マニュアル Motor Valve MV-8/IMV-8
Contents
1. Angle of rotation 360 360 Dead volume Tul 10 ul Internal volume of flow channel 7 4 ul 30 ul between inlet and outlet Diameter of flow channel 0 6 mm 1 2mm Motor 24V DC 24V DC Connector D type 9 pin on cable D type 9 pin on cable 3 3 Chemical resistance The valve body in contact with the solvent is made of PEEK and PEEK 20 PTFE This is resistant to organic solvents and salt buffers commonly used in a biochemical laboratory The valve can be used in a pH range of 2 13 If very strong acids pH lt 2 or bases pH gt 13 are used the valve should be rinsed immediately afterwards Instructions 56 1153 37 Edition AD Fig 2 Motor Valve MV 8 mounted onto a chromatography rack 4 Installation 4 1 Mounting the MV 8 IMV 8 The valves are fixed in place by screwing the support rod into the valve body and attaching the road to a laboratory rack Mount the valve so the valve body is facing down Fig 2 When mounting valves into an automated FPLC System it is recommended to use the Chromatography Rack Code No 18 1031 75 The chromatography rack is supplied with a number of laboratory rods and clamps which makes the mounting of valves and other accessories simple and convenient To mount the valve in the BioPilot System please refer to the BioPilot System User Manual 4 2 Tubing connection A tubing connection consists of three parts flanged tubing nipple and nipple screw Fig 3 All parts necessary for the t2. Valve MV 8 IMV 8 In this manual the LCC 500 LCC 500 Plus LCC 501 Cl and LCC 501 Plus will be referred to as the controller Only when differences appear will specific names be used 2 Unpacking Please check delivery against the packing list Instructions 56 1153 37 Edition AD 3 3 General Description 3 1 Functional design The Motor Valve MV 8 IMV 8 consists of two main parts the housing which encloses the motor and the electronics and the valve body with a central core The central core rotates 360 inside the valve body As the core in turned by the motor the central port on the bottom of the valve is connected to one of the peripheral ports 1 8 allowing a clear liquid path The peripheral port locations are marked on the valve body directly above each port Valve switching is controlled from the controller by reading the actual position of the valve core by means of signals from a code plate within the valve housing The programmed commands from the controller cause the motor to run until the valve has reached the desired position 3 2 Specifications Motorized 8 position rotary valve used as column solvent and sample selection valve or for fraction collection MV 8 IMV 8 Maximum operating pressure 5 MPa 50 bar 710 psi 2 MPa 20 bar 285 psi Back pressure at 1000 ml hr 0 02 MPa 0 2 bar 2 8 psi Back pressure at 6000 ml h 0 02 MPa 0 2 bar 2 8 psi Wetted material PEEK 20 PTFE PEEK 20 PTFE
3. GE Healthcare Motor Valve MV 8 IMV 8 Instructions Contents 1 Introduction 2 Unpacking 3 General Description 4 Installation 5 Operation 6 Applications 7 Valve maintenance and repair 8 Remote connection 9 Spare parts and accessories ann FW WN 11 12 13 Instructions 56 1153 37 Edition AD 1 Introduction Motor Valves MV 8 Code No 19 7520 01 and IMV 8 Code No 18 4586 01 are motorized valves for automatic selection of columns solvents and samples and for fraction collection The valves have eight different positions Each position connects a central port with one of the eight peripheral ports Fig 1 The Motor Valve MV 8 IMV 8 is controlled from the Liquid Chromatography Controller LCC 500 LCC 500 Plus LCC 500 CI Computer Interface or LCC 501 Plus all of which can control up to six motorized valves MV 8 IMV 8 or MV 7 IMV 7 Motor Valves MV 7 IMV 7 are automatic injection valves The valves can also be controlled from controlling software such as FPLCdirector or UNICORN The main different between MV 8 and IMV 8 is the diameter of the flow channels The diameter of the flow channels for MV 8 is 0 6mm and for IMV 8 is 1 2 mm which allows higher flow rates at lower back pressure MV 8 is normally used in FPLC System IMV 8 is normally recommended for BioPilot System but can also be used in FPLC System 1 Peripheral ports Central port Fig 1 Valve position 1 in a Motor
