UNISENSE SENSORTRACE SUITE
Contents
1. 35 PROFILING TAB 37 MOTOR CONTROL 37 PROFILE SETTINGS 38 PROFILE INTERACTION 40 PROFILE SELECTION 41 MANUAL PROFILING 41 VISUALIZE TAB RUE OCA Dre RR sommes eae DER OP UE 42 FILTERS 42 PROFILE SELECTION ACTIVITY TAB vs aco aote oa n oe E a SETTINGS AND ANALYSIS Ds AND THETHA SETTINGS Ds AND THETHA CALCULATE Ds AND THETHA TABLE Ds AND THETHA ANALYZE STATISTICS COMMENTS TAB eripere ket Vea ea e e nea IR Ee UI ids OUTPUT FILE REFERENCES aa wie RP QE V RU TROUBLESHOOTING 43 45 46 49 49 50 50 50 51 55 56 CONGRATULATIONS WITH YOUR NEW PRODUCT SUPPORT ORDERING AND CONTACT INFORMATION If you wish to order additional products or if you encounter any problems and need scientific technical assistance please do not hesitate to contact our sales and support team We will respond to your inquiry within one working day E mail sales unisense com Unisense A S Tueager 1 DK 8200 Aarhus N Denmark Tel 45 8944 9500 Fax 45 8944 9549 Further documentation and support is available at our website www unisense com WARRANTY AND LIABILITY Unisense SensorTrace Suite software is checked and validated on the operating systems as giv2. J x 9 x D x Sediment biofilm etc Depth x 1 The mass flux e g the diffusive O2 uptake can be calculated from the linear approximation to the concentration profile in the diffusive boundary layer DBL see figure using the one dimensional mass conservation equation Ficks 15t law p J Where J is the mass flux mol cms 4 is the change of concentration over the distance z in the DBL and Dg is the molecular diffusion coefficient in water 2 Justbelow the analyzed samples a straight line just below the surface interface the mass flux can be calculated from the straight concentration profile Ficks 15 law using following equation 13 dC dz Here D Dox alternative Ds Dg x 2 or Ds Do 143 x 1 where Ds is the mass transport coefficient and is the porosity of the sample J D X 3 The volume specific consumption and production can be determined from the shape of the concentration profile using Ficks 274 law of diffusion R D X vol S dz where R is the net rate nmol cm3 5 1 of production positive values or consumption negative values SensorTrace Profiling activity model uses the steady state concentration profile to 1 Calculate the diffusive mass flux 2 The volume specific activity rate for different depth intervals and the integrated production or consumption Before starting the analysis model you must provide estimates for the di
3. UNISENSE UNISENSE SENSORTRACE SUITE SENSORTRACE PROFILING USER MANUAL SENSORTRACE SUITE V2 1 User MANUAL Copyright 2015 Unisense A S Version January 2015 SENSORTRACE PROFILING USER MANUAL UNISENSE A S TABLE OF CONTENTS CONGRATULATIONS WITH YOUR NEW PRODUCT 6 SUPPORT ORDERING AND CONTACT INFORMATION 6 WARRANTY AND LIABILITY iuste sri 7 OVERVIEW Kean 9 RATE CALCULATION FROM CONCENTRATION 11 BACKGROUND 11 THEORY 13 SYSTEM FEATURES it c ce UII OR 15 INSTALLING THE SOFTWARE 44 20 rr RU Ee rw Ra xe 16 GETTING STARTED 6 KNN qa ee AR CCS 17 SETTINGS Deo edes esp pattes der dried eren ex dede 19 GENERAL FEATURES mamma per or VE VDO 22 LIVE DATA GRAPH esses zer xe PE nw diane DUE OPI ade 24 EXPERIMENT OVERVIEW 26 SENSORS AND 5 27 SENSORS 27 MOTORS 27 CALIBRATION TAB ierra tt epe e eo PEG 30 CALIBRATION OF OXYGEN 5 33 MOTOR SETTING
4. 12 13 Profile data 14 15 O Sensor 1 16 Profile 5 1 17 Depth um Concentration uM Raw mV 18 1500 279 165802 153 402359 19 1450 281 9421997 154 929718 20 1400 279 8255005 153 7653656 21 1350 278 1409912 152 8393555 PROFILE SELECTION The profile selection gives an overview of the profiles plotted on the graph Here you can check and uncheck the profiles you want to see on the graph See also page 41 Export figures The figures in the Calibration Tab Profiling Tab Visualize Tab and Activity Tab can be exported by right clicking the figure and choosing EXPORT AS IMAGE 45 ACTIVITY TAB SETTINGS AND ANALYSIS For background information and the theory behind the Activity calculation see Rate calcultaion from concentration profile starting at page 11 Settings and Analysis Boundary Conditions Intervals and zones Sensor 1 Bottom conc bottom flux 7 PME NEHME 2 000 5 Select profile 7 Bottom conc Bottom flux End depth um Min width 0 00 0 00 2000 Select analysis Sensor and profile In the Sensor and Profile fields you choose the sensor and profile you wish to analyze This will display the selected profile in the graph window Note The analysis can only be performed when the concentration is measured in umol L and if the sensor has been calibrated before measuring the profile When you have made an analysis you can view the result under SELECT ANALYSIS or by
5. 1 12128101 1096543 4 374 05 1119 85 8 97 1 01428688 0 04297115 1 01428688 7741 35 0 09098762 109183 8 97 172691331 0 03087907 1 72691331 6559 36 0 30391594 1088 55 897 1 86175294 0 0691942 1 86175294 For each zone number a row in the table is created with the following parameters Zones The number of zones in the calculation SSE Sum of Squared Errors is the difference between the simulated and observed profile The smaller this number the better 51 P Value the probability of the hypothesis that including an extra zone significantly improves the prediction The hypothesis is calculated based on the F value see Berg et al 1998 Top conc Calculated top concentration in umol L Bottom conc Calculated bottom concentration in umol L Top flux Calculated top flux in nmol cm 5 1 Bottom conc Calculated bottom flux nmol cm s Integrated prod Calculated integrated production in nmol cm s Volume specific rate The volume specific consumption or production rates nmol cm 5 1 are shown when moving the curser over the green or red rate bares in the activity plot The values are also shown in the exported file selection nr 1 Export data point and selection row in table see below When all calculations are done the solution with the highest number of zones that is significantly better than the previous solution P 0 05 will be the best choice However it is advisable to visually check the other estimated
6. Uncheck all O Sensor 1 Profile 2 1 5 sensor 1 Pr x g O Sensor 1 Profile 4 1 x 0 y 0 El Sensor 1 Profile 5 1 x 0 y 0 1 0 a Ke ip EAN Pa MANUAL PROFILING If you do not have a motor unit you will have to move the sensors manually with a micromanipulator In this case a dialog box will appear after each measurement that will tell you which depth to go to When you have reached the depth appearing in the dialog box press OK The program will use the time indicated in Wait before measure measure period and replicates before a new dialog box will appear 41 VISUALIZE TAB This tab is for advanced visualization of the measured profiles You can plot one or more profiles and you can choose to see the profiles of one sensor or multiple sensors The zero depth can be adjusted and the new adjusted profile can be saved and be used in the Activity tab FiLTERS Filters Sensor O Sensor 1 Cycle All Z Depth X Position Y Position Plot Profile Adjust depth Reset depth m Calibrated m Standard deviation Export profiles Sensor Here you choose the sensor or sensors you want to see the profiles from Cycle It is possible to choose how many cycles you want to see on the graph In a 1D setting using only z depths one profile is identical to one cycle For 2D and 3D cycle the profile of one or more profiles in one cycle or multiple cycles will be plotted in a 1D plot In a 2D s
