LumaSpec 800 User Manual
Contents
1. JESRSESRC GR ss SBS als sie 5 I ologo oWo o Wo oWo i ea IAKO CAKO KARG SHT 3 gt 3 3 3 ag 5 ka HH ejo Sola D o EP B et amp E N e ba bd far MEE s Page 19 Overview Continued UPPER DATA BAR The upper data bar has the Tools Save Dark Spectrum Export to Excel Import Spectrum from Excel Data Collection Start Change Integration Time and Help buttons Information on the functionality of these button are detailed separately Upper Data Bar LOWER DATA BAR The lower data bar displays the overall spectral power for the illumination system the spectral power at a single wavelength chosen by clicking on the graph and the maximum raw data value Viewing the maximum raw data value is useful when choosing the integration time for each illumination system 262 210 15 Single Wavelength Selected Uem ZnT 650 0 700 0 750 0 200 0 Overall Spectral Power Wavelength B743 a 102 747 I a ae Maxi Raw Data Val Overall Spectral Power aximum Raw Data Value Spectral Power at Chosen Wavelength Section 9 SETTING RANGES 1 THROUGH 10 Ten user defined wavelength ranges are available Clicking on the check box in each range allows you to view the range on the main graph Two color coded vertical lines show at a glance the defined range The overall spectral power for the range selected is displayed below the range name2. Je 7 2 INS h I E um LI Objective Light is Off Center Light is On Center of Crosshair Use the microscope focus mechanism to focus on the crosshair inscribed on the surface of the Illumination Target It is recommended to start with your lowest magnification objective when trying to find and focus on the crosshair Note that if white light is not available the coating on the Illumination Target will allow you to view and focus on the crosshair with many of the common fluorescent filter sets Carefully remove the Illumination target and turn it over with the aperture side up At this point the Illumination Target is ready to use Note if the manual stage moves while you are turning it from the crosshair side to the aperture side up you will need to redo the crosshair alignment and focus adjustment Upright Microscopes Without Integrated Slide Holder Place the Illumination Target onto the manual stage with the aperture side UP Adjust the position of the XY stage until the light from the objective falls centrally on the aperture of the Illumination target When centering the aperture it may be helpful to minimize the spot of light coming from the objective by adjusting the aperture stop so that the light coming from the objective is similar in size to the crosshair Open the aperture stop completely after alignment is finished Raise the illumination target until the top of the target is at the focus posit
3. Save Dark Import Spectrum Data Collection ort to Excel integration Time i r a Change Integration 262 User Defined Wavelength e Range Spectral Range Power 400 0 450 550 0 600 0 650 0 700 0 750 0 Wavelength nm The range name can be changed by right clicking on the name and renaming The new name can include alphanumeric characters but spaces are not allowed The color of the range can be changed by clicking on the color chip and selecting a basic color or clicking on the color pallet Save Dark Import Spectrum Data Collection ort to Excel S x ti f KRER T bare Change Integration cientific 4 ms Time 5 oi Range 1 2583500 MULU pa o 0 0 Ese 400 0 gt i Range 2 Min 401 0 nm i go TAH era 450 0 Mal pem D ik Right Click to Change Range Name Min 501 0 nm ira 550 0 E nm Click Here to Change Color t M n 651 0 T nm M ax 700 0 n DO 05 i O ed 400 0 450 0 500 0 550 0 600 0 700 0 ee 721 0 MOI go era 750 0 S Eaa Wavelength nm Min aie nm Overall Spectral P Maximum Raw Data 1 eral Spectral Power z aximur aw Vals VERI 800 0 H aa a im 103 256 10937 Spectra Power 99 6 nm M n 703 U nm aut dre 720 0 nm 0 mm Page 21 Setting Ranges 1 through 10 Continued The minimum and maximum ranges can be increased and decreased using the up down arrows or by typing numbers
