Transferpette Multichannel Electronic User Manual 2003
Contents
1. In order to aspirate a larger volume than was set up to a max of 110 of the nominal volume press and hold the pipetting key until the desired volume has been aspirated The display shows a rhombus press and hold What to do 6 Discharge liquid in the GEL mode 7 Initiate blow out 8 End pipetting Note Electrophoresis GEL Mode How to do it Keys to press Display readout Press the pipetting key once to discharge the liquid The rhombus is shown in the display The liquid is discharged very slowly Interrupt discharging To interrupt discharging press the pipetting key again The display shows the volume discharged prior to interruption Press the ENTER key after the last pipetting operation The display shows blo for blow out Press the pipetting key once to initiate the blow out process The residual liquid is discharged After the residual liquid is discharged blow out the display moves back to the start position The GEL mode operates using a very slow discharge speed to prevent swirling of the samples To assure optimal discharging into a gel this discharge speed is fixed for the GEL mode This speed is significantly slower than level 1 and cannot be selected individually DISP Mode The Programs Settings and Use Program for discharging an aspirated liquid in pre defined steps The volume aspirated will be a little bit more than actually needed Speed adj2. 0 2 0 02 207C Ex Type of tips ul 300 200 300 20 Adjustment of the Transferpette 8 12 electronic Easy Calibration The calibration mode CAL The instrument should be set to either the nominal volume for example 100 ul for a 100 ul pipette or a specific test volume in the standard pipetting mode PIP See page 40 for procedures What to do How to do it Keys to press Display readout 1 Bring up the CAL Press and hold the MENU mode key gt 3 sec until CAL 1x q appears The display gt 3s J reads off CAL blinks i 2 Activate the CAL Press one of the arrow mode buttons to activate the CAL mode The display changes from off to on CAL continues to blink lt gt 3 Confirm CAL mode Press the ENTER key The display now shows the set pipetting volume CAL blinks 4 Set the volume Use the arrow keys to set the volume which was previously determined and tested CAL blinks 5 Confirm volume Press the ENTER key The display shows the tested and corrected volume The CAL symbol is continously displayed to confirm that an adjustment has been made Revert to factory default settings The continually displayed CAL symbol refers to a previously made adjustment What to do 1 Bring up the CAL mode How to do it Press and hold the MENU key gt 3 sec until CAL appears The display reads Easy Calibration Keys to pres
3. Liquid Handling Easy Handling ry BRAND electronic B RAN D FIRST CLAS ED Gebrauchsanleitung N HED Operating Manual Fe Mode d emploi BET Instrucciones de manejo EG Konformit tserkl rung EC Conformity Declaration Das bezeichnete Ger t entspricht den einschl gigen grundlegenden Anforderungen der aufgef hrten EG Richtlinien und Normen Bei einer nicht mit uns abgestimmten nderung des Ger tes verliert die Erkl rung ihre G ltigkeit The deviced named below fulfills the relevant fundamental requirements of the EC directives and standards listed In the case of unauthorized modifications to the device the declaration becomes invalid Ger tebezeichnung Device name Transferpette electronic Ger tetyp Device type mikroprozessorgesteuerte akkubetriebene Kolbenhubpipette nach dem Luftpolsterprinzip microprocessor controlled battery operated air interface pipette Hersteller Manufacturers BRAND GM BH Co KG Adresse Adress Otto Schott Str 25 D 97877 Wertheim Einschl gige EG Richtlinien Normen Relevant EC directives standards E 89 336 EWG EN 61326 1997 A1 1998 A2 2001 oO 73 23 EWG EN 61010 1 2001 EN 60950 2000 Safety Instructions Functions and Limitations of Use Operating Exclusions Operating Elements The First Steps Setting the Volume Setting the Aspiration and Discharging Speed Correct Pipetting The Pipetting Programs PIP mode PIPmix mode revPIP m
