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Agilent 1260 Infinity Binary Pump VL User Manual

1. Agilent Technologies Notices Agilent Technologies Inc 2008 2010 2012 No part of this manual may be reproduced in any form or by any means including elec tronic storage and retrieval or translation into a foreign language without prior agree ment and written consent from Agilent Technologies Inc as governed by United States and international copyright laws Manual Part Number 61312 90008 Edition 02 2012 Printed in Germany Agilent Technologies Hewlett Packard Strasse 8 76337 Waldbronn This product may be used as a com ponent of an in vitro diagnostic sys tem if the system is registered with the appropriate authorities and com plies with the relevant regulations Otherwise it is intended only for gen eral laboratory use Warranty The material contained in this docu ment is provided as is and is sub ject to being changed without notice in future editions Further to the max imum extent permitted by applicable law Agilent disclaims all warranties either express or implied with regard to this manual and any information contained herein including but not limited to the implied warranties of merchantability and fitness for a par ticular purpose Agilent shall not be liable for errors or for incidental or consequential damages in connection with the furnishing use or perfor mance of this document or of any information contained herein Should Agilent and the user have a separate
2. Exchange the outlet valve Exchange damper 121 122 Test Functions and Calibration First plateau negative or unstable and at least one other plateau positive Probable cause 1 Leaking outlet valve in channel A 2 Loose pump head screws in channel A 3 Leaking seal or scratched piston in channel A2 Suggested actions Clean the outlet valve in channel A Ensure the sieve in the outlet valves are installed correctly Tighten the outlet valve Ensure the pump head screws in channel A are tight Exchange the pump seals in channel A Check the piston for scratches Exchange if scratched Second plateau negative or unstable and at least one other plateau positive Probable cause 1 Leaking outlet valve in channel B 2 Loose pump head screws in channel B 3 Leaking seal or scratched piston in channel B2 Suggested actions Clean the outlet valve in channel B Ensure the sieve in the outlet valves are installed correctly Tighten the outlet valve Ensure the pump head screws in channel B are tight Exchange the pump seals in channel B Check the piston for scratches Exchange if scratched 1260 Binary Pump VL User Manual Test Functions and Calibration 8 Third plateau negative or unstable and at least one other plateau positive Probable cause 1 Airin channel A or new seals not yet seated 2 Loose active inlet valve in channel A 3 Loose pump head screws in channel A 4 Loose outlet val
3. 7 Error Information Pressure Above Upper Limit Error ID 2014 2500 The system pressure has exceeded the upper pressure limit Probable cause Suggested actions 1 Upper pressure limit set too low Ensure the upper pressure limit is set to a value suitable for the analysis 2 Blockage in the flowpath after the damper Check for blockage in the flowpath The following components are particularly subject to blockage inline filter frit needle autosampler seat capillary autosampler sample loop autosampler column frits and capillaries with small internal diameters e g 50 pm ID 3 Defective damper Please contact your Agilent service representative 4 Defective main board Please contact your Agilent service representative 94 1260 Binary Pump VL User Manual Pressure Below Lower Limit Error ID 2015 2501 Error Information The system pressure has fallen below the lower pressure limit Probable cause Suggested actions 1 Lower pressure limit set too high Ensure the lower pressure limit is set to a value suitable for the analysis 2 Air bubbles in the mobile phase Make sure that the degasser is in flow path and works correctly Purge the module Ensure solvent inlet filters are not blocked 3 Leak Inspect the pump head capillaries and fittings for signs of a leak Purge the module Run a pressure test to determine whether the seals or other module components are defective 4 Defective dam
4. Analog Cables 190 This chapter provides information on cables used with the Agilent 1200 Infinity Series modules ee Agilent Technologies 177 11 Identifying Cables Cable Overview 178 Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Analog cables p n Description 35900 60750 Agilent module to 3394 6 integrators 35900 60750 Agilent 35900A A D converter 01046 60105 Analog cable BNC to general purpose spade lugs Remote cables p n Description 03394 60600 Agilent module to 3396A Series integrators 3396 Series Il 3395A integrator see details in section Remote Cables on page 182 03396 61010 Agilent module to 3396 Series III 3395B integrators 5061 3378 Remote Cable 01046 60201 Agilent module to general purpose BCD cables p n Description 03396 60560 Agilent module to 3396 integrators G1351 81600 Agilent module to general purpose 1260 Binary Pump VL User Manual Identifying Cables 11 CAN cables p n Description 5181 1516 CAN cable Agilent module to module 0 5 m 5181 1519 CAN cable Agilent module to module 1 m LAN cables p n Description 5023 0203 Cross over network cable shielded 3 m for point to point connection 5023 0202 Twisted pair network cable shielded 7 m for point to point connection External Contact Cable p n Description G1103 61611 External contact cable
5. Solvent cabinet Vacuum degasser Instant Pilot Autosampler Column compartment Detector Figure 4 Recommended Stack Configuration for 1260 Infinity Front View 32 1260 Binary Pump VL User Manual Installing the Pump 3 Optimizing the Stack Configuration TTT TTT Remote cable CAN Bus cable to Instant Pilot AC power CAN Bus cable Analog detector signal 1 or 2 outputs per detector LAN to LC ChemStation location depends on detector Figure 5 Recommended Stack Configuration for 1260 Infinity Rear View 1260 Binary Pump VL User Manual 33 3 Installing the Pump Optimizing the Stack Configuration Two Stack Configuration To avoid excessive height of the stack when the autosampler thermostat is added to the system it is recommended to form two stacks Some users prefer the lower height of this arrangement even without the autosampler thermostat A slightly longer capillary is required between the pump and autosampler See Figure 6 on page 34 and Figure 7 on page 35 Instant Pilot DE _ ___ Detector lel iy Column compartment flrs Solvent cabinet Degasser optional Autosampler hermostat for the ALS ppnow Figure 6 Recommended Two Stack Configuration for 1260 Infinity Front View 34 1260 Binary Pump VL User Manual Installing the Pump 3 Optimizing the Stack Configuration LAN
6. Seal holder Seal wash support ring Piston housing 2 SS Piston The best way to inspect a piston is to hold it up and watch e g a light bulb through the piston rod The transparent sapphire acts as a very strong magnifier and even smallest surface abnormalities become visible 142 1260 Binary Pump VL User Manual Maintenance Maintenance of a Pump Head with Seal Wash 4 Using the steel side of the insert tool carefully remove the seal from the pump housing Remove wear retainers if still present Insert tool Piston seal Pump housing 5 Using the plastic side of the insert tool insert the new seals into the pump head and press them firmly in position Insert tool SES ee o Piston seals y Q Fay b SY NG Ps le X 6 Using the steel side of the insert tool remove the seal wash gasket and the wash seal from the support ring The removed seal will be damaged and cannot be re used Wash seal 7 Using the plastic side of the insert tool press the new wash seal spring pointing upwards into the recess of the support ring Insert tool Wash seal Support ring 1260 Binary Pump VL User Manual 143 9 Maintenance Maintenance of a Pump Head with Seal Wash 8 Place a seal wash gasket in the recess of the support ring Put the seal holder on top of the gasket Seal holder Seal wash gasket Support ring 9 Place the support rings on th
7. The module power supply has wide ranging capability It accepts any line voltage in the range described in Table 2 on page 23 Consequently there is no voltage selector in the rear of the module There are also no externally accessible fuses because automatic electronic fuses are implemented in the power supply WARNING Hazard of electrical shock or damage of your instrumentation can result if the devices are connected to a line voltage higher than specified gt Connect your instrument to the specified line voltage only WARNING The module is partially energized when switched off as long as the power cord is plugged in Repair work at the module can lead to personal injuries e g electrical shock when the cover is opened and the module is connected to power gt Always unplug the power cable before opening the cover gt Do not connect the power cable to the instrument while the covers are removed 20 1260 Binary Pump VL User Manual CAUTION Site Requirements and Specifications 2 Inaccessible power plug In case of emergency it must be possible to disconnect the instrument from the power line at any time gt Make sure the power connector of the instrument can be easily reached and unplugged gt Provide sufficient space behind the power socket of the instrument to unplug the cable Power Cords Different power cords are offered as options with the module The female end of all power cords is
8. Agilent module interface board to general purposes RS 232 cables p n Description G1530 60600 RS 232 cable 2m RS232 61600 RS 232 cable 2 5m Instrument to PC 9 to 9 pin female This cable has special pin out and is not compatible with connecting printers and plotters It s also called Null Modem Cable with full handshaking where the wiring is made between pins 1 1 2 3 3 2 4 6 5 5 6 4 7 8 8 7 9 9 5181 1561 RS 232 cable 8m 1260 Binary Pump VL User Manual 179 11 Identifying Cables Analog Cables 180 IEIO One end of these cables provides a BNC connector to be connected to Agilent modules The other end depends on the instrument to which connection is being made Agilent Module to 3394 6 Integrators p n 35900 60750 Pin 3394 6 Pin Agilent Signal Name module 1 Not connected 2 Shield Analog 3 Center Analog To oO 20 Agilent Module to BNC Connector p n 8120 1840 Pin BNC Pin Agilent Signal Name module ef O Shield Shield Analog ad Center Center Analog 1260 Binary Pump VL User Manual Agilent Module to General Purpose Identifying Cables 11 p n 01046 60105 Pin Pin Agilent Signal Name module 1 Not connected 2 Black Analog 3 Red Analog _ a 1260 Binary Pump VL User Manual 181 11 Identifying Cables Remote Cables 182 One end of these cables provides a Agilent Technolog
9. written agreement with warranty terms covering the material in this document that conflict with these terms the warranty terms in the sep arate agreement shall control Technology Licenses The hardware and or software described in this document are furnished under a license and may be used or copied only in accor dance with the terms of such license Restricted Rights Legend If software is for use in the performance of a U S Government prime contract or subcon tract Software is delivered and licensed as Commercial computer software as defined in DFAR 252 227 7014 June 1995 or as a commercial item as defined in FAR 2 101 a or as Restricted computer soft ware as defined in FAR 52 227 19 June 1987 or any equivalent agency regulation or contract clause Use duplication or dis closure of Software is subject to Agilent Technologies standard commercial license terms and non DOD Departments and Agencies of the U S Government will receive no greater than Restricted Rights as defined in FAR 52 227 19 c 1 2 June 1987 U S Government users will receive no greater than Limited Rights as defined in FAR 52 227 14 June 1987 or DFAR 252 227 7015 b 2 November 1995 as applicable in any technical data Safety Notices CAUTION A CAUTION notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly per formed or adhered to could result i
10. 0 1 1 1 Parity No Parity Odd Parity Even Parity One start bit and one stop bit are always used not selectable Per default the module will turn into 19200 baud 8 data bit with no parity 1260 Binary Pump VL User Manual Hardware Information 12 Special Settings The special settings are required for specific actions normally in a service case Boot Resident Firmware update procedures may require this mode in case of firmware loading errors main firmware part If you use the following switch settings and power the instrument up again the instrument firmware stays in the resident mode It is not operable as a module It only uses basic functions of the operating system for example for communication In this mode the main firmware can be loaded using update utilities Table 19 Boot Resident Settings without on board LAN Mode Select sw1 sw2 sw3 SW4 SW5 SW6 SW7 sws TEST BOOT 1 1 0 0 1 0 0 0 Forced Cold Start A forced cold start can be used to bring the module into a defined mode with default parameter settings CAUTION Loss of data Forced cold start erases all methods and data stored in the non volatile memory Exceptions are calibration settings diagnosis and repair log books which will not be erased gt Save your methods and data before executing a forced cold start If you use the following switch settings and power the instrument up again a forced cold start has been
11. 213 13 Appendix Batteries Information Lithium batteries may not be disposed off into the domestic waste Transportation of discharged Lithium batteries through carriers regulated by IATA ICAO ADR RID IMDG is not allowed Danger of explosion if battery is incorrectly replaced gt Discharged Lithium batteries shall be disposed off locally according to national waste disposal regulations for batteries gt Replace only with the same or equivalent type recommended by the equipment manufacturer Lithiumbatteri Eksplosionsfare ved fejlagtig handtering Udskiftning ma kun ske med batteri af samme fabrikat og type gt Lever det brugte batteri tilbage til leverand ren Lithiumbatteri Eksplosionsfare Ved udskiftning benyttes kun batteri som anbefalt av apparatfabrikanten gt Brukt batteri returneres appararleverandoren 214 Bij dit apparaat zijn batterijen geleverd Wanneer deze leeg zijn moet u ze niet weggooien maar inleveren als KCA 1260 Binary Pump VL User Manual Appendix 13 Radio Interference Cables supplied by Agilent Technologies are screened to provide optimized protection against radio interference All cables are in compliance with safety or EMC regulations Test and Measurement If test and measurement equipment is operated with unscreened cables or used for measurements on open set ups the user has to assure that under operating conditions the radio interference limits are sti
12. A series of test functions are available for troubleshooting and operational verification after exchanging internal components see Tests and Calibrations 76 1260 Binary Pump VL User Manual Troubleshooting and Diagnostics 6 Pressure Test The Pressure Test is a quick test designed to determine the pressure tightness of the system i e the high pressure flow path between pump and column After exchanging flow path components e g pump seals or injection seal use this test to verify the system is pressure tight see Pressure Test on page 112 Leak Test The Leak Test is a diagnostic test designed to determine the pressure tightness of the pump components When a problem with the pump is suspected use this test to help troubleshoot the pump and its pumping performance see Leak Test on page 117 1260 Binary Pump VL User Manual 77 6 Troubleshooting and Diagnostics Status Indicators Two status indicators are located on the front of the module The lower left indicates the power supply status the upper right indicates the module status Status Indicator Power switch Serial number Figure 14 Location of Status Indicators Power Supply Indicator The power supply indicator is integrated into the main power switch When the indicator is illuminated green the power is ON 78 1260 Binary Pump VL User Manual Troubleshooting and Diagnostics 6 Module Status Indicator The module
13. Gold SST For pump specifications see Performance Specifications on page 24 How Does Compressibility Compensation Work The compressibility of the solvents in use will affect retention time stability when the back pressure in the system changes for example ageing of column In order to minimize this effect the pump provides a compressibility compensation feature which optimizes the flow stability according to the solvent type The compressibility compensation is set to a default value and can be changed through the user interface Without a compressibility compensation the following will happen during a stroke of the first piston The pressure in the piston chamber increases and the volume in the chamber will be compressed depending on backpressure and solvent type The volume displaced into the system will be reduced by the compressed volume With a compressibility value set the processor calculates a compensation volume that is depending on the backpressure in the system and the selected compressibility This compensation volume will be added to the normal stroke volume and compensates the previous described loss of volume during the delivery stroke of the first piston 1260 Binary Pump VL User Manual 15 1 16 Introduction How Does Variable Stroke Volume Work Due to the compression of the pump chamber volume each piston stroke of the pump will generate a small pressure pulsation influencing the flow stability
14. Pump VL User Manual 39 3 40 Installing the Pump Connecting an Agilent 1260 Infinity Vacuum Degasser 1 Place the vacuum degasser in the stack of modules as shown in Figure 4 on page 32 2 Ensure the power switch at the front of the vacuum degasser is OFF switch stands out 3 Plug an APG cable into the APG remote connector at the rear of the degasser 4 Connect the APG cable to the APG remote connector of the pump see Figure 5 on page 33 5 Press in the power switch to turn on the vacuum degasser The AUX output is intended for troubleshooting It provides a DC voltage in the range of 0 1 V which is proportional to the vacuum level in the degasser chambers 1260 Binary Pump VL User Manual Installing the Pump 3 Connecting Control Software and or G4208 A Instant Pilot With the introduction of the Agilent 1260 Infinity all GPIB interfaces have been removed The preferred communication is LAN Usually the detector is producing the most data in the stack followed by the pump and it is therefore highly recommended to use either of these modules for the LAN connection 1 Ensure the power switches on the front of the modules in the stack are OFF switches stand out 2 If there are no other 1260 with LAN port in the HPLC stack install a G1369B LAN board into the extension slot of the pump 3 Connect the LAN enabled module with a LAN cable to the data system 4 Plug the CAN connector of the Instant Pilot into
15. RX 2 o 2 RX TX 3 A 3 TX DTR 4 a 4 DTR GND 5 y 5 GND DSR 6 6 DSR RTS 7 X 7 RTS CTS 8 8 CTS RI 9 9 RI DB9 DB9 DB9 DB9 Male Female Female Male Figure 28 RS 232 Cable Analog Signal Output The analog signal output can be distributed to a recording device For details refer to the description of the module s main board 1260 Binary Pump VL User Manual 201 12 Hardware Information 202 APG Remote The APG Remote connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features as common shut down prepare and so on Remote control allows easy connection between single instruments or systems to ensure coordinated analysis with simple coupling requirements The subminiature D connector is used The module provides one remote connector which is inputs outputs wired or technique To provide maximum safety within a distributed analysis system one line is dedicated to SHUT DOWN the system s critical parts in case any module detects a serious problem To detect whether all participating modules are switched on or properly powered one line is defined to summarize the POWER ON state of all connected modules Control of analysis is maintained by signal readiness READY for next analysis followed by START of run and optional STOP of run triggered on the respective lines In addition PREPARE and START REQUEST may be issued The signal levels are defined
16. Select 1 2 3 4 5 6 7 8 RS 232C 0 1 Baudrate Data Parity Bits Reserved 1 0 Reserved TEST BOOT 1 1 RSVD SYS RSVD RSVD FC The LAN settings are done on the LAN Interface Card G1369B C Refer to the documentation provided with the card Communication Settings for RS 232C The communication protocol used in the column compartment supports only hardware handshake CTS RTR Switches 1 in down and 2 in up position define that the RS 232C parameters will be changed Once the change has been completed the column instrument must be powered up again in order to store the values in the non volatile memory Table 15 Communication Settings for RS 232C Communication without on board LAN Mode Select 1 2 3 4 5 6 7 8 Rs 232c 0 1 Baudrate Data Bits Parity Use the following tables for selecting the setting which you want to use for RS 232C communication The number 0 means that the switch is down and 1 means that the switch is up 1260 Binary Pump VL User Manual 205 12 Hardware Information 206 Table 16 Baudrate Settings without on board LAN Switches Baud Rate Switches Baud Rate 3 4 3 4 5 0 0 9600 1 0 0 9600 0 0 1200 1 0 1 14400 0 1 2400 1 1 0 19200 0 1 4800 1 1 1 38400 Table 17 Data Bit Settings without on board LAN Switch 6 Data Word Size 0 1 7 Bit Communication 8 Bit Communication Table 18 Parity Settings without on board LAN Switches 7 8 0 0
17. above the upper limit the error message is generated Probable cause Suggested actions 1 Defective main board Please contact your Agilent service representative Compensation Sensor Short Error ID 0080 The ambient compensation sensor NTC on the main board in the module has failed short circuit The resistance across the temperature compensation sensor NTC on the main board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor falls below the lower limit the error message is generated Probable cause Suggested actions 1 Defective main board Please contact your Agilent service representative 1260 Binary Pump VL User Manual Fan Failed Error ID 0068 Error Information The cooling fan in the module has failed 7 The hall sensor on the fan shaft is used by the main board to monitor the fan speed If the fan speed falls below a certain limit for a certain length of time the error message is generated This limit is given by 2 revolutions second for longer than 5 seconds Depending on the module assemblies e g the lamp in the detector are turned off to assure that the module does not overheat inside Probable cause 1 Fan cable disconnected 2 Defective fan 3 Defective main board 4 Improperly positioned cables or wires obstructing fan blades 1260 Binary Pump VL User M