4. D mE Qi i e Section 3 PRODUCT FEATURES Major features of the LumaSpec LS800 include e llumination target in 3 x 1 slide format measuring spectral power at the sample plane 1 meter fiber optic cable e Small footprint of 13 5 square inches e 350 800 nanometer range e 1 5 nanometer resolution e Software with irradiance and transmission modes Graphical and numerical spectral power data 10 selectable ranges for spectral power at wavelengths of interest Time stamped spectral power data collection function Export and import functions USB powered Section 4 GETTING STARTED Identifying the Components of your System Check to see that all the required components have been included with your LumaSpec LS800S compact illumination spectrometer The standard LumaSpec 800 system consists of 1 LumaSpec 800 Housing LM407 1 One Meter Fiber Optic Cable W4221 1 Illumination Target LM408 1 USB Cable 1 Quick Start Guide 1 Software Installation CD LumaSpec 800 Housing One Meter Fiber Optic Cable LUMASPEC 800 QUICK START INSTRUCTIONS h PLEASE READ THE INSTRUCTIONS BEFORE 4 Illumination Target ATTEMPTING TO USE THE PROSCAN SYSTEM 4 USB Cable Software Installation CD es z 3 Pe TORI Tul Free DORATT 2294 Fas TRA Seb mem tr d m Quick Start Guide Section 5 SOFTVVARE INSTALLATION Note You must complete the following software instal
5. VAT rw 6000 KR 6010 nm tje ne m D e ag sr C 1 c i Sill 5 e gapa 3 I j NI FT m ai S A 1 1 j 550 0 600 0 Wavelength nm o B E vera De 111 481 RH 3 de gt W y a NE I I I I i A ME C I D D oi I I I j i I Soa 3 i I Tami N M gm pim Os os ri it T m i a jow od L c ib o 8 o sja a r B i 3 3 Page 33 Section 12 TROUBLESHOOTING If you experiencing problems while trying to run the LumaSpec software performing a complete uninstall and reinstall of the software may alleviate the problem you are having Please follow the procedure below to ensure all of the program components are uninstalled and then reinstall the software using the installation procedure provided in the manual Open your program list from the control panel For Windows 7 Go to the start menu and click on Control Panel Select Programs and Features in the control panel For Windows XP Go to the start menu and click on Control Panel Select Add or Remove Programs in the control panel Uninstall the following programs from the list of installed programs e LumaSpec Microsoft NET Framework 4 Client Profile Microsoft Visual C 2010 x64 Redistributable This program may be installed as the x86 version depending on the operating
6. Range 7 Range 8 Range 9 Range 10 401 451 501 551 601 651 703 721 751 Min nm Max nm Spectral Power 350 400 2173 65 Page 27 Import Spectrum from Excel Overlays previously saved spectrum See Export to Excel over live graph Up to four spectrums can be overlayed simultaneously Imported Spectrum UOT ANT Live Spectrum 550 0 600 0 Wavelength nm Data Collection Start Saves time stamped spectral power data for spectral ranges 1 10 Clicking Data Collection start requires the user to select a location to save the data then immediately begins collecting data Pressing Data Collection otop will end date collection immediately in progress DC Count A5 DG Time Stamp 1 8 2013 4 07 22 PM In Progress will flash during the data collection process and Data Collection Complete will pop up when the process Is finished Vata Lollectan Lomplete press UR to contine Change Integration Time Allovvs the user to change mote time E r ci E a g zy Overall Spectral Powe 14 947 Units can be seconds milliseconds or microseconds 550 0 600 0 Wavelength nm Max imum Raw Lata 2854 Change Integration Time Continued Higher integration times will increase the signal to noise ratio and provide more stable spectral power information Increasing integration time will increase the maximum raw data value Integration time must be decreased if maximum raw data va
7. before pressing OK il At this point the user needs to block the light going to the spectrometer Blocking the light can be accom plished by closing a shutter turning off the light source or even blocking the input aperture Please note if you move the illumination target while blocking the light it will need to be relocated so that the light from the objective falls centrally on the input aperture Unblock the light source to the LumaSpec and the LumaSpec Illumination Spectrometer and software are ready to use Section 8 LUMASPEC DISPLAY SCREEN Overvievv MAIN SCREEN The LumaSpec software main screen provides detailed information for the illumination systems at a glance A graphical representation of the current illumination spectrum is provided centrally located in the main screen E CER JJ a caia Om nis FES E eizgziz oio olog id d sa mim olo ce i gt uWicm2 nm mata oo ce sp gj e m NE B mu e Lp BU Ki X m T u y 5 JE ZI gang LJ S eco all Spectral Povver 103 248 The three buttons located at the top of the graph are for rescaling the graph forth between transmission and irradiance au modes To the left of the graph are 10 selectable wavelength ERES which will provide spectral power information for user selectable wavelengths ranges of interest r and toggling back and A EB ce E sj eo i v
