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UM-AJV5-01-1060

1. 238 200 500 9 39 12 9 fl 3 9 11 1 418 x 71 9 Fluidic Schematics Abacus junior FLUIDIC SCHEMATICS VERSION 1 04 Puttar he 16 Wor z 1 LX v1 DrainChamber T vs Liquid Detector 2 way Valve WASTE Of Valve n Stepper Moor Dpan 1 3 2 3 On 12 10 Warning labels on the analyzer Biohazard The sample and the waste potentially infectious material Corrosive Reagents may cause corrosion or skin irritation Warning General warning of injury Sharp The sampling needle may needle cause injury warning Table 6 Warning labels 73
2. 5 This will be the header of the printouts Press to accept the settings User mode settings If you want to require users to log and enter passwords to use cAbacus junior vet 5 and to be able to track individual usage of the unit you can enable the Abacus junior vet 5 s Multi user mode This will require each user to have a unique user ID and password 1 Press the Utilities key 1 General Settings 2 Select Settings 2 Units 3 Laboratory 3 Select Customize 4 User modes 4 Select User mode User modes 5 Follow the on screen instructions to a Single user made enable multi user mode to add or O2 Multi user made modify users or to change a 5 password 7 5 4 Date and time The date and time of each analysis is stored with the results This menu allows setting the built in clock and the format of the date displayed 1 Press the Utilities key and then select Tateandtime Settings LER date and time 2 Select Date and time amp 2 Dau Month ear 3 Select Set date and time 23 Month Day Year Od Year Honth Dau 5 24 hour format OG 12 hour format 4 Type in the date and time then press Lv The current date and time are 15 Hb z HT 14 52 5 Select formats for displaying the date item 2 3 or 4 and time item 5 or 6 then press BER set date and time amp 2 Oo Honth D au Tear Od Year Honth Dau 5 24 hour
3. 18 PN EU RIEN 18 Dede TET MOMS ACL ORS 18 DOM Electicalreguire Meni 18 PA ACS 0S E T 19 PAS CTC AUS 19 2 244 Reagents and waste handling Gu ios 20 Dede ____________ 20 E __ _______ __ 20 PAL TEE 20 2 9 Unpacking and installation roa ene eek bs buda poor e 21 23L Turine ihe 23 2 3 2 Turning the Instrument OFF e 24 259 9 PRC ALINE VOR _ __ __ 25 2 94 COC NI _ ___ 25 ye o erem 26 Sel CIENT Umm 26 3 1 1 Navigating in Menu 26 5 gt 28 qd OPERATING PRINCIPLES 33 AA Impedance Method esos ____________ __ _ 33 42 Prnciple or HOB Measurement dust ena uoc comae oes 33 Ro cH s E TT OO OD Emm 34 4 4 Absolute and Linearity Ranges of Measured 35 5 ROUTINE UTILIZATION and MEASURE V9 ee eee e eee eee eee eee eee eaae eee 36 2 NIN e 36 TEE 39 DEPE Par O e 39 5 2 2 Modifying lyse quantity 39
4. a You can select a menu item move highlight with the keys and press the OK key to enter or activate the highlighted item Within a submenu you can press the function key to return to the previous menu level This method is suggested while learning instrument operation b Pressing the numeric key corresponding to the desired menu item allows selection and access of an item without the need to additionally press the OK key Pressing the 0 zero key has the same effect as the function key This method is more efficient when the user knows the menu structure You can also move between the different menu levels using the lt and keys These have the same effect as OK and respectively If a menu item would open up a submenu then the item is indicated with a symbol on the right of the menu line some results can be displayed in table format The following keys may be used for browsing the database 31 91 page down 11 jump to top of list 74 jump to bottom of list Several menus have items with boxes in front of the text These indicate two state options The selected active state is indicated with a filled box the deselected inactive state is indicated with an empty box Pressing OK toggles its state Other items have circles in front of the text These are called radio buttons They are divided into groups separated by horizontal lines The function of these groups is
5. Diluent Solution Cleaner Figure 5 Reagent connections WARNING Reagents may cause corrosion and skin irritation If any of liquids leaked A to cover of analyzer or the furniture it has to be wiped down immediately In case of skin contact the liquid has to be rinsed by plenty of water 2 3 1 Turning the Instrument a In case you use an external printer for information read manual shipped with the printer connect it and turn it on b Turn the ceAbacus junior vet 5 on using the power switch on the rear panel The On position is marked by the 1 symbol Abacus junior vet 12 06 2007 12 06 2007 12 06 2007 12 06 2007 12 88 2887 During start up the following screen is displayed The software version number appears few seconds later when the software starts An important feature of the instrument is that when the software version is completed the DATABASE will be displayed without any pneumatic initialization default setting Pneumatic movement will be initiated only when necessary for the relating process The default setting can be changed at Service Menu level in this case the instrument will start with pneumatic initialization giving the possibility to perform a measuring process immediately Please the Service Personnel in case you want to change the setting CAUTION 5 minutes before initiating any measuring process to allow the instrument to reach
6. Command Go to specified record Jumps to a particular sample record Enter the date and time sample ID and patient ID of the sample you want to view and press __ The first sample meeting your parameters is then displayed If you leave any ID blank 0 records are searched by date time only Selects all sample records in memory or all having a specific date time and ID Select by date time and ID allows you to select a range of specific records and Deselects all deselects all records Entering 0 as an ID searcehs by date time only Corresponding results are marked with a filled box Change sort order Changes the order in which results are displayed by time sample ID or patient ID Manage selected Sends selected records to a PC deletes them or saves records them to a diskette or USB device Before selecting Backup selected records insert a 3 5 inch diskette or connect a USB storage device An empty floppy diskette can store data for 800 samples View external Views previously saved data from a diskette or USB storage device Backup one day Backs up all records from a specified day to a diskette or USB storage device Select a day to backup then press to confirm 48 UTILITIES 7 UTILITIES 7 1 Maintenance By selecting item 1 of the UTILITIES you can access the MAINTENANCE menu UB l lainitenance Calibration 3 Quality control 4 Diagnostics 5 Settings Service 7 1 14 Regular Mai
7. DD date MM month year Set both instruments to the same format 7 5 3 Units Use the unit menu to set the measurement units the Abacus junior vet 5 will use 1 Press the Utilities key 9 2 Select Settings 3 Select Customize 4 Select Units 5 Set the units as needed Use the lt and gt keys to select a unit B Use the and V keys to select a setting that unit 1 Maintenance 2 Calibration Guality control Diagnostics 3 4 5 gaervice Customize L General Settings Units Count unit HGE writ urit made 66 Table 3 5 lists the available unit settings Table 3 5 Units Count unit ceallsjiter celle cells ul Prec displays a more precise value HGB unit grams liter cT grams deciliter adl millimolsliter mmol T PCT HCT unit percentage 5 absolute abs ACW POW mode standard deviation sd coefficient of variation ev 6 Press the on screen key to save the settings Laboratory information settings Enter your clinic or laboratory information as follows to be printed automatically on report headers You can also use this procedure to edit or change this information Enter the name and address of your clinic or laboratory Press OK to move to the next line You can enter up 40 characters in each line aet Laboratory name The of the Laboratory is
8. The Off position is marked by O symbol 24 2 3 3 Preparing for shipment The second item in the shut down menu should be used when the instrument is to be shipped or left unused for a longer time more than 2 weeks The instrument will ask you to use the cleaning tube kit and 100ml of distilled water Follow the instructions appearing on the display EXIT eoa A PREPARING FOR SHIPMENT 2 Remove reagent tubing at rear reagent inputs Diluent Lyse Lyse Vet5 Rinse Here the user is instructed to remove tubing and Cleaner Leave waste connected connectors so they could be drained Leave the waste connector attached Next you should connect the cleaning tube P 0050 kit to the reagent inputs submerging the free Connect min 100 ml distilled water to end in a bottle containing at least 100 ml of reagent inputs using cleaning tube kit distilled water Cleaning tube kit connected The analyzer will flush any remaining reagents from the system into the waste container As a next step the analyzer asks you to Message 5003 19300 remove the cleaning tube kit Remove cleaning tube kit Keep reagent inputs free Leave waste connected When finished the analyzer prompts you to power off the system Remove the waste connector after shutting down 2 3 4 Emergency handling In case of emergency situation like instrument catching on fire short circuit etc cut off power immediately and use a fire ex
