 e Fill Direction Filling pipetting direction horizontal vertical Note Groups of replicates are sorted according to their replicated direction AND the filling direction e AG control strips Antigen control strip setting A control sample which is automatically deducted is assigned to each sample The AG control strip is always labeled AGC on the printout The control well sample positioning depends on the filling direction and the sample control setting combination of the reader e Control Well Select whether the control well is located before or after the sample 27 70 anthos 2010 2020 Operating Manual e Direction Direction horizontal vertical of the AG control strips relative to the sample if AG control strips and replicates are used together the filling d
23. Name defined in chapter 5 3 8 e Concentration x Enter the concentration value to be plotted on the x axis of the curve graphic Constants of up to 12 digits may be entered on the numerical keyboard It is also possible to define formulas containing all the set controls 36 70 Anthos 2010 2020 March 2010 Operating Manual anthos 2010 2020 standards numerical constants and variables for the concentration value To enter a new formula press lt ce gt scroll with the cursor keys up and down and select the required operator variable with the corresponding function key The concentrations of the standards have to be entered in increasing order Response value y Refers to the absorption value to be plotted on the y axis of the curve graphic Its default formula is the identifier of the standard points S1 S2 and corresponds with the OD values of the standards on the plate However it can also be defined by an algebraic formula containing all the set controls standards numerical constants and variables To enter a formula press lt ce gt scroll with the cursor keys up and down and select the required operator variable with the corresponding function key Units of Concentration Press lt CE gt and enter a name by scrolling through the alphabet Curve Fit Select a suited curve fitting formula Depending on the type of curve calculation a minimum number of standards must be set Linear regression 2 sta
24. Number length exceeded is displayed when number is longer the 14 characters O Overflow is displayed when the result of a mathematical operation exceeds the maximum of the positive range e g Exp 1000 P Parameter expected XXX is displayed if the argument left or right of an operator is missing eg 1 gt Precondition is displayed when the result of a mathematical operation is out of the specified range e g the response value for a 4 parameter fit is less or equal the lower asymptote R Result is displayed if the entered formula gives more or less the one result Result is a singularity is displayed when the result of a mathematical operation would be a singularity e g Pow 0 2 59 70 anthos 2010 2020 Operating Manual Right parenthesis expected is displayed when the right parenthesis of a formula is missing S Spline state window is displayed if the quantitative evaluation is erroneous e g curve not definite Standard Point Calculation state window is displayed if the quantitative evaluation is erroneous e g calculation monotony Subtraction is displayed if the argument left or right of the is missing T Total loss of significance is displayed when the digits behind the comma are not correct Type mismatch is displayed when the entered formula is not correct e g X gt 0 5 AND 1 U Underflow is displayed when the result of a mathematical oper
25. and maybe supplied with 2010 and 2020 ADAP Plus and ADAP Expert are evaluation software packages offered by ASYS Hitech e Insert the CD ROM and open Start Here html on the CD e Install ADI and ADAP software as described in this file e Connect instrument and PC with the serial cable provided with the instrument e Switch on the instrument 2010 is ready for remote control if the green LED is on 2020 has to be switched to Remote Control in the Setup Menu Refer to 5 1 4 Setup Menu Options e Start ADAP and set up the instrument in ADAP Software e All further steps are described in the ADAP User Manual 4 4 Switching off When switching off the anthos 2020 make sure that the command F5 Shutdown or Shutdown in the Setup menu is always used If this is not used specific settings will NOT be stored Wait for the message Switch the instrument off and then turn off the unit with the mains switch 17 70 anthos 2010 2020 Operating Manual 5 2020 On Board Software 5 1 Controls and System Setup 5 1 1 Description of the key functions Quick Plate Test Setup Main Anthos 2020 Version 1 8 Filters 405nm 450nm 492nm 550nm 620nm lt stop gt during a measurement or in the test definition this key aborts the current task It can also be used to cancel dialogues lt prev gt back to the previous dialogue element lt enter gt confirms the text numerical entry and
26. anufacturer otherwise the protection provided by the equipment may be impaired Chemical and Biological Safety Normal operation of the 2010 and 2020 Absorbance Detectors may involve the use of materials that are toxic inflammable infectious or otherwise biologically harmful When using such materials observe the following precautions e Handle infectious samples according to good laboratory procedures and methods to prevent the spread of disease Wear protective gloves e Observe all cautionary information printed on the original solutions containers prior to their use e Dispose of all waste solutions according to your facility s waste disposal procedures e Operate the 2010 and 2020 Absorbance Detectors in accordance with the instructions outlined in this manual and take all the necessary precautions when using pathological toxic or radioactive materials e Splashing of liquids may occur therefore take appropriate safety precautions such as using safety glasses and wearing protective clothing when working with potentially hazardous liquids e Use an appropriately contained environment when using hazardous materials e Observe the appropriate cautionary procedures as defined by your safety officer when using flammable solvents in or near a powered up instrument e Observe the appropriate cautionary procedures as defined by your safety officer when using toxic pathological or radioactive materials e Wash your hands thor
27. arameter on screen and printout Default plate ID Number of variables increased to 24 Version 1 8 Addition of time format settings for 12hours AM PM or 24 hours There is no released version 1 6 and 1 7 the numbering was adjusted for running parallel with Lucy 3 Version 2 0 Remain in status remote when shut down and interruption of remote control only by STOP key Save button in System Setup 63 70 anthos 2010 2020 Operating Manual 7 Appendix 7 1 7 1 1 64 70 Principles of Photometry Measurement Light is electromagnetic wave radiation Rays from 100nm to 400nm are defined as the ultraviolet spectrum of light Only rays in the range from 400nm to 780nm are visible for the human s eye rays with longer wavelengths are called infrared Color impressions are caused by reflection of electromagnetic waves striking the surface of material substances Substances absorb the complementary spectrum of their visual perceptible color Hence green plants look green since they absorb red light light of a wavelength perceived as red e g round about 750nm A photometer is an optoelectronic measuring device to determine the amount of light absorbed at a specific wavelength Therefore a photometer can be used to determine the concentration of a certain light absorbing substance in a colored solution Absorbency reading Experimental measurements return the value of transmission T Transmission is defined as the percentag
