JS-5.3 Rotor
Contents
1. max av m min 0 1000 2 000 3 000 Speed rpm 4 000 5000 5300 19 JS 5 3 Rotor 20 CARE AND MAINTENANCE MAINTENANCE Do not use sharp tools on the rotor that could cause scratches in the rotor surface Corrosion begins in scratches and may open fissures in the rotor with continued use Periodically at least monthly inspect the rotor yoke and buckets especially inside cavities for rough spots or pitting cracks white powder deposits frequently aluminum oxide or heavy discol oration If any of these signs are evident do not run the rotor Contact your Beckman Coulter representative for information about the Field Rotor Inspection Program and the rotor repair center Before using the rotor tie down knob 368410 check it for damage such as distortion splitting or stripped threads Replace it if itis damaged Before each use inspect the covers to make sure they are in good condition Do not use a cover that is cracked or damaged Inspect each O ring to make sure that is smooth and free of nicks tears and abrasions Make sure that it has not stretched beyond its original shape and size Inspect the top edge of the rotor bucket to make sure that is clean and smooth nspect the bucket cover air vent filters before each run to make sure that they are clean If a filter is discolored replace it by gently pushing it out from underne2. Insert a new air vent filter into each cover as described in MAINTENANCE above Air vent filter Figure 8 Aeroseal Cover 22 DECONTAMINATION JS 5 3 Rotor If aluminum rotor components become contaminated with radioactive material decontaminate them using a solution that will not damage the anodized surfaces Beckman Coulter has tested a number of solu tions and found two that do not harm anodized aluminum RadCon Surface Spray or IsoClean Solution for soaking and Radiacwash While Beckman Coulter has tested these materials and found that they do not damage components no guarantee of decontamination is expressed or implied Follow appropriate decontamination proce dures as directed by your laboratory safety officer If the rotor or other components are contaminated with toxic or pathogenic materials follow appropriate decontamination procedures as directed by your laboratory safety officer Refer to the chemical resistance chart in Appendix A of Rotors and Tubes to ensure that procedures will not damage the components STERILIZATION AND DISINFECTION Cooma up 121 C The rotor can be autoclaved at 121 C for up to an hour Plastic parts can be autoclaved at 121 C for up to 30 minutes Place the rotor yoke buckets and or microplate carriers in the autoclave upside down The bucket cover with air vent filter removed can be autoclaved at 121 C for up to 30 minutes Before autoclaving remove the
3. Tube Bottle Adapters Symmetrical and Balanced Loading To ensure optimal performance and stability the rotor must be loaded symmetrically see Figure 2 Two factors affect symmetric loading The buckets must be loaded symmetrically with respect to their pivotal axes The rotor should be loaded symmetrically with respect to its center of rotation For best results load opposing buckets with similar adapter or carrier types and the same type of labware containing the same amounts of fluid of equal density within 10 grams To prevent imbalance and reduce centrifuge drive wear the weight of adjacent buckets should be within 220 grams The maximum load for buckets is 1040 grams each During a run buckets swing 90 degrees from their at rest position The pivotal axis of a bucket can be imagined as a line extending across the bucket from one pivot pin to the other If a bucket is loaded so that its weight is unequally distributed on either side of its pivotal axis it will not hang vertically at rest and more importantly may not swing to a horizontal position during a run As a result extra stress will be placed on the bucket adapter carrier tubes and or multiwell plates during the run increasing the possibility of breakage or rotor imbalance In multiwell plates samples should be loaded into the wells symmet rically with respect to the pivotal axis of the carrier the pivotal axis runs parallel to the crossbar and opp
4. filter from each cover as described in MAINTENANCE above After autoclaving insert a new air vent filter into each cover Ethanol 7096 or hydrogen peroxide 6 may be used on all rotor components including those made of plastic Bleach sodium hypochlorite may be used but may cause discoloration of anodized surfaces Use the minimum immersion time for each solution per laboratory standards Cold sterilization methods such as ethanol 70 hydrogen peroxide 10 Wescodyne or Cidex may be used on bucket covers Consult Appendix A in Rotors and Tubes before using any other sterilization methods 3 In U S A contact Nuclear Associates New York in Eastern Europe and Commonwealth States contact Victoreen GmbH Munich in South Pacific contact Gammasonics Pty Ltd Australia in Japan contact Toyo Medic Co Ltd Tokyo 4 In U S A contact Biodex Medical Systems Shirley NY internationally contact the U S office to find the dealer nearest you 5 Flammability hazard Do not use in or near operating centrifuges 6 Registered trademark of West Chemical Products Inc 7 Registered trademark of Arbrook Inc Cidex is a gluteraldehyde 1 5 pentanedial product 23 JS 5 3 Rotor 24 STORAGE RETURNING A ROTOR While Beckman Coulter has tested these methods and found that they do not damage the rotor or components no guarantee of sterility or