8. checking filter transmission efficiency and remaining lifetime PRIOR s Time Sitte DETIT a E K B a DEG 1 I n NR i E E KU g P ay S _ olo KARG dema tet NET 3 I b a i er 3 I Click here to switch to transmission mode i gt uW cm2 nm E ES E gapa ZU i M I 550 0 600 0 Wavelength nm E 0E E I ETT d AW LE iO I I Po h A MM J JEFIKE I gi z I I M 1 I NIE EIE GJ b i i l c 1 I il 3 ae e j 1 J 3 3 I 11 J N o oo jU J 3 Li D I e e 3 3 2 j 3 1 gm co a The graph below shows a halogen light source with a filter passing approximately 75 of the available light between 510 555 nanometers Note The large amount of noise in the lower wavelength range is a byproduct of the transmission calculation and indicates very low output in this range before inserting the filter Transmissioni 6 Page 32 Absolute Irradiance Measurement Mode E Default LumaSpec operating mode In this mode the LumaSpec measures Absolute Irradiance in microwatts per square centimeter per nanometer Absolute Irradiance Measurement m EEN u fap m AE 5 Il c 4 BE E om o LI 138313 Irradiance Units A mi Nm NEN E di D Hu 3 e dr Il 3 AAW Min 5510
9. directly into the min max ranges Min 350 005 Use Arrovvs to Increase or Decrease Value M rix imir Min 401 0 TUT Weed 450 0 SN Users Can Also Type Value Directly Into Field Dali LEETE a IM TRE Range 3 Min 151 Mar c Hange 4 B Range 5 71 0 Max 600 zd z JL I c ce ql F Range 6 Min f j E E Bp Is Mrd DE kul If Min Max act E BE B B I woh ca n Min ieee 5D mm mima LENI ca Balog en Range 11 Min m ng Max in N T DD LATA I co ca FLT Section 10 TOOLS Clicking on the tools button allows the user to modify Data Collection Settings increase or decrease Scans to Average increase or decrease the Default Wavelength main display open previous program settings save current program settings Connect to the LumaSpec spectrometer and Use Initial Dark Spectrum Lata Gallectian Settings Scams to Av B Seconds X Open Save Use initial Dark Spectrurr Settings Settings Scans to Average opecifies the number of spectral acquisitions that the device accumulates before the LumaSpec program displays a revised spectrum The higher the value the better the signal to noise ratio but the slower the response Scans to Average Field Data Collection Settings Frequency Use initial Dark Spectrum Default VVavelength Allows the u
10. spectrum graph Page 36 Issue Saving a new Dark Spectrum zero calibration is inconvenient each time Integration Time is adjusted Data Collection Settings Frequency Duration 1 FE asl Duration a SECON S n E nm Ini Suggestion In the Tools menu check Use Initial Dark Spectrum and the software will not require a new Dark Spectrum every time Integration Time is changed Page 37 Section 13 RETURNS AND REPAIRS Should you experience problems with your LumaSpec 800 and want to send it back for service warranty or otherwise a Return Material Authorization RMA number must be obtained from the appropriate Prior Scientific office before returning any equipment For North and South America contact Prior Scientific Inc and for the rest of the world call Prior Scientific Instruments Limited on the telephone numbers shown below Worldwide Locations Prior Scientific Ltd Prior Scientific Inc Prior Scientific GmbH Prior Scientific KK Cambridge UK Rockland MA USA Jena Germany Tokyo Japan T 44 0 1223 881711 T 1 781 878 8442 T 49 0 3641 675 650 T t81 3 5652 8831 E uksales Oprior com E info prior com E jena prior com E info japan prior com Prior Scientific recommends having the LumaSpec 800 calibrated annually to ensure reliable and accurate performance Contact your local Prior Scientific office for more information