28. ation exceeds the maximum of the negative range e g Log 1 Unknown character X is displayed when the formula contains an unknown character e g X this is only possible when the instrument is controlled with an external PC V Variable not found is displayed when formula contains a variable which is not defined 6 5 2 Device and Communication Error messages 60 70 gt sb1 1 FILTER ERROR If the fw value in NORMAL MEASUREMENT command reefer chapter 10 7 does not match the fw from the anthos 2010 2020 lists the FILTER ERROR flag and ALL ERROR flag are set This is the information that the requested filter is not installed in the anthos 2010 2020 Also a movement of the filter wheel during a NORMAL MEASUREMENT Anthos 2010 2020 March 2010 Operating Manual anthos 2010 2020 command or an error in the SET FILTER command can cause this error sb1 2 TRANSPORT INIT ERROR The error may occur during the transport initialization and is reported through this status flag It indicates that the transport system is not in the load position A defective motor transport mechanism or sensor could be possible reasons for this error sb1 3 PRINTER BUSY ERROR Before a byte is transmitted to the printer the BUSY line is checked A 0 5 seconds timeout is set for waiting for the printer Possible reasons printer is OFF LINE no paper sb1 4 PRINTER ACKNOWLEDGE ERROR Indicates no ACK response from printer for trans
29. be repeated gt sb2 7 ALL ERROR This is a common error status bit which is logical OR of all the individual error status flags This is a read only status bit useful for a quick check if any error status flag is set The GET STATUS command clears this and other error status bits 62 70 Anthos 2010 2020 March 2010 Operating Manual anthos 2010 2020 6 6 Software History 2020 Firmware 6 6 1 6 6 2 6 6 3 6 6 4 6 6 5 6 6 6 This chapter lists changes in new software versions compared to the previous versions The first released version was 1 1 Version 1 2 Implementation of the floppy disk functions data storage on the internal disk drive of the anthos 2020 Version 1 3 Improved printing speed Version 1 4 Introduction of editable screen text and language versions All text appearing on screens and printouts is listed in a text file and can be translated to different languages Currently implemented are English and German Version 1 5 Additional screen showing CV Display of CV in standards table Display of percentage difference between label and back fit concentration on the printouts Display of units of concentration on screens and printouts Names of standard points SO is possible Calculation and display of data in case of invalid status Information about errors on the printout Optional printout of validation formulas Decimal digit for Min Correlation Display of the extrapolation p
30. cally controlled Tungsten halogen lamp e spectral apparatus Di electrical interference filters Filter wheel can hold up to 8 filters four filters supplied as standard 405 450 492 620 nm e Radiation receptor single silicon photodiode 2 2 2 Measuring process e Measuring class index absorption corresponds to O D optical density measuring geometry Zero point setting self calibrating Concentration test comparison value process Measured value test reference value process Control measurements automatic during each measuring operation 2 2 3 Measuring range specified according to DIN 58960 e Spectral absorption factor A A 0 2 5A The limits of error and tolerance limits listed in Section 2 2 5 may be exceeded beyond this effective evaluation measuring range and for rated operating conditions other than the following section 2 2 4 Rated operating conditions e Measured wavelength 400 lt A lt 750 e Blank value is deducted depending on the application under the testing regulation to be defined by the user from the measured value of the samples available on the microplate Since this value highly depends on the type of plate used a blank cell is placed at a position on the 8 70 Anthos 2010 2020 March 2010 Operating Manual anthos 2010 2020 microplate to be specified by the user and measured together with all other samples in one operation Warming up time Ready for operation immediately after swi
31. ctor to the plug using the corresponding screws on the connector Connect the other end of the serial cable with the plug marked RS232 on the rear side of the instrument Lock the connector to the plug using the corresponding screws on the connector Please Note Models 2010 and 2020 use different cables Only use the original cables supplied by anthos for 2010 order number S VZ 000 001 or 2020 order number S VZ 000 002 In emergency a standard 0 modem cable for PC PC connection may work with Model 2020 only but not with Model 2010 All other cables with connectors looking identical must not be used to try to establish communication 2 8 Re packing to prevent damage during transport 14 70 The original packing has been specially designed for this equipment It is therefore recommended to save the original carton with its foam parts and accessories box Warranty claims are void if improper packing causes transport damages Anthos 2010 2020 March 2010 Operating Manual 3 Warranty 3 1 Liability anthos 2010 2020 A warranty period of 12 months shall be granted to the original buyer of the anthos 2010 2020 This warranty shall lose effect if e anthos 2010 2020 is not used in the defined scope of application e anthos 2010 2020 has obviously been damaged by external influences which are not in accordance with the provisions for the nominal range of use e anthos 2010 2020 has been modified or parts exchanged
32. e name and editing the test a copy will be stored under the new name e Putin the OD values of the measured standards S1 Sx as default values for Variables V1 Vx e The layout does not need to contain a real standard anymore Fill the plate just with samples e As response values enter the variables V1 Vx instead of the standards S1 Sx When starting a reading with the new test the variables are displayed and may be updated to the last reading of a standard series In order to correct plate to plate variations of the assay it is highly recommended to have at least one real standard on the plate by which a correction factor is calculated The correction factor is the actual measurement value divided by the initial measurement value of the repeated standard Example A plate with 5 standards is programmed Standard Points S1 S2 S3 S4 S5 Concentration 0 1 1 10 100 1000 Values Response Formulas S1 S2 S3 S4 S5 68 70 Anthos 2010 2020 March 2010 Operating Manual anthos 2010 2020 A new test is programmed The measured OD s of the standards are entered to the variables Standard Points S1 S2 S3 S4 S5 Response Values 0 3 0 6 0 8 0 9 1 0 OD Put to Variables V1 V2 V3 V4 V5 Only standard S3 is used on the plate layout of the new test It is named C1 control and its measured value is used to calculate the correction factor for all other standa
33. e ratio between the total available light energy at the detector measured through air and the residual luminous energy after sample transmission at a specific wavelength T Mlo I Light intensity after passing through the sample In Initial light intensity Transmission has no linear relation to concentration therefore Absorbency also called Optical Density is calculated from Transmission by the formula A OD log T or A OD log 1 T Transmission is not related to layer thickness path length of the light and sample concentration in a linear way If one layer of a certain material allows 10 transmission doubling either path length or concentration would bring down the transmission to 1 and doubling both would bring it down to only 0 01 logarithmic relation Absorbency is related in a linear way to layer thickness and sample concentration according to the Bouger Lambert Beer law E OD c d e c the concentration of the sample to be measured d the layer thickness of the sample to be measured e the molar extinction coefficient The following graph illustrates the relation between Absorbency sample concentration and the light transmitted and measured at the detector Anthos 2010 2020 March 2010 Operating Manual anthos 2010 2020 Relation OD Transmission ZS S S 15 Optical Den sity It is important to keep in mind that the best measurement range of Absorbency is from 0 1 to 1 OD or