5. Coulter s warranty obligations with respect to any rotor and or centrifuge in which such a bucket is used DISCLAIMER IT IS EXPRESSLY AGREED THAT THE ABOVE WAR RANTY SHALL BE IN LIEU OF ALL WARRANTIES OF FITNESS AND OF THE WARRANTY OF MERCHANT ABILITY AND THAT BECKMAN COULTER INC SHALL HAVE NO LIABILITY FOR SPECIAL OR CON SEQUENTIAL DAMAGES OF ANY KIND WHATSO EVER ARISING OUT OF THE MANUFACTURE USE SALE HANDLING REPAIR MAINTENANCE OR REPLACEMENT OF THE PRODUCT Beckman Coulter Worldwide Biomedical Research Division Offices AUSTRALIA Beckman Coulter Australia Pty Ltd Unit D 24 College St Gladesville NSW 2111 Australia Telephone 61 2 9844 6000 or toll free 1 800 060 880 Fax 61 2 9844 6096 email lifescienceaustralia 9 beckman com CANADA Beckman Coulter Canada Inc 6755 Mississauga Road Suite 600 Mississauga Ontario Canada L5N 7Y2 Telephone 905 819 1234 Fax 905 819 1485 CHINA Beckman Coulter Inc Beijing Representative Office Unit 2005A 2006 2009 East Ocean Center Jian Guomenwai Avenue Beijing 100004 China Telephone 86 10 6515 6028 Fax 86 10 6515 6025 6515 6026 EASTERN EUROPE MIDDLE EAST NORTH AFRICA Beckman Coulter International S A 22 Rue Juste Olivier Case Postale 301 303 CH 1260 Nyon Switzerland Telephone 41 22 994 07 07 Fax 41 22 994 07 00 FRANCE Beckman Coulter France S A Paris Nord II 33 66 rue des Vanesses B P 50359 Villepin
6. Primegro Park Tonetti Street 1685 Halfway House Johannesburg Republic of South Africa Telephone 27 11 805 2014 5 Fax 27 11 805 4120 e mail beckman intekom co za Internet www beckmancoulter com Telex 678413 2005 Beckman Coulter Inc SPAIN Beckman Coulter Espa a S A C Caleruega 81 28033 Madrid Spain Telephone 91 3836080 Fax 91 3836096 email bioresearch espana 9 beckman com SWEDEN Beckman Coulter AB Archimedesvaegen 7 Box 111 56 SE 168 11 Bromma Sweden Telephone 0 8 564 85 900 Telefax 0 8 564 85 901 SWITZERLAND Beckman Coulter International S A 22 Rue Juste Olivier Case Postale 301 303 CH 1260 Nyon Switzerland Telephone 0800 850 810 Fax 0848 850 810 TAIWAN Beckman Coulter Taiwan Inc Taiwan Branch 8th Floor 216 Tun Hwa South Road Section 2 Taipei 106 Taiwan Republic of China Telephone 886 2 2378 3456 Fax 886 2 2377 0408 TURKEY Beckman Coulter Ltd E 5 Yanyol Faith Cad 81410 Soganlik Kartal Istanbul Turkey Telephone 90 216 309 1900 Fax 90 216 309 0090 UNITED KINGDOM Beckman Coulter United Kingdom Ltd Oakley Court Kingsmead Business Park London Road High Wycombe Buckinghamshire HP11 1JU England U K Telephone 01494 441181 Fax 01494 447558 e mail beckman uk beckman com Authorized dealers in other countries 4300 N Harbor Boulevard Box 3100 Fullerton California 92834 3100 e Fax 1 800 643 4366 Cry Q
7. according to the following equation 1040 grams reduced maximum speed 5300 rpm heaviest load in grams Do not select rotational speeds in excess of 5300 rpm I gt NOTE Polystyrene plates are subject to cracking at high speeds Test polystyrene plates using water in place of sample to determine required speed deration 15 JS 5 3 Rotor Table 1 Microplates Used in the JS 5 3 Rotor High throughput processing kits are available commercially observe manufacturer s recommendations for speed and temperature limitations Part Accessory Part Description Volume Number Description Number multiwell polystyrene plate 96 well 300 uL well 609844 cap strip nonsterile 267002 pkg 12 nonsterile pkg 100 cap strip sterile 267005 pkg 12 aluminum foil lid 538619 pkg 100 deep well polystyrene plate 96 well 1 mL well 267001 cap strip nonsterile 267002 pkg 12 nonsterile do not stack these plates pkg 24 cap strip sterile 267005 pkg 12 aluminum foil lid 538619 pkg 100 deep well polystyrene plate 96 well 1 mL well 267004 cap strip nonsterile 267002 pkg 12 sterile do not stack these plates pkg 24 cap strip sterile 267005 pkg 12 aluminum foil lid 538619 pkg 100 deep well polypropylene plate 96 well 1 mL well 267006 cap strip nonsterile 267002 pkg 12 nonsterile pkg 24 l cap strip st
8. at its election by replacement any defects of material or workmanship which develop within seven 7 years after delivery of a J series rotor to the original buyer by Beckman Coulter Inc or by an authorized represen tative provided that investigation and factory inspection by Beckman Coulter discloses that such defect developed under normal and proper use Should a Beckman Coulter centrifuge be damaged due to a failure of a rotor covered by this war ranty Beckman Coulter will supply free of charge all centri fuge parts required for repair REPLACEMENT Any product claimed to be defective must if requested by Beckman Coulter be returned to the factory transportation charges prepaid and will be returned to Buyer with the trans portation charges collect unless the product is found to be defective in which case Beckman Coulter will pay all trans portation charges A defective rotor will be replaced by Beckman Coulter at its then current list price less a credit based upon the age of the rotor years since date of purchase The Buyer shall not receive credit until the claimed defective rotor is returned to Beckman Coulter s Palo Alto California facility or delivered to a Beckman Coulter Field Service representative The replacement price cost to Buyer for the respective rotor shall be calculated as follows Replacement price Current rotor list price x years 7 CONDITIONS 1 Except as otherwise specifically prov
