Techne, TC-3000
Contents
1. To delete a step To insert a new step before the first step use the Up Arrow or Down Arrow keys to select Number cycles Press the Decimal Point key to insert a new step To insert a new step after an existing step use the Up Arrow or Down Arrow I keys to select the step Press the Decimal Point key to insert a new step Number cycles Step 94 02C 00m30 max o insert Step A d to edit lt to delete step Number cycles Step 94 02C 00m30 max DEL TER 4 Yes or V No Use the Up Arrow or Down keys to select the step you wish to delete Press Delete lt to delete the step The prompt line will flash between DEL STEP and YES or NO Press Enter lt to delete the step or Press End V to return to editing the step 27 To add a new stage before an existing one Number cycles step 94 08 00m30 max dto insert stage lt to delete stage To add a new stage at the end Use the Up Arrow or Down Arrow NA keys to highlight the stage number Press Enter A new stage will be added before the existing stage The other stage numbers will be adjusted as appropriate The new stage can now be edited as described above Fin extn 72 0 C 5m00 Fin hold POTOKE to insert stage To delete a stage Use the Up Arrow or Down Arrow NA keys to hig2. YES OR V NO Number cycles 30 Step 94 0 C 00m30 max Hfst 55 02C 00m30 max AV dto edit V to save Use the Up Arrow or Down Arrow keys to move to the step where you want an increment or decrement temperature or time Press Enter lt to go into edit mode Press Pause J I The prompt line will change to flash between CHANGE STEP and YES or NO Press Enter lt The cursor will again move into the edit mode Now press the Up Arrow key The step will change to fst meaning first and the software will insert another line after it entitled Ist meaning last Press Enter lt twice to go into edit mode Number cycles 30 Step 94 0 C 00m30 max fst 65 2c 00m30 max edit temperature Step 94 0 C 00m30 max Est 03 00 00m0 max lst 55 02C 00m30 AV d to edit lt to delete step to insert step Step 94 0 C 00m30 max Fer 65 0 C 00ms0 max lst 55 0 C 1m30 edit time For hrs mins Use the Alpha numeric keys to change the values of the fst line to the first temperatures and or times you require Press Enter d after each field you change You will exit Edit Mode when you reach the end of the line Use the Down Arrow key to move onto the Ist line Press Enter lt to edit the step Use the Alpha numeric keys to change the va
3. 230V Reino Unido 5AMP El fusible una vez instalado protege tanto al equipo como al usuario Astep rese de que los equipos marcados 230V en la placa indicadora funcionan a 220V y de que los equipos marcados 120V funcionan a 110V No obstante en ambos casos la velocidad de calentamiento se ver reducida en un 8 aproximadamente La placa indicadora est situada en la parte posterior del equipo 3 Conecte el cable a la toma de tensi n en la parte posterior del equipo 4 Sit e el aparato en un lugar apropiado tal como una superficie de trabajo plana o si fuera necesario incluso en una campana con extractor de humos astepur ndose de que las entradas de aire en la parte inferior no queden obstruidas 5 Los s mbolos que pueden aparecer junto a las luces indicadoras en el panel frontal del equipo tienen los siguientes significados Indicador de potencia 6 Los s mbolos que se encuentran en o cerca del interruptor de alimentaci n tienen los siguientes significados Interruptor principal encendido O Interruptor principal apagado La tapa calentada sobre el recorte de la temperatura La tapa calentada se cabe con un circuito independiente para protegerlo contra el recalentamiento En el acontecimiento inveros mil de un problema de sobrecalentamiento con la tapa la unidad se cabe con un fusible termal que quite energ a a la placa del calentador si se excede la temperatura m xima Prueba del aislamiento Este equipo se cabe co
4. The Alpha Numeric keys OO The End or Finish key is used to finish editing programs or to exit a program It is also used to exit a sequence of key operations The Up arrow key is used for scrolling up through the lines on the screen lt is also used to increase certain fields when editing The Down arrow key is used for scrolling down through the lines on the screen It is also used to decrease certain fields when editing The Decimal Point key is used when editing numeric fields It is also used as an Insert key during program editing to insert additional steps and other field sets The Delete key is used during program editing to delete steps and stages The Pause key is used during program editing to insert a pause after a stage or to insert increment and decrement times and temperatures It is also used to pause a program when it is running The Enter key is used to enter an operation or the edit mode It is also used to Accept a field or program change The Alpha Numeric keys are used to enter numbers and letters during programming If it is a numeric field then numbers will be inserted if it is an alpha field then letters will be inserted Each key will cycle through the letters shown on the key 0 will insert a space To insert a number press and hold the appropriate key 19 6 Programming the TC 3000 The table below illustrates the symbols whic
5. when you are satisfied with the program you can finish and accept what you have done by first pressing the End V key Then follow the instructions on screen If you are not sure wait for the instructions in the prompt line to scroll back to the first line that you see To run a program Programs Tnformat en Cont lqutat von IV to select Programs Tnfornation Configurar von INV d to select New program 2 STEP TEMPLATE 5013 3 STEP TEMPEATE 5097 Md coe select DAVID DONNA tv d to select DONNA Run Program View Program hv d to select RUN PROGRAM dd Yes or V No When the unit is switched on the display will show this screen If known type in the Speed dial number for the required program and press nn This will take you directly to the required program If you do not know the Speed dial number press Enter lt to access the list of stored programs Next either 1 Scroll down to the required program using the Up Arrow and Down Arrow y keys 2 Or type the first letter of the program name in the New program field this will then list all the program names beginning with that letter Use the Up Arrow and Down Arrow keys yy to select the required program Press Enter to accept lt For example Donna To run the program select Run program and press Enter A screen similar to this o
6. decrease when a cycle is repeated The memory can store up to 80 programs Before using the TC 3000 make sure you have read this manual carefully If there is any doubt relating to the proper use of this equipment the staff at Barloworld Scientific or your supplier will be happy to assist you Unpacking When unpacking the unit check that the following have been removed from the packing e The TC 3000 unit e Mains cable e Guarantee card e The TC 3000 Operator s Manual e The TC 3000 Reference Card Make sure you keep the original packing in case you ever need to return the unit for service or repair Barloworld Scientific accepts no responsibility for damage incurred unless the unit is correctly packed and transported in its original packing Warning A Position the unit so that the mains on off switch is easily accessible and there is a minimum distance all around of 200mm from walls or other items If a safety problem should be encountered switch off at the mains socket and remove the plug from the supply Never move or carry the unit when in use or connected to the mains electricity supply The unit should be carried using both hands Please note that the keypad on the TC 3000 is easily damaged by sharp objects such as pens pencils and fingernails Damage of this sort will be considered as misuse and will invalidate the guarantee on this component Although the unit is designed to cool the samples to 4 C prolonged incub
7. 2 This equipment must be earthed Electromagnetic Compatibility EMC Radiated Emissions and Immunity conform to the limits listed in EN 61326 Electrical equipment for measurement control and laboratory use Note The control specifications are quoted at an ambient temperature of 20 C The specification may deteriorate outside an ambient temperature range of 18 C to 30 C 15 4 User operation and consumables Heated lid To release the heated lid lift the lid catch and the lid will open To close the lid push down gently until the catch clicks in place Do not close the lid without tubes being fitted in the block Tubes or reaction vessels Barloworld Scientific does not recommend any specific tube or reaction vessel other than those described in this manual We recommend using reaction volumes between 20 and 200ul The tubes must withstand a pressure of 1 atmosphere at 100 C Any vessel must be able to withstand the temperatures you are using without any danger of them deforming to the point where they fracture To test your tubes put 25ul of water into each of 5 tubes and subject them to a typical thermal cycling protocol At the end of the program measure the volume remaining using a micropipette A loss of more than 1 or 2ul indicates a vapour leak The amount of volume loss you observe and the change in reactant concentrations you can tolerate determine the minimum volume that can be used Typical volume losses of 1pl in
