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TeSeE NSP Operator`s Manual Ver. 1.16 - Bio-Rad

1. 75 User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 16 2 Reagent reservoirs cleaning procedure 1 Precaution before use e Please use only reservoirs and associated covers adapted to the NSP system e Please use reservoirs and associated covers perfectly cleaned and dried e Please check that reservoirs and associated covers are correctly set up on the instrument position direction dip 2 Cleaning procedure for reagent reservoirs and associated covers buffer A PK and B Every day during daily maintenance procedure e Rinse with distilled water the two reservoirs and associated covers e Rinse with ethanol e Dry on paper towel Never let residual volume of reagents that could dry in the reservoirs 3 Decontamination procedure for the waste reservoir and associated cover Only if the waste reservoir has been contaminated e Soak the tray and its cover in 1N sodium hydroxide or in 6 CHL hypochlorite sodium during 1 hour minimum e Rinse with distilled water to eliminate any traces and or vapours of the deco
2. 2 If you try to close the NSP application software without carrying out daily maintenance a message will be displayed with a warning sound Operator will be then invited to perform the Daily Maintenance INFORMATION Execution of daily maintenance can be postponed However please be sure to carry out daily maintenance at the end of the day s operation Pr Maintenance Daily Maintenance Daily Maintenance Remove tip rack from the table Remove buffer reservoir from the table Wash buffer reservoir Remove waste reservoir from the table Wash waste reservoir Remove sample plate from the table Remove purification plate from the table Remove tip bag from the waste tip drawer Dane Remove tip racks from stackers Clean up the work table Cancel il Fig 10 1 1 Daily Maintenance screen 48 User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 10 1 1 Daily maintenance operations A CAUTION Be sure to wear gloves when carrying out maintenance After maintenance dispose of gloves and any pieces of cloth used The following operations are carried out during daily maintenance h Tip rack is removed from the table NO Buffer A PK reservoir is removed from the table CO Buffer A PK reservoir is washed Eh Buffer B reservoir is
3. 2 Inthe Plate selection screen click the plate to be processed then click Select button The Reagent batch selection screen will be displayed Fig 8 2 1 8 2 Reagent Batch selection amp Run Start Reagent Batch Selection all E3 Reagent batch selection Reagent Batches Expiration dates Select New Cancel Batch ID 000001 Fig 8 2 1 Reagent Batch selection Note Click on the corresponding column header to sort the batches by batch ID or expiration dates From this point you can also create a new reagent batch Click New in the reagent batch selection screen fig 8 2 1 then follow instructions on chapter 6 1 point 2 1 In the Reagent batch selection screen select the reagent batch to be used then click Select The Reagent Disposable tips inventory screen will be displayed Fig 8 3 1 8 3 Reagent Disposable tips inventory 2 Run Start Reagent Disposable tips inventory Reagent Disposable tips inventory Reagents Confirm Description Required Buffer 4 PK 12000uL m i Buffer B 12000uL Disposable Tips Detected Resut Disposable Tips Left 160Tips 2Racks 384Tips 4Racks OK Empty Racks Area Right 2Racks SRacks OK Cancel Plates Detected Resut Sample Plate ID PO20000006040 PO20000006040 OK Purification Plate ID PO20000006001 OK Waste Bag Detected Result Waste Tips 160Tips 392Tips OK
4. Fig 10 2 3 4 Placing bigger O ring Fig 10 2 3 5 O rings correctly placed 5 Repeat step 3 and 4 and place O rings on all nozzles 6 Close the unit cover 52 User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 10 3 Monthly Maintenance Monthly maintenance is maintenance that is carried out once a month One month after beginning use of the N S P system the first day for monthly maintenance will arrive Monthly maintenance menu can be accessed in either of the following two ways 1 Select Monthly from Maintenance in the menu bar Fig 10 3 1 2 On the day that monthly maintenance is carried out if the NSP application software is closed a warning message will appear Operator will be then invited to perform the Monthly Maintenance amp Maintenance Monthly Maintenance ES KA Monthly Maintenance M Weekly Maintenance Bone Check temperature of the incubator FT Liquid Sensor Check Cancel Fig 10 3 1 Monthly Maintenance screen 10 3 1 Monthly maintenance operations 1 Daily maintenance is carried out Refer to 10 1 Daily maintenance 2 Weekly maintenance is carried out Refer to 10 2 Weekly maintenance 3 4 4 Incubator temperature is checked Liquid sensors are checked 10 3 2 Monthly maintenance
5. Fig 8 3 1 Reagent Disposable tips inventory 36 User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 1 Use the onscreen display to confirm reagents tip number and status of stackers and tip disposal box and if necessary carry out any necessary preparations or adjustment a The required column indicates the necessary volume of reagents and number of disposables that will be needed to perform the run b The detected column indicates the number of disposables detected on the NSP sysiem c Click Check button Refer to 7 Operation setup to control the number of disposables and plates Ids loaded on the NSP system Note If covers or waste tip drawer are opened Confirm button will not be selectable anymore Check button must be clicked again to access Confirm 2 When all preparations are finished click the Confirm button The Protocol check screen will be displayed Fig 8 4 1 8 4 Operation start amp Run Start Protocol Check J x Protocol Check Sample Plate ID po2z0000006040 Purification Plate ID JPozoo00006001 O Batch ID foo0001 sits Slaughterhouse 1 Slaughterhouse A Operator ichiro M Sample Transfer M Pre Incubation M Buffer APE M Incubation Delay Iw Buffer B M Last Heat
6. Red icon System not operable when Temperature gt 39 C temperature of incubator is higher than 39 C Communication icon Communication status between NSP and PC Description Green icon Communication with NSP is maintained Communication with NSP is not active Red icon 4 4 Closing the NSP application software Application software is closed by clicking on X on the top right of the main screen A confirmation message will appear Before to exit the NSP application software please do not forget to perform the maintenance procedures see chapter 10 If not the software will ask you to perform the maintenance procedures before to leave sG User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 5 Plates menu Select Plates from the Main menu to open the Plates menu 5 N 5 P New Sample Pripjeparator Run Plates Batches Search Maintenance eu Edit Delete Fig 5 1 Plates menu 5 1 Registration of a new sample plate New 1 Select New from the Plates menu Fig 5 1 1 amp 4 S P New Sample Pripleparator Ruin Plates Batches Search Maintenance Fig 5 1 1 New sample plate 2 The New Plate Registration screen is displayed Fig 5 1 2 Make sure that the cursor is in the Plate ID box and u
7. Yes LI No Search III Yes Yes Maintenance S S y Daily Il Yes _ Yes Weekly Il Yes IL Yes Monthy Il Yes TI Yes Repot Yes TI Yes ps ily Ae Yes pd Weekly Yes IL Yes pf Monthly Yes Yes Serice No No Configuration S S O Communication les No Slaughterhouse Yes No Password J Yes Y UserLit II Yes TI No Evet Il Yes Mo Eventlog Yes IL Yes pC Error History Yes Yes Temperature Lol No Liquid Check II Yes No Hein Ves Yes 70 User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 14 Error messages When a problem has occurred on the N S P system an error message will be displayed on the computer screen After confirming the error content click OK on the Error message screen and either restart or stop operation Some error occurrences do not necessitate the stoppage of operation The error will be recorded and can be confirmed on the results report after operation is finished Error history can also be referred to 14 1 Error message screen Error number error name and error content can be checked on the Error message screen After checking click on OK 14 2 Error contents cause Refer to the following chart for details concerning error content No Error Descriptions Treatment
8. User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 8 Operation Run Operation is started once preparations are complete INFORMATON Please refer to 7 Operation procedure for details concerning operation preparation 1 Click Run in the menu bar to open the Run menu Fig 8 1 iB ONS P New Sample Pripheparator Bun Plates Batches Search Maintenance BookRun F4 Fig 8 1 Run Start 8 1 Sample plate selection 1 Select Start from Run Fig 8 1 in the main menu to open the Plate selection screen Fig 8 1 1 2 Run Start Plate Selection Plate selection Select PO20000006040 1 Slaughterhouse 4 2002 09 27 20 00 MANUAL Registered Cancel Plate ID I Po20000006040 Fig 8 1 2 Sample Plate selection Note Click on the corresponding column header to sort the plates by plate ID slaughterhouse ID date time mode or status From this point you can also create a new sample plate Click New in the plate selection screen fig 8 1 1 then follow instructions on chapter 5 1 point 2 35 User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130
9. OOO OOO 0000000000 0000000000 Q 0000000000 OO OQOOOQOOOOOOOOO ok OQQOUQOQOOOOOOO OCOOCOOOOOOODOO O AN KN N LY a KN O O 0 Buffer A PK Reservoir Tip Shooter Incubator Buffer B Reservoir Heating Block 1 4 Containers 96 Tip Rack ref 90 131 Ref 90 131 10 racks of 96 disposables tips KE User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 Deepwell Plate ref 90 132 Ref 90 132 50 deepwell plates Reagent Waste Reservoirs ref 90 158 Reagent Reservoir Waste Reservoir Ref 90 158 NSP reagent waste reservoirs set includes buffer A PK reservoir buffer B reservoir waste reservoir User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 Buffer Waste Reservoirs Heating Block User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 2 Protocol The procedure hereafter describes the purification protocol steps with TeSeE NSP system and DW AO lt is accepted that samples have been previously collecte
10. screen Fig 11 4 2 1 User Delete Fa K Are YOU sure E cms Fig 11 4 2 1 User delete 4 Click OK to close the User List screen Main menu will be displayed 64 User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 11 43 Modification of User property 1 From the User List screen select intended user then click Property button Note Manager level is requested to change the properties of one user in the list 2 Enter user name and password Fig 11 4 1 1 The User Property screen comes up Fig 11 4 3 1 S Congiguration Manager User List User Property User Property Wiser Namely User B Description D User B User Levell Li User Enter within 5 characters New Password eee Ok Cantirmation c EE Cancel Fig 11 4 3 1 User property 3 Change property then click OK 4 Click OK to close the User List screen Main menu will be displayed 11 5 Events 11 5 1 Error history The error history option gives the list and reasons for non terminated operations Run 1 Click Event gt Error History The Error History screen comes up Fig 11 5 1 1 Error History Error History 2002 09 27 20 PO2Z0000006040 Stacker 2 Limit sensor Timeout 2002 09 27 21 PO2000000
11. 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 10 5 Service Restricted to Bio Rad Service Engineer 58 User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 11 Configuration menu Configuration Select Configuration from the Main menu to open the Configuration menu HB N S P New Sample Pr p eparator Run Plates Batches Search Maintenance Configuration Help Communication Slaughterhouse Password User List Event Temperature Liquid Check Fig 11 1 Configuration menu 11 1 Communication port Communication port of the PC to connect the N S P system can be changed by following the procedure below Note Manger level is requested to modify the communication port between NSP and PC 1 Click on Communication in the Configuration menu 2 Enter your User Name and Password Fig 11 1 1 then click OK to confirm E Enter Your Password Enter Your Password User Name Ok Password Cancel Fig 11 1 1 Password entry 3 Select intended communication port Fig 11 1 2 D Configuration Communication Communication Port coms OK Cancel Fig 11 1 2 Communication port selection 59 User manual reference number 90362 Last modification Derni re m