4. separate Fig A the piston nut and screw off the piston nut Fig B pull out the piston Fig C piston and screw on the piston nut and tighten with the mounting tool Fig D check the instrument for tightness s page 59 Servicing Remove the piston nut and the Insert the cleaned or new piston piston and tighten the piston nut Charging and Replacing the Battery A fully charged battery allows approx eight hours of continuous pipetting of liquids with a viscosity and density similar to water Important Before charging the battery ensure that the AC adapter is compatible with the line voltage in the labora tory Do not charge the device in an explosive environment The battery can only be charged inside the Transferpette 8 12 electronic Charge the battery a Insert the charging cable plug for the AC adapter into the jack at the top of the Transferpette 8 12 electronic charging starts automatically b During the charging the bars for the battery capacity run continually from the bottom to the top The battery is fully charged when the bars in the display have stopped moving Pipetting during charging During charging you can continue to work with the Transferpette 8 12 electronic If the battery is fully discharged it will take a few minutes until a certain minimum charge capacity is available which is needed to operate the instrument safely Note The last settings are store
5. 2 Turn both closures of the manifold housing cover 90 and slide off the housing 3 Push the mounting tool on the nose cone and unscrew it Fig A and B 4 Push the piston unit down to the bottom Remove the seal and inspect it and clean or replace if necessary Fig C Note The seal Abb C will either remain inside the nose cone or will stay on the piston after the nose cone is removed 6 1 required lubricate the piston lightly with the supplied silicone oil 7 Push the seal on the piston with its flat side facing up ward Use the mounting tool to mount and tighten the cleaned or new nose cone Fig D 8 Reassemble the manifold and check the instrument for tightness s page 59 oervicing Remove the seal for cleaning Insert the seal and screw in or replacement the cleaned or new nose cone Removing of pistons for cleaning or replacing 1 2 Remove both outer Phillips Pull the piston and nose Place the mounting tool on Remove the piston nut and Insert the cleaned or new Reassemble the manifold and Disassembling the Manifold Unscrew the manifold from the handle Turn both closures of the manifold housing cover 90 and slide off the housing screws on the nose cone assembly Attention Separate the piston and nose Screw off the piston nut cone assembly Do not loosen the central guide rod Z cone assembly apart and
6. sec During the discharging process the capacity bars for the battery 1x gt 3s indicator run continually from the top to the bottom YY c After the controlled discharge up to 3 hours the charging process 3 5 hours is started automatically During charging the capacity bars run continually from the bottom to the top Interrupting the discharge charge cycle Press any button to end the program The instrument switches automatically to the standard pipette mode PIP and to the nominal volume and the normal charging process is started automatically see page 62 Removing the plug for the AC adapter also ends the program Troubleshooting If an error occurs the instrument display shows Err and the error number is also shown The instrument will now only react to the ENTER key Pressing the ENTER key will attempt to restart the instrument Therefore a reference run is automatically requested Problem Instrument does not react Instrument does not react Instrument does not react Instrument does not react Display is dark Tip drips instrument not sealed or volume error Liquid is not aspirated or there is a loud motor noise Error message Possible cause Battery discharged or faulty Faulty electronic component Faulty electronic component Unpredicted program error No battery inserted Battery is defective Faulty electronic component Electrostatic discharge Im
7. 45 place the tips against the vessel wall Press the pipetting key again and the liquid is com rai pletely discharged including j J automatic blowout After wards wipe pipette tips cy against the vessel wall 4 Eject tips Hold the pipette shaft over a suitable disposal con ae tainer and press the tip Tip ejection ejection key key lt U4 The Pipetting Programs Page Normal Pipetting PIP Mode 44 Standard program A previously set volume is aspirated into the pipette tips and then discharged Mixing of Samples PIPMIX Mode 46 Program for mixing liquids The sample is repeatedly aspirated and discharged Reverse Pipetting revPIP Mode 48 Program especially for pipetting liquids with a high viscosity or vapor pressure or that tend to foam Pipetting for Electrophoresis GEL Mode 50 Program for loading electrophoresis gels A predefined sample volume is aspirated at high adjustable speed and then slowly discharged J Dispensing DISP Mode 52 Program for dispensing liquids An aspirated volume is dispensed repeatedly in defined steps PIP Mode The Programs Settings and Use The standard program a previously set volume is aspirated and then discharged Volume and speed adjustments are described on pages 40 and 41 What to do How to do it Keys to press Display readout times to bring up the mode selection menu Mode blinks 1 Bring up the men