8. 30 cycles allow the use of samples of 20ul or less During the final cool down a ring of condensation may form above the liquid level but below the top of the sample block This is normal and does not effect the reaction 0 5ml Tubes The heated lid mechanism of the TC 3000 with the 0 5ml block is optimised for flat top tubes it is not suitable for use with domed cap tubes In order to fit the maximum number of tubes in the block they need to be placed so that all the hinges are in the same relative orientation An example is illustrated below Place the tubes in the block in the same orientation in order to achieve the maximum capacity Always make sure the pressure on the heated lid is balanced by spreading the tubes across the block or inserting some dummy tubes if necessary 16 Switching on TC 3000 Version X XX Ele 2532 Zmll or 20x0 5ml Programs Inrormat Lon Contrigusardon Sv to select When you switch on the unit a screen similar to the one shown here will appear briefly This indicates the software version and block type installed in the unit When the initial screen has disappeared the screen shown here will automatically appear There are three options 1 Programs for creating and running programs 2 Information for obtaining information about the unit e g serial number 3 Configuration for setting up program defaults and settings Each of these is explained
9. Enter lt to accept When you have set all the defaults press the Enter lt key to go back to the Configuration menu Note altering the default values does not change existing programs Program defaults Settings Passwords Controller reset 36 Highlight Settings then press Enter lt Set date Set time Date format Auto restart Beeper Coms address Coms speed XX XX XX XOA dmy on on il 4800 PY 4 to select Passwords The complete list of settings is given here Auto restart on will allow the unit to restart from where it left off should there be a power failure and the power returns The Comms address refers to the location of a unit in a daisy chain and must be unique if there are several units linked to each other The optimum Coms speed is set at 4800 When using the Gensoft control software ensure all cyclers are set to the same value Edit any line as described for Program defaults above Press Enter lt to accept the new value When you have finished press the Enter lt key to go back to the Configuration menu Program defaults Settings Passwords Controller reset O DONNA GEORGE It na to select ADMIN PASSWORD Password Confirm d to accept 2 Admin passwd DONNA GEORGE DEL PASSWORD d YES Y NO The Passwords function allows the administrator to change
10. VORSICHTIG UND TRAGEN SIE SCHUTZHANDSCHUHE Legen Sie hei e Gegenst nde NICHT auf oder in die N he von leicht brennbaren Materialien vermeiden Sie Arbeiten in der N he von leicht entz ndbaren Fl ssigkeiten oder Gasen Bringen sie KEINE Fl ssigkeiten direkt in Ihr Ger t Benutzen Sie immer den normalen Menschenverstand Installation 1 Alle Techne Ger te werden mit einem Stromanschlu kabel geliefert Dieses ist entweder fest mit dem Ger t verbunden oder zum Einstecken 2 Vergleichen Sie ob die Spannung Ihrer Stromversorgung mit den Angaben auf dem Typenschild des Ger te bereinstimmen Verbinden Sie das Stromanschlu kabel mit einer geeigneten Stromversorgung gem der n chstehenden Tabelle Achtung Das Ger t mu geerdet sein um die elektrische Sicherheit zu gew hrleisten Verbindungen 220 240V 110 120V Stromf hrend Braun Schwarz Neutral Blau Wei Erde Gr n Gelb Gr n Ger te die f r 230 Volt ausgelegt sind k nnen auch bei 220 Volt arbeiten Ger te f r 120 Volt auch bei 110 Volt In beiden F llen verringert sich die Aufheizrate um ca 8 Das Typenschild befindet sich hinten am Ger t 3 Stecken Sie das Stromkabel in die vorgesehene Buchse hinten am Ger t 4 Stellen Sie das Ger t auf eine ebene Arbeitsfl che bzw falls erforderlich unter einen Laborabzug Beachten Sie da die Entl ftungsrippen an der Ger teunterseite immer frei zug nglich sind 5 Wenn die Anzeigenl mpchen an der Vorderseite
11. computer using an RS 232 cable If you are driving one TC 3000 unit you will need part number FGEN232 which includes a 9 pin female to female null modem RS232 fully screened cable Gensoft on disk and an instruction sheet Connecting several units to the computer using the daisy chain cables If you are driving two or more units then you will need part number FGEN485D a 230V UK power pack set or FGEN485E a 230V European power pack set or FGEN485P a 120 100V USA power pack set power pack set includes the connection to a computer which also converts to RS485 a power pack the necessary cables a termination box and the software on disc with an instruction sheet You will also need one or more of the following 38 FGENTWO A two unit set which includes two connectors for the cyclers and a cable to interconnect them FGENFIVE A five unit set which includes five connectors for the cyclers and four cables to interconnect them FGENTEN A ten unit set which includes ten connectors for the cyclers and nine cables to interconnect them FGENONE A one unit set for extending the daisy chain further which includes one connector for the cycler and one cable for connecting it to the daisy chain Notice from the diagram that the cable connections to the cyclers are alternately bottom to top on the connector Computer Adaptor if necessary Computer connector RS485 converter Connector Terminator Cables Power pack Power cabl
12. fournisseur pour r paration Deutsch Bitte lesen Sie diese Bedienungsanleitung komplett bevor Sie dieses Ger t benutzen Sicherheit des Anwenders Alle Benutzer von Techne Ger ten m ssen Zugang zu der entsprechenden Literatur haben um ihre Sicherheit zu gew hren Es ist wichtig da diese Ger te nur von entsprechend geschultem Personal betrieben werden das die in dieser Gebrauchsanweisung enthaltenen Ma nahmen und allgemeine Sicherheitsbestimmungen und vorkehrungen beachtet Wenn das Ger t anders eingesetzt wird als vom Hersteller empfohlen kann dies die pers nliche Sicherheit des Anwenders beeintr chtigen Die Ger te von Techne entsprechen den internationalen Sicherheitsbestimmungen und sind mit einem automatischen bertemperaturabschalter ausgestattet Bei einigen Modellen ist der bertemperaturabschalter verstellbar und sollte je nach Anwendung entsprechend eingestellt werden Bei allen anderen Modellen ist der Temperaturschutz voreingestellt um Sch den am Ger t zu vermeiden Wenn ein Sicherheitsproblem auftreten sollte mu das Ger t ausgeschaltet und vom Stromnetz getrennt werden Warnung HOHE TEMPERATUREN SIND GEF HRLICH sie k nnen dem Bediener ernsthafte Verletzungen zuf gen und brennbare Materialien k nnen sich leicht entz nden Techne hat bei der Konstruktion dieses Ger tes sehr darauf geachtet da der Bediener vor Gefahren gesch tzt ist Dennoch sollten Sie auf die folgenden Punkte achten e SEIEN SIE
13. la prise murale et enlevez la prise connect e l appareil Avertissement DANGER DE TEMPERATURES ELEVEES les op rateurs peuvent subir de graves br lures et les mat riaux combustibles risquent de prendre feu Techne a apport un soin tout particulier la conception de ces appareils de fa on assurer une protection maximale des op rateurs mais il est recommand aux utilisateurs de porter une attention sp ciale aux points suivants PROCEDER AVEC SOIN ET PORTER DES GANTS POUR SE PROTEGER LES MAINS NE PAS poser d objets chauds sur ou pr s de mat riaux combustibles NE PAS utiliser l appareil proximit de liquides ou de gaz inflammables NE PAS verser de liquide directement dans l appareil Installation 1 Tous les appareils Techne sont livr s avec un c ble d alimentation qui peut tre int gr l appareil ou raccorder 2 Avant de brancher l appareil v rifiez la tension requise indiqu e sur la plaque d identification Raccordez le c ble lectrique la prise appropri e en vous reportant au tableau ci dessous Il est important que l appareil soit reli la terre pour assurer la protection lectrique requise Connexions 220 240 V 110 120 V Phase Marron Noir Neutre Blue Blanc Terre Vert juane Vert Le fusible l int rieur de l appareil est destin assurer la protection de l appareil et de l op rateur Remarque les appareils dont la plaque indique 230 V peuvent fonctionner sur 220 V et ceu
14. leuchten hat dies folgende Bedeutung u Ger t ist eingeschaltet 6 Die Symbole auf oder neben dem EIN AUS Schalter an der Ger ter ckseite bedeuten I An O Aus Die geheizte Kappe ber Temperaturausschnitt Die geheizte Kappe wird mit einem unabh ngigen Stromkreis gepa t um ihn vor der berhitzung zu sch tzen Im unwahrscheinlichen Fall eines bertemperaturproblems mit der Kappe wird die Ma einheit mit einer thermischen Sicherung gepa t die Energie zur Heizung Platte entfernt wenn die maximale Temperatur berstiegen wird Isolierung Pr fung Diese Ausr stung wird mit HF St rung Ausgleichschaltkreis gepa t Jede m gliche berpr fung der elektrischen Isolierung mittels pr fenden des Hochspannungsnichtleiters z B wie in BS EN 61010 1 mu mit nur einer DC Spannung durchgef hrt werden Diese Ma einheit enth lt Halbleiterbestandteile die vorbei besch digt werden k nnen elektrische Feldwirkungen Nach dem Gebrauch Vergessen Sie nicht da Teile des Ger tes die Gef e die Bl cke und andere Zubeh rteile nach dem Erhitzen von Proben noch sehr hei sein k nnen Bitte beachten Sie die oben genannten Vorsichtsma nahmen Garantie Die Garantiedauer des Ger tes ist auf der beiliegenden Garantiekarte angegeben und schlieRt Fehler im Material oder der Verarbeitung ein Die Garantiedauer beginnt am Tag des Einkaufs Samtliche defekte Teile werden innerhalb dieses Zeitraumes kostenlos ersetzt unter der Vorauss