9. The best place is on the ground below the supporting desk the instrument is placed on The pneumatics system is capable of aspirating reagents from containers being 1 0 m below the reagent inputs Make sure the reagent tubes are not bent broken twisted or blocked in between the desk the instrument is on and the wall behind Such circumstances can result in instrument operation failure DO NOT PLACE the reagents above the instrument as there can be a risk of falling and spilling WARNING Install the unit on a table or workbench If the unit is installed without a supporting desktop under the unit there is a possibility that 4bacus junior vet 5 could accidentally fall 2 2 3 Peripherals External peripherals should only be connected when both the instrument and the peripheral device are powered off Possible peripherals are external printer o the printer must be recommended by authorized technician o the printer must be approved and listed o the printer must have a CE mark external keyboard the external keyboard must be approved o the external keyboard must have a PS2 connector or suitable adapter link to host computer via serial or USB port o serial USB link cable must be approved by technician 19 2 2 4 Reagents and waste handling Reagents should be handled according to national or international regulations WARNING Reagents may cause corrosion and skin irritation If any of the liquids leaked onto the c
10. 11 Use Li keys on the screen to move through the results and histograms 40 The following table summarizes warning flags and give an explanation of their possible cause and a few hints to overcome the problem Uppercase letters refer to WBC or HGB problems Flag Meaning Recommended user action No WBC 3 part differential Possible lyse problem May occur pathological lymphocytosis blank is high or Repeat the blank measurement If blank is not stable HGB blank there are probably bubbles in the WBC chamber Run a cleaning and try blank again Close the side door if open during measurement WBC blank is high or no Repeat the blank measurement or run prime lyse and try WBC blank blank again Possible lyse contamination or noise problem C Q WBC clogging Aperture clogging Perform cleaning and repeat the measurement If it is a general problem please contact your Service Personnel Low temperature reagents can cause it as well mainly diluent in this case you will have to wait until they reach room temperature Table 4 Summary of warning flags related to WBC HGB Warning flags in lowercase refer to RBC or PLT problems Flag Meaning ___ Recommended user action PLT blank is high or cleaning and repeat the blank measurement PLT blank Diluent or system cleanliness problem If it is stable high replace the diluent by opening a new tank blan
11. 7 1 1 4 Reagent status Reagent status Reagent pack installed 61 61 2664 Reagent pack Blanks Duluent Lyza Cleaner L VETE Rinsa Mgzke 3000 00 00 00 11000 The screen on the left shows reagent volumes in containers as calculated by the instrument As measurements are performed the volumes are changing accordingly When reagent volume in a container is running low instrument will notify user and ask replacement Press the I a soft key then press Ito confirm The Abacus junior vet 5 updates the installation date reagent lifetime and the amount of reagent in each container 50 UTILITIES WASTE HANDLING VERY IMPORTANT Waste contains poisonous substances because of possible cyanide content and human origin substances representing biohazard These substances are representing potential danger to environment For this reason safe handling of the waste liquid is very important Please contact your distributor which kind of reagent is supplied to you whether the lyse reagent contains cyanide or it is cyanide free Please disregard point 1 below your lyse reagent does not contain cyanide The following steps should be made for environmental protection and safety reasons 1 Neutralization of waste containing cyanide Use set of cyanide neutralizer reagents contact your distributor Component A alkaline oxidant reagent Component B neutralizer reagent Put 5 of c
12. High voltage pulse on aperture in each analysis cycle chemical cleaning and high pressure back flush of the aperture using Cleaner reagent High voltage burst of the aperture high pressure back flush chemical cleaning of the aperture 6 QC levels QC parameters include mean range SD and CV for all measured and calculated parameters 16 and 64 day Levey Jennings charts separate QC database 3 measurement automatic and manual calibration of WBC HGB RBC PLT MCV RDW MPV and EOS absolute Independent calibration of pre diluted mode 3 level multi user operation with selective privilege levels user identification with ID and password Easy to use menu driven user interface with 6 software buttons with icons 6 hardware function buttons cursor and numeric keys English French Italian German Spanish Russian Hungarian 2000 results with RBC PLT WBC 3 part and EOS histogram 5 part mode USB B port or RS 232 USB mass storage device PenDriveTM Free DiatronLab data management software via USB A port using USB mass storage device PenDriveTM Canon BJC Esc P Esc P2 Esc P raster PCL4 Seiko Easy Paper Operation thermal printer 56 mm wide roll paper 240x128 dots high contrast backlit monochrome white on blue graphics LCD liquid crystal diode 29 foil keys separate START button Standard PS 2 compatible keyboard 12VDC 6A 72W max operating power External auto ranging power unit for 100 120
13. MPV values PLT x MPV x 100 percentage absolute PLT x MPV White blood cell differential Absolute values counted in the channels determined by the LYM LY lymphocytes three WBC discriminators MID MID monocytes and some eosinophils GRA GR neutrophil eosinophil and basophil granulocytes Percentages calculated from the absolute WBC value Eosinophil EOS EOS EOS shown by the second peak on the histogram 34 4 4 Absolute and Linearity Ranges of Measured Parameters The instrument is guaranteed to provide specified accuracy within its linearity range Beyond this linearity range the instrument is able to display results but may not guarantee accuracy characteristics If the value is over the maximum range of guaranteed linearity the instrument cannot measure it and the result will be marked with an E Error flag To measure a sample whose parameters exceed the maximum value indicated in the table below pre dilution is recommended See section 5 3 1 3 of this manual The linearity ranges of primary parameters in normal measuring mode Linearity Ranges Maximum Unit W 0 100 150 10 cells liter M PLT 0 700 1000 10 cells liter BC RBC PLT Table 2 Linearity ranges of parameters 0 250 400 g The linearity ranges for 1 5 pre dilution mode Linearity Ranges Maximum _ 101 cells liter WBC ida 100 2000 3000 10 cell
14. START key rotor with interchangeable adapters PUE XN CU oe Figure 2 Rear view Reagent tubing connections On Off switch External power supply inlet 12VDC USB A port Serial port RS 232 USB B port Keyboard connector PS2 The analyzer is equipped with built in printer allowing immediate report generation Abacus Juinor 5 is designed to support two printer models a Seiko and Axiohm model Both printers use 58 wide thermal roll paper Installation of the printer is a factory option Each analyzer that has the built in printer option will be equipped either with the SEIKO or with the AXIOHM printer module The SEIKO model has a flat top panel with color matching that of the instrument cover To open its lid press the black button Just let the thermal printer paper fall into the housing with its open end toward the black roller Holding the open end of the paper close the lid so that the end of the paper gets clipped between the black paper guide and the printer mechanics You can select to use this printer in the Printer Settings menu see chapter 7 5 1 Paper guide Thermal paper Printer mechanics inside EN Lid opener button Figure 3 Built in printer The AXIOHM printer has a black cover It also has a blue status light that blinks when the printer paper is out To replace paper in the printer open the paper lid pull the lid upwards by the handle remove
15. blood sample WBC total white blood cell count LYM lymphocytes count MON monocytes count NEU neutrophil count EOS eosinophil count BAS basophil count LYM lymphocyte percentage MON monocytes percentage NEU neutrophil percentage EOS eosinophil percentage BAS basophil percentage HGB hemoglobin RBC red blood cell count HCT hematocrit MCV mean corpuscular volume MCH mean corpuscular hemoglobin MCHC mean corpuscular hemoglobin concentration RDWc red cell distribution width PLT platelet count PCT platelet percentage MPV mean platelet volume PDWc platelet distribution width 1 2 2 Reagents Only reagents supplied by the manufacturer should be used with the analyzer otherwise accuracy cannot be guaranteed Diluent Isotonic saline solution used to dilute whole blood specimens and to rinse the fluidic system between measuring procedures Lysing reagent Used to create hemolysate for 3 part WBC differential and for total WBC and HGB Cleaner Used to perform cleaning process of the fluidics Rinse Used in cleaning process of the fluidics VET5 Used to dilute whole blood and differentially hemolyse white blood cells to separate eosinophil granulocytes from other WBC by volume It is Suitable to determine EOS 05 BAS and BAS parameters 1 2 3 Technical Operation As Abacus junior vet 5 is a fully automated instrument operating it requires minimal training or technical support Operator interaction is reduced