12. 3 Hirotaka Manager Delete 4 User User Property OK Cancel Fig 11 4 1 User list 63 User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 11 4 1 Addition of one User 1 From the User List screen click Add button Note Manager level is requested to add a new user in the list 2 Enter user name and password Fig 11 4 1 1 then click OK to confirm amp Enter Your Password f x Enter Your Password User Name OK Password Cancel Fig 11 4 1 1 Password entry 3 The Add User screen comes up Fig 11 4 1 2 Configuration Manager User List Add User X Add User Enter New User s infomation User Mameli Description Di User LevelfLi User Enter within 5 characters Hew Password Hhi OK Contirmation c Cancel Fig 11 4 1 2 Add user screen 4 Enter required information then click OK 5 Click OK to close the User List screen Main menu will be displayed 11 4 2 Deletion of one User 1 From the User List screen select intended user then click Delete button Note Manager level is requested to add a new user in the list 2 Enter user name and password Fig 11 4 1 1 then click OK to confirm 3 Click OK on the User Delete
13. HALT No response from collection stacker lower sensor SC User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 No Error Descriptions Treatment Note 23 Stacker 2 Failure to collect TipRack HALT TipRack collection failure by collection stacker Incubator Over Heat HALT Incubator temp is above 39 C Incubator Over Cool HALT Incubator temp is below 35 C Liquid Cover Home sensor Timeout HALT No response from Liquid Cover rear sensor Liquid Cover Limit sensor Timeout HALT No response from Liquid Cover front sensor Unit Cover open HALT Cover opened during Protocol operation Continue Operation can be continued if Unit cover open occur during Incubation period 29 Buffer A PK Reservoir set Error Pause Reservoir is not mounted 30 Waste Reservoir set Error Pause Waste tray is not mounted 31 Sample Plate set Error Pause Sample plate is not mounted 32 Purification Plate set Error Pause Purification plate is not mounted 33 Buffer A PK short volume Error Continue Insufficient Buffer A PK liquid volume 34 Buffer B short volume Error Continue Insufficient Buffer B liquid volume 35 Tip set Error Continue Nozzle head failed to load tip 36 Purification Plate short volume Error Continue Insufficient liquid volume in Purification Plate Purification Pla
14. N 10 11 2002 15 45 2 NICO o N N N N N N N N N 10 11 2002 15 46 2 NICO o N N N N N N N N N 10 11 2002 15 50 2 NICO o N N N N N N N N N 10 11 2002 16 24 2 NICO o N N N N N N N N N 10 11 2002 16 24 2 NICO o N N N N N N N N N 10 11 2002 16 25 2 NICO o N N N N N N N N N 10 11 2002 18 29 2 NICO o N N N N N N N N N 10 11 2002 18 29 1 GILLES o N N N N N N N N N 10 11 2002 18 43 1 GILLES o N N N N N N N N N 10 14 2002 10 58 2 NICO 1 N N N N N N N N N 10 14 2002 11 38 2 NICO 1 Y Y Y Y Y Y Y Y Y 10 15 2002 10 20 2 NICO o N N N N N N N N N 10 15 2002 10 21 2 NICO o N N N N N N N N N 10 15 2002 10 21 2 NICO o N N N N N N N N N 10 15 2002 10 21 O Service D N N N N N N N N N 10 15 2002 10 35 O Service D N N N N N N N N N 10 15 2002 10 42 O Service O N N N N N N N N N 10 15 2002 11 03 O Service O N N N N N N N N N 10 15 2002 11 03 O Service D N N N N N N N N N 10 15 2002 11 23 O Service O N N N N N N N N N 10 15 2002 11 53 O Service O N N N N N N N N N 10 15 2002 11 54 O Service 1 N N N N N N N N N nas 2nn2 12 03 n Servire n N N N N N N N N N 2 gt amp 27c Ap 14 16 start 4 E CH ONSE System Control 50 Z N S P New Sample Pr CMAQ 14 16 Fig 10 4 1 1 Daily maintenance report The following information can be accessed from the Daily maintenance report screen Date date the daily maintenance menu was opened User ID amp User name Id and name of the log in operator Status status of the daily maintena
15. Note 1 Unit Communication Error HALT Communication fault of N S P system and PC Barcode Reader Communication Error HALT Communication fault of Barcode Reader and PC 3 Incubator Communication Error HALT Communication fault of incubator and PC 4 I O Communication Error HALT Communication fault of EXDIO I O board and PC X Axis Home sensor Timeout HALT No response from X Axis left sensor K X Axis Home sensor Hit Error HALT Unexpected response from X Axis left sensor X Axis Limit sensor Timeout HALT No response from X Axis right sensor El X Axis Limit sensor Hit Error HALT Unexpected response from X Axis right sensor 9 ZAxis Home sensor Timeout HALT No response from Z Axis left sensor hal Z Axis Home sensor Hit Error HALT Unexpected response from Z Axis left sensor 11 Z Axis Limit sensor Timeout HALT No response from Z Axis right sensor 10 11 12 Z Axis Limit sensor Hit Error HALT Unexpected response from Z Axis right sensor 13 P Axis Home sensor Timeout HALT No response from P Axis left sensor P Axis Home sensor Hit Error HALT Unexpected response from P Axis left sensor P Axis Limit sensor Timeout HALT No response from P Axis right sensor stacker Stacker 1 TipRack Empty Insufficient TipRack in supply stacker 21 Stacker 2 Home sensor Timeout HALT No response from collection stacker upper sensor 22 Stacker 2 Limit sensor Timeout
16. This mark indicates content that if improperly handled may cause death or serious injury This mark indicates content that if improperly handled may cause bodily injury or physical damage to the instrument This mark indicates procedures that need to be carried out with care in order to ensure efficient use of the instrument The Z symbol indicates content that must be handled with care The symbol indicates actions that are prohibited Details of what is prohibited are shown inside the symbol or near it The symbol at the left indicates that the power outlet must not be touched with wet The symbol emphasizes or specifies actions Specified content is shown inside the mark or near it The symbol at the left indicates that the power cord must be disconnected from the power outlet Last modification Derni re modification February 2004 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 WARNING Category Description S Set up the instrument on a leveled surface Placing the instrument on an inclined surface may cause improper operation of the unit resulting in possible accident If a foreign object or liquid gets inside the instrument this may cause fire electric shock or breakdown If water gets inside the unit disconnect the power plug from the power outlet immediately and contact your Bio Rad representative The inside of this unit includes high voltage areas Touching
17. When all information have been entered click Save button to save all data The list of slaughterhouses is indicated including the new slaughterhouse 5 Click OK to close the Slaughterhouse screen Main menu will be displayed 11 2 2 Deletion of one slaughterhouse 1 From the Slaughterhouse screen Fig 11 2 1 select intended slaughterhouse then click Delete button Note Manager level is requested to delete a Slaughterhouse Only slaughterhouses that were never used can be deleted 2 Enter your user name and password Fig 11 2 1 1 then click OK to confirm 3 Click OK on the Slaughterhouse Delete screen Fig 11 2 2 1 The list of remaining slaughterhouses Is indicated gt lauehterhouse Delete xj d Ore you sure Y E Cancel Fig 11 2 2 1 Slaughterhouse delete 4 Click OK to close the Slaughterhouse screen Main menu will be displayed 61 User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 11 2 3 Modification of one slaughterhouse property 1 From the Slaughterhouse screen select intended slaughterhouse and click Property button Note Manager level is requested to change the properties of one slaughterhouse 2 Enter your user name and password Fig 11 2 1 1 then click O
18. displayed 14 User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 4 3 2 Main screen start up After the password is entered click on OK to display the Main screen Fig 4 3 2 1 4B N S P New Sample Pr pleparator Run Plates Batches Search Maintenance Configuration Help Fig 4 3 2 1 Main screen Main function description Function BootRun Initialize motors and sensors Plates Register new sample plate in the N S P software Edit Modify sample plate Delete one sample plate Batches New Register new reagent batch in the N S P software Edit Edit Modify reagent batch Delete Delete one reagent batch Search Search for plates corresponding to specified criteria Maintenance pe ooo Configuration Help Help User manual About N S P Version number and notification 15 User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 Icons Temperature icon Temperature of the incubator must be in the range of 37 C 2 C Icon Status Description Green icon System operable 35 C lt temperature lt 39 C Blue icon System not operable when Temperature lt 35 C temperature of incubator is below 35 C
19. modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 7 4 Waste tip drawer The amount of used tips currently in the waste tip drawer is displayed in the Waste bag column on the Reagent Disposable tips inventory screen When you want to dispose of the tips open the waste tip drawer remove the waste tip container and replace with a new waste tip container After replacement be sure to click on Yes while the software is indicating Did you empty the waste tip drawer in the reagent and tip inventory screen The waste tip counter will be reset A WARNING Be sure not to touch used tips with your hands as there is possible risks of contamination from used tips Be sure to wear gloves when disposing of tips and remove and replace the whole waste container with a new container avoiding touching tips directly 1 Place the waste tip container in the Waste tip drawer 2 Set the Waste tip drawer in the N S P system Fig 7 4 1 Fig 7 4 1 Waste tip drawer 7 5 Unit covers When all preparations are complete close the left and right unit covers CAUTION e The unit will not operate when the covers are open e During operation never open covers except when otherwise indicated Operation will stop if the cover is opened and resumption of operation will not be possible 34
20. operation can be carried out Main access rights are as follows 1 Sample plate registration 2 3 Reagent batch registration 4 Operation data review Unit operation 5 6 Daily weekly and monthly maintenance Revision of personal password 13 2 Manager level The manager level is higher than the User level In addition to the access available to regular users main access rights are as follows 1 Deletion of unprocessed plates that have been registered 2 Deletion of unused reagent batches that have been registered 3 Addition deletion or modification of one slaughterhouse 4 5 Activation or deactivation of the liquid check Addition deletion or modification of a user 13 3 Service Functions that Bio Rad service personnel have access rights to The main functions that can be accessed are as follows 1 Mechanical settings 2 3 Change of storage location of data base file 4 Maintenance program execution Change of incubator temperature setting 69 User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 13 4 User level and access rights list Function Manager User AAA Ke W BootRun II Yes _ Yes Plates SS E MEC EEN New o Yes _ Yes Edy II Yes Ve Delete LI Yes TI Mo Batches S S New LL Yes Les Edy II o Yes TI Yes Delete
21. procedure 1 Daily maintenance Refer to 10 1 1 Daily maintenance operation 2 Weekly maintenance Refer to 10 2 1 Weekly maintenance operation 3 Incubator temperature check 1 Using a pipette dispense 500 ul of distilled water stored at room temperature into all the wells of a deepwell plate then place in the purification plate position N Wait 10 minutes until the distilled waters temperature rises Place thermometers in the E7 wait for 3 minutes then check the temperature Sp 4 lf the water temperature of all wells is between 35 to 39 degrees incubator temperature is normal 5 If the water temperature is outside the 35 to 39 degree range inspection by a service engineer will be necessary In this case please contact Bio Rad 4 Liquid sensor check 1 Install a tip rack in the supply stacker 2 Click on the Liquid Sensor Check box 53 User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 3 As requested pour precisely 30 ml of distilled water into the waste reservoir and place the reservoir in the buffer A PK position Fig 10 3 2 1 Error Message Fig 10 3 2 1 Liquid sensor check procedure 4 The liquid surface is sensed Allowance 28mL lt Detected Volume lt 32mL Volume Detected 30274 ul Fig 10 3 2 2 Liquid sensor check resu