15. max step 60 c 0m30 Has step TL NE Om30 max 5 step 120 Om30 max Final Extn 729 5m Stage 2 Final hold 4 C 10m an 20 step 94 c Om30 max step 55 c Om30 max step 712 0m30 max Final Extn 72 c 5m Final hold 4 C 10m Programming Create a new program by selecting x DE Eee Et New Program from the menu and press 2 STEP TEMPLATE 5013 Enter d 3 STEP TEMPLATE 75027 MN to select Give the program a name and edit any of the UNNAMED default fields as described above Heated lid 105 C Preheat lid on A Vv to edit V to save To add a stage and then edit it Use the Down Arrow key to scroll to the o In denat 94 0 C 5m00 dotted line below Hot RA it will start to Hol store Grr flash Press Enter lt to insert a stage to insert stage 26 Number cycles step 94 0 C 00m30 max l A edit no cycles Number cycles Step 94 02C 00m30 max a to IAsert step A d to edit lt to delete step To insert additional steps A new stage will be inserted which consists of a Number cycles field and one step Edit the number of cycles by typing the number required then press Enter Use the Down Arrow Y key to move to the step Press Enter lt to edit the step as described above Press Enter lt again to accept Number cycles Step 94 0 C 00m30 max a7 o insert SESH A d to edit
16. select Run program Copy Program View program Press the Enter key lt to accept it Copy program IV to select 21 UNNAMED Heated Lid LOSC Preheat Lid on PV dre ea To give a program a name A new name can now be given to the copied program see below E UNNAMED Heated Lid LOSC Preheat Lid on PV 72 to edie 4 OOo DONNA Heated Lid OSC Preheat Lid on Ay d to edit V to save To save the program To type in a name for the program press Enter lt and a cursor will appear in the position shown Using the keypad type in the name you wish to give the program The letters will scroll round with successive presses for example the 2 key will give A with one press B with two presses C with three presses and 2 with four presses Five presses will again give A When the name is complete press Enter There are now two options 1 Save the program and run it as it is or 2 Edit the program then save and run it DONNA Heated Lid ASES Preheat Lid on SAVE 4 YES or V No DONNA Run Program View Program IV to select 22 A program can only be run once it has been saved Press the End V key The prompt line will flash between SAVE and YES or NO Press Enter lt for Yes to save the changes you have made If you press End W for No then all the changes yo
17. E QUE PAR DU PERSONNEL QUALIFIE LORSQUE LES PANNEAUX AVANT ARRIERE ET LATERAUX SONT DEMONTES L OPERATEUR EST EXPOSE A DES TENSIONS QUI PEUVENT ETRE MORTELLES CET APPAREIL NE CONTIENT AUCUN ELEMENT QUI DEMANDE UN ENTRETIEN DE LA PART DE L UTILISATEUR Dans le cas peu probable o votre appareil pr sente un d faut de fonctionnement auquel il est difficile de rem dier il est alors pr f rable de contacter votre fournisseur et le cas ch ant de renvoyer le mat riel Veuillez inclure une description d taill e du probl me constat et retourner l appareil dans son emballage d origine Techne ne sera pas tenu responsable des dommages subis par tout appareil dont l emballage est inad quat pour le transport Pour plus de s ret contactez votre fournisseur Voir le certificat de d contamination livr avec le produit 1 Nettoyage Avant de nettoyer l appareil assurez vous TOUJOURS que le c ble d alimentation est d connect et laissez la temp rature redescendre en dessous de 50 C Utilisez un chiffon impr gn d eau savonneuse pour nettoyer l appareil Veillez ne pas introduire d eau dans l appareil N utilisez pas de produits abrasifs 2 Fusibles La protection de l appareil est assur e par un ou deux fusibles dont le remplacement ne peut tre effectu que par un personnel qualifi Si les fusibles sautent sans arr t il s agit d un probl me s rieux Nous vous conseillons dans ce cas de prendre contact avec votre
18. TC 3000 Operator s Manual amp Barloworld Scientific v1 0 Contents A o a A iii 5 Unpacking eera 222322222 pelado pol per poc ne non mr a 5 WIaTBING ze a ne aaa o a ee een 5 2 Safety and InstallatlON cocinero 8er etereHeHteroreHenrbre 6 o RP Re RAR RE 6 FRANCAIS enr inertie ARALAR AAA EA 7 DOES Os ada ae el En E E E E 9 ESPINO Ne ut 10 3 TC 3000 SpecifICatlon cortesana 14 4 User operation and consumables oooooooocccccccccccccccccoocononoccnncnncnnnannnn ono ncnnnnnnns 16 O A RN 16 Tubes dr reactonVvessels sise ds dE NS tn cat eer 16 OSM TUBOS 4A aati date state A ee 16 SWITCH Oc tai dd de edo db ds tt nt dde dd es tan ee 17 5 Front panel controls 18 TReLCO DIS EEE ce o o COLS 18 INOICALOES Se A A AO 18 A ona Ran 19 6 Programming the TC 3000 oooooccoccccococoococcccccccccconananoncnnnncnnnnnnnnnnnnnncnnnnnnnnnnnnns 20 Programming Options EE siecle recense ne eE EEn ent a ententes ee 21 Option 1 Creating programs using templates 21 Option 2 Creating a new program uuuneenennnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn nn 25 Example DOME Heilen ELDER EEE EEE 26 Programming EBD A 26 To program increment or decrement temperature or time 29 Torun a PODA E ee ee ee een 33 E INTO MATO A A AAA ATA sense nenne 35 Ge COMMUTATION era ale 35 9 Running the TC 3000 from a PC 38 Connecting one unit to the computer using an RS 232 cable 38 Connecting several units
19. a com www techneusa com Italy Barloworld Scientific Italia Srl Via Alcide de Gasperi 56 20077 Riozzo di Cerro al Lambro Milano Italia Tel 39 0 2 98230679 Fax 39 0 2 98230211 e mail marketing barloworld scientific it www barloworld scientific it Germany Techne AG Obere Hauptstrasse 67b D 09235 Burkhardtsdorf Germany Tel 49 0 3721 271888 Fax 49 0 3721 270608 e mail info techne de 13 3 TC 3000 specification Temperature Temperature range Temperature set point precision Block uniformity over full range Temperature accuracy End of program cooling below ambient 4 C to 99 C 0 1 C 0 5 C 0 5 C Yes Heating Cooling Rate Heating rate between 55 C and 90 C on a 50 C to 95 C step Heated Lid 25 x 0 2 ml micro tubes 20 x 0 5 ml micro tubes 3 6 C sec 3 6 C sec Cooling rate between 90 C and 55 C on a 95 C to 50 C step 25 x 0 2 ml micro tubes 20 x 0 5 ml micro tubes Heated lid enable disable Selectable heated lid temperature Over temperature cut out Temperature sensor Heater type Heater power Warm up time 2 0 C sec 1 8 C sec Yes 100 C to 115 C Fixed at 145 C Thermistor Etched foil 33W 4 min approximately ambient to 105 C The heated lid will only come on if the set temperature is above 35 C Programming Program naming using alpha keys Program password protection Number of programs 3 step Maximum number o
20. ations at this temperature may reduce the life of the cooling elements in the block Where possible it is advised to use an end of run hold temperature closer to ambient e g 10 C to 15 C in order to prolong the life of the cooling elements L Aspects of the PCR process are claimed in USA Patent Nos 4 683 195 4 683 202 and 4 965 188 Use of the TC 3000 in such processes does not convey a licence to practice the processes themselves 2 Safety and installation English Please read all the information in this manual before using the unit Operator Safety All users of Techne equipment must have available the relevant literature needed to ensure their safety It is important that only suitably trained personnel operate this equipment in accordance with the instructions contained in this manual and with general safety standards and procedures If the equipment is used in a manner not specified by Barloworld Scientific the protection provided by the equipment to the user may be impaired All Techne units have been designed to conform to international safety requirements and are fitted with an over temperature cut out On some models the cut out is adjustable and should be set to suit the application On all other models the cut out is preset to protect the unit If a safety problem should be encountered switch off at the mains socket and remove the plug from the supply Warning HIGH TEMPERATURES ARE DANGEROUS they can cause ser
21. cification is 3 6 C second yet can only manually set it to 2 0 C second Why is this A5 If you set the ramp rate to max the block will heat at 3 6 C second the unit sets its own control parameters If you program a ramp rate the control parameters reduce the ramp rate This is a feature of the software and not the actual ramp rate If you require further assistance please contact us at techne barloworld scientific com We are continually striving to improve our thermal cyclers and software If you have any comments and suggestions on how we can do things better please send them to us at techne barloworld scientific com 42 13 Additional information Brief fault finding notes and a list of replacement parts are given in this section Note that this equipment should only be dismantled by properly trained personnel Removing the outer cover exposes potentially lethal mains voltages There are no user serviceable parts within this equipment Fault Finding Should you have any problems with your TC 3000 which cannot be easily remedied you should contact your supplier and return the unit if necessary Please include details of the fault observed and remember to return the unit in its original packing Barloworld Scientific accept no responsibility for damage to units which are not properly packed for shipping if in doubt contact your supplier giving the full serial number of the unit and software version number shown when the unit i