7. Velocity Acceleration Port connected Lab Motor MC 232 Disabled v 1000 1000 COM30 Lab Motor MC 232 X v 1000 1000 COM30 Lab Motor MC 232 Y v 1000 1000 COM30 Field Motor 7416 Z v N A N A Motor 1 Field Motor 7416 Disabled v N A Motor 2 Manual motor If a manual micromanipulator is part of the experimental setup the manual motor setting should be selected and the user will be prompted every time the sensor should be moved between measurements 20 After the Settings have been checked and adjusted accordingly press the START EXPERIMENT button The software will subsequently make several tabs available The sensor and motor configuration will be saved Note that settings cannot be changed after starting the experiment 21 GENERAL FEATURES The SensorTrace Profiling application is by default divided into four main areas 1 Tab selection 2 Live Data Tab 3 Experiment overview Tab and 4 Sensors and Motors The tabs can be pinned or unpinned depending on whether you wish to have a permanent view of the tab It is possible to move each of the four tabs and place them on the screen where you want To move them pin the selected tab so it is permanently visible Drag the tab where you want to have it by using the mouse The tabs can be moved back to their original position using the arrows that will appear in both sides of the screen and in the middle Place the pane on the arrow that represents the direction for the place
8. based on measured concentration profiles After the experiment all measurements can be exported in a text file csv for easy handling and analysis in Microsoft Excel 15 INSTALLING THE SOFTWARE Make sure that you are installing having the full administration rights Start the installation program exe file from the CD Follow the instructions given by the installation program This will install the SensorTrace Suite program including a version ofthis manual in the Knowledge Base of the program The Instacal program must be installed on the PC in order to use SensorTrace Suite Instacal is install as part SensorTrace Suite and its install file is by default placed C Program Files x86 NUnisenseV SensorTrace Suite Drivers InstaCal Install exe The program will be placed in a program group called Measurement Computing after instillation To activate the full SensorTrace Suite program and gain access to Logger Photo Rate and Profiling enter the License Key supplied with the installation CD To access the dialog box for entering the license key click on the ENTER License Kev button in the lower right corner of the main SensorTrace Suite window Press ACTIVATE when the key is fully entered Contact SALES UNISENSE COM to purchase a license key Register product Enter the license key to active the product License key E GETTING STARTED Set the PC power management to Always ON And make sure that your
9. is reached during a profile the system will wait for a period of seconds before it starts measuring This is to ensure that the sensor signal is stable before the measurements start The default setting is 3 seconds Measure period s sets the duration of the measurement in each position Each measurement will be an average value over this period of time When making profiles in a noisy environment such as on a ship or in a cold room it can be helpful to average over a longer period i e increase the measure period The default setting is 1 second but should be set to match the measuring condition The standard deviation for the values are shown in the visualize tab when tagged Delay Between s is used when starting a profile and during repeating cycles Each time a cycle is started the sensor is place in the Safe position and the profile is started after a delay period given here 39 Replicates Sets the number of measurements that should be performed at each depth Number of cycles Set the desired number of cycles here In a 1D setting a profile is identical to one cycle In a 2D setting one cycle includes all the profiles measured along the x axis whereas in a 3D setting one cycle includes all the profiles measured along both the x axis and y axis EXAMPLE SETTINGS FOR PROFILING WITH A 50 uM SENSOR START 1500 um WAIT BEFORE MEASURE 3 s END 4000 um MEASURE PERIOD 1 s STEP SIZE 50 um REPLICATES 1 SAFE 4000 u
10. processes in neighboring layers The model assumes steady state conditions where transport of solutes only occurs by diffusion In many samples e g sediments and biobilms the assumption is valid however if your system is heavily affected by pore water movements due to burial groundwater flow wave action and similar movements the SensorTrace Profile activity model should be used with care 11 Depth x Concentration Consumption Bulk water phase Diffusive boundary Straight line layer DBL Surface diffusion Concave curve production Sediment biofilm etc Convex curve consumption Depth x RULES OF THUMB If the diffusion coefficient and porosity is constant in a layer the following rules of thumb can be used for a qualitative interpretation of the processes in that layer Ifthe concentration curve is convex there is net consumption e g respiration oxidation of reduced compounds 1 concentration curve is concave there is net production e g photosynth enthetic production of oxygen sulfide production by sulfate reduction If the concentration curve is linear there is no net consumption or production only diffusional transport THEORY Under steady state conditions the activity can be calculated from microprofiles by different approaches see e g Glud 2008 Bulk water phase Diffusive boundary layer DBL dC x Concentration Consumption dx
11. the following topics User Master Experiment Experiment name type New Experiment Settings Profiling Experiment Name Researcher Name Experiment Description Master Experiment Sediment Create new Master experiment and Created Date To easily find an experiment it is possible to sort the experiments by opening the dropdown window of the topic bars Press LOAD when the experiment is selected The Old Experiment mode is for working with old data settings and parameters cannot be changed and new measurements cannot be started Press CREATE aster Experiment name SETTINGS TAB The first tab to appear is the Settings tab This tab will display the detected hardware and sensors In the Sensors table various parameters for the sensor s can be chosen The software will automatically start searching for connected instruments e g Unisense Microsensor Multimeter Field Microsensor Multimeter or motor controllers If no instruments are recognized it is possible to manually repeat the scan In the settings window the registered sensor channels are found at the top For each sensor channel there are several setting options From left to right you can adjust the following information Selection of the sensor channel sensor type sensor measuring unit output range and sensor name Furthermore it is possible to add a short comment to each sensor a Sensor Mark the checkboxes for the channel sensors you