22. reference number 90362 Version 1 17 1 1 Last modification Derni re modification February 2004 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 9 3 2 Plate report printout To print out the operation results of the entire plate click on Complete located on the bottom left side of the Plate view screen Click Print to display the report then click on the top left printing icon to print it The following is an example of a printed plate report 45 User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 User manual reference number 90362 Version 1 17 Bio Rad Operator manual Manuel utilisateur New Sample Pr p eparator Plate Report Sample Plate ID Purification Plate ID Sample Plate Status Date Of Operation Operation Result No of Sample on the Plate Operator Name Slaughterhouse Name N S P Operation Started at N S P Protocol Completed at N S P Operation Completed at Purification Kit Batch ID Buffer A Batch ID PK Batch ID Buffer B Batch ID Liquid Check Temperature of Incubator When Operation Started Incubator Highest Temperature Incubator Lowest Temperature Incubator Result Cover Opened at Cover Closed at Buffer A PK Added at Buffer B Added at Tip Rack Added at Protocol Step 46 All steps preformed N S P System
23. ring ccccccece cece cece eee eeeeeeeeeeae tees tees easeeeaeegnenanes 51 10 3 Monthly maintenance EE 53 10 3 1 Monthly maintenance Operations cccccceeeee cence cece ee eeseeeeeeeeeneeeeaeeeeaneeenees 53 10 3 2 Monthly maintenance procedure US 53 104 lee HEEN ne nsc ee eege taupe EEN 55 10 4 1 Daily maintenance report s nn tte gege eebe SE ERER AE d M n ina 55 10 4 2 Weekly maintenance report 56 10 4 3 Monthly maintenance report 57 10 0 GENICO ee 58 11 Configuration menu Configuration mecenas is 59 Tii COMMUNICAUON POUM GE 59 2 SlaugnterhousE sessions 60 11 2 1 Addition of a new slaughterhouse cccc ccc eee cece cece eee e eee eeaeeeaeeeaeeeaeeeaeenes 60 11 2 2 Deletion of one slaughterhouse cc ccc ese e cece ee eeeeeeeeeeeeseeeeesseeeeseesaneeenees 61 11 2 3 Modification of one slaughterhouse property 62 Ago Es WEE 63 E MISE eet 63 TAA Addition e One E 64 11 4 2 Deletion of one user see 64 11 4 3 Modification of user property ss 65 ES EE 65 Sl EROSI N snauccemaceoarostanaguctayiocnsa pecadagataan oan E eeheen 65 oe EVON e EE 66 11 6 EE leede E 66 ke Ke e EE 67 A A EE 67 PA 68 12 1 Opperator s manual Help 68 12 2 About N S P system control software About NSP oooccocccoccccccconcconcconoconncnnnnnnnnnnns 68 VI User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System
24. searching by period the operator name or slaughterhouse name can also be input to help specify search conditions 9 3 Data report printout 9 3 1 Sample report printout To print out the operation results of a sample in the bottom left of the plate view Fig 9 1 4 click on Sample Click on the Print button to display the report Then click on the top left printing icon to print it The following is an example of a printed sample report 43 User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad Operator manual Manuel utilisateur N S P System Ref Number 90130 10 17 2002 16 05 52 New Sample Pr p eparator Sample ID Sample Plate Status Date of Operation Operation Result Well No Operator Name Sample Plate ID Purification Plate ID Slaughterhouse Name N S P Operation Started at N S P Protocol Completed at N S P Operation Completed at Purification Kit Batch ID Buffer A Batch ID PK Batch ID Buffer B Batch ID Liquid Check Protocol Step Error Record Code Description Sample Report G0200000041 Processed x 2 08 22 2002 10 34 37 NOT COMPLETED D3 1 GILLES P020000004908 P020000004912 1 XXXXXXXXXXXXXXXX 10 05 45 10 34 22 10 36 05 2B0090 52RA1M0030 52PK2C0024 52RB2A0027 Performed All steps preformed 36 Purification Plate short volume Error User manual
25. test is carried out 10 2 2 Weekly maintenance procedure 1 Daily maintenance Refer to 10 1 1 Daily maintenance operation 2 Leaking test 1 Install a tip rack on the table 2 Pour approx 20 ml of distilled water into the waste reservoir and place the reservoir in the buffer A PK position Click on the Leaking test box 4 After the nozzle loads the tips it senses the liquid surface in the reservoir Open the cover when the software is asking for Please open the right system cover to start leaking test procedure 50 User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 6 The nozzles will pipette the water up and down 10 times Then tips are filled with water before staying in the liquid during 60 seconds Close the door when the software is asking If you constate that the liquid level is homogeneous in every tips and if there is no water drip from the nozzle tips for a period of 60 seconds this indicates that there is no problem with leakage 9 Then click yes on the following message 10 Pass the leaking test a liquid level among tips are the same b And no leakage from any tips 11 If there is water drip please change external nozzle O ring reference 35 91 049 See below Replacement of external O ring If the dripping does not stop ins
26. the hand held barcode scanner to scan the plate ID or input by keyboard and press Enter No other entry on this screen is needed Search by both sample plate and Purification plate is possible 9 2 3 Search by reagent kit To search for a processed sample plate using a reagent kit move the cursor to the Batch ID box then use the hand held barcode scanner to scan the batch ID barcode or input by keyboard and press Enter No other entry on this screen is needed 9 2 4 Search by operator name To search for a sample plate that has been processed by a specific operator select the operator number or operator name from the Operator name list To limit the time period of search first enter the time period in the Date boxes then click the Search button 9 2 5 Search by slaughterhouse To search for a sample plate of a specific slaughterhouse select the slaughterhouse code or slaughterhouse name from the Slaughterhouse list To limit the time period of search first enter the time period in the Date boxes then click the Search button 49 User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 9 2 6 Search by time period To search for a sample plate that has been processed within a certain time period enter the time period in the Date boxes When
27. the same time there may be an insufficient volume of electricity Please make sure that the power voltage range is within AC 100 230V 10 when this instrument is operating e Power plug insertion Insert the power plug firmly into the power outlet If not inserted firmly the plug may overheat resulting in fire Carry out the following inspections on a regular basis e Check to make sure that the power plug is inserted into the proper type of power outlet e Check to make sure that the power plug or power cord is not abnormally hot e Check to make sure that the power plug is not cracked or damaged Ground wire connection A CAUTION e To prevent electric shock in the event of a short circuit be sure to connect the ground wire RS232C cable Connect the RS232C cable between the N S P system and the PC Hand barcode scanner PS 2 keyboard connector is required for PC Pull off the keyboard from the PC and attach it to the connector of the barcode scanner then attach the second connector of the barcode scanner to the PC s keyboard connector Fig 3 8 1 Fig 3 8 1 Hand barcode scanner sl Last modification Derni re modification February 2004 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 3 9 Installing the NSP application software The N S P system is operated by exclusive application software installed in a PC Communication between the N S P syste
28. 0 16 2002 10 52 O Service 1 Y N N 10 16 2002 10 54 O Service 1 Y H H 10 16 2002 10 58 3 GIGI 1 Y H H 10 16 2002 12 12 3 GIGI 1 Y N N 10 16 2002 14 18 3 GIGI 1 Y N N 10 16 2002 14 18 3 GIGI 1 Y H H 10 16 2002 14 52 3 GIGI 1 Y N N 10 16 2002 16 20 3 GIGI 1 Y N N 10 16 2002 17 23 3 GIGI O H H H 10 16 2002 17 42 3 GIGI 1 Y H H 10 17 2002 10 43 3 GIGI 0 N N N 10 17 2002 15 53 2 NICO O H H H 10 17 2002 15 56 2 NICO O H H H 10 17 2002 16 01 3 GIGI 1 Y H H 10 17 2002 17 13 2 NICO O N N N 10 18 2002 10 16 2 NICO O H H H 10 18 2002 10 18 3 GIGI O H H H 10 18 2002 10 43 3 GIGI 1 Y N Y 10 18 2002 11 14 3 GIGI O N N N 10 18 2002 11 42 3 GIGI 0 N H H 10 18 2002 11 44 3 GIGI o N N N 1n 1R 2nn9 15 4n 2 GIGI n d VM NI OK 27c Ay 13 53 Start d GE c Sf1Daily Paint QJN S P System Control 50 N 5 P New Sample Pr Ke PIQUE 13 53 Fig 10 4 2 1 Weekly maintenance report The following information can be accessed from the Weekly maintenance report screen Date date the weekly maintenance menu was opened User ID amp User name Id and name of the log in operator Status status of the weekly maintenance at the indicated date and time e indicates that the weekly maintenance menu has been opened and weekly maintenance has been completely performed e 0 indicates that the weekly maintenance menu has not been opened at this date e 1 indicates that the weekly maintenance menu has been open
29. 000088 G0200000096 OK OK OK OK OK OK OK OK OK OK ERROR 43 G0200000017 G0200000025 G0200000033 G0200000041 G0200000049 G0200000057 G0200000065 G0200000073 G0200000081 G0200000089 G0200000097 OK OK OK OK OK OK OK OK OK OK ERROR 43 ns G0200000023 G0200000031 G0200000039 G0200000047 G0200000055 G0200000063 G0200000071 G0200000079 G0200000087 G0200000095 OK OK OK OK G0200000026 G0200000034 G0200000042 G0200000050 G0200000058 G0200000066 G0200000074 G0200000082 G0200000090 G0200000098 OK OK OK OK OK OK OK OK OK ERROR 43 G0200000011 G0200000019 G0200000027 G0200000035 G0200000043 G0200000051 G0200000059 G0200000067 G0200000075 G0200000083 G0200000091 G0200000099 OK OK OK OK OK OK OK OK OK ERROR 43 Error Record Code Description 36 Purification Plate short volume Error 47 Sample not detected 43 Unit Abort Stop BIO RAD 20 47 User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 10 Maintenance and inspection Maintenance 10 1 Daily Maintenance In order to keep the unit clean daily maintenance needs to be carried out once a day after completion of operation Daily maintenance menu can be accessed in either of the following 2 ways 1 Select Daily from the Maintenance in the menu bar Fig 10 1 1
30. 39 90 133 39 90 131 39 90 158 39 90 135 39 91 049 Reference 35 90 200 35 89 158 35 89 046 35 89 198 35 90 134 35 89 190 35 90 136 35 90 155 35 90 156 35 90 138 35 90 137 35 90 140 N S P System Ref Number 90130 Ordering information Description Deepwell microplate Greiner x 50 Sealing film plastic x 50 Sealing film aluminium x 100 NSP Disposable tips x 960 NSP Reservoirs set x 3 NSP Waste container x 20 Nozzle head External O rings 2 x 8 Instrument and accessories Description TeSeE Precess 48 Ribolyser Heating block for 3x20 tubes or 1 deepwell Heating block for 3x20 tubes or 1 deepwell Deepwell rack for heating block 5804R Centrifuge Eppendorf rotor 2 plates NSP Complete system English NSP Complete system French Hand barcode scanner DW 40 Deepwell microplate washer NSP mouse pad These references are included in the NSP Complete system 78 Last modification Derni re modification February 2004
31. 6014 Stacker 2 Limit sensor Timeout 2002 0930 18 47 PO20000005555 Stacker 1 Fail to place TipRack 2002 09 30 19 07 PO2Z0000005555 Stacker 1 Fail to place TipRack 2002 10 01 22 41 PO20000005530 Unit Cover open 2002 1001 22 i POFOO0000SS30 Waste Drawer open 2002 10 01 22 PO20000005530 Unit Abort Stop 2002 10 01 23 16 PO2ZO000005539 Unit Communication Error Fig 11 5 1 1 Error history 2 Use scroll bar to find intended record 3 Click OK to close Error History screen Main menu will be displayed 65 User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 11 5 2 Event log The Event log option gives the description of the last 1000 events occurred on the NSP application software 1 Click Event gt Event log The Event log screen comes up Fig 11 5 2 1 AA BER Event Log Ll Event Name User late MM DD Description Al Configuration Manag 12711 2002 17 26 E Error History OK 1 gilles 12 11 2002 17 2 Click Error History 1 gilles 12 11 2002 17 2 lt Load Configuration Manag 1 gilles 12 11 2002 17 2 lt Click Configuration Manag 1 gilles 12 11 2002 17 2 lt Click Configuration Manag 1 gilles 12 11 2002 16 5 Load Enter Your Password 1 gilles 12 11 2002 16 5 Click Enter Your Password 1 gilles 12 11 2002 16 5
32. 8 22 2002 Operation Result Operation Ready 1 2 3 4 5 6 7 8 9 10 11 12 A e 2002 03 2002 11 2002 19 2002 27 2002 35 2002 43 2002 51 2002 59 2002 67 2002 75 2002 83 B i 2002 04 2002 12 2002 20 2002 28 2002 36 2002 44 2002 52 2002 60 2002 68 2002 76 2002 84 C UN 2002 05 2002 13 2002 21 2002 29 2002 37 2002 45 2002 53 2002 61 2002 69 2002 77 2002 85 D cl 2002 06 2002 14 2002 22 2002 30 2002 38 2002 46 2002 54 2002 62 2002 70 2002 78 2002 86 E o 2002 07 2002 15 2002 23 2002 31 2002 39 2002 47 2002 55 2002 63 2002 71 2002 79 2002 87 F i 2002 08 2002 16 2002 24 2002 32 2002 40 2002 48 2002 56 2002 64 2002 72 2002 80 2002 88 G 20020 2002 09 2002 17 2002 25 2002 33 2002 41 2002 49 2002 57 2002 65 2002 73 2002 81 2002 89 2002 02 H 2002 10 2002 18 2002 26 2002 34 2002 42 2002 50 2002 58 2002 66 2002 74 2002 82 2002 90 19 User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 5 2 Edit Modify a plate layout Edit 1 Select Edit from the Plates menu Fig 5 2 1 BD N 5 P New Sample Pr pjeparator Run Plates Batches Search Maintenance Fig 5 2 1 Edit sample plate Note Only sample plates with Registered status can be edited or modified 2 Select the plate to be modified in the list of plates with registered status Fig 5 2 2 Edit Plate Selection Of x
