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Manual - ProgRes® MAC CapturePro 2.7

1. ProgRes CCD routine cameras Calibration pattekty EDO iier ProgRes MAC CapturePro 2 7 Release 001 Contents Introduction 1 Introduction 151 Welcome Welcome to your new Jenoptik ProgRes digital camera system You have purchased a reliable state of the art digital microscope camera for image analysis and documentation with the operational software ProgRes MAC CapturePro for operating your microscope camera from a Macintosh computer Fig 1 1 Microscope Workstation with ProgRes camera The following manual applies to the operation of the ProgRes microscope cameras using the ProgRes MAC CapturePro application software In case that any differences occur in operating the camera types you will be explicitly informed about these differences Operating your ProgRes camera with the new ProgRes MAC Capture Pro 2 7 application software is easy and intuitive Nevertheless please ProgRes MAC CapturePro 2 7 Release 001 7 Introduction take the time to read through this operating manual in order to get familiar with the camera the software and the safety advice Important note Please take the time to register as a ProgRes user on our web site a www progres camera As a registered user you will have access to software downloads and you will be automatically informed when a software up
2. 19 3 3 ounting the ProgRes camera to a microscope wld 3 4 Connecting the ProgRes camera to a 19 4 Software user interface 21 5 gom 23 5 1 The menu ProgRes9 ciecen naana 23 5 2 Themen Fle sia secos Ret 24 5 3 Testen iade 25 5 4 The menu Camera ertet nn 25 5 5 The menu 25 6 mage display and acquisition 26 6 1 Acqire mode Live image display 26 6 2 Display saved images in a gallery 27 6 2 1 Load an image from the 28 6 2 2 Edit an image in the gallery 6 3 Teicon en 6 3 1 Display exposure 95 6 3 2 ZOOM etate ProgRes MAC CapturePro 2 7 Release 001 4 6 3 3 6 3 4 6 3 5 6 3 6 6 3 7 M 1 2 1 1 2 2 1 2 3 1 2 4 1 4 1 1 4 2 1 4 3 NNNNNNNNNNNNNN SON N Oo 7 3 7 3 1 7 3 2 7 3 3 7 3 4 7 4 7 4 1 7 4 2 7 5 7 5 1 7 5 2 7 5 3 8 8 1 8 2 8 3 8 4 Setting and saving image crops 30 White balance 30 Black balalfice et e etes 31 Change color Mode
3. Color Use Font Color 7 Line width 1 c Font Color Use Line Color Font Courier New 12 Set Font Scale fa Subdivisons 20 Appearance V Show Frame C Label Show Length Images M Save Grayscale Images as Color Images Reset to Factory Settings GOD Fig 7 10 Index card Measurement n the index card Measurement the settings for the display of the measurement overlay in the image can be defined Lines The color and the width of the lines can be set A line width of one pixel is set by default The line width can be altered using the arrow buttons The line color can be selected with a click on the color box A dialogue opens where the color can be selected When the button Use Font Color is pressed the color selected for the font will be applied to the lines Font The font color can be defined with a click on the color box and by selecting the color in the subsequently opened window The font can be set by a click on the button Set Font and by defining the font parameters in the subsequently opened window ProgRes MAC CapturePro 2 7 Release 001 42 Operational windows Setting preferences Colors 000 Font 9 ut a Em iaa 9 315 Collections Family Typeface Size All Fonts Brush Script MT Regular 12 Favorites Chalkboard 0 Italic 9 m Rece
4. E Fig 6 2 Acquire screen After connecting your camera the live image from the microscope is displayed in the software user interface The image is continuously refreshed and will not be stored on your hard disk drive unless captured with a click on the button Acquire You can either use the button Acquire above of the operating panel or in the lower right corner marked with a red circle If the image exceeds the size of the image window you can use the scroll bars below and at the right side for moving the image If no camera is connected NOT CONNECTED will be displayed in the ProgRes MAC CapturePro 2 7 Release 001 26 Image display and acquisition image window Settings for the camera and the image adjustments can be selected The operation panel left of the main image display window offers func tions for the definition of the following settings Image resolution for live mode capture mode exposure and gain e Contrast brightness gamma and saturation e Color balance adjustment detailed description of the operating panel and its functions and the details of the resolution of the different camera types can be found in chapter 8 Operating panel 07050 005048 079202 00009 070502 0078 image 070502 0060 070002 0033 070812 001848 o 25 KL 415 Fig 6 3 Im
