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Maintaining Your Agilent 1100 Series HPLC System

1. How to Videos Show you how to perform common installation and maintenance procedures for Agilent products Chromatogram Library A searchable collection of GC LC and CE chromatograms for nearly a thousand chemical compounds Instrument and Software Demos Such as product videos 3D animations and interactive software demos www agilent com chem 1100 Series LC System Recommended Maintenance Schedule Pumps ITEM PTFE frits in purge valve Solvent inlet filter Piston seals outlet gold seal plastic cap wash seal gasket wash seal Piston cartridge active inlet valve outlet ball valve Autosampler ITEM Guard column Piston seal isolation seal stator face Column ITEM Needle and needle seat rotor seal Column inlet fittings Detectors ITEM DAD and MWD Detectors Flow cell VWD Detectors IFlow cell TYPICAL SCHEDULE Every month Every 6 months Every 12 months Every 24 months TYPICAL SCHEDULE Every 100 500 injections Every 24 months TYPICAL SCHEDULE Every 12 months Every 5 10 column changes TYPICAL SCHEDULE Exchange standard lamps after 1000 hours Exchange long life lamps after 2000 hours Check cleanliness every 6 months Exchange lamp after 1000 hours Check cleanliness every 6 months ACTIONS COMMENTS Before re installation of the purge valve always check the gold seal and replace if deformed Never use the system without a filter installed
2. Needle Up function Ensure the needle is aligned with the seat Insert the new needle seat assembly Press the seat firmly into position Connect the seat capillary fitting to port 5 of the injection valve Use the Needle Down button to position the needle above the seat making sure the needle and seat are properly aligned Install the front cover and select END under Maintenance Function eae www agilent com chem Agilent Autosampler G1313A G1327A G1329A G1313A G1327A G1329A G1313A G1327A G1329A G1387A G1389A G1367A G1377A G2258A G2260A www agilent com chem Needle Assembly Description Needle assembly standard autosampler Needle assembly for use with PEEK seat Needle assembly 900ul upgrade Needle assembly u LC autosampler Needle assembly well plate autosampler green Needle assembly well plate autosampler new blue Needle assembly u well plate sampler Needle assembly dual loop autosampler Needle assembly prep autosampler Part No G1313 87201 G1313 87203 G1313 87202 G1329 80001 G1367 87200 G1367 87201 G1377 87201 G2258 68710 G2260 87201 AUTOSAMPLER MAINTENANCE Compatible with Needle Seat Standard needle seat 0 17mm
3. Seal Wear In Procedure 1 Fill the bottle with 100 ml of isopropanol and place it in the solvent cabinet Then 28 Recommended after replacing standard seals only 5063 6589 Important Do not perform this procedure when installing normal phase seals 0905 1420 It will cause severe damage to the seals Tools Solvent bottle or beaker Isopropanol e AIV adapter 0100 1847 Restriction capillary 5022 2159 place the new pump head s tubing including bottle head assembly into the bottle Screw the adapter to the Active Inlet Valve Connect the inlet tube from the bottle head directly to the adapter quat pumps and binary pumps with selection valves only Connect the restriction capillary to the purge valve Insert the other end of the capillary into a waste container Open the purge valve and purge the system for 5 minutes with isopropanol at a flow rate of 2 ml min pressure of 350 bar Pump for 15 minutes at this pressure to wear in the seals You can monitor the pressure at your analog output signal using the handheld controller ChemStation or any other controlling device connected to your pump 6 Turn OFF the pump flow and slowly open the purge valve to release pressure from the system 7 Disconnect the restriction capillary and reconnect the outlet capillary to the purge valve Reconnect the appropriate tubing to the Active Inlet Valve 8 Rinse your system with the so
4. Green Green Red Green Red Green Red Red Red Red Red Green Green Green Green Green Fittings Pre swaged Pre swaged Pre swaged With fittings 1 end pre swaged 1 end pre swaged 1 end pre swaged 1 end pre swaged 1 end pre swaged 1 end pre swaged 1 end pre swaged 1 end pre swaged 1 end pre swaged 1 end pre swaged With fittings 1 end pre swaged With fittings Pre swaged Pre swaged With fittings Without fittings Without fittings Without fittings Without fittings 1 end pre swaged Without fittings Without fittings Without fittings Without fittings With fittings With fittings Part No G1312 67305 01090 87308 79835 87638 G1328 87600 G1329 87300 01018 67305 01019 67305 G1313 87304 01090 87610 01090 87611 G1313 87305 01090 87304 01048 87302 01090 87305 G1328 87600 G1316 87303 G1316 87300 G1315 87312 G1315 87311 9062 8522 9021 1820 0021 1821 9021 1822 9021 1823 G1316 87303 0021 1816 9021 1817 0021 1818 9021 1819 G1316 87300 5062 8535 www agilent com chem GENERAL SUPPLIES Capillary and Fitting Kits Description Contents Part No Kit includes Qty 1 PEEK capillary 0 17 mm id 1 5m Oty 4 Stainless steel capillary 105 x 0 17 mm Oty 4 Stainless steel capillary 150 x 0 17 mm Qty 2 Stainless steel capillary 200 x 0 17 mm Oty 2 Stainless steel capillary 280 x 0 17 mm Capillary fitting starter Qty 1 Stainless steel capillary 400 x 0 17 mm kit 0 17 mm ID Tubin
5. Length cm Width Recommended Flow Cell 500 nl l pues Flow Cell 10 0 05 Semimicro dita High Pressure Flow Cell Standard 29 Bi Flow Cell gt 40 0 2 Typical Flow Rate 0 05 0 2 ml min 0 2 0 4 ml min 0 4 0 8 ml min 1 2 ml min 0 05 bml min Internal Column Diameter 0 3 1 mm 2 1 3 0 mm 4 6 mm Flow Cell Maintenance Tips Step 1 Remove the capillaries e Perform a wavelength calibration to S 7 length T sa Step 2 Take out the cell a SIDI fn Clean the leak sensors and check MU Ss A da Vs Pa Step 3 Remove the cell screw and gasket side No 4 WV Q e Perform the intensity test and detector Inspect the positioning of the waste tube O Ea cell test These built in tests will help you o hl Exchange the lamp a verify the performance of your detector eo and determine when maintenance Is Perform a dark current and filter test necessary Clean or replace the flow cell windows as needed www agilent com chem 43 DETECTORS G1315 60012 22 Standard Flow Cell and Replacement Parts Item Description Unit Part No Standard flow cell 10mm 13ul 120 bar G1315 60012 Standard flow cell 10mm 13ul 120bar RFID tag G1315 60022 1 Window screw 79883 22402 2 Spring washers 10 pk 5062 8553 3 Compression washer 79883 28801 4 Window holder 79883 22301 5 Quartz window 1000 0488 6 Seal BACK 12 pk G1315 68711 7 Seal FRONT 12 pk G1315 68710 8 Capillary inlet with heat exchanger G1315 87321 9 SS Tubing 0 17 mm
6. quaternary isocratic Outlet ball valve binary Gold seal Outlet caps 4 pk Binary pump outlet valve SS sieve 10 pk Part No G1311 60012 G1312 60012 9001 3707 5062 2485 5063 6505 PUMP MAINTENANCE You should change the outlet ball valve as part of your regular maintenance routine or when the valve is leaking internally Typically if the pressure ripple is unstable you should run a leak test to determine If the outlet ball valve needs to be replaced www agilent com chem 25 PUMP MAINTENANCE Replacing the Active Inlet Valve cartridge All pumps except the G13561A preparative pump You should service the active inlet valve when the pressure ripple is unstable and a leak test confirms a problem with the valve due to internal leaking The active inlet valve contains a cartridge that you can exchange as follows Description 1 Use a 14 mm wrench to remove the active inlet valve 2 Change the cartridge 3 Reinstall the active inlet valve Pistons and Seals Step up to the strength and consistency of sapphire pistons Agilent pistons are made from a high purity monocrystalline sapphire for maximum durability Although ceramic pistons can be manufactured at a lower cost ceramic is a sintered polycrystalline material which can cause undesired variations during the production process Agilent sapphire pistons Are meticulously cut at just the right angle making them
7. 200 mm long G1315 87302 10 Clamp unit G1315 84901 Zero dead volume fitting 0100 0900 Cell repair kit Includes window screw kit 4 mm hexagonal wrench and seal kit G1315 68712 44 www agilent com chem DETECTORS G1315 60011 Semi Micro Flow Cell and Replacement Parts Item Description Unit Part No Semi micro flow cell 6mm 5yul 120 bar G1315 60011 1 Window screw 79883 22402 2 Spring washers 10 pk 5062 8553 3 Compression washer 79883 28801 4 Window holder 79883 22301 5 Quartz window 1000 0488 6 Seal BACK 12 pk 79883 68702 7 Seal FRONT 12 pk G1315 68710 Capillary inlet with heat exchanger 0 12 mm 290 mm long ora 9 Capillary oulet 0 12 mm 200 mm long G1315 87306 10 Clamp unit G1315 84901 Screw M 2 5 4 mm long for cell body clamp 0515 1056 Cell repair kit semi micro Includes window screw Kit 4 mm hexagonal wrench and seal kits G1315 68713 www agilent com chem 45 DETECTORS G1315 60015 Micro High Pressure Flow Cell and Replacement Parts Item DS o A W N 46 Description Unit Micro high pressure flow cell 6mm 1 pl 400 bar Window assembly Seal ring Quartz window Compression washer Spring washers 10 pk Window screw Capillary inlet with heat exchanger 0 12 mm 290 mm long Capillary oulet 0 12 mm 200 mm long Clamp unit Screw M 2 5 4 mm long for cell body clamp Capillary 0 12 mm 150 mm long Capillary 0 17 mm 380 mm long Cell repair kit Includes 1 quartz w
8. 9183 2072 www agilent com chem 6 ml Vials and Caps for 1100 Series Description Unit Clear crimp vial 20mm cap size 100 pk Crimp caps PTFE silicone septa 20mm 100 pk Clear screw vial 16mm cap size 100 pk Clear screw extreme high recovery vials 30 pk 16mm cap size Screw caps 16mm 100 pk PTFE silicone septa 16mm 100 pk PTFE silicone septa pre slit 16mm 100 pk Microvials Description Unit Crimp top microvials with fixed inserts 100 pk Microvial inserts 500 pk Caps Description Aluminum crimp caps clear PTFE red rubber septa Aluminum crimp caps clear PTFE red rubber septa Aluminum crimp caps blue clear PTFE red rubber septa Aluminum crimp caps green clear PTFE red rubber septa Aluminum crimp caps red clear PTFE red rubber septa Polypropylene screw caps blue PTFE red rubber septa Polypropylene screw caps green PTFE red rubber septa Polypropylene screw caps red PTFE red rubber septa Polypropylene snap caps clear PTFE red rubber septa Polypropylene snap caps blue clear PTFE red rubber septa Polypropylene snap caps green clear PTFE red rubber septa Polypropylene snap caps red clear PTFE red rubber septa Polypropylene screw caps blue PTFE silicone septa Polypropylene screw caps green PTFE silicone septa Polypropylene screw caps red PTFE silicone septa www agilent com chem Part No 9301 1419 9301 1425 9301 1377 0188 2757 9301 1379 9301 1378 0188 2758 Part No 9301 1388 930
9. G1313 60004 G1313 27302 5062 2463 G1313 87102 G1313 87203 G1313 68713 9063 6506 Part No G1329 68715 0101 1050 0100 2088 0100 2089 9065 4410 5022 2175 G1377 60002 G1329 87101 G1329 87103 G1329 80001 G1375 87326 AUTOSAMPLER MAINTENANCE AUTOSAMPLER MAINTENANCE G1367A 1100 Series Well Plate Autosampler Description Well plate sampler accessories kit Standard ship kit includes 100 pk 2ml screw top vials amp caps 10 pk 0 5ml 96 well plates tools 380mm 0 17mm capillary CAN cable Needle assembly well plate autosampler green Needle assembly well plate autosampler new for SN greater than DE21001534 Seat assembly 0 17 mm ID 2 3 ul Seat assembly 0 12 mm ID 1 2 ul Loop capillary 100pI Peristaltic pump Tray for 2 well plates 10 2ml vials G1377A 1100 Series Micro Well Plate Autosampler Description Micro well plate sampler accessories kit Standard ship kit includes 100 pk 2ml screw top vials amp caps 10 pk 0 5ml 96 well plates tools 40yl loop 50cm 50um fused silica PEEK capillary 75um seat capillary CAN cable Seat Capillary 50um 150mm Fused silica PEEK with fittings Seat Capillary 100m 150mm Fused silica PEEK with fittings Seat Capillary 75um 150mm Fused silica PEEK with fittings Loop capillary 8pl Loop capillary 40ul SS capillary 0 25mm ID 12cm Tool for Micro seal capillary mounting Part No G1367 68705 G1367 87200 G136