33. Batches Search Maintenance Mew Delete Fig 6 2 1 Edit reagent batch 2 Select the reagent batch to be edited or modified in the list of reagent batches Fig 6 2 2 5 Batches Edit Reagent Batch Selection ell FS Reagent batch selection Reagent Batches Expiration Dates MM DD YY Select 080012 07 17 2003 Print Cancel Batch ID 080012 Fig 6 2 2 Reagent Batch selection Notes Click on the corresponding column header to sort the Reagent batches by batch number or expiration date The reagent batch already expired is indicated with red characters From this step the list of the Reagent batches can be printed Click on Print see BATCH LIST report on next page 26 User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 Reagent batch list report 10 17 2002 11 24 33 New Sample Pr p eparator BATCH LIST Batch numbers Expiration date 1 2F0017 04 01 2003 2 40171U 08 22 2003 3 2D0023 02 14 2004 4 2F0014 03 06 2004 BIO RAD 1 1 07 User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 The selected reagent batch is displayed Fig 6 2 3 D Plates Edit Reagent Batches Registration R
34. Dates MM DD 080012 07 17 2003 010101 01 01 2003 Delete Print Batch ID 2K0005 Fig 6 3 2 Reagent batch selection 3 Only operator with Manager level is authorized to delete a reagent batch Enter your user name and corresponding password Fig 6 3 3 A Batches Delete Reagent Batch Selection E iol xi Reagent batch selection 080012 07 17 2003 010101 01 01 2003 Print Cancel Enter Your Password User Name OK Password Ga Batch ID 2K0005 Fig 6 3 3 Password entry 4 A Confirmation message is indicated Press YES to confirm and delete the reagent batch 29 User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 7 Operation procedure N S P system operation is carried out using the following procedure Register a New Reagent Batch Batches gt New Register a New Sample Plate New Sample Plate selection Plates gt New New Setup 1 Place the selected sample plate in the units sample plate position 2 Place the empty deepwell plate in the units Run gt Start gt Plate selection purification plate position 3 Fill the reservoirs with the selected batches Reagent Batch selection of Buffer A PK and Buffer B and place them gt Batch selection in th
35. E S EN Reagent batches registration Test ID Code Reagent identification Lot No Expiration date HMD Buffer 4 ER ra femore 061504 a ps2 be eum Print Buffer B 52 Re femoois oeiso4 Buffer C 52 Re fesiziu 071705 Save Purification Kit l0100606445114410080012175 Cancel Fig 6 2 3 Reagent Batch registration Note The following information can be changed from the Batches Edit menu test ID Code lot No expiration date for each reagent buffer A PK buffer B buffer C Only reagent batch that was never been used before for a plate process can be modified 3 When modifications are complete click on Save to register the reagent batch information 6 3 Delete a reagent batch Delete 1 Select Delete from the Batches menu Fig 6 3 1 23 N S P New Sample Pr pjeparator Run Plates Batches Search Maintenance Delete Fig 6 3 1 Delete a reagent batch Note Only reagent batch that was never been used before for a plate process can be deleted 28 User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 2 Select the reagent batch to delete in the list of reagent batch Fig 6 3 2 then click on Delete D Batches Delete Reagent Batch Selection Me x Reagent batch selection Reagent Batches Expiration
36. GILLES O N N 12 17 2002 09 33 1 GILLES 0 N N 12 17 2002 09 32 0 Service 0 N N 12 17 2002 09 23 0 Service 0 N N 12 17 2002 09 18 O Service O N N 12 17 2002 09 17 0 Service D N N 12 17 2002 09 12 0 Service O N N 12 17 2002 09 12 1 GILLES O N N 12 17 2002 09 11 1 GILLES D N N 12 17 2002 09 05 1 GILLES 0 N N 12 17 2002 09 04 1 GILLES 0 N N 12 17 2002 08 52 1 GILLES o N N 12 16 2002 17 46 3 GIGI D N N 12 16 2002 16 03 3 GIGI 0 N N 1 O N N 12 16 2002 14 49 GILLES of d AM erat gt OK D 37C Ay 19 23 Fig 10 4 3 1 Monthly maintenance report The following information can be accessed from the Monthly maintenance report screen Date date the monthly maintenance menu was opened User ID amp User name Id and name of the log in operator Status status of the monthly maintenance at the indicated date and time e 1 indicates that the monthly maintenance menu has been opened and monthly maintenance has been completely performed e 0 indicates that the monthly maintenance menu has not been opened at this date e 1 indicates that the monthly maintenance menu has been opened but the maintenance has not been performed Weekly maintenance status of the weekly maintenance the day the monthly maintenance has been performed Following columns indicate other steps of the monthly maintenance procedure 57 User manual reference number 90362 Last modification Derni re modification February 2004 Version 1
37. K to confirm 3 The Slaughterhouse Property screen comes up Fig 11 2 3 1 Information such as Name of the slaughterhouse address 1 and address 2 city code and city name can be modified x Slaughterhouse Property Slaughterhouse ID i Namen SlaughterhouseA Addressi 1 p Address2 2 7 Codec fi2345 7 City T City a Cancel Fig 11 2 3 1 Slaughterhouse property 4 Change property then click Save 5 Click OK to close the Slaughterhouse screen Main menu will be displayed spi User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 11 3 Password 1 Click on Password in the Configuration menu The Password screen is opened Fig 11 3 1 E Configuration Password Enter Your Password User Mame gilles Enter within S characters Old Passwordio Mew Password OK Confirmation Cancel Fig 11 3 1 Password 2 You can modify the password of the currently log in session 3 Click OK to close the Password screen Main menu will be displayed 11 4 User list 1 Click on User list in the Configuration menu The User list screen is opened Fig 11 4 1 5 Configuration Manager User List User List User List JID UserName User Description User Level Add Manager
38. Load Configuration Manag 1 gilles 12 11 2002 16 5 Click Configuration Manag 1 gilles 12 11 2002 16 5 Load Configuration Manag 1 gilles 12 11 2002 16 5 Click Configuration Manag 1 gilles 12 11 2002 16 5 Load Enter Your Password 1 gilles 12 11 2002 16 55 Click Enter Your Password 1 gilles 12 11 2002 16 5 Load Configuration Manag 1 gilles 12 11 2002 16 55 Click Configuration Manag 1 gilles 12 11 2002 16 5 Load Configuration Manag 1 gilles 12 11 2002 16 55 Click Maintenance Monthly 1 gilles 12 11 2002 15 22 Click Maintenance Monthly 1 gilles 12 11 2002 15 22 Click Maintenance Monthly 1 gilles 12 11 2002 15 22 Load Se Fig 11 5 2 1 Event log 2 Use scroll bar to find intended event 3 Click OK to close Event log screen Main menu will be displayed 11 6 Data location The data location of the Database BSEData and Backup BSEDatabackup files can be changed by following the procedure below 1 Click Configuration gt Data location Note Manager level is requested to modify the location of the database and backup file 2 Enter use name and password Fig 11 2 1 1 The Data location screen will appear Fig 11 6 1 Configuration Data Location Data Location DataBase File NSP System Control Software Data BSEData mdb Browse Backup File bystern Control Sottware Data BSEDataBackup mdb Browse Load Default Settings OK Can
39. N S P New Sample Pr eparator Operator s Manual Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 Before using this product Thank you for choosing the New Sample Pr eparator N S P system The N S P system is an automatic sampling device with 96 well format compatible incubation system that semi automatically carries out the sample purification process for TSE testing Transmissible Spongiform Encephalopathy It provides safe and accurate sample reagent dispensing with a significant reduction of tube handling A windows PC with exclusive application software installed is necessary for operation of the N S P system About this operators manual Please be sure to read this manual thoroughly before using the N S P system o After reading this manual please keep it in a convenient place for quick reference Please observe the products instructions as well as the instructions outlined in the operators manual Please read and follow this manual s safety precautions Instructions in the manual presume that the operator of the N S P system has experience with the general operation method of applications installed ina windows operating system The content of this manual is subject to future change without prior notification Reproduction or copying of this manual without permission is prohibited Although all possible measures have been taken to ensure the accuracy of this manua
40. Operator manual Manuel utilisateur Ref Number 90130 13 User level and access rights rs eat 69 13 1 User level regular users sisi 69 13 2 MARAIS Vel nanas nina die e dt nn EE 69 ON an Oo ne De 69 13 4 User level and access rights 452 5 acido 70 Ta E 71 14 1 Error message screen cece cece ccc cece eee cece eee eee eee eeeeneeesaeeeegeeeeeseeeeeganetsgeeeeeganergs 71 12 Eror Conen A 7 E TEE 71 14 3 Treatment after an error occurrence iii 73 dee IER eege 73 A tee 0 E AE 74 16 1 Configuration of the hand barcode canner UUUU 74 16 1 1 Setting for QWERTY keyboard acccicclennctohccninssiacedesimichenmebbecedeuledbadaisaenedeustannses 74 16 1 2 Setting for AZERTY keyboard ccc cccc ccc e cece cece sence sees sees eaeeeeeeaeeeaeeeeeeaeees 75 16 2 Reagent reservoirs cleaning procedure US 76 16 3 NSP decontamination procedure iii 77 EE e de Le Leite gn TOR eee E ne ame ee came een nee 78 KE AN RE lee 78 16 4 2 Instruments and accessories ccc cece ccc e eee e eee eeceeeeeeaeeeeeeaeeeeeesseesanesaetanenags 78 VII User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 1 Product specifications 1 1 Basic specifications Product name New Sample Pr p eparator System N S P Reference number 90 130 Dimensions 1200 W x 780 H x 430 D mm W
41. Plates Plate selection Sample Plate ID Slaugterhouse ID Select PO20000004484 1 Slaughterhouse 4 16 TA MANUAL Registered Cancel Plate ID D po20000004484 Fig 5 2 2 Plate selection Note Click on the corresponding column header to sort the plates by plate ID slaughterhouse ID date time mode or status 20 User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 3 The selected plate layout is displayed Fig 5 2 3 Plates Edit Plate Registration Plate registration Plate ID pozoo000004927 Operator PIECE Slaughterhouse f1 Slaughterhouse A Well Number Sample ID po oo oo Print Save Cancel Fig 5 2 3 Plates menu Note The following information can be changed from the Edit menu operator name slaughterhouse ID and sample ID 4 When modifications are complete click on Save to register the new plate layout information 5 3 Delete a sample plate Delete 1 Select Delete from the Plates menu Fig 5 3 1 amp NSP New Sample Pripleparator Ruin Plates Batches Search Maintenance Delete Fig 5 3 1 Delete a sample plate Note Only sample plates with Registered status can be deleted ae User manual reference number 90362 Last modification De
42. Ref Number 90130 10 17 2002 16 05 52 P020000004908 P020000004905 Processed x 2 08 22 2002 10 34 37 NOT COMPLETED 18 1 GILLES 1 XXXXXXXXXXXXXXXX 10 05 45 10 34 22 10 36 05 2B0090 52RA1M0030 52PK2C0024 52RB2A0027 Performed 37 0 C 37 1 C 36 9 C OK Last modification Derni re modification February 2004 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 10 17 2002 16 05 52 New Sample Pr p eparator Plate Report Sample Results Sample Plate ID P020000004908 Purification Plate ID P020000004912 Sample Plate Status Processed x 2 Date of Operation 08 22 2002 10 34 37 Operation Result NOT COMPLETED G020000001 2 G0200000020 G0200000028 G0200000036 G0200000044 G0200000052 G0200000060 G0200000068 G0200000076 G0200000084 G0200000092 OK OK OK OK OK OK OK OK OK OK ERROR 43 G0200000013 G0200000021 G0200000029 G020000003 G0200000045 G0200000053 G0200000061 G0200000069 G0200000077 G0200000085 G0200000093 OK OK OK OK OK OK OK OK OK OK ERROR 43 G0200000014 G0200000022 G0200000030 G0200000038 G0200000046 G0200000054 G0200000062 G0200000070 G0200000078 G0200000086 G0200000094 OK ERROR 36 ERROR 47 OK OK OK OK OK OK OK ERROR 43 OK OK OK OK OK OK ERROR 43 G0200000016 G0200000024 G0200000032 G0200000040 G0200000048 G0200000056 G0200000064 G0200000072 G0200000080 G0200