12. want to view and record signals from Type Choose sensor type from the drop down menu if the default value is not appropriate Unit Select an appropriate concentration unit for the sensor signal when calibrated 19 Range V Select the voltage range for the amplifier Select the smallest range possible to get the most out of the resolution of the amplifier matching the expected signal range of the sensors It is recommended not to select an unnecessary high range as this may cause a loss in resolution However the range should not be chosen so small that the signal gets beyond the selected range This will cause the amplifier to get saturated Name Write a name that describes your sensor optional Comment Write a comment about your sensor optional Motors The number of connected motors detected by the program are shown at the bottom of the window By default velocity and acceleration are set to 1000 If a Field Microsensor Multimeter is connected the two motor channels will be shown even if no motor is connected The acceleration of the Field Motor can be set on the Field Microsensor Multimeter If a 3 D motorized system is used motors Z X and Y should be recognized For 1 D system only motor stage Z will be identified Both the laboratory motor and the field motor can be used in a combined 2 D or 3 D motorized system Here you define which motor should act as Z X and Y Scan motors and configure them Device Motor Axis
13. PC does not enter sleep mode or stand by during measurements as this will interrupt the connection to the instruments and it will be necessary to restart the program Connect all instruments in your set up to the computer Start SensorTrace Suite it is by default placed on your computer under Programs in the Unisense folder The following main program window appears SensorTrace Suite UN Desin Choose either to make a new Profiling experiment or load an old experiment a New Experiment When a new experiment is selected you will be asked to create a new experiment from the dialog box that appears Create the new experiment by naming experiment and researcher identities followed by an optional brief experimental description Finally select an exsisting IMPORTANT Please make sure that your PC does not enter sleep mode or stand by during measurements as this will interrupt the connection to the instruments and it will be necessary to restart the program 17 master experiment or create a new master experiment The master experiment allows you to group several sub experiments e g on the same sample or sample station Press CREATE Load or delete Experiment experiments obtained by SensorTrace Profiling are stored in the SensorTrace Suite database Press PROFILING under Old Experiments A dialog box appears were you can choose the experiment you want to open or delete Experiments are grouped by
14. alibration program can correct the O2 saturation concentration based on knowledge about the humidity By default 10096 humidity is used Calibration High Calibrate the MicroOptode by inserting the optode and the temperature sensor into oxygen saturated solution 10096 air saturation as described in the MicroOptode manual When a stable signal is obtained click the CALIBRATE HIGH button Calibration Low Insert the MicroOptode and temperature sensor in anoxic solution 096 Oz and click the CALIBRATE LOW button when the signal is stable As with all other sensors the green check mark indicates that the MicroOptode is now calibrated If you need to make a new calibration or you made a mistake press DISCARD CALIBRATION Status In the status bar you find information on the sensor If the sensor and reference signals are normal the status bare shows a green OK box When the sensor signal is low e g because no sensor is connected the fiber optic is broken or the sensor dye has fallen of the tip the status bar will turn red and give the text Sensor signal is too low Status Sensor signat Reference signal 34 MOTOR SETTING The manual or motorized movements of x y and z axis are indicated here The program will automatically set MOTOR when it recognizes a motor A manual movement is performed if set on MANUAL If a motor is not connected or found by the software it will automatically set NONE Pin the Sensor
15. and Oceanography 43 7 1500 1510 Glud N R 2008 Oxygen dynamics of marine sediments Marine Biology Research 4 243 289 e Iversen and Jorgensen 1993 Diffusion Coefficients of Sulfate and Methane in Marine Sediments Influence of Porosity Geochimica et Cosmochimica Acta 57 3 571 578 Ullman and Aller R C 1982 Diffusion Coefficients in Nearshore Marine Sediments Limnology and Oceanography 27 3 552 556 Revsbech Nielsen L P and Ramsing N B 1998 A Novel Microsensor for Determination of Apparent Diffusivity in Sediments Limnology and Oceanography 43 5 986 992 57 TROUBLESHOOTING Problem Possible cause 1 Possible cause 2 Solution Problem Possible cause 1 Solution Possible cause 2 58 Solution Instruments not found You have not connected your digital sensor instrument or A D converter An amplifier with different bit resolution has just been connected Open the configuration utility InstaCal InstaCal will clear any old amplifiers not connected to your system and search for connected amplifiers Noisy measurements Physical vibrations from other appliances on the table are causing movements of the sensor resulting in instability of experimental set up and disturbances in the measurements Remove all unrelated appliances from surfaces in contact with the experimental set up and ensure completely stable conditions for the sample Elec
16. bration data and figure See Visualize Tab on page 42 32 IMPORTANT When retrieving a calibration make sure that the retrieved calibration matches your current sensor in terms of signal size and units and that the calibration temperature etc are still valid CALIBRATION OF OXYGEN MICROOPTODES Live data for oxygen MicroOptodes are first shown after a calibration has been made A fast calibration can be made to get a live view Afterwards continue to make the real calibration w Oz Sensor 2 Temperature sensor Temperature Sensor 1 x Optode type 430 um brown v Salinity Humidity 10 Status Sensor signal Ok Reference signal Sensor In the top menu you can select the sensor you want to calibrate A red cross indicates that the sensor has not been calibrated green tag indicate that the sensor has been calibrated Temperature sensor Select CONSTANT USER DEFINED and add the temperature if the optode is to be used at a constant known temperature If a temperature sensor is connected select the TEMPERATURE SENSOR of the recording sensor Optode type Add the type of MicroOptode sensor to be calibrated The color description refers to the color of the fiber wire Salinity Add the salinity of the calibration solution The salinity should match the salinity of your sample 33 Humidity Add the amount of humidity in air used to obtain an oxygen saturated solution The optode c
17. cking the CALIBRATED checkbox for each graph If no calibration has been performed and the checkbox is checked no signals will be plotted Pause By clicking the PAUSE check box the auto updating is halted and zooming and scrolling through the data is easier Data is still logged and un checking the Pause will update the graph Y zoom by checking the Y xoom checkbox the graph will be zoomed only on the y axis and not on the x axis Show last By default the time scale x axis is controlled in the SHOW LAST drop down list where a number of preset time intervals can be chosen Chart legend At the top of the Live Data there is a sensor legend showing the graph color for the associated sensors and their current signal concentration value Datapoints The datapoints in the Live Data window can be cleared by pressing CLEAR This will NOT affect the stored data ZOOM FUNCTION Itis possible to zoom in and out on the graph by using the mouse wheel Point the curser on part of the graph of interest and use the mouse wheel to zoom in and out A certain time span drag a rectangle with the mouse from the upper left corner to the lower right corner Fast zoom out double click on the graph 25 EXPERIMENT OVERVIEW The Experiment overview shows all the profile experiments profiles and analysis made in an experiment The tab is always visible if pinned and can be hidden when not pinned Experiment overviev 4 Profi