4. ds During a running method a valve is activated at a certain programmed time or volume or by the monitor signal i e when the monitor signal passes a programmed threshold Allowed positions for the Motor Valve MV 8 IMV 8 are 1 to 8 These numbers correspond to the eight numbered positions on the valve body The home position for the valve is always port 1 If the valve has not been activated manually or within a programmed method the valve is in its home position Instructions 56 1153 37 Edition AD Connect to Columns Connect to Top Bottom Ports al D Port 8 Port 7 Port 7 Port 6 Port 6 Port 5 Ports Port 4 Phenyl Superose 2 Port 4 Port 3 i Sephadex G 25 i Port 3 Port 2 i Port 2 Port 1 o Sephacrul S 300 HR a Port 1 8 1 8 1 7 2 7 2 6 3 6 3 5 4 5 solvent from pump to detector MV 8 IMV 8 MV 8 IMV 8 Fig 4 Two MV 8 IMV 8 valves used for column selection There are two functions which are used when controlling the valves from the controller VALVE POS and STP VALVE 5 2 Function VALVE POS To activate a valve MV 8 IMV 8 enter the valve number corresponding to the socket number on the rear panel of the controller and the position number For example when programming a valve connected to the socket VALVE 2 to switch to position 5 enter VALVE POS 2 5 When selecting a new position the valve core travels the shortest distance to the next position thereby minimizing flow restriction For example if a valve i
5. e to position 2 For further information see the User Manual for respective controller 6 3 Solvent selection The Motor Valve MV 8 IMV 8 can be used for method development in liquid chromatography If the valve is used as shown in Figure 7 up to eight runs with different solvents can be performed automatically since the controller allow automatic solvent changeover Instructions 56 1153 37 Edition AD 9 10 Note With the MV 8 IMV 8 connected before the pump P 500 you should use tubing with large inner diameter to avoid cavitation e g tubing with 1 1 mm i d When connected before Pump P 6000 1 9 mm i d tubing should be used Buffer E Buffer A Buffer F Buffer B Buffer G Buffer C Buffer H Buffer D to pump Fig 7 The MV 8 IMV 8 used for solvent selection 6 4 Fraction collection The MV 8 IMV 8 can also be used to collect fractions from an effluent stream This application is illustrated in Figure 8 When collection fractions it is possible to program a delay time or volume in he controller corresponding to the time or volume necessary for the liquid in the cell of the monitor to reach the tubing outlet By using the commands PS CALL and PE CALL you can collect only the peaks The corresponding commands to PS CALL and PE CALL in UNICORN is found under the Watch instruction Then position 1 can function as a drain or waste port in the same way as rinsing is done in sample injection Section 6 2 allowing effl
6. ia Tel 61 2 8820 8299 Fax 61 2 8820 8200 Austria Tel 01 57606 1613 Fax 01 57606 1614 Belgium Tel 0800 73 890 Fax 02 416 8206 Canada Tel 1 800 463 5800 Fax 1 800 567 1008 Central East amp South East Europe Tel 43 1 972 720 Fax 43 1 972 722 750 e Denmark Tel 45 70 25 24 50 Fax 45 45 16 2424 e Eire Tel 1 800 709992 Fax 44 1494 542010 e Finland amp Baltics Tel 358 9 512 3940 Fax 358 9 512 39439 France Tel 01 69 35 67 00 Fax 01 69 41 98 77 Germany Tel 0800 9080 711 Fax 0800 9080 712 e Greater China Tel 852 2100 6300 Fax 852 2100 6338 Italy Tel 02 26001 320 Fax 02 26001 399 e Japan Tel 81 3 5331 9336 Fax 81 3 5331 9370 e Korea Tel 82 2 6201 3700 Fax 82 2 6201 3803 Latin America Tel 55 11 3933 7300 Fax 55 11 3933 7304 e Middle East amp Africa Tel 30 210 96 00 687 Fax 30 210 96 00 693 Netherlands Tel 0800 82 82 82 1 Fax 0800 82 82 82 4 e Norway Tel 47 815 65 777 Fax 47 815 65 666 e Portugal Tel 21 417 7035 Fax 21 417 3184 Russia amp other C I S amp N I S Tel 7 495 956 5177 Fax 7 495 956 5176 e Spain Tel 902 11 72 65 Fax 935 94 49 65 Sweden Tel 018 612 1900 Fax 018 612 1910 Switzerland Tel 0848 8028 10 Fax 0848 8028 11 UK Tel 0800 515 313 Fax 0800 616 927 e USA Tel 1 800 526 3593 Fax 1 877 295 8102 imagination at work 56 1153 37 AD 12 2006 E Elanders sterv la 2006
7. id path from the pump to the detector Note Do not mount the valve with the valve body facing up Instructions 56 1153 37 Edition AD 6 2 Multiple sample injection Together with the motorized injection valve MV 7 IMV 7 one MV 8 IMV 8 can be used for automatic sample injection of up to 8 samples With two MV 8 IMV 8 valves the number of samples can be increased to 15 fig 5 If rinsing is required between sample injections use the tube in position 1 for the rinse eluent as described below 1 Valve 3 MV 8 IMV 8 in position 1 rinse Fig 6a 2 By executing the function STP VALVE 3 the valve moves to port 2 and injects the sample located there Fig 6b 3 By executing the function VALVE POS 3 1 the valve returns to position 1 rinse and performs a rinse cycle Fig 6c 8 8 1 Rinse 7 2 7 a 2 6 3 6 3 5 4 to MV 7 IMV 7 to MV 7 IMV 7 Fig 6a Valve position 1 Fig 6b STP VALVE 3 8 1 Rinse 8 1 Rinse 7 YA 2 7 2 6 3 6 3 5 4 5 4 to MV 7 IMV 7 to MV 7 IMV 7 Fig 6 Two MV 8 IMV 8 valves used in combination with an MV 7 IMV 7 valve for automatic sample injection of up to 8 or 15 samples 4 At the next STP VALVE 3 command the control unit remembers that the previously injected sample was located at port 2 and advances directly to position 3 Fig 6d 5 The STP VALVE function is reset by the instruction VALVE POS 3 8 or at the end of the method The next time STP VALVE is executed after a reset the valve will mov