43. Result Waste Tips 160Tips 392Tips OK Fig 7 2 1 Reagent Disposable tips inventory 1 Using the Measured volume screen for reference fill the Buffer A PK reservoir with the appropriate amount of Buffer A PK liquid preparation 4ul of proteinase K for 1ml of buffer A 2 Using the Measured volume screen for reference fill the Buffer B reservoir with the appropriate amount of Buffer B 90 User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 7 3 Tip setup 1 Remove the supply stacker stacker A left side of unit Fig 7 3 1 2 Using the Reagent Disposable tips inventory screen for reference set the tip racks in the stacker Fig 7 3 2 A maximum of 5 tip racks can be loaded in the stacker 3 Install the supply stacker Fig 7 3 3 w A 2 N Y 13 SIA A A CAUTION e The number of tip racks needed for operation will be displayed in the disposable tips box on the Reagent Disposable tip inventory screen Fig 7 2 1 Please be sure to set up a sufficient number of tip racks 1 rack contains 96 tips e Be sure to use new tip racks that contain 96 tips e When there are empty tip racks in the collection stacker be sure to remove them at this time 3199 4 User manual reference number 90362 Last
44. Up to 2 000m AC 100 230V 10 5Cto 50C S y O Upto2 000m gt AC 100 230V EOI pe O Storage temperature range Altitude Supply voltage fluctuations Installation category Pollution degree Power AC 100 240V 10 50 60Hz 100VA Fuse Time lag type AC250V 5A Within 900VA SS User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 1 2 External view and part names Unit Covers Ah SSC Nozzle Head Tip Rack Stackers pe A Wy J Barcode E Scanner ots Waste Tip Drawer Q Abort Button Abort Recover Button Main Switch Power Inlet Earth Connector RS232C Port 20 User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 1 3 Table Table structure Waste Reservoir Tip Eject Assisting Tool Tip Rack Position Sample Position HUE Purification Position Jee LV Gees SN LC XX XX CR A CC CC KC CC CC CT x VC CC CC CC OCOOOOOOCOODOO sees X Git 220000000000 LC CC CC CH KE OOOO OO OO Oe 50 a YL TS LV a KN 0 000 000
45. ample homogenate from each sample plate well is transferred to the purification plate Before each sample transfer NSP system is detecting that the volume of sample to be transferred is sufficient in every wells of the sample plate and each sample is re suspended x2 User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 2 2 2 Pre incubation period The samples that were transferred to the purification plate 2 2 1 on the incubator are heated The 12 minutes pre incubation period is starting since the first sample has been transferred to purification plate Note During pre incubation period NSP system is checking that the volume of liquid in buffer A PK reservoir is sufficient for the quantity of samples to be treated if insufficient reservoir can be replenished on software request 2 2 3 PK treatment 250ul of buffer A PK liquid is added to each purification plate well and mixed thoroughly x 5 The 10 minutes incubation period is starting since buffer A PK has been dispensed and mixed with the first sample in purification plate well Note During PK incubation period NSP system is checking that the volume of liquid in buffer B reservoir is sufficient for the quantity of samples to be treated if insufficient reservoir can be replenished on software request 2 2 4 Precip
46. bruary 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 4 3 Application software start up INFORMATON Before start up of the exclusive N S P system application software be sure to first turn the N S P system s power ON Select N S P system from the start screen s program The password input screen will appear after the title screen 4 3 1 Password entry 4 3 1 1 Initial startup password entry When the software is started up for the first time after installation the manager level password needs to be input and registered Fig 4 3 1 1 1 3 Configuration Manager User List Add User xX Add User Enter New User s infomation User Mame li Description Di User LevelfLi Manager Enter within 5 characters Mew PasswordiN OK Confirmation Cancel Fig 4 3 1 1 1 Add User 1 Input the user name description and confirmation password repeat input of password 2 Confirm with OK button The main screen will appear 4 3 1 2 Following startup When the unit is started up the second time and following the user name and password will normally need to be entered Fig 4 3 1 2 1 ES Password User Name li Po o PasswordiBj Ir Cancel Fig 4 3 1 2 1 Password Entry Input your previously registered user name and password taking care to capital letters or small letters and click OK The Main screen will be
47. cel Fig 11 6 1 Data location 66 User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 11 7 Temperature Consultation of temperature unit and target temperature is available in this option 1 Click Configuration gt Temperature The Temperature Unit screen comes up Fig 11 7 1 E Configuration Temperature Temperature unit Temperature unit c OK Target temperature 370 Offset value 20 Fig 11 7 1 Temperature unit 2 Click OK to close Temperature screen Main menu will be displayed 11 8 Liquid check When liquid check option is activated the NSP system automatically detects the liquid level in every individual well of the purification plate at the end of buffer B dispensing If the volume detected by NSP system is within the acceptable ranges it confirms that sampling has been carried out accurately Liquid Check can be activated un activated in this option 1 Click Configuration gt Liquid Check The Liquid Check option is ON if check mark is placed in the menu Fig 11 8 1 The Liquid Check option is OFF if no check mark is placed in the menu BNP New Sample Pr pjeparator Run Plates Batches Search Maintenance Configuration Help Communication Slaughterhouse Password User L
48. d and ground according to the procedure described in TeSeE package insert chapters 2 6 1 to 2 6 2 2 1 2 2 Sample calibration Remove the grinding tube from the homogenizer resuspend the homogenate by inversion before opening the tubes and take 300yl to 750 ul with the calibration syringe taking care to immerse the needle in the pellet of beads to avoid sampling poorly homogenized tissue fragments Transfer each sample into the sample deepwell plate from position G1 Exclude the first 6 positions A1 to F1 Unused homogenate can be stored at 20 C in the grinding tube In case of subsequent use thaw at room temperature 18 C to 30 C and homogenize after thawing by inversion before taking a new volume of sample Sample can be submitted to a maximum of 3 freezing thawing cycles Note At this stage sample deepwell plate can also be stored sealed with aluminum film ref 90 133 e at room temperature 18 C to 30 C for 8 hours e at 2 C to 8 C in ice or in the refrigerator for 15 hours Samples deepwell plate will need to be adjusted to room temperature 18 C to 30 C for 2 hours minimum before use on NSP Sample purification with TeSeE NSP system Please refer to chapter 6 1 to 6 5 of this manual for operation setup Please refer to chapter 7 1 to 7 4 of this manual to start NSP system operation The following protocol steps will be performed by the NSP system 2 2 1 Sample transfer 250 ul of s
49. e appropriate positions on the table 4 Place new tip racks in the supply stacker Reagent Disposable tips inventory Close the unit s covers gt Reagent Disposable 5 Empty the waste drawer if required Start operation gt Do operation Operation completed Completion of operation is indicated by a warning sound and message See chapter 6 1 to 7 6 for detailed description of each step 30 User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 The following is an explanation of the set up of containers and expendables used for operation Waste Reservoir Tip Eject Assisting Tool Tip Rack Position Sample Position Purification Position fF QOOOQOOOOOOOOO QOQOQOOQOOOOOOO QOOOOOOQOOOOOOO 0000000000 200000000000 EE E ET TEE ok d CC OO OO 50 Ny y N LY a KN O DO 000 000 a YL TS LV a KN O O 0 Buffer A PK Reservoir Tip Shooter Incubator Buffer B Reservoir Heating Block 7 1 Container setup 1 Setthe sample plate in the sample position Set the sample plate in the direction shown in the diagram Fig 7 1 1 The holder is designed so that the sample plate cannot be set in the opposite direction Front 2 Facing the reservoir holder set the Buffer A PK re
50. e barcode label of the Buffer C reagent bottle or input by keyboard and press Enter 6 Make sure that the cursor is in the Purification kit box then use the hand barcode scanner to scan the external label of the Purification Kit or input by keyboard and press Enter Note From this step the Reagent batch can be printed Click on Print see BATCH REPORT on next page 7 When all fields have been entered click the Save button The input Purification kit data will be registered 8 To register other Purification batches repeat the above steps 2 to 7 DA User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 Reagent batch report 10 17 2002 16 05 52 New Sample Pr p eparator BATCH REPORT Batch number 0B0012 Expiration date 07 17 2003 Reagents Batch numbers Expiration dates Buffer A 2M0016 06 15 2004 Proteinase K 8B171U 07 17 2003 Buffer B 2D0023 08 22 2004 Buffer C 2F0014 01 15 2004 BIO RAD 1 1 25 User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 6 2 Edit Modify a reagent batch Edit 1 Select Edit from the Batches menu Fig 6 2 1 D Nep New Sample Pr pjeparator Run Plates
51. e into each purification plate well Do not exceed an interval of 10 minutes between the end of the drying operation and distribution of buffer C Incubate immediately for 5 1 minute at 100 C 5 C Do not seal the plate during incubation Note Heating block must be equipped with a deepwell rack adaptor for heating block ref 90 134 Do not exceed 2 minutes between the buffer C distribution and the beginning of the incubation Remove the purification plate from the incubator Samples can be stored in the purification plate for 5 hours at 2 C to 8 C or frozen for 72 hours at 20 C Frozen samples must be thawed at room temperature 18 C to 30 C Please refer to information on TeSeE detection package insert ref 355 1145 for detailed detection assay protocol User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 3 Setup 3 1 Unpacking the instrument and parts inspection After removing the N S P system from the box first inspect it to make sure that the instrument has not been damaged during shipment Make sure that none of the provided parts are missing If there is any damage to the N S P system or if any parts are missing please contact your Bio Rad representative Provided parts are as follows Items Declaration of conformity Operator s manual NSP quality i
52. ed but the maintenance has not been performed Daily maintenance status of the daily maintenance the day the weekly maintenance has been performed Following columns indicate other steps of the weekly maintenance procedure 56 User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 10 4 3 Monthly maintenance report 1 Select Report from Maintenance in the menu bar 2 Select Monthly report in the selection list indicated The Monthly maintenance report screen will be displayed Fig 10 4 3 1 3 N S P New Sample Pr p eparator Monthly Log P Je x Ey Run Plates Batches Search Maintenance Configuration Help l xj Monthly Log Date MM DD UserID UserName Status WeeklyMaintenance CheckTemperatureOfincubator e g fe J Y PY 12 17 2002 17 45 GILLES 1 12 17 2002 15 59 1 GILLES 0 N N 12 17 2002 15 30 O Service D N N 12 17 2002 15 22 0 Service 0 N N 12 17 2002 15 22 5 MF D N N 12 17 2002 14 32 5 MF D N N 12 17 2002 14 32 5 MF O N N 12 17 2002 14 27 D MF 0 N N 12 17 2002 14 25 1 GILLES D N N 12 17 2002 10 48 1 GILLES 0 N N 12 17 2002 10 48 O Service D N N 12 17 2002 10 46 O Service O N N 12 17 2002 10 45 1 GILLES 0 N N 12 17 2002 10 33 L GILLES D N N 12 17 2002 10 33 O Service O N N 12 17 2002 10 16 0 Service O N N 12 17 2002 10 15 1
53. een instructions Click on the N S P control software button The N S P application software setup screen will open Follow the onscreen instructions 19 User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 4 Turning ON the system 4 1 N S P unit power switch The N S P system s power switch MAIN is located on the front of the unit on the lower right side 1 is ON and 0 is OFF Main Switch 4 2 Abort button 4 2 1 Stopping the N S P system in an emergency To stop the N S P system in the event of an emergency during operation press the Abort button located on the front of the unit on the lower right side Power supply to the unit will be cut allowing the nozzle head and table to be moved by hand Abort Button 4 2 2 Recovery from emergency stoppage Use the following procedure to recover from emergency stoppage of the unit 1 Press the N S P system s Main switch to turn the unit OFF Turn the Abort button clockwise and release it v D Open the cover on the right of the unit and press the Abort Recovery button located on the right side 4 Press the N S P system s Main switch to turn the unit ON Abort Recovery Button SEN User manual reference number 90362 Last modification Derni re modification Fe