5. a user interface The function is opened by right clicking 50 0 onto the Zoom buttons 75 0 100 0 v 200 0 DE In the upper area the zoom factor percentage can be 0 selected 500 0 600 0 5 The Zoom to fit function adapts the image automatical Zoomtort ly to the software user interface Zoom In Zoom Out ProgRes MAC CapturePro 2 7 Release 001 29 6 3 3 E 6 3 4 Image display and acquisition The Hand tool can be used to navigate within an enlar ged image Click left into the image and pull the mouse into the desired direction The image region in the display window will be shifted accordingly Using the Rotate button the image can be rotated in steps of 90 Setting and saving image crops Using the button Crop an image region can be defined that can be saved A rectangle appears for the selection of this image area The selected crop region can be saved with a click on the button Acquire The crop images can be displayed and loaded in a gallery Using crop images saving time and storage capacity can be reduced The dimensions of this area can be changed with a click on the corners of the rectangle and by moving the mouse simultaneously The position of the rectangle is changed by a click into the rectangle and by moving the mouse When the button Crop is clicked again the rectangle disappears With a repeated click on the Crop button
6. 61 Operating b onte iier 62 Adjust brightness contrast gamma and saturation 62 Adjust balance ss 63 Image resolution sinceiro ipni 64 Adjustment of exposure and 64 ProgRes MAC CapturePro 2 7 Release 001 Contents 8 4 1 8 4 2 8 5 10 11 11 1 11 2 11 3 11 4 12 12 1 12 1 1 121 2 12 1 3 12 1 4 12 2 12 3 12 3 1 12 3 2 12 3 3 13 Adjust expos fe etnies ne tee diced ten 64 Adjust hepate 65 gister 67 mage description area 68 mage information bar eet ee tees 69 Service and support sssssssssseeeeeees 70 anufacturer service amp 70 Return address for goods in case of service incidents 70 Camera registration and software updates Further Information eessseesensensenennennennenen Technical specifications of ProgRes cameras General Type plate and order numbers Scope of delivery sss Certificates and approvements System requirements essentiae Image resolution of the ProgRes9 cameras Technical data sheets ProgRes CCD research cameras ProgRes CMOS
7. 6 3 7 Measurement layer A measurement layer comprises one or more shapes which can be inserted into the image P With a click on this button the measurement layer can be shown or hidden To select a measurement layer option click on the arrow buttons next A drop down menu opens where the mea surement shape can be selected ProgRes MAC CapturePro 2 7 Release 001 31 Image display and acquisition Aline or a horizontal vertical scale can be inserted into the image The size of these shapes can be changed with a click on the dots at the corners of the shapes To change the size of the measurement shapes retroactively click on the shape and pull the corner dots with the mouse When the button Acquire is clicked the layer will be saved with the image The layer of a saved image cannot be edited retroactively When the layer is hidden it will not be saved with the image ProgRes MAC CapturePro 2 7 Release 001 32 2 Operational windows Operational windows non uon non The buttons Preferences Timelapse Calibration Measure and Fluorescence enable the access to further operations of the software By clicking the buttons a window opens for defining settings or perfor ming image capture functions The buttons are located in the upper right area above of the image display and enable the following functions 8 e Preferences Set preferences for image capturing and saving The prefere