9. grams Number of buckets 12 ga Fuere eer eee oie a oda PE 4 Available labwate 223 0s ceadseecneoe 2 sees REPE see Tables 1 and 2 Approximate acceleration time to maximum speed fully loaded ec etre Se LR coe te e IER qe Rie 2 min Approximate deceleration time from maximum speed fully loaded ess ree Pr Re eR UE DER EPPRURES 1 3 4 min Weight of fully loaded rotor sees ees ess 14 03 kg 30 91 Ib Rotor material sees REA RIP LER PIQUE REESE aluminum The critical speed range is the range of speeds over which the rotor shifts so as to rotate about its center of mass Passing through or running at the critical speed range is characterized by some vibration Relative Centrifugal Field RCF is the ratio of the centrifugal acceleration at a specified radius and speed r to the standard acceleration of gravity g according to the following formula RCF 2 amp where r is the radius in millimeters is the angular velocity in radians per second 2 z RPM 60 and g is the standard acceleration of gravity 9807 mm s After substitution UM RCF 1 127 e i x t Check manufacturer s specifications for maximum allowed g force of bottles JS 5 3 Rotor DESCRIPTION This rotor has been manufactured in an NSAI registered ISO 9001 or 9002 facility for use with the specified Beckman Coulter centrifuges The JS 5 3 rated for 5300 rpm is a four place swinging bucket rotor used in Beckman Coulter Av
10. on top of the hub teeth LOADING THE BUCKETS JS 5 3 Rotor N WARNING Securely tighten the tie down knob to attach the rotor yoke to the centrifuge drive spindle hub If the rotor is left in the centrifuge between runs make sure the rotor is seated on the drive hub and the tie down knob is tight before each run gt NOTE Do not remove the tie down knob from the rotor For runs at other than room temperature refrigerate or warm the rotor and precool the centrifuge beforehand for fast equilibration N WARNING Handle body fluids with care because they can transmit disease No known test offers complete assurance that such fluids are free of micro organisms Some of the most virulent Hepatitis B and C viruses HIV I V atypical mycobacteria and certain systemic fungi further emphasize the need for aerosol protection Handle other infec tious samples according to good laboratory procedures and methods to prevent spread of disease Because spills may generate aerosols observe proper safety precautions for aerosol containment Do not run toxic pathogenic or other hazardous materials in this rotor with out taking all appropriate safety precautions Biosafe containment should be used when Risk Group II materials as identified in the World Health Organization Laboratory Biosafety Manual are handled materials of a higher group require more than one level of protection JS 5 3 Rotor
11. with one hand and with the other hand snap one latch down Then snap the second latch down Be careful not to pinch your fingers as you fasten the latches 5 Look at the bucket cover interface checking to make sure that the red O ring is not protruding at the top of the bucket If you can see a portion of the O ring above the bucket top edge release the latches and remove the cover Then repeat steps 3 and 4 to reseal the assembly JS 5 3 Rotor OPERATION Temperatures may vary slightly between centrifuges If sample temperature is crucial test temperature settings on your instrument using water samples For runs at other than room temperature refrigerate or warm the rotor beforehand for fast equilibration 1 Refer to the instrument instruction manual for centrifuge operation 2 See RUN SPEEDS page 15 for information about speed limitations REMOVAL AND SAMPLE RECOVERY N CAUTION If disassembly reveals evidence of leakage you should assume that some fluid escaped the rotor Apply appropriate decontamination procedures to the centrifuge and accessories Covers can be removed while the buckets are in the centrifuge or buckets can be placed on a benchtop before the covers are removed If hazardous materials have been centrifuged place the buckets under an appropriate hood or biological safety cabinet before removing the covers To remove covers while the buckets are in the centrifuge 1 Carefully release the lat
12. 56983 to be placed at bottom of adapter Cannot be used with bucket cover f Use this adapter inside the 250 mL adapter 392077 to run tubes at 6670 x g JS 5 3 Rotor 17 JS 5 3 Rotor Table 2 Tube and Bottle Adapters for the JS 5 3 Rotor continued each Part Maximum RCF at Number Tube Bottle Tubes Bottles Max Speed Pkg 4 Size per Adapter RODI orange 392079 250 mL conical 1 6870 x g yellow 392077 250 mL round 1 6670 x g 230 mL conical blue 392078 500 mL conical 1 6870 x g ies yellow 356966 50 mL conical 1 6670 x g each yellow 356964 15 mL conical 4 6670 x g Maximum RCF for adapters Use manufacturers recommendations for tube and bottle limits P Observe limitations described on pages 11 and 12 when using bucket covers with these adapters Maximum RCF for labware in this adapter is 4000 x g g Requires a cushion 356983 to be placed at bottom of adapter Cannot be used with bucket cover f Use this adapter inside the 250 mL adapter 392077 to run tubes at 6670 x g 18 Table 3 Relative Centrifugal Fields for the JS 5 3 Rotor Entries in the table are calculated from the formula RCF 1 12r RPM I000Y and then rounded to three significant digits JS 5 3 Rotor Deep well Plates 500 mL Conical Bottles Relative Centrifugal Field x g Relative Cent