10. the most durable and longest lasting pistons in the world Are precisely aligned in their stainless steel holder to minimize wear on the piston and seal Cartridge for active inlet valve Active Inlet Valve for 1100 Part No 5062 8562 G1312 60010 Agilent seals are designed to fit snugly around our pistons and are capable of adapting to a wide range of flow rates and pressures Agilent piston seals Are spring loaded and engineered to deliver optimal performance over highly dynamic flow and pressure ranges Are manufactured from a proprietary polymer blend and feature a spring made from the same high quality stainless steel that is used in our pump s flow path The combination of our piston and seal has undergone extensive testing under temperature stress with all common HPLC solvents and in many instruments More importantly they yield consistent reproducible results in highly demanding 26 www agilent com chem PUMP MAINTENANCE Changing the Pump Seals 1 Disassemble the pump head and use a piston to remove the two seals ES E All pumps FEED me 2 Clean the pump chambers and seal o G1361A preparative pump areas by flushing with solvent and Pump seals should be replaced when there wiping with a lint free cloth are leaks on the bottom of the pump head when retention times are inconsistent or when the pressure ripple is unstable oO Insert new seals D Reassemble the
11. 1 U S and Canada or Visit www agilent com chem 1quietcover www agilent com chem Pump Maintenance Achieve maximum uptime by following our recommended procedures The Agilent 1100 Series HPLC system has several different pump units to choose from isocratic binary quaternary capillary and preparative Additionally you can access all pump parts by simply removing the front cover In this section you Il find step by step instructions for the following routine maintenance procedures along with part numbers and ordering information to help you save time Replacing the PTFE frit Cleaning the outlet ball valve e Replacing the AIV cartridge Replacing the seals and pistons Cleaning or replacing the solvent inlet filters You may perform these procedures all at once or as needed Keep in mind though that some parts may need replacing more often than others depending upon your application and solvent preparation procedures Helping our customers meet their challenges requires an understanding of their needs as well as the technical aspects of their work Ultimately know that my answers and suggestions will directly affect our customers success John R Palmer Chromatography Specialist CATALOG For a complete selection of Injection Valves see Agilent s 2005 2006 Essential Chromatography Catalog Or visit our online catalog at www agilent com chem 6ecatalog 23 PUMP MAINTENANCE
12. Purge Valve Exchanging the PTFE Frit 1100 standard pump modules Purge valves feature a gold seal held in 1 Remove capillary and waste tube place with a plastic cap Small leaks can occur here so you will need to replace the gold seal after the valves have been Use a 14 mm wrench to unscrew the valve removed more than once or twice 3 Remove the plastic cap and l the gold seal When replacing the frit make sure that ARA the cutout side faces up which provides 4 Use tweezers to remove the old frit MAD more surface area on the side facing the and install a new frit b solvent flow Sg Replace the gold seal and the plastic cap 6 Install the valve Pump Maintenance Parts Description Part No 1100 Pump Start Up Kit includes 1 outlet cap 2 PTFE frits 4 piston seals 1 outlet gold seal 2 inlet filters 1 cartridge for active inlet valve G1311 68710 PTFE Frits 5PK 01018 22707 Gold seal outlet 5001 3707 Outlet caps 4 PK 5062 2485 Purge valve assembly SS For 1100 and 1050 pumps G1311 60009 24 www agilent com chem Cleaning or Exchanging the Outlet Ball Valve 1 Remove the capillary from the valve 5 Replace the gold seal and cap 2 Use a 14 mm wrench to 6 Use a 14 mm wrench remove the valve to reinstall the valve 3 Remove the plastic cap and gold seal 4 Clean the ball valve in an ultrasonic bath and replace Or replace with a new valve Description Unit Outlet ball valve
13. internal tray or funnel tray When to Perform Once per year or when you notice signs of damage or wear Once per year or when you notice signs of damage or wear When the needle shows signs of damage or blockage When the needle shows signs of damage or blockage or when using the short needle assembly with high test tubes gt 45mm When the valve is leaking or not switching properly When the flow delay sensor no longer works When defective leaky blocked or contaminated www agilent com chem Trays and Collecting Tubes Hole Diameter mm No of Tubes 30 40 29 60 16 126 12 215 Well plate trays FRACTION COLLECTORS Trays collecting tubes and plates for 1100 Series Fraction Collectors Agilent offers a broad range of trays glass collecting tubes and well plates When deciding what s best for your application consider the size and number of the fractions you want to collect Tube Part No Unit 5042 6458 100 pk 5042 6470 100 pk 5042 6459 100 pk 5022 6532 250 pk 5022 6533 100 pk 5022 6531 250 pk 5022 6534 100 pk Description Tray for 4 well plates cooled Tray for 2 well plates 10 funnels cooled Tray for 2 well plates 10 2ml vials www agilent com chem Dimensions Tray Part No 30 x 100 mm G1364 84503 30 x 48 mm 25 x 100 mm G1364 84504 16 x 100 mm G1364 84505 16 x 48 mm 12 x 100 mm G1364 84506 12 x 48 mm Part No G1364 84501 G1364 84502 G1367 60001 FRACTION COLLECT
14. ml min 0 4 0 8 ml min 1 2 ml min 0 05 5 ml min Internal Column Diameter 1 0 mm 2 1 mm 3 0 mm 4 6 mm www agilent com chem Disassembling and cleaning the cell How to remove the cell 1 Remove both capillaries from the flow cell 2 Unscrew the cell screws and remove the flow cell How to disassemble the cell windows 1 Open the cell with a 4 mm hexagonal key 2 Use tweezers to remove the conical springs and gaskets 3 Remove the cell windows with scotch tape Important Do not remove the flow cell windows with tweezers if you plan to reuse them The tweezers will scratch the windows fragile surface 4 Use ethanol to clean the cell body 5 Use a cotton swab and ethanol to clean the cell windows Note if you work with proteins you may want to try a contact lens cleaner Or simply replace the windows 6 Make sure that no deposits are on the window or cell body Installing the Detector Cell Before you return the flow cell to the instrument module 1 Connect the capillaries 2 Switch on the pump and check the cell for leaks Once you are convinced the cell is leak free 3 Install the flow cell into the instrument 4 Screw down the cell DETECTORS Reassembling the cell windows and cell 1 Insert Gasket 2 Note The hole of gasket 2 is larger than the hole of gasket 1 Install the cell window Insert Gasket 1 Insert the SST ring ao A jw N Install the conical springs Not
15. of productivity you need to perform regular maintenance on your variable wavelength diode array and multiple wavelength detectors In the pages to come you ll discover tips and techniques that will help you keep your detectors operating at maximum efficiency For example you ll learn how to Choose the most compatible flow cell for your unique application Disassemble clean and install the flow cell Change the deuterium lamp and more And of course you Il also find the precision engineered Agilent parts you need to get the job done including a range of Agilent flow cells to fit every type of detector T It s all about finding solutions to analytical problems even under severe time constraints It s also about understanding our customer s environment completely so we can put the highest quality products and deepest application knowledge to work within that environment Friedrich Mandel Senior Application Chemist Mass Spectrometry www agilent com chem 35 DETECTORS 36 Variable Wavelength Detector It is critical to verify the function of the VWD on a regular basis There are two built in tests to help you accomplish this The wavelength calibration test The lamp intensity test If the lamp intensity has diminished more than is tolerable for your application you will need to replace the lamp Additionally you should perform the following routine maintenance procedur
16. snap top vial 2ml Clear Crimp snap top vial 2ml Clear Crimp snap top vial 2ml Clear silanized Crimp snap top vial 2ml Clear write on spot Crimp snap top vial 2ml Clear write on spot Crimp snap top vial 2ml Clear write on spot silanized Crimp snap top vial 2ml Amber write on spot Crimp snap top vial 2ml Amber write on spot Crimp snap top vial 2ml Amber write on spot silanized Screw top vial Screw top vial Screw top vial Screw top vial Screw top vial Screw top vial Screw top vial Screw top vial Screw top vial 2ml Clear 2ml Clear 2ml Clear silanized 2ml Clear write on spot 2ml Clear write on spot 2ml Clear write on spot silanized 2ml Amber write on spot 2ml Amber write on spot 2ml Amber write on spot silanized Unit 100 pk 1000 pk 1000 pk 100 pk 1000 pk 100 pk 100 pk 1000 pk 100 pk 100 pk 1000 pk 100 pk 1000 pk 100 pk 100 pk 1000 pk 100 pk 100 pk 1000 pk 100 pk 100 pk 1000 pk 100 pk 100 pk 1000 pk 100 pk 100 pk 1000 pk 100 pk Part No G1375 87320 G1375 87321 G1375 87322 G1375 87323 G1375 87324 G1375 87325 G1375 87327 G2228 68700 Part No 0181 3375 0183 4491 0183 4494 0182 0543 0183 4492 0183 4495 0181 3376 0183 4493 0183 4496 0182 0567 9301 0978 0182 0544 0183 4504 0183 4507 0182 0546 0183 4505 0183 4508 0182 0545 0183 4506 0183 4509 0182 0714 0183 2067 0183 2070 0182 0715 0183 2068 0183 2071 0182 0716 0183 2069
17. whatever it takes to find the most effective answers Hiroki Kumagai www agilent com chem 49 GENERAL SUPPLIES Ready to Use Econofilter Membrane Syringe Filters Agilent s industry standard 25 mm diameter Econofilters are an ideal choice for high sample throughput laboratories We recommend using them to clarify small volume HPLC samples that need further analysis or when particulate matter in the sample solution may cause problems Features include The same quality membranes found in Agilent s high performance syringe filters An inert polymeric housing that helps to spread the sample over the membrane s entire surface so that the maximum capacity is reached No glue or binders used during construction which ensures that no extractables are present Built in simplicity just attach the Luer tipped syringe filled with the sample solution to the housing and push the sample through the pre cleaned filters Econofilters have a moderately wide cross sectional area 4 2 cm with a holdup volume of less than 50 microliters Popular pore sizes of 0 20 and 0 45 micron are available in packages of 200 units Regenerated cellulose nylon and PTFE polytetrafluoroethylene membranes are also available in these configurations Membrane Econofilters 200 pk Description Part No Econofilter Regenerated Cellulose 25 20 5185 5830 Econofilter Regenerated Cellulose 25 45 5185 583