22. ct from the power supply and allow it to cool to below 50 C Remove the four screws on the underside of the unit and drop the block fan assembly down Unplug the assembly from the wiring and remove the assembly from the unit Refitting the block fan assembly is the reverse procedure be careful of the wires as you refit the block S A1DD00000 000 Underside of the TC 3000 Four screws which hold the block fan assembly in place The outer case of the TC 3000 can be cleaned with a cloth dipped in water or ethanol methanol or formaldehyde can also be used No part of the case or cover should be immersed in the solvents Do not use aggressive solvents such as acetone or abrasive cleaners Before using any cleaning or decontamination method except those recommended here the responsible person should check with Barloworld Scientific that the proposed method will not damage the equipment 41 12 Some frequently asked questions Q1 What is the Pause function at the start of the program used for A1 Some users prefer to preheat the heated lid before placing the samples into the unit The pause feature is used to stop the unit after the 4 minute heated lid preheat step It will also sound an audible alarm indicating that the machine is ready for the sample tubes or plate to be added Pressing the Pause key followed by the Enter key will commence the remaining program Q2 Why do you have to press some keys twice to mak
23. d V key by mistake and you want the program to continue press End Y again When a program is stopped the unit will emit a beeping sound and the original program screen will appear Program Information DONNA Heated Lid on SEabus ramping d to exit 7 Information Programs Information Conrigurak von tv d to select Block 20x00 Sm Block s n 139160 2 Unit s n GPO000 01 S w version LA Zz H w version 01 Comms address al Unit cycles 624 Block cycles 69 8 Configuration Pressing Enter lt while a program is running will give information about the current run including which program is actually running and the unit status e g holding or ramping An example is shown here The Information field on the front screen contains all the unit specific information such as serial number and software version Press Enter lt to access the unit information The complete list is displayed here Note that the Comms address must be unique for each unit if more than one cycler is linked to a PC using the Gensoft control software Warning The Configuration software should only be accessed by designated personnel as some parameters can be changed or may be lost when using it Programs Intormar eon Configuration IV to select CONFIGURATION Password pad RO ao ae pl V to exit The Configuration field on the front screen allows a system administrator access to s
24. e Upgrading the software in your TC 3000 Connect your TC 3000 to your computer using an RS232 lead Log onto the Techne web site at www techne com Click on Thermal Cycler Software Update at the top of the page and you will find information on how to upgrade your software Click on Update Software and follow the instructions on the page to install the programs INDUSTRIAL CALIBRATION FLuipiseo BATHS 39 10 Notes on transferring protocols from other thermal cyclers The TC 3000 heats at over 3 6 C s and provides the highest levels of temperature uniformity Many slower machines have historically begun the countdown on hold times before the set temperature is reached in order to avoid any overshooting in sample temperature The slower the heating rate the more significant the loss in hold time i e if a 30 second hold time is set at 55 C then perhaps 5 to 10s will be lost in between the start of the time countdown and actually reaching the set temperature The overall effect of this method of control is that your sample if cooling from a higher temperature is subjected to an environment with an elevated temperature compared to the target temperature for a significant part of the hold time The TC 3000 provides the user with what you program is what you get Due to the rapid and responsive nature of the Peltier heating system the time between the timer countdown initiating and actually reaching the set temperat
25. e TC 3000 Four screws which hold the block fan assembly in place 43 Accessories The following accessories can be obtained from Barloworld Scientific or your local Techne dealer Item No FTC3 02 B FTC3 05 B FTUBO2TW FTUBO5TW Description 0 2ml Tube Block for TC 3000 0 5ml Tube Block for TC 3000 0 2ml Micro tubes 0 5ml Micro tubes thin wall Replacement parts The following replacement parts can be obtained from Barloworld Scientific or your dealer Item No FCABRTUK FCABRTEU FCABRTUS 6501231 6500129 Description Mains cable and plug 230V UK Mains cable and Schuko plug 230V Europe Mains cable and 3 pin plug 100V 120V US type Fuse T2 5A 230V units Fuse T4A 120 100V units Quantity 1 1 Pack of 1000 Pack of 1000 Quantity required 1 1 1 2 2 Should the mains lead need replacement a cable of 1mm of harmonised code HOVV F connected to an IEC320 plug should be used IF IN DOUBT CONSULT A QUALIFIED ELECTRICIAN 44
26. e the unit do what you want it to do A2 With many of the program features there is a fail safe function which asks if you are sure you want to perform a particular action You are required to press one key to select a function followed by a second to accept it Q3 What is Hot Start A3 A hot start is used for reducing non specific product formation during the first cycle of amplification The Hot Start programming step is used to pause the instrument at a specific temperature typically around 70 C after the initial template denaturation The reason is to allow the manual addition of unmodified Taq DNA polymerase which may loose activity if added during the initial 5min denaturation Heat activated Taq or Hot Start enzymes do not require this step Q4 What is the incremental function See pages 29 31 A4 Incremental timing and temperature are used to increase or decrease either the time or temperature incrementally over the number of cycles in a stage Incrementation of extension time is used with Long PCR which is when large template fragments are to be amplified e g 27kb lambda DNA 40kb genomic DNA Decremental temperature is used for protocols such as Touchdown PCR where one starts with a high annealing temperature in the first cycle and gradually decreases the temperature over the number of cycles in the stage This ensures that only the specific product is amplified Q5 The heat up ramp rate in the spe
27. ee lines at a time Use the Down Arrow and or the Up Arrow keys A y to select the option you require Press the Enter key lt to select the option Select Edit program and a screen similar to this will appear Use the Down arrow and or the Up arrow keys y to select the field or step you want to edit You can now edit the active field or step by first pressing the Enter key 23 DONNA Heated Lid Mio5ec Preheat Lid on edit temperature lt to disable DONNA Heated Lid HOS SC Preheat Lid Bon Ay to change turn on off In denat Ek o0 c 00m30 edit temperature lt to disable In denat 94 02C Pom30 edit time for hrs mins Fin extn 72 0 C 5m00 Fin hold 10 02C tv to edit V to save 24 To edit a temperature type in the required temperature In the example here the heated lid temperature is being edited Press Enter lt to accept Use the Down Arrow and or the Up Arrow keys y to select the next field or step you wish to edit To edit a field with on off settings first select the field and press Enter Use the Up Arrow and Down Arrow keys to toggle between the settings Press Enter lt to accept To edit a step with both a temperature and a time first press Enter lt and type in the new temperature Press Enter again Now t
28. et up all the default settings for the programs time and date settings and to change and remove passwords Access to the Configuration set up is password protected To enter configuration use the alpha keys to type in pad Press Enter lt 35 Program defaults Settings Passwords Controller reset Program defaults There are a number of options which can be changed these are listed here Use the Up Arrow and Down Arrow v keys to select then press Enter 4 to access any of the options Each option is discussed in detail below Program defaults Settings Passwords Controller reset Heated lid 1052C Preheat lid OE Pause OE IE In denat 94 0 C 5m00 HOT erant JE IE IZ OPC Sm00 ORO Fin extn Fin hold Ay to edit Settings Highlight Program defaults then press Enter The complete list of program defaults is given here These are the values that all new programs will take when you start to create a new program To edit any line use the Up Arrow or Down Arrow keys v to select the line you wish to change Press Enter lt then change the value using the numerical keys Press Enter lt l to accept the new value To edit a field with on off settings first select the field and press Enter d Use the Up Arrow and Down Arrow A keys to toggle between the settings Press