8. low min 3 V Indicates the valve address BIT 0 See also table below Instructions 56 1153 37 Edition AD Codes for the 8 valve positions are shown in the table below Note The motor Valve MV 8 IMV 8 is designed to be controlled by the controller If the MV 8 IMV 8 is controlled from other equipment e g a microprocessor a 10kQ pull up resistor to 5 V must be connected to pins 3 7 8 and 9 of the 9 pin D SUB connector of the MV 8 IMV 8 Position Address Position BIT 2 BIT 1 BITO match 1 0 0 1 0 2 0 1 0 0 3 0 il 1 0 4 1 0 0 0 5 1 0 1 0 6 1 1 0 0 7 1 1 1 0 8 0 0 0 0 1 3 5 0 0 1 5V 9 Spare parts and accessories Accessories MV 8 Code No Designation No per pack Capillary tubing o d 1 8 mm i d 0 5 mm 2m 19 7477 01 Tubing connectors 5 19 7476 01 Flanging Start Up kit 120V 1 19 5079 01 220 V 1 19 5090 01 Motor Valve MV 7 1 19 7510 01 Spare Parts MV 8 Code No Designation No per pack Valve V 8 kit 1 19 7806 01 Instructions 56 1153 37 Edition AD 13 14 Accessories IMV 8 Code No Designation No per pack Tubing i d 1 2 mm o d 1 8 mm 2m 19 4370 01 Tubing connectors 1 8 5 19 7476 01 Tubing i d 1 9 mm o d 2 7 mm 2m 18 8207 01 Tubing connectors 2 7 5 18 4652 01 Flanging Tip Kit i d 1 2 18 4597 01 Flanging Tip Kit i d 1 9 1 18 4596 01 Flanging start up Kit for BioPilot 120 V 18 4603 70 Flanging start up Kit for BioPilot 220 V 18 4603 71 ot
9. or Valve IMV 7 18 4585 01 Superloop 150 ml 19 7850 01 Spare Parts MV 8 Code No Designation No per pack Valve Kit IMV 8 1 18 4595 01 Instructions 56 1153 37 Edition AD Instructions 56 1153 37 Edition AD 15 WWW gehealthcare com Sepharose is a trademark of GE Healthcare companies GE imagination at work and GE monogram are trademarks of General Electric Company GE Healthcare Bio Sciences AB All goods and services are sold subject to the terms and conditions of Bj rkgatan 30 sale of the company within GE Healthcare which supplies them GE Healthcare reserves the right subject to any regulatory and 75184 U ppsala contractual approval if required to make changes in specifications Sweden and features shown herein or discontinue the product described at any time without notice or obligation Contact your local GE Healthcare representative for the most current information 2006 General Electric Company All rights reserved GE Healthcare Bio Sciences AB a General Electric Company GE Healthcare Europe GmbH Munzinger Strasse 5 D 79111 Freiburg Germany GE Healthcare UK Ltd Amersham Place Little Chalfont Buckinghamshire HP7 9NA UK GE Healthcare Bio Sciences Corp 800 Centennial Avenue P O Box 1327 Piscataway NJ 08855 1327 USA GE Healthcare Bio Sciences KK Sanken Bldg 3 25 1 Hyakunincho Shinjuku ku Tokyo 169 0073 Japan Asia Pacific Tel 85 65 62751830 Fax 85 65 62751829 e Australas
10. re parts in the Valve V 8 kit Code No 19 7806 01 Valve Kit IMV 8 Code No 18 4595 01 Replace these plates when worn Valve kit V 8 Valve kit IMV 8 E Valve axel i Distruting plate Allen screw 1 Central port a 1 l i 3 Channel plate Stainless steel plate Fig 9 Disassembled Motor Valve MV 8 IMV 8 Note When reassembling the valve be sure that the channel plate connects the central port with the peripheral port number 1 The four Allen screws on the bottom of the valve should be tightened equally in turn until the bottom plate is fixed to the valve body 8 Remote connection The following section describes the function and activation state of the pins in the remote connections Active Pin voltage Function 1 24 V Supplies power to the motor and relay For power consumption see pins 2 and 6 2 0 Controls the valve rotation Sink min 30 mA for clockwise rotation 3 0 1 5 V Indicates the valve position match When active in position the valve position can be read on pins 7 8 and 9 address pins 4 Signal ground voltage reference for the signals listed in this table 5 V Supplies power to the LEDs in the valve Min 80 mA 0 Controls the start stop function of the motor Sink min 1 A to start then approx 0 3 A while running Short circuit to pin 1 to stop 7 min 3 V Indicates the valve address BIT 2 See also table below min 3 V Indicates the valve address BIT 1 See also table be