18. crooptode Meter 3 Temperature Temperoture BipolarsVolt Temperature Sensor 1 n ssa 15 start Enddepth um zones Min zone width um Boundary condition 16 o 1150 5 100 Bottom conc bottom flux v 18 Szones 19 20 start End Activity nmol em 3 s 1 E o 158 2214794 1240974026 EI 1582214790 321 5215604 193554375 z 321521562641 2138101 1515291533 6an2138101 96020657 6040557208 1150000021 pec 27 Statistics a 29 Zone SSE P value Top Concentration umol L Bottom Concentration jmol l Top Flux nmol 2 5 1 Bottom Flu nmol cm 2 5 1 Integrated ped nmol cr 30 gt 1559 35 030391394 E t E 32 Observed Profile Simulated profile 33 Obs Concentration gM Obs Depthlge Sim Concentration yM 0 as 281210938 1500 o 36 282 4198914 1450 1092 664484 10 1 37 283 055603 1400 110068255 3 858060473 53 2 EXPORT ENTIRE RESULT TABLE You will save calibrations comments and statistics for all zones For references please see the References section in the back of this manual Choose how to export data O Export Data points and selected row in table O Export entire result table ok NOTE The application uses as separator E A l B D i Product version Database version 2 1 0 5 26957 12 Bm 4 Devices used 5 26 Instrument name Channel Type Unit Range Sensor name Comment 7 Oxymet
19. double clicking the analysis in the Experiments Overview Boundary Conditions Specify boundary conditions It is possible to choose between several different boundary conditions see Berg et al 1998 To choose the right set of conditions it is important to consider the characteristics of the profile see examples in Boundary condition examples box Concentration and flux numbers for the boundary condition should be added 46 BOUNDARY CONDITION EXAMPLES BOX Oxygen profiles in many cases end with a constant concentration of zero as all the oxygen is used up at the bottom of the profile This implies that there is a zero concentration and also a zero concentration gradient at the bottom of the profile and the boundary concentration BOTTOM CONC BOTTOM FLUX is appropriate with zero as the parameters entered in the boxes below For sulfide profiles on the other hand the concentration is typically zero over an interval at the top of the profile and consequently also the flux is zero so in this case CONC FLUX is appropriate For flux calculations in the DBL BOTTOM CONC is selected and the concentration at the top of the DBL and the conc at the sediment surface are added 47 Intervals and zones Choose the START and END DEPTH of the profile where the calculation should be performed Then choose the maximum number of zones in MAX ZONES to be used in the calculation 1 10 zones can be sel
20. e text first wait for an expected event and then update the time Settings Calibration Motor settings Profiling Visualize Activity Time type comment 05 07 2014 10 09 31 StartExperiment 05 07 2014 10 10 13 Calibration Calibrate O Sensor 2 05 07 2014 10 11 31 Calibration Calibrate O Sensor 2 05 07 2014 10 35 58 Calibration Calibrate O Sensor 2 05 07 2014 10 39 28 Calibration Calibrate O Sensor 2 05 07 2014 10 41 13 Calibration Calibrate Sensor 2 05 07 2014 11 43 10 Calibration Calibrated Sensor 1 05 07 2014 12 27 28 Profiling The zero depth has been reset 05 07 2014 12 57 43 Calibration Water Use current time 8 Water 55 OUTPUT FILE All logged data and text entered and stored in the SensorTrace Profiling program are logged to an internal SensorTrace Suite database Data including raw data are accessible through data export in an Microsoft Excel compatible output file format CSV to facilitate processing and graphic representation of the data All you need is to make sure that you have a program that can view or import CSV files e g Microsoft Excel Raw and calibrated data and the calibration values can be exported from the Visualize Tab page 42 whereas the rate values calculated in the Activity Tab are exported from this tab see page 46 56 REFERENCES Berg P Risgaard Petersen N and Rysgaard S 1998 Interpretation of measured concentration profiles in sediment pore water Limnology
21. ected Note that the maximum number of zones multiplied with the minimum zone width has to be smaller than the distance between the start and end points The min width is the smallest zone width that the method allows and it should not be smaller than twice the step size in the profile This will typically be the top of the sediment and the end of the profile The number of zones giving the best solution can vary between profiles INTERVALS AND ZONES EXAMPLE BOX Diffusive mass flux in the DBL Equation 1 in the Theory section Start depth is the top depth of the boundary layer End depth is the depth of the sample surface Max zones is one Do is used for this calculation Notice that a mass flux calculated from the DBL can only be recommended when sufficient data points are available from the DBL e g by using sensors with tip size lt 50 um For volume specific and integrated consumption and production rate calculations Equation 2 and 3 in the Theory section page 13 Start depth is the depth of the sediment surfaces End depth is the depth where the profile ends and Max zone between 1 and 10 Ds values are used for these calculations 48 Ds AND THETHA To make the calculation it is necessary for the program to have values for diffusion coefficients and porosity These values can either be measured values obtained by different methods see e g Ullman and Aller 1982 Iversen and Jorgensen 1993 Revsbech et a
22. en in the specification running English language settings It comes with lifetime updates Software must be installed under administrator rights Customer must ensure PC is fully updated and no conflicting third party software is installed Unisense do not warrant compliance with any other operating systems language settings or third party software For instrumentation and sensors please refer to our warranty conditions as given in the document General Terms of Sale and Delivery of Unisense A S found on www unisense com LICENSE AGREEMENT The following terms shall apply to the software provided by Unisense A S Unisense in connection with the simultaneous sale to you Customer of a Unisense SensorTrace Suite Software All rights title and interest in the software belong to Unisense Unisense grants to the Customer a royalty free non exclusive and non transferable license to use the software solely in connection with the Unisense Product purchased from Unisense simultaneously with the purchase of the software The Customer undertakes not to copy modify reverse engineer disassemble or de compile all or any part of the software or rent lease distribute or sell the software The Customer shall however be entitled to make one copy of the software for back up and recovery purposes for use solely in connection with the Unisense Products supplied by Unisense together with the software Nothing in this License Agreement or any oth