54. eight Approx 90 kg Sampling system Tip system 8 nozzles Tip used 300ul disposable tip ref number 90 131 Standard protocol Semi automatic sample purification for Bio Rad TeSeE Assay Deepwell plate Ref number 90132 Reagent reservoirs Ref number 90 158 250ul per nozzle 200ul per well 750ul per well 1 0 lt 1 0 Fixed Mixing method Pipetting Water surface detection Differential pressure method Nozzle Replaceable tip system Tip disposal mechanism Tip disposal box input method Incubator temperature Fixed at 37 C Temp adjustment accuracy 1 0 C Temp adjustment system Silicone rubber heater Built in barcode scanner Optical red LED Hand held scanner LED Good Read Spot beeper Power button Abort button Abort recovery button Liquid surface detection Tip attachment recognition Deepwell plate set up recognition Buttons on unit Sensing mode Reservoir set up recognition Unused tip rack number recognition Used tip rack number recognition Cover open close recognition Windows format PC and N S P system application software Windows 2000 XP RS232C Operation system Operating systems Communication system Environmental requirements Operation environment Operational Environment Operating temperature range Indoor use only 15 C 30 C Maximum relative humidity of 80 for temperatures up to 31 C decreasing linearly to 50 of relative humidity at 40 C 5 C to 50 C
55. el utilisateur Ref Number 90130 14 3 Treatment after an error occurrence Once an error has occurred treatment differs according to the type of error Types of treatment are as follows 2 HALT Press the error message OK button to stop the application To redo the operation again turn the N S P system power off and redo from the beginning 3 CONTINUE Even though error has occurred the operation can be continued or there will be chance to fix the problem during a standby period during operation 4 PAUSE The unit is paused Operation can be resumed once the error is fixed 15 Troubleshooting In case of trouble please refer to the table below to recover the status However if the status does not come to normal stop operation immediately and contact Bio Rad representative No Condition Check item Measure 1 N S P control software can not Isn t the Caps lock of the Cancel Caps lock be activated even though a keyboard effective correct password is entered 2 N S P control software can not y he power cable Connect power cable be activated acd to the outlet Y Are the N S P and the PC Connect RS232C cable connected with RS232C CAD le Y Is power of the N S P Turn the power on a med on fe v s the Abort recover button Turn the Abort recover turn off button on Start of Run menu can not be v Have the BootRun Execute BootRun before l sec Start IP S P doesn t start A A v Are unit covers c
56. eneeeeesee 34 La COCO ports cae cnn ben estenreentesede 34 9 IO EE ee 35 8 1 Sample plate selection iii 35 8 2 e Ee de LEE 36 8 3 Reagent Disposable tips inventory 36 GE DO TA Le E EE 37 GE WEE en AUTO Ee e Le EE 38 8 6 Protocol eau e 39 PPP o 40 DA SC ANGI e ee TE 40 92 Search COMCINON SUID EE 42 9 2 1 Search by sample ENNEN NENNEN eee eeceecsesceeceeceeseeeeseecescseeceecenssesees 42 G22 Carle Dy late 1D eebe Ee 42 923 E A A e e o EER EN E NE EUKIEN 42 9 2 4 Search by operator NAME oocccccccccccccnccncncncnonnnoncnncnnnnnnnrnnnnnnncnnnnnnrornnnnnnnnennss 42 9 2 5 Search by slaughterhouse US 42 AA Ode DY TMG A a ee 43 GE Dala TOPO AAA caen ee dense ta es ra el er eu 43 9 3 1 Sample report printout ccc cece cece cece ee eee eee e ease ese eaeeaseeee eae eateaeeaeeanenas 43 9 3 2 Plate ie Ae dl e E 45 V User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 10 Maintenance and inspection Maintenance 48 10 1 Daily maintenance EE 48 10 1 1 Daily maintenance operations c cece cece cece eee eee e teens eeeee eee esse eneeeegneeeaneeas 49 10 1 2 Daily maintenance procedure Re 49 10 2 Weekly maintenance tee eue creme ae nine 50 10 2 1 Weekly maintenance operations Rs 50 10 2 2 Weekly maintenance procedure Us 50 10 2 3 Replacement of external O
57. er 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 9 Search mode Search Operation results generated during N S P processing are stored in the NSP application software database The NSP application software database is divided in two parts e Transaction file BSEData file contains all results generated during the last 30 days of activity e Back up file BSEDatabackup file contains all results generated over the last 30 days of activity Information in the database can be recalled using the search mode and printed out Backup file is only accessible by search option when the NSP system is not running 9 1 Search procedure 1 Click Search in the menu bar Fig 9 1 1 to open the Search screen Fig 9 1 2 B HSP New Sample Priple Bun Plates Batches Search Sea rch Plate ID Fig 9 1 1 Batch ID Po Operator name po Slaughterhouse M O Datei from Hi i Hn Search Cancel Fig 9 1 2 Search screen 2 In the Search screen set the search conditions refer to 9 2 Search condition setup and then click the Search button The Plate Selection screen will be displayed Fig 9 1 3 amp Search Plate selection Plate selection PO20000004484 1 Slaughterhouse 4 05 06 2002 16 54 MANUAL ProcessedX1 Cancel Pla
58. from the Batches menu Fig 6 1 1 iB Nep New Sample Pr pjeparator Run Plates Batches Search Maintenance Edit Delete Fig 6 1 1 New reagent batch 2 The New reagent batches registration screen is displayed Fig 6 1 2 Make sure that the cursor is in the Buffer A test ID Code box and use the hand barcode scanner to scan the barcode of the buffer A container or input by keyboard and press Enter D Batchs New New Reagent Batches Registration E E x New reagent batches registration Test ID Code Reagent Identification Lot Mo Expiration date MM Buffer A e Buffer B E Buffer C BE Mo EE Save Purification Ku Cancel Fig 6 1 2 New reagent batches 99 User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 3 Make sure that the cursor is in the PK Test ID Code box then use the hand barcode scanner to scan the barcode label of the PK reagent bottle or input by keyboard and press Enter 4 Make sure that the cursor is in the Buffer B Test ID Code box then use the hand barcode scanner to scan the barcode label of the Buffer B reagent bottle or input by keyboard and press Enter 5 Make sure that the cursor is in the Buffer C Test ID Code box then use the hand barcode scanner to scan th
59. ing onm eo gt EH Cancel Fig 8 4 1 Protocol check 1 Select the operator name from the User Name list Confirm that there is a check mark in front of all operation steps Note If instrument covers or waste tip drawer are opened at this stage operations 8 to 8 4 must be repeated 2 Click on Do operation button to start the operation 207 User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 8 5 Screen during operation Run Start Running Protocol Run Plate ID PO20000004475 abort Operator 1 gilles 3 4 7 amp 9 10 11 12 A Ee Sample Transfer no Serer EN W K Ges CR Et Y Pre Incubation DOLO E Bufer APK e Seat ef e Sai ef e zi el 1 01 N HOMO Y Incubation Delay ace i Ee E E Ee E Seen S age Buffer B GE Cy N bebe ut kon CN EENS Ed Liquid Check RES Do d es SE ee du BO MO E Last Heating Time starting 09 15 50 Total time remaining 27min O3sec Column 1 Column 2 Fig 8 5 1 screen during run The Total remaining time indicates the time needed before the end of the operation of the NSP system The following information are indicated e the step under progres
60. intenance is carried out once the message displayed upon application closure will not be activated again until the next day However if the unit is dirtied by spillage of reagent on the table etc we recommend that you carry out daily maintenance again 49 User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 10 2 Weekly maintenance Weekly maintenance is maintenance that is carried out once a week One week after beginning use of the N S P system the first day for weekly maintenance will arrive Weekly maintenance menu can be accessed in either of the following two ways 1 Select Weekly from Maintenance in the menu bar Fig 10 2 1 2 On the day that weekly maintenance is to be carried out if the NSP application software is closed a warning message will appear Operator will be then invited to perform the Weekly Maintenance A INFORMATION Execution of weekly maintenance can be postponed However please be sure to carry out weekly maintenance at the end of the week s operation A Maintenance Weekly Maintenance Weekly Maintenance PF Daily Maintenance Done Leaking test Cancel Fig 10 2 1 Weekly Maintenance screen 10 2 1 Weekly maintenance operations 1 Daily maintenance is carried out Refer to 10 1 Daily maintenance 2 Leaking
61. ist Event Temperature w Liquid Check Fig 11 8 1 Liquid check option 2 Click Configuration gt Liquid Check Note Manager level is requested to change the status of Liquid Check 67 User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 3 Enter user name and password Fig 11 2 1 1 4 Confirm the message in the Protocol Configuration screen then click OK Protocol Configuration x A Liquid check protocol is ON Fig 11 8 2 Liquid check option activated 12 Help 12 1 Operator s manual Help Reference and print out of the Operator s manual in PDF format is available in this option 12 2 About N S P system control software About NSP Version number of the N S P system control software can be referenced in this option 68 User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 13 User level and access rights Some of the software options are protected with a password to ensure high level of safety of all data generated by the NSP system There are three different levels of password protection user level manager lever service level 13 1 User level regular users Unit
62. itation of PrP with buffer B 250ul of buffer B liquid is added to each purification plate well and mixed thoroughly x 5 Buffer B dispensing procedure is the same than buffer A PK dispensing procedure A 5 minutes incubation period is starting since buffer B has been dispensed and mixed with the last sample in purification plate well Note During buffer B incubation period NSP system is checking if liquid check option was selected that the level of liquid in each purification plate well is within acceptable ranges 2 3 Concentration of the PrPres centrifugation Within 15 minutes after the end of operation on TeSeE NSP system centrifuge the purification plate for 10 minutes at 2000 g centrifuge must be set at 4 C Purification plate must be sealed with plastic film during centrifugation ref 90 139 Note Centrifuge must be equipped with deepwell microplate rotor ref 90 136 for 5804R eppendorf centrifuge User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 2 4 Sample clarifying Load the purification plate on DW40 unit Select TSE DW program and select number of strips to be performed Purification plate wells must be dried at the end of the DW40 process by inverting the plate on absorbant paper for 5 minutes Distribute 25 ul of buffer C with a multichanel pipett
63. l s content if you are in doubt about any point or find any errors or omissions please contact your Bio Rad representative Direct all enquiries to BIO RAD Laboratories Life Science Group 3 Boulevard Raymond POINCARE 92430 MARNES LA COQUETTE Tel 33 1 47 95 60 00 Fax 33 1 47 95 61 21 Hot line dpt 33 1 47 95 61 85 Internet http www bio rad com e Windows is a registered trademark of Microsoft Corp USA for the United States and other countries e Pentium is a registered trademark of Intel Corp USA for the United States and other countries User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad Operator manual Manuel utilisateur Safety precautions N S P System Ref Number 90130 Please observe the following cautions to ensure safe and correct operation of this product 1 To ensure safe and correct usage of the N S P system please read these safety precautions prior to use 2 Disregarding these safety precautions may result in injury to the user and or other persons or physical damage to this instrument and or other equipment 3 The following classified precautions indicate the degree of damage or injury that will occur if the particular operation is carried out incorrectly Please read and understand these precautions prior to running the unit User manual reference number 90362 Version 1 17 WARNING
