BioLogic LP low pressure liquid chromatography system
Contents
1. STORE UP TO 50 METHODS e ALPHANUMERIC METHODS NAMING PROTOCOL e CONTROL OF UP TO 5 BUFFERS TIME OR VOLUME BASED PROGRAMMING e IN PROGRESS METHODS EDITING e ONLINE CONTEXT SENSITIVE HELP FUNCTION e AUTOMATIC SENSING OF VALVE PRESENCE e FLOW RATES TO 40 ML PER MINUTE e PROGRAM UP TO 50 COLLECTION WINDOWS e THREE PROGRAMMABLE ALARMS AND HOLD OPTION e CONDUCTIVITY MONITORING e STANDARD 280 NM AND 254 NM UV FILTERS e CHOICE OF BIO RAD 2110 OR 2128 COLLECTORS Sr a El HER w ana oo O Single keypad control of all instruments LED status light lets you know which component is operating Online context sensitive Help offers immediate answers to questions Selectable modes of operation Instrument keys and icons address instruments independentiy 7 Running the BioLogic LP System is an almost effortless task The front control panel offers a logical layout as well as instrument keys with icons that are identifiable at a glance And once you ve actually tried the system programming quickly becomes second nature One of the many advantages of the BioLogic LP System is the ability to edit methods in progress You ll spend far less time trying to optimize your separation Plus the BioLogic LP stores commonly used methods indefinitely so you spend less time preparing your separation ark Biologic LP Because the Biologic LP automatically senses2. BIOLOGIC LP LOW PRESSURE LIQUID CHROMATOGRAPHY SYSTEM A VALUABLE ASSET FOR BIOMOLECULE PURIFICATION Achieving separations is remarkably easy and affordable with our BioLogic LP low pressure chromatography system Here s a workstation that offers numerous user friendly features making it a truly valuable tool for the lab or cold room Incorporating the latest state of the art amenities the Biologic LP System meets the needs of both novice and skilled chromatographers e The user friendly programming and interface make overall operation exceptionally easy e All in one compact design conserves valuable laboratory bench space e From sample injection to fraction collection the BioLogic LP System automates biochromatography it frees you up to concentrate on other tasks e The BioLogic LP System is easy and affordable to maintain The following pages descnbe the BioLogic LP System in detail Leam why it s one of the best assets you can have in your lab COMPONENTS OF THE BIOLOGIC LP SYSTEM Peristaltic Pump UV Monitor Gradient M onitor Proportioning Valve M ixer Inject Valve BioLogic Rack Buffer Select Valve optional Fraction Collector Divert Valve and Column Bypass Valve both optional Dual Pen Chart Recorder optional 60 60006 06 Model 2110 Fraction Collector optional IQUID CHROMATOGRAPHY SYSTEM gi THE BIOLOGIC LP SYSTEM IS PACKED WITH USER FRIENDLY FEATURES
3. of Macro Prep supports Up to three cartridges can be connected in senes to triple sample capacity ALL THE SUPPORT YOU NEED Bio Rad offers a variety of chromatography supports and media for your applications including Macro Prep ion exchange HIC and ceramic hydroxyapatite BioLogic LP Specifications BioLogic LP Ordering Information Pump Flow range 0 1 40 ml min Maximum counterpressure 30 psi 2 bar Gradient formation Solenoid activated proportioning valve Mixer volume 750 ul Detection Performance UV Single channel 280 254 nm with auto zero Detection range 0 001 2 AUFS Conductivity Detection range 0 5 500 mS cm FS Temperature Range 4 40 C Sample loading Manual loop fill Automatic loading through SV 5 Buffer Select Valve Fraction Collector Specifications Model 2110 Fraction Collector Capacity Eighty 13 x 100 mm glass polypropylene or polystyrene test tubes or 1 5 ml micro test tubes when using optional adaptor Collection basis 1 999 drops in 1 drop increments 0 05 9 99 minutes in 0 01 minute increments 10 0 99 9 minutes in 0 1 minute increments Materials of construction Case and carousel polypropylene Front panel hard coated polycarbonate Drop former silicone rubber Waste outlet tube polyurethane Operating temperature 4 40 C Dimensions 24x33x25cm W xD XH Safety certification Meets 1 E C 1010 and C S A 22 2 Model 2128 Fraction Collector Fractionation Time drop or volume Tim
4. ce 110 2 7 VD Bio Rad a ei Laboratories Life Science Group U S 800 4BIORAD California 510 741 1000 Australia 02 9914 2800 Austria 1 877 89 01 Belgium 09 385 55 11 Canada 905 712 2771 China 01 2046622 Denmark 39 17 9947 Finland 90 804 2200 France 1 49 60 68 34 Germany 089 31884 0 Hong Kong 7893300 India 91 11 461 0103 Italy 02 21609 1 Japan 03 5811 6270 The Netherlands 0318 540666 New Zealand 09 443 3099 Singapore 65 272 9877 Spain 91 661 70 85 Sweden 46 0 8 627 50 00 Switzerland 01 809 55 55 United Kingdom 0800 181134 SIG 072296 P 96 0155 REV A 796 Bulletin 2038
5. ct 0 1 10 ml fractions in 13 x 100 mm tubes or 1 5 ml micro test tubes The Model 2110 Collector takes full advantage of the BioLogic LP System s sophisticated windows and threshold collection modes MODEL 1327 CHART RECORDER This dual channel chart recorder features automatically controlled pen lift paper start stop and event mark functions when connected to the BioLogic LP System Its compact flat bed design allows the chart recorder to fit neatly on the BioLogic Rack or alongside the instrument D SUPPORTS TO YOUR SYSTEM GLASS ECONO COLUMN CHROMATOGRAPHY COLUMNS The economical high quality choice for your BioLogic LP System Maximum volumes of columns available range from 1 ml to over 1 liter To optimize your sample application and buffer delivery we recommend adding a flow adaptor to your Econo Column column ECONO PAC CARTRIDGES These prepacked chromatography columns last longer flow faster and often provide better resolution than self prepared columns Their prepacked convenience and low cost make them ideal for e First step purification of crude samples e Methods development an affordable way to test your method before scaling up e Applications requiring frequent column disposal e Single users everyone can have their own set Devoting a cartridge to a single sample eliminating the potential for cross contamination Econo Pac cartndges are available in 1 ml or 5 ml formats packed with a range