23. ensor The sensor signal can also be followed continuously in the Live Data graph at the bottom of the screen bxc sooo 2 Sensor value 020 mV OD Caton Imported Cal Pointe Time Slope Intercept R Comment 3 15 07 2014 09 40 21 0 005 0316 0449 Calibrated Sensor 1 Calibration procedure 1 Prepare the calibration samples 2 Choose the sensor you want to calibrate 3 Change the concentration in the concentration box according to the actual calibration solution For oxygen an automated procedure to calculate the atmospheric saturation as a function of temperature and salinity can be invoked by pressing the button named CALCULATE O2 30 The below dialog box will be shown Add the temperature and salinity of the calibration solution to receive the oxygen concentration Press APPLY Calculate oxygen at air saturation Temperature C 2200 Salinity o 000 Concentration 272 972 umol L After entering the correct concentration add the calibration point by pressing ADD POINT Several points can be added for each concentration Change to another calibration standard and repeat points 3 4 It is possible to use several different standards and make a multi point calibration to verify linearity If a calibration point is not valid e g due to typing errors a single point can be cleared by selecting it with the mouse and pressing DeLeTe Point Al
24. er S N 6061 A 02 umoll BipolarSVolt 1 8 _MicroOptode Meter 102 umoll BipolarSVolt Sensor 2 9 MicroOptode Meter 202 umoll Bipolarsvolt Sensor 3 10 MicroOptode Meter 3 Temperature Temperature BipolarSVolt Temperature Sensor 1 mj 13 Settings 15 Startdepth um Enddepth um Min zone width um Boundary condition 16 o 1150 5 100 Bottom conc bottom flux 17 18 Statistics 19 20 Zone SSE P Value TopConcentration ymol L Bottom Concentration umol TopFlux nmolcm 2s 1 Bottom Flux nmol 25 1 Integrated pod nmol cm 2 s 1 2 1 2656852 78 0 1757 76 8 97 1 77112765 0 1 77112765 2 2 21643774 9 89 05 1083 67 897 1 12128101 0 02862094 1 12128101 23 3 10965 43 4 37 05 1119 85 8 97 1 01428688 0 04297115 1 01428688 24 4 7741 35 0 09098762 1091 83 897 1 72691331 0 03087907 1 72691331 25 5 6559 36 0 30391594 1088 55 8 97 1 86175294 0 0691942 1 86175294 26 54 COMMENTS TAB Comments calibrations and other events generated by the user or the program are recorded in the Comment tab The tab allows you to enter notes and comments Any text that you want to save with your data e g a general description of your experiment can be entered Text entered in the Profiling tab and Calibration tab are also listed in the Comment tab All activities can also be seen as colored marks in the Live Data window A comment can be added using 1 the current time or 2 by writing th
25. er agreement between Unisense and the Customer shall be construed as an obligation for Unisense to provide to the Customer updates of the software This License Agreement shall automatically terminate if the Customer violates the terms of the license In case of termination of the license the Customer shall immediately destroy the software and any copy thereof THE CUSTOMER TAKES THE SOFTWARE AS IS UNISENSE MAKES NO WARRANTY OR REPRESENTATION CONCERNING THE SOFTWARE AND EXPRESSLY DISCLAIMS ALL OTHER WARRANTIES AND CONDITIONS EXPRESS OR IMPLIED STATUTORY OR OTHERWISE OF WHATEVER KIND OR NATURE INCLUDING BUT NOT LIMITED TO ANY AND ALL IMPLIED WARRANTIES INCLUDING IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE UNISENSE SHALL NOT BE LIABLE FOR ANY DAMAGES OF ANY KIND INCLUDING INCIDENTAL SPECIAL PUNITIVE CONSEQUENTIAL AND SIMILAR DAMAGES INCLUDING WITHOUT LIMITATION LOSS OF PRODUCTION LOSS OF PROFIT LOSS OF DATA LOSS OF GOODWILL LOSS OF CONTRACTS OR BUSINESS INTERRUPTION This License Agreement and any dispute arising out of or in relation to this License Agreement shall be governed by and construed in accordance with the laws of Denmark exclusive of its choice of law provisions The venue for any such dispute shall be the Danish courts provided however that Unisense shall be entitled to instigate legal proceedings against the Customer before the courts with jurisdiction over the matter located
26. etting one cycle includes all the profiles measured along the x axis whereas in a 3D setting one cycle includes all the profiles measured along both the x axis and y axis 42 NOTE Update new filter setting by choosing Profile This will plot the new graph Z Depth This setting has currently no function because SensorTrace Profiling cannot make contour plot It will be available in future update X Depth and Y Depth Profile plots from a defined x position and y position can be chosen here 3 i Profile Pressing profile you will see the graphs chosen according to your filter setting All profiles including profiles in 2D and 3D dimensions will be plotted in a 1D graph SensorTrace Profiling can currently not make contour plot Adjust zero depth On the graph it is possible to change the zero depth position by dragging the red horizontal line to the new zero depth position Pressing Adjust zero will adjust only the profiles shown on the graph The original depth position is re found when pressing REsET DEPTH The zero depth adjustments will be saved into the Activity Tab Analysis can be made in the Activity Tab with the adjusted zero depths and be saved as an analysis However only the original depths are saved for future data handling 43 Calibrated When checking CALIBRATED the calibrated values are visible on the graph Standard deviation Checking STANDARD DEVIATION the graph will disp
27. ffusion coefficient and the porosity in all zones and some boundary conditions see Activity Tab The model makes an initial guess of the activity distribution and compares the calculated profile with the actual measured profile Using a stepwise optimization method the activity distribution is refined until the calculated profile does not deviate from the measured profile within some statistical margin Statistical values like the sum of squared error and the P value together with the modeled graph will help you to estimate the best fit for the activity calculations SYSTEM FEATURES SensorTrace Profiling is a program for logging a series of measurement points with a microsensor or microoptode along path A detailed overview of the components for a Unisense profiling measurement set up can be found in our Profiling System User Manual Basic features of the program are Control of settings for profile start ups motor run and data logging Calibration settings are saved between profiling for easy set up of next profiles The program keeps track of measurements collected in different profiles and can show them simultaneously for comparison Multiple sensors can be used simultaneously Raw data and results are continually saved for later data interpretation Graph view of measurements obtained by one or more sensors during and after profiling The possibility to model the distribution of production and consumption