18. 0 SS capillary 0 6x111 mm 1 16 male SS capillary 0 6x40 mm 1 16 male SS capillary 0 6x367 mm 1 16 male G1311 12 54A Pump Accessories Description Bottle Head Assembly Filter for F29 32 tapered inlet bottle Solvent reservoir 1L F29 32 Bottle Head Assembly Screw bottle with drawing tube and filter Solvent reservoir 1L Solvent Reservoir 1L with cap Solvent reservoir amber 1L for use with bottle head assembly Solvent mixer 1100 Series Capillary damper to purge valve G1361 67304 G1361 67305 G1361 67306 Part No G1312 68706 9301 0656 G1311 60003 9301 1420 9301 1421 9301 1450 G1312 87330 G1312 67301 www agilent com chem PUMP MAINTENANCE Solvent Filtration Unfiltered solvents or solvents contaminated with microbial To avoid these problems we recommend these precautions growth especially aqueous buffer solutions present a common problem in HPLC Aqueous media encourage microbial growth Use sterile solvent bottles if possible that can clog the solvent inlet filter and the HPLC particulates in unfiltered solvents can have the same affect Both microbial growth and particulates can also impact the performance of the Replace solvents every two days pump For instance blocked solvent inlet filters can cause erroneous mobile phase composition which will impact peak elution time In addition if the pump keeps working while the filters are blocked the pump could dra
19. 1 Econofilter Nylon 25 20 5185 5832 Econofilter Nylon 25 45 5185 5833 Econofilter PTFE Membrane 25 20 5185 5834 Econofilter PTFE Membrane 25 45 5185 5835 50 www agilent com chem GENERAL SUPPLIES LC Tubing and Fittings What is the Function of LC tubing How can minimize problems with my LC tubing LC tubing creates the sample flow path through your LC system Choose the narrowest diameter and shortest length that your from the autosampler or manual injection valve through application and system will allow This will help prevent peak the column to the detector The ideal tubing provides an dispersion or resolution loss inert surface and leak free connections while eliminating Select smaller id tubing 0 12 mm for narrowbore dead volumes or microbore applications j Mundo advantages foes AYNEN CURING orkar e Use color coded tubing or keep track of identifying Agilent stainless steel and PEEK tubing each characteristics to facilitate maintenance and troubleshooting rovide their own unique benefits sue p 4 How do choose the correct fittings Universal Length Stainless Steel Tubing is precisely measured and pre cut according to Agilent s exact specifications Additionally Agilent 1100 capillary tubing is color coded based on internal diameters e 1 16 OD PEEK Tubing is flexible and easy to cut to desired lengths It s also color coded for easy tracking Additionally PEEK
20. 1 1387 Unit 100 pk 1000 pk 100 pk 100 pk 100 pk 100 pk 100 pk 100 pk 100 pk 100 pk 100 pk 100 pk 100 pk 100 pk 100 pk GENERAL SUPPLIES Part No 9181 1210 0183 4498 0181 1215 0181 1216 0181 1217 0182 0717 0182 0718 0182 0719 0182 0550 0182 3458 0182 3457 0182 3459 5182 0720 0182 0721 9182 0722 59 GENERAL SUPPLIES 60 Vial Kits Description Part No 2 ml vial kit includes 100 vials 01078 68705 100 crimp caps 1 11 mm crimper Clear crimp top vials silver aluminum crimp caps PTFE red rubber septa in 6 drawer storage box 500 pk J i Amber crimp top vials with write on spot silver aluminum crimp caps PTFE red rubber 5181 8801 septa in 6 drawer storage box 500 pk Electronic Crimper For 2 mL crimp top autosampler vials Agilent s electronic crimper allows you to crimp vials without removing them from their common autosampler trays It also lets you crimp hundreds of vials with a single battery charge Features include An electric motor for reproducible crimps Hand held push button operation Electronic crimp force adjustment e A rechargeable VersaPak Gold Nickel Metal Hydride battery from Black amp Decker Ergonomic design to minimize wrist fatigue Description Part No Electronic Crimper for 2ml 12x32mm crimp vials and 11mm crimp caps with Black amp Decker VersaPak gold battery and charger 5183 4763 Electronic Decapper for 2ml 12x32mm crimp vial
21. 65054 79853 27200 79853 68734 www agilent com chem DETECTORS Diode Array Detector DAD Multiple Wavelength Detector MWD DAD Routine Maintenance Procedures Perform the intensity test and detector cell test These built in tests will help you verify the performance of your diode array and determine when maintenance is necessary Clean or replace the flow cells as needed Perform a wavelength calibration to ensure wavelength accuracy Clean the leak sensors and check their function Inspect the positioning of the waste tube Exchange the lamp e Perform a dark current and filter test www agilent com chem 41 DETECTORS Changing the Deuterium Lamp You should replace the deuterium lamp when the baseline noise and or drift Description Part No exceed your application limits or if the lamp does not light Use the following Deuterium lamp 1100 DAD MWD longlife 5181 1530 procedure 1 If the detector has been in use turn it off Deuterium lamp 1100 DAD MWD 2140 0590 and allow it to cool before proceeding 2 Remove the front cover Tungsten Lamp assembly G1315A 8453 G1103 60001 3 Disconnect the electrical connection and unscrew the lamp to remove it Deuterium lamp 1100DAD MWD long life A 2140 0813 from the DAD 4 Replace with a new lamp taking care Deuterium lamp 1100 DAD MWD long life not to touch the glass surface with CFor G1315C DAD and G1365C MWD yo
22. 7 0 17 0 17 0 17 0 17 0 17 0 17 0 25 0 25 0 50 0 50 0 50 0 50 www agilent com chem Length cm 10 5 15 Color Code Red Red Red Red Red Red Green Green Green Green Green Green Green Green Blue Blue None None None None Volume ul Universal 1 7 2 3 3 2 4 5 5 6 2 4 3 4 4 6 6 4 9 1 13 6 15 9 20 5 13 8 39 3 20 6 29 5 55 157 Part No 5021 1820 5021 1821 5065 9935 5021 1822 5021 1823 5065 9964 5021 1816 5021 1817 5065 9931 5021 1818 5021 1819 5065 9933 5065 9932 5065 9963 5022 6508 5065 9930 5065 9927 5022 6509 5022 6510 5065 9926 GENERAL SUPPLIES 53 GENERAL SUPPLIES Flexible Capillary Connectors From Pump Autosampler Manual valve Column compartment Column VWD 54 To Autosampler Manual Valve Universal Column Column Column DAD VWD Universal Outlet ID mm 0 17 0 25 0 25 0 17 0 17 0 25 0 25 0 12 0 12 0 12 0 17 0 17 0 17 0 17 0 17 0 12 0 17 0 12 0 17 0 18 0 12 0 12 0 12 0 12 0 12 0 17 0 17 0 17 0 17 0 17 0 25 Length cm 60 13 32 50 90 70 70 18 28 10 5 18 28 80 13 50 15 38 40 10 5 15 28 40 10 5 15 28 40 48 Material SS SS SS SS SS SS Ta SS SS SS SS SS SS SS SS SS SS SS SS PEEK SS SS SS SS SS SS SS SS SS SS PEEK Color Code Green Blue Blue Green Green Blue Blue Red Red Red Green Green Green
23. 7 87201 G1367 87101 G1367 87102 G1367 87300 5065 4445 G1367 60001 Part No G1377 68705 G1375 87300 G1375 87317 G1375 87316 G1375 87315 G1377 87300 G1377 87301 G1377 44900 www agilent com chem www agilent com chem Fraction Collectors Reliable rugged and easy to use Agilent 1100 Series fraction collectors are designed to meet stringent standards for collection capability So you never have to compromise on recovery and purity In this section you ll find essential facts about our analytical scale AS and preparative scale PS fraction collectors including Fraction collector maintenance plus simple repairs you can perform without a service call Agilent engineered parts such as trays collecting tubes plates and needle assemblies Guidelines for maintaining the micro fraction collector spotter e MALDI spotting accessories Part numbers ordering information and much more You can also visit www agilent com chem for more details about fraction collection performance software solutions and application guidelines We manufacture our HPLC systems from the ground up and provide our customers with a wide variety of columns consumables and supplies built to Agilent s precise specifications We re also a great resource for all types of applications in fact we re developing new ones every day You might say that we re your partner in chromatography Ron M
24. A G1329A G1389A G1367A G1377A G2260A G1367A G1377A Includes 1 rotor seal Vespel 1 needle 1 needle seat 2 metering seals 15 fingercaps Accessory kit Includes 3 hex keys 2 wrenches tubing 1 label halftray wrist strap 15 fingercaps 0 17id x 180mm capillary 100 screw top 2ml vials and caps tray for 40 2ml vials tray for 15 6ml vials Preventative maintenance kit Includes 1 each rotor seal isolation seal stator face needle needle seat Part No G1313 44500 G1313 44502 G1313 44503 G1329 60001 G1367 60001 Part No G1313 68709 G1313 68705 0065 4498 www agilent com chem G1313 27 29A 1100 Series Autosampler Supplies Description External Vial Tray for 17 vials disposal position Disposal Tube for external vial tray Waste tubing 5 m 6 5 mm ID corrugated polypropylene Needle seat PEEK 0 17 mm ID capillary for standard autosampler 2 34 Needle assembly for use with PEEK seat Needle arm kit Finger Caps 15 pk G1387A 1100 Series Micro Autosampler Description Capillary sampler accessories kit Injection valve for 1100 LC System Rotor seal Vespel 2 grooves for LC Stator for p LC valves special hardened 6 fittings 2 plugs PEEK for p valves Piston seal 2mm Seal support assembly Seat assembly 100um ID 1 2u l Seat assembly 50um ID 0 3 pl Needle assembly for p LC autosampler Waste tube FEP 1 6mm OD 0 8mm ID www agilent com chem Part No
25. Conical spring Ring SS Gasket 1 PTFE Quartz windows Gasket 2 PTFE Unit 10 pk 2 pk 10 pk 2 pk 10 pk Part No G1314 65050 79853 27200 79853 29100 79853 22500 79853 68743 79853 68742 G1314 65051 G1314 60083 Semi micro Flow Cell Replacement Parts Item www agilent com chem Description Semi micro flow cell assembly 6 mm DuL 40 bar Semi micro flow cell kit Includes 2 windows 4 gaskets 2 standard 1 1 semi micro 1 1 semi micro 2 Cell screw Conical spring Ring SS Quartz windows Unit 10 pk 2 pk 2 pk Part No G1314 60083 G1314 65056 79853 27200 79853 29100 79853 22500 79853 68742 DETECTORS 39 DETECTORS G1314 60081 Micro Flow Cell Replacement Parts Item Description Unit Micro flow cell 5 mm 1yL 40 bar Micro flow cell kit Includes 2 windows 2 gaskets 1 2 gaskets 2 Cell screw Conical spring 10 pk Ring SS 2 pk Quartz windows 2 pk Gasket 1 PTFE 10 pk Gasket 2 PTFE 10 pk Part No G1314 60081 G1314 65052 79853 27200 79853 29100 79853 22500 79853 68742 79853 68743 G1314 65053 G1314 60082 High Pressure Flow Cell Replacement Parts Item 40 Description Unit High pressure flow cell 10 mm 14 uL 400 bar High pressure flow cell kit Includes 2 windows 2 Kapton gaskets 2 PEEK rings Cell screw Window UHP 2 pk Part No G1314 60082 G1314
26. Extended instrument life P e Reduced administrative burdens e Fast problem resolution If you choose you can also consolidate instrument maintenance and repair services into multi year agreements for administrative ease and greater cost savings 62 www agilent com chem Agilent Services Telephone Service Software Service Onsite Instrument Repair For selected Agilent instruments Offsite Instrument Repair For selected Agilent instruments Preventative Maintenance Installation Qualification 1Q www agilent com chem Features Telephone access to skilled Agilent service professionals Available parts option Telephone access to trained technical professionals Enhancements to the original application software purchase Software bulletins that identify discovered defects and recommend workarounds Hardware telephone support with access to skilled factory trained Agilent service professionals Travel expenses and labor Optional coverage for consumables used during repair Option 1 Replacement with an identical instrument your fastest choice Option 2 Return to Agilent for repair Expert cleaning adjustment lubrication and inspection of your instrument Travel expenses and labor Coverage for consumables used Qualification and documentation of shipment completeness Comprehensive system and application software verification tests Delivered by professionals with training certification SERVICE