34. easurement with data output in OD values on display and printer Programming and Evaluation When programming a test the software leads you through a logical sequence of screens covering all necessary steps Flexible plate layout functions Selectable blank validity auto replicate mode and filling direction All types of controls and standards can be positioned freely in the plate template Evaluation possibilities All evaluation methods can be applied to measurement values OD or relational values Results can be viewed on the screen and outliers rejected prior to printout Controls and standards Can either be related to the measured absorbency the result of a relational calculation defined by a formula or fixed value Therefore fixed or floating cutoffs and standard curves e g single point calibration can easily be programmed Powerful formula parser Measurement values of all controls and standards set in the plate layout as well as constants variables and mathematical operators can be used to create a formula for calculation of cutoffs standards e g preset standard 11 70 anthos 2010 2020 Operating Manual curves single point calibration and relational values e g B BO gt Qualitative evaluation Up to 5 ranges 4 cutoffs individually named up to 10 equal sized ranges or ranging between 2 cut offs gt Quantitative evaluation Point to point linear regression cubic spline 4 parameter
35. ectrical shock hazard existing from a high voltage source and that all safety instructions should be read and understood before proceeding with the installation maintenance and servicing of all modules e Voltages dangerous to human life are present in this device Before removing any covers disconnect the device from the power source e Ensure that the power cord supplied with the unit is used e The power cord may only be inserted in a socket outlet provided with a protective ground earth contact The protective action must not be negated by use of an extension cord without a protective grounding contact e Do not replace fuses without first removing the main power cord Ensure that only fuses with the required rated current and of the specified type are used for replacement The use of makeshift fuses and the short circuiting of fuse holders is prohibited e When the apparatus is connected to the main power source the opening of the covers or removal of components is likely to expose live parts The device shall be disconnected from all voltage sources before it is opened for adjustment or repair e Any adjustment or repair of the opened apparatus under voltage should be avoided but if necessary it must be carried out by qualified service personnel who are aware of the hazards involved 4 70 Anthos 2010 2020 March 2010 Operating Manual anthos 2010 2020 e Use the equipment only in the intended manner and as specified by the m
36. ed Press lt ce gt All standards controls arithmetic functions and variables which can be used in the cutoff formula appear in the display menu Enter numbers with the keyboard or select the desired characters with the cursor and the function keys Confirm complete formula by pressing lt enter gt Group Name x All values falling in this group must be within the range defined by the previous and the next cutoff CV Press lt ce gt to activate the text input and select the desired characters with the up down cursor keys 41 70 anthos 2010 2020 Operating Manual Confirm with lt enter gt If Interpretation Mode Auto ranging or Interpretation Mode ranging the intermediate range between two cutoff values cannot be individually named since either the group or a percentage is automatically printed as the name This range is referred to as Special gt Function keys F4 Back Return to the previous screen F5 Next Proceed to the next screen 5 3 11 Replicate Elimination This function serves to eliminate individual replicates which do not fulfill a certain user defined condition After the first elimination the mean value of the remaining replicates is re calculated and the condition is re examined If necessary the elimination cycle is repeated If a minimum number of replicates to be defined are still available in the end the test is valid if not it is marked invalid on the
37. eeeeteaeeeeeseeeeeeeseeeeeeeeneaeeeeeeeaees 33 5 3 8 Relative G aleulationiccass cagtec cciecessst Sege eege Seege deeg 34 5 3 9 Quantitative Evaluation cccccccecceeceeececeeeeeeaeeeeaaececeeeceaeeeceaeeseaaeseneeeseaeeesaeeeeeeseeeeesaas 36 5 3 10 Qualitative Evaluation eeccececceceeeeeeeeeeeeeee cee eeeeaeeeeeeeseeeecaaeeeeaaeseeeeeseeeesaeeseaeeseeeeess 40 5 3 11 Replicate Elminaton cee eccceeecceeeeenneeeeeenneeeeeeaeeeeeeaaeeeeeeaaeeeeeeaaeeeeeeaeeeeeeaeeeenenaeeeeee 42 GECKEN e Er Le EEN 43 5 3 13 Printing amp Safeguarding the Test in the Test Memory c ccccssececeeseeeeeessteeeeesseeeeees 45 2 70 Anthos 2010 2020 March 2010 Operating Manual anthos 2010 2020 5 4 Editing and Clearing of Defined Tests ccccceeeieeeeeneeeeeeeeeeeeeeenneeeeeeeeeeneee 46 5 4 1 Edit Test Changing a Test Already Programme 46 55 PRU ebe ieee cane E alee A dae OE Ae Nh aarti EEN 47 5 6 Me surement keller 48 5 6 1 NIO CT TT 48 5 6 2 Raw Data ereen eer REN aa iia a a RUE 49 5 6 3 Measurement Result Green 49 5 6 4 Plate Layout reene caida iam Aa i ah A hates 49 5 6 5 Relational Result Green 50 5 6 6 Quantitative Results Screen ccceeeeeeeeceeceneeeeaeeeeneeseeeeeceaeeeeaaeseeaeeseeeeseaeeeeaaeseeneeenaes 50 5 6 7 Standard Data Screens tacit ancient ata Agi Age tile damit 50 5 6 8 Qualitative Results Screen cccceeeeseeeececeeeeeeeaeeeenaeeeeeeceaeeesaaeseeaeeeeeeeesaeeeeaesseneeesaes 50 5 6 9 GV Y
38. een is only available if quantitative calculation was defined in the test parameters It shows the results of the quantitative calculation The name of this screen will change to the concentration unit name e g MG ML which was Set up in the test definition gt Function keys F5 Select Screen 5 6 7 Standard Data Screen This screen is only available if quantitative calculation was defined in the test parameters It shows the data of the standard curve gt Function keys F1 Re Calc To re calculate the plate in case that curve parameters had been changed F3 Graphics Displays the standard curve according to the defined parameters F5 Select Screen 5 6 8 Qualitative Results Screen This screen is only available if qualitative calculation was defined in the test parameters It shows the results of the qualitative calculation 50 70 Anthos 2010 2020 March 2010 Operating Manual anthos 2010 2020 gt Function keys F5 Select Screen 5 6 9 CV Screen This screen shows the percentage correlation of variation of replicate standards controls and samples The value is shown on the upper left plate position of replicates gt Function keys F5 Select Screen 5 7 Plate Data Storage After each measurement with a pre programmed test all measurement and evaluation data all test parameters and the plate identification number are stored in the plate memory H the memory is full the res
39. ened in ethanol or a chloric solution Put non lint tissues that have been moistened in ethanol or a chloric solution onto the plate transport mechanism and let it soak for 30 minutes When a chloric solution has been used carefully wipe off the entire reader with non lint tissues that have been moistened in water Dry the reader by wiping it off with non lint tissues Disinfection Before the reader is returned to the distributor it must be disinfected and a disinfection certificate must be completed The following procedure must be used for disinfecting the reader 1 2 3 7 8 9 Switch off the reader and disconnect it from the mains power supply When used disconnect the reader from any accessories printer and PC Carefully wipe off the entire reader with non linting tissues that have been moistened in a protein degrading mild detergent or a saline solution Carefully wipe off the entire reader with non lint tissues that have been moistened in ethanol or a chloric solution Put non lint tissues that have been moistened in ethanol or a chloric solution onto the plate transport mechanism and let it soak for 30 minutes When a chloric solution has been used carefully wipe off the entire reader with non lint tissues that have been moistened in water Dry the reader by wiping it off with non lint tissues Pack the reader in its original packaging Complete a disinfection certificate and make a copy of the