29. etzung da dem Defekt keine unsachgem e Handhabung Fahrl ssigkeit oder ein Unfall zugrundeliegt Der unter diese Garantie fallende Service wird vom Lieferanten geleistet Ungeachtet der in dieser Gebrauchsanweisung enthaltenen Beschreibungen und Spezifikationen beh lt sich Techne hiermit das Recht vor nderungen an den Ger ten bzw an einzelnen Ger teteilen durchzuf hren Diese Gebrauchsanleitung wurde ausschlie lich dazu erstellt um Kunden die Handhabung der Techne Ger te zu erleichtern Nichts in dieser Gebrauchsanleitung darf als Garantie Bedingung oder Voraussetzung verstanden werden sei es die Beschreibung Marktg ngigkeit Zweckdienlichkeit oder sonstiges bez glich der Ger te oder deren Bestandteile Wartung durch den Bediener BEACHTEN SIE DASS DIESES GER T NUR VON TECHNISCHEN FACHKR FTEN GE FFNET UND DEMONTIERT WERDEN DARF DURCH ENTFERNEN DES GEH USES ODER GEH USETEILEN SIND BAUTEILE MIT LEBENGEF HRLICHEN SPANNUNGEN FREI ZUG NGLICH IM INNERN DES GER TES BEFINDEN SICH KEINE TEILE DIE VOM ANWENDER GEWARTET WERDEN M SSEN Falls Ihr Ger t nicht ordnungsgem arbeitet wenden Sie sich an Ihren Lieferanten oder senden Sie das Ger t wenn n tig zur ck F gen Sie eine genaue Beschreibung des Defektes bei Verpacken Sie das Ger t m glichst im Originalkarton Bitte beachten Sie da Techne keine Haftung bei Transportsch den aufgrund unzureichender Verpackung bernnehmen Setzen Sie sich im Zweifelsfall mit Ih
30. f steps per stage Maximum number of stages per program Minimum hold time Maximum hold time Incremental decremental temperature Incremental decremental step hold time Programmable ramp rate heat or cool Programmable ramp rate resolution Yes Yes 80 35 25 1 sec 18 hrs Yes Yes Variable in 0 1 C sec steps 0 1 C Running Programs Pause button Stop button End of program alarm Auto restart on power fail Interconnectivity 14 Connection to PC control program Software updates from Techne web site Yes Yes Yes Yes Yes Yes Dimensions Serial Port Power Height 190mm Width 170mm Length 330mm This socket is provided for RS232 PC link to run Gensoft the PC control software Power consumption 230W The units are voltage selectable by a slide switch When the unit is set to 115V it will work at any voltage between 110V and 130V When the unit is set to 230V it will work at any voltage between 198V and 252V Units marked 100V will work at any voltage between 90 and 110V The performance will vary and will not necessarily meet the above typical specification at the extremes of voltage Working conditions The TC 3000 is designed to operate under the following conditions For indoor use only Ambient temperature range 5 C to 40 C Altitude To 2000m Relative humidity Not exceeding 95 Mains supply fluctuations Not exceeding 10 Over voltage category Il IEC 60364 4 443 Pollution degree
31. h appear on the LCD display and the relevant keypad button to which they refer V J t al In the prompt line is equivalent to the End key O On a program line means the program is locked it can be copied and then edited but not edited directly Up arrow A Down arrow Enter o Pause A The following terms are used when programming the TC 3000 Stage Cycle Step Programs A Stage is the name given to the main repeated part of a program containing the Steps to be repeated A program can contain a maximum of 25 stages although typically only one or two are used A Cycle is the number of repetitions of a Stage A Step is a user defined program line containing a temperature a hold time and ramp rate Typically three steps are created within one Stage To enter the programming section of the TC 3000 software highlight Programs using the Information Up Arrow or Down Arrow keys N Const ion NY nd te select New program Press Enter lt This screen lists all the programs saved on the unit Use the Up Arrow and Down Arrow keys 2 STEP TEMPLATE 5014 to scroll through the list A y 3 STEP TEMPLATE 5023 a ER un ram name Kran tovselect DUMDPENEI to Me progra s the 20 unique Speed dial number allocated to the program for rapid retrieval see page 33 Programming Options There are two opti
32. he program or End V to return to the program screen If you want to password protect the program so that other users cannot edit it select Set password A screen similar to this will appear Type in the password you wish to give it and press Enter lt The program list will then display the key symbol next to the name to show that it is locked Other users will still be able to copy view your program but not edit it A second option is to create a completely new program without using templates UNNAMED Heated lid WOSHE Preheat lid Or E Pause Off In denat GA OPC Sm00 AGE Stealzic OE ie 121006 OO 10 02C Pin EXTA alia AOLE A number of typical values are pre entered including Heated lid temperature Preheat lid Initial denaturation Hot start Final extension Final hold These values can be edited and the additional stages and steps required for cycling can be added by following the instructions detailed on the following pages 25 Example programs A couple of example thermal cycling programs are shown below Typical 3 Step PCR 3 Step PCR with multiple Stages Heated Lid 105 Heated Lid 105 Preheat Lid on Preheat Lid on Pause off Pause off In denat 94 c 5min In denat 94 5min Hot Start off Hot Start off Stage 1 Stage 1 umber Cycles 30 Number Cycles 10 Step 94 C Om30 max step 94 c 0m30 max Step SI O Om30
33. hlight the dotted line below the last stage Press Enter A new stage will be added with the next consecutive number The new stage can now be edited as described above Number cycles Step 94 0 C 00m30 max to insert stage lt to delete stage Number cycles Step 94 0 C 00m30 max DEL STAGE A YES or V No 28 Use the Up Arrow or Down Arrow keys to highlight the stage you wish to delete Press Delete lt The prompt line will flash between DEL STAGE and YES or NO Press Enter lt to delete the stage To insert a pause into a program To insert a pause after an existing stage Step 94 0 C 00m30 max follow the steps for inserting a stage as Stage above Number cycles Highlight the Number cycles line then press Step 94 0 C 00m30 max Pause to insert step A Vv d to edit The prompt line will flash between CHANGE Step 94 0 C 00m30 max TO PAUSE and YES or NO Number cycles Press Enter lt to insert a pause Step 94 0 C 00m30 max CHANGE TO PAUSE lt Yes or V No The stage number will then be highlighted with Pause as the only line d to insert step lt to delete step To program increment or decrement temperature or time Under normal circumstances the Hold Temperature of all steps is constant However it is possible to automatically increment or dec
34. in detail in section 6 17 5 Front panel controls Operation or Program lines Prompt line Programs Information Configuration IA Vv to select Decimal Point 8 Insert Up Delete End Pause Down Status Power Enter Alpha Numeric keys The LCD Display The TC 3000 has a four line LCD display Programs The top three lines are information or Information program lines while the bottom line is a Configuration INV to select prompt line The active line flashes on screen and is shown in bold in this manual The bottom line may change or flash between two or three different prompts Number cycles depending upon which program line is active An example of a prompt line which flashes o Step 94 02C 00m30 max between three different prompts is shown ST to insert step here A Vv to edit lt to delete step Indicators O Power This indicator is lit when there is power to the TC 3000 Status This indicator is lit when the TC 3000 is running a program It is off when the unit is stopped It will flash and beep slowly when the unit is paused It will flash 1O after a five second quick sequence of beeps when the unit reaches its final hold temperature 18 The keys The End Finish or Exit key The Up arrow key The Down arrow key The Decimal Point and Insert key S The Delete key D The Pause key B The Enter or Accept key
35. ious burns to operators and ignite combustible material Barloworld Scientific have taken great care in the design of this unit to protect operators from hazards but users should pay attention to the following points e USE CARE AND WEAR PROTECTIVE GLOVES TO PROTECT HANDS DO NOT put hot objects on or near combustible material DO NOT operate the unit close to inflammable liquids or gases DO NOT place any liquid directly in your unit Installation 1 All Techne units are supplied with a power cable This may be integral or plug in 2 Before connecting the mains supply check the voltage against the rating plate Connect the mains cable to a suitable plug according to the table below Note that the unit must be earthed to ensure proper electrical safety Connections 220 240V 110 120V Live Brown Black Neutral Blue White Earth Green yellow Green The fused plug supplied with the mains lead for use in the UK is fitted with the following value fuse to protect the cable 230V UK 10 AMP The fuse in the unit protects the unit and the operator Note that units marked 230V on the rating plate work at 220V units marked 120V work at 110V In both cases however the heating rate will degrade by approximately 8 The rating plate is on the rear of the unit 3 Plug the mains cable into the socket on the rear of the unit 4 Place the unit on a suitable bench or flat workspace or in a fume cupboard if required ensuring that the air in