8. d easily What to do How to do it Keys to press Display readout 1 Activate volume Press one of the arrow A setting keys to activate volume selection VOL blinks Vv 2 Change the volume Reduce volume Press the down arrow key to reduce the volume Holding the arrow key wY down accellerates the rate 2 of change VOL continues to blink PIP Increase volume Press the up arrow key to increase the volume Holding the arrow key down accellerates the rate of change VOL continues to blink lt D 3 Confirm volume Press the ENTER key setting The display now shows the new volume setting in this case 102 8 ul in the PIP mode Important By pressing the MENU key any procedure can be cancelled The display then moves to the next setting or back to the initial display depending on actual selection Setting the Aspiration and Discharging Speed The aspiration and discharging speeds can be individually adjusted When the menu is called up the last speed setting is shown Five speed levels are available What to do How to do it Keys to press Display readout Setting the aspiration speed 1 Bring up the menu Press the MENU key once to bring up the aspiration speed menu Speed blinks 2 Change the Press one of the arrow aspiration speed keys to select the desired speed in this case level 5 Speed continues to blink 3 Confirm speed Press the ENTER key The level d
9. d in the memory of the instrument If the battery is fully discharged or the battery is changed these settings are saved Replace the battery a Open the battery compartment cover Remove the battery and pull the plug gently out of the socket b Insert the plug of the new battery into the socket and insert the battery c Put the battery compartment cover in place again and close it Remove the battery from the instrument when it is not to be used for longer periods Charging and Replacing the Battery Battery display after a After the battery is inserted the display inserting a battery shows the full capacity indicator with a blinking frame the instrument does not recognize the charging status right now After 3 5 hours of charging time safe full charging of the battery the frame stops blinking Note After inserting a battery always charge 3 5 hours Battery regeneration function Refresh function In order to extend the service life and to optimize performance of the battery the Transferpette 8 12 electronic has a regeneration function refresh function This program provides a controlled full discharge and recharging of the battery To optimize the battery performance this refresh function should be used periodically Perform the refresh a Insert the plug for the AC adapter into the function jack on the top of the Transferpette 8 12 electronic b Press and hold the lower arrow key gt 3
10. d into the tips J Press the pipetting key i again and the volume is discharged again and so on 8 Initiate blow out Press the ENTER key after the last pipetting operation j 1x The display shows blo for J blow out i Press the pipetting key once to initiate the blow 7 A rev PIP m Th idual out process The residua J i 1x 2 liquid is discharged by h Qi 9 End pipetting After the residual liquid is discharged blow out the display moves back to the start position Electrophoresis GEL Mode The Programs Settings and Use Program for loading electrophoresis gels A predefined sample volume is aspirated into the pipette tips with high adjustable speed and then slowly discharged Volume and speed adjustment is described on pages 40 and 41 What to do How to do it Keys to press Display readout 1 Bring up the menu Press the MENU key three times to bring up the mode selection menu Mode blinks 2 Select GEL mode Scroll through the modes using the arrow keys until GEL appears Mode continues to blink 3 Confirm GEL mode Press the ENTER key The Display now shows plo for blow out 4 Prepare for Press the pipetting key pipetting once to move the pistons into the start position The arrow in the display points upwards aspiration 5 Aspirate liquid Press the pipetting key once The set volume is aspirated into the tips Aspirate a larger volume