27. ID capillary 2 3pl Standard needle seat 0 12 mm ID capillary 1 2 pl Standard needle seat PEEK 0 17 mm ID capillary 2 3 pl Standard needle seat 0 17mm ID capillary 2 3pl u LC Needle seat 100 um ID capillary 1 2 pl u LC Needle seat 50 um ID capillary 0 3 pl Needle seat well plate autosampler 0 17 mm ID capillary 2 3 pl Needle seat well plate autosampler 0 12 mm ID capillary 1 2 pl Needle seat u well plate autosampler without seat capillary Seat Capillary 100um 150mm Fused silica PEEK with fittings Seat Capillary 75um 150mm Fused silica PEEK with fittings Seat Capillary 50um 150mm Twin needle seat dual loop autosampler Needle seat prep autosampler 0 5 mm ID 20 ul Part No G1313 87101 G1313 87103 G1313 87102 G1313 87101 G1329 87101 G1329 87103 G1367 87101 G1367 87102 G1377 87101 G1375 87317 G1375 87316 G1375 87300 G2258 87102 G2260 87101 AUTOSAMPLER MAINTENANCE Autosampler Parts and Supplies Agilent offers a variety of supplies that increase the flexibility of your 1100 System Trays for 1100 Series Autosamplers Description Tray for 100 2ml vials Tray for 40 2ml vials Tray for 15 6ml vials Tray for 100 2ml vials thermostattable Tray for 2 well plates 10 2ml vials G1313 27A 1100 Series Autosampler Kits Description Maintenance kit Used In instrument G1313A G1367A G1377A G1313A G1313A G1329A G1389A G2260
28. ORS G2255 68700 5022 6538 5022 6539 Well plates Description 96 Well plates 0 5ml Polypropylene 96 Well plates 0 5ml Polypropylene 96 Deep well plates 1 0ml Polypropylene Closing mats for 96 well plates silicone 96 Well Plates 150ul conical 384 well plates Polypropylene 96 Deep well plate with glass inserts caps septa Glass inserts 350ul Caps septa for glass inserts Vial plate for 54 x 2 ml vials Tray for 27 Eppendorf safe lock tubes 0 5 1 5 2 ml Vial plate for 15 x 6 ml vials Unit 120 pk 10 pk 50 pk 50 pk 25 pk 30 pk 1000 pk 1000 pk 6 pk Part No 5042 1385 5042 1386 5042 6454 5042 1389 5042 8502 5042 1388 5065 4402 5188 5321 5188 5322 G2255 68700 9022 6538 5022 6539 www agilent com chem FRACTION COLLECTORS Needle Assembly Description Item Description Part No 1 Needle assembly preparative scale G1364 87201 2 Needle assembly analytical scale 20 mm G1364 87202 3 Needle assembly analytical scale 50 mm G1367 87200 4 Capillary guiding assembly micro scale G1364 87303 www agilent com chem 19 FRACTION COLLECTORS Maintaining The Micro Fraction Collector Spotter Agilent offers three types of connecting capillaries each with different internal diameters We recommend replacing the capillaries and flap septum every six months or when contaminated blocked worn
29. Operate your lab at peak performance Troubleshoot difficult problems Select the right Agilent supplies Maintaining Your Agilent 1100 Series HPLC System Maximize efficiency Minimize your downtime Get the results you need We re committed to bringing you the world s best HPLC systems Plus the critical information you need to keep them running properly As an Agilent customer you have complete access to over 40 years of industry knowledge and experience With a wide range of technical know how and a never ending supply of ideas our goal is to help you solve your most challenging application problems That s why we created Maintaining Your Agilent 1100 Series HPLC System It s designed to help you keep your HPLC system running at optimal performance and to ensure that your manual injection valves autosampler pump and detectors always work at peak efficiency Inside you ll find Essential facts about Agilent s 1100 Series HPLC consumables and accessories Number in HPLC e Vital maintenance procedures and troubleshooting tips iy Agilent Technologies All the Agilent part numbers you ll need in one easy to use guide So you ll expand your system s hours of continuous operation decrease downtime and get the reproducible results that your lab demands Maintaining Your Agilent 1100 Series HPLC System Ensuring optimal performance and results 1 Section 1 Manual 35 Section 5 Detector Injection Valv
30. PN 5067 1503 reproducible start signal 1 0 mm diameter passages allow flow rates up to 800 ml min with virtually no pressure drop Make Before Break technology allows switching without flow interruption Manual Injection Valves with Position Sensing Switches Description Part No 77251 SS 5063 6502 97251 PEEK 0101 1253 37251 SS prep valve 0101 1232 37251 PEEK prep valve 0101 1231 Manual prep injection valve kit SS 37251 038 with position sensing 10ml loop 25ml syringe ring mounting bracket start cable SS connecting capillaries 0 5mm id 9009492 40cm and 60cm RheBuild Kits RheBuild Kits Used with Rheodyne Series RheBuild kits include all individual parts rotor seal stator face assembly isolation Description Part No seal tools and instructions to completely 7755 inictonvale 0101 1254 refurbish specific valves to ensure high f d long lifetime jecti performance and long lifetime 1750 020 Injection valve 0101 1257 2 groove rotor seal 7750 030 Switching valve 0101 1258 3 groove rotor seal www agilent com chem 3 MANUAL INJECTION VALVES Maintenance and Replacement Parts for Manual Injection Valves Description Rotor Seals Vespel operating pH 0 to 10 7125 7725 Tefzel operating pH 0 to 14 7125 7725 PEEK operating pH 0 to 14 7125 7725 9725 PEEK operating pH 0 to 14 3725 Stator Face Assembly Rheodyne Series 7125 Rheodyne Series 7725 Stators Rheodyne Se
31. RAL SUPPLIES PEEK Coated Fused Silica Capillaries for use with 100 pl min Flow range Item From To Fitting ID um Part No a EMPV Flowsensor B B 100 um G1375 87305 b Flowsensor Injection valve B C 100 um G1375 87306 c Injection valve Metering device B C 100 um G1375 87312 d Metering device Needle B B 100 um G1375 87303 e Injection valve Column C D 75 um G1375 87311 f Injection valve Waste C 100 um G1375 87307 g Column Detector D E 75 um G1375 87308 h Detector Waste E 75 um G1315 68708 switching valve Column C D 50 um G1375 87309 SSV Pumphead A Pumphead B sensor etering evice 7 7 Needle Ras njection valve f ES Column 56 www agilent com chem GENERAL SUPPLIES Replacement Fittings and Ferrules for Capillary and Nano Flow System Graphic Description Type Part No iiinn 1 16 SS fittings front and pus back ferrules 10 pk eee aul Fitting male 1 16 4mm SS 10 pk B 5063 6593 1 32 ferrule and SS lock ring 10 pk B 5065 4423 ii 6 fittings 2 plugs PEEK for valves C 5065 4410 Double winged PEEK nuts and 1 32 ferrules 10 pk a da Generic Connecting Capillaries for Capillary LC System Item From To Fitting Part No 1 SSV AIV G1311 67304 Z Piston 1 Piston 2 A A G1312 67300 3 Pump Restriction capillary A A G1312 67302 4 Mixing capillary Damper A A G1312 67304 D Damper Mixer A A 01090 87308 6 Mixer Filter A A 01090 87308 7 Filter EMPV A A G1375 87400 8 Needle seat In
32. S AND SUPPORT Advantages to You Fast identification and resolution of hardware problems Low cost Fast identification and resolution of software problems A fixed annual cost for software upgrades makes budget management more predictable A single cost effective source for telephone support and software updates A convenient way to keep up with the latest software enhancements A fixed annual cost for parts makes budget management more predictable You can choose the response method that fits your business needs and budget Less expensive than onsite repair Fast and easy Includes extensive diagnostics and testing that are not feasible with onsite maintenance or repair Maximum uptime Extended instrument life Confidence in the integrity of your measurements Supplies the evidence you need to satisfy regulatory agencies Helps fulfill the master validation plan and change control SOP requirements for the IO phase 63 SERVICES AND SUPPORT Agilent Services Operational Qualification Performance Verification 00 PV Requalification RQ After Repair 64 Features Verification and documentation of an instrument s ability to meet specified criteria Procedures and documentation that fit the requirements of GLP ISO 9000 and other regulatory agencies Full automation to increase the qualification scope without excess time requirements Measuring equipment that is traceable to national and inte
33. When piston seals are exchanged check the surface of the piston for scratches and deposits Never re install a used piston seal Pistons can be cleaned with alcohol or toothpaste After exchanging the active inlet valve cartridge pump several mL until the system Is stable ACTIONS COMMENTS Change the guard after 25 50 injections for dirty samples 100 250 injections for most samples and 500 injections for very clean samples ACTIONS COMMENTS Make sure you use rotor seals of the correct material depending on the operating pH PEEK fittings make changing inlet fittings fast and easy SS fittings are reliable and can be used at higher pressure ACTIONS COMMENTS A noisy baseline may indicate it is time to change the lamp Low light intensity can be caused by a dirty flow cell Low light intensity can be caused by a dirty flow cell Low light intensity can be caused by a dirty flow cell www agilent com chem For detailed information about supplies from Agilent please contact your local Agilent sales representative or your authorized distributor or visit us online Ask about Agilent s other valuable resources Agilent ZORBAX Column Selection Guide for HPLC Maintaining your Agilent GC and GC MS Systems Essential Chromatography Catalog Online Library and Tech Support The information and pricing in this guide are subject to change without notice Agilent Technologies Inc 2005 Printed in USA Aug
34. ains how to operate Agilent 1100 Series Liquid Chromatographs using HPLC ChemStation software Provides hands on laboratory exercises on the operation and maintenance of the Agilent 1100 Series Liquid Chromatograph equipped with a Diode Array controlled by the HPLC 3D ChemStation Introduces maintenance and troubleshooting for the Agilent 1100 series modules Introduces troubleshooting and maintenance of the isocratic binary and quaternary pumping systems Also covers the vacuum degasser autoinjector variable wavelength detector and diode array detector Teaches use of Agilent HPLC 2D ChemStation software for data analysis and reporting Teaches use of Agilent HPLC 3D ChemStation software for data analysis and reporting 67 SERVICES AND SUPPORT 68 Support Tap Agilent s LC and LC MS expertise anytime Just give us a Call to discuss hardware software application concerns or basic operation techniques Visit Agilent online For a wealth of knowledge tips and insights go to www agilent com chem You ll find Frequently Asked Questions about Agilent instruments and supplies Interactive Troubleshooter A step by step approach to help you solve common problems Find a Part Includes parts information pricing and availability plus an Illustrated parts breakdown Software Downloads and Utilities Feature patches and status bulletins tools and utilities firmware and revision tables