36. ith a damp soapy cloth Care should be exercised to prevent water from running inside the unit Do not use abrasive cleaners 2 Fuses Your unit is protected by one or two fuses These should only be changed by suitably qualified personnel If the fuses blow persistently a serious fault is indicated and you may need to return the unit to your supplier for repair Francais Veuillez lire attentivement toutes les instructions de ce document avant d utiliser l appareil S curit de l op rateur Tous les utilisateurs de produits Techne doivent avoir pris connaissance des manuels et instructions n cessaires la garantie de leur s curit Important cet appareil doit imp rativement tre manipul par un personnel qualifi et utilis selon les instructions donn es dans ce document en accord avec les normes et proc dures de s curit g n rales Dans le cas o cet appareil ne serait pas utilis selon les consignes pr cis es par Techne la protection pour l utilisateur ne serait alors plus garantie Tous les appareils Techne sont con us pour r pondre aux normes de s curit internationales et sont dot s d un coupe circuit en cas d exc s de temp rature Sur certains mod les ce coupe circuit est r glable pour s adapter l application d sir e Sur d autres mod les il est pr r gl e en usine pour assurer la protection de l appareil Dans le cas d un probl me de s curit coupez l alimentation lectrique au niveau de
37. let vents on the underside are free from obstruction 5 Note that the following symbol next to the indicator lamp on the front panel of the units and has the following meaning the power indicator 6 Symbols on or near the power switch of the unit have the following meanings mains switch On O mains switch Off The heated lid over temperature cut out The heated lid is fitted with an independent circuit to protect it from overheating In the unlikely event of an over temperature problem with the lid the unit is fitted with a thermal fuse which will remove power to the heater plate should the maximum temperature be exceeded Insulation testing This equipment is fitted with RFI suppression circuitry Any check of the electrical insulation by means of high voltage dielectric testing for example as in BS EN 61010 1 must be carried out using only a DC voltage This unit contains semiconductor components which may be damaged by electric field effects After use When you have finished heating samples remember that parts of the unit the tubes block and associated accessories may be very hot Take the precautions listed earlier Guarantee The unit is guaranteed against any defect in material or workmanship for the period specified on the enclosed guarantee card This period is from the date of purchase and within this period all defective parts will be replaced free of charge provided that the defect is not the resul
38. lues of the Ist line to the final temperatures and or times you require Press Enter lt for each field you change You will exit Edit Mode when you reach the end of the line When you run the program it will automatically increment or decrement in equal steps from the first time and or temperature to the last over the number of cycles in the stage For example if the first temperature is 65 C and the last temperature is 55 C over 30 cycles the temperature will decrease 0 33 C each cycle Similarly in the example shown the time would increase from 30 seconds to 1 minute 30 seconds in 2 second increments 31 To save the program Number cycles Step 94 0 C 00m30 max SAVE dd Yes or V No GEORGE Run Program View Program AV d to select Stopping what you are doing A program can only be run once it has been saved Press the End V key The prompt line will flash between SAVE and YES or NO Press Enter d for Yes to save the changes you have made If you press End V for No then all the changes you have made will be lost The program is now ready to run and will be allocated with a unique Speed dial number which can be used to rapidly locate the program from the main start up screen Use the Up Arrow and Down Arrow v keys to scroll through the list of options 32 At any time during programming the TC 3000
39. mente capacitado Si los fusibles se fundieran repetidamente esto indicar a una aver a grave y puede que tuviera que devolverle el aparato a su proveedor para su reparaci n Before switching on Ensure that the voltage selector switch is set to the appropriate voltage for your supply On Off switch Ensure that the appropriate fuses are fitted for your supply or 230V set the voltage selector switch set to 230V and fit T2 5A fuses For 115V set the switch to 115V and fit T4A fuses For 100V the switch will be set to 115V T4A fuses will be fitted Contact Information For technical sales or servicing information contact your local Techne dealer or UK and Rest of World Barloworld Scientific Ltd Beacon Road Stone Staffordshire ST15 OSA Tel 44 0 1785 812121 Fax 44 0 1785 813748 e mail techne barloworld scientific com www barloworld scientific com France Barloworld Scientific France SAS ZI du Rocher Vert BP 79 77793 Nemours Cedex France Tel 33 1 64 45 13 13 Fax 33 1 64 45 13 00 e mail bibby bibby sterilin fr www barloworld scientific com Middle East Techne Middle East Ltd PO Box 27887 Engomi 2433 Nicosia Cyprus Tel 357 22 660427 Fax 357 22 660356 e mail sales techneme com North and South America Barloworld Scientific US Ltd Techne 3 Terri Lane Suite 10 Burlington N J 08016 USA Tel 800 225 9243 Fax 609 589 2571 e mail sales techneus
40. n el trazado de circuito de la supresi n de la IRF Cualquier cheque del aislamiento el ctrico por medio del diel ctrico de alto voltaje que prueba por ejemplo como BS EN 61010 1 se debe realizar usando solamente un voltaje de DC Esta unidad contiene los componentes del semiconductor que se pueden da ar cerca efectos de campo el ctricos Despu s de su uso Cuando haya finalizado el calentamiento de muestras recuerde que las piezas del equipo tales como tubos bloques y dem s accesorios pueden estar muy calientes Tome las precauciones mencionadas anteriormente Garantia Este aparato est garantizado contra cualquier defecto material o de fabricaci n durante el periodo especificado en la tarjeta de garant a adjunta Este plazo inicia a partir de la fecha de compra y dentro de este periodo todas las piezas defectuosas ser n reemplazadas gratuitamente siempre que el defecto no sea resultado de un uso incorrecto accidente o negligencia Mientras se encuentre bajo garant a las revisiones las debe llevar a cabo el proveedor A pesar de la descripci n y las especificaciones de los aparatos contenidas en el Manual del Usuario Techne se reserva por medio de este documento el derecho a efectuar los cambios que estime oportunos tanto en los aparatos como en cualquier componente de los mismos Este manual ha sido preparado exclusivamente para los clientes de Techne y nada de lo especificado en este folleto de instrucciones se tomar como
41. ne will appear Press Enter lt to start the run 33 Cycle 4 30 56m08 Sample 58 0 C Hold 58 0 C 00m17 During the run the LCD screen will report 1 The cycle number 2 The time remaining counting down in seconds 3 The sample temperature whether V to STOP holding or ramping to PAUSE 4 The current step of the run d for info 5 A count down of the hold time An example is shown here Manual pause Cycle 24 30 19m26 Sample 72 0 C Hold 72 0 C 00m10 PAUSE lt lt YES or V NO To pause a program while it is running press Pause I I The run will continue but the prompt line will alternate PAUSE and YES or NO To confirm that you want the program to pause press Enter If you have pressed the Pause key by mistake and you want the program to continue press End V The unit will emit a beeping sound while Sample 72 0 C To resume the run press Pause I Paused followed by Enter to continue V to STOP to CONTINUE lt for info Manual Stop Cycle 28 30 10m39 Sample 94 0 C Hold 94 0 C 00m10 STOP PROGRAM 4 YES or V NO 34 To stop a program while it is running press End V The run will continue but the prompt line will alternate STOP PROGRAM and YES or NO To confirm that you want to stop the program press Enter ak If you have pressed the En