11. e the troubleshooting guide on page 64 Inspect nose cones and seals for damage Inspect the pistons and seals for contamination and damage Servicing and Cleaning individual nose cones is described in detail in the instructions enclosed with the spare part Piston unit with piston support bar and pistons inserted in this unit which can be screwed out indivi dually for cleaning or replacement Nose cone assembly with nose cone support bar II and central guide rod Z which is attached to this and the nose cones and seals which can be screwed out individually for cleaning or replacement Manifold housing which is connected to the manifold housing cover III of the piston unit with two turn lock fasteners Cleaning Instructions 1 Let these parts dry and cool down completely Residual Lubricate the piston with a very thin coating of the Clean single nose cones pistons and nose cone support bar piston support bar these components only with soap solution or isopropyl alcohol afterwards rince with distilled water moisture in the nose cones may result in a loss of accuracy silicone oil supplied For the central guide rod Z only use the recommended fluorstatic grease After assembling the device performe the reference run rEF Disassembling the Manifold Removing of nose cones and seals for cleaning or replacing 1 Unscrew the manifold from the handle
12. ff 10 min after the last instrument operation Auto Power Off The First Steps Is everything in the package Confirm that your package includes Transferpette 8 or 12 electronic pipette battery power supply unit with battery charging cable 1 Tip Box SL filled 1 Tip Rack for refill 1 instrument stand 1 reagent reservoir silicone oil operating manual and 1 set of V rings made of Viton Initializing the Transferpette 8 12 electronic 1 Insert the a Open the cover of the battery compartment battery b Insure that the plug for the battery is firmly connected to the pipette Insert the battery c Replace the battery compartment 2 Activate the The Transferpette 8 12 instrument electronic automatically requests a reference run directly after the battery is inserted After the pipetting key is pressed the reference run is carried out and the instrument is now ready for pipetting The display shows the Pipetting mode standard factory setting pipetting mode PIP Battery charge and the nominal volume indicator for example 100 0 ul Default aspiration and dis Up arrow charging speeds are at maxi indicating mum The adjustment of aspiration volume and speed is de scribed on the following Volume setting pages Setting the Volume The volume for the Transferpette 8 12 electronic is set at the factory to the nominal volume of the instrument and can be changed quickly an
13. ge 37 Observe all safety precautions provided by reagent manufacturers Only pipette liquids that do not react with polypropylene PP and polycarbonate polybutyleneterephthalate PC PBT 6 Never use force on the instrument 7 Operate only with tips attached see page 42 Do not lay the instrument down horizontally when the tips are attached Avoid touching the tip orifices when working with hazardous samples Always work in a way which neither endangers the user nor any other person When emptying the tips the sample must never spray or splatter into the surrounding area Discharge into suitable collecting vessel 10 11 12 13 14 15 16 17 Only press the pipetting key after ensuring that discharging the sample will not result in any danger Only dispense liquids into a suitable collecting vessel Do not use the instrument in potentially explosive environments or with explosive samples Only use AC adapter indoors Protect from moisture To charge the NiMH battery pack use only the original AC adapter Do not replace the original battery pack with other battery packs or cells Use only original manufacturer s replacement battery packs Contirm battery pack is completely discharged prior to disposal Observe relevant disposal regulations for your facility Use only original manufacturer s accessories and spare parts Do not modify the instrument in any wa