64. losed N S P doesn t start operation x Is waste tip drawer closed a User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 16 Appendices 16 1 Configuration of the hand barcode Hand barcode type MWG100 16 1 1 Setting for QWERTY keyboard If your PC is equipped with keyboard type QWERTY read all codes from here Default configuration Enter configuration Mode PC ALT Mode Barcode font erasement Activate code128 Activate EAN128 Exit and save 74 User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 16 1 2 Setting for AZERTY keyboard If your PC is equipped with keyboard type AZERTY read all codes from here Default configuration Enter configuration PC ALT Mode French keyboard Barcode font erasement Activate code128 Activate EAN128 Exit and save
65. low humidity In places exposed to direct sunlight Directly in front of a stove or other such heating device In places prone to strong vibration In places subject to strong electromagnetic noise or high frequencies 3 3 Heating block Install the heating block in the purification position s incubator The pin on the bottom of the incubator fits in the heating block s hole Align the pin and hole and install in the correct direction Fig 3 3 1 3 4 Supply and collection stacker Install supply and collection stackers Fig 3 4 1 DH dE Aam anao gn D oou A A D 000 Install of the stacker Uninstall of the stacker Fig 3 4 1 10 User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad Operator manual Manuel utilisateur 3 5 3 6 3 7 3 8 User manual reference number 90362 Version N S P System Ref Number 90130 Power supply requirements Insert the power plug directing into a single phase AC100V 230V 50Hz or 60Hz power outlet that has a current capacity of over 5A and a voltage fluctuation range of 10 For PC power specifications please refer to your PC s operators manual A CAUTION e Confirming electricity volume Because most power outlets of laboratories and other such places are used to supply power to various electric components at
66. lt 5 If the measured volume is in the 28 ml to 32 ml range liquid sensing is normal 6 If the measured volume is outside the 28 000 ul to 32 000 ul range inspection by a service engineer will be necessary In this case please contact Bio Rad 5 When all maintenance functions have finished confirm that all boxes have a check mark and then click Done 6 Completion of monthly maintenance will be recorded 54 User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 10 4 Maintenance Report 10 4 1 Daily maintenance report 1 Select Report from Maintenance in the menu bar 2 Select Daily report in the selection list indicated The Daily maintenance report screen will be displayed Fig 10 4 1 1 amp N S P New Sample Pr p eparator Daily Log e x 4 Run Plates Batches Search Maintenance Configuration Help a x Daily Log 10 09 2002 16 48 2 NICO 1 Y Y Y Y Y Y Y Y Y 10 10 2002 10 13 1 GILLES 1 N N N N N N N N N 10 10 2002 10 37 1 GILLES 1 Y Y Y Y Y Y Y Y Y 10 11 2002 15 17 2 NICO 1 N N N N N N N N N 10 11 2002 15 19 2 NICO o N N N N N N N N N 10 11 2002 15 20 2 NICO o N N N N N N N N N 10 11 2002 15 28 2 NICO o N N N N N N N N N 10 11 2002 15 32 2 NICO o N N N N N N N N N 10 11 2002 15 45 2 NICO o N N N N N N N N
67. lug unplug by holding the power plug not by pulling on the power cord Pulling on the cord may lead to breakage of the cord II User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 Contents Wg E SEENEN Ate 1 Vel BASIC SOCCINCANOMS ES nee ue ea ne ns dt eee ee Nu 1 1 2 External view and part names iii 2 lo aple AS A a Ee 3 le CONTADOS seran osos 3 2 nr WEE 6 2 1 Sample e e e EE 6 2 2 Sample purification with NSP system US 6 E WE e Een E 6 2 2 2 Pre incubation period ccc ccc ce eee ee cece eee eee eee eee eeeeeeeeaaeeseaeaeeeeeaneeeesguneenggas 7 223 A o o o e ro oo EAE ERRA OESTE D 2 2 4 Precipitation of PrPres with buffer H 7 2 OCENIO EE EE EE 7 2A Eu ee E Ou e EE 8 GE a E o ne de A 9 3 1 Unpacking the instrument and parts inspection US 9 3 2 SCUD SAVIO TT EE 9 oo re DIC E 10 3 4 Supply and collection Ee E 10 3 5 Power s pply Fequiremenls EEN 11 36 Ground Wife COMMECUON EE 11 Bel A e PP EEEE E A 11 3 8 Hand barcode scanner 11 3 9 Installing the NSP application software 12 A Re E 12 3 9 2 Installation procedure c cece cece ec ee sce e seen esses aceeseeesaseeesenseeesaeeeasenganeasenes 12 4 Turning ON the system EE 13 GC NGP UDI DONS SWIC EE 13 de o UE 13 4 2 1 Stopping the N S P system in an emergency oocccoccocconccnconcncnnannn
68. m and PC is via the RS232C cable 3 9 1 PC environment The PC environment needed for installation and control of the N S P system application software Is as follows Operation system Windows 2000 CPU Pentium III or above or a PC AT converter that includes a completely compatible equal or higher CPU Screen size 1024 x 768 pixel selectable Memory capacity Above 256MB Hard disc capacity Above 5GB RS232C port D sub 9 pin Power supply When using a desk top PC connect the PC to a power supply that is not subject to power outages Others The PC should be equipped with a P S2 terminal FD drive CD Rom drive and USB port 3 9 2 Installation procedure A CAUTION e The exclusive N S P system application software is contained within 1 CD ROM and 1 floppy disc Before installation we recommend that you make back up of the original discs and install the software using the back up discs Close all other applications currently running before installing the N S P system application software In the event that the software cannot be installed using the following procedure please contact your Bio Rad representative Start up the Windows application Insert the N S P system application software CD ROM into the PC s CD ROM drive The installation screen will be automatically displayed Click on the Adobe button the click on the Adobe icon The Adobe setup screen will open Follow onscr
69. nce at the indicated date and time e 1 indicates that the daily maintenance menu has been opened and daily maintenance has been completely performed e OT indicates that the daily maintenance menu has not been opened at this date e 1 indicates that the daily maintenance menu has been opened but the maintenance has not been performed Following columns indicate every step of the daily maintenance procedure 55 User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 10 4 2 Weekly maintenance repot 1 Select Report from Maintenance in the menu bar 2 Select Weekly report in the selection list indicated The Weekly maintenance report screen will be displayed Fig 10 4 2 1 amp N S P New Sample Pr p eparator Weekly Log K i f xj Z7 Run Plates Batches Search Maintenance Configuration Help lej xj Weekly Log Date MM DD DailyMaintenance LeakingTest CheckHorizontalLevel gt 10 09 2002 16 55 2 d 10 16 2002 09 51 2 NICO 0 N N N 10 16 2002 09 52 2 NICO O H H H 10 16 2002 09 56 2 NICO O N N N 10 16 2002 09 57 2 NICO O H H H 10 16 2002 10 28 2 NICO 0 N N N 10 16 2002 10 29 3 GIGI O N N N 10 16 2002 10 30 3 GIGI 1 AN N N 10 16 2002 10 40 3 GIGI 1 Y H H 10 16 2002 10 41 O Service 1 Y H H 10 16 2002 10 48 U Service 1 Y N N 1
70. nnncannnnnnnnnannnnao 13 4 2 2 Recovery from emergency stoppage ss 13 AS ADDIICAlION soitware start UP EE 14 43 1 PassWord MIY EE 14 4 3 1 1 Initial startup password entry ccc eee eee eect estes esate eaeeeaeeeaeenaeenes 14 4 3 1 2 Following NS ADS es che aan ca han co eines ce cue 14 432 Main Screen SIar sionista dia tea 15 4 4 Closing application software cococccocccoccnoccconncocnnonnnnnconnnnnnconnnnnncnnnannnannnannnnnnnns 16 IV User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 Se O o ne PESO EOS 17 5 1 Registration of a new sample plate New nnnnnnnnnnnnnnnnnnnnnnununnrrrenrrrrrrrrrrrrrrrrrrrene 17 5 1 1 Manual entry mode cccc ccc ccc cece cece cence eee eee e sees sees eeeeeaeeeaateeaeeeaeeaanenanens 18 5 1 2 Automatic increment entry mode 18 5 2 EGIUMOGITY plate layout Edil enue deeg docto e 20 5 3 Delete a sample plate Delete US 21 6 Batches M NU EE 23 6 1 Registration of a new reagent batch New 23 6 2 Edit Modify a new reagent batch Edit 26 6 3 Delete a reagent batch Delete ccc ccc cece cece cece cece eeeaeeeaeeeaeesaneeaeeeanerenenanes 28 7 Operation procedure iii neeeeeneeeeeeneeneeemeemeeeeeemeenesee 30 TA el SSD a ee en ee ne die 31 Pec PRO Be 32 e d Me SOUND EE 33 7 4 Waste tip drawer sisi eseeeeee
71. nspection report Stacker A Supply Stacker B collection Heating block for incubator Power cable French type RS 232C cable D sub 9 pin TeSeE NSP application software CD x 1 Individual setting value for TeSeE NSP FD x 1 3 2 Setup environment To ensure safe and proper operation of the unit please be sure to observe the following when installing the instrument 1 Install on a stable flat surface The N S P system weighs approx 90 kg Install on a flat surface that can support the unit s weight 2 Install in a well ventilated area that is not dusty The N S P system has ventilation openings for heat radiation on the back panel Install the unit in a well ventilated place making sure that the rear side of the unit is 10 cm or more from any wall 3 Operate in an environment that has a suitable temperature and humidity Operating temperature range 15 C to 380 C Maximum relative humidity of 80 for temperatures up to 31 C decreasing linearly to 50 of relative humidity at 40 C 4 Avoid installing in the following places e Places that are not sturdy or not level 9 User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 Places that have poor ventilation and are very dusty Places with high temperature excessive humidity low temperatures or
72. ntaminating solution e Rinse with ethanol e Dry on a paper towel 4 Frequency of replacement Although the waste and buffer reservoirs and associated covers are correctly maintained see cleaning procedure above their use must be limited to 4 weeks maximum 76 User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 16 3 NSP decontamination procedure Biosafety measures must be in agreement with recommendations of regular authorities of the country 1 2 User manual Version 1 17 All the materials directly in contact with the samples and the wasted liquids must be considered as contaminated Contaminated surfaces of the NSP system tip rack position buffer A PK position buffer B position waste position must be cleaned with 6 chlorometric CHL sodium hypochlorite solution bleach Surfaces must be rinsed with a paper towel soaked in distilled water then dried with ethanol The material use for cleaning must be discarded in a special container for contaminated wastes 77e reference number 90362 Last modification Derni re modification February 2004 Bio Rad Operator manual Manuel utilisateur 16 4 alternative alternative User manual reference number 90362 Version 1 17 16 4 2 16 4 1 Disposables Reference 39 90 132 39 90 139
73. odification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 11 2 Slaughterhouse 1 Click on Slaughterhouse in the Configuration menu The Slaughterhouse screen is opened Fig 11 2 1 and the slaughterhouses already defined are listed Configuration Slaughterhouse Slaughterhouse Slaughterhouse List 2 Slaughterhouse B Delete 3 Slaughterhouse Property OK Cancel Fig 11 2 1 Slaughterhouses list 11 2 1 Addition of a new slaughterhouse 1 From the Slaughterhouse screen Fig 11 2 1 click Add button Note Manager level is requested to define a new Slaughterhouse 2 Enter your User name and password Fig 11 2 1 1 then click OK to confirm Enter Your Password User Name OK F d asswor SoReal Fig 11 2 1 1 Password entry 60 User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 3 The Add Slaughterhouse screen comes up Fig 11 2 1 2 Information such as Name of the slaughterhouse address 1 and address 2 city code and city name can be entered x Add Slaughterhouse Slaughterhouse IDI 004 Cave Home Ir Address Te Address2 2 Te Code City T FO Cancel Fig 11 2 1 2 Add slaughterhouse 4
74. pection by a service engineer will be necessary In this case please contact Bio Rad 4 As each maintenance function is carried out add a check mark in front of the maintenance item displayed on the weekly maintenance screen Fig 10 2 1 by clicking the box in front of that item 5 When all maintenance functions have finished confirm that all boxes have a check mark and then click Done 6 Completion of weekly maintenance will be recorded Note When weekly maintenance is carried out once the message displayed upon application closure will not be activated again until the next week 10 2 3 Replacement of external O ring Note The following procedure must be applied only if leaking was detected during weekly maintenance procedure 1 Open the right side of the unit cover 2 Remove external O rings with forceps Fig 10 2 3 1 Fig 10 2 3 1 Removing O ring 51 User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 3 Place smaller O ring in the bay near to the top of the nozzle Fig 10 2 3 2 10 2 3 3 Please do not place bigger O ring first since it can be stuck in the bay for smaller O ring eee oils Fig 10 2 3 2 Placing smaller O ring Fig 10 2 3 3 Small O ring in the bay m UI 4 Place bigger O ring in the pay Fig 10 2 3 4 10 2 3 5