16. format OB 12 hour format 6 Press to accept the settings 68 8 Printing This chapter covers information on making reports on measured samples 8 1 Printouts When required the following items can be sent to an external printer or to a built in printer by pressing the function key button Database result s table format Database specified patient results with histograms QC result Levey Jennings chart QC result s table format Calibration results Last measured blank result Last measured patient result with histograms Last measured QC result Device information and statistics oelf test result parameters The appropriate printout format can be selected in UTILITIES SETTINGS PRINTER SETTINGS 69 Thermal paper printout 70 Letter printout 5 JUNIOR VET 5 Patient ID 3813 Doctor Dr Smith Lucy Birth Sex _ 12 96 2985 Female Mode Dog Report date 19 86 2007 5 260 2 Test Result Sample ID 33 Test time 12 86 2087 16 19 WBE WBC 18 08 1991 6 m 17 2 83 1984 1 ot 48 8 45 1997 9 2 1 5 5 92 18797 12 8 53 1 9 8 1 gt 1 8 15 1809 1 85 28 2 12 qei 38 i 45 X 7 uu 4 41 8189 58 6 2 87 EOS i 5 3 a 6 RIS 15 x L 3 MR RO GORGE RR 7 29 1 124 5 5 8 5 174 g l 128 e 188 58 01 37 S9 23 9 19 5 os 24 5 348 319 r 348 14 7 AX
17. olide the cassette housing back There are pins on the cassette make sure to drive them in their paths The cassette must click into its place 7 2 7 2 Calibration The Abacus junior vet 5 is pre programmed to monitor DiatroCont 5 control Performing QC determinations regularly verifies continued optimal performance It is recommended to do calibration in the following cases 1 At analyzer installation before beginning the analyses 2 After replacing any component related to the process of dilution measurement 3 When quality control measurements show any systematic error bias or they are outside predefined limits 4 Atregular time intervals determined by the lab itself 5 If you want to use the instrument in Prediluted mode Calibration can be performed in two ways 1 User can enter calibration factors without any calibration measurements using the numerical keypad 2 One two or three fold measurements of control or special calibrations material with known parameters In this case the instrument automatically calculates new factors using the following formula Assigned value x Stored factor New factor Measured value s or average of those CAUTION New calibration will invalidate the previous factors Old values cannot be retrieved but can be reviewed in the VIEW CALIBRATIONS menu Calibration can be initiated by choosing Calibration in the UTILITIES 1 Select the calibrator Calib
18. or 200 240 50 60 2 59 86 F 15 30 C Optimal temperature is 77 F 25 C 12 6 x 10 2 x 14 4 in 320 x 260 x 365 mm 16 Net weight 12 kg 17 2 INSTALLATION 2 1 General information This chapter provides instructions for the installation of the Abacus junior vet 5 hematology analyzer The procedures described below must be followed correctly to ensure proper operation and service Please carefully read and follow all instructions in this User s Manual before attempting to operate Abacus junior vet 5 Abacus junior vet 5 hematology analyzer is a precision instrument and must be handled accordingly Dropping or other improper handling of the instrument will disturb calibrated mechanic and electronic components and or cause other damage Always handle the instrument with care 2 2 Environmental factors Operate Abacus junior vet 5 within the ambient temperature range of 15 30 and relative humidity of 6596 2096 The optimum operating temperature is 25 Avoid using the instrument in areas of extreme high or low temperatures or where it 5 exposed to direct sunlight it is kept at a temperature less than 10 the instrument should be allowed to sit for an hour at the correct room temperature before use Reagents should be stored at a temperature range of 15 30 The instrument should be placed in a well ventilated location The instrument should not be placed near potentially inte
19. that only one item of the group can be selected and this is indicated with a filled circle in front of the selected item Selecting an item of the group will move the filled circle in front of this item emptying the circle of the old selected item 27 3 1 2 Menu structure The following outlines the Abacus junior vet 5 s menu functions D Information Help Displays help for the current screen Analysis Measurement Repeats last sample tested 1 Repeat last sample VETS assigns new sample ID all other patient information is unchanged Performs blank measurement 2 Measure blank Enters automatic calculation for Prediluted mode 1 5 externally diluted sample 1 5 appears in upper left corner of the screen 4 Needle Height Setting D Adjusts sampling depth if xxmm needed 3999 ID Vet5 assigns it automatically Patient ID opecies Doctor Name Birth Sex Displays reference ranges for selected species can be modified 28 Database 3993 1 Go to specified record e 2 Selection Select by date time and ID 2 Select all 3 Deselect all 3 Change sort order Unsorted 2 Sort by time 3 Sort by sample ID 4 Sort by patient ID 4 Manage selected records 5 View external 6 Backup one day Send selected records 2 Delete selected records 3 Backup sele
20. to verify proper operation of essential components of the instrument The Self test should be performed e At installation e After replacing any component After extended time out of use 1 Press the Utilities key and select Diagnostics 2 Select Self test Abacus junior vet 5 then lists and checks subsystems When the test is finished the junior vet 5 displays a summary of the results test results j bate of testing 15 85 2007 ABACUS VET 260802 SW 0 9 FW3 5s 13 86 2897 uccezsftul Press for details 7 5 Settings Selecting item four 4 of the UTILITIES you can access this menu iB Printer settings Customize 3 Date and time 4 Fluid sensors 59 7 5 1 Printer settings The following table lists the available printer selections along with the printer language and specific printer models corresponding to each Table 3 1 Compatible Printers Printer Selection Printer Language Supported Printer Models LPTH245 Built In Special printer language Built in thermal printer Epson ESC P Raster Epson ESC P Raster Epson Stylus series select models only Canon BJC In 9 pin mode Canon BJC Canon BJ and BJC series select models only Canon doi eis HP DeskJet series select models only HP LaserJet series and compatibles LJ1100 etc Epson 24 pin in 9 ESC P2 Epson 24 pin dot matrix series mode Epson 24 pin LQ 580 Epson 44
21. 0 etc FX 980 etc Epson 9 ESC P Epson 9 pin dot matrix series To set up the instrument for your printer go to the Utilities Printer Printer Settings menu Select from the options using the up and down arrow keys within the text fields and fill in the numerical fields using the number keys The general characteristics of the printable area of printer paper are below Paper width Top margin Physical margins R Result Printable area Left margin _ 2 Paper height distance between reports The paper is defined by its size it can be a standard size A4 Letter or any custom sized paper specify the actual size Printers cannot print on the whole surface of the paper The blank area is described by the physical Margins which may vary by printer models The paper area inside the physical margins is called the printable area Top margin and Left margin settings are used for determining the location of printed results on the page If more than one result is to be printed per sheet use the Vertical spacing to specify the 60 On the first page of the Printer Settings sub menu printer type can be selected you can choose between Seiko built in printer or any compatible external printer Initially only the printer driver can be selected and when accepted driver and printout format details become available Choo
22. 40 25724 E 40 Io NER 43 AVC AST TOCA PORT Tm 43 2 9 2 Blank IM eM Ede 43 2 9 2 45 6 DATABASE e 47 7 UTILITIES 49 UN MEME NUUS NINE Ts 49 db 49 1452 Weekly 51 INE Lo S CES Dice QUES NT 57 E XN iiio 59 M 59 60 SEE GI IZ 64 o _4 _ ___ ____ _ _ __6_6_______ _ _ 66 fA ___________ ____ 68 ME RUIT 69 72 I0 Warning labels on E EIER EI EISE eS 73 1 INTRODUCTION 1 1 Intended Use Abacus junior vet 5 hematology analyzer is a fully automated cell counter designed for vitro diagnostic use The compact instruments were developed for small clinics point of cares and vet offices 1 2 The Instrument Abacus junior vet 5 is a fully automated bench top hematology cell counter It implements the so called Coulter method for counting cells passing through small aperture and measures the hemoglobin content of red blood cells The analyzer features a graphical LCD display mod
23. D display a 29 button keypad and USB and serial computer interfaces 1 4 1 Function of the Fluidics For the Schematics of the fluidics system see Section 9 Sample aspiration and dilution a Stages of the blood testing process sampling needle mixed with VET5 reagent and diluent for the 5 part 25 of anti coagulated EDTA whole blood sample is aspirated through the differentiation After the first counting and washing process another 25 ul of anti coagulated EDTA whole blood sample is aspirated again and mixed with 4 ml of diluent and stored in the chamber mix dilution 25 ul of the mix dilution is aspirated into and stored in the needle during WBC 11 measurement and the hemoglobin analysis Lysing reagent is added to the mix dilution held in the chamber for WBC differential analysis This amount of lysing reagent is species dependent and may be changed by the operator within Limits menu After WBC counting and a washing process 5 ml of diluent is added to the second dilution using the 25 ul of mix dilution stored in the needle This portion is analyzed for RBC count PLT count and their parameters Table 1 Dilution rates used within acus junior vet 5 e DIFF dilution 1 160 e Mix dilution 1 160 e RBC dilution 1 32 000 e WBC dilution 1 196 depends on lyse amount Measurement times DIFF measurement 8 seconds WBC count 8 seconds HGB measurement 3 seconds RBC PLT count 8 second