28. g can be found in the literature Sediment diffusion coefficient equation Ds Select an equation to calculate the sediment diffusion coefficient with depth from the porosity in the box See a discussion of the most suitable equation in Ullman and Aller 1982 and lversen and J rgensen 1993 Ds AND In the table you can manually set the start and end depths porosity and calulate the diffusion coefficient Ds Diffusion coefficient Ds Ds in the table is calculated in the Calculate section see above using the porosity set in the table and the diffusion coefficient in free water Do To use independent diffusion coefficient values type diffusion coefficient values measured or estimated directly in the table ANALYZE Press the ANALYZE button to start the activity analysis The calculation starts automatically by first performing the analysis with one zone and then makes the calculation for increasingly high zone numbers until the specified maximum zone number 50 Rate nmol em 3 s 150 am 50 50 eres o i 00000 0000 US m Depthtum 1000 11 2900 STATISTICS Statistics Save solution Export data data P Value Top Conc Bottc Top Flux Bottom Flux Integrated prod umol L nmol cm 2 s 1 nmol cm 2 5 1 nmol cm 2 5 1 265685278 0 175776 8 97 177112765 177112765 216437 74 9 889 05 1083 67 8 97 1 12128101 0 02862094
29. he Profiling tab controls the z axis depth of your profile For x IMPORTANT and y setting see Motor setting page 35 and Sensors and Motors The depth page 27 scale is positive downwards Profiling with depth can be done with or without a motor unit motorized or manual The functions of this sheet are the same whether you do your profiles manually or motorized aee caseum Mattin ___ Atty Gm egeret mmm MOTOR CONTROL Here you can read adjust and redefine the sensor position of the sensor in use Pin the Sensors and Motors tab and control the sensor position using the motor Z setting Move the sensor position up and down using Move by step Define the Home depth of the sensor tip e g set New Depth at 0 um when the sensor tip is placed at the surface of the sample see also Sensors and Motors page 27 37 PROFILE SETTINGS In this setting you define the profile r Profile settings Start um Wait before measure s 2 000 3 00 End um Measure period s 2000 100 Step size um Replicates 50 105 Safe um Delay Between s 4000 100 Sensor angle Number of cycles 0 i Start um is the depth position relative to the Home depth from where the profile is started Negative values are above the surface and thus normally the start position should be negative End is the depth position relative to the Home depth whe
30. in a country where the Customer has a place of business or is incorporated or organized OVERVIEW SensorTrace Profiling is the profiling program from the Unisense program SensorTrace Suite It offers time series data logging along a path it can visualize these measurements and calculate activity rates The software supports motor controlled automated measurements but also manual positioning of individual sensors SensorTrace Profiling is compatible with all Unisense instruments designed for use in laboratories The program automatically saves all data in an SOL database and all data can be exported in csv formatted files for subsequent data analysis System requirements 42GHz PC Windows XP Vista Windows 7 32 bit 64 Windows 8 8 1 Min 500 MB free hard disk space USB port s Min 4 GB RAM Min screen resolution 1280 x 800 Microsoft Excel or a program that can view exported files CSV files Unisense amplifier OXY Meter MicroOptode Meter Microsensor Multimeter Field Microsensor Multimeter or Microsensor Monometer or A D converter Other programs available in the full SensorTrace Suite SensorTrace Logger For basic data acquisition and motor control It offers time series data logging and calibration features we recommend this application SensorTrace Photo For photosynthetic experiments using the light dark switch technique we recommend this application SensorTrace Rate For microrespi
31. l 1998 or they can be estimates based on literature values Settines Ds AND Zones with different diffusion parameters Add the Number and borders of the zones with different diffusion coefficient and porosity First select the number of zones in the entry box Then adjust the start depth of each zone Default Porosity To specify a uniform porosity in all depths Enter a measured or estimated value and click POROSITY To enter variable porosity Enter measured or estimated porosity values by typing directly in the table If only a subset of the porosity values need to be different set a uniform porosity and modify the subset by typing in the relevant cells afterwards Settings Calculate Zones with different diffusion parameters 00 coefficient free water 10 5 cm2 s 1 22 873 Default Porosity Sediment diffusion coefficient equation 0S PorD Start End Porosity Diffusion coefficient 1500 0 1 8 73 05 0 1500 0 8 6 984 05 m Remember settings across experiments 49 CALCULATE Ds AND THETHA Dg in free water Enter the diffusion coefficient in free water Do at the actual salinity and temperature For oxygen the value is found by using Oz TABLE The Unisense table for values for oxygen diffusion coefficients is also available in the Documentation and Downloads section of our website http www unisense com For other solutes D
32. l calibration points can be removed by pressing CLEAR ALL POINTS When you are satisfied with your calibration press APPLY CALIBRATION A linear regression will be performed based on the calibration and this regression will form the basis for converting signals to calibrated values Values are displayed in the table If you have made more than one calibration the 31 program will use the last made calibration 8 Repeat 2 7 for other sensors The calibration table below the calibration graph shows the calibrations for the chosen sensor Each calibration will appear here with information on calibration number time of calibration linear regression data slope intercept and r2 as well as additional user comments that can be entered directly into the table All calibration data are stored in the SensorTrace Suite database Recalibration procedure Sensors can be recalibrated at any time during an experiment The new calibration applies from the time of calibration onward To recalibrate the sensor 1 Press CLEAR ALL POINTS 2 Follow step 1 6 in the Calibration procedure Retrieving a calibration If you want to use a different calibration highlight in the table the calibration you want to use and press RETRIEVE CALIBRATION The sensor will then be calibrated according to the new calibration Deleting a calibration If you want to delete a calibration highlight it in the table and press DELETE CALIBRATION Export cali
33. lay the standard deviation of the values measured in the time period given under measure period in the profile settings The standard deviations can be seen on the graph in its zoom out position and the numbers in the export file Export profiles Here you can export data from the profiles matched by the filter settings It is possible to export the profile date as one or two files Choosing EXPORT As 2 FILES A file containing Calibration and Comments and a file containing Data Choosing Export As 1 FILE A file Containing calibration comments and data If you have adjusted the zero depth then the profiles with the zero depth adjustment will be exported but not permanently saved If you want to export the original Depth values press RESET DEPTH before you EXPORT PROFILES Choose how to export data Q Export as 2 files A file containing Calibration and Comments and a file containing Data Q Export as 1 file A file Containing both Calibration Comments and Data EIXS NOTE The application uses as separator 44 EI A B G D E 1 F 1 Product version Database version 2 1 0 5 26957 12 3 4 Devices used 5 6 Instrument name Channel Type Unit Range Sensor name Comment 7 Oxymeter S N6061 A 02 umoll Bipolar5Volt Sensor 1 8 MicroOptode Meter 102 umoll Bipolar5Volt Sensor 2 9 MicroOptode Meter 202 umoll Bipolar5Volt Sensor 3 10 MicroOptode Meter 3 Temperature Temperature Bipolar5Volt Temperature Sensor 1 1