11. 3 l So Select Installation Folder The installer will install LumaS pec to the following folder To install in this Folder click Next To install to a different folder enter it below or click Browse Folder C Program FilessPrior ScientifickLumaSpec Browse Disk Cost Install LumaSpec for yourself or for anyone who uses this computer um Click Next in the confirmation window in order to begin the installation EU Mt gt Confirm Installation The installer is ready to install LumaSpec on your computer Click Nest to start the installation Installation Complete Lumes pec has been successfully mstalled Click Close to exit Please use Windows Update to check for any critical updates to the NET Framework Step 4 Install the drivers The driver installation vvill begin automatically and install vvithout any required action from the user After the driver installation has completed the computer will need to install the C 2010 x64 Redistributable as part of the driver installation Note If you are running a 32bit computer the C 2010 x86 Redistributable will be installed in place of the x64 version If this program is already installed on your computer you will get the window below In this situation simply click Cancel and then click Yes to confirm and the installation process will complete You can repair your installation or remove it from
12. PRIOR Cc ie n t Lumaspec 800 U ser Manual PRIOR Scientific Prior Scientific Ltd Prior Scientific Inc Cambridge UK Rockland MA USA T 44 0 1223 881711 T 1 781 878 8442 E uksales prior com E info prior com Prior Scientific GmbH Jena Germany T 49 0 3641 675 650 E jena prior com Prior Scientific KK Tokyo Japan T 81 3 5652 883 1 E info japan prior com TABLE OF CONTENTS PAGE Section 1 Safety Information 1 Section 2 Product Overvievv 2 Section 3 Product Features i ZS Section 4 Getting Started 4 Section 5 Software Installation 5 Section 6 Hardware Installation 11 6 1 Connecting Fiber Optic Cable 6 2 Connecting USB Cable 6 3 Setting up the Illumination Target Section 7 Initial Use of LumaSpec Software J 16 Section 8 LumaSpec Display Screen 48 Section 9 Setting Ranges 1 through 10 2 Section 10 Toos gt 1 2 2 2 D 22 10 1 Scans to Average 10 2 Default Wavelength 10 3 Data Collection Settings 10 4 Use Initial Dark Spectrum 10 5 Open Settings 10 6 Save Settings 10 7 Connect 10 8 Save Dark Spectrum 10 9 Export to Excel 10 10 Import Spectrum from Excel 10 11 Data Collection Start 10 12 Change Integration Time Section 11 GraphFunctions VV 30 11 1 Scale Graph to Fill Window 11 2 Transmission Mode 11 3 Absolute Irradiance Measurement Mode Section 12 Troubleshoot
13. een 14 000 and 16 500 decrease the integration time until the value falls between 13 000 and 14 000 Please note that the minimum value that can be input is 1 Decimals are not allowed The units of integration time can be changed from microseconds us to milliseconds ms to seconds Decreasing the integration time will lower the maximum raw data value increasing the integration time will raise the maximum raw data value If the LumaSpec sensor is saturated with too much light the maximum raw data value will sit at approximately 16383 The maximum raw data value is continuously displayed in the lower data bar see below A red warning icon will flash in the right hand side of the data bar if the maximum raw data value exceeds 15 000 Integration Time Integration Time E C E S 3 Integration Time Units Warning that maximum raw data is too high 400 0 450 0 500 0 550 0 600 0 650 0 1 750 0 800 0 Maximum Ravv Data Wavelength nm Lower Data Bar zi EU Maximum Raw Data inm j he sensor is saturated and a lower integration 16383 dh ime needs to be chosen Automatically Setting Integration Time To set integration time with the automatic feature click Yes A window will appear Automat IPRC ration rnc Adjustment in proqrese picase vall E The LumaSpec software will automatically adjust the integration time until the value falls between 13 000 and 14 000 A new window will appear Spectrometer
14. ged on the main display which allows the LumaSpec to quantify light sources from the dimmest LED to the most intense mercury source The user can overlay multiple saved spectrums up to four over the live graph to quickly compare illumination performance over time The LumaSpec software will save and import settings for different illumination sources making calibrating multiple optical systems quick and easy Collecting and saving time stamped spectral power data is accomplished with a click of the mouse In Transmission Mode rJ the LumaSpec software monitors and graphs 100 light output for the illumination system and then determines the wavelength and the percent transmission for each filter placed into the light path This feature is extremely useful in determining the overall efficiency and remaining lifetime of optical filter sets ntegration Time 4 ms p Cil dn auilion tific Pine BL x 7 oOo fF eS a5 es 5 B ES ES aa HE EIE BololNoollNo o tako koka caka ED E MEN INN p a p at a EU LJ Qn si oo Is E E C E 2 a SE p a f 3E HI p o sr SC JE E LJ sales oloo ol aka caka TEM n m D EN IL TI ai amm P LIN D w W JEE c pac T 550 0 600 0 Wavelength nm N dL m Nm XX FF re eio 1 IN oS olo Overall Spectral Power 103 248 mmm f 2l uum a J DI