24. E 1 68 1 X 18 8 HGB 133 RDWc 28 1 i MORE 36 531 17 8 5 99 _ 45 388 7 34 ED 3 91 PLT 15T FDWc 31 8 APY TALIPCT 1 11 Weg En Flags Hane Flags Hone 13993 __ 19939 _ UTILITIES 6 DATABASE Patient results are stored in the memory in chronological order and can be retrieved at any time Memorizing capacity is 2 000 measurements including the complete parameter list histograms flags sample data and date time of measurements If memory is full latest actual record will overwrite oldest record To access the Database table press the Database key the front panel The first screen that appears shows the most recent saved tests DATABASE 12 86 2007 Pressing the lt key accesses the 12 86 2887 remaining parameter results The 12 86 2887 T or 4 key scrolls between the results 12 06 2087 individually 12 06 2007 To display the WBC RBC PLT and VETS histograms press the l 2 soft key To print an individual result highlight the result and press Print Database local menu iB 50 to specified record DATABASE LOCAL MENU e Selection 3 Change sort order 4 Manage selected records From the database table screen enter the 5 View external Database local menu by pressing the Menu Backup day key The menu contains the following items 47 UTILITIES
25. UM AJV5 01 1060 Revision history Edited by Sections affected Approved by L S le C Magyar C Magyar I Losonczi New pump new power suppl C Magyar cover page p7 Front panel p9 10 AXIOHM printer added 011 power supply image changed M Switzer Update Address 5 part and 3 part Species Rev Date 0 10 2007 06 27 2007 07 05 2009 03 23 2009 10 13 2012 02 24 2012 12 3 Re p SS __ p M M HEN _________ __ p HEN p E p RM KENNEN Signature Abacus junior vets Hematology Analyzer Users Manual 1 06 Release DIATRON MI PLC H 1097 Budapest Tablas utca 39 HUNGARY Tel 36 1 43 9800 Fax 36 1 436 9809 www diatron com support diatron com pistron Contents Ll INTRODUC FION 550 5033509 PLE IEEE REI YS OH aaia rre Ea 4 4 1227 MMS STUN 4 5 BROAN asses _ _ ___ __ _____ 6 L29 Technical OD 6 1 2 4 Calibration and Quality 6 Lo mon a NETT 7 bo yZ RETI 11 LO o PUBeHoB OL MS A E E S 11 LA 0 01 5 NORTE 12 __ ___________4 __ __ 12 M 13 Lo MEOS 15 IEEE em 15 I E E __ 16 2
26. al pre dilution of the sample using cAbacus junior vet 5 reagent diluent or an isotonic saline solution Dilute the sample to 1 5 ratio 1 part sample to 5 part diluent Mix it well To perform the analysis 1 Press the Measurement Analysis key 2 then press the soft key 14 06 2007 15 31 FLT HPV 2 Select Prediluted Mode Measure local menu 1 Repeat last sample 2 Measure blank ORS Prediluted mode 4 Heedle Height Setting mm FCT 14 06 2007 15 54 HGE FLT HPV Flags Hane Fredil 14 06 2007 15 32 UETE IFFobe 14 06 2007 16 34 UETE FFobe FLT Lsze Flags Hone 45 UTILITIES 4 f you are processing a new sample Press the soft key and enter the patient information then press EVE sample information j Date 14 06 2007 16 34 i BILE Sex 5 If you are processing a sample that has already been tested as non prediluted select Repeat Last Sample to avoid having to re enter patient information Measure local menu 1 kepeat last sample 2 Measure blank Os Frediluted mode 4 Heedle Height Setting Bs B mm 6 Press Start button to begin the analysis cAbacus junior vet 5 will automatically calculate the results with the 1 5 pre dilution factor Horse 1 11 85 2857 15 28 11 66 2087 16 28 uUETE Fr aba pta eee RBC dla
27. aste Container 20 L Abacus junior vet 5 External power supply and Power cable Abacus junior vet 5 Tube adapters Abacus junior vet 5 Thermal roll paper Reagent Tubing Kit Cleaning Tube Kit 15 1 7 5 Specifications Chambers Chambers Reagent system Aperture diameter Throughput Characteristics Accuracy Reproducibility CV Sampling method Sample types Clog prevention Cleaning procedure Quality control Calibration Multi user feature advanced feature User interface Languages available Data capacity Host computer interface Data back up method Software upgrade method Printer drivers Built in printer Display Keypad External keyboard Power requirement Power supply unit Operating temperature Dimensions W x D x H 25 ul of whole blood in 3 part mode 50 ul of whole blood in 5 part mode 50 ul of whole blood in pre diluted 3 part mode 1 unified chamber for diluting whole blood and counting Isotonic Diluent Lyse VET5 Lyse Cleaner and Rinse 80 um 22 28 tests hour in 3 part mode 15 20 tests hour in 5 part mode Carry over sample to Test range sample Correlation coefficient R 4 0 20 0 10 ul 4 0 15 0 109 ul 25 0 50 0 6 16 1 200 900 107 ul 15 50 25 80 0 5 2 0 10 ul Open tube system with automatic sample rotor Dog Horse Cow Cat Calibrator QC material 5 part mode Ferret Goat Guinea Pig Mouse Pig Primate Rabbit Rat Sheep 3 part mode
28. central plastic roller of old paper roll unwind new paper roll so that the starting edge is coming from down under towards you gently drop the new roll into the holder of the printer and hold the starting edge with your hand and make sure it comes out on the front of the printer close the lid making sure that the paper is captured between the lid and the front of the printer 10 The analyzer works with an external power supply The next figure shows the power supply unit generating 12VDC The power supply module has a so called auto range input allowing operation on 230V or 115V power system The power supply complies CE and UL safety certifications The input socket is a standard power cable connection the output is a special lockable socket as shown in the picture Only the provided power supply shall be used with the instrument Protek Electronics Corp Model ID PUP80 12 N8 or GLOBTEK INC Model ID GT 81081 6012 T3 1 4 Parts of the Analyzer Abacus junior vet 5 hematology analyzer is composed of three main units Fluidic System Data Processing System Control Panel Performs sampling diluting mixing and lysing and Lyse2 lysing functions Generates the regulated vacuum used for moving cells through the aperture during the counting process Counts measures calculates blood parameters generates and stores numerical results and histograms Features an LC
29. cted records Selects one record by time date Sample ID and or Patient ID more criteria narrows search Selects one or more records by range of times dates sample IDs Selects all records Deselects all records List records in the order entered most recent to oldest Lists records by measurement time oldest to most recent Lists records by sample ID highest to lowest Lists records by patient ID highest to lowest Sends selected records to a computer through a serial connection Deletes selected records from the VET5 database Saves selected records to USB drive Reads stored records from USB drive Backs up all results from specified date to USB drive insert USB drive Toggle between graph and table display of selected results Opens dialog for viewing modifying patient information sample ID and patient type cannot be changed Sample ID Patient ID Species Doctor Name Birth Sex 20 1 Maintenance 1 Cleaning 1 Auto self cleaning 2 Hard cleaning 2 Priming 1 Prime diluent 2 Prime lyse 3 Prime rinse 4 Prime cleaner J 5 Prime lyse VETS 6 Prime all 3 Drain chamber 4 Reagent status 5 Bleaching 6 Software upgrade 2 Calibration 1 Calibrate 2 View Calibration History Calibration setting 3 Quality control 1 Set QC reference ranges 2 Run QC 3 View
30. current flowing between the external and internal electrodes causes some change in the impedance of the conductive blood cell suspension These changes are recorded as increases in the voltage between the electrodes The number of pulses is proportional to the number of particles The intensity of each pulse is proportional to the volume of that particle The volume distribution of the cells are displayed on diagrams WBC EOS RBC and PLT histograms 4 2 Principle of HGB Measurement The lysed 1 196 sample dilution can be measured by a cyanmethemoglobin method The reagent lyses the red blood cells which release hemoglobin Hemoglobin iron is converted from the ferrous Fe to the ferric Fe state to form methemoglobin which combines with potassium cyanide KCN to produce the stable cyanmethemoglobin or hemoglobincyanide Subsequently the concentration 15 measured photometrically Note The above mentioned measuring method is used to determine the HGB concentration The HGB concentration can be measured using cyan free lysing reagents as well In this case the effect is the same but the used lyse is environmental friendly reagent 4 3 Parameters Abacus junior vet 5 measures and calculates 22 parameters listed below For each parameter we list the name abbreviation and measurement unit in the first column Short description for each parameter is in the second column White Blood Cells Number of leu