34. le experiment 2 Profile 2 1 4 Profile experiment 3 Profile 3 1 4 Profile experiment 4 Profile 4 1 4 Profile experiment 5 Profile 5 1 4 Profile experiment 6 4 Profile 6 1 Analysis 1 4 Profile experiment 7 4 Profile 7 1 Analysis 1 Profile experiment The program automatically saves all the profiles made in an experiment One profile experiment can contain one profile or multiple profiles repeated in one run defined in the Profiling tab These are stored as Profile 1 Profile 2 and so on It is possible to delete a profile by right clicking using the mouse and choose delete Analysis The analysis of a profile made in the Activity tab is stored as an Analysis under the studied profile If you make several they are stored under the same profile as Analysis 1 Analysis 2 and so on To open an analysis simply double click it It is possible to delete an analysis by right clicking and choosing delete 26 SENSORS AND MOTORS SENSORS The sensors for the profile measurement are selected here Sensors and Motors m Sensor 1 m H S Sensor 1 m pH Sensor 1 W Temperature Sensor 1 MOTORS This control box allows you to read adjust and redefine your sensor position Actual Depth um 4000 New Depth um Move by step um 7E Down The number of connected motors detected by the program are shown at the bottom of the window For a 1 D system only motor stage Z will be identified If a 3 D motorized microma
35. m DELAY BETWEEN 1 s SENSOR ANGLE 0 NUMBER OF CYCLES 1 PROFILE INTERACTION Here the profile is started and stopped at any time during profiling Profile interaction Status Ready Profile interaction Status Moving Z 1 1 Start Profiling When all parameters are set press START The data will be logged in the PC memory and when the profile is finished or stopped the data will be saved to the SensorTrace Suite database Pause profiling The same button is used to Start and Pause profiling as illustrated above Simply press PAUSE to pause the profiling Continue the profiling program by pressing START again 40 Stop profiling Stop the current profiling by pressing STOP This will stop the profiling program immediately and prompt the user whether the sensors shall be moved to the safe position and the X Y profiling to its start position Comments At all times during the experiment it is possible to enter a comment Enter the comment and time stamp and press ADD All comments are listed in the Comment tab Remaining cycles 15 a status reading on how many cycles the program still needs to run Calibrated Marking CALIBRATED will show the calibrated values in the profile graph PROFILE SELECTION A profile plot is shown while profiling During a profile run it is possible to show previous measured profiles Mark the profiles you want to view during the profile run a Profile selection m Check
36. ment Alternatively choose RESET LAYOUT in the windows tab 15100 151200 151300 151400 154500 22 NOTE You can move between the tabs at any time during profiling Tab selection 1 The upper area is divided into tabs which allow you to access different functions of the program From left to right the following functions are available Settings Calibrations Motor settings Profiling Visualize Activity and Comments Multiple tabs can be shown in one tab window by dragging one or more tab functions from the tab line into another tab The tabs can be moved back to their original position by dragging them back to the tab line Live data tab 2 The lower area the Live Data shows the live sensor signals of one or more sensors The height of the live data window can be changed by dragging its upper border Experiment Overview tab 3 In the left side area Experiment Overview lists the profile experiments Sensor and Motors 4 In the right side area Sensors and Motors show the sensor selection and if available the motor control All components will be described in detail on the following pages 23 LIVE DATA GRAPH The Live Data graph is permanently visible in the lower part of the SensorTrace Profiling interface It allows the user to view sensor signals continuously By default uncalibrated raw sensor signals are shown but if the CALIBRATED check box is checked calibrated values are plotted for calibra
37. nipulator system is used motors Z X and Y should be recognized For the X axis and Y axis motor the UP and DOWN movement refers to the movement relative to the motor position on the moving slide Going DOWN means the movement will go away from the motor 27 If a manual micromanipulator is part of the experiment setup a manual sensor movement can be included and the user will be prompted every time the sensor should be moved between measurements Actual Depth um indicates the current vertical position of the sensor tip NOTE the Actual Depth is arbitrary until the user has related the position of the sensor tip to the position of the study sample see the example below Set Home re defines the motor position Actual Depth um to the position given in New Depth um Go Home By pressing Go Howe the motor will place the senor tip at the position set as Home position New Depth re defines the motor position Actual Depth um to the position given in New Depth um GoTo The sensor can be moved directly to a defined depth compared to the New Depth position by pressing GoTo Move by step um Enter the step size the motor should move the sensor up or down 28 IMPORTANT The depth scale is positive downwards For example if the zero is set at the sediment surface positive values in the boxes below Actual Depth and New Depth will indicate that the sensor is in or should move down into the sediment All depth
38. ration experiments to measure the metabolic rates including respiration rates of small aquatic animals bacteria or oxygen production of phytoplankton we recommend this application RATE CALCULATION FROM CONCENTRATION PROFILES High resolution concentration profiles measured with Unisense microsensors can be used to identify the location of micro size zones of activity production and consumption the sizes of these zones and the diffusive exchange rate across interfaces e g sediment water interface and biofilm water interface The SensorTrace Profiling program gives you an interactive platform to experiment with activity calculations from these concentration profiles For the interpretation the program uses the shape of a measured concentration profile together with modeling techniques The model implemented in SensorTrace Profiling is based on the method published by Peter Berg and coworkes in 1998 optimized for biogeochemical interpretations of solutes in sediment pore water BACKGROUND A crude amount of information and understanding can be achieved from analyzing the shape of the concentration curve see rules of thumb However going beyond the crude qualitative statement the model used in the Activity tab describes the measured concentration profile by the transport phenomena and processes occurring at different layers within the investigated system and how the concentration in each layer is affected by the transport phenomena and