14. h the pipetted quantity with an analytical balance Please follow the operating manual instructions from the balance manufacturer d Calculate the volume taking the temperature into account e 3 10 pipettings and weighings per channel in three volume ranges 100 50 10 are recommended for statistical analysis Calculation for nominal volume x Weighing results Z Correction factor n Number of weighings for example 1 0029 ul mg at 20 C 1013 hPa 3 em ee Mean value x Mean volume V X Z Accuracy Standard Deviation Coefficient of Variation vi A T A 2 100 s er cv 100s Vo n 1 v V Nominal volume 0 Calculation of accuracy A and variation coefficient CV A and CV are calculated according to the formulas for statistical control Final test values related to the nominal capacity maximum volume indi cated on the instrument obtained when instrument and distilled water are equilibrated at ambient temperature 20 C 68 F and with smooth operation According to DIN EN ISO 8655 Accuracy tolerances for the Transferpette 8 12 electronic Volume range Volume step ul ul 30 300 300 150 30 10 100 100 50 10 2 20 20 10 2 A lt 0 6 1 2 3 0 0 8 1 6 4 0 1 0 2 0 8 0 A Accuracy CV Coefficient of Variation cV lt 0 25 0 4 1 2 0 25 0 4 1 5 0 5 1 0 3 0 Accuracy Table Fl Increment ul 0 2
15. ischarge of the residual liquid blow out the display moves back to the start position Note The display shows a maximum of 19 cycles The Programs Settings and Use revPIP Mode Program for pipetting of liquids with high viscosity vapor pressure or that tend to foam Volume and speed adjustments are described on pages 40 and 41 What to do Display readout How to do it Keys to press 1 Bring up the menu Press the MENU key three Select revPIP mode Confirm revPIP mode Prepare for pipetting Aspirate liquid 1 i N Discharge liquid in the revPIP mode times to bring up the mode selection menu Mode blinks Scroll through the modes using the arrow keys until revPIP appears Mode continues to blink Press the ENTER key The Display now shows plo for blow out Press the pipetting key once to move the pistons into the start position The arrow in the display points upwards aspiration Press the pipetting key once The volume aspirated will be a little bit more than set To discharge the measured amount of liquid press the pipetting key once The arrow in the display points downwards discharge Some liquid will remain in the tips Er TE rev PIP revPIPMode What to do How to do it Keys to press Display readout 7 Repeat aspiration Press the pipetting key of liquid in revPIP again and the set volume j mode is aspirate
16. isplay returns to the start position for the current pipetting mode in this case the standard PIP mode Setting the discharging speed 1 Bring up the menu Press the MENU key twice to bring up the discharging speed menu Speed blinks 2 Change the Press one of the arrow discharging speed keys to select the desired speed in this case level 2 Speed continues to blink 3 Confirm speed Press the ENTER key The level display returns to the start position for the current pipetting mode in this case the standard PIP mode with the Transferpette 8 12 electronic Correct Pipetting The volume is set at the factory to the nominal volume for the Transferpette 8 12 electronic and can be changed quickly and easily See page 40 Quick start in the standard pipetting mode 1 Attach the tips Note Ensure the tips are seated firmly and seal against the nose cones 2 Aspirate liquid Hold the pipette vertically and immerse the tips 2 to 3 mm into the liquid Press the pipetting key to aspirate the liquid into B the tips The arrow in the J display points upwards to indicate the aspiration of gt liquid 1x Note To avoid the intake of air leave the tips immersed into the liquid for approx 1 sec 3 Discharge liquid After the liquid has been aspirated the arrow in the display points downwards to indicate discharging Hold the pipette at an angle between 30 and
17. ment for pipetting liquids that react adversely with polypropylene PP shaft or polycarbonate polybutyleneterephthalate PC PBT handgrip Avoid reactive vapors due to the danger of corrosion Do not use use aggressive cleaning agents e g bleach to clean the instrument Distilled water or a mild soap solution is recommended The pipetting of highly dense or viscous liquids as well as the pipetting of liquids which react with polypropylene PP or polycarbonate polybutylene terephthalate PC PBT is only possible within limitations Operating Elements The Transferpette 8 12 electronic is a microprocessor controlled battery operated piston stroke multichannel pipette which has been optimized for ergonomic opera tion and ease of use Charging jack Far ISA Der Display fa fi oa Program keys x compartment Pipetting lt Tip ejection key The Transferpette 8 12 electronic rests easily and ergonomically in your hand The finger rest is height adjustable with a screw which allows fine tuning of the hand position for effortless operation of the function buttons Handgrip Manifold Key Functions Up key Entry N confirmation an Power On Down key Pipetting key The instrument is activated by pressing the ENTER key After pressing the pipetting key next the instrument is ready for pipetting The Transferpette 8 12 electronic shuts o