31. d with water to clean this area You can see the washing head indicated in the following figure Washing head Measuring chamber Measuring aperture Figure13 Parts of measuring block 1 Exit the Measurement menu Open the side door after the needle has stopped moving 2 Gently rub the lower surface of the washing head to remove the salt build up 3 Close the side door Peristaltic pump maintenance The pump installed in the instrument is maintenance free However would you experience leakage from the pump or vacuum error you may replace the tube used Remove the tubes from the pump by opening the screw connectors Push in the two sides of the pump cassette Pull the cassette off the pump Pushing aside the two tubes bend the tube out of the pump Now you have the tube as figure 1 shows Using a standard screwdriver ease the tube on the plastic connector and pull the tube off Repeat the same procedure on the other end of the tube 53 Now you can pull the plastic holders off the tube Retain all parts except the old tube Using the new pump tube slide the two grey plastic parts onto the new tube Make sure they are aligned as shown on figure 1 Insert the 2 white plastic connectors into the ends of the tube The new set must look like the tube on figure 1 Put the tube back into the pump mechanics and drive the grey plastic parts into their seats as figure 2 indicates view from the top of the pump
32. deletes previous QC results It is strongly recommended to print results prior to changes 1 In the Quality Control menu select Set QC reference ranges control reference ranges 2 Er QC 3 View table of measures 4 view 0 diagram 5 Set Qc Level Actual BC Levels 2 QC Reference values 57 3 12 zT ATA ml T 3H HGH 10737 4 46 8 28 1B 12 l 3 Entert the value ranges for each test parameter as indicated on the paperwork accompanying the QC lot number you are using e Use the keypad to change displayed values e to disable QC of a parameter set it to 0 0 e use the PGDN and PGUP to view additional parameters 4 Press to accept the data then press to confirme 5 Select Run QC to start the analysis Press the Start button QC GU T1711 11 25 T lT s Mere LY 7 49 110 3 62 HEX 86 FD PLT 258 8 11 NPY a Flags Hone Press to accept the results and save them in the QC database Viewing the Accepted QC Database 1 Press the Utilities key then select Quality Control 2 Select View table of QC measures B5 2 BET 08 06 2007 00 06 2007 38 86 2007 BG Bb 2807 aC 1 41 88 06 2807 58 7 4 Diagnostics Devices il Device information ABACUS VET 5 2 Statistics zb 3 test ian f 2 55 13 06 2007 Self Test The Self test is a procedure
33. e draws sample from the tube The aspirating needle is retracted while its outer surface is automatically rinsed with diluent This insures a low carry over between samples After a few seconds the rotor turns out Now you can remove the sample tube from the adapter of the sample rotor 38 5 2 Sample analysis 5 2 1 Sample preparation Use K EDTA anti coagulated fresh whole blood as sample Prior to sampling mix the sample gently by inverting it 11 times Do not shake as this could damage the blood cells 5 2 2 Modifying lyse quantity The default lyse quantity can be adjusted by pressing on the MEASURE screen Another option is to modify the lyse quantity by 0 1 ml or 0 2 ml is available during analysis Press f to increase the lyse quantity 0 1 0 2 ml if the separation between lysed RBCs and WBC populations is poorly differentiated resulting in increased WBC and LYM counts Press to decrease the lyse amount 0 1 0 2 ml if the WBC histogram seems to be shrunk to the left i e the different WBC populations are overlapped This can inhibit proper separation of WBC populations MES ml method ml method 3 598 ml 8 I 109 1 The two important parameters influencing lysing are lysing time and lyse quantity You cannot change the lysing time as it is adjusted to the lysing reagent supplied by Diatron Above you could read that the lyse quantity can be adjusted either at patient l
34. e given parameter will be highlighted as well You can customize ranges for all kind of patients by setting the corresponding lower and upper ranges If you set 0 for a range limit it will be not verified Horse 5 aBC 7 47 BL LYM S2 S HGB 12B5 KDWc HEU 45 5 5 1 3 FLT imT PDWc Hz HPV Fat PCT Flags Hane 19999 42 5 3 Measure 5 3 1 Measure local menu 5 3 2 Blank Measurement Blank measurement is used for checking the cleanliness of the system and the reagenis Blank measurement must be performed e Once daily before sample analysis this is done automatically before the first analysis in MEASURE function e After any reagent change activated manually from the MEASURE MEASURE BLANK menu e After the replacement of any hardware component that is closely related to the measuring process aspiration dilution counting rinsing Press the Measure key and press the button on the screen and select Measure blank Measure local menu 1 Repeat last sample EA Measure blank L13 Prediluted mode 4 Heedle Height Setting BH mm 13 H5 2HH 16 59 13 H5 2HHT 16 59 AR VETS VETERES HGB A EDW MEY MCHC FLT 1 PFDWc HPV a T Flags Hone Flags Hone 113333 I 13333 When the Blank measurement is OK press to accept the result The Abacus junior vet 5 is then ready for analysis The instrument then dis
35. erence ranges To print numeric values select the displayed range for example 4 51 5 64 None prints no range values Graphical prints a graph showing where the results falls in range L H Graph prints the low and high limits of the range followed by a graph showing where the result falls in the range Graph L H prints a graph showing where the result falls in the range fol lowed by the low and high limits of the range L Graph H prints the low limit of the range a qraph showing where the result falls in the range and the high limit of the range Select Yes to include in the printout measured values that fell outside the reference range as well as any errors during analysis Print warning flags Select Yes to include in the printout all flags that appeared in the results Clogging report Select Yes to include information on aperture clogging on all printouts Select Yes to include theVET5 s serial number on all printouts result Press the Soft key to accept the settings 63 7 5 2 Customize Use the Customize menu to set the language and date format used by Abacus junior vet 5 along with the screen saver delay and the date range used for printing combined results from the Abacus junior vet 5 1 Press the Utilities key 2 Select Settings Utilities 1 Hanienance 2 Calibration 3 coniro 4 DLacnoaztlcs 3 Select Customize 1 Frinter settings EB Customize 3 Date and t
36. ime 4 Fluid sensors General Settings 2 Units 3 Laboratory 4 User modes English Offline Serial Protocol Version Z A Serial s Decimal Dot lang del vas Links Offline Date format of VetScan Do Hn pv 64 Table 3 4 describes the available settings Table 3 4 General Settings screen saver wait time 0 to 30 minutes 0 screen saver off Language English International customers please contact your distributor for information about available languages PC Link Offline USB Baud rate 150 to 115200 Protocol used by the VETS to communicate with a connected computer the VET5 is not connected to a computer choose Offline If the VET5 is connected to a computer using a USB cable choose USB Serial Protocol Version 1 0 1 7 2 20 2 23 3 0 or 3 1 Protocol versions used by practice management software to interface with the serial s Decimal Dot Dot period slash comma or lang def as defined by language Link Offline USB 85232 Protocol used by the Veti to communicate with a connected VetScan Classic or VS2 for combining results If the Vet5 is not connected to VetScan Classic or V S2 choose Offline f the Vet5 is connected to a VetScan VS2 through a USB cable choose USB If the Vet5 is connected to VetScan Classic through a serial cable choose 5232 Date format of VetScan When printing combined Vet5 and VetScan results the date format used by the VetScan MM DD YY or DD MM Y Y
37. imits or right before measurement 39 5 2 3 Sample information The software allows the user to enter information for each sample that has been or will be measured an external PC keyboard PS2 is used it must be connected to the instrument before turning the instrument on Two options exist for sample information entry e immediately before analysis e inthe Database menu To enter sample information prior to sample analysis press the Measurement Anlysis key and press button on the screen The following screen appears the keypad OK key and and gt Samplei f keys to move through the settings Date 14 86 2887 11 23 Use the Sand V keys to change the settings and the keypad or external keyboard to enter text or numbers Press the to accept data cancel with button Begin analysis by pressing the Start button WARNING Do not reach inside the instrument as the needle can injure you 5 2 4 Results When analysis is complete the following screen is displayed including all measured and calculated parameters as well as the WBC RBC PLT and VET5 histograms Results and histograms will be stored automatically in the memory Horse 1 11 06 2007 15 28 11 86 2887 16 28 8 73 RBC 815 ri METER 02 i 5E LY 18 8 HGB 139 RDWc 28 1 i B 5T MOM T T HCT 35 53 MCH 17 9 5 99 BB 5 MCV 45 MCHC 388 M4 ELT 9 15T PDWc 31 6 HA HB HPV T L FCT 1
38. k is high or no LE RBC blank Same action as in case of warning flag p RBC PLT clogging The same action as in case of the C warning flag Table 5 Summary of warning flags related to EOS problems Result a Flags Meaning ________ ________ Similar to the WBC B Run a cleaning EOS Blank high cycle and rerun blank The Lyse2 measurement has encountered a clogging in the aperture Run a cleaning Y Clogging cycle and repeat sample measurement Possible lysing problem Check if there is enough reagent in the container Prime the Noise in the EOS Channel Lyse2 reagent 41 Warning flags can be grouped according to measurement conditions and according to the problems relating to the blood sample Measurement conditions when the flags are related to clogging c y C probably hemolysing problems E b B p z and pressure problems Fatal pressure error In this case we suggest repeating the measurement The asterisk flag near a parameter shows some doubt suspected during the evaluation of that parameter The reasons can be a high PLT blank PLT value will be marked a case of indefinite discriminator setting default location is used for some reasons related parameters will be marked etc Another flagging method is evaluation against the normal ranges some of the parameters is out of range it gets a flag if under the range or gets if over the range And th