39. re the profile is stopped Step size um is the vertical step depths by which the sensor is moved from start to end position The step size should not be smaller than the size of the sensor tip e g if the sensor has a tip size of 50 um the step sizes should not be smaller than 50 um Safe The safe position is the position where the motor will rest between each profile The safe position is to ensure that the sensor is resting outside the tissue or sediment between replica profiles In 2D and 3D profiles this is also the height above the sample where the sensor tip will be moved parallel to the sample surface Therefore be sure that the safe position is well above the sample surface Sensor angle This box calculates the actual distance the motor should move a sensor to give the distance added in step size Add the angle in which the motor is penetrating the sensor 38 NOTE You can change the profile settings at any time while the profile is running tip into the sample To be added is the angle the motor has been tilted compared to its vertical position When the motor moves the sensor vertically into the surface the angle 15 0 If the motor is moving the microsensor with an angle of 30 compared to its vertical position and you want the sensor to move 100 in detph you add 30 in to the box and the motor will actually move the sensor 115 um 2100 um cos 30 Wait before measure s When the position
40. s and step sizes are given in um unit Example if you want to define the surface of the study sample as the zero position move the sensor tip to the sample surface using the Move by step keys OR by manually moving the sensor When the sensor approaches the surface or if the sensor is hard to see it is a good idea to use small increments Verify the position with a microscope Enter 0 in the New Depth um field and press 5 Home Subsequently the values in Actual Depth and New Depth um will reflect the re defined depth scale Sometimes if it is not possible to see the sensor it will be necessary to do the first profile find the sample surface from there and redefine the depth scale using the above procedure If subsequently you want to move the sensor to a specified position e g 1000 um above the sediment type 1000 under New Depth um and click GoTo 29 CALIBRATION TAB Calibrations are performed in the Calibration tab The individual NOTE sensor tabs at the top show whether the sensors are calibrated or Profiling is only not Calibrations are stored for 24 hours in the calibration tab possible with a calibrated sensor For information on calibration of a specific sensor consult the sensor manuals Choose the SENSOR you wish to calibrate The sensor name type and calibration unit is shown for each sensor The mV signal in the middle is the current raw sensor signal for the chosen s
41. s and Motors and control the sensor position from here see how under the Sensors and Motors page 27 Z axis X axis Y axis gt O Manual O None O None O Motor O Manual O Manual O Motor Start um Start um B x End um End um 1000 Step size um Step size um 100 Z axis The vertical movement of the z axis is controlled by the Profiling tab X and Y axis The x and y horizontal movements are controlled from this tab The start position of the sensor in the x movement and y movement is defined in START The start position of the sensor is defined compared to its current position Define also END position and STEP SIZE moving in the x or y direction 2D option For the 2D option the program will make one z profile defined in the Profiling tab then move in the x direction and make another z profile and so on The y axis is set on NONE In a 2D setting one cycle includes all the profiles measured along the x axis 35 3D option In a 3D setting one cycle includes all the profiles measured along both the x axis and y axis The program will make one z profile then move in the x direction make another profile and so on When all profiles are performed in the x direction it will move one step in the y direction and repeat When all profiles are made in the x y grid it will return to the safe position and wait for a period of time given by the Delay between s defined in the Profiling tab 36 PROFILING TAB T
42. solutions by clicking and thus highlighting these in the table Furthermore the effect of changing boundary conditions and or minimum zone width can also be tested Save solution Press the SAVE SOLUTION button to save the analysis in the Experimental overview tab under the analyzed profile If the statistics indicate that the number of zones you have chosen in the max zones are too high or too low you can either reduce or increase zone number in the max zone setting Repeat the profile analysis and save the best solution An Analysis can be deleted in the Experiment overview by first right clicking the mouse and then choose DELETE 52 Export data Click the Export button to export data in a Microsoft Excel compatible output file format CSV Choose how to export the data 1 DATA POINTS AND SELECTED ROW IN TABLE where you will save the calibrations comments and for the selected zone number the statistics and the volume base rates of production consumption Choose how to export data O Export Data points and selected row in table O Export entire result table NOTE The application uses as separator A 3 J vu 4 Devices used 3 Instrument name channel Type vat Range sensor name comment 7 Oxymeter S N 6061 A 02 umoll Bipolarsvolt O Sensori 8 MicroOptode Meter 102 umoll BipolarSVolt Os Sensor 9 Micro ptode Meter 202 BipolarsVolt Os Sensor 10 Mi
43. ted sensors The Live Data holds up to the last 24 hours You can change the height of the Live Data window by dragging its upper border Comments calibrations points and other events generated by the user or the program can be seen as colored marks in the Live Data window For further information on comments and events see also the section on the Comments tab 1 140000 E 120009 A 1 100000 M ccc sh EV S z E E m X axis scale The time scale x axis is controlled the SHow LAsT drop down list where a number of time intervals can be chosen To have a look at a certain time span zoom in on this area by dragging a rectangle with the mouse from the upper left corner to the lower right corner of the area of interest To un zoom right click and select Reset ZooM LEVEL or double click directly on the graph Y axis scale By default the y axis auto scales to accommodate the maximum and minimum signals that are shown in the Live Data 24 Graph To have a look at a certain part of the graph zoom in on this area by dragging a rectangle with the mouse from the upper left corner to the lower right corner of the area of interest The mouse wheel can also be used Point the cursor on the part of the graph of interest and zoom to zoom in and out using the wheel Calibrated un calibrated You can control whether the graphs show calibrated signals or raw signals for the sensors by che
44. tric noise in the system Check that the system is properly grounded Connect the Ground connection on your sensor instrument to a ground source a waterpipe or similar Sometimes it can also help to ground the meter directly to your measuring set up with a wire going from the Ground connection of the sensor instrument to the liquid you are measuring in UNISENSE UNISENSE DENMARK www unisense com infogunisense com
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