15. ing gt J 333 Section 13 Returns and Repairs 397 Section 1 SAFETY INFORMATION A SAFETY WARNINGS Always observe the following safety precautions e The LumaSpec 800 is powered via its USB connection DO NOT attempt to connect the LumaSpec 800 to a power outlet This equipment is for use in moderate climates only NEVER use the equipment in damp or wet conditions Avoid excessive heat humidity dust amp vibration e Do not use where the equipment may be subjected to dripping or splashing liquids his equipment contains no user serviceable parts Refer all repairs to qualified service personnel e t is the user operators responsibility to ensure good laboratory practices are followed Section 2 PRODUCT OVERVIEW The LumaSpec Model LS800S is a compact visible light soectrometer which provides quantitative spectral power data microwatts per square centimeter per nanometer for microscope optical system illumination systems The LumaSpec has a range of 350 800 nanometers and a resolution of 1 5 nanometers In Irradiance Mode the information for the illumination system is displayed in both a graphical and numerical format on the LumaSpec main display Simply clicking on the graph provides spectral power data for the wavelength selected Ten selectable ranges are available which allow the user to view spectral power data at a glance for wavelength ranges of interest Integration time is quickly chan
16. ion It will be necessary to move the stage slightly so that you can view and focus on the edge of the aperture It is highly recommended to only move one axis when looking for the edge of the aperture and to move the stage and Illumination Target so that the light falls centrally on the aperture before taking measure ments This can be done iteratively by slowly moving the stage and maximizing the overall spectral power as viewed on the LumaSpec software or by looking under magnification and noting two edges of the aperture then moving the stage to the midpoint between the edges See Images below Objective Light is Off Center Page 15 Inverted Microscopes With Integrated Slide Holder Place a sample slide into the slide holder and bring the slide into focus Remove the sample slide and place the Illumination Target into the stage slide holder with the crosshair side UP Adjust the XY stage position until the aperture is located above the objective and the light from the CONDENSER falls centrally on the crosshair target At this point the Illumination Target is ready to use See image below Condenser Light is Off Center Light is On Center of Crosshair Inverted Microscopes Without Integrated Slide Holder Place a sample slide onto the stage and bring the slide into focus Remove the sample slide and place the Illumination Target into the stage slide holder with the crosshair side UP Adjust the XY stage
17. lation before connection the LumaSpec 800 to your computer Before installing the software please uninstall the LumaSpec software Omni driver software and VC 2010 Redistributable if the software was installed previously There is no need to uninstall NET Framework 4 0 if already installed on your computer Step 1 Double click on the Setup file in order to begin the installation process A command prompt window will open and the installation process will start Step 2 Install the latest NET Framework onto your computer If you already have the latest NET Framework installed you will see the window below If you get this window simply click Cancel and then click Yes to confirm i ae gU ic T x e Foa PUMA he d RJ M pa TM a o Y T G a dL MET Frare ur PF GIIETIE T TEN H Yran Le ITE it is ze mr ti a rm vini k z S pas x t il TA NET Framework 4 Client Profile Maintenance You can repair your installation or remove it From this computer Please select one of the Following options DJ ri i mier ee is ed inimi m is Pi rein rin is ieee m erha eee debe ses irt ine ris i ieee Pee Remove NET Framework 4 Client Profile From this computer Page 6 If you do not have the latest NET Framework installed you will receive the following window In this case you will need to proceed with the installation In order to proceed with the installation check the box to agree