35. ajors Product Marketing Specialist CATALOG fe For a complete selection of Injection Valves see Agilent s 2005 2006 Essential Chromatography Catalog Or visit our online catalog at www agilent com chem 6ecatalog FRACTION COLLECTORS Analytical and Preparative Fraction Collector Maintenance Fraction collectors spotters are very user friendly and do not require much maintenance However you should perform the following routine procedures Exchange the tubing at least once per year Replace the capillaries Replace the flap septum Tubing Replacement There are two types of tubing available to connect the valve with the needle preparative scale PTFE tubing 0 8 mm id and analytical scale tubing 0 25 mm id Both types should be replaced when they become contaminated worn or damaged Before replacing the tubing be sure to 1 Position the fraction collector s transport unit in the Home position 2 Remove all installed trays from the tray base 3 Position the transport unit in the Change Parts position 4 Turn off the instrument Consult your user s manual for detailed replacement instructions Simple Repairs Procedure Replace the inlet waste tubing Replace the valve to needle tubing Exchange the preparative needle assembly Exchange the analytical needle assembly Exchange the diverter valve Exchange the internal tray Repair or exchange a funnel within the
36. aken into account Important Sample loops for the 7725 Series and 7125 Series are not interchangeable That s because the port angle is 30 for the 7725 and 20 for the 7125 www agilent com chem Description 5 ul 10 pl 20 ul 50 ul 100 gl 200 ul 500 pl 1 ml 2 ml 5 ml Stainless Steel Loops for 7725 Injectors Description 5 ul 10 pl 20 ul 50 ul 200 ul 500 ul 1 ml 2 ml 5 ml Stainless Steel Loops for 7125 and 7010 Injectors ID mm 0 18 0 30 0 51 0 51 0 51 0 76 0 76 0 76 1 00 1 00 ID mm 0 18 0 30 0 30 0 51 0 76 0 76 0 76 1 00 1 00 MANUAL INJECTION VALVES Part No 1535 4860 0101 0376 0101 0377 0101 0378 0101 0379 0101 1252 0101 1251 0101 1219 0101 1250 0101 1249 Part No 0101 1248 0100 1923 0100 1922 0100 1924 0101 1247 0101 1246 0101 1245 0101 1244 0101 1243 MANUAL INJECTION VALVES PEEK Sample Loops PEEK is inert to almost all organic solvents and is biocompatible Like metal loops the actual volumes of PEEK loops can differ from their nominal designations because of the 0 05 mm 0 002 tolerance of the tubing bore Generally accuracy rates are about 14 for large PEEK loops 0 8 mm 0 030 21 for intermediate loops 0 5 mm 0 020 and 65 for small loops 0 2 mm 0 007 Although PEEK is compatible with virtually all solvents many factors can affect the burst pressure of PEEK tubing For example wall thicknes
37. an be sonicated in isopropanol however glass filters cannot be sonicated as glass particles may break off or the filters may shatter To test if a solvent inlet filter is blocked disconnect the solvent inlet tube at the end furthest from the filter and bottle head assembly If the filter is in good condition solvent will flow freely out of the solvent tube If there is a blockage little or no solvent will drip from the tube Inlet Frit Part No Description Glass filter solvent inlet 5041 2168 20 um pore size 3150 0944 Glass filter solvent inlet 40 um pore size 3150 0944 Glass filter solvent inlet 40 um pore size PUMP MAINTENANCE If a blockage occurs it s easiest to simply replace the solvent inlet filter However you can clean a glass filter by using the following procedure 1 Remove the solvent inlet filters 2 Replace the glass frit filters or soak them in concentrated nitric acid 35 for one hour Be sure to flush well to avoid column damage 3 Install either the new or cleaned solvent filters SUD Frit Inlet TubeOD Recommended Part No Description ID mm mm Use Frit adapter PTFE 3 mm analytical scale 5062 8517 D 3 2 4 pk micro scale G1361 23204 Frit adapter 4mm PTFE 7 4 Preparative LC G2258A Greece ante re to 7 32 Dual Loop 1 8 od tubing autosampler www agilent com chem PUMP MAINTENANCE In Line Filters Column inlet frit contamination can increase co
38. bers and important maintenance information With more than 50 000 systems the Agilent 1100 Series has become the industry s best selling LC system Agilent has earned its reputation for superior technology by designing and developing analytical instruments which produce consistent first class LC results Our wide range of columns consumables and supplies are designed to the same level of quality to ensure day in day out hassle free operation keeping your productivity high and your operating cost low Erich Wagner Product Manager CATALOG For a complete selection of Injection Valves see Agilent s 2005 2006 Essential Chromatography Catalog Or visit our online catalog at www agilent com chem 6ecatalog MANUAL INJECTION VALVES Agilent injection valves from Rheodyne are backed by 40 years of innovation and experience They feature Patented Make Before Break MBB technology that provides uninterrupted flow when switching between LOAD and INJECT positions Additionally MBB greatly reduces transient pressure An injection port built right into the handle This unique design allows the tip of the needle to connect directly to the sample loop So you don t have to worry about sample loss during loading shocks which benefits flow sensitive detectors and extends column life no The reproducibility of manual sample injectors depends on operator skill syringe precision and loading method The partial fill
39. dle seat Tools 1 4 Wrench 2 5 mm Hexagonal key When installing the new needle take care not to strip the setscrew You may have to bend the needle slightly to ensure needle seat alignment www agilent com chem 2 Remove the sample loop Select Needle Down button until the needle screw shows up in the hole of the safety cover Loosen and remove the needle s set screw AUTOSAMPLER MAINTENANCE Stator Part No 0100 1850 Stator head 0100 1851 Stator face 0100 2089 0101 1390 AUTOSAMPLER MAINTENANCE 3 Move the needle arm to NS E the lowest point using e the Needle Down function Install the new needle ES rab Align the needle above S N the needle seat eY Reconnect the sample loop f Exchanging the Needle Seat See manual for well plate Autosampler Before beginning this procedure e Select Change Needle under Maintenance Function e Remove the front cover when the needle is positioned approximately 15 mm over the needle seat 1 Disconnect the seat capillary from the injection valve port 5 2 Use a small flat screwdriver to ease out the needle seat 4 P Situate the needle approximately 2 mm above the seat using the
40. e Install the conical springs with the conical side toward the cell window This will ensure correct placement of the conical springs which is critical to proper tightness 6 Carefully tighten the cell screw The cell repair kit includes the required cell windows pack of 2 and gaskets We recommend the following tests after cell installation Zero order calibration Wavelength calibration Because the flow cell is part of the optical system and has now been moved since these tests were last performed we recommend running them again before your next analysis There are two designs of the Standard Flow Cell The cell screw of the new design G1314 60086 is painted black www agilent com chem 37 DETECTORS G1314 60086 Standard Flow Cell Replacement Parts 1 Item Description Unit Part No O A Standard D type flow cell 10 mm 14uL 40 bar 61314 60086 a Standard D flow cell kit Includes 2 windows 2 G1314 65061 OA gaskets 1 2 gaskets 2 1 1 Cell screws 2 pk G1314 65062 2 Conical spring 10 pk 79853 29100 3 Ring 1 PEEK 2 pk G1314 65065 4 Gaskets 1 Kapton 10 pk G1314 65063 5 Quartz windows 2 pk 79853 68742 6 Gaskets 2 Kapton 10 pk G1314 65064 7 Ring 2 PEEK 2 pk G1314 65066 38 www agilent com chem G1314 60080 Standard Flow Cell Replacement Parts Item Description Standard D flow cell kit Includes 2 windows 2 gaskets 1 2 gaskets 2 Cell screw
41. ernet access You can review and register for our e Learning offerings by visiting www agilent com chem elearning 66 www agilent com chem Classroom Training Agilent s training courses for liquid chromatography and mass spectrometry help new and experienced lab professionals learn proper and efficient ways to use analytical instruments and software These ISO registered courses also SERVICES AND SUPPORT Contact Agilent today for more information about our training and services Or visit www agilent com chem and select Education target those who want to broaden or sharpen their troubleshooting maintenance and system operation skills Course Title Course No Practical High Performance Liquid Chromatography H5930A Techniques of High Performance Liquid Chromatography H1186A Introduction to Liquid Chromatography and the HPLC ChemStation H5929A Agilent 1100 Series HPLC 3D ChemStation Operation H4033A Agilent 1100 Series HPLC Troubleshooting and Maintenance H8969A Agilent 1100 Series HPLC Routine Maintenance H4058A Agilent 1100 Series HPLC 2D Data Analysis and Reporting H5928A Agilent 1100 Series HPLC 3D Data Analysis and Reporting H4039A www agilent com chem No of Days Description Introduces the basics of liquid chromatography in a lecture format Presents the fundamental concepts of liquid chromatography in a laboratory and lecture format Expl
42. es Check the waste outlet Clean the leak sensor Check the photocurrent Clean or replace the flow cell windows when they become dirty Choosing the Optimal Flow Cell For Your Variable Wavelength Detector Agilent offers four flow cell options for the VWD Use the chart below to select the appropriate one for your application keeping the following guidelines in mind VWD Flow Cell Selection Typical Column Typical Peak Description G1314A VW detector accessory kit Includes 1 4in waste tubing 2 hex keys 2 wrenches outlet tubing 1 16in PEEK male fitting Deuterium lamp 1000 hours Deuterium longlife lamp VWD inlet tubing with fitting 0 18 x 40mm PEEK VWD outlet tubing with fitting 0 25 x 48mm PEEK If more than one selection is appropriate choose the larger flow cell to get the best detection limit For columns capable of providing narrower peak widths 1 8 um particle size the smaller flow cell may be more appropriate Part No G1314 68705 G1314 60100 2140 0813 5062 8522 5062 8535 Flow cells with longer path lengths provide better signal detection However a longer path length also increases the cell volume which causes more peak dispersion Length cm Width Recommended Flow Cell lt 5 0 025 R a OE High Pressure Semimicro Flow Cell E o Flow Cell Standard For Pressure 20 0 1 Flow Cell Above 100 bar gt 40 0 2 Typical Flow Rate 0 05 0 2 ml min 0 2 0 4
43. es Maintenance 5 Sample Loops 36 Variable Wavelength Detector 41 Diode Array Detector DAD 7 Section 2 Autosampler De IMD O Maintenance 8 Injection Valve Maintenance EMI M DONOS 9 Exchanging the Needle Needle Seat 49 Section 6 General Supplies 12 Autosampler Parts 50 Ready to Use Econofilter and Supplies Membrane Syringe Filters 51 LC Tubing and Fittings 15 Section 3 Fraction Collectors 53 Universal Connecting 16 Analytical and Preparative CRANES Fraction Collector Maintenance 60 Electronic Crimper 19 Needle Assembly Description 20 Maintaining The Micro Fraction 61 Section 7 Services Collector Spotter and Support 66 Education amp Training Services 23 Section 4 Pump Maintenance 68 Support 24 Purge Valve 25 Cleaning or Exchanging the Outlet Ball Valve 26 Replacing the Active Inlet Valve cartridge 26 Pistons and Seals 29 Parts and Supplies 31 Solvent Filtration 33 HPLC In line Filters 34 Vacuum Degasser Parts and Supplies www agilent com chem www agilent com chem Manual Injection Valves Meticulously engineered to ensure trouble free operation You can count on Agilent injection valves just like you depend on your HPLC System That s because our valves are built to Agilent s industry leading standards to ensure consistent results and optimum performance from your analytical instruments In this section you ll find the latest in LC injection valve technology along with part num