18. ntained at their lowest position to avert an accidental aspiration of liquid When the key is released the pistons return to the start position PIPmix Mode The Programs Settings and Use Program for mixing of liquids The sample is repeatedly aspirated and discharged Volume and speed adjustments are described on pages 40 and 41 What to do How to do it Keys to press Display readout 1 Bring up the menu Press the MENU key three times to bring up the mode selection menu Mode blinks 2 Select PIPmix Scroll through the modes mode using the arrow keys PIP mix until PIPmix appears y Mode continues to blink 3 Confirm PIPmix Press the ENTER key mode The Display now shows blo for blow out 4 Prepare for Press the pipetting key pipetting once to move the pistons into the start position The arrow in the display points upwards aspiration 5 Aspirate liquid Press the pipetting key once to aspirate the liquid PIPmix Mode What to do How to do it Keys to press Display readout 6 Discharge liquid Press and hold the pipetting y in the PIPmix key and the liquid is mode alternately aspirated and J discharged The display shows the up arrow for a aspiration and the down p arrow for discharging and press and hold the number of cycles End pipetting Press the pipetting key once and the liquid is discharged and the blow out function initiated After the d
19. ode Electrophoresis GEL mode DISP mode Checking the Volume Accuracy Table Easy Calibration adjustment Autoclaving Reference Run rEF Servicing and Cleaning Disassembling the Manifold Charging and Replacing the Battery Battery Regeneration Function Troubleshooting Ordering Information Accessories Spare Parts Repairs Page 36 37 37 38 39 40 41 42 43 44 46 48 50 52 54 55 56 58 58 59 60 62 63 64 65 66 Safety Instructions This instrument may sometimes be used with hazardous materials operations and equipment It is beyond the scope of this manual to address all of the safety problems associated with its use in such applications It is the responsibility of the user of this pipette to consult and establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use IN Please read the following carefully 1 Every user must read and understand this operating manual prior to using the pipette and observe these instructions during use Follow general instructions for hazard prevention and safety instructions e g wear protective clothing goggles and gloves When pipetting infectious or other hazardous samples all appropriate regulations and precautions must be followed Only use the instrument for pipetting only liquids that conform to the specifications detined in the operating exclusions and limitations see pa
20. proper tip Tip is not properly seated Piston seal is contami nated or damaged Piston movement is limited Corrective action Charge battery for at least 5 min without operating then only operate with charging cable attached until battery is recharged Replace battery if needed Send in the instrument for repair Send in the instrument for repair Confirm error by pressing the ENTER key The instrument is reinitialized Insert battery Replace battery Send in the instrument for repair Remove and insert the battery Only use quality tips Press tip in firmly Clean the instrument replace the seal Unscrew the manifold and move the metal part in the middle by hand Ordering Information Accessories Spare Parts Transferpette 8 electronic incl AC adapter 1 10V 50 60 Hz Volume 2 20 ul 10 100 ul 30 300 ul Cat No 27054 02 27054 08 27054 12 Transferpette 12 electronic incl AC adapter 1 10V 50 60 Hz Volume 2 20 ul 10 100 ul 30 300 ul Cat No 27054 22 27054 28 27054 32 AC adapters Quality pipette tips PLASTIBRAND Cat No racked in Tip Box SL 7053 59 Volume Total tips per pack Cat No 0 1 20ul 96 102202 0 5 20 ul 96 7022 04 2 200 ul 96 7022 06 5 300 ul 96 7022 08 Repl battery Silicone oil Fluorstatic gr Spare parts Transferpette 8 12 electronic Cat No Cat No Cat No Parts will differ slightly depending on nominal 7055 00 7036 77 7036 78 volume of inst