18. lue rises over 14 500 Integration time is ideally set to maintain the maximum raw data value between 13 000 and 14 000 The image below is an example of a situation where the current integration time is set too high The red warning symbol in the lower right has appeared to indicate a lower integration time needs to be chosen The maximum raw data value is also greater than 14 000 which again confirms the integration time needs to be lower integrati 40 me Integration Time it too high UVOZA 550 0 600 0 650 0 Wavelength nm Maximum Raw Data KASA i 1 The sensor is saturated and a lower integration 15289 dh S Maximum Raw Data too high Section 11 GRAPH FUNCTIONS Scale Graph to Fill VVindovv Scales Y axis of display to enlarge graph in display Useful when overlaying multiple spectrums with widely varying spectral power PRIX Go ee sthentifit Tunt terga Pole graph to fill window gt gt f E a 5 3 4 k j j J J 5 7 1 r J 4 4 E Can t see entire spectrum uVv cm2 nm o e 550 0 600 0 Wavelength nm The green spectrum in the graph above is truncated The entire green spectrum is visible in the graph below after using the scale graph icon Entire spectrum visible E Ci c o a Page 31 Transmission Mode Allows the user to place a filter in the light path and determine the percent transmission in the wavelengths transmitted Useful for
19. maSpec software to connect to the LumaSpec Spectrometer If unit is connected to the PC upon start up the LumaSpec software will connect automatically Data Collection Setting Save Dark Spectrum Export to Excel Saves time stamped spectral power by wavelength in graphic and in numeric spreadsheet format Also saves integration time scans to average dark spectrum overall spectral power information and spectral power by range See sample of saved data below ew zero calibration Dark Spectrum for the LumaSpec _ Prior LumaSpec Microscope Spectrometer Wavelength nm 5 350 2471582 350 7033229 351 1595429 351 6158183 352 0721489 352 5285347 352 9849757 353 4414719 353 8980232 354 3546295 IntegrationTime Scan to Average Dark Spectrum Number of pixel in processed spectrum 945 Overall Spectral Power 70317 63413 Date Tuesday January 08 2013 Time 3 46 34 PM 0 475559395 0 266381955 0 310879859 0 278570821 0 360941355 0 338897747 0 385757396 350 2471582 365 7875629 381 3903857 397 0540133 412 7768327 428 5572306 444 3935939 460 2843094 476 227764 556 6796628 572 9056756 589 1731353 605 4804289 621 8259432 638 208065 654 6251811 671 0756785 687 5579439 704 0703641 720 6113261 737 1792167 753 7724226 770 3893308 787 0283281 0 467743515 0 245157668 0 504258562 Name Range 1 Range 2 Range 3 Range 4 Range 5 Range 6
20. ope focus mechanism to focus on the crosshair inscribed on the surface of the Illumination Target It is recommended to start with the lowest magnification objective when trying to find and focus on the crosshair Note that if white light is not available the coating on the Illumination Target will allow you to view and focus on the crosshair with many of the common fluorescent filter sets Carefully remove the Illumination Target and turn it over with the aperture side up At this point the Illumination Target is ready to use Inverted Microscopes Place a sample slide into the motorized stage slide holder and bring the slide into focus Remove the sample slide and place the Illumination Target into the stage slide holder with the crosshair side UP Adjust the XY stage position until the aperture is located above the objective and the light from the CONDENSER falls centrally on the crosshair target At this point the Illumination Target is ready to use See images below Condenser Light is Off Center Light is On Center of Crosshair Page 14 Setting Up the Illumination Target on a Manual Stage Upright Microscopes VVith Integrated Slide Holder Place the Illumination Target into the stage slide holder with the crosshair side UP Adjust the XY stage posi tion until the target is located below the objective and the light from the objective falls centrally on the crosshair target See images below P M E a E
