DNA Concentrator Kit
Contents
1. Capture and cleaning I of DNA Y Elution of DNA Schematic Procedure for Using the DNA Concentrator Kit ASSAY PROTOCOL Note Always cap spin columns before placing them in the microcentrifuge Before starting prepare the final wash buffer by adding 20 ml of 100 ethanol to CA2 1 Add 2 volumes of CA1 to each volume of the DNA sample and mix well For example add 200 ul of CA1 to 100 ul of DNA sample If required volume is less than 100 ul add 100 ul of CA1 2 Transfer the mixture to the column Centrifuge at 12 000 rpm for 15 seconds Discard the flowthrough Replace the column to the collection tube Note maximum volume of the column is 600 ul 3 Add 200 ul of CA2 final wash buffer to the column and centrifuge at 12 000 rpm for 15 seconds Discard the flowthrough and replace the column to the collection tube Add 200 ul of CA2 to the column and centrifuge at 12 000 rom for 30 seconds 4 Place the column in a new 1 5 ml vial Add 6 8 ul of CA3 directly to the column filter centrifuge at 12 000 rpm for 20 seconds to elute DNA DNA is now ready for use or storage at 20 C RELATED PRODUCTS P 1005 TuMinute PCR Clean Up Kit 110 Bi County Blvd Ste 122 Farmingdale NY 11735 Page 3 Tel 1 877 374 4368 m Fax 1 718 484 3956 m E mail infto epigentek com m Web www epigentek com Printed 2014 05 23 Epigentek Group Inc All rights reserved Products are for research use only P 10062. DNA Concentrator Kit Base Catalog P 1006 PLEASE READ THIS ENTIRE USER GUIDE BEFORE USE The DNA Concentrator Kit is very suitable for concentrating DNA from various DNA samples especially from those with low concentration of DNA such as microdissection samples paraffin embedded tissues restriction enzyme digestions PCR reactions and home made minipreps DNA size is trom 70 bp to 20 kb DNA quantity trom 1 ng to 2 Ug optimal between 10 ng and 1 Ug Concentrated DNA is highly pure and ready for direct use in PCR or other down stream DNA manipulations such as ligation transtormation sequencing restriction digestion microarray analysis labeling microinjection and in vitro transcription 110 Bi County Blvd Ste 122 Farmingdale NY 11735 Tel 1 877 374 4368 m Fax 1 718 484 3956 m E mail infto epigentek com m Web www epigentek com Epigentek Group Inc All rights reserved Products are for research use only Page 1 Printed 2014 05 23 P 1006 KIT CONTENTS Components 50 samples 100 samples P 1006 1 P 1006 2 CA1 Binding Buffer 30 ml 2x 30 ml CA2 Wash Butter 2 5 ml 2x 2 5 ml CA3 DNA Elution Buffer 1 ml 2 mil F Spin Columns 50 100 F Collection Tubes 50 100 User Guide I SHIPPING amp STORAGE The DNA Concentrator Kit can be stored at room temperature 15 22 C for up to 1 year GENERAL PRODUCT INFORMATION Usage Limitation The DNA Concentrator Kit is for research use only and is not intended f
3. or diagnostic or therapeutic application Quality Control Epigentek guarantees the performance of all products in the manner described in our product instructions Product Updates Epigentek reserves the right to change or modify any product to enhance its performance and design A BRIEF OVERVIEW The DNA Concentrator Kit uses a unique procedure and composition to efficiently concentrate DNA from various DNA samples with low concentration of DNA including those from microdissection samples and paraffin embedded tissues The kit has the following features e The fastest procedure available lt 2 minutes e Concentrated DNA is ultra pure with efficient removal of contaminants salts proteins enzymes nucleotides and primers e User friendly binding solution and without chaotropic or other irritant reagents PRINCIPLE amp PROCEDURE The DNA Concentrator Kit simply applies our proprietary DNA binding buffer to DNA sample Atter transterring the sample to the specially designed Fast Spin Column and washing it with the washing buffer the DNA is easily recovered in 8 10 ul with elution solution Concentrated DNA is ready tor down stream application 110 Bi County Blvd Ste 122 Farmingdale NY 11735 Page i Tel 1 877 374 4368 m Fax 1 718 484 3956 m E mail info epigentek com m Web www epigentek com Epigentek Group Inc All rights reserved Products are for research use only Printed 2014 05 23 P 1006 Sample
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