44. g cutter for PEEK capillaries 1 16 in stainless steel fittings 10 pk 1 16 in PEEK fittings color 10 pk 1 16 in PEEK fittings 10 pk Qty 3 ZDV union stainless steel Rheotool Cybertool 0065 9939 Kit includes PEEK capillary 0 13 mm id 1 5m Qty 4 Stainless steel capillary 105 x 0 12 mm Qty 4 Stainless steel capillary 150 x 0 12 mm Qty 2 Stainless steel capillary 200 x 0 12 mm Qty 2 Stainless steel capillary 280 x 0 12 mm Capillary fitting starter Stainless steel capillary 400 x 0 127 mm kit 0 12 mm ID Tubing cutter for PEEK capillaries 1 16 in stainless steel fittings 10 pk 1 16 in PEEK fittings color 10 pk 1 16 in PEEK fittings 10 pk Oty 3 ZDV fitting Rheotool Cybertool 5065 9937 Kit includes Qty 2 Fused silica PEEK capillary 50um 55 cm Qty 1 Fused silica PEEK capillary 50um 20 cm Qty 1 Fused silica PEEK capillary 100um 110 cm Capillary fitting starter Qty 2 Fused silica PEEK capillary 50um 50 cm kit for 1100 Capillary LC Qty 2 Fused silica PEEK capillary 50um 40 cm 5065 9938 System Qty 4 4 mm stainless steel fitting male 10 32 Qty 4 1 32 in PEEK ferrule and stainless steel lock ring Oty 4 PEEK fittings for valves Qty 4 Double winged PEEK nuts and 1 32 in ferrules Qty 1 Cybertool Rapid Resolution High Kit includes Throughput capillary kit Qty 5 Capillaries to optimize the 1100 Series LC for use with RRHT columns 9065 9947 www agilent com chem 55 GENE
45. icro grit paper Precision glass capillary for the G1946B and G1946C Precision glass capillary with platinum ends for G1946D Filter assembly 5 um replacement frits for P N G1946 60180 box of 5 filters High pressure semi prep filter 10 um stainless steel frits for P N 5022 2165 10 pk Rhebuild kit for active splitter kit Includes 3 rotor seals and 3 stator face assemblies for G1968 64102 Part No 05971 80103 G2440 60200 6040 0834 G2427A G2428A G1946 20213 G1947 20029 05980 60051 8660 0852 G1946 80009 59987 20040 G1946 60180 0100 2051 5022 2165 5022 2166 1535 5215 05971 80103 www agilent com chem General Supplies Put nearly 40 years of Agilent knowledge to work for your HPLC system Agilent offers a wide range of meticulously engineered supplies for operating and maintaining your HPLC system including e LC tubing Connectors Vials and caps Crimpers and decappers Sample preparation solutions Econofilters and more In this section we ll review these key components and help you make the right choices for your lab You can also visit www agilent com chem for a full selection of Agilent LC columns and to access our comprehensive chromatogram database As an application supervisor I m known for using the phrase Don t hesitate to challenge It means never look for the easiest safest route you might miss something along the way Instead do
46. indow 5 spring washers 2 seal rings Part No G1315 60015 79883 68703 79883 27101 1000 0953 79883 28802 5062 8553 79883 22404 G1315 87325 G1315 87306 G1315 84901 0515 1056 G1315 87312 G1315 87311 79883 68700 www agilent com chem DETECTORS Nano Flow Cells Description Part No 500nl Flow Cell for 1100 DAD MWD Contains quartz flow cell with 10mm path length and 500nl volume G1315 68724 connecting capillaries max 50 bar pressure 80nL Flow Cell for 1100 DAD MWD Contains quartz flow cell with 6mm path length and 80nl volume connecting G1315 68716 capillaries max 50 bar pressure Sealing kit for 500nl flow cell Includes torque adapter 2 cell seal assemblies 5 litetouch front and back ferrules also obra Sealing kit for 80nl flow cell Includes torque adapter 2 cell seal assemblies 5 litetouch front and back ferrules 5 G1315 68725 sleeves for 360um od capillaries Prep Flow Cells Description Part No Prep Flow Cell SST 3 mm 120 bar G1315 60016 Prep Flow Cell Quartz 0 3 mm 20 bar G1315 60017 Prep Flow Cell Quartz 0 06 mm 20 bar G1315 60018 www agilent com chem 47 DETECTORS LC MS Supplies LC MSD and LC MS Trap Supplies 48 Description Electron multiplier replacement horn EM Replacement Horn for the LC MSD Trap Rough pump oil 10T API ES Nebulizer needle APCI Nebulizer Needle Nebulizer needle ferrule Corona Needle APCI Cloth lint free 15 pk Abrasive mesh m
47. ing method is typically reproducible to 1 0 relative standard deviation RSD The complete filling method is reproducible to 0 1 RSD for 5 pl loops Dual mode or front loading injection which enables either the partial filling or the complete filling of the sample loop Series 77251 and 97251 Analytical Injection Valves Make Before Break MBB technology allows switching without flow interruption Stainless steel SS 77251 and PEEK 97251 valves are the most popular injection valves for analytical HPLC Features include e Wide 30 port angles offer A 20 pl loop installed Loops are also easier access to fittings available in stainless steel or PEEK from 5 ul to 5 ml 10 ml for PEEK Built in position sensing switch provides the chromatograph with a reproducible start signal www agilent com chem MANUAL INJECTION VALVES Series 37251 038 and 37251 Preparative Injection Valves The series 3725i 038 stainless steel and High reproducibility for both partial 37251 PEEK are the most suitable manual filling and complete filling methods valves for large sample volumes high flow rates and preparative columns sized 1 0 10 cm in diameter Sample range is 100 ul to 20 ml 10 ml loop is installed Versatile ports accommodate 1 8 Rg Ea gS te Ce sa 3 2 mm and 1 16 1 6 mm OD tubing Built in position sensing switch Note 1 16 OD tubing requires an gives the chromatograph a adapter
48. jection valve C G1329 87101 PEEK Coated Fused Silica Capillaries for use with 20 ul min Flow Range Item From To Fitting ID um Part No a EMPV Flowsensor B B 50 um 61375 87301 b Flowsensor Injection valve B C 50 um G1375 87310 c Injection valve Metering device B C 50 um G1375 87302 d Metering device Needle B B 100 um G1375 87303 e Injection valve Column C D 50 um G1375 87304 f Injection valve Waste C 100 um G1375 87307 g Column Detector D E 50 um G1315 68703 h Detector Waste E 75 um G1315 68708 switching valve Column C D 50 um G1375 87309 www agilent com chem 57 GENERAL SUPPLIES 58 Capillaries for Nano LC System Description Fused silica PEEK capillary 25 um 10cm Fused silica PEEK capillary 25 um 22cm Fused silica PEEK capillary 25um 35cm Fused silica PEEK capillary 25um 55cm Fused silica PEEK capillary 25um 70cm Fused silica PEEK capillary 50um 10cm Fused silica PEEK capillary 75um 65cm Nanoflow LC start up kit Standard Vials Description Crimp top vial Crimp top vial Crimp top vial Crimp top vial Crimp top vial Crimp top vial Crimp top vial Crimp top vial Crimp top vial 2ml Clear 2ml Clear 2ml Clear silanized 2ml Clear write on spot 2ml Clear write on spot 2ml Clear write on spot silanized 2ml Amber write on spot 2ml Amber write on spot 2ml Amber write on spot silanized Crimp snap top vial 1ml Polypropylene Crimp snap top vial 0 3m Polypropylene Crimp
49. ludes pump cassette and motor 4 seal keepers 4 support ring assemblies seal insert tool silicone tubing Peristaltic pump cassette with silicone tubing Parts and Supplies G1376A 1100 Series Capillary Pump Description Accessory kit for capillary pump Inline filter assembly with 0 5um pore size filter Replacement frits 0 5um for 5064 8273 10 pk Torque Adapter Bottle head assembly with tubing and filter Solvent inlet filter stainless steel www agilent com chem Part No G1376 68705 5064 8273 5022 2185 G1315 45003 G1376 60003 01018 60025 PUMP MAINTENANCE Part No 01018 68722 0890 1764 9001 3743 0905 1175 5062 2484 G1311 68711 G1312 68711 5042 8507 29 PUMP MAINTENANCE G1361A 1100 Series Prep Pump Description Part No O ring Viton 30mm 0905 1516 Glass filter solvent inlet 3150 0944 40 um pore size SS filter assembly with PEEK ring 5022 2192 2 um pore size Solvent bottle clear 2 L 2 inlets 5065 4421 Solvent bottle amber 2L 9301 6341 Solvent bottle clear 2L 9301 6342 Sapphire plunger for prep pump Frit adapter Peristaltic pump G1361 22402 G1361 23204 9065 4445 Valve assembly inlet outlet G1361 60012 for prep pump SS capillary 0 6x173 mm 2 1 16 male G1361 67300 SS capillary 0 6x175 mm 2 1 16 male G1361 67301 SS capillary 0 6x400 mm 2 1 16 male G1361 67302 SS capillary 0 5x160 mm 2 1 16 male G1361 67303 3
50. lumn back pressure and reduce efficiency Microbore column blockages are a particular problem due to the small diameter of the inlet frit To prevent blockages always use the appropriate filters in your LC system Agilent offers two types of high pressure in line filter kits for use with any HPLC system Universal solvent filter A high capacity filter with built in efficiency Universal solvent filters are installed between the LC pump and the injector so particles from the solvent can be removed before they reach the injector The filter assembly consists of a 4 8 mm frit two inserts and a two piece holder The frit is placed between the tapered edges of the inserts in such a way that the solvent is evenly distributed over the whole surface of the frit This provides efficient filtration and extends the life of the frit Low volume column inlet filter Ideal for microbore narrow bore high speed or standard analytical columns This low volume column inlet filter is positioned immediately before the LC column so it can remove particles from both the injection system and the sample With a frit diameter of only 2 1 mm plus tapered inserts this filter minimizes external bandspreading while maximizing the filtration capacity HPLC In line filters Description Part No Low dispersion in line filter Includes one 1 6mm 2um frit filter holder with inserts 01090 68702 60 x 0 12mm connecting capillary Replacement frits for 2 1m
51. lvent that you plan to use for your next application www agilent com chem Pump Head with Seal Wash Kit Used with standard and capillary pump The routine use of highly concentrated buffer solutions 100 mM will reduce the life of the seals and pistons in your 1100 Series pump But you can counteract the problem with one of our seal wash kits which flushes the backside of the seal with a wash solvent water isopropanol 90 10 is recommended as the wash solvent The continuous seal wash uses gravity to flow the wash solvent and is compatible with Description Continuous seal wash kit Includes 2 wash seal gaskets 4m flex tubing 2 pump seals 1 flow regulator 2 seal keepers all 1050 and 1100 standard and capillary pumps The active seal wash uses a peristaltic pump to flow the wash solvent and is compatible with 1100 pumps with the following serial numbers and higher e G1310A DE40906378 e G1311A DE40926032 G1312A DE40914884 2 support ring assemblies 20 mL luer lock syringe seal insert tool abrasive paper Silicone tubing 1mm ID 3m Seal Keeper Wash seal Wash seal gasket 6 pk Active seal wash kit for iso or quat pumps Includes 2 wash seal gaskets 2 pump seals peristaltic pump includes pump cassette and motor 2 seal keepers 2 support ring assemblies seal insert tool silicone tubing Active seal wash kit for binary pumps Includes 4 wash seal gaskets 4 pump seals 2 peristaltic pumps inc