39. kocytes cells l cells ul WBC x cells l cells ul cells l cells ul RBC RBCea x cells l cells ul Hemoglobin concentration HGB Measured photometrically at 540 nm in each cycle blank measurement is performed on diluent g dl g l mmol l HGB HGBmeasured fl histogram Hematocrit HCT Calculated from the RBC and MCV values HCT percentage RBC x MCV x 100 percentage absolute absolute RBC x MCV Mean Corpuscular Hemoglobin Average hemoglobin content of erythrocytes calculated from pg fmol RBC and HGB values MCH HGB RBC Mean Corpuscular Hemoglobin Calculated from the HGB and HCT values Concentration MCHC MCHC HGB Unit of measurement is displayed according to the one chosen for HGB result g dl or mmol l Red Cell Distribution Width RDW SD fl The distribution width of the erythrocyte or platelet population Platelet Distribution Width PDW SD fl derived from the histogram at 20 of peak n 100 Red cell Distribution Width RDW CV absolute Platelet Distribution Width PDW CV absolute xDW SD x P2 P1 fl xDW CV x 0 56 x P2 P1 P2 P1 by the factor of 0 56 CV is corrected to the 6096 cut Platelet PLT Number of thrombocytes platelets cells l cells ul PLT PLTca x cells l cells ul fl histogram Thrombocrit PCT Calculated from the PLT and
40. ntenance Jobs From Maintenance submenu the user can initiate maintenance procedures such as cleaning priming draining chamber reagent status or bleaching MAINTENANCE 1 Cleaning 4 Select the required submenu 2 Friming 3 Drain chamber 4 Reagent status 5 Bleaching Software Upgrade 7 1 1 1 Cleaning Item 1 in the above menu brings up cleaning functions Item 1 starts a washing cycle using the 1 Automatic self cleaning system cleaner reagent This action is 2 Hard Cleaning recommended if clogging problems experienced C or Q error flag Item 2 initiates a process that uses a light solution of hypochlorite NaOCL washes the entire system with it The instrument will ask for the cleaning solution in a sampling tube 49 UTILITIES 7 1 1 2 Priming During the priming cycle the fluidic system is RE rinsed with a large amount of diluent It differs from the process in start up procedure as 3 Frime rinse m i in the latter case a simple filling up of the Prime cleaner E i fluidics is performed If fluid sensors are on then the analyzer makes these procedures automatically otherwise the User must initiate them activating the appropriate item within this submenu 5 Frime lyse VETS Frime all 7 1 1 3 Draining chambers Draining of chambers should be run before removal or replacement of parts related to the measuring chambers or apertures
41. o an AC outlet Host Computer The instrument has a built in serial port that allows connection to a host computer Results including histograms may be exported Serial USB I O settings can be found in Settings For installation instructions please contact Service Power supply Connect the power supply to the instrument Attach power cord outlet to the external power supply of A4bacus junior vet 5 and plug the other end into a properly grounded AC outlet Do not switch on the instrument before connecting the external power supply to the instrument and to the AC outlet as well as before connecting an external printer or a keyboard to the instrument 21 7 Reagent Containers Place the reagent containers near the instrument to an accessible location Do not place the containers to a higher position than that of Abacus junior vet 5 because would a tube come off its connector the fluids spoil out Use the supplied connecting tubes and special bottle caps Be sure that the color on each tube cap and connector in the back of the instrument match You can for example place the reagent containers below the desk Abacus junior vet 5 is installed on as the instrument has sufficient power to draw the liquids from a lower location All containers should be left open do not block the small air vent hole on the special container caps in order to provide free airflow For connections see Figure 5 Diluent
42. omponent A into the waste container when it is empty then connect it to the analyzer When the waste container becomes full replace it Wait 1 2 hours for oxidation to complete Put 5 of component B into the waste container Wait 2 3 hours 2 Neutralization of biohazard effect Independently of the fact that the waste contains cyanide or not you should make this step Put 2 hypochlorite solution into the waste Close the cap shake the container and wait 1 hour Dispose of waste by spilling it into the drain system 7 1 2 Weekly maintenance Weekly maintenance should be performed before turning on the power switch e How to open the side doors On the left side and the rear side of both instruments there is a side door which gives access to the fluidic system and the mechanical parts easily Figure 11 and 12 Other parts of the analyzer electronic parts etc can be accessed by opening the front cover and the rear cover 51 UTILITIES Needle moving mechanics Washing head Micro dilutor Chamber and Amplifier aperture assembly Reagent Sample rotor inlets Valve block Figure 11 12 Reagent sensors Valve block Reagent Main dilutor connectors Peristaltic pump 52 UTILITIES Cleaning the washing head The washing head cleans the outer surface of the aspirating tip with saline diluent Any salt build up on the lower surface may cause malfunction during operation Use and a soft cloth dampene
43. onal regulations the system is operated according to instructions contained herein 20 2 3 Unpacking and installation Carefully remove the Ahbacus junior vet 5 hematology analyzer from the shipping carton Inspect the instrument for any visible signs of damage incurred during shipping Would you find any damage file a claim with the carrier or your distributor immediately Check the accessories received against the packing list Contact Service if anything is missing CAUTION Prior to initial operation allow the instrument to reach room temperature approx 2 hours temperature changes in an operational unit can lead to water condensation which may damage electronic parts Place the instrument on a firm work surface in the designated work area near an appropriate AC electrical outlet The connection MUST be grounded NOTE Before making connections Make sure that all power is in OFF setting before connections printer external keyboard are made Carefully read all literature accompanying the instrument and its accessories Pay particular attention to the operating procedures for the external printer 4 Keyboard and external printer Attach the keyboard cable to the round KEYBOARD port on the back of the instrument Attach both ends of the printer cable to the appropriate ports on the printer and Abacus junior vet 5 Attach the AC adapter to the printer if required and plug it int
44. over of analyzer or the furniture it has to be wiped off immediately In case of skin contact the liquid has to be rinsed off with plenty of water Waste generated by the unit is biohazard material Handling and disposal must happen according to regulations regarding reagent systems WARNING Waste contains poisonous substances because of chemical content and human origin substances causing biohazard These substances are representing potential danger to environment For this reason safe handling of the waste liquid is very important 2 2 5 Maintenance The user should check the following components weekly bottom of washing head for salt build up should be wiped off with a damp cloth tubing system by opening the side and back doors and look for any liquid leakage If any leakage is observed authorized technician should be contacted Caution The following parts must NOT be opened or serviced by the user electrical supply electronic boards 2 2 6 Cleaning The instrument and its power supply should be cleaned on the outside only using a damp cloth with a soft detergent DO NOT let liquids get inside the units 2 2 General points The manufacturer guarantees work safety reliability and general characteristics under the following conditions only Services and repairs are performed by an authorized technician the electrical system of the laboratory follows national and or internati
45. plays a sample measurement screen as shown and is now ready to perform an analysis 43 14 06 2007 16 31 14 06 2007 16 32 VETS robe HGE HCT MCH MCHC REC PLT Lsze PLT HP Flags Hone Flags Hone 13333 113333 i EXE There are 3 regions for blank value handling 1 Optimal all results are within acceptable ranges 2 Blank is high flag is displayed at relevant results 3 Blank exceeds acceptability no results displayed 1 No flag at parameter 2 flag at result 3 E error flag at result EOS 0 0 1 x10 cells ul gt 0 2 x10 cells ul Table 6 Blank measurement ranges Accepted blank values are essential for proper calibration and quality control measurement For this reason no calibration or QC measurement can be performed without accepted blank values Quality control measurement and calibration can be performed only if all blank values are in the first region receiving no flags or errors If analysis errors occur or the blank measurement is too high an E error flag appears along with the affected parameter and is displayed instead of results In this situation perform a cleaning UNSUCCESSFUL BLANK MEASURE UNSUCCESSFUL BLANK MEASURE VETS Frobe EER eE HGE A RDWc i HCT VE WEC MCV REC FLT Lase Zu Foc xm PCT Flags chply Flags chply 44 5 3 3 Using Pre diluted Mode Perform an extern