18. actions how to recover from error conditions 1260 Binary Pump VL User Manual 3 In This Guide 8 Test Functions and Calibration This chapter describes the tests for the module 9 Maintenance This chapter describes the maintenance of the module 10 Parts for Maintenance This chapter provides information on parts for maintenance 11 Identifying Cables This chapter provides information on cables used with the Agilent 1200 Infinity Series modules 12 Hardware Information This chapter describes the pump in more detail on hardware and electronics 13 Appendix This chapter provides addition information on safety legal and web 4 1260 Binary Pump VL User Manual Contents Contents 1 Introduction 9 Introduction to the Binary Pump 10 Overview of the Binary Pump 11 Early Maintenance Feedback 17 Instrument Layout 18 2 Site Requirements and Specifications 19 Site Requirements 20 Physical Specifications 23 Performance Specifications 24 3 Installing the Pump 27 Unpacking the Binary Pump 28 Optimizing the Stack Configuration 31 Installing the Binary Pump 36 Connecting Modules and Control Software 39 Flow Connections of the Binary Pump with Optional Solvent Selection Valve 42 Flow Connections of the Binary Pump Without Solvent Selection Valve 45 Priming the System 48 4 Using the Binary Pump 53 Hints for Successful Use of the Binary Pump 54 Setting up the Pump with the G4208A Instant Pilot 55 Sett
19. amp Diagnostic Software 1 Select the pressure test from the test selection menu 2 Start the test and follow the instructions Make sure to release the pressure by slowly opening the purge valve when the test has finished System Pressure Test failed on page 116 describes the evaluation and interpretation of the pressure test results For detailed instructions refer to the Agilent Lab Monitor amp Diagnostic Software 1260 Binary Pump VL User Manual Test Functions and Calibration 8 Evaluating the Results The sum of all leaks between the pump and the blank nut will be indicated by a pressure drop of gt 2 bar minute at the plateau Note that small leaks may cause the test to fail but solvent may not be seen leaking from a module Please notice the difference between an error in the test and a failure of the test An error means that during the operation of the test there was an abnormal termination If a test failed this means that the results of the test were not within the specified limits If the pressure test fails e Ensure all fittings between the pump and the blank nut are tight Repeat the pressure test Often it is only a damaged blank nut itself poorly shaped from overtightening that causes a failure of the test Before investigating any other possible sources of failure make sure that the blank nut you are using is in good condition and properly tightened e Ifthe test fails again insert the blank nut
20. are out of range The values supplied to the ADC by the hybrid sensors must be between 0 5 V and 4 3 V If the values are outside this range the error message is generated Probable cause Suggested actions 1 Defective main board Please contact your Agilent service representative 1260 Binary Pump VL User Manual 99 7 Error Information Temperature Limit Exceeded Error ID 2517 Temperature Limit Exceeded 0 Pump channel A Temperature Limit Exceeded 1 Pump channel B The temperature of one of the motor drive circuits is too high The processor continually monitors the temperature of the drive circuits on the main board If excessive current is being drawn for long periods the temperature of the circuits increases If the temperature exceeds the upper limit the error message is generated Probable cause Suggested actions 1 High friction partial mechanical blockage in Remove the pump head assembly Ensure there the pump drive assembly is no mechanical blockage of the pump head assembly or pump drive assembly 2 Partial blockage of the flowpath in front of Ensure the outlet ball valve is not blocked the damper 3 Defective pump drive assembly Please contact your Agilent service representative 4 Defective main board Please contact your Agilent service representative 100 1260 Binary Pump VL User Manual Error Information 7 Motor Drive Power Error ID 2041 2042 Motor Drive Power Pump channel A B Motor Dri
21. as e standard TTL levels 0 V is logic true 5 0 V is false e fan out is 10 e input load is 2 2 kOhm against 5 0 V and e output are open collector type inputs outputs wired or technique All common TTL circuits operate with a 5 V power supply A TTL signal is defined as low or L when between 0 V and 0 8 V and high or H when between 2 0 V and 5 0 V with respect to the ground terminal 1260 Binary Pump VL User Manual Hardware Information 12 Table 13 Remote Signal Distribution Pin Signal Description 1 DGND Digital ground 2 PREPARE L Request to prepare for analysis for example calibration detector lamp on Receiver is any module performing pre analysis activities 3 START L Request to start run timetable Receiver is any module performing run time controlled activities 4 SHUT DOWN L System has serious problem for example leak stops pump Receiver is any module capable to reduce safety risk 5 Not used 6 POWER ON H All modules connected to system are switched on Receiver is any module relying on operation of others 7 READY H System is ready for next analysis Receiver is any sequence controller 8 STOP L Request to reach system ready state as soon as possible for example stop run abort or finish and stop injection Receiver is any module performing run time controlled activities 9 START REQUEST L Request to start injection cycle for example by start key on any module
22. as instrument or application problems Algae grow in aqueous media preferably in a pH range of 4 8 Their growth is accelerated by buffers for example phosphate or acetate Since algae grow through photosynthesis light will also stimulate their growth Even in distilled water small sized algae grow after some time Instrumental Problems Associated With Algae Algae deposit and grow everywhere within the HPLC system causing Blocked solvent filters or deposits on inlet or outlet valves resulting in unstable flow composition or gradient problems or a complete failure of the pump Small pore high pressure solvent filters usually placed before the injector to plug resulting in high system pressure PTFE frits blockage leading to increased system pressure Column filters to plug giving high system pressure Flow cell windows of detectors to become dirty resulting in higher noise levels since the detector is the last module in the flow path this problem is less common How to Prevent and or Reduce the Algae Problem Always use freshly prepared solvents especially use demineralized water which was filtered through about 0 2 um filters Never leave mobile phase in the instrument for several days without flow Always discard old mobile phase Use the amber solvent bottle Solvent bottle amber p n 9301 1450 supplied with the instrument for your aqueous mobile phase If possible add a few mg l sodium azide or a few percent organi
23. board Remote Detectors G1314B VWD VL 2 Yes No Yes 1 Yes G1314C VWD VL G1314E F VWD 2 No Yes Yes 1 Yes G4212A B DAD 2 No Yes Yes 1 Yes G1315C DAD VL 2 No Yes Yes 2 Yes G1365C MWD G1315D DAD VL G1365D MWD VL G1321B FLD 2 Yes No Yes 1 Yes G1362A RID G4280A ELSD No No No Yes Yes Yes EXT Contact AUTOZERO Others G1170A Valve Drive 2 No No No No No Requires a HOST module with on board LAN e g G4212A or G4220A with minimum firmware B 06 40 or C 06 40 or with additional G1369C LAN Card G1316A C TCC 2 No No Yes No Yes G1322A DEG No No No No No Yes AUX G1379B DEG No No No Yes No Yes G4225A DEG No No No Yes No Yes G4227A Flex Cube 2 No No No No No G4240A CHIP CUBE 2 Yes No Yes No Yes CAN DC OUT for CAN slaves THERMOSTAT for G1330A B NOT USED 198 1260 Binary Pump VL User Manual Hardware Information 12 The detector DAD MWD FLD VWD RID is the preferred access point for control via LAN The inter module communication is done via CAN e CAN connectors as interface to other modules e LAN connector as interface to the control software RS 232C as interface to a computer e REMOTE connector as interface to other Agilent products e Analog output connector s for signal output 1260 Binary Pump VL User Manual 199 12 Hardware Information 200 Overview Interfaces CAN The CAN is inter module communication interface It is a 2 wire serial bus system supporting high speed data communication and r
24. bottles into the solvent cabinet Connect the bottle heads of solvents A1 and A2 to the inlets in the upper row see labels on valve assembly Connect the bottle heads of solvents B1 and B2 to the inlets in the lower row see labels on valve assembly To solvent bottles Outlets For controlling the solvent selection valve please refer to the online help or user manual of your control software 1260 Binary Pump VL User Manual 155 9 Maintenance Exchanging the Solvent Selection Valve If leaking internally croossflow between the ports or if one of the channels is blocked When Tools required p n Description 8710 0899 Screwdriver Pozidriv 1 Parts required p n Description G1381 60000 Solvent Selection Valve Upgrade Kit Preparations Switch off the pump at the main power switch 1 Lift solvent bottles out of the solvent cabinet and place them on the table Disconnect the solvent tubes from the solvent selection valve and empty the tubes into the bottles Place the bottles back into the solvent cabinet 2 Disconnect all tubings from the solvent selection valve ra DA A f zng a PSN A Ng As 156 1260 Binary Pump VL User Manual Maintenance 9 Exchanging the Solvent Selection Valve 3 Using a Pozidriv screwdriver 1 loosen the holding screws of the valve holder 4 Carefully pull the valve holder out and disconnect the valve cable at the connecto
25. completed Table 20 Forced Cold Start Settings without on board LAN Mode Select sw sw2 sw3 SW4 SW5 SW6 sSW7 Swe TEST BOOT 1 1 0 0 1 0 0 1 1260 Binary Pump VL User Manual 207 12 Hardware Information Setting the 8 bit Configuration Switch without On board LAN 208 1260 Binary Pump VL User Manual 1260 Binary Pump VL User Manual 13 Appendix General Safety Information 210 The Waste Electrical and Electronic Equipment Directive 213 Batteries Information 214 Radio Interference 215 Sound Emission 216 Agilent Technologies on Internet 217 This chapter provides addition information on safety legal and web ee Agilent Technologies 209 13 Appendix General Safety Information General Safety Information The following general safety precautions must be observed during all phases of operation service and repair of this instrument Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design manufacture and intended use of the instrument Agilent Technologies assumes no liability for the customer s failure to comply with these requirements Ensure the proper usage of the equipment The protection provided by the equipment may be impaired gt The operator of this instrument is advised to use the equipment in a manner as specified in this manual Safety Standards This is a Safety Class I instrument provided with terminal for prote
26. corrode parts in the flow path Consider specifications for the pH range given for different materials like flow cells valve materials etc and recommendations in subsequent sections Solvent compatibility for stainless steel in standard LC systems Stainless steel is inert against many common solvents It is stable in the presence of acids and bases in the pH range specified for standard HPLC pH 1 12 5 It can be corroded by acids below pH 2 3 In general following solvents may cause corrosion and should be avoided with stainless steel Solutions of alkali halides their respective acids for example lithium iodide potassium chloride and so on and aequous solutions of halogenes High concentrations of inorganic acids like nitric acid sulfuric acid and organic solvents especially at higher temperatures replace if your chromatography method allows by phosphoric acid or phosphate buffer which are less corrosive against stainless steel Halogenated solvents or mixtures which form radicals and or acids for example 2 CHCl O 2 COCl 2 HCl This reaction in which stainless steel probably acts as a catalyst occurs quickly with dried chloroform if the drying process removes the stabilizing alcohol Chromatographic grade ethers which can contain peroxides for example THF dioxane di isopropylether such ethers should be filtered through dry aluminium oxide which adsorbs the peroxides 1260 Binary Pump VL User Ma
27. identical It plugs into the power input socket at the rear The male end of each power cord is different and designed to match the wall socket of a particular country or region Absence of ground connection or use of unspecified power cord The absence of ground connection or the use of unspecified power cord can lead to electric shock or short circuit gt Never operate your instrumentation from a power outlet that has no ground connection gt Never use a power cord other than the Agilent Technologies power cord designed for your region Use of unsupplied cables Using cables not supplied by Agilent Technologies can lead to damage of the electronic components or personal injury gt Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations 1260 Binary Pump VL User Manual 21 2 Site Requirements and Specifications WARNING Unintended use of supplied power cords Using power cords for unintended purposes can lead to personal injury or damage of electronic equipment gt Never use the power cords that Agilent Technologies supplies with this instrument for any other equipment Bench Space The module dimensions and weight see Table 2 on page 23 allow you to place the module on almost any desk or laboratory bench It needs an additional 2 5 cm 1 0 inches of space on either side and approximately 8 cm 8 1 inches in the
28. in the standard scope of delivery Items identified as not orderable can be downloaded from the Agilent website http www agilent com Either one of the three columns listed will be part of the delivery as ordered 1260 Binary Pump VL User Manual 29 3 30 Installing the Pump Accessory Kit Accessory Kit p n G1311 68755 p n 5062 2461 5063 6527 5181 1519 G1329 87300 G1312 87303 5042 9954 Description Waste tube 5 m reorder pack Tubing assembly i d 6 mm o d 9 mm 1 2 m to waste CAN cable Agilent module to module 1 m StS Capillary 0 17 mm 900 mm pump to thermostatted autosampler StS Capillary 0 17 mm 400 mm pump to injector Tubing clip 2x re order 4 pk 1260 Binary Pump VL User Manual Installing the Pump 3 Optimizing the Stack Configuration If your module is part of a complete Agilent 1260 Infinity Liquid Chromatograph you can ensure optimum performance by installing the following configurations These configurations optimize the system flow path ensuring minimum delay volume One Stack Configuration Ensure optimum performance by installing the modules of the Agilent 1260 Infinity LC System in the following configuration See Figure 4 on page 32 and Figure 5 on page 33 This configuration optimizes the flow path for minimum delay volume and minimizes the bench space required 1260 Binary Pump VL User Manual 31 3 Installing the Pump Optimizing the Stack Configuration
29. necessary to follow the procedure below to prevent clogging of the pump by salt precipitation or residual liquid droplets in parts of the system p n Description 1 Purging solvent s see Table 4 on page 52 1 5022 2184 Union ZDV Remove the column and replace it by a ZDV fitting Prepare bottles with appropriate intermediate solvents see Table 4 on page 52 1 Ifthe channel is not filled with buffer proceed to step 4 Place the solvent intake filter into a bottle of water Flush the channel at a flow rate suitable for the installed tubing typically 3 5 mL min for 10 min 4 Modify the flow path of your system as required for your application For delay volume optimization see the Rapid Resolution System manual Buffer salt of aqueous buffers may precipitate in residual isopropanol Capillaries and filter may be clogged by precipitating salt gt Flush solvent lines containing high concentration of salts first with water before introducing organic solvent gt Do not perform steps 5 to 7 for channels running with aqueous buffer as solvent 5 Replace the solvent bottle by a bottle of isopropanol 6 Flush the channel at a flow rate suitable for the installed tubing typically 3 5 mL min for 5 min 7 Swap the bottle of isopropanol with a bottle of solvent for your application 8 Repeat steps 1 to 7 for the other channel s of the pump 9 Install the desired column set the required composition and flow rate for you
30. packaging of electronics and mechanical assemblies This concept is based upon the use of expanded polypropylene EPP layers of foam plastic spacers in which the mechanical and electronic boards components of the module are placed This pack is then housed in a metal inner cabinet which is enclosed by a plastic external cabinet The advantages of this packaging technology are e virtual elimination of fixing screws bolts or ties reducing the number of components and increasing the speed of assembly disassembly e the plastic layers have air channels molded into them so that cooling air can be guided exactly to the required locations e the plastic layers help cushion the electronic and mechanical parts from physical shock and e the metal inner cabinet shields the internal electronics from electromagnetic interference and also helps to reduce or eliminate radio frequency emissions from the instrument itself 18 1260 Binary Pump VL User Manual 1260 Binary Pump VL User Manual ee 2 Site Requirements and Specifications e i e j Site Requirements 20 Physical Specifications 23 Performance Specifications 24 This chapter provides information on environmental requirements physical and performance specifications te Agilent Technologies 19 2 Site Requirements and Specifications Site Requirements A suitable environment is important to ensure optimal performance of the instrument Power Considerations
31. position before expected the motor current increases as the module attempts to drive the piston beyond the mechanical stop This current increase causes the error message to be generated Probable cause Suggested actions 1 Defective pump drive assembly Please contact your Agilent service representative Initialization Failed Error ID 2207 2217 Initialization Failed Pump channel A B Initialization Failed Pump channel B The module failed to initialize successfully within the maximum time window A maximum time is assigned for the complete pump initialization cycle If the time is exceeded before initialization is complete the error message is generated Probable cause Suggested actions 1 Blocked active inlet valve Exchange the active inlet valve 2 Defective pump drive assembly Please contact your Agilent service representative 3 Defective main board Please contact your Agilent service representative 1260 Binary Pump VL User Manual 107 7 108 Error Information Wait Timeout Error ID 2053 When running certain tests in the diagnostics mode or other special applications the pump must wait for the pistons to reach a specific position or must wait for a certain pressure or flow to be reached Each action or state must be completed within the timeout period otherwise the error message is generated Possible Reasons for a Wait Timeout Pressure not reached Pump channel A did not reach the delivery ph
32. rear for air circulation and electric connections If the bench shall carry a complete HPLC system make sure that the bench is designed to bear the weight of all modules The module should be operated in a horizontal position Condensation CAUTION Condensation within the module Condensation will damage the system electronics gt Do not store ship or use your module under conditions where temperature fluctuations could cause condensation within the module gt If your module was shipped in cold weather leave it in its box and allow it to warm slowly to room temperature to avoid condensation 22 1260 Binary Pump VL User Manual Physical Specifications Site Requirements and Specifications 2 Table2 Physical Specifications Type Specification Comments Weight 15 5 kg 34 Ibs Dimensions 180 x 345 x 435 mm height x width x depth Line voltage Line frequency Power consumption Ambient operating temperature Ambient non operating temperature Humidity Operating altitude Non operating altitude Safety standards IEC CSA UL 7 x 13 5 x 17 inches 100 240 VAC 10 50 or 60 Hz 5 220 VA 74 W 253 BTU 4 55 C 41 131 F 40 70 C 4 158 F lt 95 at 25 40 C 77 104 F Up to 2000 m 6562 ft Up to 4600 m 15091 ft Installation category Il Pollution degree 2 Wide ranging capability Maximum Non condensing For storing the module For in
33. the main power switch Remove the front cover to have access to the pump mechanics Remove the pump head see Removing the Pump Head Assembly on page 135 Both pump head assemblies use the same internal components In addition pump head A is fitted with the purge valve The following procedure describes the removal and disassembly of pump head A left For pump head B right proceed in the same way and skip steps that deal with the purge valve 1260 Binary Pump VL User Manual 137 9 Maintenance Maintenance of a P p Head without Seal Was 1 Place the pump head on a flat surface Loosen the lock screw two turns and while holding the lower half of the assembly piston housing carefully pull the pump housing away from the piston housing Lock screw Pump housing Piston housing 2 Remove the support rings from the piston housing and lift the housing away from the pistons Support ring hs Piston housing Piston 2 3 Check the piston surface and remove any deposits or layers Most suitable is polishing of the piston rod with toothpaste Replace the piston if scratched or if dents are visible Piston surface The best way to inspect a piston is to hold it up and watch e g a light bulb through the piston rod The transparent sapphire acts as a very strong magnifier and even smallest surface abnormalities become visible 138 1260 Binary Pump VL User Manual Maintenance 9 Ma
34. to control software RAN Bu Bus cable j to instant Pilot hermo cable optional 0 Remote cable CAN Bus cable AC Power Figure 7 Recommended Two Stack Configuration for 1260 Infinity Rear View 1260 Binary Pump VL User Manual 35 3 Installing the Pump Installing the Binary Pump Parts required Preparations p n Description 1 Pump 1 Data System and or 1 G4208A Instant Pilot 1 Power cord For other cables see text below and Cable Overview on page 178 Locate bench space Provide power connections Unpack the module Module is partially energized when switched off as long as the power cord is plugged in Repair work at the module can lead to personal injuries e g shock hazard when the cover is opened and the module is connected to power gt Make sure that it is always possible to access the power plug gt Remove the power cable from the instrument before opening the cover gt Do not connect the power cable to the Instrument while the covers are removed CAUTION Defective on arrival problems If there are signs of damage please do not attempt to install the module Inspection by Agilent is required to evaluate if the instrument is in good condition or damaged gt Notify your Agilent sales and service office about the damage gt An Agilent service representative will inspect the instrument at your site and initiate appropriate a