6. d volume based programming BUFFER SELECTION MODE Customize the BioLogic LP System to fit your application needs from binary gradient formation to control of up to 5 buffers FRACTION COLLECTOR MODE Customize fraction collection you can collect everything that elutes from a column during the run or specify collection using collection windows a UV threshold value or a combination of both METHODS NAMING MODE Personalize your methods with the alphanumeric naming protocol You can name each method with up to 12 characters and spaces The BioLogic LP System is compatible with additional instruments recommended for top performance This Includes Bio Rad s Valves Fraction Collector Models 2110 and 2128 and the Model 1327 Chart Recorder All are operated by the BioLogic LP Controller SV 5 BUFFER SELECT VALVE For even greater flexibility add the SV 5 Buffer Select Valve to your BioLogic LP System You ll be able to access a total of 5 buffers or samples MoDEL 2128 FRACTION COLLECTOR This state of the art collector accommodates a selection of racks for collection of any sample size expanding your range of applications from analytical to preparative You can collect overlay functions when the Model 2128 is connected to the BioLogic LP System s sophisticated collection control MODEL 2110 FRACTION COLLECTOR Like the BioLogic LP System itself this instrument is highly economical and user friendly enabling you to colle
7. e 0 03 99 9 min in 0 1 min units Drop 0 999 drops Operating modes Time drop time windows 20 peak detection time windows plus peak detection Peak detection Threshold including detection of maxima minima for collection of double peaks above a set threshold Catalog Number 731 8300 731 8301 731 8302 731 8303 731 8304 731 8305 731 8306 731 8307 731 8308 731 8309 Product Description Standard BioLogic LP System 110 120 V includes BioLogic LP Controller BioLogic Rack and Accessory Kit containing inject valve M V 6 proportioning valve mixer Econo UV optics conductivity cell tubing and fittings kit user manual starter kit and conductivity cell column holder Standard BioLogic LP System same as 731 8300 except 220 240 V BioLogic LP System with 2110 Fraction Collector 110 120 V includes BioLogic LP Controller BioLogic Rack and Accessory Kit containing inject valve M V 6 proportioning valve mixer Econo UV optics conductivity cell tubing and fittings kit user manual starter kit and conductivity cell column holder plus M odel 2110 Fraction Collector BioLogic LP System with 2110 Fraction Collector 220 240 V BioLogic LP System with 2128 Fraction Collector 110 120 V includes BioLogic LP Controller BioLogic Rack and Accessory Kit containing Inject valve M V 6 proportioning valve mixer Econo UV optics conductivity cell tubing and fittings kit user manual starte
8. r kit and conductivity cell column holder plus Model 2110 Fraction Collector BioLogic LP System with 2128 Fraction Collector 220 240 V BioLogic LP System with 2110 Fraction Collector and chart recorder 110 120 V includes BioLogic LP Controller BioLogic Rack and Accessory Kit containing Inject valve M V 6 proportioning valve mixer Econo UV optics conductivity cell tubing and fittings kit user manual starter kit and conductivity cell column holder plus Model 2110 Fraction Collector and M odel 1327 Chart Recorder BioLogic LP System with 2110 Fraction Collector and chart recorder 220 240 V BioLogic LP System with 2128 Fraction Collector and chart recorder 110 120 V includes BioLogic LP Controller BioLogic Rack and Accessory Kit containing Inject valve M V 6 proportioning valve mixer Econo UV optics conductivity cell tubing and fittings kit user manual starter kit and conductivity cell column holder plus Model 2110 Fraction Collector and Model 1327 Chart Recorder BioLogic LP System with 2128 Fraction Collector and chart recorder same as 731 8308 except 220 240 V Flow rate capacity Up to 100 ml min counts drops up to 5 ml min Detector input 0 10 mV 0 100 mV 0 1 V Wetted parts All non metal including Teflon Tefzel PPS PEEK Kel F Dimensions 30 5 x 42 x 24 cm W x D x H unit with Rack 1 Safety certification Meets I E C 1010 and C S A 22 2 All specifications subject to change without noti
9. valves there s no need for you to configure the system before writing and running a method the BioLogic LP workstation does it for you In addition delay volume is completely programmable the fraction collector tubes and chart recorder fraction advance marks are synchronized so you know exactly which tubes contain the peak of interest Method ENZYME A Times 75 00 min Duri gt Fraction Collactos LIST OF HEW SHUE ME THe 1 num Wanu METHOD METHOD Char Record ES YOU THROUGH THE PROCESS The BioLogic LP System makes it easy to accomplish multiple tasks Three programmable alarms let you program alert signals informing you when an action is needed during the separation process The alarm and hold functions allow you to program the system to hold a method in progress until a corrective action has been completed You re free to concentrate on other projects knowing you ll be alerted when the separation requires attention LED SCREEN COMMUNICATES EACH MODE An easy to read and follow display is just one of the features that makes the BioLogic LP simple for everyone in the lab to use METHODS MODE Store up to 50 methods on the BioLogic LP System Each method can contain as many as 50 pump steps up to 50 collection windows with or without threshold values and three programmable alarms TIME VOLUME BASED PROGRAM MODE For greater application flexibility choose between time an
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