42. nexi n es variable lo que le permite elegir la temperatura step n sus necesidades En otros el sistema de desconexi n viene ya ajustado para evitar da os en el equipo En caso de que surgiera un problema de stepuridad desconecte el equipo de la red Aviso LAS TEMPERATURAS ELEVADAS SON PELIGROSAS pueden causarle graves quemaduras y provocar fuego en materiales combustibles Techne ha puesto gran cuidado en el dise o de estos aparatos para proteger al usuario de cualquier peligro a n asi se deber prestar atenci n a los siguientes puntos e EXTREME LAS PRECAUCIONES Y UTILICE GUANTES PARA PROTEGERSE LAS MANOS NO coloque objetos calientes encima o cerca de objetos combustibles NO maneje el aparato cerca de l quidos inflamables o gases NO introduzca ning n l quido directamente en el aparato Instalaci n 1 Todos los aparatos Techne se suministran con un cable de alimentaci n Puede ser fijo o independiente del aparato 2 Antes de conectarlo compruebe que el voltaje corresponde al de la placa indicadora Conecte el cable de alimentaci n a un enchufe adecuado step n la tabla expuesta a continuaci n El equipo debe estar conectado a tierra para garantizar la stepuridad el ctrica 10 Conexiones 220 240V 110 120V Linea Marr n Negro Neutro Azul Blanco Tierra Verde amarillo Verde El enchufe suministrado con el cable de alimentaci n viene equipado con un fusible del siguiente valor para proteger el cable
43. nit contient les composants de semi conducteur qui peuvent tre nuis pr s effets de champ lectriques Apr s utilisation Lorsque vous avez fini de chauffer les chantillons n oubliez pas que certaines parties de l appareil les prouvettes leurs supports et autres accessoires risquent d tre tr s chaudes Il est donc recommand de toujours prendre les pr cautions cit es plus haut Garantie L appareil est garanti contre tout d faut ou vice de fabrication pour la dur e figurant sur la carte de garantie compter de la date d achat de l appareil Au cours de cette p riode toutes les pi ces d fectueuses seront remplac es gratuitement dans la mesure o la d faillance n est pas due une mauvaise utilisation un accident ou une n gligence Toute r paration sous garantie sera effectu e par le fournisseur Malgr la description et les sp cifications de l appareil donn es dans le manuel de l utilisateur se r serve le droit d effectuer les changements n cessaires l appareil ou tout l ment qui entre dans sa composition Ce manuel a t exclusivement r dig l attention des clients de Techne et aucun l ment de ce guide d instructions ne peut tre utilis comme garantie condition ou repr sentation concernant la description commercialisation adaptation aux conditions d utilisation ou autre des appareils ou de leurs composants Entretien utilisateur IMPORTANT CET APPAREIL NE PEUT ETRE DEMONT
44. ons available for creating programs on the TC 3000 1 Copy and edit one of the pre written templates 2 Create a new program from the defaults Option 1 Creating programs using templates There are two pre written basic PCR templates a two step and a three step template These can be copied and modified as required 2 STEP TEMPLATE Heated Lid 105 3 STEP TEMPLATE Heated Lid MOSS Preheat Lid on Pause HL AE Preheat Lid on Pause GR In denat 940 C Sime BOTE Sees GT In denat SAONE SnO Hot Start ORE Stage 1 Number cycles 30 Seage i Number cycles 30 94 0 C 9215 OPE IZ ADAC Om30 max Om30 max Om30 max Step 94 0 C Om30 max Step 68 0 C 1m00 max SESS SISO SEEP aime lisacia 12 0 End kama lao Le 10 09 tia 72 008 SO hold ORO Final Final Other templates available include Ice bucket incubation at 4 C Ligation incubation at 15 C and an RT PCR template Use the Down Arrow key y to select an existing Template program from the menu e g 2 Step Template or 3 Step Template New program 2 STEP TEMPLATE 501 I 3 STEP TEMPLATE 502 3 A d to select Press the Enter key lt to accept it The Template is already defined and can be run as it is or easily copied modified and saved under a new name 2 STEP TEMPLATE 501 Run Program View Program AV to select Use the Down Arrow key v to
45. rem Lieferanten in Verbindung Bitte beachten Sie die Entgiftungsbescheinigung die Sie mit dem Ger t erhalten haben 1 Reinigen Bevor Sie Ihr Ger t reinigen sollten Sie zuerst den Netzstecker ziehen das Ger t unter 50 C abk hlen lassen Ein feuchtes Tuch mit Seifenl sung reinigt Ihr Ger t am besten Achten Sie darauf da kein Wasser in das Ger t gelangt Verwenden Sie keine Scheuermittel 2 Sicherungen Die Stromzuleitung ist durch ein oder zwei Sicherungen gesch tzt Diese sollten nur durch qualifiziertes Fachpersonal ausgetauscht werden Wenn die Sicherung wiederholt durchbrennt liegt ein gr erer Defekt vor Das Ger t mu zur Reparatur an Ihren Lieferanten eingesandt werden Espanol Le rogamos lea cuidadosamente la informaci n contenida en este folleto antes de manipular el aparato Stepuridad del usario Todos los usuarios de equipos Techne deben disponer de la informaci n necesaria para astepurar su stepuridad De acuerdo con las instrucciones contenidas en este manual y con las normas y procedimientos generales de stepuridad es muy importante que s lo personal debidamente capacitado opere estos aparatos De no ser as la protecci n que el equipo le proporciona al usuario puede verse reducida Todos los equipos Techne han sido dise ados para cumplir con los requisitos internacionales de stepuridad y traen incorporados un sistema de desconexi n en caso de sobretemperatura En algunos modelos el sistema de desco
46. rement the temperature of a specified step of a programme Temperature Cycle 1 Cycle 2 Cycle 3 Cycle 4 Cycle 5 Temperature profile of incremented Hold Temperature at step 3 of each cycle The Hold Temperature of the incremented decremented step is the defined Hold Temperature plus the summation of the increments decrements The first cycle is never incremented decremented 29 30 only subsequent cycles If you select a decrement the Hold Temperature is prevented from falling below 4 C If you select an increment the Hold Temperature is prevented from rising above 99 C Similarly under normal circumstances the Hold Time of all steps is constant However it is possible to automatically increment or decrement the duration of a specified step of a program Temperature Cycle 1 Cycle 2 t 20t Cycle 3 t 40t Cycle 4 Cycle 5 Temperature profile of incremented Hold Time at step 3 of each cycle The Hold Time of the incremented decremented step is the defined Hold Time plus the summation of the increments decrements The first cycle is never incremented decremented only subsequent cycles If you select a decrement the Hold Time is prevented from falling below one second Number cycles 30 Step 94 0 C 00m30 max Step 58 02C 00m30 max AV d to edit lt to delete step to insert step Number cycles 30 Step 94 0 C 00m30 max Step 58 02C 00m30 max CHANGE STEP
47. s first switched on Fuses If neither the power light nor display on the front panel is lit one of the two fuses may have blown Check that there is no external cause such as a faulty plug or lead Check both fuses and replace the faulty fuse with a new one of the correct value fuse values are given on the label next to the power inlet Note that fuses should only be replaced by a qualified electrician The holder for the two fuses is built into the mains input socket First remove the power cable and then gently prise the fuse drawer open with a flat bladed screwdriver or similar tool Each fuse can be removed by using the screwdriver as a lever Exchange the faulty fuse in the fuse holder for a working fuse of the correct value Finally replace the fuse drawer in the fuse compartment and push the drawer shut Fuses which blow repeatedly indicate a serious fault and you should return the unit to your supplier for repair Interchangeable blocks The block can be removed and replaced by another of the same sort or of a different sort The software will register the change and set up the unit for the new block Remove the four screws on the underside of the unit and drop the block fan assembly down Unplug the assembly from the wiring and remove the assembly from the unit Refitting the block fan assembly is the reverse procedure be careful of the wires as you refit the block MS Fo E AM00000000000 Underside of th
48. t of misuse accident or negligence Servicing under this guarantee should be obtained from the supplier Notwithstanding the description and specification s of the units contained in the User s Manual Barloworld Scientific hereby reserves the right to make such changes as it sees fit to the units or to any component of the units This Manual has been prepared solely for the convenience of Techne customers and nothing in this Manual shall be taken as a warranty condition or representation concerning the description merchantability fitness for purpose or otherwise of the units or components User maintenance NOTE THAT THIS EQUIPMENT SHOULD ONLY BE DISMANTLED BY PROPERLY TRAINED PERSONNEL REMOVING THE SIDE FRONT OR REAR PANELS EXPOSES POTENTIALLY LETHAL MAINS VOLTAGES THERE ARE NO USER MAINTAINABLE PARTS WITHIN THE EQUIPMENT In the unlikely event that you experience any problems with your unit which cannot easily be remedied you should contact your supplier and return the unit if necessary Please include any details of the fault observed and remember to return the unit in its original packing Barloworld Scientific accepts no responsibility for damage to units which are not properly packed for shipping if in doubt contact your supplier See the Decontamination Certificate supplied with your unit 1 Cleaning Before cleaning your unit ALWAYS disconnect from the power supply and allow to cool below 50 C Your unit can be cleaned by wiping w