35. used in this product The apparatus is marked with this symbol when hot surfaces are available and the user should not touch it when heated up WARNING es alerts you to situations that could cause physical injury or death gt Do not proceed beyond a warning until you have fully understood and met the indicated conditions CAUTION A CAUTION alerts you to situations that could cause loss of data or damage of equipment gt Do not proceed beyond a caution until you have fully understood and met the indicated conditions 212 1260 Binary Pump VL User Manual Appendix 13 The Waste Electrical and Electronic Equipment Directive Abstract The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC adopted by EU Commission on 13 February 2003 is introducing producer responsibility on all electric and electronic appliances starting with 13 August 2005 This product complies with the WEEE Directive 2002 96 EC marking requirements The affixed label indicates that you must not discard this electrical electronic product in domestic household waste Product Category With reference to the equipment types in the WEEE Directive Annex I this product is classed as a Monitoring and Control Instrumentation product Do not dispose off in domestic household waste To return unwanted products contact your local Agilent office or see www agilent com for more information 1260 Binary Pump VL User Manual
36. valve in channel B 123 8 Test Functions and Calibration Leak Test 124 1260 Binary Pump VL User Manual 1260 Binary Pump VL User Manual 9 Maintenance Introduction to Maintenance and Repair 126 Warnings and Cautions 127 Overview of Maintenance 128 Cleaning the Module 131 Checking and Cleaning the Solvent Filter 132 Exchanging the Purge Valve Frit or the Purge Valve 133 Removing the Pump Head Assembly 135 Maintenance of a Pump Head without Seal Wash 137 Maintenance of a Pump Head with Seal Wash 141 Reinstalling the Pump Head Assembly 145 Seal Wear in Procedure 147 Exchanging the Active Inlet Valve or its Cartridge 148 Exchanging the Outlet Valve 152 Installation of the Solvent Selection Valve Upgrade Kit 154 Exchanging the Solvent Selection Valve 156 Exchanging the Optional Interface Board 159 Replacing the Module Firmware 160 This chapter describes the maintenance of the module ott Agilent Technologies 125 9 Maintenance Introduction to Maintenance and Repair The module is designed for easy repair The most frequent repairs such as piston seal change and purge valve frit change can be done from the front of the module with the module in place in the system stack These repairs are described in Simple Repairs on page 130 126 1260 Binary Pump VL User Manual Maintenance 9 Warnings and Cautions Toxic flammable and hazardous solvents samples and reagents The handling of solvents samples and rea
37. 1 White 1 White Digital ground 2 Brown 2 Brown Prepare run Low 3 Gray 3 Gray Start Low 4 Blue 4 Blue Shut down Low 5 Pink 5 Pink Not connected 6 Yellow 6 Yellow Power on High 7 Red 7 Red Ready High 8 Green 8 Green Stop Low 9 Black 9 Black Start request Low 1260 Binary Pump VL User Manual 183 11 184 Identifying Cables Agilent Module to General Purpose p n 01046 60201 Wire Color Pin Agilent Signal Name Active module TTL White 1 Digital ground A Q 1 ou Brown 2 Prepare run Low 00 pees DE KEY Gray 3 Start Low oo o0 te Blue 4 Shut down Low og Pink 5 Not connected oo 26 Yellow 6 Power on High 5 15 Red 7 Ready High Green 8 Stop Low Black 9 Start request Low 1260 Binary Pump VL User Manual BCD Cables Identifying Cables 11 One end of these cables provides a 15 pin BCD connector to be connected to the Agilent modules The other end depends on the instrument to be connected to Agilent Module to General Purpose p n G1351 81600 Wire Color Pin Agilent Signal Name BCD Digit module Green 1 BCD 5 20 Violet 2 BCD7 80 A i 3 BCD 6 40 2 ellow 4 BCD 4 10 Black 5 BCD 0 1 Ras Orange 6 BCD 3 8 Red 7 BCD 2 4 Brown 8 BCD 1 2 Gray 9 Digital ground Gray Gray pink 10 BCD 11 800 Red blue 11 BCD 10 400 White green 12 BCD 9 200 Brown green 13 BCD 8 100 notconnected 14
38. 10 Chloroform 100 Cyclohexane 118 Ethanol 114 Ethyl acetate 104 Heptane 120 Hexane 150 Isobutanol 100 lIsopropanol 100 Methanol 120 1 Propanol 100 Toluene 87 Water 46 6 Repeat step 1 on page 72 through step 5 on page 72 for the B channel of your binary pump 1260 Binary Pump VL User Manual 73 5 Optimizing Performance How to Optimize the Compressibility Compensation Setting 74 1260 Binary Pump VL User Manual 1260 Binary Pump VL User Manual 6 Troubleshooting and Diagnostics Overview of the Pump s Indicators and Test Functions 76 Status Indicators 78 Power Supply Indicator 78 Module Status Indicator 79 User Interfaces 80 Agilent Lab Advisor Software 81 Overview about the troubleshooting and diagnostic features ee Agilent Technologies 75 6 Troubleshooting and Diagnostics Overview of the Pump s Indicators and Test Functions Status Indicators The module is provided with two status indicators which indicate the operational state prerun run and error states of the module The status indicators provide a quick visual check of the operation of the module Error Messages In the event of an electronic mechanical or hydraulic failure the module generates an error message in the user interface For each message a short description of the failure a list of probable causes of the problem and a list of suggested actions to fix the problem are provided see chapter Error Information Test Functions
39. 160 The installation of newer firmware might be necessary if a newer version solves problems of older versions or to keep all systems on the same validated revision The installation of older firmware might be necessary to keep all systems on the same validated revision or ifa new module with newer firmware is added to a system or if third part control software requires a special version Description LAN RS 232 Firmware Update Tool Agilent Diagnostic Software Instant Pilot G4208A only if supported by module Description 1 Firmware tools and documentation from Agilent web site Read update documentation provided with the Firmware Update Tool To upgrade downgrade the module s firmware carry out the following steps 1 Download the required module firmware the latest LAN RS 232 FW Update Tool and the documentation from the Agilent web http www chem agilent com scripts cag_firmware asp 2 For loading the firmware into the module follow the instructions in the documentation Module Specific Information There is no specific information for this module 1260 Binary Pump VL User Manual 1260 Binary Pump VL User Manual 10 Parts for Maintenance Pump Head Assembly Without Seal Wash 162 Pump Head Assembly with Seal Wash 164 Outlet Valve 166 Purge Valve Assembly 167 Active Inlet Valve Assembly 168 Accessory Kit 169 Active Seal Wash Option 169 Solvent Cabinet 170 Bottle Head Assembly 171
40. 200 bar When used above 200 bar their lifetime will be significantly reduced 70 1260 Binary Pump VL User Manual Optimizing Performance 5 When to Remove the Static Mixer The binary pump is equipped with a static mixer The total delay volume of the pump is 600 900 ul The mixer has a volume of 420 ul The static mixer and both connecting capillaries can be replaced by a small capillary G1312 67301 under the following conditions e the delay volume of the pump should be reduced to a minimum for fastest gradient response and e the detector is used at medium or low sensitivity Removing the mixer will result in an increase of the composition ripple and higher detector noise 1260 Binary Pump VL User Manual 71 5 Optimizing Performance How to Optimize the Compressibility Compensation Setting The compressibility compensation default settings are 50 x 10 bar best for most aqueous solutions for pump head A and 115 x 106 bar to suit organic solvents for pump head B The settings represent average values for aqueous solvents A side and organic solvents B side Therefore it is always recommended to use the aqueous solvent on the A side of the pump and the organic solvent on the B side Under normal conditions the default settings reduce the pressure pulsation to values below 1 of system pressure that will be sufficient for most applications If the compressibility values for the solvents used differ from the def
41. 5 bench space 22 blockage 101 bottle head assembly 1 1 171 buffer application 54 buffer solutions 10 cable analog 180 BCD 185 CAN 187 connecting APG remote 35 connecting CAN 35 connecting LAN 35 connecting the ChemStation 35 connecting the power 35 external contact 188 interface 38 LAN 187 remote 182 RS 232 189 cables analog 1 8 BCD 178 CAN 179 external contact 179 LAN 179 overview 1 8 remote 1 8 RS 232 179 cable 187 changing solvents 51 cleaning 131 column 10 Communication settings RS 232C 205 communications 25 compensation sensor open 90 compensation sensor short 90 composition precision 25 25 composition range 25 compressibility compensation 15 24 72 condensation 22 configuration low flowrates 54 one stack 31 31 two stack 34 connections flow 42 45 control software 41 41 D damaged parts 29 damping unit 14 defect on arrival 28 delay volume 71 delay volume 15 24 design 11 Diagnostic software 81 dimensions 23 dual piston in series design 11 E electrical connections descriptions of 195 1260 Binary Pump VL User Manual electronic waste 213 electrostatic discharge ESD 159 EMF early maintenance feedback 17 encoder missing 102 error messages lost CAN partner 88 remote timeout 88 error messages AlV fuse 99 compensation sensor open 90 compensation sensor short 90 encoder missing 102 fan failed 91 ignition without cover 92 92 index a
42. 78 power consideration 20 power cords 21 pressure above upper limit 94 pressure below lower limit 95 pressure range 70 pressure sensor readings 40 pressure signal missing 96 pressure test 112 pressure pulsation 16 24 72 pressure test 77 pressure operating range 24 priming witha pump 50 68 with a syringe 68 PTFE frit 130 pump head missing 104 pump parameters auxiliary 59 pump seals alternative material 70 for normal phase solvents 70 urge valve 54 wW urge valve 130 ge purge valve assembly 14 radio interference 215 recommended pH range 24 remote cable 182 removing the pump head 135 repair procedures 130 repairs replacing firmware 160 160 restart without cover 93 results pressure test 115 RS 232C cable 189 communication settings 205 running the leak test 119 running the pressure test 114 S safety class 210 safety information lithium batteries 214 safety general information 210 standards 23 symbols 212 sapphire piston 13 sealwash 11 seal wear counter 140 seal wash wash solvent 69 when touse 69 seals alternative material 70 normal phase 70 security lever 37 selection valve fuse 98 servo restart failed 103 1260 Binary Pump VL User Manual setable flowrange 24 shutdown 87 sieve 130 simple repair procedures 130 simple repairs 126 site requirements 19 power cords 21 snap fastener 43 46 solubility gas 54 solvent cabinet 43 46 solvent filters checking 132 cleaning 132 preven
43. Grey pink 11 Not connected Red blue 12 Not connected White green 13 Not connected Brown green 14 Not connected White yellow 15 Not connected 1260 Binary Pump VL User Manual Identifying Cables 11 Agilent Module to PC p n Description G1530 60600 RS 232 cable 2m RS232 61600 RS 232 cable 2 5m Instrument to PC 9 to 9 pin female This cable has special pin out and is not compatible with connecting printers and plotters It s also called Null Modem Cable with full handshaking where the wiring is made between pins 1 1 2 3 3 2 4 6 5 5 6 4 7 8 8 7 9 9 5181 1561 RS 232 cable 8m 1260 Binary Pump VL User Manual 189 11 Identifying Cables Agilent 1200 Module to Printer p n Description 5181 1529 Cable Printer Serial amp Parallel is a SUB D 9 pin female vs Centronics connector on the other end NOT FOR FW UPDATE For use with G1323 Control Module 190 1260 Binary Pump VL User Manual 1260 Binary Pump VL User Manual 12 Hardware Information Firmware Description 192 Electrical Connections 195 Rear View of the Module 196 Interfaces 197 Overview Interfaces 200 Setting the 8 bit Configuration Switch without On board LAN 204 Communication Settings for RS 232C 205 Special Settings 207 This chapter describes the pump in more detail on hardware and electronics ee Agilent Technologies 191 12 Hardware Information Firmware Description The firmware of the instrument consists of two independent secti
44. Hydraulic Path with Solvent Selection Valve 172 Hydraulic Path without Solvent Selection Valve 174 This chapter provides information on parts for maintenance ee Agilent Technologies 161 10 Parts for Maintenance Pump Head Assembly Without Seal Wash Item p n Description 1 5063 6586 Sapphire piston 2 G1311 60002 Piston housing 3 5067 1560 Support Ring SL no seal wash 4 5062 2484 Gasket seal wash pack of 6 5 5042 8952 Seal holder 6 G1312 67300 Capillary outlet valve to piston 2 7 5063 6589 Piston seal PTFE carbon filled black pack of 2 default 8 G1311 25200 Pump chamber housing 9 0515 0175 Mounting screw for manual purge valve holder M4 20 mm long 10 G1312 23200 Holder for manual purge valve 11 G1312 60061 Purge valve 1260 12 G1312 60067 Outlet valve 1220 1260 13 5042 1303 Lock screw 14 G1312 60025 Active inlet valve body without cartridge G1312 60020 Cartridge for active inlet valve 600 bar 15 G1312 23201 Adapter 16 0515 2118 Pump head screw M5 60 mm 162 1260 Binary Pump VL User Manual Parts for Maintenance Pump Head Assembly Without Seal Wash Figure 17 Pump Head Assembly Without Seal Wash 1260 Binary Pump VL User Manual 10 163 10 Parts for Maintenance Pump Head Assembly with Seal Wash Item p n Description 1 5065 9953 Seal wash pump assembly 5042 8507 Peristaltic pump cartridge silicone tubing 2 5063 6586 Sapphire piston 3 G1311 60002 Piston housing 4 01018 60027 Support ring sea
45. L This is the capacity maximum possible volume in liter of the solvent bottle In default 0 L combination with the actual volume this parameter is used for calculating and displaying the relative liquid level Actual 0 1000L After filling the solvent bottles enter the actual volumes into these boxes The Volume default 0 L Actual Volume must not be larger than the Total Volume of the bottle Prevent default unchecked If this option is checked the pump won t start a new run if the solvent level in analysis one or more bottles is below the minimum volume Enter a minimum volume in Turn pump off default unchecked liter which considers the position of the solvent inlet and size shape of the solvent bottle such that no air is drawn if the actual volume gets close to this limit If this option is checked the pump will turn off before air is aspirated However the residual solvent volume has been calculated for 1 L solvent bottles and may be too small for large bottles or other vessels 1260 Binary Pump VL User Manual 61 4 Using the Binary Pump Solvent Information 62 Observe the following recommendations on the use of solvents Follow recommendations for avoiding the growth of algae see Algae Growth in HPLC Systems on page 65 Small particles can permanently block capillaries and valves Therefore always filter solvents through 0 4 um filters Avoid or minimize the use of solvents that may
46. LC grade water 90 and isopropanol 10 as wash solvent This mixture prevents bacteria growth in the wash bottle and reduces the surface tension of the water In order to avoid accumulation of buffer salts or impurities regularly replace the washing solution using fresh solvents The operation of the peristaltic pump can be controlled from the data system or the Instant Pilot For adding a seal wash option please contact your local Agilent Technologies service representative 1260 Binary Pump VL User Manual 69 5 Optimizing Performance When to Use Alternative Seals The standard seals for the binary pump can be used for most applications However normal phase applications for example hexane are not compatible with the standard seals They cause extremely high abrasion and significantly shorten seal life time For the use with normal phase applications special polyethylene pistons seals yellow color PE seals pack of 2 p n 0905 1420 are available These seals have less abrasion compared to the standard seals WARNING The seal wear in procedure causes problems to the normal phase seals yellow They will be destroyed by the procedure gt DO NOT apply the seal wear in procedure performed to normal phase seals 1 Remove the standard seals from the pump head Maintenance of a Pump Head without Seal Wash on page 137 2 Install normal phase seals Polyethylene seals have a limited pressure range of 0
47. Rear of the module is exposed to strong direct sunlight Suggested actions Please contact your Agilent service representative Please contact your Agilent service representative Please contact your Agilent service representative Ensure that the rear of module is not directly exposed to strong sunlight 1260 Binary Pump VL User Manual Error Information 7 Module Error Messages Restart Without Cover Error ID 2502 The module was restarted with the top cover and foam open The sensor on the main board detects when the top foam is in place If the module is restarted with the foam removed the module switches off within 30 s and the error message is generated Probable cause Suggested actions 1 The module started with the top cover and Please contact your Agilent service foam removed representative 2 Rear of the module is exposed to strong Ensure that the rear of module is not directly direct sunlight exposed to strong sunlight Solvent Zero Counter Error ID 2055 2524 Pump firmware version A 02 32 and higher allow to set solvent bottle fillings in the data system If the volume level in the bottle falls below the specified value the error message appears when the feature is configured accordingly Probable cause Suggested actions 1 Volume in bottle below specified volume Refill bottles and reset solvent counters 2 Incorrect setting Make sure the limits are set correctly 1260 Binary Pump VL User Manual 93
48. Receiver is the autosampler Special Interfaces Some modules have module specific interfaces connectors They are described in the module documentation 1260 Binary Pump VL User Manual 203 12 Hardware Information Setting the 8 bit Configuration Switch without On board LAN 204 The 8 bit configuration switch is located at the rear of the module This module does not have its own on board LAN interface It can be controlled through the LAN interface of another module and a CAN connection to that module Figure 29 Configuration switch settings depend on configured mode All modules without on board LAN e default should be ALL DIPS DOWN best settings Bootp mode for LAN and 19200 baud 8 data bit 1 stop bit with no parity for RS 232 e DIP 1 DOWN and DIP 2 UP allows special RS 232 settings e for boot test modes DIPS 1 2 must be UP plus required mode For normal operation use the default best settings Switch settings provide configuration parameters for serial communication protocol and instrument specific initialization procedures With the introduction of the Agilent 1260 Infinity all GPIB interfaces have been removed The preferred communication is LAN 1260 Binary Pump VL User Manual Hardware Information 12 The following tables represent the configuration switch settings for the modules without on board LAN only Table 14 8 bit Configuration Switch without on board LAN Mode
49. a syringe is recommended when e vacuum degasser is used for the first time or vacuum tubes are empty or e changing to solvents that are immiscible with the solvent currently in the vacuum tubes Priming the vacuum degasser by using the pump at high flow rate 3 5 mL min is recommended when pump was turned off for a length of time for example during night and volatile solvent mixtures are used or solvents have been changed For more information see the Agilent 1260 Infinity Standard Degasser User Manual p n G1322 90012 1260 Binary Pump VL User Manual Optimizing Performance 5 When to Use the Active Seal Wash Option Concentrated buffer solutions will reduce the lifetime of the seals and pistons in your binary pump The active seal wash option allows to maintain the seal lifetime by flushing the low pressure side of the seals with a wash solvent The seal wash option is strongly recommended if buffer concentrations of 0 1 M or higher are used regularly with the pump The active seal wash option kit can be ordered by quoting Active Seal Wash Option kit p n G1312 68721 The seal wash option comprises a peristaltic pump secondary seals gaskets seal holders and tubing for both pump heads A bottle of premixed water isopropanol 90 10 vol is placed in the solvent cabinet and connected to the peristaltic pump as described in the technical note that comes with the active seal wash kit Always use a mixture of HP
50. ached If the time to reach the index position is too long the error message is generated Probable cause Suggested actions 1 Irregular or sticking drive movement Remove the pump head and examine the seals pistons and internal components for signs of wear contamination or damage Exchange components as required 2 Defective pump drive assembly Please contact your Agilent service representative Index Missing Error ID 2205 2215 2505 Index Missing Pump channel A B Index Missing Pump channel B The encoder index position in the module was not found during initialization During initialization the first piston is moved to the mechanical stop After reaching the mechanical stop the piston reverses direction until the encoder index position is reached If the index position is not recognized within a defined time the error message is generated Probable cause Suggested actions 1 Disconnected or defective encoder cable Please contact your Agilent service representative 2 Defective pump drive assembly Please contact your Agilent service representative 1260 Binary Pump VL User Manual Error Information 7 Stroke Length Error ID 2206 2216 Stroke Length Pump channel A B Stroke Length Pump channel B The distance between the lower piston position and the upper mechanical stop is out of limits pump During initialization the module monitors the drive current If the piston reaches the upper mechanical stop
51. age 160 If this does not help a main board replacement is required 1260 Binary Pump VL User Manual 79 6 Troubleshooting and Diagnostics User Interfaces 80 Depending on the user interface the available tests vary Some descriptions are only available in the Service Manual Table9 Test Functions available vs User Interface Test Instant Pilot G4208A Agilent Lab Advisor Pressure Test Yes Yes Leak Test Yes Yes 1260 Binary Pump VL User Manual Troubleshooting and Diagnostics 6 Agilent Lab Advisor Software The Agilent Lab Advisor software is a standalone product that can be used with or without data system Agilent Lab Advisor software helps to manage the lab for high quality chromatographic results and can monitor in real time a single Agilent LC or all the Agilent GCs and LCs configured on the lab intranet Agilent Lab Advisor software provides diagnostic capabilities for all Agilent 1200 Infinity Series modules This includes diagnostic capabilities calibration procedures and maintenance routines for all the maintenance routines The Agilent Lab Advisor software also allows users to monitor the status of their LC instruments The Early Maintenance Feedback EMF feature helps to carry out preventive maintenance In addition users can generate a status report for each individual LC instrument The tests and diagnostic features as provided by the Agilent Lab Advisor software may differ from the descriptions in th