13. J TB 089D E November 2005 JS 5 3 ROTOR Used in Beckman Coulter Avanti J 20 and J 26 Series and Avanti J E Centrifuges 2005 Beckman Coulter Inc Printed in the U S A JS 5 3 Rotor SAFETY NOTICE This safety notice summarizes information basic to the safe use of the rotor described in this manual The international symbol displayed above is a reminder to the user that all safety instructions should be read and understood before operation or maintenance of this equipment is attempted When you see the symbol on other pages of this publication pay special attention to the safety information presented Observance of safety precautions will also help to avoid actions that could damage or adversely affect the performance of the rotor A Sila ie e E Handle body fluids with care because they can transmit disease No known test offers complete assurance that such fluids are free of micro organisms Some of the most virulent Hepatitis B and C viruses HIV I V atypical mycobacteria and certain systemic fungi further emphasize the need for aerosol protection Handle other infectious samples according to good laboratory procedures and methods to prevent spread of disease Because spills may generate aerosols observe proper safety precautions for aerosol contain ment Do not run toxic pathogenic or radioactive materials in this rotor without taking appropriate safety precautions B
14. O Printed on recycled paper
15. and out of the bucket to minimize the effects of vacuum conditions inside the centrifuge during operation The covers are held in place by attached latches Refer to the Warranty at the back of this manual for warranty information Radel is a registered trademark of Solvay Advanced Polymers LLC Validation of microbiological containment was done at an independent third party testing facility Health Protection Agency Porton Down Improper use or maintenance may affect seal integrity and thus containment JS 5 3 Rotor PREPARATION AND USE Specific information about the JS 5 3 rotor is given here Use the J Series Rotors and Tubes Manual JR IM along with this rotor manual for complete rotor and accessory operation gt NOTE Although rotor components and accessories made by other manufacturers may fit in the JS 5 3 rotor their safety in this rotor cannot be ascertained by Beckman Coulter Use of other manufacturers components or accessories in the JS 5 3 rotor may void the rotor warranty and should be prohibited by your laboratory safety officer Only the components and accessories listed in this publication should be used in this rotor PRERUN SAFETY CHECKS Read the SAFETY NOTICE page at the front of this manual before using the rotor A 1 Make sure that the rotor and buckets are clean and show no signs of corrosion or cracking If any evidence of damage is present do not centrifuge the rotor 2 Check the chemi
16. anti J 20 and J 26 series and Avanti J E centrifuges Each rotor bucket can carry a 96 well kit for high throughput processing such as a DNA or RNA kit or standard microplates used in the serial dilution of small liquid volumes up to six stacked not to exceed 83 2 mm 3 28 in 96 well polypropylene plates two stacked deep well plates or one square well plate per bucket An array of adapters carry a wide range of tube and bottle sizes from 1 5 to 500 mL The rotor yoke is made of aluminum and is black anodized for corro sion protection Blue anodized aluminum buckets can be run by placing them over pivot pins on the arms of the yoke they swing out to horizontal position during centrifugation A tie down knob secures the rotor to the centrifuge drive hub Aeroseal covers made of transparent Radel are available These covers have been tested to demonstrate containment of microbiolog ical aerosols under normal conditions of the associated Beckman Coulter rotors and centrifuges when used and maintained as instructed In the event of labware leakage the covers will contain liquids and broken labware reducing the need to clean the centrifuge chamber and allowing you to take appropriate precautions before removing the covers Each cover requires a replaceable silicone rubber O ring that seats around the outside edge of the cover An air vent filter allows passage of air but not of liquids or aerosols larger than 0 3 micron in
17. ath the cover with a pencil or other non metal tool that will not scratch the cover material Insert a new air vent filter into each cover An audible snap will be heard as the filter is inserted When properly inserted the filter will sit slightly 0 76 mm above the cover surface Approximately once a week and after cleaning and or auto claving wipe the rotor pins and pin sockets see Figure 7 with a paper towel then coat pin sockets with Paint On Graphite Lubri cant 977212 Allow the lubricant to dry for at least 5 minutes before installing the rotor in a centrifuge e Apply a light film of powder such as talcum powder to the tube adapter rubber bases after cleaning or as required to prevent sticking Store the rotor in a dry environment not in the centrifuge Refer to Appendix A in Rotors and Tubes for the chemical compatibilities of rotor and accessory materials Your Beckman Coulter representa tive provides contact with the Field Rotor Inspection Program and the rotor repair center CLEANING M Rotor Cleaning Kit 339558 JS 5 3 Rotor Lubrication Area both pin sockets all buckets Figure 7 Bucket Lubrication Area Wash rotor components immediately if salts or other corrosive materials are used or if spillage has occurred Do not allow corrosive materials to dry on the rotor Under normal use wash the rotor frequently at least weekly to prevent buildup of residues I gt NOTE Do not wash