75. proceed to step 8 5 1 2 Automatic increment entry mode 7 To continue editing samples with the automatic increment entry mode click on the intended well and while holding simultaneously the mouse s left button down and control Ctrl key drag the mouse up to the last sample position The highlighted area will automatically be incremented and registered one by one starting from the first sample ID input Fig 5 1 2 1 x New plate registration Plate 1D PO20000004871 O Operator 2 giles y A o e 3 o ry Yor D SO Well Number Be 1906000808 0000 Sample ID cesse 0 1000000000000 E 200000 O re Print 000000000000 E 6 00006000000 1 1980000 0000 cmai S Fig 5 1 2 1 Sample entry Note From this step the plate layout can be printed Click on Print then confirm that the plate layout will be saved before to be displayed see next page 8 When sample registration is completed click on the Save button The input sample IDs will be registered SSES User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 Plate layout report 10 17 2002 16 05 52 New Sample Pr p eparator Plate Layout Report Sample Result Plate ID P020000004908 Sample Plate Status Registered Date of Registration 0
76. removed from the table al Buffer B reservoir is washed Waste reservoir is removed from the table N Waste reservoir is washed If there is sample waste liquid in the reservoir first dispose of it NNN Nm TN em Nem ml OS O0 O G ee a eg A A A A seg A Sample plate is removed from the table O Purification plate is removed from the table k Waste tip container is removed from the tip waste drawer and disposed of 11 Tip racks are removed from the stackers supply stacker and collection stacker can be removed from the machine 12 With a swab soaked in distilled water clean correctly every sensor on the worktable tip rack position buffer A PK position buffer B position waste position Then clean movable table with a paper towel soaked in distilled water then dry with ethanol 13 Liquid drop prevention tray is cleaned using paper towel soaked in distilled water then ethanol 14 Tip eject assisting tool is cleaned with paper towel soaked in distilled water then ethanol 10 1 2 Daily maintenance procedure 1 As each maintenance function is carried out add a check mark in front of the maintenance item displayed on the Daily maintenance screen by clicking the box in front of that item Fig 10 1 1 2 When all maintenance functions have finished confirm that all boxes have a check mark and then click Done 3 Completion of Daily maintenance will be recorded Note When daily ma
77. rni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 2 Select the plate to delete in the list of plates with registered status Fig 5 3 2 then click on Delete Plates Delete Plate Selection ell a Plate selection Sample Plate ID Slaugterhouse ID Date Time Mode Status 13 30 MANUAL Registered Delete Cancel Plate ID I P200206070001 Fig 5 3 2 Plate selection 3 Only operator with Manager level is authorized to delete a plate Enter your user name and corresponding password Fig 5 3 3 Plates Delete Plate Selection ee BER Plate selection 06 07 2002 13 30 MANUAL Registered Cancel Enter Your Password _ Cancel User Name OK Password PR Plate DU P200206070001 Fig 5 3 3 Password entry 4 A Confirmation message is indicated Press YES to confirm and delete the plate EGT User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 6 Batches menu Select Batches from the Main menu to open the Batches menu BNP New Sample Pr p eparator Run Plates Batches Search Maintenance New Edit Delete Fig 6 1 Batches menu 6 1 Registration of a new reagent batch New 1 Select New
78. run Processed x2 for a second run NO Operator s name GN Sample plate ID Batch ID for the purification kit odo P ID for each reagent buffer A Proteinase K buffer B buffer C O Slaughterhouse ID 7 Data for each well The following data for operations up to the second operation that have been carried out for specified sample plate can be viewed on the lower part of the plate view screen 1 Sample position well number and sample ID NO Protocol step number and designation Time the corresponding protocol step was started Minimum and maximum temperature during the step 5 Comment result SES User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 9 2 Search condition setup Search Sample ID FO Plate ID PO Batch ID FO Operator name o Slaughterhouse M Ci Y Date D from Hi Hn Hn Search SS Cancel Fig 9 2 1 Search condition setup 9 2 1 Search by sample To search for a specific sample move the cursor to the Sample ID box then use the hand held barcode scanner to scan the sample ID or input by keyboard and press Enter No other entry on this screen is needed 9 2 2 Search by plate ID To search for specific sample plate data move the cursor to the Plate ID box then use
79. s is circled in red right part of the screen e the step s already preformed are highlighted in purple right part of the screen e the samples under progress are circled in red in plate layout e the sample successfully treated are highlighted in purple in plate layout e if an error has been detected during a step the corresponding sample positions remain uncolored in plate layout 38 User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 8 6 Operation complete When the delay period ends a warning sound will be heard and a message will be displayed to indicate that operation has finished Run Start Running Protocol Run Plate ID PO2O0000004464 Operator H Ooilles Y Sample Transfer Y Pre Incubation Buffer aer Y Incubation Delay Protocol Complete a Operation is completed H i dE ei OO You can remove the Purification Plate now Wel ES Liquid Level 24 38mm WellS3 Liquid Level 24 26mm Well H3 Liquid Level 24 22mm lt Last Heating gt lt Protocol Complete gt Fig 8 6 1 NSP operation complete Open the right instrument cover to take the purification plate and perform the next step of the TeSeE assay Opening the right instrument cover will automatically end the NSP protocol 39 User manual reference numb
80. se the hand barcode scanner to scan the barcode of the new deepwell plate or input by keyboard and press Enter Plates New New Plate Registration New plate registration Plate ID L Well Number Sample ID Print Save Cancel BOO E00 0000 A Jo OO OOOO CCE yr oro HANSON OOOO Fig 5 1 2 New Plate registration 3 Click on the right edge of the Operator box and select the operator name in the list 4 Click on the right edge of the Slaughterhouse box and select one of the slaughterhouse ID in the list Eye User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 5 The G1 well in the deepwell plate layout is circled in red and the position G1 is indicated in the Sample ID box Make sure that the cursor is in the Sample ID box then use the hand barcode scanner to scan the barcode of the first sample or input by keyboard and press Enter The sample will be registered in the well and the next well position H1 will be circled Note Sample Ids are 11 characters maximum Both alphabetic and numeric characters can be used to identify the samples 5 1 1 Manual entry mode 6 To continue editing samples manually repeat the above 5 step for the number of samples that you wish to process when sample editing is completed
81. servoir on the left side and the Buffer B reservoir on the right side Set with the A PK or B indication facing to the front 3 Facing the reservoir holder set the waste reservoir on the right side set in the same direction than the two others 4 Install an empty deepwell plate in the Heating block on top of the purification plate position Set securely in the correct position The heating block is designed so that the purification plate cannot be set in the opposite direction et oe User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 7 2 Filling containers The volume of reagent needed will differ depending on the number of samples The volume needed for the operation that will be carried out will be displayed on the Reagent Disposable tips inventory screen Fig 7 2 1 amp Run Start Reagent Disposable tips inventory loj x Reagent Disposable tips inventory Reagents Description Required Buffer 4 PK 12000uL Buffer B 12000uL Disposable Tips Description Required Detected Result Disposable Tips Left 160Tips 2Racks 384Tips 4Racks OK Empty Racks 4rea Right 2Racks SRacks OK Cancel Plates Description Required Detected Result Sample Plate ID PO20000006040 PO20000006040 OK Purification Plate ID PO20000006001 OK Waste Bag Description Required Detected
82. te ID D Po20000004484 Fig 9 1 3 Plate selection screen Note Plates can be stored by column criteria if you click on the corresponding column header 40 User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manuel utilisateur Ref Number 90130 3 In the Plate Selection screen select one plate from the plate list then press Select button The Plate View screen will be displayed Fig 9 1 4 Search Plate view e xl Plate DEE Plate Status Processed x1 DI Date 05 01 2002 4 2 3 4 5 6 7 8 9 10 11 12 Operator Name Naoki Takata H iD ES VV NV WV V DO Plate ID Poooo0000001 yO CH Batch ID 50xx1D0018050102 Buffer A ID 50R41D0018050102 Buffer PK ID 50PK1D0018050102 Buffer B ID 50RB1D0018050102 Buffer C ID fS0RC1D0018050102 Slaughter House ID 001 Ueno Kaitai Sample Well Number G1 Sample ID 001 Ueno Kata Protocol Time Temp reng d Result acseveceevcvcessveversuserecesessceuessuecevsusesecenescvecescuscssccscseveseveuesesccncessosesers Complete Sg Sp rin C Sample Fig 9 1 4 Plate view The following data for operations up to the second operation that have been carried out for specified sample plate can be viewed on the upper part of the plate view screen k Plate statuses Processed x1 for a first
83. te over volume Error Too much liquid volume in Purification Plate Liquid level sensor Error Liquid level sensor fault when Reservoir is used 39 Waste Drawer open HALT Waste drawer opened during protocol operation 40 TipRack Lock Front sensor Timeout HALT No response from TipRack lock system front sensor 41 TipRack Lock Rear sensor Timeout HALT No response from TipRack lock system rear sensor Buffer B Reservoir set Error Reservoir is not mounted Unit Abort Stop HALT Abortion of operation Database update Error HALT Update of the database interrupted 45 Tip catch failed gt Retry same row Continue If fail to catch tip for the first time in the row try same row again proceed to the next row 47 Sample not detected Sample in the sample plate does not exist or volume of sample is lower than requirement Need to add Buffer A PK for operation to complete Operation can be continued after addition of Buffer A PK complete Operation can be continued after addition of Buffer B 50 TipRack Addition Pause Need to add TipRack for operation to complete Operation can be continued after addition of TipRack 51 Tip catch failed HALT After two cycle of Error 45 and 46 if Tip catch failed occur again on the same TipRack the operation will be aborted Be GR User manual reference number 90362 Last modification Derni re modification February 2004 Version 1 17 Bio Rad N S P System Operator manual Manu
84. these areas may result in OQ electric shock Contact your contact your Bio Rad representative when inspecting or repairing the internal parts Do not bend pull twist or stand on the power cord This may cause breakdown or fire Do not remove the units cover or alter or modify the internal parts This may cause damage or result in electric shock etc In the event of breakdown stop usage immediately and request that your Bio Rad representative repair the unit Do not use the unit while broken or have it repaired by anyone Do not place your hands or any part of your body near the unit while it is operating If you need to touch the unit etc first stop it before doing so Also be sure to use caution when restarting the unit gt CAUTION Category Do not use near fire or in places subject to high temperature or high humidity Doing so may cause an accident Make sure that the rear of the unit is 10 cm or more away from any wall If placed near a wall the internal temperature will rise and the unit may emit smoke Also do not place anything on top of the unit This may cause breakdown ECS Do not use the unit outside the Bio Rad s standard specifications This may damage to the unit or cause an accident Do not use the unit outside the indicated power voltage The may result in fire or electric shock A When using be sure to connect the power cable to a power outlet that is grounded When disconnecting the power p

TeSeE NSP Operator`s Manual Ver. 1.16 - Bio-Rad

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