40. f the plate transport risk of injury e Keep the 2010 and 2020 Absorbance Detectors work area clear to prevent obstruction of the movement 6 70 Anthos 2010 2020 March 2010 Operating Manual anthos 2010 2020 1 Introduction Picture 1 anthos 2020 and anthos 2010 The Readers anthos 2010 and anthos 2020 are designed for all applications of endpoint and kinetic absorbency reading in 96 well microplates anthos 2010 is controlled by a PC and ADAP Software anthos 2020 is equipped with a built in PC display and keyboard and with comprehensive software functions on board for performing measurements and data evaluation When operated in remote control it works identical to anthos 2010 but in addition files stored on board measurement data test definition and evaluated result files in readable ASCII text format can be exchanged with a desktop PC by means of ADAP Software functions This manual covers both model version of the instrument Chapter 5 2020 On Board Software applies only for anthos 2020 1 1 Intended use The Readers 2010 and 2020 are intended for general laboratory and research use only 7 70 anthos 2010 2020 Operating Manual 2 Technical Information 2 1 General Information regarding the Absorption Photometer Type 1 channel single beam absorption photometer and discontinuous wavelength feed filter 2 2 Metrological specification 2 2 1 Measuring system e Radiator pre adjusted automati
41. h Yes and lt enter gt or discard it by selecting No and lt enter gt Note Please make sure to have printouts of the result available prior to deleting it 5 7 5 Floppy Disk Data Management 5 7 5 1 Save a Test to Disk 52 70 Press lt menu gt and select the test menu Choose the store option Now copy test or copy all tests or shift test or shift all tests can be selected Copy The copy option copies the data from the anthos 2020 to the disk the data is now available on both the instrument and the disk Shift The shift option moves the data from the storage of the anthos 2020 to the disk the data is now available only on the disk Select copy test and confirm with lt ENTER gt to copy a single test to disk select copy all tests to store all tests to disk Now select the test to be stored to disk and press lt ENTER gt Anthos 2010 2020 March 2010 Operating Manual anthos 2010 2020 5 7 5 2 Delete a Test from Disk Quick Plate Test Setup Floppudisk Maintenance Plates Tests Results i lt a gt lt gt to select Screen 27 Floppy disk Maintenance Press lt menu gt and select Floppy Disk Maintenance in the setup menu Select highlight the data you like to delete and press one of the following gt Function keys F1 Del Plate Del Test Del Result Deletes the highlighted data plate test or result F2
42. h a defined formula please refer to chapter 5 3 8 Relative Calculation e Quantitative Evaluation Mark X this option for quantitative evaluation e Qualitative Evaluation Mark X this option for qualitative evaluation e Replicate Elimination Mark X this option if automatic replicate elimination is required e Pre Waiting Mark X this option to activate a waiting time before the measurement starts e g for kinetic measurements where the reaction starts only after a certain time e Variables Mark X this option if special variables for calculations are to be defined e g for the definition of lot specific factors or concentrations e Test Validation Mark X this option if an automatic test validation is wanted The criteria for the validation can be defined later on gt Function keys F5 Next Proceed to the next screen 5 3 2 Plate Layout Pattern 14 67 19 Test Plate Layout Pattern Blank Validity Control Well Direction DI AG Control Strips Fill Direction Number Direction IX Auto Replicates wells Pattern 2 3 4 5 6 7 romm NnwWD lt a gt lt gt to select Screen 8 Plate Layout Pattern 26 70 Anthos 2010 2020 March 2010 Operating Manual anthos 2010 2020 gt Options e Blank Validity Validity of the blank value positions on the plate e Plate Mean value of all individual blanks is automatically deducted from all other measurement results e Row
43. ick Kinetic Photo Meas Filter nm EGE Ref Filter nm BISON Interval sec Number Of Cycles Data Reduction lt 4 gt lt h gt to select Screen 6 Kinetic Measurement gt Options e Measurement filter highlight the desired measurement filter and confirm with lt enter gt e Reference filter highlight the desired reference filter as above if no reference filter is required select Usually no reference filter is used for kinetic data since a reference reduction only shifts the relevant reaction process up or down which has no effect on the results of the data reduction e Interval interval setting in seconds from the beginning of one measurement cycle to the beginning of the next cycle minimum time 45 seconds for single wavelength 90 seconds for dual wavelength e Number of cycles number of measurement cycles to be performed press lt ce gt and enter number using keyboard 23 70 anthos 2010 2020 5 2 2 1 24 70 Operating Manual Data Reduction Possible choices are e Delta OD e Average Slope e Maximum Slope e dOD Max Slope e dt Max Slope e dt Relative e dt Absolute Please refer to chapter 5 2 2 1 for detailed information on the different data reduction modes Smoothing Points Depending on the selected data reduction mode smoothing points can be defined To reduce peaks in the result a number of values can be defined of which
44. ions are fulfilled XOR true if only one of the conditions is fulfilled e Variables please refer to chapter 5 3 4 e X represents each individual replicate of the type selected Move cursor to select a row and confirm with the corresponding function key Delete the last entry with lt ce gt Finally re confirm the formula with lt enter gt gt Function keys F4 Back Return to the previous screen F5 Next 5 3 12 Validation Proceed to the next screen If a programmed condition is not fulfilled the entire test is marked invalid 43 70 anthos 2010 2020 Operating Manual 14 48 44 Test Validation Condition Concentration C1 lt C2 Concentration 1 lt 2 lt ce gt to show list Screen 23 Validation gt Options e Column Base Select the base for the calculation e Column Condition Enter one ore more conditions which must be fulfilled by the entire test to be valid Press lt ce gt to activate the input menu e All special positions defined in the plate layout standards controls blanks e Arithmetic functions nv lt gt e Logical combinations NOT true if condition is not fulfilled AND true if all conditions are fulfilled OR true if one or more conditions are fulfilled XOR true if only one of the conditions is fulfilled e Variables please refer to chapter 5 3 4 Move cursor to select a row and confirm with the corresponding function
45. irection for the replicates is automatically set according to the filling direction of the AG control strips e Auto Replicates automatically set replicates valid for all samples blanks controls and standards on the plate Set the following parameters e Number Number of replicates maximum of 12 replicates for horizontal direction maximum of 8 replicates for vertical direction e Direction Direction horizontal vertical of replicates if AG control strips and replicates are used together the filling direction for the replicates is automatically set according to the filling direction of the AG control strips gt Function keys F4 Back Return to the previous screen F5 Next Proceed to the next screen 5 3 3 Plate Layout 14 22 48 t 12 Well T Test Plate Layou 8 9 16 11 SE om PR D o D D lt enter gt to set well Zeez to clear well Screen 9 Plate Layout 28 70 Anthos 2010 2020 March 2010 Operating Manual anthos 2010 2020 gt Options e Well Type Select one of the possible well types Standard Control Blank Press lt F1 Layout gt to jump to the plate layout position the cursor into the desired well and press lt enter gt to define this well as the selected well type To select a different well type press lt F1 Well Type gt and select with the cursor keys In the well type window the ID number of the standard control etc can be increased by pressi