52. al service or adjustment unless another person capable of rendering first aid and resuscitation is present Do not replace components with power cable connected Do not operate the instrument in the presence of flammable gases or fumes Operation of any electrical instrument in such an environment constitutes a definite safety hazard Do not install substitute parts or make any unauthorized modification to the instrument Capacitors inside the instrument may still be charged even though the instrument has been disconnected from its source of supply Dangerous voltages capable of causing serious personal injury are present in this instrument Use extreme caution when handling testing and adjusting When working with solvents observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet by the solvent vendor especially when toxic or hazardous solvents are used 1260 Binary Pump VL User Manual 211 13 Appendix Safety Symbols Table 21 Safety Symbols Symbol Description The apparatus is marked with this symbol when the user should refer to the A instruction manual in order to protect risk of harm to the operator and to protect the apparatus against damage Indicates dangerous voltages Indicates a protected ground terminal Indicates eye damage may result from directly viewing the light produced G by the deuterium lamp
53. al wash option 1260 Binary Pump VL User Manual 173 10 Parts for Maintenance Hydraulic Path without Solvent Selection Valve Item p n 1 2 3 OR 10 174 G1311 60003 G1312 67307 61312 87330 G1312 67308 G1312 67300 G1312 67304 G1312 67302 2x 0100 1847 5062 2461 61312 67305 G1329 87300 G1312 67301 Description Bottle head assembly Capillary damper to mixer Mixer Capillary mixer to purge valve Capillary outlet valve to piston 2 Capillary mixing chamber to damper inlet Capillary channel A and B pump head outlet to mixing chamber included PEEK adapter 1 4 28 to 10 32 Adapter AIV to solvent inlet tubes Waste tube 5 m reorder pack Outlet capillary pump to injector Capillary pump to thermostattable autosampler Capillary damper to purge valve Capillary needed when pump used without mixer installed 1260 Binary Pump VL User Manual Parts for Maintenance 10 Hydraulic Path without Solvent Selection Valve Ph D 2G FO 7 AS lt Seas e aa Figure 25 Hydraulic Path without Solvent Selection Valve 1260 Binary Pump VL User Manual 175 10 Parts for Maintenance Hydraulic Path without Solvent Selection Valve 176 1260 Binary Pump VL User Manual 1260 Binary Pump VL User Manual 11 Identifying Cables 178 180 Remote Cables 182 BCD Cables 185 CAN Cable 187 External Contact Cable 188 Agilent Module to PC 189 Agilent 1200 Module to Printer Cable Overview
54. anual Suggested actions Please contact your Agilent service representative Please contact your Agilent service representative Please contact your Agilent service representative Please contact your Agilent service representative 91 7 92 Error Information Leak Error ID 0064 A leak was detected in the module The signals from the two temperature sensors leak sensor and board mounted temperature compensation sensor are used by the leak algorithm to determine whether a leak is present When a leak occurs the leak sensor is cooled by the solvent This changes the resistance of the leak sensor which is sensed by the leak sensor circuit on the main board Probable cause 1 2 3 Loose fittings Broken capillary Loose or leaking purge valve inlet valve or outlet valve Defective pump seals Open Cover Error ID 0205 The top foam has been removed Suggested actions Ensure all fittings are tight Exchange defective capillaries Ensure pump components are seated correctly If there are still signs of a leak exchange the appropriate seal purge valve inlet valve outlet valve Exchange the pump seals The sensor on the main board detects when the top foam is in place If the foam is removed the fan is switched off and the error message is generated Probable cause 1 The top foam was removed during operation Foam not activating the sensor Dirty or defective sensor
55. any available CAN port of the 1260 system 5 Plug a CAN cable into the CAN connector of the Instant Pilot The Standard Degasser must not be connected to LAN or CAN as its connector is for diagnostic use only 6 Connect the CAN cable to the CAN connector of one of the modules 7 Press in the power switches to turn on the modules The Agilent control software can also be connected to the system through a LAN cable which requires the installation of a LAN board For more information about connecting the Instant Pilot or Agilent control software refer to the respective user manual Interfaces on page 197 provides information on how to connect external hardware 1260 Binary Pump VL User Manual 41 3 Installing the Pump Flow Connections of the Binary Pump with Optional Solvent Selection Valve Parts required p n Description 1 Other modules 1 G1311 68755 Accessory Kit 2 wrenches 1 4 5 16 inch for capillary connections Preparations Pump is installed in the LC system WARNING When opening capillary or tube fittings solvents may leak out The handling of toxic and hazardous solvents and reagents can carry health risks gt Observe appropriate safety procedures for example wear goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used 42 1260 Binary Pump VL User Manual Instal
56. arameters for the two pump channels The active inlet valve is opened and the down moving piston draws solvent into the first pump head At the same time the second piston is moving upwards delivering into the system After a controller defined stroke length depending on the flow rate the drive motors are stopped and the active inlet valve is closed The motor direction is reversed and moves the first piston up until it reaches the stored upper limit and at the same time moving the second piston downwards Then the sequence starts again moving the pistons up and down between the two limits During the upward movement of the first piston the solvent in the pump head is pressed through the outlet ball valve into the second pumping unit The second piston draws in half of the volume displaced by the first piston and the remaining half volume is directly delivered into the system During the drawing stroke of the first piston the second piston delivers the drawn volume into the system 1260 Binary Pump VL User Manual Introduction 1 Table 1 Pump Details Delay volume From mixing point to pump outlet dependent on back pressure 180 480 uL without mixer 600 900 uL with mixer Materials in contact with mobile phase Pump head SST gold sapphire ceramic Active inlet valve SST sapphire ruby ceramic PTFE Outlet ball valve SST gold sapphire ruby tantalum Adapter SST gold Purge valve SST gold PTFE ceramic Damping unit
57. arge leaks visible at the pump seals Exchange the pump seals 6 Large leaks visible at active inlet valve Ensure the leaky components are installed outlet valve or purge valve tightly Exchange the component if required 120 1260 Binary Pump VL User Manual Test Functions and Calibration 8 Pressure limit not reached but plateaus horizontal or positive Probable cause 1 Degasser and pump channels A and or B not flushed sufficiently air in the channels 2 Wrong solvent All plateaus negative Probable cause 1 Loose or leaky fittings 2 Loose purge valve 3 Leaky mixer if installed 4 Contaminated purge valve 5 Loose pump head screws in channel A or B 6 Leaking seal or scratched piston in channel A2 or B2 7 Leaking outlet valve in channel A or B 8 Leaky damper 1260 Binary Pump VL User Manual Suggested actions Purge the degasser and pump channels thoroughly with isopropanol under pressure use the restriction capillary Install isopropanol Purge the degasser and pump channels thoroughly Suggested actions Ensure all fittings are tight or exchange capillary Tighten the purge valve 14 mm wrench Tighten the mixer fittings and nuts Open and close purge valve to flush out contamination Exchange the valve if still leaky Ensure the pump head screws in channels A and B are tight Exchange the pump seals in both channels Check the pistons for scratches Exchange if scratched
58. ase Pump channel B did not reach the delivery phase Pump channel A did not reach the take in phase Pump channel B did not reach the take in phase Solvent volume not delivered within the specified time Probable cause Suggested actions 1 Purge valve open Ensure that purge valve is closed 2 Leak at fittings purge valve active inlet Ensure pump components are seated valve outlet valve or piston seals correctly If there are still signs of a leak exchange the appropriate seal purge valve active inlet valve outlet valve piston seal Exchange defective capillaries 3 Flow changed after starting test Ensure correct operating condition for the special application in use 4 Defective pump drive assembly Please contact your Agilent service representative 1260 Binary Pump VL User Manual Error Information 7 Electronic fuse of SSV Error ID 2049 The electronic fuse protecting the solvent selection valve electronics has blown Probable cause Suggested actions 1 Recoverable error of the SSV electronic Restart module the electronic fuse can recover If not contact Agilent service 2 Short cut of SSV cable Replace cable between board and SSV 1260 Binary Pump VL User Manual 109 7 Error Information Module Error Messages 110 1260 Binary Pump VL User Manual 1260 Binary Pump VL User Manual 7 e Test Functions and Calibration e E e j Pressure Test 112 Description 112 Positionin
59. at the outlet of the previous module in the stack eg autosampler port 6 of the injection valve and repeat the pressure test Exclude each module one by one to determine which module is leaking Ifthe pump is determined to be the source of the leak run the leak test 1260 Binary Pump VL User Manual 115 8 Test Functions and Calibration Potential Causes of Pressure Test Failure System Pressure Test failed The test will fail if the sum of all leaks in the system pump autosampler or column compartment and connections exceeds the test limit After isolating and fixing the cause of the leak repeat the System Pressure Test to confirm the system is pressure tight Probable cause Suggested actions 1 Purge valve open Close the purge valve 2 Loose or leaky fittings Tighten the fitting or exchange the capillary 3 Pump Damaged pump seals or pistons Run the Leak Rate Test to confirm the leak 4 Loose purge valve Tighten the purge valve nut 14 mm wrench 5 Autosampler Loose or leaky fitting Tighten or exchange the fitting or capillary 6 Autosampler Rotor seal injection valve Exchange the rotor seal 7 Autosampler Damaged metering seal or Exchange the metering seal Check the piston piston for scratches Exchange the piston if required 8 Autosampler Needle seat Exchange the needle seat 9 Column compartment Loose or leaky fitting Tighten or exchange the fitting or capillary 10 Column compartment Rotor sea
60. at the solvent inlet tube connection points towards the front 150 1260 Binary Pump VL User Manual Maintenance 9 Exchanging the Active Inlet Valve or its Cartridge 9 Tighten the nut by turning the valve in its final position using a torque wrench 8 Nm J 10 Reconnect the Active Inlet Valve cable to the connector at the Z panel and the inlet tube to the valve 11 Reinstall the front cover NOTE After an exchange of the valve it may be required to pump several mL of the solvent used in the current application before the flow stabilizes at a pressure ripple as low as it used to be when the system was still working properly 1260 Binary Pump VL User Manual 151 9 Maintenance Exchanging the Outlet Valve When if leaking internally Tools required Description Wrench 1 4 1 5 inch Wrench 1 4 inch Wrench 14mm Parts required p n Description G1312 60067 Outlet valve 1220 1260 Preparations Switch off the pump at the main power switch 1 Using a inch wrench disconnect the absorber capillary from the outlet valve 2 Unscrew the valve with the 14 mm wrench and remove it from the pump body 3 Do not disassemble the outlet valve as this can damage the valve 4 Reinstall the outlet valve and tighten it using a torque wrench 12 Nm 152 1260 Binary Pump VL User Manual 5 Reconnect the capillary at the outlet valve Outlet valve capillary Outlet valve 1260 Binary Pump VL User Manual Maintenanc
61. ations 148 If internally leaking backflow Description Wrench 14mm p n Description G1312 60025 Active inlet valve body without cartridge G1312 60020 Cartridge for active inlet valve 600 bar Switch off pump at the main power switch and unplug the power cable Use an optional solvent shutoff valve or lift up solvent filters in solvent reservoirs for avoiding leakages 1 Remove the front cover 2 Unplug the active inlet valve cable from the connector Disconnect the solvent inlet tube at the inlet valve beware of leaking solvents Binary pumps without solvent selection valve SSV have an adapter installed between the solvent line and the active inlet valve AIV Disconnect the solvent tubes at the adapter and remove the adapter from the AIV 1260 Binary Pump VL User Manual Maintenance 9 Exchanging the Active Inlet Valve or its Cartridge 4 Using a 14 mm wrench loosen the active inlet valve and remove the valve from pump head Active inlet valve cable Active inlet valve 5 Using a pair of tweezers remove the valve cartridge from the defective active inlet valve 1260 Binary Pump VL User Manual 149 9 Maintenance Exchanging the Active Inlet Valve or its Cartridge 6 Push the cartridge into the new active inlet valve Active inlet valve cartridge Active inlet valve 7 Insert the valve into the pump head Using the 14 mm wrench turn the nut until it is hand tight 8 Position the valve such th
62. ations Switch off the pump at the main power switch CAUTION Damage of the pump drive Starting the pump when the pump head is removed may damage the pump drive gt Never start the pump when the pump head is removed Both pump head assemblies use the same internal components In addition pump head A is fitted with the purge valve The following procedure describes the removal and disassembly of pump head A left For pump head B right proceed in the same way and skip steps that deal with the purge valve 1260 Binary Pump VL User Manual 135 9 Maintenance Removing the Pump Head Assembly 1 Remove the front cover 2 Disconnect the capillaries at the back of the purge valve holder the pump head adapter and the tube at the active inlet valve Beware of leaking solvents 3 Using a 4 mm hexagonal key stepwise loosen and remove the two pump head screws Pump head screws 136 1260 Binary Pump VL User Manual Maintenance 9 Maintenance of a Pump Head without Seal Wash When Tools required Parts required OR Preparations In case of maintenance or pump head internal leaks p n Description 8710 0510 Wrench open 1 4 5 16 inch 8710 2411 Hex key 3 mm12 cm long 8710 2392 Hex key 4 mm15 cm long T handle 01018 23702 Insert tool p n Description 1 5063 6589 Piston seal PTFE carbon filled black pack of 2 default 1 0905 1420 PE seals pack of 2 1 5063 6586 Sapphire piston Switch off the pump at
63. ault settings it is recommended to change the compressibility values accordingly Compressibility settings can be optimized by using the values for various solvents described in Table 8 on page 73 If the solvent in use is not listed in the compressibility table when using premixed solvents and if the default settings are not sufficient for your application the following procedure can be used to optimize the compressibility settings 1 Start channel A of the binary pump with the required flow rate 2 Before starting the optimization procedure the flow must be stable Use degassed solvent only Check the tightness of the system with the pressure test see Description on page 112 3 Your pump must be connected to a data system or Instant Pilot with which the pressure and ripple can be monitored or connect an external measurement device to the analog pressure output see Electrical Connections on page 195 4 Start the recording device with the plot mode 5 Starting with a compressibility setting of 10 x 10 bar increase the value in steps of 10 Re zero the integrator as required The compressibility compensation setting that generates the smallest pressure ripple is the optimum value for your solvent composition 72 1260 Binary Pump VL User Manual Optimizing Performance 5 Table8 Solvent Compressibility Solvent pure Compressibility 10 bar Acetone 126 Acetonitrile 115 Benzene 95 Carbon tetrachloride 1
64. bar Each channel comprises a pump assembly including pump drive pump head active inlet valve which has a replaceable cartridge and outlet valve Both channels are connected in a low volume mixing chamber which is connected by a capillary coil to a damping unit and a mixer A purge valve including a PTFE frit is fitted at the pump outlet for convenient priming of the pumping system A seal wash optional is available when the pump is used with buffer solutions 1260 Binary Pump VL User Manual 11 1 Introduction Overview of the Binary Pump Purge valve Pump outlet To waste Outlet valve Outlet valve Inlet Val Inlet valve From solvent bottle From solvent bottle Seal Piston Pump head A Pump head B Figure2 The Hydraulic Path 12 1260 Binary Pump VL User Manual Introduction 1 Overview of the Binary Pump How Does the Binary Pump Work The liquid runs from the solvent reservoir through an active inlet valve Each side of the binary pump comprises two substantially identical pump units Both pump units comprise a ball screw drive and a pump head with two sapphire pistons for reciprocating movement To mixing chamber From solvent bottle r Piston Ball screw drive Motor with encoder Figure 3 Principle of Pump Unit 1260 Binary Pump VL User Manual 13 1 14 Introduction A servo controlled variable reluctance motor drives the two ball sc
65. c solvent to the aqueous mobile phase 1260 Binary Pump VL User Manual 65 4 Using the Binary Pump Algae Growth in HPLC Systems 66 1260 Binary Pump VL User Manual 1260 Binary Pump VL User Manual 5 Optimizing Performance When to Use a Vacuum Degasser 68 When to Use the Active Seal Wash Option 69 When to Use Alternative Seals 70 When to Remove the Static Mixer 71 How to Optimize the Compressibility Compensation Setting 72 This chapter gives hints on how to optimize the performance or use additional devices te Agilent Technologies 67 5 Optimizing Performance When to Use a Vacuum Degasser 68 The pump does not necessarily require degassing But for the following conditions the vacuum degasser is recommended e if your detector is used with maximum sensitivity in the low UV wavelength range e if your application requires highest injection precision or e if your application requires highest retention time reproducibility mandatory at flow rates below 0 5 mL min Operational Hints for the Vacuum Degasser If you are using the vacuum degasser for the first time if the vacuum degasser was switched off for any length of time for example overnight or if the vacuum degasser lines are empty you should prime the vacuum degasser before running an analysis The vacuum degasser can be primed either by drawing solvent through the degasser with a syringe or by pumping with the pump Priming the degasser with
66. ce it into your waste system 6 Ifthe binary pump is not part of an Agilent 1260 Infinity system stack or placed on the bottom of a stack connect the corrugated waste tube to the waste outlet of the pump leak handling system 7 Connect the outlet capillary binary pump to injection device to the outlet of the purge valve 46 1260 Binary Pump VL User Manual Installing the Pump 3 Flow Connections of the Binary Pump Without Solvent Selection Valve 8 Purge your system before first use see Initial Priming on page 48 a TIN Bottle head assembly ETS IEA Solevent cabinet Degasser Static mixer Purge valve Pump head channel B Pump head channel A Adapter Active inlet valve A Active inlet valve B Waste tubing Outlet capillary to autosampler Waste outlet Figure 13 Flow Connection of Binary Pump Without Solvent Selection Valve 1260 Binary Pump VL User Manual 47 3 Installing the Pump Priming the System Initial Priming When Parts required Preparations Before a degasser or solvent tubing can be used it is necessary to prime the system Isopropanol is recommended as priming solvent due to its miscibility with nearly all HPLC solvents and its excellent wetting properties Description 1 lsopropanol Connect all modules hydraulically as described in the respective module manuals Fill each solvent bottle with 100 mL isopropanol Switch the system on When ope
67. coder Missing Pump channel B The optical encoder on the pump motor in the module is missing or defective The processor checks the presence of the pump encoder connector every 2 s If the connector is not detected by the processor the error message is generated Probable cause Suggested actions 1 Defective or disconnected pump Please contact your Agilent service representative encoder connector 2 Defective pump drive assembly Please contact your Agilent service representative Inlet Valve Missing Error ID 2048 2052 Inlet Valve Missing Pump channel A B Inlet Valve Missing Pump channel B The active inlet valve in the module is missing or defective The processor checks the presence of the active inlet valve connector every 2 s If the connector is not detected by the processor the error message is generated Probable cause Suggested actions 1 Disconnected or defective cable Ensure the pins of the active inlet valve connector are not damaged Ensure the connector is seated securely 2 Disconnected or defective connection Please contact your Agilent service representative cable front panel to main board 3 Defective active inlet valve Exchange the active inlet valve 1260 Binary Pump VL User Manual Servo Restart Failed Error ID 2201 2211 Servo Restart Failed Pump channel A Error Information 7 B Servo Restart Failed Pump channel B The pump motor in the module was unable to move into the correct po
68. correctly screws Install the pump head correctly Ensure nothing not secured or pump head not seated e g capillary is trapped between the pump correctly head and body 2 Broken piston Exchange the piston 104 1260 Binary Pump VL User Manual Error Information 7 Index Limit Error ID 2203 2213 Index Limit Pump channel A B Index Limit Pump channel B The time required by the piston to reach the encoder index position was too short pump During initialization the first piston is moved to the mechanical stop After reaching the mechanical stop the piston reverses direction until the encoder index position is reached If the index position is reached too fast the error message is generated Probable cause Suggested actions 1 Irregular or sticking drive movement Remove the pump head and examine the seals pistons and internal components for signs of wear contamination or damage Exchange components as required 2 Defective pump drive assembly Please contact your Agilent service representative 1260 Binary Pump VL User Manual 105 7 106 Error Information Index Adjustment Error ID 2204 2214 Index Adjustment Pump channel A B Index Adjustment Pump channel B The encoder index position in the module is out of adjustment During initialization the first piston is moved to the mechanical stop After reaching the mechanical stop the piston reverses direction until the encoder index position is re