46. rate Control 2 View Calibration History 2 bag 3 Calibration settings 3 Cat 4 Horse 55 2 Enter the assay values for each parameter from the calibrator package insert Calibration Calibration 3 When all parameters are set press the soft key The display shows Calib 1 1 at top left 16 86 2087 10 53 1 1 18 06 2007 10 53 WEC VETS Frobe HGB FOWE MCHC REC FLT Lsze PLT HP Flags Hone Flags None 8 0 0 0 120 90 800 2 15 90 300 10 30 0 Table 7 Calibration ranges 1 90 10 30 5 5 30 1 0 9 When analysis is complete and the display shows the results press i 56 171 1 2 BAL 3 89 WBC 8 46 izT RDWc z1 9 LYM 3 41 LY 30 86 MCH 32 8 MOHM 0 76 0 9 413 MEU 4 29 HEX PLT 247 34 61 05 5 74 0 ion iz T PCT 31 BAS 8 131685 ae Flags Hone Flags Hone Calibration 7 3 Quality Control Procedure By analyzing control materials day to day reproducibility can be monitored In this submenu both target values and acceptable ranges for each parameter can be specified for different QC levels NOTE Target values of the control material should be set only once at the beginning of the QC measurements Resetting parameters deletes previous QC results of the active level CAUTION Any change in the QC material setting
47. rfering devices capable of emitting radio frequencies e g radio or television receiver radars centrifuge X ray devices fans etc Operation at an altitude over 3000 meters 9000 ft is not recommended Instrument is designed to be safe for transient voltages to INSTALLATION CATEGORY and POLLUTION DEGREE 2 Environmental and electrical requirements have been provided to insure the accuracy and precision of the instrument and maintain a high level of operational safety for lab personnel 2 2 1 Electrical requirements cAbacus junior vet 5 comes with a power cord appropriate for your power system Proper use of the appropriate power cord assures adequate grounding of the system WARNING Failure to proper ground the junior vet 5 bypasses important safety features and may result in electrical hazard 18 2 2 2 Space requirements It is important to install the instrument in a suitable location A poor location can adversely affect its performance Consider the following space requirements Select a location near a power source and close to a suitable drain Place the unit on a clean and level surface Leave at least 0 5 m space on both sides and above the instrument to access pneumatics and optional built in printer minimum of 0 2 m must be maintained between the rear panel and the wall to allow for heat dissipation and tube clearance Install the reagents in a suitable place that will make your work easy
48. rinter Settings Setting Description Printer used with the VETS see Table 3 1 Printout format Overall format used for printouts Available selections depend on the selected Printer See page 3 6 examples Table format Printed width of tables included in the printouts Printer cable Interface used for an external printer LPT parallel or USB Paper type used in the selected Printer Units Default units of measure paper size and print margins for the selected Paper size Paper These can be customized if you re using a non standard paper Left margin Left and top margins control the position of printed results on the page Top margin Table 3 2 Printer Settings setting Description _______________ Printing mode Overall printout size Mini Small Normal Enhanced or Large width Wide or Narrow and print speed Fast The available modes depend on the selected Printer Physical margin Margin correction for printers that have smaller printable areas than usual Use Normal unless the right edge of the printed text is missing or appears in the next lina One result per Select Yes to print each analysis on a separate Hallpaper Select Yes if using the built in printer Vertical spacing The vertical distance between printed results when multiple reports are printed on each page Select Yes to print all results automatically after each analysis Limits format The format for printing the ref
49. s 1 4 2 Control Panels START button Pressing and releasing the START button triggers an analysis cycle Status indicator A three color LED is located near the START button 15 actual color indicates the status of the analyzer LED color Analyzer status e Green The analyzer is ready to work Analysis can be initiated e Red blinking Blood sample can be removed when the LED blinks red 3 times and the instrument beeps 3 times e Yellow The analyzer is performing a maintenance process e Yellow blinking The instrument is in stand by and display light is off 1 4 8 Display The display is a 240 x 128 dots high contrast backlit graphic LCD Liquid Crystal Display module The analyzer is currently performing an analysis No new measurement can be started 12 1 4 4 Keyboard The foil keypad is composed of the following shown in Section 1 3 Figure 1 Numeric keys for entering numerical data and selecting menu items Function keys with specific functions These functions are menu dependent and are indicated by icons appearing above the keys Hardware function keys short cut keys for easier navigation between menus Cursor control keys T and for moving between database items lt and for moving between parameter columns or menu levels START key for initiating an analysis cycle OK key for confirming data Del key for deleting characters Help key for HELP function 13 Function keys Below is a list of po
50. s liter Table 3 Linearity ranges of Pre dilution mode 35 5 ROUTINE UTILIZATION and MEASURE 5 1 Sample handling oince some time will usually elapse between collection of samples and counting it is necessary to preserve the sample with an anti coagulant to prevent large groups of cells forming into clots or lumps of cell matter that will clog the cell counter Choice of anti coagulant is very important as some anticoagulants will affect the shape and size of blood cells In general EDTA preferably potassium based is the only anti coagulant recommended for use with electronic blood counters Care must be taken when using homemade containers pre dosed with EDTA If the container is not filled with enough blood the ratio of EDTA to blood may reach a level which results in osmotic transfer from the RBCs which shrinks them The ratio of EDTA to blood should not exceed 3 mg ml Generally we suggest using pre manufactured sample tubes containing the necessary amount of EDTA Also when taking blood please make sure that requirements attached to sample tubes are met Important Sample tubes must be filled to at least 7 8 mm height with blood otherwise correct sampling is not guaranteed There is another possibility that can help the user in correct sampling by using the needle setting function This is available in Measurement Local menu and controls sampling height of the needle inside the sample tube So if you have a sample
51. se printer type seiko LPTH245 Built in select the Printer matching your printer hardware Pressing the key will bring up printout details dialogs Printout format Narrow full with histograms or Narrow text only without histograms Table format Normal or Narrow Printer cable COM Paper RollPaper Units inch or cm Printer settings 3 Paper size 2 24 x 50 00 RallPaper Left margin 0 00 cm Paper size x 12780 Lett margin I ri Top margin i p Printing mode Harmal Top margin 0 00 O O Printing mode Normal 61 Physical margin normal One result per page No Hollpaper Yes Vertical spacing 1 27 Autoprint Yes Limits format L Graph H Physical margin One result per Vertical spacing Autoprint Ho Limits format L Graph H Print flags Yes Print warning flags Yes Clogging report No Serial number on result Yes Print tlags Print warning tlags B For an external printer the following default settings are recommended Printout format Full with histograms or Wide text only results are printed without histograms Paper select as appropriate for your printer Note Never select Rollpaper for an HP LaserJet Left and top margins 1 00 Rollpaper No especially for HP LaserJets Configure any other settings as needed See Table 3 2 for a complete list 62 Table 3 2 P
52. ssible icons and functions assigned to the so called soft keys function keys Function Action triggered Exit from actual menu or action Leave data entry menu without saving any changes made to it Cancel HE DONN erna ____ Below list the icons and functions assigned to so called hardware function buttons Function Action triggered key Measuring process at once x 0 Je Msg M Utilities menu Printing function Gp oo 14 1 5 Control Material Abacus junior vet 5 allows continuous monitoring with DiatroCont5 control control blood This should be matched to the types of samples usually run on the instrument Specification for this material assay values and allowed tolerances along with expiry date is always enclosed with the approved control material 1 6 Accessories Below is a list of accessories shipped with your eAbacus junior vet 5 instrument This list can also be referred as the Abacus junior vet 5 pack Abacus junior vet 5 Hematology Analyzer Abacus junior vet 5 User s Manual this booklet Abacus junior vet 5 Reagent Tubes with colored connector caps Diluent tube green Lyse tube yellow VETS tube orange yellow Clean tube blue Waste tube red Rinse tube white Abacus junior vet 5 Cleaning Tube Kit Abacus junior vet 5 Caps for reagent containers matching reagent tube connector cap colors Abacus junior vet 5 W