18. cal compatibilities of all materials used Refer to Appendix A in Rotors and Tubes 3 Verify that the labware being used is listed in Table 1 or Table 2 INSTALLING THE ROTOR YOKE Two metal drive pins inside the rotor drive hole engage with teeth on the centrifuge hub to prevent the rotor from slipping during accel eration and deceleration The drive pins are positioned parallel to the rotor yoke arm on which BECKMAN COULTER is engraved see Figure 1 Note the drive pin orientation as you follow the steps below JS 5 3 Rotor Figure 1 Rotor Drive Pin Location and Orientation 1 Rotate the yoke until the pins are either parallel to or perpen dicular to the drive spindle hub teeth The drive pins may rest either next to or between the drive hub teeth 2 Carefully lower the rotor straight down onto the centrifuge drive hub N CAUTION Carefully lower the rotor yoke straight down onto the drive spindle hub to avoid bending the drive spindle hub assembly Do not drop the rotor onto the hub 3 Slowly rotate the rotor yoke to make sure it is properly seated In rare cases the rotor pins may rest on top of the hub teeth preventing the yoke from being secured to the drive hub Turning the yoke slightly after installing it will ensure that the rotor is correctly seated Lift the rotor yoke slightly while turning it 4 Tighten the tie down knob The tie down knob will not engage with the drive hub if the drive pins are
19. ches one at a time keeping the bucket stable with one hand Alternately place your thumb and or fingers on the edge of the cover and lift up A number of different hand positions can be used Find one that is comfortable and provides adequate leverage See the Note below if you cannot remove a COVer 2 Gently lift up one of the latches to break the seal formed during centrifugation 3 Remove the cover being careful not to disturb the bucket contents 4 Remove the labware from the bucket 13 JS 5 3 Rotor To remove covers from buckets placed on a benchtop 1 Place an absorbent pad on the benchtop to increase bucket stability on the benchtop surface 2 Remove a bucket from the rotor and place the bucket on the pad 3 Carefully release the latches one at a time Lift up on one latch to break the seal See the Note below if you cannot remove a cover 4 Remove the cover being careful not to disturb the bucket contents 5 Remove the labware from the buckets 6 If removing the rotor yoke loosen the tie down knob and lift the yoke straight up and off the drive hub I gt NOTE If a cover is difficult to remove wait to 2 minutes then try again to lift the cover off the bucket If this doesn t work try changing hand positions and lifting again If required use a plastic spatula to lift the cover off the bucket Do not use a metal tool that could damage the O ring or bucket To prevent covers from sticking
20. d assume that some fluid escaped the rotor Apply appropriate decontamination procedures to the centrifuge and accessories Never exceed the maximum rated speed of the rotor and labware in use Refer to the section on RUN SPEEDS and derate the run speed as appropriate Do not use sharp tools on the rotor that could cause scratches in the rotor surface Corrosion begins in scratches and may open fissures in the rotor with continued use JS 5 3 Rotor JS 5 3 ROTOR Min eu min Tay 1 Axis of Rotation Tav Tmax Tmax Axis of Rotation SPECIFICATIONS Maximum speed ocv on cove nes REM LERRA eae kbs RS 5300 rpm Critical speed range as ac0geee ode mei tie ioiii RP AES ERR 500 to 1200 rpm Density rating at maximum speed 00000000005 1 2 g mL Relative Centrifugal Field at maximum speed Deep well Plates 500 mL Conical Bottles At Tmax 194 8 mm 6130x g s sus 218 4 mm 6870 x g At ray 179 6 mm 5650 X g leues 155 6 mm 4900 x g At Fmi 164 3 mm 5170 X g 0 eee 92 7 mm 2920 x g Conditions requiring speed reductions see RUN SPEEDS k factor Deep well plates sis ccsnt ogy seus wien DERE db igen 1536 500 mL conical bottles i pcs seen head ane es ede eee RE ER pue 7728 Maximum allowable imbalance of opposing loads 10 grams Maximum load per bucket 00 0002 eee eee 1040
21. disinfection is expressed or implied When sterilization or disinfec tion is a concern consult your laboratory safety officer regarding proper methods to use When it is not in use store the rotor in a dry environment not in the centrifuge Before returning a rotor or accessory for any reason prior permission a Returned Goods Authorization form must be obtained from Beckman Coulter Inc This RGA form which may be obtained from your local Beckman Coulter sales office should contain the following information rotor serial number history of use approximate frequency of use reason for the return original purchase order number billing number and shipping number if possible name and phone number of the person to be notified upon receipt of the rotor or accessory at the factory and name and phone number of the person to be notified about repair costs etc To protect our personnel it is the customer s responsibility to ensure that the parts are free from pathogens and or radioactivity Steriliza tion and decontamination must be done before returning the parts Smaller items such as tubes bottles etc should be enclosed in a sealed plastic bag All parts must be accompanied by a note plainly visible on the out side of the box or bag stating that they are safe to handle and that they are not contaminated with pathogens or radioactivity Failure to SUPPLY LIST JS 5 3 Rotor attach this notificat