52. m columns 2um 10 pk 280959 904 Replacement frits for 2 1mm columns 0 5um 10 pk 280959 907 Universal solvent filter kit Includes one 4 8mm 2um frit filter holder with inserts 01090 68703 130 x 0 25mm connecting capillary Replacement frits 4 8mm 2 pk 01090 27609 www agilent com chem 33 PUMP MAINTENANCE 34 Description Online degasser asscessory kit Includes 8 screws 8 bushings 4 markers tubing syringe syringe adapter Glass filter solvent inlet 20 um Disposable Syringes 20 mL 10 pk Syringe Adapter 1 16 in od 2 in long Tefzel Ferrules and SS Lock Rings 1 8in 10 pk PPS nuts 1 8 in 1 4 28 thread 10 pk Union 1 4 28 threads Polypropylene Teflon Solvent tubing Tubing kit degasser to pump Mounting tool for flangeless nut Plastic tubing cutter G1379A 1100 Series Micro Vacuum Degasser Description Dual channel micro degasser vacuum chamber Tubing kit micro vacuum degasser Mounting tool for flangeless nut Vacuum Degasser Parts and Supplies G1322A 1100 Series Vacuum Degasser Part No G1322 68705 5041 2168 5062 8534 9301 1337 0063 6598 9063 6599 5022 2155 5062 2483 G1322 67300 0100 1710 8710 1930 Part No G1379 60001 G1379 67300 0100 1710 og 5063 6598 ae 5063 6599 0100 1710 www agilent com chem Detector Maintenance Promoting optimal performance and functionality To maintain your HPLC System s level
53. o a quality lifecycle and are maintained under version and revision control Increased control Agilent will verify known elements and identify unknown network contingency states that may impact application performance Reduced regulatory exposure audit ready documentation establishes evidence of network system control Your staff can devote more time to chemistry not instrument maintenance Efficient cleaning with minimal downtime Fast and easy you simply Insert a disk reboot and you ll be back in operation in as little as two hours Normally you would have to reload and reconfigure your software a process that can take days A complete solution the initial configuration backup includes 65 SERVICES AND SUPPORT Education amp Training Services Highly trained lab professionals can boost your productivity minimize errors and reruns and expand your chromatography capabilities And that s why Agilent offers several training options that cover everything from troubleshooting and maintenance to the most advanced operational techniques e Learning Agilent s e Learning program features a series of focused economical and individualized instrument training modules designed to enhance and simplify your learning experience From theory and operations to common problems with your instruments eLearning Is available when you want it where you want It and how often you want it All you need is Int
54. ommitment to Your Lab for One Fixed Cost Agilent service agreements operate under a yearly fixed cost which includes preventative maintenance compliance telephone support repairs parts and labor No matter how many service visits or replacement parts you need this fixed cost covers it all at no additional charge The Unmatched Expertise of Fully Trained Engineers You can rest assured that the engineer who arrives at your site will perform the requested service with optimum skill That s because Agilent engineers Are experts in instrument operation maintenance compliance and repair Continually update their training and qualifications Use only Agilent factory approved replacement parts columns and supplies to ensure peak instrument reliability Carry calibrated traceable tools and testing equipment as well as Agilent hardware and software qualification protocols What s more most Agilent engineers are part of the Agilent product development team So you can be certain that ease of repair maintenance and compliance are built into your GC and GC MS systems right from the start The Flexibility to Select the Solution That s Right for You Our team will work with you to help your laboratory achieve Maximum uptime Verified system performance e High productivity The skills and knowledge you need for efficient operation and maintenance Total compliance Consistent in depth documentation
55. or visibly damaged Before replacing the capillary be sure to 1 Place the transport unit of the micro fraction collector spotter in the Home position 2 Remove all installed trays from the tray base 3 Place the transport unit in the Change Parts position 4 Turn off the instrument 5 Unscrew the micro fraction spotter capillary from the flow cell Simple Repairs of the detector Procedure When to Perform Replace fraction collector capillar Ra SNIS touts P ana or when worn blocked or damaged e nange tue Capitaty When bent or damaged guiding assembly When the flow delay sensor Exchange the internal tray no longer works properly Exchange the flap septum At least every six months and waste tubing Or when defective or contaminated 20 www agilent com chem G1364D Micro Fraction Collector Description MALDI spotting adapter for G1364D Pushing adapter assembly for G1364D Flap septum PEEK for internal tray Fused silica PEEK capillary 25 um 50 cm Fused silica PEEK capillary 50 um 50 cm Fused silica PEEK capillary 100 um 50 cm Waste tube PTFE 20cm 1 4mm ID 2 0mm od MALDI plate carrier Bruker MALDI plate carrier ABI MALDI plate carrier Agilent MALDI plate carrier Micromass Target Plate for AP MALDI LC MS Calibration Plate Bruker Calibration Plate ABI 10x10 amp 20x20 Calibration Plate Agilent Calibration Plate Micromass On line matrix Kit for MALDI sp
56. otting Includes BCD board cable syringe needles adapters connector capillary www agilent com chem Part No G1364 83205 G1364 60021 G1364 27107 G1364 87304 G1364 87305 G1364 87306 G1364 86711 5022 6541 5022 6542 5022 6543 5022 6544 G1972 60025 5023 0208 5023 0213 5023 0214 5023 0215 G1364 68706 FRACTION COLLECTORS G1364 83205 G1364 60021 5022 6541 5023 0208 21 Now there s a cleaner smarter way to maintain your LC MS rough pump Introducing the Agilent Quiet Cover Nobody likes changing the oil in an LC MS rough pump It s a heavy dirty job from lifting the pump out of its pan to holding the pump steady while the oil drains But with the Agilent Quiet Cover there s no sweating straining or spilling Need to check the oil Just open the Quiet Cover s door Need to add oil Simply lift the Quiet Cover s top to access the Till cap Need to change the oil Just slide out the drainage tray What s more the Quiet Cover fits over your rough pump in seconds and features A sound absorbing cabinet with mounting grommets to reduce noise and vibration Two internal fans to prevent overheating A built in alarm system that warns you if the temperature exceeds 35 C So order your Quiet Cover today and discover a cleaner smarter way to keep your pump running at peak efficiency Part 63199A Call 1 800 227 9770 option
57. pump head O1 Perform the seal wear in procedure Standard seals 5063 6589 only The wear in procedure involves flushing the seals with isopropanol at high pressure L while they are in place sA Description Unit Part No Sapphire Piston 5063 6586 Piston Seals graphite filled Teflon reversed phase 2 pk 5063 6589 Piston Seals polyethylene normal phase 2 pk 0905 1420 Piston housing assembly includes springs G1311 60002 Extended PM kit 1100 1050 pumps Contains 1ea 5063 6589 piston seal 01018 22707 PTFE frits 5 PK 5062 8562 cartridge active inlet valve G1311 60012 outlet viv amp 2 5063 6586 pistons 5065 4499 Changing and Inspecting the 1 Disassemble the pump head assembly Sapphire Pistons 2 Remove any deposits particularly buffers from the piston surface using lt All pumps except the alcohol or toothpaste Replace the G1361A preparative pump pistons if scratched Each time you replace the seals examine 3 Rinse thoroughly in alcohol the pistons for scratches which can cause and HPLC grade water small but damaging leaks 4 Reassemble the pump head 5 Check to make sure there are no fractures in the springs Replace piston housing assembly if springs are damaged 6 Reinsert the pistons www agilent com chem 21 PUMP MAINTENANCE 5 Close the purge valve and set the flow to a rate that is adequate to achieve a
58. ries 7725 Accessories Isolation Seal Rheodyne Series 7725 9725 3725 Rheotool socket wrench 1 4 inch Hex Key 9 64 15cm long T handle Ring stand mounting bracket PEEK adapter 1 8 to 1 16 4 pk Position sensor switch for manual valves Part No 0101 0623 0101 0620 0101 1255 0101 1233 0101 0624 0100 1859 0100 1860 1535 4046 8710 2391 8710 2394 1400 3166 9067 1503 0490 1849 www agilent com chem Sample Loops Stainless steel loop ends are square cut and burr free for a flush connection to the valve They are also supplied with unswaged fittings Flexible PEEK loop ends feature a clean straight cut for low dead volume connection Both types of sample loops are factory cut and finished to the highest quality Stainless Steel Sample Loops Remember size designations for loops are nominal The actual volumes can differ from their theoretical designations because of the 0 025 mm 0 001 tolerance of the metal tubing bore Generally accuracy rates are about 5 for large metal loops 1 0 mm 0 040 10 for intermediate loops 0 5 mm 0 020 and 30 for small loops 0 2 mm 0 007 However since both standards and unknowns are typically analyzed using the same sample loop knowledge of the actual volume Is rarely needed If you do need to know the sample volume it is best to calibrate the loop while it is on the valve so the valve s flow passage Is also t
59. rnational standards Verification that a system is performing at operation specifications after repair Complete system operation testing Note only a subset of the Operational Qualification test is performed based on the type and extent of the repair e A comprehensive test of the repaired module using established conditions and known sample characteristics to ensure the basic accuracy and precision of your module Measuring equipment is traceable to national and international standards Advantages to You Supplies the evidence you need to satisfy regulatory agencies No need to write your own SOPs to qualify Agilent instruments or to train your staff Consistent traceable results among all your laboratories and improved method transfer Confidence in the integrity of your measurements with traceable documented chains from sample introduction to reporting Less risk of financial loss due to noncompliance Meaningful relevant and understandable system tests Minimal system downtime after completion of instrument repair Efficient system requalification by performing the appropriate tests based on the components repaired Assurance that all instrument subsystems are performing within specifications All procedures and documentation meet regulatory agency requirements www agilent com chem Agilent Services Network Qualification Mass Spectrometer lon Source Cleaning For selected Agilent instr
60. rrules 10 pk 5180 4108 1 16 SS back ferrules 10 pk 5180 4114 Finger tight PEEK fittings beige 1 16 10 pk 5063 6591 0100 1631 Finger tight PEEK fittings beige 1 16 2 pk 0100 1516 Finger tight PEEK long fittings beige 1 16 10 pk 5062 8541 Finger tight PEEK fittings 1 16 as 2 each yellow blue black green red RS pasa Double winged 2 piece fittings 1 16 10 pk 5042 6500 PEEK RheFlex 2 piece fittings 5 pk 0100 1631 PEEK RheFlex 2 piece fittings 10 pk 0100 2175 colored Chromtrac identifiers 2 each black green white 20 pk 0350 1402 4 each yellow blue red Blanking Nut 1 16 SS 01080 83202 Unions Used In Description instrument Part No 0100 0900 True ZDV Union no fittings Nano LC 5022 2145 3 oe Capillary Nano ZDV Union no fitting Standard LC 5022 2184 da ZDV Union with fittings Standard LC 0100 0900 High flow union no fittings Prep LC 5022 2133 52 www agilent com chem Universal Connecting Capillaries Made of flexible stainless steel 0 6 mm OD with 1 16 fittings for both ends Pre swaged fittings are assembled according to Swagelok specifications Squarely cut pre cleaned and ready to use Use with SS fittings and ferrules 5062 2418 or PEEK fittings 0100 1516 Non swaged capillaries include fittings but not assembled Flexible Stainless Steel Capillaries without fittings ID mm 0 12 0 12 0 12 0 12 0 12 0 12 0 17 0 1