46. key Delete the last entry with lt ce gt Finally re confirm the formula with lt enter gt gt Function keys F4 Back Return to the previous screen F5 Next Proceed to the next screen 44 70 Anthos 2010 2020 March 2010 Operating Manual anthos 2010 2020 5 3 13 Printing amp Safeguarding the Test in the Test Memory 5 3 13 1 Printing 15 26 82 Test Printout Contents Available Choosen Standard Table Plate View lt a gt lt gt to select Screen 24 Print Options gt Options Select the desired printing options in the Available window and move the into the Chosen window with the function key F2 gt The options can be removed form the Chosen window by selecting and moving the into the Available window with the function key F3 lt gt Function keys F2 gt Move the selected printing option from the Available into the Chosen window F3 lt Move the selected printing option from the Chosen into the Available window F4 Back Return to the previous screen F5 Finish Finish defining or editing the test and proceed to the storage menu 45 70 anthos 2010 2020 Operating Manual 5 3 13 2 Safeguarding the Test in the Test Memory 14 52 46 Test Printout Contents Available Choosen Raw Data Confirm Do you want to store this test Tk ik lt 4 gt lt b gt to select bu
47. leads to the next dialogue element lt ce gt for changing and editing existing values and for activation of the text and or formula input lt display gt holding this key and pressing the cursor up or down keys in or decreases the display contrast 18 70 Anthos 2010 2020 March 2010 Operating Manual anthos 2010 2020 5 1 2 Display Quick Plate Test Setup 10 45 55 AM Main Anthos 2020 Version 1 8 Filters 405nm 450nm 492nm 550nm 620nm Screen 2 Main Menu gt Options Press lt menu gt and use the cursor keys to select menu options gt Function keys F1 QUICK Start a quick measurement without test programming F2 RUN TEST Start a pre programmed test F4 INIT Perform an initialization of the instrument F5 SHUTDOWN Prepares the instrument to be turned off 19 70 anthos 2010 2020 Operating Manual 5 1 3 System Setup Functions Quick Plate Test Setup Plate Test Setup 12 35 50 PM System Setup Time Ei Free Memory 540381 KB Date emm Printer Type BRAG Rs iat X Floppydisk Drive Position Filter Com EM KEN EE ECH ECH ERR CR CR Format hh mm ss seconds may be skipped lt enter gt to leave unchanged Screen 3 System Setup 20 70 In the main menu press lt menu gt and select Setup and System gt Options e Date and time important for printed protocols e Free Memory shows the amou
48. melsammlung fur numerische Mathematik mit Pascal Programmen Bibliographical Institute Mannheim Vienna Zurich Wissenschaftsverlag 1985 p146ff Cubic Spline OD A 0 0001 0 001 0 01 0 1 Drawing 19 Cubic Spline 4 4 Parameter Fit This procedure can only be used to characterize sigmoid curves Minimum number of defined standard points 4 The curve is calculated according to the following formula y Ca ad e DEE 39 70 anthos 2010 2020 Operating Manual Definition The dose level which results in a response exactly half way between the zero dose response a upper asymptote and the infinite dose response d lower asymptote is designated as the midrange of the assay and is designated c In addition the curve is characterized by a slope factor or exponent designated by Di Definition according to Radio Immunoassay and Related Procedures in Medicine International Atomic Energy Agency Vienna 1974 Volume 1 p 165 ff 4 Parameter Fit OD A 0 0001 0 001 0 01 0 1 Drawing 20 4 Parameter Fit 5 3 10 Qualitative Evaluation 14 40 52 Test Qualitative Evaluation Base Group Name 1 Ri Cutoff B eake te Group Name 2 Bilag Interpretation Mode Number of Groups lt ce gt to activate text input Screen 21 Qualitative Evaluation 40 70 Anthos 2010 2020 March 2010 Operating Manual anthos 2010 2020 g
49. mitted characters Only the GET PRINTER STATUS command checks the ACK signal A switched off printer can be the reason for this error sb1 5 AD TIMEOUT ERROR Means that there is no signal from ADC Chip This indicates a hardware error sb1 6 LAMP ERROR The MAX measurement is done with diode values between 60 000 to 65 000 If it is not possible to reach this lamp energy level the LAMP ERROR is reported Possible reasons 1 The lamp lamp connections and or the lamp regulation are defected 2 The transport system is not in the load position 3 The filter wheel is not in the correct position 4 The lamp energy value is not correct an error which may have been coming up during the SET FILTER command or an EEPROM error 61 70 anthos 2010 2020 Operating Manual gt sb1 7 Y SENSOR INTERRUPT ERROR The movement of the transport system is not correct i e the location of the wells is not correctly in the optical path gt sb2 0 MEASUREMENT TIMEOUT ERROR The motor of the transport system does not move or a transport sensor is defected gt sb2 1 Watch Dog ERROR Set by the Watchdog reset routine Indicates an unknown usually undetermined system error gt sb2 2 TO LOW MOTOR SPEED ERROR The transport speed is too slow gt sb2 3 DELTA MAX TO HIGH The lamp energy is not stable gt sb2 4 START POSITION ERROR The transport system is not in the load position The transport INITIALIZE command should
50. ndards Point to point 2 standards Cubic spline 3 standards 4 parameter fit 4 standards Maximum number of standards 12 See chapter 5 3 9 2 below for detailed description of curve fits Scaling Select linear or logarithmic scaling for x axis and y axis If the sequence of standard concentrations is produced by a multiplication factor e g 2 4 8 16 set the x axis logarithmic If the sequence is produced by a more less constant increase e g 2 4 6 8 10 set the x axis to linear For the y axis linear will be the choice in most cases except of extreme dynamic ranges The best scaling setup is the one which produces the most linear curve at medium close to 45 slope Please note that it is not possible to use a log scale on x axis if the first standard has concentration zero for mathematical reasons Minimum Correlation This refers to linear regression only and is a limit for the standards to fit a straight line A good linear curve should be better than 98 0 The best fit is 99 9 Enter a value between 1 and 99 9 on the numerical keyboard and confirm with lt enter gt 37 70 anthos 2010 2020 Operating Manual Extrapolation The concentration of samples having a higher lower measurement result than the highest lowest standard can be determined mathematically A continued calculation of the curve can be performed up to 40 outside the real curve limits This percentage corresponds to the conce
51. ng lt F2 Number gt gt Function keys F1 Layout Well Type By pressing this key the cursor can be set from the Well Type window into the Layout window and vice versa To place the selected well type in the highlighted position of the layout press the enter key F2 Number Press this key to increase the ID number of the selected well type control standard sample F3 Sample Fill Sample Clr Press this key for automatic sample positioning The vacant positions are filled with samples in the prescribed filling direction After filling all positions the function key will change to Sample Clr which allows to clear all samples set previously F4 Back Return to the previous screen F5 Next Proceed to the next screen 29 70 anthos 2010 2020 Operating Manual 5 3 4 Variables For the evaluation of measurement results it is sometimes necessary to use lot dependent factors or concentrations e g lot dependent titers of standards For this reason up to 24 variables per test can be defined and may be used for further calculations Variables Name Value OO oo oc 1 2 H H H H 0 H H H H 0 Screen 10 Variables gt Options e Name Define a name for the desired variable by pressing lt ce gt and enter the name with the cursor keys e Value Enter the value for the selected variable gt Function keys F4 Back Return to the pre