69. ctions 36 1260 Binary Pump VL User Manual Installing the Pump 3 Installing the Binary Pump 1 Place the module on the bench in a horizontal position 2 Ensure the power switches on the front of the modules are OFF switches stand out Status Indicator wt st Agta acing K eter Power switch Serial number Figure 8 Front of Binary Pump 3 At the rear of the binary pump move the security lever to its maximum right position 4 Connect the power cable to the power connector at the rear of the module The security lever will prevent that the cover is opened while the power cord is connected to the module 1260 Binary Pump VL User Manual 37 3 Installing the Pump Installing the Binary Pump 5 Connect the required interface cables to the rear of the binary pump see Connecting Modules and Control Software on page 39 Slot for interface board Remote Analog output CAN Bus Configuration Switch JE Asians Technologies Product Ne 013120 gt gt TH gt gt lt Seisi Ne DESonct10 Power plug Figure 9 Rear of the Binary Pump 6 Connect the capillary solvent tubes and waste tubings see Flow Connections of the Binary Pump with Optional Solvent Selection Valve on page 42 or Flow Connections of the Binary Pump W
70. ctive earthing and has been manufactured and tested according to international safety standards Operation 210 Before applying power comply with the installation section Additionally the following must be observed Do not remove instrument covers when operating Before the instrument is switched on all protective earth terminals extension cords auto transformers and devices connected to it must be connected to a protective earth via a ground socket Any interruption of the protective earth grounding will cause a potential shock hazard that could result in serious personal injury Whenever it is likely that the protection has been impaired 1260 Binary Pump VL User Manual Appendix 13 the instrument must be made inoperative and be secured against any intended operation Make sure that only fuses with the required rated current and of the specified type normal blow time delay and so on are used for replacement The use of repaired fuses and the short circuiting of fuse holders must be avoided Some adjustments described in the manual are made with power supplied to the instrument and protective covers removed Energy available at many points may if contacted result in personal injury Any adjustment maintenance and repair of the opened instrument under voltage should be avoided whenever possible When inevitable this has to be carried out by a skilled person who is aware of the hazard involved Do not attempt intern
71. djustment 106 index missing 106 index limit 105 initialization failed 107 inlet valve missing 102 eak sensor open 89 eak sensor short 89 eak 92 missing pressure reading 97 motor drive power 101 pressure above upper limit 94 pressure below lower limit 95 pressure signal missing 96 pump head missing 104 selection valve fuse 98 servo restart failed 103 shutdown 87 solvent zero counter 93 stroke length 107 temperature limit exceeded 100 temperature out of range 99 timeout 86 valve failed 96 wait timeout 108 exchanging active inlet valve 148 1260 Binary Pump VL User Manual active inlet valve 148 130 interface board 159 outlet valve sieve 130 outlet valve 130 152 pistons 130 pump seals 130 purge valve frit 130 purge valve 130 solvent selection valve 156 wash seals 130 external contact cable 188 F fan failed 91 fastest gradient response 71 filter solvent inlet 54 firmware description 192 main system 192 resident system 192 update tool 193 updates 160 193 160 upgade downgrade 160 upgrade downgrade 160 flow accuracy 24 flow connections 42 45 flow precision 24 flowrange 24 flow rate minimum 54 frequency range 23 G gas solubility 54 general error messages 86 gradient formation 24 Index highest injection precision 68 high pressure mixing 10 hints for successful use 54 humidity 23 hydraulic path with solvent selection valve 172 hydraulic path without solvent selection valve 174 hydraul
72. door use only 1260 Binary Pump VL User Manual 23 2 Performance Specifications 24 Site Requirements and Specifications Table 3 Performance Specification Agilent 1260 Infinity Binary Pump VL G1312C Type Specification Comments Hydraulic system Setable flow range Flow range Flow precision Flow accuracy Pressure operating range Pressure pulsation Compressibility compensation Recommended pH range Gradient formation Delay volume Two dual piston in series pumps with servo controlled variable stroke drive floating pistons Set points 0 001 5 mL min in 0 001 mL min increments 0 05 5 0 mL min lt 0 07 RSD or 0 02 min SD whatever is greater based on retention time at constant room temperature 1 or 10 pL min whatever is greater pumping degassed H 0 at 10 MPa 100 bar Operating range up to 40 MPa 400 bar 5880 psi up to 5 mL min lt 2 amplitude typically lt 1 3 or lt 0 3 MPa 3 bar whatever is greater at 1 mL min isopropanol at all pressures gt 1 MPa 10 bar 147 psi User selectable based on mobile phase compressibility 1 0 12 5 solvents with pH lt 2 3 should not contain acids which attack stainless steel High pressure binary mixing 600 900 uL includes 400 pL mixer dependent on back pressure 1260 Binary Pump VL User Manual Table 3 Site Requirements and Specifications 2 Performance Specification Agilen
73. e Exchanging the Outlet Valve 153 9 Maintenance Installation of the Solvent Selection Valve Upgrade Kit A solvent selection valve allows you to choose between four different solvents that can be used with a binary pump The valve switches between two solvents Al and A2 for channel A of the left pump head and two solvents B1 and B2 for channel B of the right pump head When Tools required Description Screwdriver Pozidriv 1 Parts required p n G1381 60000 Description Preparations Applicable modules This kit is compatible to the 1260 Infinity Binary Pumps G1312B and G1312C Solvent Selection Valve Upgrade Kit If required remove solvent tubes from the inlet valves The figures below show a Binary Pump G1312B The kit can be used similarly for the Binary Pump G1312C 1 Remove the front cover sheet by removing both screws 2 Plug in the connector of the solvent selection valve jazza Connector SSV cable Solvent selection valve 154 1260 Binary Pump VL User Manual Maintenance 9 Installation of the Solvent Selection Valve Upgrade Kit indicated screws 3 Install the solvent selection assembly by fixing both yam O 0 0O 0O 4 Connect the outlet for solvent A upper row to the inlet valve of the left pump head Connect the outlet for solvent B lower row to the inlet valve of the right pump head Put the solvent
74. e Upgrade Kit 154 Exchanging the Solvent Selection Valve 156 Exchanging the Optional Interface Board 159 Replacing the Module Firmware 160 10 Parts for Maintenance 161 Pump Head Assembly Without Seal Wash 162 Pump Head Assembly with Seal Wash 164 Outlet Valve 166 Purge Valve Assembly 167 Active Inlet Valve Assembly 168 Accessory Kit 169 Active Seal Wash Option 169 Solvent Cabinet 170 Bottle Head Assembly 171 Hydraulic Path with Solvent Selection Valve 172 Hydraulic Path without Solvent Selection Valve 174 1 Identifying Cables 177 Cable Overview 178 Analog Cables 180 Remote Cables 182 BCD Cables 185 CAN Cable 187 External Contact Cable 188 Agilent Module to PC 189 Agilent 1200 Module to Printer 190 12 Hardware Information 191 Firmware Description 192 Electrical Connections 195 Interfaces 197 Setting the 8 bit Configuration Switch without On board LAN 204 1260 Binary Pump VL User Manual Contents 13 Appendix 209 General Safety Information 210 The Waste Electrical and Electronic Equipment Directive 213 Batteries Information 214 Radio Interference 215 Sound Emission 216 Agilent Technologies on Internet 217 8 1260 Binary Pump VL User Manual 1260 Binary Pump VL User Manual 1 Introduction Introduction to the Binary Pump 10 Overview of the Binary Pump 11 How Does the Binary Pump Work 13 How Does Compressibility Compensation Work 15 How Does Variable Stroke Volume Work 16 Ea
75. e error message is generated Probable cause 1 Defective leak sensor 2 Leak sensor incorrectly routed being pinched by a metal component Leak Sensor Open Error ID 0083 Suggested actions Please contact your Agilent service representative Please contact your Agilent service representative The leak sensor in the module has failed open circuit The current through the leak sensor is dependent on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current falls outside the lower limit the error message is generated Probable cause 1 Leak sensor not connected to the main board 2 Defective leak sensor 3 Leak sensor incorrectly routed being pinched by a metal component 1260 Binary Pump VL User Manual Suggested actions Please contact your Agilent service representative Please contact your Agilent service representative Please contact your Agilent service representative 89 7 90 Error Information Compensation Sensor Open Error ID 0081 The ambient compensation sensor NTC on the main board in the module has failed open circuit The resistance across the temperature compensation sensor NTC on the main board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor increases
76. e greater than 10 bar in low delay volume configuration and 20 bar in standard configuration when pumping distilled water at a rate of 5 mL min with an open purge valve Whenever possible use a minimum flow rate of 5 uL min per solvent channel to avoid crossflow of solvent into the unused pump channel Whenever exchanging the pump seals the purge valve frit should be exchanged too When using buffer solutions flush the system with water before switching it off The seal wash option should be used when buffer solutions with concentrations of 0 1 M or higher are being pumped for long periods of time Check the pump pistons for scratches grooves and dents when changing the piston seals Damaged pistons cause micro leaks and will decrease the lifetime of the seals After changing the piston seals apply the seal wear in procedure see Seal Wear in Procedure on page 147 Place the aqueous solvent on channel A and the organic solvent on channel B The default compressibility settings are set accordingly 1260 Binary Pump VL User Manual Using the Binary Pump 4 Setting up the Pump with the G4208A Instant Pilot Generic operation of the G4208A Instant Pilot is covered in the Agilent Instant Pilot G4208A User s Guide p n G4208 90006 Details about setting up module specific parameters can be found in the Instant Pilot online help The pump parameters are described in detail in Overview on page 56 1260 Binary Pump VL Use
77. e inlet valve 600 bar Figure 21 Active Inlet Valve Assembly 168 1260 Binary Pump VL User Manual Accessory Kit Parts for Maintenance 10 Accessory Kit p n G1311 68755 p n 5062 2461 5063 6527 5181 1519 G1329 87300 G1312 87303 5042 9954 Description Waste tube 5 m reorder pack Tubing assembly i d 6 mm o d 9 mm 1 2 m to waste CAN cable Agilent module to module 1 m StS Capillary 0 17 mm 900 mm pump to thermostatted autosampler StS Capillary 0 17 mm 400 mm pump to injector Tubing clip 2x re order 4 pk Active Seal Wash Option Active Seal Wash Option kit p n G1312 68721 p n 5065 9953 5042 8507 0905 1175 5062 2484 5065 9978 5063 6589 01018 23702 1260 Binary Pump VL User Manual Description Seal wash pump assembly Peristaltic pump cartridge silicone tubing Secondary seal pre installed in support rings Gasket seal wash pack of 6 Silicone tubing 1 mm i d 3 mm o d 5 m re order number Standard seals pack of 2 Insert tool 169 10 Parts for Maintenance Solvent Cabinet Solvent Cabinet Item p n 1 5067 4770 2 5043 0207 3 5065 9954 4 5042 8907 5 9301 1420 9301 1450 7 G1311 60003 Description Solvent Cabinet Kit Name plate 1260 Front panel solvent cabinet Leak pan solvent cabinet Solvent bottle transparent Solvent bottle amber Bottle head assembly Figure 22 Solvent Cabinet Parts 170 1260 Binary Pump VL User Manual Parts for Mai
78. e piston housing pistons not installed and snap the pump housing and piston housing together Note the correct position of the pin on the support ring Pump housing Support ring Piston housing 10 Insert the pistons and carefully press them into the seals Piston 11 Tighten the lock screw Lock screw 144 12 Reinstall the pump head assembly see Reinstalling the Pump Head Assembly on page 145 1260 Binary Pump VL User Manual Maintenance 9 Reinstalling the Pump Head Assembly When When reassembling the pump Tools required p n Description 8710 2411 Hexagonal key 3 mm Hexagonal key 4 mm Parts required p n Description 1 79846 65501 Pump head grease 1 Slide the pump head assembly onto the pump drive Ga yA Pump head screws 2 Using a 4 mm hexagonal key tighten the pump head screws stepwise with increasing torque 1260 Binary Pump VL User Manual 145 9 Maintenance Reinstalling the Pump Head Assembly 3 Reconnect the capillaries at the back of the purge valve holder the pump head adapter and the tube at the active inlet valve Reconnect the active inlet valve connector 146 1260 Binary Pump VL User Manual Maintenance 9 Seal Wear in Procedure CAUTION Seal damage This procedure is required for black PTFE seals standard applications p n 5063 6589 but it will damage the yellow PE seals normal phase applications p n 0905 1420 gt Do not ru
79. eal time requirement LAN The modules have either an interface slot for an LAN card e g Agilent G1369B C LAN Interface or they have an on board LAN interface e g detectors G1315C D DAD and G1365C D MWD This interface allows the control of the module system via a PC with the appropriate control software If an Agilent detector DAD MWD FLD VWD RID is in the system the LAN should be connected to the DAD MWD FLD VWD RID due to higher data load If no Agilent detector is part of the system the LAN interface should be installed in the pump or autosampler RS 232C Serial The RS 232C connector is used to control the module from a computer through RS 232C connection using the appropriate software This connector can be configured with the configuration switch module at the rear of the module Refer to Communication Settings for RS 232C There is no configuration possible on main boards with on board LAN These are pre configured for 19200 baud e 8 data bit with no parity and one start bit and one stop bit are always used not selectable The RS 232C is designed as DCE data communication equipment with a 9 pin male SUB D type connector The pins are defined as 1260 Binary Pump VL User Manual Hardware Information 12 Table 12 RS 232C Connection Table Pin Direction Function 1 In DCD 2 In RxD 3 Out TxD 4 Out DTR 5 Ground 6 In DSR 7 Out RTS 8 In CTS 9 In RI Instrument PC DCD 1 1 DCD
80. ed The visual feedback in the user interface provides an indication that maintenance procedures should be scheduled EMF Counters EMF counters increment with use and can be assigned a maximum limit which provides visual feedback in the user interface when the limit is exceeded Some counters can be reset to zero after the required maintenance procedure Using the EMF Counters The user settable EMF limits for the EMF Counters enable the early maintenance feedback to be adapted to specific user requirements The useful maintenance cycle is dependent on the requirements for use Therefore the definition of the maximum limits need to be determined based on the specific operating conditions of the instrument Setting the EMF Limits The setting of the EMF limits must be optimized over one or two maintenance cycles Initially the default EMF limits should be set When instrument performance indicates maintenance is necessary take note of the values displayed by the EMF counters Enter these values or values slightly less than the displayed values as EMF limits and then reset the EMF counters to zero The next time the EMF counters exceed the new EMF limits the EMF flag will be displayed providing a reminder that maintenance needs to be scheduled 1260 Binary Pump VL User Manual 17 1 Introduction Instrument Layout The industrial design of the module incorporates several innovative features It uses Agilent s E PAC concept for the
81. emote line Error Information The module continually monitors the remote input connectors for status signals A LOW signal input on pin 4 of the remote connector generates the error message Probable cause 1 Leak detected in another module with a CAN connection to the system 2 Leak detected in an external instrument with a remote connection to the system 3 Shut down in an external instrument with a remote connection to the system 4 The degasser failed to generate sufficient vacuum for solvent degassing 1260 Binary Pump VL User Manual Suggested actions Fix the leak in the external instrument before restarting the module Fix the leak in the external instrument before restarting the module Check external instruments for a shut down condition Check the vacuum degasser for an error condition Refer to the Service Manual for the degasser or the 1260 pump that has the degasser built in 7 87 7 88 Error Information Remote Timeout Error ID 0070 A not ready condition is still present on the remote input When an analysis is started the system expects all not ready conditions for example a not ready condition during detector balance to switch to run conditions within one minute of starting the analysis If a not ready condition is still present on the remote line after one minute the error message is generated Probable cause Suggested actions 1 Not ready condition in one of the Ensure the i
82. f solvent A smarter way however is to use the bottle fillings function see Bottle Filling on page 60 SolventA 0 100 Although channel A can be set to 0 it cannot be turned off This channel should be used for the aqueous phase water SolventB off 100 The percentage of channel B is automatically complemented by channel A to give 100 Timetable max number of lines Use the timetable to build solvent gradients flow gradients or combinations of depends on free space both Gradients are always linear Use multiple timetable entries to mimic in pump memory exponential or parabolic gradients Display There are three ways to display the timetable e in tabular form as flow pressure graph as solvent percentage plot Values can only be changed in tabular view 1260 Binary Pump VL User Manual 57 4 Using the Binary Pump Pump Control The pump can be switched between following states On Off or to Standby In Standby the pump motor is still controlled When the pump is switched on from standby it does not re initialize CAUTION Upon initialization the pump ignores the Maximum Flow Gradient value This can result in a rapid and uncontrolled pressure increase gt To prevent harm to the column open the purge valve until the initialization is finished The optional seal wash pump can be controlled by either switching it off using it for a single time or specifying frequency and duration of peri
83. formation 12 Electrical Connections e The CAN bus is a serial bus with high speed data transfer The two connectors for the CAN bus are used for internal module data transfer and synchronization e One analog output provides signals for integrators or data handling systems The interface board slot is used for external contacts and BCD bottle number output or LAN connections The REMOTE connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features such as start stop common shut down prepare and so on e With the appropriate software the RS 232C connector may be used to control the module from a computer through a RS 232C connection This connector is activated and can be configured with the configuration switch e The power input socket accepts a line voltage of 100 240 VAC 10 witha line frequency of 50 or 60 Hz Maximum power consumption varies by module There is no voltage selector on your module because the power supply has wide ranging capability There are no externally accessible fuses because automatic electronic fuses are implemented in the power supply Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations 1260 Binary Pump VL User Manual 195 12 Hardware Information Electrical Connections Rear View of the Module Configuration switch Slot for in
84. g the Blank Nut 113 Running the Pressure Test 114 Evaluating the Results 115 Potential Causes of Pressure Test Failure 116 Leak Test 117 Description 117 Running the Leak Test 119 Evaluating the Results 120 This chapter describes the tests for the module gy Agilent Technologies 111 8 Test Functions and Calibration Pressure Test Description The pressure test is a quick built in test designed to demonstrate the pressure tightness of the system The test involves monitoring the pressure profile while the binary pump runs through a predefined pumping sequence The resulting pressure profile provides information about the pressure tightness of the system Step 1 The test begins with the initialization of both pumpheads After initialization pistons Al and B1 are both at the top of their stroke Next pump A begins pumping solvent with a flow rate of 510 uL min and stroke of 100 uL The binary pump continues to pump until a system pressure of 390 bar bar is reached For this test channel A is active which is directly connected to chamber 2 in channel B see Figure 2 on page 12 To test the pressure tightness of the pump use the leak test see Description on page 117 Step 2 When the system pressure reaches 390 bar bar the binary pump switches off The pressure drop from this point onwards should be no more than 2 bar min 112 1260 Binary Pump VL User Manual Test Functions and Calibration 8 Positioning
85. gents can hold health and safety risks gt When working with these substances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice gt The volume of substances should be reduced to the minimum required for the analysis gt Do not operate the instrument in an explosive atmosphere Electrical shock Repair work at the module can lead to personal injuries e g shock hazard when the cover is opened gt Do not remove the cover of the module gt Only certified persons are authorized to carry out repairs inside the module Personal injury or damage to the product Agilent is not responsible for any damages caused in whole or in part by improper use of the products unauthorized alterations adjustments or modifications to the products failure to comply with procedures in Agilent product user guides or use of the products in violation of applicable laws rules or regulations gt Use your Agilent products only in the manner described in the Agilent product user guides CAUTION Safety standards for external equipment gt If you connect external equipment to the instrument make sure that you only use accessory units tested and approved according to the safety standards appropriate for the type of external equipment 1260 Binary Pu
86. ic system 24 l index adjustment 106 index limit 105 index missing 106 initialization failed 107 initialization 14 14 inlet valve missing 102 installation pump module 36 installation bench space 22 site requirements 19 instrument layout 18 interface board 159 interface cable 38 interfaces 197 internet 217 introduction to the pump 10 L LAN cable 187 leak sensor open 89 leak sensor short 89 leak test evaluation 120 leak test 77 117 leak 92 line frequency 23 219 Index line voltage 23 liquimeter 140 lithium batteries 214 lost CAN partner 88 M ain assemblies overview 128 maintenance feedback 17 replacing firmware 160 160 message ignition without cover 92 92 remote timeout 88 minimum flow rate 54 missing pressure reading 97 missing parts 29 mixer 11 motor drive power 101 non operating altitude 23 non operating temperature 23 0 operating Altitude 23 operating temperature 23 optimization stack configuration 31 outlet valve 130 152 166 overview pump 11 P packaging damaged 28 parts identification cables 177 parts 220 active inlet valve 168 bottle head assembly 171 damaged 29 hydraulic path with solvent selection valve 172 hydraulic path without solvent selection valve 174 missing 29 outlet valve 166 solvent cabinet 170 performance specification 24 pH range 24 physical specifications 23 piston 13 130 power consumption 23 power supply indicator