61. rst we ask customers what they would like to accomplish Then we recommend the best products for their needs and explain how to use them People come to Agilent because they know they ll get intelligent information that will help them be more productive How do know was once an Agilent customer Maureen Joseph Analytical Chemistry Applications Chemist AUTOSAMPLER MAINTENANCE Injection Valve Maintenance Rotor seal replacement is the most common injection valve Tools maintenance procedure Indications include rotor seal blockages and cross port leaks You should suspect a cross port leak if you notice a drop of mobile phase coming out of the needle when it rises to draw sample from a vial Or if you experience poor Metric hexagonal keys for the rest of the Autosampler screws injection volume precision Cross port leaks can also be found by performing a pressure test 9 64 Allen wrench to remove the stator face and disassemble the valve 1 4 Wrench to remove the capillaries Replacing the Rotor Seal For Standard and Well Plate Autosamplers G1313A G1327A G1367A 1 Remove the capillaries 4 Install the stator head from the stator face then the stator face Remove the stator Replace the stator screws with a 9 64 lt screws tightening them Allen wrench evenly and carefully O1 Connect all capillaries according to the diagram below 2 Remove the stator head stator face and s
62. s temperature exposure time and concentration of organic solvents can all impact durability Additionally solvents such as THF methylene chloride and DMSO cause PEEK to swell while concentrated nitric acid and sulfuric acid can weaken PEEK tubing PEEK Sample Loops for 9725 Injectors Description 5 ul 10 pl 20 ul 50 ul 100 ul 200 ul 500 ul 1 ml 2 ml 5 ml PEEK Sample Loops for 3725 Injectors Description 2 ml 5 ml 10 ml 20 ml ID mm 0 18 0 25 0 25 0 51 0 51 0 51 0 76 0 76 0 76 0 76 ID mm 1 6 1 6 2 0 2 0 Part No 0101 1241 0101 1240 0101 1239 0101 1238 0101 1242 0101 1237 0101 1236 0101 1235 0101 1234 0101 1230 Part No 0101 1229 0101 1228 0101 1227 0101 1226 www agilent com chem www agilent com chem Autosampler Maintenance Ensuring peak performance and increasing your lab s uptime Routine maintenance of your Autosampler should include the following tasks Replacing the rotor seal Checking the stator Replacing the needle and needle seat e Checking the finger caps Replacing the metering unit seal Cleaning the piston Cleaning the solvent waste path e Checking the leak sensor e Performing a pressure test This section will give you step by step instructions on how to perform many of these procedures You ll also find the top quality Agilent consumables and accessories you need to keep your Autosampler running at top efficiency Fi
63. s and 11mm crimp caps with Black amp Decker VersaPak Gold battery and charger 5184 3567 Electronic Crimper for 20mm crimp vials and 20mm aluminum crimp caps with Black amp Decker VersaPak Gold battery and charger 5184 3572 Electronic Decapper for 20mm crimp vials and 20mm aluminum crimp caps with Black amp Decker VersaPak Gold battery and charger 5184 3573 Black and Decker VersaPak Gold NiMH rechargeable battery 5183 4799 www agilent com chem www agilent com chem Services and Support Every Agilent LC and LC MS system is backed by our nearly 40 years of experience with instrument design lab operations business processes and regulatory requirements We stand behind our products with Immediate technical assistance by phone or online Prompt onsite service from Agilent certified Engineers e A variety of service options including maintenance repair compliance and consultation e Industry leading training courses to help increase your lab s overall knowledge base Our professionals are standing by to help you solve problems and optimize your resources So you can spend more time running samples developing methods and meeting production deadlines and less time arranging for instrument services Our customers know they can rely on Agilent s expertise and resources to keep their instruments running at top performance Yukiko Sameshima Customer Engineer 61 SERVICES AND SUPPORT A Total C
64. tator ring Pump Metering device Plug Waste Needle seat Column mooo D gt 3 Remove and replace the rotor seal and iA the isolation seal an if necessary o a www agilent com chem Injection Valve Maintenance Used In instrument Description Part No G1313A 13294 2 Pos 6 Port 0101 0921 Injection Valve G1367A Bee e E Fan 0101 1050 u Injection Valve G225gq 10 Port Dual 0101 1385 Loop Valve 2 Position 6 Port CADUA MBB Injection Valve uiia RheBuild Kit Part Rotor Seal No Part No 0100 1853 Vespel 0100 1849 0101 1257 Tetel 0100 2231 PEEK 0100 2088 0101 1257 festa 0100 2415 0101 1268 Exchanging the Needle Needle Seat for the G1313A G1329A G1389A and G2260A See manual for G1367A and G1377A The needle should be replaced when it becomes bent burred or blunt The needle seat should be replaced when it is leaking or plugged You should suspect a leak if you notice a trail of buffer crystals on the needle seat The needle seat can become blocked if the sample contains particulates as this is the first restriction that the sample experiences If this occurs try backflushing the needle seat capillary Exchanging the Needle Before beginning this procedure e Select Change Needle under Maintenance Function Remove the front cover when the needle is positioned approximately 15 mm over the nee
65. tubing accepts both stainless steel and PEEK fittings To ensure leak free connections and to prevent the loss of peak shape and resolution always use the manufacturer s recommended fittings with columns valves and unions Additionally keep the following tips in mind Different columns have different fitting requirements For example Zorbax columns use standard Swagelok fittings When should replace the LC tubing Agilent 1100 modules use standard Swagelok fittings Rheodyne injection valves require Rheodyne fittings You should inspect your system periodically and replace the tubing when you notice Leaking or loose fittings Pinched or constricted tubing Application changes PEEK Tubing Length m ID mm Color Code Part No 1 5 0 50 Orange 0890 1761 1 5 0 25 Blue 0890 1762 5 0 25 Blue 5042 6463 15 0 18 Yellow 0890 1763 5 0 18 Yellow 5042 6462 1 5 0 13 Red 0890 1915 5 0 13 Red 5042 6461 Description Part No Plastic tubing cutter 8710 1930 Blades for tubing cutter 5 PK 8710 1931 8710 1930 www agilent com chem 5 GENERAL SUPPLIES Standard Fittings for 1 16 od Capillaries 095099 0350 1402 1 16 SS fittings front and back ferrules Description Unit Part No 10 pk 5062 2418 1 16 SS long fittings front and back ferrules 10 pk 5065 4454 Extra long SS fittings and ferrules 10 pk 5065 9967 padi 1 16 SS male fittings 10 pk 5061 3303 1 16 SS front fe
66. uments Software Backup www agilent com chem Features Network definition and performance testing using established Agilent Design Installation Qualification and Operational Qualification protocols Documentation of the definition integrity Supportability and health of high risk network segments Completed protocols created using hardware and software network testing tools Protocols include A physical and logical inventory A graphical network topology map A snapshot of network health as installed Network Operational Qualification testing which monitors the network over time and evaluates its operation over a controlled range of traffic conditions We ll also provide the following reports Long Duration Network Characterization which analyzes each function in its operating environment Performance Predictability Analysis which documents reserve capacity and stability over a range of operating demands Onsite disassembling e Cleaning Reassembling Testing Telephone or onsite factory support to facilitate restoration Captures the unique system settings for all software components needed to reconstruct your system including SERVICES AND SUPPORT Advantages to You Significant time savings Increased uptime issues that might impact application performance are quickly identified and resolved Improved consistency network qualification protocols are developed according t
67. ur hands 2140 0820 Sg Tighten the screws and reconnect the lamp to its electrical connection o Replace the front cover N Reset the lamp counter and turn on the lamp Allow it to warm up for 10 20 minutes Note The new C type c a Agilent s 1100 series DAD lamp can help detectors use lamps with a chip installed i to automatically count used lamp time you achieve optimal performance with these innovative features A lifetime of over 2000 hours e Precise alignment e Thermal stability e Easier handling during installation and removal 42 www agilent com chem DETECTORS Choosing the Optimal Flow Cell If more than one selection is appropriate path length also increases the cell volume choose the larger flow cell to get the best which causes more peak dispersion Agilent offers eight flow cells for use with detection limit the diode array and multiple wavelength detectors Use the chart below to select the appropriate one for your application keeping the following guidelines in mind For information on selecting prep cells For columns capable of providing narrower please refer to our technical note peak widths 1 8 um particle size the G1315 90102 under Library Information smaller flow cell may be more appropriate at www agilent com chem Flow cells with longer path lengths provide better signal detection However a longer DAD MWD Flow Cell Selection Typical Column Typical Peak
68. ust 1 2005 5989 2662EN
69. w in air instead of solvent This will result in periodic baseline disturbances Filter solvents through sterile filters lt 1 um Avoid exposure to direct sunlight or use brown glass bottles Solvent Filter Degasser An added benefit of filtering solvents Is Increases solvent inlet lifetime that they are degassed at the same time This is particularly beneficial if you do not have an on line degasser in your system The benefits of solvent filtration Decreases piston wear while increasing column life Eliminates pump downtime caused by air locks and particulates in check valves Degasses eluents as particulates are removed Prevents the formation of spurious peaks within the detector due to solvent outgassing at the low pressure end of the chromatograph 3150 0577 Description Unit Part No HPLC Solvent filter degasser assembly 3150 0577 Regenerated Cellulose Filter Membranes 47 mm pore size 0 45 um 100 pk 3150 0576 Nylon Filter Membranes 47 mm pore size 0 45 um PTFE Filter Membranes 47 mm pore size 0 45 um 100 pk 9301 0895 10 pk 3150 0509 Glass funnel 250ml for 3150 0577 5188 2743 PTFE Coated sieve for 3150 0577 5188 2744 PTFE seal for 3150 0577 5188 2745 Funnel base glass for 3150 0577 5188 2746 www agilent com chem 31 Cleaning or Replacing the Solvent Inlet Filters Clean or replace the solvent inlet filters at least once every 3 months Stainless steel filters c

Maintaining Your Agilent 1100 Series HPLC System

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