52. nt of free available space on the hard drive or flash disk e Printer Type possible choices for printers are e Matrix 9 pin e Matrix 24 pin e Inkjet Canon amp Epson e Inkjet HP A others e Laser e IBM Proprinter e Postscript e HP Paint jet e HP Think jet e Floppy Disk Drive to activate deactivate press lt ce gt an activated Floppy Disk Drive is marked with X To activate functions regarding to data management using disks restart the machine with activated Floppy Disk Drive e Filter position and wavelength To change delete wrong entry with CE and enter new value using the numerical keyboard Anthos 2010 2020 March 2010 Operating Manual anthos 2010 2020 gt Function keys F1 Print Cfg Print the configuration of the instrument F2 Save F3 am pm Switch between am and pm only when Date Format is set to 12 h mode see below F5 Set Filters After changing one or more filters always perform F5 Set Filters to make sure that the changed filter configuration is stored and all filters will work with the correct lamp energy settings 5 1 4 Setup Menu Options e Floppy disk Maintenance Refer to chapter 5 7 5 e Remote control Instrument can be controlled via PC e Data Transfer Data transfer via ADAP Data storage up and download of tests and plates on instrument or PC e Update For software update of the 2020 e Language You can choose between German and English language e
53. ntration range between the lowest and highest standard 100 Extrapolated values are marked with an X on the printout If a curve loses its uniformity in the extrapolation range it reaches its maximum or minimum extrapolation is blocked Enter a value between 0 and 40 on the numerical keyboard and confirm with lt enter gt 5 3 9 2 Curve Fit Functions Four different mathematical functions are available to calculate the standard curve It is essential to select the best fitting function for the standard points according to the test 1 _Linear regression Construction of a straight line according to the least squares method with the highest possible approximation to all standard points Minimum number of defined standard points 2 Formula y kx d Linear Regression op 0 0001 0 001 0 01 Drawing 17 Linear Regression 2 38 70 Point to point Direct connection of all standard points Minimum number of defined standard points 2 Anthos 2010 2020 March 2010 Operating Manual anthos 2010 2020 Point to Point OD A 05 fi 0 0001 0 001 0 01 Di Drawing 18 Point to Point 3 Cubic spline All standard points are connected by the best fitting curve Can only be used for non linear and non sigmoid functions Minimum number of defined standard points 3 Definition of the natural cubic spline as found in G Engeln Mullges F Reutter For
54. o SClCCN es Ath AAS ae ery E lias cite Ant oe I Ee tie 51 5 7 N WEN RE e E 51 5 7 1 View EE 51 5 7 2 Print Plate sc Jie eases ada easel Ani oe aie el eon a ase 51 5 7 3 Re Evaltiate Plates iesen eiee A E E S aS aa AREEN AE AE EESE A E EEEE geet 51 5 7 4 RE 52 5 7 5 Floppy Disk Data Management 52 6 MaiNtOM AN Ce iiiiiciciscsisiciscuisdcdnccencsiacwiacuiaduiacsdncndacuaacudaadiadudaatiadudacdandciaatancvescwiaaies 55 6 1 ai else Rn 55 6 2 Cleaning and Disinfection EE 55 6 2 1 GIO une DEE 55 6 2 2 DiSinfectiOnisA wAianei ada tie ie Ga ial Aa aa ee ie ares 56 6 3 Changing A E 56 6 4 Filter Ee EE 57 65 Error MESSAGES eEee iy cocaine heck Oia E dav gear E 58 6 5 1 Mathematical Error Messages A 58 6 5 2 Device and Communication Error messages ssssessseesiessiessietriettettnttnnttnnrnnnnnnnt 60 6 6 Software History 2020 Fimware 63 6 6 1 EES IERT E AEE T ETE IE AE ELENEN eee tite in AB ear digs ETE T 63 6 6 2 VOSTON EE 63 6 6 3 VOTON 124 EES 63 6 6 4 KE DEE 63 6 6 5 KEE BO EE 63 6 6 6 KA Ee 220 EE 63 T ee E seccdeeciccctvesssecssecdsecduecenandebodswudessesevdensdeecdecedasedeusdaueduaedsuedsandesudeedeaedarecs 64 7 1 Principles of Photometry Measurement A1 64 7 1 1 Absorbency readies e a a NE ANE EEA 64 7 1 2 Measurement at Specific Wavelengths ccccccceeeeeeeeeeeeeceeeeeeaeseeeeeseaeeesaeeesaaeeenees 65 7 2 Eval ati n applications suit cst cox ndur ine ni a T E AEE AE r demise 66 7 2 1 Quick reference for test programming
55. o change or add a filter by turning the filter wheel Do not leave a filter position empty between filters installed Pull the rubber band carefully and insert exchange a filter Pay attention that the arrow on the painted on the filter frame shows upwards The filter must be clean Avoid dust or fingerprints Reassemble the instrument make sure the internal serial connection is plugged in properly and plug it to the mains Turn the reader ON Select the SETUP F5 then SYSTEM Select the filter position on which you have exchanged added a filter and clear it by pressing the lt CE gt key Enter the new wavelength The filters shown in the menu must be the same as on the filter wheel The number of the filter position 1 to 8 is indicated on the filter wheel Press lt ESC gt and wait for the new initialization 1 min Turn the unit OFF Perform an initialization INIT F4 when turning the instrument ON next time 57 70 anthos 2010 2020 Operating Manual 6 5 Error Messages 6 5 1 Mathematical Error Messages gt A Addition is displayed if the argument left or right of the is missing Argument not in domain of function Undefined result is displayed when an Argument of a formula is out of the defined range of this operation e g Log 1 gt C Calculation of well XNN state window is displayed if the calculation of the transformation is erroneous Correlation too low state window is displayed if the
56. oughly after handling test fluids If equipment has been in contact with hazardous substances it must be disinfected prior to shipment in accordance with the effective provisions Application e Make sure that the plate is inserted correctly with well A1 on the upper left position e Always consider that the reader is an optical instrument and is using the liquid surface and the well bottom as optical surfaces e Avoid foaming and make sure that no bubbles or particles in the well can disturb the measurements Extremely strong reactions may cause substrates to precipitate e Visually inspect the bottom of plates before use for optical distortion and contamination e Use bichromatic reading for eliminating unspecific signals caused by plate material or contamination e When using plate frames for column row strips or single wells breakable strips check that all strips and wells are pushed down completely and are level with the frame 5 70 anthos 2010 2020 Operating Manual e Keep the bottom of the plate clean and dry If liquid has contaminated the well bottoms wipe off with a soft cloth or paper suited for optical surfaces Never touch the optical surfaces with your fingers e Do not insert plates into the reader which are colder than the instrument and environment If this is required by the application check the well bottoms for possible condensation before reading Moving Parts e Do not touch the plate during movement o
57. ower outlet the instrument is connected to have to meet the applicable technical safety requirements If the instrument is turned on using the power switch on the rear side of the device the green power indicator on the left front side of the device has to be illuminated When connecting the instrument with an external computer make sure the instrument is switched off the power indicator is dark If the instrument is turned on turn it off using the power switch on the rear side of the device The power indicator is located on the front left side of the unit and is marked with the following symbol Q The power indicator may show 2 different states Green light Instrument is switched on ready for operation Not illuminated Instrument is not ready for operation 13 70 anthos 2010 2020 Operating Manual Please Note The power indicator does not indicate that the instrument is turned on or off or that it is connected with mains but only that the instrument is ready for operation In order to make sure the instrument is disconnected from power the main switch on the rear side of the unit has to be switched to the OFF position indicated with a 0 symbol Alternatively the power cord can be disconnected from the unit 2 Connecting 2010 2020 with an external computer Turn the instrument off Connect the serial interface 9 pin DB9 connector on the computer with the serial cable supplied by anthos Lock the conne