21. rument Fig shows spare parts for Transferpette 8 12 electronic 10 100 ul Volume A B C D 2 20 ul 7056 70 7056 80 7034 13 7033 41 10 100 ul 7056 72 7056 82 7034 91 7033 44 Piston Nose ne ao 30 300 ul 7086 74 7056 84 7034 91 7033 46 Repairs If a problem cannot be fixed by following the troubleshooting guide or by replacing one of the parts listed on page 65 then the instrument must be sent in for repair Please note for the safety of courriers and our employees and to avoid violation of federal and local laws only clean instruments free of any chemical biological or radioactive hazards can be inspected and repaired Sending in for repair Important Transporting of hazardous materials without a permit ist a violation of federal law BrandTech Scientific Inc will not accept instruments that are not appropriately cleaned and decontaminated Therefore contact BrandTech Scientific Inc and obtain return authorization before sending your instrument for service Note No warranty claims can be considered if repairs other than those described have been made or attempted
22. s 1x q j gt 3s J on CAL blinks 2 Deactivate CAL mode Press one of the arrow keys to deactivate the CAL mode The display changes from on to off CAL continues to blink lt b gt 3 Revert to factory setting Press the ENTER key The CAL symbol disappears The instrument has now j been reverted to factory i default setting 1x Important When the Transferpette 8 12 electronic is adjusted a volume offset is performed which means that the volume is changed across the entire volume range of the pipette by the same amount It is recommend that the adjustment be performed at 50 of the nominal volume Note The instrument is permanently adjusted for watery solutions but it can also be set for solutions with varying density viscosity and temperature The Transferpette 8 12 electronic can be adjusted in every mode with the exception of the GEL mode Autoclaving The manifold M of the Transferpette 8 12 electronic highlighted in picture can be autoclaved at 121 C ata pressure of 2 bar for 20 minutes according to DIN Note The handgrip can not be autoclaved Eject the pipette tips Unscrew the manifold from the grip Autoclave the complete manifold without any further disassembling Allow the manifold to completely cool and dry Screw the manifold into the grip O 91 gt 0 N Perform a reference run rEF Note The effec
23. tiveness of the autoclaving must be verified by the user If the manifold is autoclaved frequently then the pistons should be lubricate with the supplied silicone oil in order to provide smoother movement Reference run rEF A manual reference run must be completed each time the manifold is reattached to the handle The reference run is needed to assure secure connection of the pistons What to do How to do it Keys to press Display readout 1 Bring up rEF mode Simoultaneously press the pe MENU and the ENTER key g a to activate the rEF mode 2 Perform the Press the pipetting key reference run once to start the reference run A noise can be heard clearly indicating the function is being performed Note After the reference run the display automatically returns to the previous program The three main components of the manifold can be easily separated and disassembled for servicing cleaning or replacing parts The procedures are illustrated on the following pages In order to assure proper functioning the Transferpette 8 12 electronic should be serviced and cleaned at regular intervals What is to be inspected 1 2 Note The changing of the V rings O rings on the The main components of the manifold A B Servicing Test the sealing of the instrument To do this aspirate a sample and then hold the instrument in a vertical position for about 10 sec If a drop forms at the tip orifices se
24. u Press the MENU key three 3X q 3 A A rev PIP f m j J T 2 Select PIP mode Use one of the arrow keys to scroll through the modes until PIP appears Mode continues to blink 3 Confirm PIP mode Press the ENTER key The display now shows blo for blow out 4 Prepare for Press the pipetting key pipetting once to move the pistons into the start position The arrow in the display points upwards aspira tion 5 Aspirate liquid Press the pipetting key once to aspirate the liquid What to do 6 Discharge liquid r Start blow out How to do it Press the pipetting key once to discharge the liquid The arrow in the display points downwards discharge No action required When pipetting in the PIP mode the blow out function is performed automatically Start blow out manually The blow out function can if necessary be initiated manually at any time 1 Bring up the blow out function 2 Start blow out Note Press the ENTER key The display shows blo for blow out Press the pipetting key once to initiate the blow out process The display moves back to the start position of the selected pipetting mode Keys to press PIP Mode Display readout To accomplish the blow out the pistons move to the lowest position The user must be certain that any residual liquid is discharged safely If the pipetting key is pressed and held the pistons will be mai