22. do the following between runs e Lubricate the O rings with a thin coat of silicone vacuum grease 335148 Inspect the air vent filters before each run to make sure that they are clean If a filter is discolored replace it LABWARE Q Temperature Limits E Beckman Coulter plastic adapters and carriers have been centrifuge ee tested for use at temperatures between 2 and 40 C For p centrifugation at other temperatures pretest labware under B anticipated run conditions 14 RUN SPEEDS JS 5 3 Rotor If plastic containers are frozen before use make sure that they are thawed to at least 2 C prior to centrifugation Labware Use the labware listed in Table 1 or Table 2 in the buckets gt NOTE RCF limits in Table 2 are the tested limits for the adapters only Tubes and bottles used may require lower run speeds Refer to manufac turers recommendations and test labware before use The centrifugal force at a given radius in a rotor is a function of speed Comparisons of forces between different rotors are made by comparing the rotors relative centrifugal fields rcf When rotational speed is adjusted so that identical samples are subjected to the same rcf in two different rotors the samples are subjected to the same force see Table 3 for examples If the weight of the load in a bucket exceeds 1040 grams or if the solution density is more than 1 2 g mL reduce the maximum allow able run speed
23. erile 267005 pkg 12 aluminum foil lid 538619 pkg 100 deep well polypropylene plate 96 well 1 mL well 267007 cap strip nonsterile 267002 pkg 102 sterile pkg 24 l cap strip sterile 267005 pkg 12 aluminum foil lid 538619 pkg 100 square well polypropylene plate 2 mL well 140504 aluminum foil lid 538619 pkg 100 pkg 24 When stacking polypropylene multiwell plates place a support pad 369382 beneath the bottom plate and a cap strip between the plates to prevent breakage during centrifugation Also use the support pad beneath all polystyrene deep well plates T Caps are optional Requires 4 inch soft rubber roller 538618 for installation 16 Table 2 Tube and Bottle Adapters for the JS 5 3 Rotor Part Maximum RCF at Number Tube Bottle Tubes Bottles Max Speed Adapter Pkg 4 Size per Adapter ROB beige 392071 13 mm dia 33 6145 x g purple 392072 16 mm dia 24 6145 x g red 392073 17 mm dia 20 6145 x g yellow 392074 29 mm dia 8 6145x g green 392075 15 mL conical 18 6425 x g black 392076 50 mL conical 7 6480 x g Maximum RCF for adapters Use manufacturers recommendations for tube and bottle limits Continued P Observe limitations described on pages 11 and 12 when using bucket covers with these adapters Maximum RCF for labware in this adapter is 4000 x g d Requires a cushion 3
24. he yoke by aligning the grooves in the bucket sides with the pivot pins then sliding the buckets down until the pivot pins are seated in the bucket pockets 5 Gently swing the buckets to ensure that they are properly seated on the pivot pins Using Covers gt NOTE Covers can be used only with blue buckets 368706 They cannot be used with previously manufactured black buckets 368415 Always visually check to make sure that the labware fits under the cover before your attempt to secure the latches In addition note the following information for specific components When using six stacked polystyrene microplates make sure that the plates are aligned parallel to the bucket edge as shown in Figure 3 before placing a cover on the bucket G 3 G OO0000000009 OOOOOOOOOOOO OOOOOOOOOQO0O O60000000000 0909000000000 QOOOCOCOOO0CO OOOOOOOOOO000 OOOO000000000 OOOO0O00000000 O OOO000000000 OOO0000000000 OOOO0O00000000 OOO0O000000000 OOO000000000 OOOO0O00000000 O 0000000000 6 Goa Correct Incorrect Figure 3 Correct and Incorrect Alignment of Microplates in the Bucket JS 5 3 Rotor All adapters listed in Table 2 can be used with covers except 500 mL conical adapter 392078 Adapters 392071 392072 and 392074 have the following tube height limits In adapter 392071 the maximum height of tubes placed in the four corner positions of the adapter sh
25. ided herein this war ranty covers the rotor only and Beckman Coulter shall not be liable for damage to accessories or ancillary supplies including but not limited to i tubes ii tube caps iii tube adapters or iv tube contents 2 This warranty is void if the rotor has been subjected to customer misuse such as operation or maintenance con trary to the instructions in the Beckman Coulter rotor or centrifuge manual 3 This warranty is void if the rotor is operated with a rotor drive unit or in a centrifuge unmatched to the rotor charac teristics or is operated in a Beckman Coulter centrifuge that has been improperly disassembled repaired or modified 4 Each bucket whether purchased with a rotor assembly or purchased separately is covered by this warranty for seven 7 years from the date of purchase and will be replaced or repaired during such period according to the terms and conditions of this warranty The date of manufacture marked on the bucket may be earlier than the date of pur chase and the expiration date marked on the bucket which is seven 7 years after the date of purchase may be correspondingly offset 5 Buckets should not be used after the expiration date marked on the bucket If at the time of purchase the marked expiration date is less than 7 years from the date of purchase the expiration date becomes the date of purchase plus seven 7 years Use of a bucket after such expiration date voids Beckman