58. pective error message will be displayed on the screen The plate identification number combination of letters also possible is entered prior to the measurement in the Run test menu 5 7 1 View Plate To re view the measurement results of a certain plate select the plate as described above Press lt menu gt select Plate then View and choose the desired plate Now the result screen is activated For details concerning the measurement results please refer to chapter 5 6 5 7 2 Print Plate Press lt menu gt select Plate and then Print Select the plate to be printed Now all results are printed Make sure a printer is connected and on line and that the correct printer is selected in the system setup 5 7 3 Re Evaluate Plate Press lt menu gt select Plate and then Re Eval Select the plate of which the measurement values shall be re 51 70 anthos 2010 2020 Operating Manual evaluated Select the test with which the plate shall be re evaluated Now the selected plate is re evaluated with the currently selected test For detailed information concerning the results please refer to chapter 5 6 5 7 4 Delete Plate To delete a plate please select this plate as described above Press lt menu gt select Plate and then Delete Either the selected plate Plate or the whole plate storage All Plates can be deleted Re confirm your selection wit
59. port 14 SD En E E 15 3 1 ETE L1 l EE 15 4 ASTANA TM 16 4 1 Un packing and System Set up 16 4 2 OWN Ci a E EE E EE EE ge rar EE EE When eaekedad asta aek etka Baron teats 16 4 3 SoftW re TE EE 17 4 3 1 ADI and ADAP iia cfecs cle E 17 4 4 Switching Be EE 17 5 2020 On Board SOMWALE vciisiicscvevicsciwesinsenicandeiieninsnnnsensaeivieiieiwsnunseiwanenineneninae 18 5 1 Controls and SYSISMV SMUD EE 18 5 1 1 Description of the key functions 00 eee eee eeneee ee enne cece eae ee ee taaeeeeeeaaeeeeeeaaeeeeeeaaeeeeneaeeeeee 18 5 1 2 MIEL 19 5 1 3 System Setup FUNCTIONS AANEREN 20 5 1 4 setup Ment Options a aise eile days i ci Reed NEES 21 5 2 QUICK measurements without test programming and etorage 22 5 2 1 Pip SINGIe e EE 22 5 2 2 FS TEE 23 5 3 Test Definition Programming of tests evaluation modes ssssseeeeeesseseeene 25 5 3 1 Test Identification and Pre Measurement Functions 0 ce eeeteeeeeeeneeeeeenaeeeeeeaeeeeeeaae 25 5 3 2 Plate Layout Patter EE 26 5 3 3 NEIE e cn orange alti tin a nbin Girt ae oats aioe 28 5 3 4 Malables anna aT cd sncndea sede geese seen ee ioe an va sn neea O ii ce seud Ee bie 30 5 3 5 Pre Measurement FUnCtions ccceccccceeeeccceeeeeeceeeeeeeaeeeseaaeeeseaaeeeseeaaeeeseeeaeensneeaeentees 31 5 3 6 Wavelength Selection ccscceccceceeceeeeeceeeeeceeeeeceaeceeaaesgeeeeceaeeesaaeseeaeeseeeesaeseeaaesseneesaas 32 5 3 7 Kinetic Absorbency Measurement ccccceeeeeeeeeeeeeeeeeeeee
60. r of readings 30 up to 40 Interval shaking 3 5 seconds medium speed Data reduction Maximum slope Most probably the total kinetic measurement time can be set shorter than the normal substrate incubation time TMB measured in the blue phase at 620nm has about one third of the optical density than the same concentration measured after stopping in the yellow phase Samples and standards which would reach overflow in an endpoint reading will remain within the good measurement range during kinetic readings Therefore the dynamic range and accuracy of ELISA s may be significantly improved with kinetic readings A main advantage of kinetic readings is the elimination of the critical timing between adding of substrate and stopping solution for all wells Anthos 2010 2020 March 2010
61. rds Standard Points S1 S2 S3 S4 S5 Concentration 0 1 1 10 100 1000 Values Response V1 C1 V3 V2 C1 V3 V3 C1 V3 V4 C1 V3 V5 C1 V3 Formulas If the actual measurement value of the standard 3 C1 deviates from the original measurement value V3 all standards are re adjusted by the correction factor C1 V3 corrected Original Curve OD Ist new curve 2nd new curve 0 000001 0 0001 0 001 0 01 0 1 Fig 5 Standard Curve Graph Kinetic reading for extended range ELISA s With photometry endpoint readings the logarithmic scale of optical density may be a limiting factor for some ELISA applications Quantification of TSH for instance would benefit from a broader dynamic range than normally available If the substrate is TMB kinetic reading can extend the measurement range of the assay The result of the kinetic data reduction e g mOD min may be used as the basis for any further quantitative and or qualitative evaluation The maximum slope maximum reaction speed of the color development at 620 nm corresponds to the amount of bound enzyme In fact stopping of the color reaction means setting of an artificial endpoint and uses also the different reaction speed of different enzyme concentrations 69 70 anthos 2010 2020 Operating Manual 70 70 Example of Test Parameters Measurement filter 620 nm Interval time 20 seconds Numbe
62. re no fixed positions used for calculations The software will find the right wells according to the set names in the plate layout The mean value is calculated automatically for all replicates of a blank control standard or sample 7 2 1 5 Blank Subtraction The mean of positions marked as B blank is subtracted automatically from all other wells If a blank should not be subtracted it has to be named as a control 7 2 1 6 Single Point Calibration Index Calculation B Bo Calculation The Transformation Formula allows calculating a ratio to a particular control for all other controls standards and samples A common application is the calculation of the percentage of binding with competitive assays eg X X C1 100 where C1 is the maximum binding control With the use of additional concentration factors and correction factors this formula provides also single point calibration and cut off ratios On the printout the result of this formula is reported with its given name 66 70 Anthos 2010 2020 March 2010 Operating Manual anthos 2010 2020 7 2 1 7 Use of Variables Lot dependent values such as calibrator concentrations or correction factors may be programmed as variables Enter default values for the number of variables required During programming you can use the names V1 V2 which refer to the list of defined variables At the beginning of each measurement the variables are displayed for review At this time permanent
63. t Options Base Select the base values original measurement values or results of the relative calculation by selecting the Result Name or concentrations for the qualitative evaluation If quantitative and qualitative evaluation is combined the measurement data can also be displayed in concentrations and evaluated qualitatively as such by using the standard curve Interpretation Mode Set the type of threshold value subdivisions 1 Normal Up to four thresholds cutoffs can be programmed individually and up to 5 groups selected 2 Auto ranging Upper and lower threshold are programmed The intermediate range can be divided into 2 to 10 equidistant groups 3 ranging Upper and lower limits are programmed as cutoffs The sample measurement values are printed also as percentages on the printout the sample measurement values are scaled in percent Number of Groups Only appears for Interpretation Mode normal Enter the number of groups to be subdivided minimum 2 maximum 5 Number of Divisions Only appears for Interpretation Mode Auto ranging Enter the number of equidistant tolerance value subdivisions minimum 2 maximum 10 5 3 10 1 Cutoffs and Ranges Cutoff values CV Cutoff values for the results concentration values relational values Enter the formula or constants for the programmed cutoff values normal up to 4 cutoffs can be programmed auto ranging or ranging two cutoffs must be programm