21. position until the aperture is located above the objective and the light from the CONDENSER falls centrally on the crosshair target At this point the Illumination Target is ready to use See images below Condenser Light is Off Center Light is On Center of Crosshair Page 16 Section 7 INITIAL USE OF LUMASPEC SOFTVVARE Start the LumaSpec software Start Programs LumaSpec The main page for the LumaSpec program will appear and the following message will appear r i I emporarily displaying uncalibrated data adjust integration time unti Maximum raw data alue iz ideally between 13000 and 14000 counts Press OK A message pops up that allows you to choose to automatically set integration time The LumaSpec software is not able to automatically set some intense illumination sources and so if this is the first time using the LumaSpec software click on NO Manually setting Integration Time A window will pop up asking the user to Adjust the integration time until the maximum raw data value is between 13 000 and 14 000 or another acceptable value some light sources may be too dim to reach 13 000 but this would be a rare occurrence r Adjust Integration Time until maximum raw data value is between 1300 pi 4000 or another acceptable value then press O Integration Time 00 Ez Page 17 Manually Setting Integration Time Continued If the maximum raw data value is betw
22. ser to increase the minimum wavelength or decrease the maximum wavelength customizing the graph on the main display to show only the spectral information of interest to the user Clicking on the apply button allovvs the user to evaluate the change before closing the tools menu Default Wavelength Min 400 i nmi Save Settings Open Settings Data Collection Settings The LumaSpec software allows the user to perform real time spectral data collection The frequency dura tion and start delay for the data collection function are adjusted in the tools menu The acceptable range for each parameter frequency duration and start delay is 0 999 Values can be input directly or increased and decreased using the up down arrows Available units are in milliseconds seconds minutes and hours Frequency Duration F Seconds x ms Open Save Settings Settings connect Use Initial Dark Spectrum Checking the use initial dark spectrum box eliminates the need to save a new dark spectrum zero calibration each time the integration time is changed Default state is unchecked Tools Data Collection Settings Scans to Average ff Default Wavelength Use Initial Dark Spectrum Open Settings Clicking on this button allows the user to recall previous settings for range selection data collection settings scans to average The user must have previousl
23. system of your computer If you have both the x64 and x86 version installed uninstall both programs OmnibDriver redistributable win64 2 20 This program may be installed as the win32 version depending on the operating system of your computer Restart the computer Go through the installation process using the CD provided with the spectrometer Restart your computer after the installation has completed Page 34 Issue The spectrum graph is not smooth very noisy signal Noisy Signal Suggestion Increase the integration time until the Maximum Raw Data Value is between 13000 and 14000 counts Issue The spectrum graph is displaying 100 output only not the proper spectrum Click to Return to Spectrum Transmessioni 26 Line 100 Suggestion Click on the Irradiance Icon uj to change display from Transmission Mode to Irradiance Mode Page 35 Issue Want to adjust the Y axis range manually Suggestion Right click on the graph and select Change Chart Y Axis Value The AutoScale Window will pop up and allow the user to choose a custom spectral power range Issue The main display is blank or only shows a small portion of the graph negaton Tune GODO users Click Here to See Entire Graph dA nd f Bre O G 18i 6 85 4 Not Seeing Entire Graph Wavelength inm E Suggestion Click on the circles with the hyphen in the center on the left side of the graph to view the entire
24. this computer Repair Microsoft Visual C 2010 x64 Redistributable to its original state Remove Microsoft Visual C 2010 x64 Redistributable from this computer Yes send information about my setup experiences to Microsoft Corporation For more information read the Dats Collection Policy If you do not have the C 2010 x64 Redistributable installed you will receive the window below Agree to the Microsoft terms and conditions and click Install to begin the installation process Welcome to Microsoft Visual C 2010 64 Redistributable Setup Please accept the license terms to continue Yes send information about my setup experiences to Microsoft Corporation For more information read the Dats Colection Policy Once the installation has completed click Finish The installation process is now complete and your software is ready to use Installation Is Complete Microsoft Visual C 4 2010 x64 Redistributable has been You can check for more recent versions of this package on Connecting the Fiber Optic Cable Connect the fiber optic cable part number VV4221 to the LumaSpec Box Connect the other end of the fiber optic cable to the Illumination Target as show below Tighten both connections hand tight only Do not use any tools when connecting the fiber optic cable Fiber optic cable connected to the Illumination Target VV4221 fiber op