25. uid yt DISP steps L i rir AYA LOL ua 10 Dispense Each time the pipetting key liquid is pressed one dispensing A 1 step is performed The DISP stops arrow in the display points i vy bare downwards discharge The r eK ua display shows the number of dispensing steps left 11 Initiate blow out Press the ENTER key after Ez the last dispensing step 1x The display shows blo j 3 for blow out Press the i pipetting key next once to initiate the blow out process see also p 51 12 End dispensing After the residual liquid is discharged blow out the A me display moves back to the DISP steps I start position N frag ANA ALt o Checking the Volume Depending on use we recommend inspection of the instrument every 3 to 12 months The cycle can however be adjusted to individual requirements The gravimetric testing of the pipette volume is performed according to the following steps and is in accordance with DIN EN ISO 8655 Part 6 1 Set nominal volume See page 40 for procedure 2 Condition the pipette Condition the pipette before testing by using pipette tips to aspirate and discharge the test liquid distilled H O five times After this discard the pipette tips 3 Carry out the test a Attach new pipette tips and pre rinse one time with test liquid b Aspirate liquid and pipette it into the weighing vessel Note Each individual channel must be tested separately c Weig
26. ustment is described on page 41 What to do 1 Bring up the menu 2 Select DISP mode 3 Confirm DISP mode 4 Prepare for dispensing 5 Set dispensing step volume 6 Confirm dispensing step volume How to do it Press the MENU key three times to bring ap the mode selection menu Mode blinks Scroll through the modes using the arrow keys until DISP appears Mode continues to blink Press the ENTER key The Display now shows blo for blow out Press the pipetting key once to move the pistons into the start position The arrow in the display points upwards aspiration Press the arrow keys to set the volume Holding the arrow key down accellerates the rate of change VOL blinks Press the ENTER key The display now shows the new volume setting for the dispensing steps and the max number of steps Steps blinks TE Keys to press Display readout wi lt D 071 DISP steps L 7171 LI ut D x ii DISP Mode What to do How to do it Keys to press Display readout Set the number Press the arrow keys A of steps to set the number of steps a i Steps continues to blink 7 j DISP steps I 3 11171 L006 ut 8 Confirm the Press the ENTER key number of steps The display now shows the j 1 number of steps that has j DISP steps I been set fili Li u 9 Aspirate Press the pipetting key liquid once to aspirate the liq
27. y other than specifically described by the manufacturer In case of malfunction immediately stop pipetting Consult the Troubleshooting section of this manual and contact the manufacturer if needed CZD Improper use of the instrument or the batteries short circuit mechanical damage overheating incorrect AC adapter etc can lead to battery explosion Functions and Limitations of Use The Transferpette 8 12 electronic is a micro processor controlled battery operated piston stroke multichannel pipette which uses the air displacement principle for the pipetting of aqueous solutions with an average density and viscosity Limitations of use The Transferpette 8 12 electronic is intended for the pipetting of liquids within the following limitations Temperature of both the instrument and solution should be between 15 C to 40 C 59 F to 104 F for the instrument and the reagent Consult the manufacturer for use in tempera tures outside of this range Vapor pressure up to 500 mbar Viscosity 260 mPa s 260 cps Battery and power supply unit specifications Battery Nickel metal hydride battery 3 cylindrical individual cells with size AAA 3 6 V 700 mAh Power supply unit Output voltage 6 5 V DC 200 mA When the instrument is used properly the sample only comes into contact with the tips and not with the Transferpette 8 12 electronic Operating exclusions Never use the instru
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