87. ies APG Analytical Products Group remote connector to be connected to Agilent modules The other end depends on the instrument to be connected to Agilent Module to 3396A Integrators p n 03394 60600 Pin 3396A Pin Agilent Signal Name Active module TTL E 9 1 White Digital ground NC 2 Brown Prepare run Low 80 7 3 3 Gray Start Low x NC 4 Blue Shut down Low oO e o gt NC 5 Pink Not connected le NC 6 Yellow Power on High 5 14 7 Red Ready High 1 8 Green Stop Low NC 9 Black Start request Low 13 15 Not connected Agilent Module to 3396 Series II 3395A Integrators Use the cable Agilent module to 3396A Series I integrators p n 03394 60600 and cut pin 5 on the integrator side Otherwise the integrator prints START not ready 1260 Binary Pump VL User Manual Identifying Cables 11 Agilent Module to 3396 Series III 3395B Integrators p n 03396 61010 Pin 33XX Pin Agilent Signal Name Active module TTL 9 1 White Digital ground NC 2 Brown Prepare run Low 80 A 3 3 Gray Start Low NC 4 Blue Shut down Low e o NC 5 Pink Not connected le NC 6 Yellow Power on High 14 7 Red Ready High 4 8 Green Stop Low NC 9 Black Start request Low 13 15 Not connected Agilent Module to Agilent 35900 A D Converters p n 5061 3378 Pin 35900 A D Pin Agilent Signal Name Active module TTL
88. ilters in solvent reservoirs for avoiding leakages 1260 Binary Pump VL User Manual 9 133 9 Maintenance 1 Using a 1 4 inch wrench disconnect the pump outlet capillary from the purge valve Disconnect the waste tube Beware of leaking solvents due to hydrostatic pressure Purge valve Waste tube 2 Using the 14 mm wrench unscrew the purge valve and remove it from the purge valve holder 3 Remove the seal cap from the purge valve 4 Using a pair of tweezers or a toothpick remove the frit Valve body PTFE frit with groove QL I oY Seal cap a 5 Place a new frit into the purge valve with the orientation of the frit as shown below slit in frit points to the front Reinstall the seal cap including the gold seal Before reinstallation always check the gold seal in the seal cap A deformed seal cap should be exchanged Next Steps 6 Insert the purge valve into the purge valve holder and orient the waste outlet nozzle downward 7 Tighten the purge valve and reconnect outlet capillary and waste tubing 134 1260 Binary Pump VL User Manual Maintenance 9 Removing the Pump Head Assembly When Exchanging pump seals Exchanging pistons Exchanging seals of the seal wash option Tools required p n Description 8710 0510 Wrench open 1 4 5 16 inch 8710 2411 Hex key 3 mm12 cm long 8710 2392 Hex key 4 mm15 cm long T handle 5023 0240 Hex driver 4 slitted Prepar
89. inary Pump VL User Manual Error Information 7 Missing Pressure Reading Error ID 2054 The pressure readings read by the pump ADC analog digital converter are missing The ADC reads the pressure signal of from the damper every Ims If the readings are missing for longer than 10 s the error message is generated Probable cause Suggested actions 1 Damper disconnected Please contact your Agilent service representative 2 Defective damper Please contact your Agilent service representative 3 Defective main board Please contact your Agilent service representative Pump Configuration Error ID 2060 At switch on the pump has recognized a new pump configuration The pump is assigned its configuration at the factory If the active inlet valve and pump encoder of channel B are disconnected and the pump is rebooted the error message is generated However the pump will function as an isocratic pump in this configuration The error message reappears after each switch on Probable cause Suggested actions 1 Active inlet valve and pump encoder of Reconnect the active inlet valve and pump encoder channel B disconnected of channel B 1260 Binary Pump VL User Manual 97 7 98 Error Information Electronic Fuse of SSV Error ID 2049 Following errors can only occur if a solvent selection valve is used The internal number in the error message is linked either to channels A or B as shown in the following table Valve Fu
90. ing up the Agilent 1260 Infinity Binary Pump VL G1312C with the Instrument Control Interface 56 Solvent Information 62 Prevent Blocking of Solvent Filters and Algae Growth 64 Algae Growth in HPLC Systems 65 1260 Binary Pump VL User Manual Contents Optimizing Performance 67 When to Use a Vacuum Degasser 68 When to Use the Active Seal Wash Option 69 When to Use Alternative Seals 70 When to Remove the Static Mixer 71 How to Optimize the Compressibility Compensation Setting 72 Troubleshooting and Diagnostics 75 Overview of the Pump s Indicators and Test Functions 76 Status Indicators 78 User Interfaces 80 Agilent Lab Advisor Software 81 Error Information 83 What are Error Messages 85 General Error Messages 86 Module Error Messages 93 Test Functions and Calibration 111 Pressure Test 112 Leak Test 117 Maintenance 125 Introduction to Maintenance and Repair 126 Warnings and Cautions 127 Overview of Maintenance 128 Cleaning the Module 131 Checking and Cleaning the Solvent Filter 132 Exchanging the Purge Valve Frit or the Purge Valve 133 Removing the Pump Head Assembly 135 Maintenance of a Pump Head without Seal Wash 137 Maintenance of a Pump Head with Seal Wash 141 Reinstalling the Pump Head Assembly 145 Seal Wear in Procedure 147 Exchanging the Active Inlet Valve or its Cartridge 148 Exchanging the Outlet Valve 152 1260 Binary Pump VL User Manual Contents Installation of the Solvent Selection Valv
91. intenance of a Pump Head without Seal Wash 4 Using the steel side of the insert tool carefully remove the seal from the pump housing Remove wear retainers if still present Insert tool Piston seal Pump housing 5 Using the plastic side of the insert tool insert the new seals into the pump head and press them firmly in position Insert tool 6 Place a seal wash gasket in the recess of the support ring Put the seal holder on top of the gasket Seal holder Gasket Support ring 7 Reassemble the pump head assembly Note the correct position of the pin on the support ring Pump housing Support rings Piston housing 1260 Binary Pump VL User Manual 139 9 Maintenance Reset the seal wear counter and liquimeter in the Agilent Lab Advisor 8 Insert the pistons and carefully press them into the seals Pistons 9 Tighten the lock screw Lock screw 140 Next Steps 10 Reinstall the pump head assembly see Reinstalling the Pump Head Assembly on page 145 11 If a standard seal has been installed run the seal wear in procedure see Seal Wear in Procedure on page 147 12 For the normal phase seal the purge valve frit should be replaced see Exchanging the Purge Valve Frit or the Purge Valve on page 133 1260 Binary Pump VL User Manual Maintenance 9 Maintenance of a Pump Head with Seal Wash When In case of maintenance
92. ions 26 1260 Binary Pump VL User Manual 1260 Binary Pump VL User Manual ece 3 Installing the Pump Unpacking the Binary Pump 28 Damaged Packaging 28 Delivery Checklist 29 Optimizing the Stack Configuration 31 One Stack Configuration 31 Two Stack Configuration 34 Installing the Binary Pump 36 Connecting Modules and Control Software 39 Connecting Agilent 1260 Infinity Modules 39 Connecting an Agilent 1260 Infinity Vacuum Degasser 40 Connecting Control Software and or G4208 A Instant Pilot 41 Flow Connections of the Binary Pump with Optional Solvent Selection Valve 42 Flow Connections of the Binary Pump Without Solvent Selection Valve 45 Priming the System 48 Initial Priming 48 Regular Priming 50 Changing Solvents 51 This chapter gives information about the preferred stack setup for your system and the installation of your module a Agilent Technologies 27 3 Installing the Pump Unpacking the Binary Pump Damaged Packaging If the delivery packaging shows signs of external damage please call your Agilent Technologies sales and service office immediately Inform your service representative that the instrument may have been damaged during shipment CAUTION Defective on arrival problems If there are signs of damage please do not attempt to install the module Inspection by Agilent is required to evaluate if the instrument is in good condition or damaged gt Notify your Agilent sales and service office abo
93. is manual For details refer to the Agilent Lab Advisor software help files The Instrument Utilities is a basic version of the Lab Advisor with limited functionality required for installation use and maintenance No advanced repair troubleshooting and monitoring functionality is included 1260 Binary Pump VL User Manual 81 6 Troubleshooting and Diagnostics Agilent Lab Advisor Software 82 1260 Binary Pump VL User Manual 1260 Binary Pump VL User Manual 7 Error Information What are Error Messages 85 General Error Messages 86 Timeout 86 Shutdown 87 Remote Timeout 88 Lost CAN Partner 88 Leak Sensor Short 89 Leak Sensor Open 89 Compensation Sensor Open 90 Compensation Sensor Short 90 Fan Failed 91 Leak 92 OpenCover 92 Module Error Messages 93 Restart Without Cover 93 Solvent Zero Counter 93 Pressure Above Upper Limit 94 Pressure Below Lower Limit 95 Pressure Signal Missing 96 Valve Failed 96 Missing Pressure Reading 97 Pump Configuration 97 Electronic Fuse of SSV 98 AIV Fuse 99 Temperature Out of Range 99 Temperature Limit Exceeded 100 Motor Drive Power 101 Encoder Missing 102 oe Agilent Technologies 83 7 84 Error Information Inlet Valve Missing 102 Servo Restart Failed 103 Pump Head Missing 104 Index Limit 105 Index Adjustment 106 Index Missing 106 Stroke Length 107 Initialization Failed 107 Wait Timeout 108 Electronic fuse of SSV 109 This chapter describes the meaning of error messages and
94. ithout Solvent Selection Valve on page 45 7 Press the power switch to turn on the module NOTE The power switch stays pressed in and a green indicator lamp in the power switch is on when the module is turned on When the line power switch stands out and the green light is off the module is turned off 8 Purge the binary pump see Initial Priming on page 48 NOTE The pump was shipped with default configuration settings To change these settings see Setting the 8 bit Configuration Switch without On board LAN on page 204 38 1260 Binary Pump VL User Manual Installing the Pump 3 Connecting Modules and Control Software WARNING Use of unsupplied cables Using cables not supplied by Agilent Technologies can lead to damage of the electronic components or personal injury gt Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Connecting Agilent 1260 Infinity Modules 1 Place the individual modules in a stack configuration as shown in Figure 4 on page 32 2 Ensure the power switches on the front of the modules are OFF switches stand out 3 Plug a CAN cable into the CAN connector at the rear of the respective module except vacuum degasser 4 Connect the CAN cable to the CAN connector of the next module see Figure 5 on page 33 5 Press in the power switches to turn on the modules 1260 Binary
95. k out The handling of toxic and hazardous solvents and reagents can carry health risks gt Observe appropriate safety procedures for example wear goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used 132 Cleaning the Solvent Filter 1 Remove the blocked solvent filter from the bottle head assembly and place it in a beaker with concentrated nitric acid 35 for one hour 2 Thoroughly flush the filter with LC grade water remove all nitric acid some columns can be damaged by concentrated nitric acid check with pH indicator 3 Reinstall the filter 1260 Binary Pump VL User Manual Maintenance Exchanging the Purge Valve Frit or the Purge Valve When Tools required OR Parts required Preparations Frit when piston seals are exchanged or when contaminated or blocked pressure drop of gt 10 bar across the frit at a flow rate of 5 mL min of water with purge valve opened Purge valve if internally leaking p n Description 8710 0510 Wrench open 1 4 5 16 inch 8710 1924 Wrench open 14 mm Pair of tweezers Toothpick p n Description 01018 22707 PTFE frits pack of 5 G1312 60061 Purge valve 1260 5067 4728 Seal cap OPTIONAL Switch off pump at the main power switch Remove the front cover Use an optional solvent shutoff valve or lift up solvent f
96. k test for verification Seal life time shorter than normally expected check plungers while changing the seals Leaks at lower pump head side loss of wash solvent Error condition indicated by red status indicator 130 1260 Binary Pump VL User Manual Maintenance 9 Cleaning the Module To keep the module case clean use a soft cloth slightly dampened with water or a solution of water and mild detergent WARNING Liquid dripping into the electronic compartment of your module can cause shock hazard and damage the module gt Do not use an excessively damp cloth during cleaning gt Drain all solvent lines before opening any connections in the flow path 1260 Binary Pump VL User Manual 131 9 Maintenance Checking and Cleaning the Solvent Filter When Parts required Preparations CAUTION If solvent filter is blocked Description Concentrated nitric acid 35 LC grade water Beaker Remove solvent inlet tube from the adapter at the AIV Small particles can permanently block the capillaries and valves of the module Damage of the module gt Always filter solvents gt Never use the module without solvent inlet filter If the filter is in good condition the solvent will freely drip out of the solvent tube hydrostatic pressure If the solvent filter is partly blocked only very little solvent will drip out of the solvent tube When opening capillary or tube fittings solvents may lea
97. l 117 118 Test Functions and Calibration Plateau 2 Piston B2 delivers with a flow rate of 3 uL min for 30 s Ramp 5 Piston A1 delivers 50 uL min for approximately 8 s Plateau 3 Piston A1 delivers with a flow rate of 3 uL min for 30 s Ramp 6 Piston B1 delivers 50 uL min for approximately 7 s Plateau 4 Piston B1 delivers with a flow rate of 3 uL min for approximately 30 s At the end of the fourth plateau the test is finished and the pump switches off 1260 Binary Pump VL User Manual Test Functions and Calibration 8 Running the Leak Test When Tools required Parts required Preparations If problems with the pump are suspected Description Wrench 1 4 inch p n Description 1 G1313 87305 Restriction Capillary 1 01080 83202 Blank nut 1 500 mL Isopropanol Place two bottles of LC grade isopropyl alcohol in channels A and B If a solvent selection valve is installed place the LC grade isopropanol in channels A2 and B2 Make absolutely sure that all parts of the flow path that are part of the test are very thoroughly flushed with isopropanol before starting to pressurize the system Any trace of other solvents or the smallest air bubble inside the flow path definitely will cause the test to fail Running the test from the Agilent Lab Advisor 1 Select the Leak Test from the Test Selection menu 2 Start the test and follow the instructions Make sure to release the pressure by slowly opening
98. l in optional Exchange the rotor seal valve 116 1260 Binary Pump VL User Manual Test Functions and Calibration 8 Leak Test Description The leak test is a built in troubleshooting test designed to demonstrate the leak tightness of the binary pump The test involves monitoring the pressure profile as the binary pump runs through a predefined pumping sequence The resulting pressure profile provides information about the pressure tightness and operation of the binary pump components see Figure 2 on page 12 Ramp 1 The test begins with the initialization of both pumps After initialization pistons Al and B1 are both at the top of their stroke Next the pump begins pumping solvent with a flow rate of 150 uL min stroke of 100 uL and a composition of 51 A 49 B Both pumps deliver for one complete pump cycle At the end of this step pistons Al and B1 are at the top of their stroke Ramp 2 The pump continues pumping solvent with a flow rate of 150 uL min Channel A delivers for one pump cycle first piston A2 delivers then piston A1 followed by channel B piston B2 then piston B1 both channels with a stroke of 20 uL Ramp 3 Just before the start of the first plateau piston A2 delivers with a flow rate of 50 uL min for approximately 8 s Plateau 1 At plateau 1 piston A2 delivers with a flow rate of 3 uL min for 30 s Ramp 4 Piston B2 delivers 50 uL min for approximately 8 s 1260 Binary Pump VL User Manua
99. l wash 0890 1764 Tubing seal wash 5 0905 1175 Wash seal PTFE 6 5062 2484 Gasket seal wash pack of 6 7 5042 8952 Seal holder 8 G1312 67300 Capillary outlet valve to piston 2 9 5063 6589 Piston seal PTFE carbon filled black pack of 2 default 10 G1311 25200 Pump chamber housing 11 0515 0175 Mounting screw for manual purge valve holder M4 20 mm long 12 G1312 23200 Holder for manual purge valve 13 G1312 60061 Purge valve 1260 14 G1312 60067 Outlet valve 1220 1260 15 5042 1303 Lock screw 16 G1312 60025 Active inlet valve body without cartridge G1312 60020 Cartridge for active inlet valve 600 bar 17 G1312 23201 Adapter 18 0515 2118 Pump head screw M5 60 mm 164 1260 Binary Pump VL User Manual Parts for Maintenance 10 Pump Head Assembly with Seal Wash Figure 18 Pump head assembly with seal wash option 1260 Binary Pump VL User Manual 165 10 Parts for Maintenance Outlet Valve Outlet Valve p n Description G1312 60067 Outlet valve 1220 1260 Figure 19 Outlet Valve 166 1260 Binary Pump VL User Manual Parts for Maintenance 10 Purge Valve Assembly Item p n Description 1 G1312 60061 Purge valve 1260 2 01018 22707 PTFE frits pack of 5 3 5067 4728 Seal cap Figure 20 Purge Valve Assembly 1260 Binary Pump VL User Manual 167 10 Parts for Maintenance Active Inlet Valve Assembly Item p n Description 1 G1312 60025 Active inlet valve body without cartridge 2 G1312 60020 Cartridge for activ
100. ling the Pump 3 1 Remove the front cover by pressing the snap fasteners on both sides w N 4 oa Figure 10 Removing the Front Cover Place the solvent cabinet on top of the module Set the four bottles into the solvent cabinet and screw a bottle head assembly onto each bottle Connect the solvent tubes from the bottle head assemblies to the inlet connectors Al A2 B1 and B2 of the solvent selection valve and label the tubes accordingly Fix the tubes in the clips of solvent cabinet and binary pump Using a piece of sanding paper connect the waste tubing to the purge valve and place it into your waste system If the binary pump is not part of an Agilent 1260 Infinity system stack or placed on the bottom of a stack connect the corrugated waste tube to the waste outlet of the pump leak handling system Connect the outlet capillary binary pump to injection device to the outlet of the purge valve 1260 Binary Pump VL User Manual 43 3 Installing the Pump Flow Connections of the Binary Pump with Optional Solvent Selection Valve 8 Prime your system before first use see Initial Priming on page 48 Bottle head assembly Solvent cabinet Degasser Channel A1 Channel A2 Mixer Active seal wash option Mixing chamb Purge valve ixing chamber leew Pump head A ST eS Pump head B Active inlet valve A B mm mt am Active inlet valve B iS Waste tubi
101. ll met within the premises 1260 Binary Pump VL User Manual 215 13 Appendix Sound Emission Manufacturer s Declaration This statement is provided to comply with the requirements of the German Sound Emission Directive of 18 January 1991 This product has a sound pressure emission at the operator position lt 70 dB e Sound Pressure Lp lt 70 dB A e At Operator Position e Normal Operation e According to ISO 7779 1988 EN 27779 1991 Type Test 216 1260 Binary Pump VL User Manual Appendix 13 Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at http www agilent com Select Products Chemical Analysis It will provide also the latest firmware of the modules for download 1260 Binary Pump VL User Manual 217 Index Index 8 bit configuration switch without On Board LAN 204 A active inlet valve 130 148 148 168 active seal wash 10 69 adapter 46 148 Agilent Diagnostic software 81 Agilent Lab Advisor software 81 Agilent Lab Advisor 81 Agilent on internet 217 AIV fuse 99 algae growth 64 algae 54 alternative seal material 70 ambient non operating temperature 23 ambient operating temperature 23 analog output 25 analog signal 201 analog cable 180 apgremote 202 assembly bottle head 171 AUTO mode 16 AUX output 40 B ball screw drive 13 battery safety information 214 218 BCD board 159 cable 18
102. lve Frit or the Purge Valve on page 133 Exchanging the Purge Valve Frit or the Purge Valve on page 133 Exchanging the Solvent Selection Valve on page 156 Exchanging the pump seals see Maintenance of a Pump Head without Seal Wash on page 137 or Maintenance of a Pump Head with Seal Wash on page 141 Exchanging pistons see Maintenance of a Pump Head without Seal Wash on page 137 or Maintenance of a Pump Head with Seal Wash on page 141 Exchanging the wash seals see Maintenance of a Pump Head with Seal Wash on page 141 Exchanging the Optional Interface Board on page 159 If solvent filter is blocked If internally leaking If internally leaking If internally leaking If the frit shows indication of contamination or blockage If internally leaking If pump performance indicates seal wear If scratched When seals show indication of leaks If defective Gradient performance problems intermittent pressure fluctuations Pressure ripple unstable run leak test for verification Pressure ripple unstable run leak test for verification Solvent dripping out of waste outlet when valve closed A pressure drop of gt 10 bar across the frit 5 ml min H20 with purge valve open indicates blockage Error messages Valve failed or Valve Fuse are generated Leaks at lower pump head side unstable retention times pressure ripple unstable run lea
103. mber for example 102 is revision 1 02 XXX is the build number of the firmware For instructions on firmware updates refer to section Replacing Firmware in chapter Maintenance or use the documentation provided with the Firmware Update Tools Update of main system can be done in the resident system only Update of the resident system can be done in the main system only Main and resident firmware must be from the same set Main FW update Resident System Main System Resident FW Update Figure 26 Firmware Update Mechanism 1260 Binary Pump VL User Manual 193 12 Hardware Information 194 Some modules are limited in downgrading due to their main board version or their initial firmware revision For example a G1315C DAD SL cannot be downgraded below firmware revision B 01 02 or to a A xx xx Some modules can be re branded e g G1314C to G1314B to allow operation in specific control software environments In this case the feature set of the target type are use and the feature set of the original are lost After re branding e g from G1314B to G1314C the original feature set is available again All these specific informations are described in the documentation provided with the firmware update tools The firmware update tools firmware and documentation are available from the Agilent web http www chem agilent com EN US SUPPORT DOWNLOADS FIRMWARE Pages LC aspx 1260 Binary Pump VL User Manual Hardware In