53. table of QC measures 4 View QC diagram 5 Select QC type 1 QC Level from1 6 continued 1 Control 2 Dog Horse Cow Cat Runs cleaning cycle to remove build up from aperture Primes reagent lines use after replacing reagent Primes diluent line green connector Primes lyse line yellow connector Primes rinse line white connector Primes cleaner line blue connector Primes lyse VET5 line orange connector Primes all reagent lines Drains aperture chamber use before moving 5 Displays reagent installation date usage and remaining life Bleaches internal lines perform with each change of reagent pack Prompts for software upgrade disk and restarts system Performs calibration Calibrates using DiatroCont 5 calibrator material Displays previous calibrations Updates QC ranges for current QC lot number Runs QC analysis Displays previous QC measures Displays previous QC measures as Levey Jennings curve 30 4 Diagnostics 5 Settings 6 Service Device information 2 Statistics 3 Self test Printer settings 2 Customize General Settings 2 Units 3 Laboratory 4 User modes Single user mode 2 Multi user mode 3 Date and time 4 Fluid sensors Diluent amp Cleaner 2 Lyse 3 Lyse VETS 4 Rinse 5 Calibrate sensors Sho
54. the optimal working temperature In some cases a priming cycle is necessary prior to sample introduction The instrument will perform the cycle automatically if the fluid sensors are on and additional liquid in the tubing system is required A priming cycle should be run e atinstallation e in case of extended time out of use e after replacement of any component related to the Fluidic oystem e reagents are replaced with the instrument turned on 2 3 2 Turning the Instrument OFF ep The instrument should never be switched off by simply flipping the power button on the rear panel Doing so may result in erroneous operation during later use It can be SO because the instrument uses different kinds of solutions one of which is the so called diluent This liquid is an isotonic saline solution containing salt Would it not be washed out of special units of the instrument or would chambers not be filled with this solution may lead to dust condensation or salt build up Therefore always follow the instructions below when switching the instrument off Press EXIT key Mon the front panel The following screen is displayed EXIT SHUT DOWN 1 iB Shut down 2 Freparing for shipment From the Exit menu select Shut down When prompted press to confirm Abacus junior vet 5 will display a message and give a tone indicating that it is safe to shut it off Turn Abacus junior vet 5 off using the power switch on the rear panel
55. tinguisher if necessary 25 3 MENU SYSTEM 3 1 General Information This chapter contains information about the structure and usage of the software implemented menu structure This integrated software controls instrument operations including calculation and evaluation of measured data displaying results and information screens storage and recalling of data 3 1 1 Navigating in the Menu System The instrument uses a menu system to initiate actions and allow access to settings There are four possible ways to navigate between menus and menu items The most important issue is that there are 6 function keys above the LCD screen You use these buttons to navigate between main functions of the instrument eee These keys are short cut keys by pressing any of them you can directly access main functions whichever submenu you are in If you use an external keyboard you can access these functions with keys F8 through F12 described in Menu Structure as well tabs showing the active tab accessible tab main functions By pressing the short cut keys you can iB last sample activate main functions Information 2 Measure blank Measure Database Utilities Printing Exit 03 Prediluted mode of the instrument Below the short cut keys 4 Needle Height Setting amp mm in the upper part of the screen you can find tabs showing which main function is active or which are accessible directly 26
56. to the following e Perform a Blank Measurement in case the instrument is not used for a specific time e Enter sample and or patient data e Apply the sample for analysis e Print results either one by one or in groups by selecting records from the database Perform simple weekly maintenance as described later in this description 7 1 1 2 4 Calibration and Quality Control Abacus junior vet 5 arrives at your laboratory factory calibrated and ready to use However calibration needs updating whenever you find that the results have slightly changed or a different or new control material is used With each control material you receive to be used with the instrument you will find a control sheet listing the parameters the instrument should match Perform these calibrations as explained in a later chapter 7 2 Quality Control is used for checking for proper calibration and performance of the analyzer Running these samples should happen on a regular basis as also explained in a later chapter 7 3 1 3 Instrument features Figures 1 and 2 show front and rear view of cAbacus junior vet 5 hematology analyzer 1 2 3 4 9 6 7 8 9 Figure 1 Front view USB connector OK key Numerical keypad Function keys Graphic liquid crystal display HELP key Measure function key Database function key Utilities menu key Printing function key Exit menu key Cursor control keys Status indicator
57. tube with a higher lower bottom you can control the sampling height adjusting this option Needle offset is displayed in the lower left corner of the measurement screen Attention If you hurt yourself during analysis biohazard substances can cause infection Always use rubber gloves initiate analysis 1 Invert the closed sample tube 11 times to achieve a homogenous sample Do not shake the sample because micro bubbles can form inside which may cause erroneous sampling You have the possibility to use 3 different interchangeable adapters for different tube types Tube types are shown in the next figures Vacutainer with sample blood Sample tube with 5 ml control E Please wipe the mouth of the tube because the bursting bubble can dirty the instrument with blood which can cause measurement error at next sample Figure 8 Tubes used in big adapter Below you can see 3 types of tubes microtainers used in micro adapters These are only examples given by us you can try to use other type of microtainers as well Be careful to place the tube with the cap always in the position shown above Figure 9 Tubes used in micro adapter 37 2 ml control blood Figure 10 Tube used in small adapter 1 Remove the cap It is very important because the tip will not pierce the cap 2 Position the sample tube in the sample rotor 3 Push START key The sample rotor will turn into inside of the instrument and the needl
58. ule and a foil keypad of 29 keys including 6 software buttons with icons 6 function keys above LCD and has a START button The instrument allows sending results to an external printer or can have an optional built in printer module Its internal memory is capable of storing 2000 records with full histograms and individual patient data QC measurements can also be performed and stored The software operating the instrument is easy to upgrade using a USB drive The instrument allows connecting to a host computer for uploading records stored in the memory through a USB SLAVE port or serial link and also enables archiving and restoring records to and from USB drive NOTE If the equipment is used in a manner different from which the manufacturer specified the protection provided by the equipment may be impaired Misuse of equipment or use other than its intended purpose will invalidate conditions of warranty The accuracy and precision may also be impaired 1 2 1 Patient Testing The analyzer can process 24 30 samples per hour in 3 part mode 16 20 samples per hour in 5 part mode Samples can have individual sample data and additional parameters Results can be printed on the optional internal or external printer The print out format can be customized by the user Abacus junior vet 5 determines 22 hematology parameters including five part WBC differential in 5 part mode The instrument requires 50 ul 2 x 25 of the whole
59. ws model S N software version Displays operation statistics Tests the 5 5 internal systems Dialog for viewing modifying printer settings Dialog for setting screen saver delay VetScan date format and printing combined VetScan VET5 results Dialog for setting unit preferences Dialog for laboratory information used in report header Selects single or multi user mode Each user has unique user name ID and password Dialog for setting date time amp format Enables and disables reagent sensors Calibrates reagent sensors use if unit incorrectly reports missing reagents Displays contact information for technical service 3 Printer 1 Shut down 2 Preparing for shipment Prints selected test results Shuts off the VET5 use if the unit will not be used for over 72 hours Drains and shuts off the VET5 use if the unit will be unused for more than 2 weeks or if it will be shipped 22 4 OPERATING PRINCIPLES 4 1 Impedance Method The impedance method a k a Coulter method counts and sizes cells by detecting and measuring changes in electrical impedance when a particle in a conductive liquid passes through a small aperture puc poe Internal electrode Aperture S PLA EM pec sta dus oe Blood cell suspension External electrode Figure 7 Impedance method Each cell passing through the aperture there is a constant DC

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UM AJV5 01 1060

UM-AJV5-01-1060

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