11. s in position 2 and the next setting is to position 7 the valve core moves counter clockwise 5 3 Function STP VALVE With this function the MV 8 IMV 8 can be made to step forward clockwise to the next valve position If valve 5 is in position 3 and the function STP VALVE 5 is executed the valve steps forward to position 4 The controller remembers the last position reached by the STP VALVE function even if the VALVE POS function is used between Examples of how this feature is used in multiple sample injections and fraction collection are described in Section 6 Applications Instructions 56 1153 37 Edition AD 7 For further information about valve control consult the User Manual for respective controller Peristaltic Pump P 1 Valve 1 Valve 2 Valve 3 Peristaltic Pump P 1 Fig 5 One or two MV 8 IMV 8 valves used with an MV 7 IMV 7 valve for automatic sample injection 6 Applications Several examples of the use of the MV 8 IMV 8 as a selection valve are given below When used in this manner the MV 8 IMV 8 condenses laboratory equipment into less physical space and saves time with faster more efficient change over of columns pumps detectors solvents or samples 6 1 Column selection In Figure 4 two MV 8 IMV 8 valves are used to connect eight different columns to a single pump and detector Every time a position is changed with valve A the corresponding change must be made with valve B to have a clear liqu
12. ubing connection with instructions for use are supplied in the Flanging Start up Kit 120 V Code No 19 5079 01 or 220 V Code No 19 5090 01 and in the Flanging Start up Kit for BioPilot 120 V Code No 18 4603 70 or 220 V Code No 18 4603 71 is necessary If you are using tubing other than that supplied the flange should have a flat smooth surface and a diameter of approx 3 mm When connecting the tubing to the valve ports finger tighten the tubing connectors into the valve body and the turn them a further 45 with the plastic wrench supplied Note Do not use standard wrenches to tighten the tubing connectors Do not overtighten the tubing connectors they are designed to seal up to 100 bars when flanged properly If the connector leaks after it has been tightened with the plastic wrench check the flange and make a new one if needed Instructions 56 1153 37 Edition AD 5 Tubing Fig 3 Tubing connection 4 3 Connection to the controller Connect the valve to the controller by plugging the D SUB connector into one of the sockets on the rear panel if the controller marked VALVE 1 6 4 4 Valve position guide Attach the self adhesive sticker which is supplied with the valve to the valve housing see Fig 2 It provides a handy reference to the connection scheme 5 Operation 5 1 Programming Valve switching is controlled from the controller or from controlling software either by manual settings or via programmed metho
13. uent between peaks to be diverted to a separate container Fig 8 For further information see the Applications section in the User Manual for your controller Instructions 56 1153 37 Edition AD Fraction 8 y Fraction 74 7 ion 2 ion 3 Fraction 6 6 Fraction sy 5 4 i Fraction 4 Fig 8 The MV 8 IMV 8 used for fraction collecting 7 Valve maintenance and repair The valve can be cleaned either by pumping solvents through it or if that is not efficient enough by disassembling the valve and placing the channel p late and the distributing plate in an ultrasonicator Suitable cleaning solvents are water ethanol 75 acetic acid 0 2 M NaOH and detergents When using cleaning agents other than water always rinse the valve with water as the final step Disassembly see Figure 9 1 2 Set the valve to position 1 before disconnection it from the control unit Remove the four Allen screws on the bottom of the valve Loosen each one equally in turn until the bottom plate comes off parallel to the valve body Slide these screws out with the stainless steel plate Remove the distributing plate containing the 8 ports Remove the channel plate Instructions 56 1153 37 Edition AD 11 12 6 Place the channel plate and the distributing plate Fig 9 into an ultra sonicator with an appropriate wash solution These two parts are also the only parts likely to need replacement They are supplied together as spa
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