26. ion will result in return or disposal of the items without review of the reported problem Use the address label printed on the RGA form when mailing the rotor and or accessories to Beckman Coulter Inc 1050 Page Mill Road Palo Alto CA 94304 Attention Returned Goods Customers located outside the United States should contact their local Beckman Coulter office gt NOTE To obtain copies of referenced publications contact Beckman Coulter Inc Technical Publi cations Department 1050 Page Mill Road Palo Alto CA 94304 U S A telephone 650 859 1753 fax 650 859 1375 Contact Beckman Coulter Sales 1 800 742 2345 in the United States or your local Beckman Coulter office or see the Beckman Coulter High Performance High Speed High Capacity Rotors Tubes and Accessories catalog BR 8102 available at www beckmancoulter com for detailed information on ordering parts and supplies For your convenience a partial list is given below REPLACEMENT ROTOR PARTS JS 5 3 rotor assembly ss dearer esr nreno nei a eee eee eee 368690 Rotor tie down knob ureri eee eee nee 368410 Bucket blue set of 4 0 0 0 0 0c cc eee nee 368706 Containment cover set includes 2 covers with pre installed O rings and air vent filters plus 2 each replacement O rings and filters 2 he kat pv Sua Glas dece 368417 O ring replacement set qty 4 0 eccere eee eee 368703 Air vent filter replacement set qty 60 ll
27. iosafe containment should be used when Risk Group II materials as identified in the World Health Organization Laboratory Biosafety Manual are handled materials of a higher group require more than one level of protection This rotor was developed manufactured and tested for safety and reliability as part of a Beckman Coulter centrifuge rotor system Its safety or reliability cannot be assured if used in acentrifuge not of Beckman Coulter s manufacture or in a Beckman Coulter centrifuge that has been modified without Beckman Coulter s approval Although rotor components and accessories made by other manufacturers may fit in the JS 5 3 rotor their safety in this rotor cannot be ascertained by Beckman Coulter Use of other manufacturers components or accessories in the JS 5 3 rotor may void the rotor warranty and should be prohibited by your laboratory safety officer Only the components and accessories listed in this publication should be used in this rotor Hook all four buckets loaded or empty to the rotor for every run Make sure that filled containers are loaded symmetrically into the rotor and that opposing labware is filled to the same level with liquid of the same density The rotor and accessories are not designed for use with materials capable of developing flammable or explosive vapors Do not centrifuge such materials in nor handle or store them near the ultracentrifuge If disassembly reveals evidence of leakage you shoul
28. le 368148 Microplate carrier Kats oc ess as ee niieg ei eae EE 368914 25 JS 5 3 Rotor OTHER 26 Contains Microplate carrier set of 4 2 0 00 0 00 c eee eee eee 368905 Support pad set of 4 sis maisi ea eee eee eee eens 369382 Wea Wale RR MT LIEN see Tables 1 and 2 Replacement tube and bottle adapters set of 2 Beige 13 mm dia oce rr eter ep over akan s 368907 Purple 16 mim dia osse REIR RR RR eve 368909 Red 17 mm dia iuo d aces Se 8 ae ah 368910 Yellow 20 mim dia i es Sd RR RE RES 368911 Green 15 mL conical ereis sererai eae gna Earr a 368915 Black 50 mL cortical 22s bee baton ia E s 368916 Orange 250 mL conical spriin eee eee 369385 Yellow 250 mL round 230 mL conical 000 369383 Blue 500 mL conical 0 0 0 0 0 0 e e a ANNS 369384 Rubber roller 4 in for sealing foil microplate lids 538618 Rotor Cleaning Kif i i eos ee er 84 REL RRR eee 339558 Beckman Solution 555 1 qt 0 eee eee ee eee 339555 Rotor cleaning br sh 12 a4 650 ng Rig ee aes weed ee 339379 Paint On Graphite Lubricant 2 02 000c cece eee eeee 977212 Silicone vacuum grease 2 0Z 1 0 0 cece eee eee eee 335148 J SERIES SWINGING BUCKET ROTOR WARRANTY Subject to the conditions specified below and the warranty clause of the Beckman Coulter Inc terms and conditions of sale in effect at the time of sale Beckman Coulter Inc agrees to correct either by repair or
29. osing buckets should contain similar loads see Figure 2 Using Buckets You can load buckets before or after they are installed on the rotor yoke In either case we recommend filling the appropriate labware first and then loading the labware into the buckets to avoid tripping the imbalance detector gt NOTE When using stacked polypropylene microplates place a support pad 369382 beneath the bottom plate and place a cap strip between plates to prevent breakage during centrifugation Use the support pad beneath all polystyrene plates JS 5 3 Rotor Cx OM XK MK OOOOOOOOOOOO OOOOOOOOOCOCOCO 000000000008 eeooooooooee eeooooooooee eeooooooooee o o O Q e e e e 00000000 00000000 00000000 Example of Loaded Tray Examples of Symmetrically Loaded Adapters Examples of Symmetrically Loaded Trays Load Opposite Trays the Same Way 000000800000 000000000000 OOOOOOOOOOOO D Nonsymmetrically Figure 2 Symmetrical and Balanced Loading All four positions must have a bucket attached for every run JS 5 3 Rotor Support Pad Microplate Carrier 10 1 Insert the filled labware into the adapter or carrier See page 14 for labware information 2 Load the filled adapters or carriers into the buckets 3 If using bucket covers follow the procedure below under Using Covers 4 Attach each bucket to t