104. mp VL User Manual 127 pump heads and its parts do require normal maintenance for example seal Figure 15 on page 128 shows the main assemblies of the binary pump The exchange and can be accessed from the front simple repairs LD Z D SLL ID N S PNAN LIR Z Y is OVS aN ga Ww a RL 1260 Binary Pump VL User Manual Overview of Maintenance Procedures Figure 15 Maintenance Overview of Maintenance 128 Maintenance 9 1 Solvent selection valve see Installation of the Solvent Selection Valve Upgrade Kit on page 154 2 Active inlet valve see Exchanging the Active Inlet Valve or its Cartridge on page 148 3 Purge valve see Exchanging the Purge Valve Frit or the Purge Valve on page 133 4 Outlet valve see Exchanging the Outlet Valve on page 152 5 Pump head see Maintenance of a Pump Head without Seal Wash on page 137 or Maintenance of a Pump Head with Seal Wash on page 141 1260 Binary Pump VL User Manual 129 9 Maintenance Simple Repairs The procedures described in this section can be done with the binary pump in place in the system stack Table 10 Simple Repair Procedures Procedure Typical Condition Notes Checking and Cleaning the Solvent Filter on page 132 Exchanging the Active Inlet Valve or its Cartridge on page 148 Exchanging the Outlet Valve on page 152 Exchanging the Purge Va
105. n damage to the product or loss of important data Do not proceed beyond a CAUTION notice until the indicated condi tions are fully understood and met A WARNING notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly performed or adhered to could result in personal injury or death Do not proceed beyond a WARNING notice until the indi cated conditions are fully under stood and met 1260 Binary Pump VL User Manual In This Guide In This Guide This manual covers the Agilent 1260 Infinity Quaternary Pump VL G1311C 1 Introduction This chapter gives an introduction to the module instrument overview and internal connectors 2 Site Requirements and Specifications This chapter provides information on environmental requirements physical and performance specifications 3 Installing the Pump This chapter gives information about the preferred stack setup for your system and the installation of your module 4 Using the Binary Pump This chapter provides information for optimized usage of the binary pump 5 Optimizing Performance This chapter gives hints on how to optimize the performance or use additional devices 6 Troubleshooting and Diagnostics Overview about the troubleshooting and diagnostic features 7 Error Information This chapter describes the meaning of error messages and provides information on probable causes and suggested
106. n the seal wear in procedure if PE seals are installed in the pumphead 1 Puta bottle with 100 ml of isopropanol in the solvent cabinet and place the solvent intake filter of the pump head you want to wear in into this bottle 2 Screw the PEEK adapter 1 4 28 to 10 32 p n 0100 1847 onto the active inlet valve and connect the inlet tube from the bottle head directly to it 3 Connect the Restriction capillary p n 5022 2159 to the purge valve Connect its other end to a waste container 4 Open the purge valve and purge the system for 5 min with isopropanol at a flow rate of 2 mL min 5 Close the purge valve set the flow to a value that gives a pressure of 350 bar Pump 15 min at this pressure to wear the seals in The pressure can be monitored on the analog output connector of the pump with the Instant Pilot chromatographic data system or any other controlling device connected to your pump 6 Turn OFF the pump slowly open the purge valve to release the pressure from the system disconnect the restriction capillary and reconnect the outlet capillary to the purge valve Reconnect the intake tubing to the solvent selection valve and the connecting tube from the solvent selection valve if installed to the AIV 7 Purge your system with the solvent used for your next application 1260 Binary Pump VL User Manual 147 9 Maintenance Exchanging the Active Inlet Valve or its Cartridge When Tools required Parts required Prepar
107. n valve 6 Pump 30 mL isopropanol to remove residual air bubbles 7 Switch to the next solvent channel and repeat steps 5 and 6 until all channels have been purged 8 Turn the flow off and close the purge valve 1260 Binary Pump VL User Manual 49 3 Installing the Pump Regular Priming When When the pumping system has been turned off for a certain time for example overnight air will rediffuse into the solvent channel between the vacuum degasser and the pump If solvents containing volatile components are left in the degasser without flow for a prolonged period there will be a slight loss of the volatile components Preparations Switch the system on The purge tool of the LabAdvisor or Instrument Utilities can be used for automatically purging the pump 1 Open the purge valve of your pump by turning it counterclockwise and set the flow rate to 5 mL min Flush the vacuum degasser and all tubes with at least 10 mL of solvent Repeat step 1 and 2 for the other channel s of the pump Set the required composition and flow rate for your application and close the purge valve 5 Pump for approximately 10 minutes before starting your application 50 1260 Binary Pump VL User Manual Installing the Pump 3 Changing Solvents When Parts required Preparations CAUTION When the solvent of a channel is to be replaced by another solvent that is not compatible solvents are immiscible or one solvent contains a buffer it is
108. nd accuracy For details please refer to How to Optimize the Compressibility Compensation Setting on page 72 1260 Binary Pump VL User Manual 59 4 Using the Binary Pump Data Curves The binary pump provides the possibility to store the following operational data in the data file of the Agilent data system e Solvent percentage for each channel pump flow pressure The pressure data curve is generated from the pressure sensor readings while A B and flow are calculated from the method settings of the pump For details please refer to the online help or manual of your instrument control software Bottle Filling CAUTION The pump offers a powerful feature to monitor the liquid level in the solvent bottles With total bottle volume and initial filling volume set correctly the pump subtracts the consumed volume continuously from the initial value and stops the pump and method sequence execution before the system runs dry or an analysis is corrupted The bottle filling feature fails if multiple solvent inlets are put into one solvent bottle gt In that case implement a minimum pressure limit see Table 5 on page 57 to avoid that the pump runs dry when solvents are empty 60 Table 7 on page 61 lists the available bottle filling parameters 1260 Binary Pump VL User Manual Table7 Bottle Filling Parameters Using the Binary Pump 4 Parameter Limits Description Total Volume 0 1000
109. ng Leak drain Outlet capillary to autosampler annel B Channel B1 Solvent selction valve Figure 11 Binary Pump with Solvent Selection Valve 44 1260 Binary Pump VL User Manual Installing the Pump 3 Flow Connections of the Binary Pump Without Solvent Selection Valve Parts required p n Description 1 Other modules 1 G1311 68755 Accessory Kit 2 wrenches 1 4 5 16 inch for capillary connections Preparations Pump is installed in the LC system WARNING When opening capillary or tube fittings solvents may leak out The handling of toxic and hazardous solvents and reagents can carry health risks gt Observe appropriate safety procedures for example wear goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used 1260 Binary Pump VL User Manual 45 3 Installing the Pump 1 Remove the front cover by pressing the snap fasteners on both sides Figure 12 Removing the Front Cover 2 Place the solvent cabinet on top of the binary pump Place the bottles into the solvent cabinet and place a bottle head assembly into each bottle 4 Connect the solvent tubes from the bottle head assemblies to the inlet adapters of the active inlet valves Fix the tubes in the clips of solvent cabinet and binary pump 5 Using a piece of sanding paper connect the waste tubing to the purge valve and pla
110. ng the pump e optimizing performance troubleshooting and diagnostics e maintenance e parts and materials identifying cables e hardware information e safety Agilent Technologies 2008 2010 2012 Printed in Germany 02 2012 G1312 90008 EE Agilent Technologies
111. ning capillary or tube fittings solvents may leak out The handling of toxic and hazardous solvents and reagents can carry health risks gt Observe appropriate safety procedures for example wear goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used 48 The purge tool of the LabAdvisor or Instrument Utilities can be used to purge the pump automatically If the pump is not able to draw in the solvent from the bottles use a syringe to move the solvent manually through tubing and degasser 1260 Binary Pump VL User Manual Installing the Pump 3 When priming the vacuum degasser with a syringe the solvent is drawn through the degasser tubes very quickly The solvent at the degasser outlet will therefore not be fully degassed Pump for approximately 10 minutes at your desired flow rate before starting an analysis This will allow the vacuum degasser to properly degas the solvent in the degasser tubes Open the purge valve of the pump Set the flow rate to 5 mL min Select channel Al Turn the flow on ao A WH Observe if the solvent in the tubing of channel A1 is advancing towards the pump If it isn t disconnect the solvent tubing from the solvent selection valve attach a syringe with a syringe adapter and pull the liquid through the degasser Reattach the tubing to the solvent selectio
112. notconnected 15 5V Low 185 1260 Binary Pump VL User Manual 11 Identifying Cables Agilent Module to 3396 Integrators p n 03396 60560 Pin 3396 Pin Agilent Signal Name BCD Digit module n 1 1 BCD 5 20 ae 2 2 BCD 7 80 K 3 3 BCD 6 40 4 4 BCD 4 10 g 5 5 BCDO 1 6 6 BCD 3 8 z 7 7 BCD 2 4 8 8 BCD 1 2 9 9 Digital ground NC 15 5V Low 186 1260 Binary Pump VL User Manual CAN Cable Identifying Cables 11 Both ends of this cable provide a modular plug to be connected to Agilent modules CAN or LAN connectors CAN Cables p n 5181 1516 5181 1519 LAN Cables p n 5023 0203 5023 0202 1260 Binary Pump VL User Manual Description CAN cable Agilent module to module 0 5 m CAN cable Agilent module to module 1 m Description Cross over network cable shielded 3 m for point to point connection Twisted pair network cable shielded 7 m for point to point connection 187 11 Identifying Cables External Contact Cable 188 5 10 915 6 11 One end of this cable provides a 15 pin plug to be connected to Agilent modules interface board The other end is for general purpose Agilent Module Interface Board to general purposes p n G1103 61611 Color Pin Agilent Signal Name module White 1 EXT 1 a Brown 2 EXT 1 x Green 3 EXT 2 Yellow 4 EXT 2 Grey 5 EXT 3 Pink 6 EXT 3 Blue 7 EXT 4 Red 8 EXT 4 Black 9 Not connected Violet 10 Not connected
113. nstrument showing the not ready instruments connected to the remote line condition is installed correctly and is set up correctly for analysis 2 Defective remote cable Exchange the remote cable 3 Defective components in the instrument Check the instrument for defects refer to the showing the not ready condition instrument s documentation Lost CAN Partner Error ID 0071 During an analysis the internal synchronization or communication between one or more of the modules in the system has failed The system processors continually monitor the system configuration If one or more of the modules is no longer recognized as being connected to the system the error message is generated Probable cause Suggested actions 1 CAN cable disconnected Ensure all the CAN cables are connected correctly Ensure all CAN cables are installed correctly 2 Defective CAN cable Exchange the CAN cable 3 Defective main board in another module Switch off the system Restart the system and determine which module or modules are not recognized by the system 1260 Binary Pump VL User Manual Leak Sensor Short Error ID 0082 Error Information 7 The leak sensor in the module has failed short circuit The current through the leak sensor is dependent on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current increases above the upper limit th
114. ntenance 10 Bottle Head Assembly Item p n Description 1 9301 1450 Solvent bottle amber 2 9301 1420 Solvent bottle transparent 3 G1311 60003 Bottle head assembly 4 5063 6598 Ferrules with lock ring 10 Pk 5 5063 6599 Tube screw 10 Pk 6 5062 2483 Solvent tubing 5 m 7 5062 8517 Inlet filter adapter 4 Pk 8 5041 2168 Solvent inlet filter 20 um pore size CN Gp ts SH EN A A12 a 57 Figure 23 Bottle Head Assembly Parts 1260 Binary Pump VL User Manual 171 10 Parts for Maintenance Hydraulic Path with Solvent Selection Valve Item p n Description 1 G1312 67307 Capillary damper to mixer 2 G1312 87330 Mixer 3 G1312 67308 Capillary mixer to purge valve 4 G1312 67300 Capillary outlet valve to piston 2 5 G1312 67304 Capillary mixing chamber to damper inlet 6 G1312 67302 Capillary channel A and B pump head outlet to mixing chamber included 2x 7 G1311 67304 Connection tube 8 5062 2461 Waste tube 5 m reorder pack 9 G1312 67305 Outlet capillary pump to injector OR 9 G1329 87300 Capillary pump to thermostattable autosampler G1312 67301 Capillary damper to purge valve Capillary needed when pump used without mixer installed G1311 60003 Bottle head assembly 172 1260 Binary Pump VL User Manual Parts for Maintenance 10 Hydraulic Path with Solvent Selection Valve Bay iS SAW Sages les SAT sya oS C Figure 24 Hydraulic path with solvent selection valve and active se
115. nual Using the Binary Pump 4 Solutions of organic acids acetic acid formic acid and so on in organic solvents For example a 1 solution of acetic acid in methanol will attack steel Solutions containing strong complexing agents for example EDTA ethylene diamine tetra acetic acid e Mixtures of carbon tetrachloride with 2 propanol or THF 1260 Binary Pump VL User Manual 63 4 Using the Binary Pump Prevent Blocking of Solvent Filters and Algae Growth 64 Contaminated solvents or algae growth in the solvent bottle will reduce the lifetime of the solvent filter and will influence the performance of the module This is especially true for aqueous solvents or phosphate buffers pH 4 to 7 The following suggestions will prolong lifetime of the solvent filter and will maintain the performance of the module Use a sterile if possible amber solvent bottle to slow down algae growth Filter solvents through filters or membranes that remove algae Exchange solvents every two days or refilter If the application permits add 0 0001 0 001 M sodium azide to the solvent Place a layer of argon on top of your solvent Avoid exposure of the solvent bottle to direct sunlight Never use the system without solvent filter installed 1260 Binary Pump VL User Manual Using the Binary Pump 4 Algae Growth in HPLC Systems The presence of algae in HPLC systems can cause a variety of problems that may be incorrectly diagnosed
116. odic wash intervals 58 1260 Binary Pump VL User Manual Using the Binary Pump 4 Auxiliary Pump Parameters The auxiliary pump parameters are pre set to fit most applications Adjustments should only be made when required Table 6 on page 59 shows the available auxiliary parameters with their default values CAUTION Upon initialization the pump ignores the Maximum Flow Gradient value This can result in a rapid and uncontrolled pressure increase gt To prevent harm to the column open the purge valve until the initialization is finished Table6 Auxiliary pump parameters Parameter Limits Description Maximum Flow 0 1 100 mL min Gradient default 100 mL min Minimum Stroke 20 100 pL default Auto Compressibility 0 150 10 5 bar default 50 10 bar With this parameter flow rate changes can be ramped up and down slowly to avoid pressure shocks to the column The default value is 100 mL min which in fact turns the function off The volume one pump piston delivers per stroke In general a smaller stroke volume results in lower pump ripple The Auto setting adjusts the strokes dynamically to the lowest possible value The strokes can be set individually for pump heads A and B The compressibility defines the volume change of a compressed liquid This parameter is used for the pump control and is required for minimizing pressure fluctuations and optimizing flow and composition precision a
117. of the pump The amplitude of the pressure pulsation depends mainly on the stroke volume and the compressibility compensation for the solvent in use Small stroke volumes generate pressure pulsations of smaller amplitude than higher stroke volumes at the same flow rate In addition the frequency of the pressure pulsations is higher This decreases the influence of flow pulsations on quantitative results In gradient mode smaller stroke volumes result in a lower flow ripple improve composition ripple The module uses a processor controlled spindle system for driving its pistons The normal stroke volume is optimized for the selected flow rate Small flow rates use a small stroke volume while higher flow rates use a higher stroke volume By default the stroke volume for the pump is set to AUTO mode This means that the stroke is optimized for the flow rate in use A change to larger stroke volumes is possible but not recommended 1260 Binary Pump VL User Manual Introduction 1 Early Maintenance Feedback Maintenance requires the exchange of components which are subject to wear or stress Ideally the frequency at which components are exchanged should be based on the intensity of usage of the module and the analytical conditions and not on a predefined time interval The early maintenance feedback EMF feature monitors the usage of specific components in the instrument and provides feedback when the user selectable limits have been exceed
118. ons e anon instrument specific section called resident system e an instrument specific section called main system Resident System This resident section of the firmware is identical for all Agilent 1100 1200 1220 1260 1290 series modules Its properties are e the complete communication capabilities CAN LAN and RS 232C memory management e ability to update the firmware of the main system Main System Its properties are e the complete communication capabilities CAN LAN and RS 232C memory management e ability to update the firmware of the resident system In addition the main system comprises the instrument functions that are divided into common functions like e run synchronization through APG remote e error handling e diagnostic functions e or module specific functions like internal events such as lamp control filter movements raw data collection and conversion to absorbance 192 1260 Binary Pump VL User Manual Hardware Information 12 Firmware Updates Firmware updates can be done using your user interface e PC and Firmware Update Tool with local files on the hard disk e Instant Pilot G4208A with files from a USB Flash Disk e Agilent Lab Advisor software B 01 03 and above The file naming conventions are PPPP_RVVV_XXX dlb where PPPP is the product number for example 1315AB for the G1315A B DAD R the firmware revision for example A for G1315B or B for the G1315C DAD VVV is the revision nu
119. or pump head internal leaks Tools required p n Description 8710 2411 Hex key 3 mm12 cm long 8710 2392 Hex key 4 mm15 cm long T handle 01018 23702 Insert tool Screwdriver small flat head Parts required p n Description 0905 1175 Wash seal PTFE 5062 2484 Gasket seal wash pack of 6 5063 6586 Sapphire piston Preparations Switch off the pump at the main power switch Remove the front cover to have access to the pump mechanics Removing the Pump Head Assembly on page 135 Both pump head assemblies use the same internal components In addition pump head A is fitted with the purge valve The following procedure describes the removal and disassembly of pump head A left For pump head B right proceed in the same way and skip steps that deal with the purge valve 1260 Binary Pump VL User Manual 141 9 Maintenance 1 Place the pump head on a flat surface Loosen the lock screw two turns and while holding the lower half of the assembly piston housing carefully pull the pump housing away from the piston housing Lock screw Pump housin Piston housing 3 Check the piston surface and remove any deposits or layers Most suitable is polishing of the piston rod with toothpaste Replace the piston if scratched or if dents are visible Piston surface 2 Remove the seal holder and the seal wash support rings from the piston housing Remove the seal holder from the support ring assembly
120. ow to Prevent and or Reduce the Algae Problem 65 This chapter provides information for optimized usage of the binary pump gy Agilent Technologies 53 4 Using the Binary Pump Hints for Successful Use of the Binary Pump 54 Place solvent cabinet with the solvent bottles always on top or at a higher level of the pump When using the binary pump without vacuum degasser shortly degas your solvents by putting the solvent to a suitable container and applying a vacuum pressure for some time If possible apply solvent conditions that will decrease the gas solubility over time for example warming up the solvents The use of a vacuum degasser is mandatory for flow rates below 0 5 mL min and for configurations without damper and mixer When using the binary pump with vacuum degasser flush the degasser with at least 5 mL per channel before operating the pump especially when the pumping system had been turned off for a certain length of time for example overnight and volatile solvent mixtures are used in the channels see Regular Priming on page 50 Prevent blocking of solvent inlet filters never use the pump without solvent inlet filters Growth of algae should be avoided see Prevent Blocking of Solvent Filters and Algae Growth on page 64 Check purge valve frit and column frit in regular time intervals A blocked purge valve frit can be identified by black yellow or greenish layers on its surface or by a pressur
121. per Please contact your Agilent service representative 5 Defective main board Please contact your Agilent service representative 1260 Binary Pump VL User Manual 7 95 7 Error Information Pressure Signal Missing Error ID 2016 The pressure signal of the damper is missing The pressure signal of the damper must be within a specific voltage range If the pressure signal is missing the processor detects a voltage of approximately 120 mV across the damper connector Probable cause Suggested actions 1 Damper disconnected Please contact your Agilent service representative 2 Defective damper Please contact your Agilent service representative Valve Failed Error ID 2040 Valve 0 Failed valve A1 Valve 1 Failed valve A2 Valve 2 Failed valve B2 Valve 3 Failed valve B1 One of the solvent selection valves in the module failed to switch correctly The processor monitors the valve voltage before and after each switching cycle If the voltages are outside expected limits the error message is generated Probable cause Suggested actions 1 Solvent selection valve disconnected Please contact your Agilent service representative 2 Connection cable inside instrument not Please contact your Agilent service representative connected 3 Connection cable inside instrument Please contact your Agilent service representative defective 4 Solvent selection valve defective Exchange the solvent selection valve 96 1260 B