25. tic cable properly connected to the LumaSpec box and Illumination Target Connecting the USB Cable Connect the micro USB connector to the LumaSpec Box and the opposite USB connector to your computer If you do not have the latest NET Framevvork installed you vvill receive the follovving vvindovv In this case you will need to proceed with the installation In order to proceed with the installation check the box to agree to the Microsoft terms and conditions and click Install Step 3 Install the LumaSpec software Click Next on the first window that opens in order to begin the installation Setting up the Illumination Target Placing the Illumination Target aperture centrally on the optical axis of the microscope stage and at the correct focus height is critical to obtaining repeatable spectral power readings from your illumination source The Illumination Target shown below has two sides the crosshair side and the aperture side Aperture Side Crosshair Side Page 13 Setting Up the Illumination Target on a Motorized Stage Upright Microscopes Place the Illumination Target into the stage slide holder with the crosshair side UP Adjust the XY stage position until the target is located below the objective and the light from the objective falls centrally on the crosshair target See images below Objective Light is Off Center Light is On Center of Crosshair Use the microsc
26. to the Microsoft terms and conditions and click Install HET Framework 4 Client Profile Setup Please accept the license terms to continue E 2 r E ge TPE A I I ee ore MM 4 SET T r pd ki E da aj igi E d Installation Is Complete NET Framework 4 Client Profile has been installed Check for more recent versions on Windows Update Page 7 Step 3 Install the LumaSpec software Click Next on the first window that opens in order to begin the installation Welcome to the LumaSpec Setup Wizard The installer vall guide you through the steps required to install Luma pec on your computer WARNING This compuler program is protected by copyright law and intemational treaties Unauthorized duplication ot distibution of this program ar any portion of i may result in severe civil of Giminal penalties and will be prosecuted to the maximum extent possible under the law On the next window select whether you would like to install the program for all users on the computer or just the current user by selecting Everyone or Just Me and then click Next In this window you can also change the folder in which the program will be installed if you would like it to be a location other than the default Prior recommends you do not change the default location unless it is a requirement for your system EEE H Bru 2 we s I um f E rdum gt
27. y used save settings button wu IM Desktop b gt Search Desktop Organize v New folder E LM e Data Collection Settings ans to Average i N Favorites Libraries mE Defa n Pi E EE Desktop R de 9 i 8 Downloads x a a Libraries s ndaj lt Kon Open Save Documents Settings Settings T J Music l i Pictures Netyvork H Videos M System Folde Computer i Sy OS C ew USB DISK E File name LumaSpec Test xls M Settings File Y Open M Cancel j X JAnnn RR n RNN n Save Settings Allows the user to save current settings for range selection data collection settings scans to average These settings can be recalled for later use See Open Settings B ES i ee dini ni Save As Va Y m Desktop v ty Search Desktop Organize New folder E kd s Da on Settings Scans to Average n E 2 Li I 2 Favorites Libraries c Default Wavelength Deskto System Folde T Downloads Max A I 4 3 Libraries aa Initial Dark Grant T P Computer Open Save Jse Initial Dark Spectrum Documents gt System Folde Settings Settings T a Music s Pictures Ww SN E IM Videos System Folde i 4 j Computer File na TU Save as type 5e a Hide Folders Save Cancel 00 0 550 0 600 0 Connect Allows the Lu
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