64. t represents 100 binding has to be set in relation to all samples of the plate in order to deduce their amount of binding X X NC1 100 where X is the re calculated value for all samples X is not displayed on the screen Now the relational values correspond to percentage values showing the binding degree of each samples gt Additional mathematical functions The software includes the following 4 additional mathematical functions to be used in formula definition e Pow x y xy e Log x log X e Exp x CN e Ln x In x 35 70 anthos 2010 2020 Operating Manual gt Function keys F4 Back Return to the previous screen F5 Next Proceed to the next screen 5 3 9 Quantitative Evaluation This method obtains sample concentrations using a standard curve 14 37 21 Test Quantitative Evaluation Base IER Units of concentr Standard Points Nr Concentr x Response Value y Curve fit method 1 X axis scale Y axis scale OWA OO E GA M Min Correlation EE Extrapolation EH Screen 16 Quantitative Evaluation 5 3 9 1 Set up a Standard Curve gt Options e Base Select the calculation base y values for the concentration calculation If a Relative Calculation formula is set up the original measurement values default or transformed values results of the relative calculation may be selected The name for transformed values refers to the Result
65. t screen 33 70 anthos 2010 2020 Operating Manual 5 3 8 Relative Calculation 14 31 52 Test Relative Calculation Result Name W3aw E lt ce gt to activate formula input a Wm Screen 14 Relative Calculation gt Options e Result Name Activate the text input with lt ce gt and enter a result name e Formula Setting all plate positions in relation to a predefined value This value is calculated according to an algebraic formula which may contain all the standards controls and blanks defined in the plate layout as well as the fundamental operations of arithmetic and numerical constants and the previously defined variables Press lt ce gt to activate the formula input 34 70 Anthos 2010 2020 March 2010 Operating Manual anthos 2010 2020 14 34 29 Test Relative Calculation Result Name W aw x E Formula Input X C1 100 d G Pow Log Ln BL C1 C2 Exp 1 2 H Screen 15 Formula Input The display menu shows all the standards controls blanks and variables defined in the plate layout and the pre measurement functions as well as a variable X This variable X represents all the original measurement values It must appear exactly once in each algebraic formula Move the cursor to select a row in the screen display and select the desired character with the corresponding function key Numbers may be entered on the numerical keyboard Example A negative control NC1 tha
66. tching on Operating voltage 90V 130V 180V 250V auto sensing Fuses user exchangeable 2 pcs 1A TH250VAC slow blow Built in Fuse on power supply 1 pcs 2A TH250VAC fast blow Ambient temperature 15 C 40 C operation 25 C 50 C storage e Relative humidity 15 85 non condensing operation lt 95 non condensing storage e Air pressure tolerance 54 000 106 000 Pascal e Height over sea level operation up to 2000 m e Installation Category II e Pollution Degree 2 2 2 5 Limits of error and tolerance limits Wavelength instability lt 2 nm filter Spectral width at half maximum intensity lt 12 nm at gt 450 nm lt 15 nm at lt 450 nm Integral portion of misradiation measured at all installed filter with band stop filter Result is outside of measurement range Linearity measuring device Grey Steps lt t 0 75 and 0 005 O D From 0 1 O D to 2 50 D Wavelength Range 400 750 nm Resolution 0 001 O D Indication Range 0 000 3 300 O D Accuracy lt 2 at 1 O D and 0 005 OD Measured with 450 or 492 620 405nm Short time reproducibility lt 0 3 at 1 O D Reading Speed single wavelength 45 sec dual wavelength 90 sec Measurement range 0 000 2 500 O D The tolerances of the measuring devices are based on the manufacturer s information 9 70 anthos 2010 2020 Operating Manual 2 3 General technical data 2 3 1 2 3 2 2 3 3 2 3 4 2 3 5 10 70
67. tton lt enter gt to confirm Screen 25 Safeguarding a test gt Options Select the options with the cursor keys and confirm the selection with lt enter gt e Yes Exit the define edit mode and store the test in the test memory of the instrument e No Exit the define edit mode without storing the test All definitions changes will be discarded e Cancel Return to the define edit mode Finish screen 5 4 Editing and Clearing of Defined Tests 5 4 1 Edit Test Changing a Test Already Programmed Press lt menu gt and select the test menu Choose the Edit option Select the test which shall be edited in the list and confirm with lt ENTER gt Now all parameters of this test can be edited please refer to chapter 5 3 for the descriptions of the parameters 46 70 Anthos 2010 2020 March 2010 Operating Manual anthos 2010 2020 5 4 1 1 Copy a Test Follow the steps described above chapter 5 4 1 Change the test name in the first screen make changes to the parameters if necessary and store the test The test will be stored with the new name No changes are made to the original test 5 4 1 2 Delete Test Press lt menu gt and select the test menu Choose the lt Delete gt option Select whether a single test or the whole test memory shall be deleted e Test Select the test to be deleted and confirm the selection e All tests Confirm that all tests in the memory shall be deleted 5 5 Run Test
68. vious screen F5 Next Proceed to the next screen 30 70 Anthos 2010 2020 March 2010 Operating Manual anthos 2010 2020 5 3 5 Pre Measurement Functions 16 11 59 Test Premeasurement Functions Waiting Duration E sec Screen 11 Pre measurement Functions gt Options e Waiting Duration Enter the waiting time waiting prior to measurement in seconds 1 999 gt Function keys F4 Back Return to the previous screen F5 Next Proceed to the next screen 31 70 anthos 2010 2020 Operating Manual 5 3 6 Wavelength Selection 16 14 22 Test Single Point Photo Meas Filter nm Ref Filter nm M lt 4 gt lt b gt to select Screen 12 Single Point Photo gt Options Please refer to chapter 5 2 1 F1 Single Point for detailed information on all settings gt Function keys F4 Back Return to the previous screen F5 Next Proceed to the next screen 32 70 Anthos 2010 2020 March 2010 Operating Manual anthos 2010 2020 5 3 7 Kinetic Absorbency Measurement T Quick Plate Test Setup 13 18 52 Test Kinetic Photo Meas Filter nm ECE Ref Filter nm RER Interval sec Data Reduction Number Of Cycles lt 4 gt lt h gt to select Screen 13 Kinetic Photo gt Options Please refer to chapter 5 2 2 for detailed information on all settings gt Function keys F4 Back Return to the previous screen F5 Next Proceed to the nex
69. xternal computer 6 2 Cleaning and Disinfection All parts of the reader that come into contact with potentially infectious material must be treated as potentially infectious areas Authorized trained personnel in a well ventilated room while wearing disposable gloves protective glasses and clothing should perform the cleaning and disinfection procedures The following materials are recommended for cleaning and disinfection of the reader e Non lint tissues e Protein degrading mild detergent e g Mucasol Rogypon or saline solution 0 9 NaCl e Ethanol 70 or chloric solution 1500 ppm Never spray directly into the interior of the reader 6 2 1 Cleaning Clean the reader regularly and immediately after spillage This has to be done with due care and attention Always observe laboratory safety rules and regulations Do not use force when cleaning the reader e Wipe off spills immediately with soft tissue e Avoid build up of dust on the instrument and wipe off visible dust e Cover the instrument with the dust cover if not in use 55 70 anthos 2010 2020 6 2 2 Operating Manual The following cleaning procedure should be performed on demand 1 2 Switch off the reader Carefully wipe off the entire reader with non lint tissues that have been moistened in a protein degrading mild detergent or a saline solution Carefully wipe off the entire reader with non lint tissues that have been moist

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