122. provides information on probable causes and suggested actions how to recover from error conditions 1260 Binary Pump VL User Manual Error Information 7 What are Error Messages Error messages are displayed in the user interface when an electronic mechanical or hydraulic flow path failure occurs which requires attention before the analysis can be continued for example repair or exchange of consumables is necessary In the event of such a failure the red status indicator at the front of the module is switched on and an entry is written into the module logbook 1260 Binary Pump VL User Manual 85 7 Error Information General Error Messages General error messages are generic to all Agilent series HPLC modules and may show up on other modules as well Timeout Error ID 0062 The timeout threshold was exceeded Probable cause Suggested actions 1 The analysis was completed successfully Check the logbook for the occurrence and and the timeout function switched off the source of a not ready condition Restart the module as requested analysis where required 2 Anot ready condition was present duringa Check the logbook for the occurrence and sequence or multiple injection run for a source of a not ready condition Restart the period longer than the timeout threshold analysis where required 86 1260 Binary Pump VL User Manual Shutdown Error ID 0063 An external instrument has generated a shutdown signal on the r
123. r 6 Tighten the screws of the valve holder 1260 Binary Pump VL User Manual 157 9 Maintenance Exchanging the Solvent Selection Valve 7 Reconnect all tubings to the solvent selection valve Ay J NOTE After an exchange of the valve it may be required to pump several mL of solvent before the flow stabilizes at a pressure ripple as low as it used to be when the system was still working properly 158 1260 Binary Pump VL User Manual Maintenance 9 Exchanging the Optional Interface Board When Board defective Parts required Description 1 BCD Interface board Preparations Switch OFF the module at the main power switch Unplug the module from main power CAUTION Electronic boards and components are sensitive to electrostatic discharge ESD ESD can damage electronic boards and components gt In order to prevent damage always use an ESD protection when handling electronic boards and components 1 Disconnect cables from the interface board connectors 2 Loosen the screws Slide out the interface board from the module BCD interface board Figure 16 Exchanging the Interface Board 3 Install the new interface board Secure the screws 4 Reconnect the cables to the board connector 1260 Binary Pump VL User Manual 159 9 Maintenance Replacing the Module Firmware When Tools required OR OR Parts required Preparations
124. r Manual 55 4 Using the Binary Pump Setting up the Agilent 1260 Infinity Binary Pump VL G1312C with the Instrument Control Interface Overview Parameters described in following sections is offered by the instrument control interface and can usually be accessed through Agilent instrument control software For details please refer to manuals and online help of respective user interfaces 56 1260 Binary Pump VL User Manual Using the Binary Pump 4 Setup of Basic Pump Parameters The most important parameters of the pump are listed in Table 5 on page 57 Table5 Basic pump parameters Parameter Limits Description Flow 0 001 5 mL min Total flow rate of the pump The optimum flow rate range is 0 1 to 5 mL min see Performance Specifications on page 24 Stop Time 0 01 min no limit The stop time of the pump usually controls the run time of the whole LC system which is the time during which data is acquired and saved to data files The stop time does not stop the pump flow etc Use no limit to stop the run manually useful for method development PostTime off 99999 min Time between the end of a run and the start of the next Used for column equilibration after a gradient Pressure Max 0 400 bar Max must be bigger than Min Set max pressure to the maximum operating Limits Min 0 400 bar pressure of your column A min pressure setting of e g 10 bar will turn off your pump automatically when running out o
125. r application and equilibrate the system for approx 10 minutes prior to starting a run 1260 Binary Pump VL User Manual 51 3 52 Installing the Pump Table 4 Choice of Priming Solvents for Different Purposes Activity Solvent Comments After an installation lsopropanol Best solvent to flush air out of When switching between lsopropanol the system reverse phase and normal Miscible with almost all phase both times solvents After an installation To clean the system when using buffers After changing aqueous solvents After the installation of normal phase seals PE seals pack of 2 p n 0905 1420 Ethanol or methanol HPLC grade water HPLC grade water Hexane 5 isopropanol Alternative to isopropanol second choice if no isopropanol is available Best solvent to re dissolve buffer crystals Best solvent to re dissolve buffer crystals Good wetting properties 1260 Binary Pump VL User Manual 1260 Binary Pump VL User Manual ee 4 Using the Binary Pump Hints for Successful Use of the Binary Pump 54 Setting up the Pump with the G4208A Instant Pilot 55 Setting up the Agilent 1260 Infinity Binary Pump VL G1312C with the Instrument Control Interface 56 Overview 56 Setup of Basic Pump Parameters 57 Pump Control 58 Auxiliary Pump Parameters 59 Data Curves 60 Bottle Filling 60 Solvent Information 62 Prevent Blocking of Solvent Filters and Algae Growth 64 Algae Growth in HPLC Systems 65 H
126. rew drives in opposite directions The gears for the ball screw drives have different circumferences ratio 2 1 allowing the first piston to move at double the speed of the second piston The solvent enters the pump heads close to the bottom limit and leaves it at its top The outer diameter of the piston is smaller than the inner diameter of the pump head chamber allowing the solvent to fill the gap in between The first piston has a stroke volume in the range of 20 uL to 100 uL depending on the flow rate The microprocessor controls all flow rates in a range of 1 uL min to 5 mL min The inlet of the first pumping unit is connected to the active inlet valve which is processor controlled opened or closed allowing solvent to be drawn into the first pump unit The outlet of the pump unit is connected directly to the second pump unit The outlet of the second pump unit is connected via a small mixing chamber a coil and the damping unit to the purge valve assembly The outlet of the purge valve assembly is then connected to the following chromatographic system When turned on the pump runs through an initialization procedure to determine the upper dead center of the first piston of both pump channels The first piston moves slowly upwards to the mechanical stop of the pump head and from there it moves back a predetermined path length The controller stores this piston position in memory After this initialization the pump starts operation with the set p
127. rly Maintenance Feedback 17 Instrument Layout 18 This chapter gives an introduction to the module instrument overview and internal connectors ee Agilent Technologies 1 Introduction Introduction to the Binary Pump Introduction to the Binary Pump Outlet valve Pump head A Purge valve Active inlet valve Mixing chamber Pump head B 10 The binary pump comprises two identical pumps integrated into one housing It provides gradient generation by high pressure mixing Degassing is not included but a vacuum degasser is available as a separate product for applications that require best flow stability especially at low flow rates or maximum detector sensitivity This is most likely required to run small internal diameter columns 2 mm and 1 mm i d which require low flow rates A solvent selection valve optional will allow to select a binary mixture isocratic and gradient from four independent solvent bottles An active seal wash optional is available when the pump is used with concentrated buffer solutions Figure 1 Overview of the binary pump 1260 Binary Pump VL User Manual Introduction 1 Overview of the Binary Pump The binary pump is based on a two channel dual piston in series design which comprises all essential functions that a solvent delivery system has to fulfill Metering of solvent and delivery to the high pressure side are performed by two pump assemblies which can generate pressure up to 400
128. se 0 Channels Al and A2 Valve Fuse 1 Channels B1 and B2 One of the solvent selection valves in the pump has drawn excessive current causing the selection valve electronic fuse to open Probable cause Suggested actions 1 Defective solvent selection valve Restart the capillary pump If the error message appears again exchange the solvent selection valve 2 Defective connection cable front panel to Please contact your Agilent service main board representative 3 Defective main board Please contact your Agilent service representative 4 1200 Series solvent selection valve installed Replace by 1260 solvent selection valve 1260 Binary Pump VL User Manual Error Information 7 AIV Fuse Error ID 2044 Inlet Valve Fuse 0 Pump channel A Inlet Valve Fuse 1 Pump channel B One of the active inlet valves in the module has drawn excessive current causing the inlet valve electronic fuse to open Probable cause Suggested actions 1 Defective active inlet valve Restart the module If the error message appears again exchange the active inlet valve 2 Defective connection cable front panel to Please contact your Agilent service main board representative 3 Defective main board Please contact your Agilent service representative Temperature Out of Range Error ID 2517 Temperature Out of Range 0 Pump channel A Temperature Out of Range 1 Pump channel B One of the temperature sensor readings in the motor drive circuit
129. sition for restarting When the module is switched on the first step is to switch on the C phase of the variable reluctance motor The rotor should move to one of the C positions The C position is required for the servo to be able to take control of the phase sequencing with the commutator If the rotor is unable to move or if the C position cannot be reached the error message is generated Probable cause 1 Disconnected or defective cable Blocked active inlet valve Mechanical blockage of the module Defective pump drive assembly Defective main board 1260 Binary Pump VL User Manual Suggested actions Please contact your Agilent service representative Exchange the active inlet valve Remove the pump head assembly Ensure there is no mechanical blockage of the pump head assembly or pump drive assembly Please contact your Agilent service representative Please contact your Agilent service representative 103 7 Error Information Pump Head Missing Error ID 2202 2212 Pump Head Missing Pump channel A B Pump Head Missing Pump channel B The pump head end stop in the pump was not found When the pump restarts the metering drive moves forward to the mechanical end stop Normally the end stop is reached within 20 s indicated by an increase in motor current If the end point is not found within 20 s the error message is generated Probable cause Suggested actions 1 Pump head not installed
130. status indicator indicates one of six possible module conditions When the status indicator is OFF and power switch light is on the module is in a prerun condition and is ready to begin an analysis A green status indicator indicates the module is performing an analysis run mode A yellow indicator indicates a not ready condition The module is ina not ready state when it is waiting for a specific condition to be reached or completed for example immediately after changing a set point or while a self test procedure is running An error condition is indicated when the status indicator is red An error condition indicates the module has detected an internal problem which affects correct operation of the module Usually an error condition requires attention e g leak defective internal components An error condition always interrupts the analysis If the error occurs during analysis it is propagated within the LC system i e ared LED may indicate a problem of a different module Use the status display of your user interface for finding the root cause module of the error A blinking indicator indicates that the module is in resident mode e g during update of main firmware A fast blinking indicator indicates that the module is in a low level error mode In such a case try to re boot the module or try a cold start see Special Settings on page 207 Then try a firmware update see Replacing the Module Firmware on p
131. t 1260 Infinity Binary Pump VL G1312C Type Specification Comments Composition range Composition precision Composition accuracy Control Local control Analog output Communications Safety and maintenance GLP features Housing settable range 0 100 recommended range 1 99 or 5 pL min per channel whatever is greater lt 0 15 RSD or lt 0 04 min SD whatever is greater at 0 2 and 1 mL min based on retention time at constant room temperature 0 5 absolute water caffeine tracer Agilent control software e g ChemStation EZChrom OL MassHunter Agilent Instant Pilot For pressure monitoring 2 mV bar one output Controller area network CAN RS 232C APG Remote ready start stop and shut down signals LAN optional Extensive diagnostics error detection and display through Agilent LabAdvisor leak detection safe leak handling leak output signal for shutdown of the pumping system Low voltage in major maintenance areas Early maintenance feedback EMF for continuous tracking of instrument usage in terms of seal wear and volume of pumped mobile phase with pre defined and user settable limits and feedback messages Electronic records of maintenance and errors All materials are recyclable For use with flow rates below 500 pL min a vacuum degasser is required 1260 Binary Pump VL User Manual 25 2 Site Requirements and Specifications Performance Specificat
132. t blocking 64 42 156 solvent zero counter 93 54 170 solvent inlet filter 54 solvent selection valve solvent cabinet solvent selection valve 10 solvents change 51 sound emission 216 special interfaces 203 special settings boot resident 207 forced cold start 207 specification performance 24 physical 23 specifications 19 stack configuration front view 34 rearview 35 static mixer 71 status indicator 79 stroke length 107 14 16 system setup and installation optimizing stack configuration stroke volume 1260 Binary Pump VL User Manual 31 Index T temperature limit exceeded 100 temperature out of range 99 temperature sensor 92 test functions 76 timeout 86 troubleshooting error messages status indicators 85 76 78 76 U unpacking the pump 28 V vacuum degasser when to use 68 vacuum degasser 25 vacuum degasser operational hints 68 vacuum degasser 10 54 54 valve failed 96 valve solvent selection 156 variable reluctance motor 14 variable stroke volume 16 voltage range 23 wW wait timeout 108 wash seals 130 wash solvent 69 waste electrical and electronic equipment 213 WEEE directive 213 weight 23 221 www agilent com In This Book This manual contains technical reference information about the Agilent 1260 Infinity Binary Pump VL G1312C The manual describes the following introduction requirements e installation configuring the pump e usi
133. terface board Analog output o iC ic CAN Bus Power plug Security lever Figure 27 Electrical connections to the binary pump NOTE The GPIB interface has been removed with the introduction of the 1260 Infinity modules 196 1260 Binary Pump VL User Manual Hardware Information 12 Interfaces The Agilent 1200 Infinity Series modules provide the following interfaces Table 11 Agilent 1200 Infinity Series Interfaces Module CAN LAN BCD LAN RS 232 Analog APG Special optional on board Remote Pumps G1310B Iso Pump 2 Yes No Yes 1 Yes G1311B Quat Pump G1311C Quat Pump VL G1312B Bin Pump G1312C Bin Pump VL 1376A Cap Pump G2226A Nano Pump G5611A Bio inert Quat Pump G4220A B Bin Pump 2 No Yes Yes No Yes G1361A Prep Pump 2 Yes No Yes No Yes CAN DC OUT for CAN slaves Samplers G1329B ALS 2 Yes No Yes No Yes THERMOSTAT for G2260A Prep ALS G1330B G1364B FC PS 2 Yes No Yes No Yes THERMOSTAT for G1364C FC AS G1330B G1364D FC uS CAN DC OUT for CAN G1367E HiP ALS slaves G1377A HiP micro ALS G2258A DL ALS G5664A Bio inert FC AS G5667A Bio inert Autosampler G4226A ALS 2 Yes No Yes No Yes 1260 Binary Pump VL User Manual 197 12 Hardware Information Table 11 Agilent 1200 Infinity Series Interfaces Module CAN LAN BCD LAN RS 232 Analog APG Special optional on
134. the Blank Nut To test the complete system s pressure tightness the blank nut should be positioned at the column compartment outlet or the outlet of the last module before the detector If a specific component is suspected of causing a system leak place the blank nut immediately before the suspected component then run the Pressure Test again If the test passes the defective component is located after the blank nut Confirm the diagnosis by placing the blank nut immediately after the suspected component The diagnosis is confirmed if the test fails 1260 Binary Pump VL User Manual 113 8 Test Functions and Calibration Running the Pressure Test When Tools required Parts required Preparations 114 If problems with small leaks are suspected After maintenance of flow path components e g pump seals injection seal to prove pressure tightness up to 400 bar Description Wrench 1 4 inch p n Description 1 01080 83202 Blank nut 1 500 mL Isopropanol Place a bottle of LC grade isopropanol in the solvent cabinet and connect it to channel A or channel A2 if solvent selection valve is installed Make absolutely sure that all parts of the flow path that are part of the test are flushed very thoroughly with isopropanol before starting to pressurize the system Any trace of other solvents or the smallest air bubble inside the flow path definitely will cause the test to fail Running the test from the Agilent Lab Monitor
135. the purge valve when the test has finished Evaluating the Results on page 120 describes the evaluation and interpretation of the leak test results Detailed instructions are provided in the Lab Advisor Software 1260 Binary Pump VL User Manual 119 8 Test Functions and Calibration Evaluating the Results Defective or leaky components in the pump head lead to changes in the Leak Test pressure plot Typical failure modes are described below Please notice the difference between an error in the test and a failure of the test An error means that during the operation of the test there was an abnormal termination If a test failed this means that the results of the test were not within the specified limits Often it is only the damaged blank nut itself poorly shaped from overtightening that causes a failure of the test Before investigating on any other possible sources of failure make sure that the blank nut you are using is in good condition and properly tightened No pressure increase or minimum pressure of plateau 1 not reached Probable cause Suggested actions 1 Pump not running Check the logbook for error messages 2 Purge valve open Close the purge valve and restart the test 3 Wrong solvent line connections to solvent Ensure the solvent lines from the degasser to selection valve the solvent selection valve are connected correctly 4 Loose or leaky fittings Ensure all fittings are tight or exchange capillary 5 L
136. ut the damage gt An Agilent service representative will inspect the instrument at your site and initiate appropriate actions 28 1260 Binary Pump VL User Manual Delivery Checklist Installing the Pump 3 Ensure all parts and materials have been delivered with the pump For checking the completeness of your specific shipment please use the list included in your shipment To aid in parts identification please refer to Parts for Maintenance on page 161 Please report missing or damaged parts to your local Agilent Technologies sales and service office p n G1312C G4203 68708 G4201 68707 G4202 68707 G1369C G4800 64500 G1311 60003 2x 959961 902 699975 902 883975 902 61312 90302 5067 4770 M8500A Description Agilent 1260 Infinity Binary Pump VL HPLC System Tool Kit OPTIONAL HPLC Starter Kit incl 0 17 mm i d cap OPTIONAL HPLC Starter Kit incl 0 12 mm i d cap OPTIONAL Interface board LAN OPTIONAL Agilent 1200 Infinity Series User Documentation DVD OPTIONAL not orderable OPTIONAL Bottle head assembly Column Eclipse Plus C18 4 6 x 100 mm 3 5 pm OPTIONAL Column Poroshell 120 EC C18 4 6 x 50 mm 2 7 um OPTIONAL Column SB C18 4 6 x 150 mm 5 pm OPTIONAL Agilent 1260 Infinity Binary LC Optimization Guide not orderable Solvent Cabinet Kit OPTIONAL Lab Advisor incl license OPTIONAL Power cord Items identified as optional are additional accessories They are not included
137. ve Power Pump channel B The current drawn by the pump motor exceeded the maximum limit Blockages in the flow path are usually detected by the pressure sensor in the damper which result in the pump switching off when the upper pressure limit is exceeded If a blockage occurs before the damper the pressure increase cannot be detected by the pressure sensor and the module will continue to pump As pressure increases the pump drive draws more current When the current reaches the maximum limit the module is switched off and the error message is generated Probable cause Suggested actions 1 Flow path blockage in front of the damper Ensure the capillaries and frits between the pump head and damper inlet are free from blockage 2 Blocked active inlet valve Exchange the active inlet valve 3 Blocked outlet ball valve Exchange the outlet ball valve 4 High friction partial mechanical blockage in Remove the pump head assembly Ensure there the pump drive assembly is no mechanical blockage of the pump head assembly or pump drive assembly 5 Defective pump drive assembly Please contact your Agilent service representative 6 Defective main board Please contact your Agilent service representative 7 Restriction capillary blocked at pre mixing Exchange restriction capillary union 1260 Binary Pump VL User Manual 101 7 102 Error Information Encoder Missing Error ID 2046 2050 2510 Encoder Missing Pump channel A B En
138. ve in channel A 5 Leaking seal or scratched piston in channel Al 6 Defective active inlet valve in channel A Suggested actions Flush channel A thoroughly with isopropanol under pressure use restriction capillary Tighten the active inlet valve in channel A 14 mm wrench Do not overtighten Ensure the pump head screws in channel A are tight Ensure the sieve in the outlet valve is installed correctly Tighten the outlet valve Exchange the pump seals in channel A Check the pistons for scratches Exchange if scratched Exchange the active inlet valve in channel A Fourth plateau negative or unstable and at least one other plateau positive Probable cause 1 Airin pump chamber of channel B or seals not yet seated 2 Loose active inlet valve in channel B 3 Loose pump head screws in channel B 4 Loose outlet valve in channel B 5 Leaking seal or scratched piston in channel B1 6 Defective active inlet valve in channel B 1260 Binary Pump VL User Manual Suggested actions Flush channel B thoroughly with isopropanol under pressure restriction capillary Tighten the active inlet valve in channel B 14mm wrench Do not overtighten Ensure the pump head screws in channel B are tight Ensure the sieve in the outlet valve is installed correctly Tighten the outlet valve Exchange the pump seals in channel B Check the pistons for scratches Exchange if scratched Exchange the active inlet

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