30. own shaded in Figure 4 is 90 mm Tubes up to 105 4 mm tall can be placed in all other positions Figure 4 Tube Height Limits for Certain Tube Positions in Adapter 392071 In adapter 392072 the maximum height of tubes placed in the end positions of the middle row shown shaded in Figure 5 is 104 mm Tubes up to 114 mm tall can be placed in all other positions Figure 5 Tube Height Limits for Certain Tube Positions in Adapter 392072 In adapter 392074 capped tubes placed in the four corner posi tions must be oriented so that the cap hinges and tabs do not extend towards the corners of the adapter see Figure 6 11 JS 5 3 Rotor 12 Leave Clear Figure 6 Orientation of Capped Tubes in Corner Positions of Adapter 392074 Install the covers as follows N CAUTION When centrifuging hazardous materials load and unload buckets and install and remove Aeroseal covers under an appropriate hood or biological safety cabinet 1 Make sure that the bucket and bucket cover surfaces are clean dry and undamaged 2 Make sure that the O ring 368703 is in good condition and lightly coated with silicone vacuum grease 335148 3 Hold the cover with both hands holding the latches up and place the cover on the bucket Push down on all four corners at once to properly seat the cover Look at the bucket from the side to make sure that the cover is centered on the bucket 4 Steady the bucket
31. rifugal Field x g Rotor Rotor Speed At Imax At fay At fin Speed At Imax At fay At fin rpm 194 8 mm 179 6 mm 164 3 mm rpm 218 4 mm 155 6 mm 92 7 mm 5300 6130 5650 5170 5300 6870 4900 2920 5000 5450 5030 4600 5000 6120 4360 2600 4750 4920 4540 4150 4750 5520 3930 2340 4500 4420 4070 3730 4500 4950 3530 2200 4250 3940 3630 3320 4250 4420 3150 1880 4000 3490 3220 2940 4000 3910 2790 1660 3750 3070 2830 2590 3750 3440 2450 1460 3500 2670 2460 2250 3500 3000 2140 1270 3250 2300 2130 1940 3250 2590 1840 1100 3000 1960 1810 1660 3000 2200 1570 934 2750 1650 1520 1400 2750 1850 1320 785 2500 1360 1260 1150 2500 1530 1090 649 2250 1110 1020 932 2250 1240 882 526 2000 873 805 736 2000 978 697 415 1750 668 616 564 1750 749 534 318 1500 491 453 414 1500 550 392 234 1000 218 201 184 1000 245 174 104 750 115 103 108 750 138 98 58 500 55 50 46 0 0 0 0 Refer to manufacturers recommendations for maximum RCF limits of bottles 6 500 6 000 5 500 5 000 4 500 4 000 3 500 RCF x g 3 000 2 500 2 000 1500 1000 500 0 Relative Centrifugal Fields for the JS 5 3 Rotor with Deep well Plates 0 1000 2 000 3 000 Speed rpm 4 000 5000 5300 max m m min Relative Centrifugal Fields for the JS 5 3 Rotor with 500 mL Conical Bottles 8 000 7 000 6 000 5 000 4 000 3 000 2 000 1000 0 RCF x g
32. te France 95942 ROISSY CDG Cedex Telephone 01 49 90 90 00 Fax 01 49 90 90 10 e mail bioresearch france 2beckman com GERMANY Beckman Coulter GmbH Europark Fichtenhain B 13 47807 Krefeld Germany Telephone 49 21 513335 Fax 49 21 51333633 e mail bioresearch de 2 beckman com O zeoe COULTER Beckman Coulter Inc Sales and Service 1 800 742 2345 HONG KONG Beckman Coulter Hong Kong Ltd 12th Floor Oxford House 979 King s Road Taikoo Place Hong Kong Telephone 852 2814 7431 2814 0481 Fax 852 2873 4511 ITALY Beckman Coulter S p a Centro Direzionale Lombardo Palazzo F 1 Via Roma 108 20060 Cassina de Pecchi Milano Italy Telephone 02 953921 Fax 02 95392264 JAPAN Beckman Coulter K K Toranomon 37 Mori Bldg 3 5 1 Toranomon Minato ku Tokyo 105 0001 Japan Telephone 03 5404 8359 Fax 03 5404 8436 MEXICO Beckman Coulter de Mexico S A DE C V Avenida Popocat petl 396 Colonia Gral Pedro Maria Anaya Codigo Postal 03340 Mexico D F Mexico Telephone 525 605 77 70 Fax 525 575 18 25 NETHERLANDS Beckman Coulter Nederland B V Nijverheidsweg 21 3641 RP Mijdrecht Postbus 47 3640 AA Mijdrecht The Netherlands Telephone 0297 230630 Fax 0297 288082 SINGAPORE Beckman Coulter Singapore Pte Ltd 116 Changi Road Unit 03 01 02 Singapore 419718 Telephone 65 6339 3633 Fax 65 6336 6303 SOUTH AFRICA SUB SAHARAN AFRICA Beckman Coulter Stand 1A
33. the rotor components in a dish washer Do not soak the rotor in detergent solution for long periods such as overnight 1 Wash the rotor yoke buckets adapters and microplate carriers in a mild detergent such as Beckman Solution 555 339555 that won t damage the rotor The Rotor Cleaning Kit 339558 contains two plastic coated brushes and two quarts of Solution 555 for use with rotors and accessories Dilute the detergent 10 to 1 with water 2 Thoroughly rinse the cleaned rotor components with distilled water 3 Air dry the rotor components upside down Do not use acetone to dry the rotor 21 JS 5 3 Rotor 4 When adapters are dry apply a light film of powder such as talcum powder to the rubber bases to prevent sticking 5 When the rotor is dry lubricate the bucket sockets and pins as described under MAINTENANCE Cleaning Bucket Covers The covers and O rings should be cleaned at least weekly under nor mal use Remove and discard the filter before cleaning Insert a new filter after cleaning and before the next use 1 Remove the air vent filters as described in MAINTENANCE above and O rings from the covers see Figure 8 2 Wash the covers and O rings with a mild detergent such as Beckman Solution 555 339555 diluted 10 to 1 with water Do not wash the components in a dishwasher 3 Thoroughly rinse the cleaned components with water and air dry upside down Do not use acetone to dry the components 4
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