49. the Admin password which allows access to Configuration and also to delete any passwords associated with programs Highlight Passwords then press Enter lt The screen will appear as opposite We suggest that you change the Admin password at this stage in order to protect the configuration settings Use the Up Arrow A key to highlight Admin passwd Press Enter lt and type in the new password Repeat to confirm and accept The screen will go back to the Configuration menu To delete a password associated with a program use the Up Arrow and Down Arrow y keys to highlight the program Press Enter lt Press Enter d again to delete the password 37 Controller reset The Controller reset will delete all saved Program defaults programs from the unit Settings Passwords Highlight Controller reset then press Enter Controller reset CONTROLLER RESET To delete all the programs press Enter lt This will delete alll programs SURET d YES V NO 9 Running the TC 3000 from a PC The TC 3000 can be run from a PC using Techne s Gensoft software This is available to download from the Techne web site at www techne com You will need Winzip which is available free of charge on www winzip com to unzip the files Instructions for using Gensoft are included in a separate Manual Connecting one unit to the
50. to the computer using the daisy chain cables 38 Upgrading the software in your TC 3000 oooccccccnccccccncnnnnnnnnnnnnnnnnnnnnnnnnnnnnoninininons 39 10 Notes on transferring protocols from other thermal cyclers 40 11 User Maintenance Ae 41 Cleaning your TC 3000 2 41 Heater BIOCK removal rota 41 12 Some frequently asked questions 42 13 Additional information 43 Fault FINdiNO ratio diodo plo ne plo pio pudo plot pudo loca nh 43 POSSE 43 Interchangeable blocks 5 3 2 Heart 43 AECESSOMO Sor AS DA A RA AAA AAA AAA A 44 Replacement paris ni ts be 44 1 Introduction The TC 3000 provides the researcher with the means of accurately controlling the temperature profile of samples lt has many scientific applications including DNA amplification and sequencing The TC 3000 can cycle samples between 4 C and 99 C The TC 3000 is programmed by means of an integral keypad and LCD display A program which can be recalled from memory consists of e a series of specified temperatures in C e the times for which each specified temperature will be held Hold Times e the desired heating or cooling rates in C sec between each specified temperature e optional instructions as to whether the times and or the temperatures are to increase or
51. u have made will be lost The program is now ready to run and will be allocated with a unique Speed dial number which can be used to rapidly locate the program from the main start up screen Up Arrow or Down Arrow keys to scroll through the remaining list of options To edit a field or step in an existing program Programs Tnformat ion Conrieurarfion IV d to select DAVID DONNA d to select DONNA Run program View program Edit program Copy program Delete program Set password tv d to select Edit a selected field or step DONNA Heated Lid TOSSE Preheat Lid on INV to edit V to save When the unit is switched on the display will show this screen If known type in the Speed dial number for the required program and press Enter This will take you directly to the required program If you do not know the Speed dial number press Enter to access the list of stored programs Either 1 Scroll to the required program using the Up Arrow or Down Arrow keys v or 2 Type the first letter of the program name in the New program field this will then list all the program names beginning with that letter Use the Up Arrow or Down Arrow keys N to select the required program Press Enter to accept lt The full set of options for a program is shown here these are shown on the LCD screen thr
52. una garant a condici n o aseveraci n de la descripci n comerciabilidad o adecuaci n para cualquier fin espec fico de los aparatos o sus componentes Mantenimiento ESTE APARATO DEBE SER DESMONTADO SOLO Y EXCLUSIVAMENTE POR PERSONAL DEBIDAMENTE CAPACITADO EL RETIRAR LOS PANELES LATERALES FRONTALES O TRASEROS SUPONE DEJAR AL DESCUBIERTO TENSION DE LA RED PELIGROSA EL EQUIPO NO CONSTA DE NINGUNA PIEZA DE CUYO MANTENIMIENTO SE PUEDA ENCARGAR EL USUARIO En el caso improbable de que experimentara alg n problema con su aparato que no pudiera resolver con facilidad deber a ponerse en contacto con su proveedor y devolverlo si fuera necesario Indique de forma detallada todos los defectos que haya notado y devuelva el equipo en su embalaje original Techne no aceptar responsabilidad alguna por da os causados en equipos que no estuvieran debidamente embalados para su env o si tuviera alguna duda p ngase en contacto con su proveedor S rvase consultar el Certificado de Descontaminaci n suministrado con su aparato Limpieza Antes de limpiar su aparato descon ctelo SIEMPRE de la fuente de alimentaci n y permita que se enfr e por debajo de los 50 C Este aparato se puede limpiar pas ndole un pa o h medo enjabonado H galo con cuidado parae evitar que caiga agua dentro del mismo No utilice limpiadores abrasivos 2 Fusibles 11 12 Su aparato est protegido por uno o dos fusibles S lo deben cambiarlos personal debida
53. ure has been reduced to as little as 1s This ensures that the sample is only subjected to the exact temperature required Taking the above into consideration it must therefore be noted that care must be taken when re optimizing protocols from a slower machine Trouble Shooting 1 The optimum annealing temperature may in fact be higher than the set temperature used in the slower machine Try increasing it by 1 or 2 C 2 The hold time can be reduced once the optimum temperature has been found Try reducing it by 5 to 10s 40 11 User maintenance Cleaning your TC 3000 The heating cooling block including wells and flat surfaces should be cleaned regularly to ensure optimum heat transfer to the samples Always clean the block if there has been a spillage Use a cloth or cotton buds dipped in a fresh 50 50 water isopropanol solution and make sure that no deposits are left in the wells In the case of radioactive spillages the heater block can be removed from the unit for more detailed cleaning Barloworld Scientific recommends that you use a proprietary cleaning agent and follow the manufacturer s instructions The heating cooling block is made of aluminium therefore an agent such as Neutracon from Decon Labs Ltd suitable for nonferrous metals should be used However remember that other parts of the unit are made of ferrous materials and may be damaged by spillage onto them Heater Block removal Before cleaning your unit disconne
54. x dont la plaque indique 120 V peuvent fonctionner sur 110 V Dans les deux cas cependant le capacit de chauffage diminuera d environ 8 La plaque d identification se trouve l arri re de l appareil 3 Raccordez le c ble d alimentation la prise situ e l arri re de l appareil 4 Placez l appareil sur un plan de travail ou surface plane ou le cas ch ant dans une hotte d aspiration en s assurant que les trous d a ration situ s sous l appareil ne sont pas obstru s 5 Les symboles ci dessous situ s c t des t moins lumineux sur la face avant de l appareil ont la signification suivante t moin d alimentation 6 Les symboles situ s sur ou c t de l interrupteur de l appareil ont la signification suivante O arr t marche Le couvercle de chauffage au dessus du coupe circuit de la temp rature Le couvercle de chauffage est quip d un circuit ind pendant pour le prot ger contre la surchauffe Dans l v nement peu probable d un probl me de surchauffe avec le couvercle l unit est quip e d un fusible thermique qui coupera la puissance sur le plat de r chauffeur si la temp rature maximale est exc d e Essai d isolation Cet quipement est quip des circuits de suppression d IFR N importe quel contr le de l isolation lectrique l aide du di lectrique haute tension examinant par exemple comme en BSEN 61010 1 doit tre effectu en utilisant seulement une tension DC Cette u
55. ype in the new time The default is minutes 1 For whole minutes type the number required then Enter 2 Totoggle between minutes and hours use the Decimal Point key 3 For values less than 1 minute first press the Decimal Point key followed by the number of seconds required 4 For minutes and seconds type the number of minutes followed by the Decimal Point key then the number of seconds Once the values have been added press Enter d to accept Once you have finished editing and you are happy with the changes press the End key V followed by the Enter key lt to save If you press the End key V a second time for No then all the changes you have made will be lost To copy a selected program UNNAMED Heated lid ISE Preheat lid on AV d to edit V to save To delete a selected program DONNA delete program SURE dd Yes or V No To set a password for a selected program DONNA New passwd g EO wae Ce pic V to exit Option 2 Creating a new program If you select Copy program a screen similar to this will appear and the software will automatically give the program the next number until it is named Press Enter lt and type in a new name You will then be able to edit the program as previously described If you select Delete program a screen similar to this will appear Press Enter lt to delete t
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