8. Licensor Summary JENOPTIk Laser Optik Systeme GmbH G schwitzer Str 25 D 07745 Jena Germany This End User License Agreement the License Agreement constitutes a legally binding contract between you if an individual or a corporate entity the Licensee and JENOPTIK Laser Optik Systeme GmbH the Licensor You are requested to carefully read the provisions of this License Agreement before you start using the Product as defined in 5 1 Tt amp below The Product includes software made available to you by JENOPTIK Y ProgRes gt gt mm Fig 3 2 End user license agreement n the next screen the end user license is displayed The license must be accepted When Continue is clicked a dialogue window is displayed where the end user license can be accepted Standard Install on Macintosh HD Introduction Read Me This will take 12 0 KB of space on your computer License Click Install to perform a standard installation of Destination Select this software on the volume Macintosh HD Installation Type Installation Summary ProgRes 4 Fig 3 3 Select destination n this screen the installation location can be selected If you click Change Install Location a window opens where the installation loca tion can be selected If no installation location is selected the program is installed automatically in
9. 13 01 Q O50 ProgRes Mac CapturePro 26 119 LJ CNewSersion 29151913 ass Fig 7 24 Fluorescence image with gallery ProgRes MAC CapturePro 2 7 Release 001 59 Operational windows Fluorescence Adjust color intensity and merge images Gallery Color intensity adjustment and image merging can be carried out in the area Show Captured Monochrom Images Colored Gallery O Merged The captured images can be displayed Image _ Merge Intensity monochrome i e in black and white Fitc mode in color i e in the respective fil ter color or merged When Merged is activated the area Pixelshift becomes active too Reset Intensity Fig 7 25 Gallery options For a multi channel fluorescence image the individual images included in the merged image can be selected by marking the checkbox under Merge With the slider control bars the intensity of each image can be changed If you are not pleased with the adjustments click the button Reset intensity Pixelshifting Reset intensity When Merge is activated in the area Pixelshift Show captured images the area 210 Di fra A Pixelshift becomes active 4x Here the displacements of the superim Tritc v posed individual fluorescence images
10. 31 Measurement layer e 31 Operational windows 33 Setting preferences au 34 Image IO thi E 34 ihnen 35 Setting prefix and 35 be estie Auto Open Oi d User interface BIET 40 SAVE IMAGES AS uiis rede tovto E a een 40 POLO CO 40 COMMO ete itera de iin 41 tereti eter ira tete uten 42 5 6 gettin n aee get 44 Timelapse time controlled image capturing 45 448 EE Black shading White Shading Calibration Scanner calibration Activate dte tc dee teen dresden easurement window Create or edit a calibration aes nsert a new unit or edit a unit Fluorescence image capturing Carry out a fluorescence Adjust captured fluorescence 59 Load fluorescence projects
11. 800 x 600 SVGA 640 x 480 VGA 680 x 512 2x Bin 349 x 256 4x Bin 170 x 128 8x Bin ProgRes MAC CapturePro 2 7 Release 001 Technical specifications 77 ProgRes MF USB Resolution Capture 1360 x 1024 1280 x 1024 SXGA 1024 x 768 XGA 800 x 600 SVGA 640 x 480 VGA 680 x 512 2x Bin 349 x 256 4 Bin 170 128 8x Bin x x DX ProgRes CT1 C USB Resolution Capture 1280 x 1024 1024 x 768 XGA 800 x 600 SVGA 640 x 480 VGA ProgRes CT1 M USB Resolution Capture 1280 x 1024 1024 x 768 XGA 800 x 600 SVGA 640 x 480 VGA ProgRes USB Resolution Live Capture 2048 x 1536 1600 x 1200 UXGA 1280 x 1024 SXGA 1024 x 768 XGA x x ProgRes MAC CapturePro 2 7 Release 0 0 Technical specifications 78 800 600 SVGA 640 480 1024 768 2x Bin 682 x 512 3x Bin x lt ProgRes CT5 C USB Resolution Live Capture 2592 x 1944 2048 x 1536 QXGA 1600 x 1200 UXGA 1280 x 1024 SXGA 1024 x 768 XGA 800 x 600 SVGA 640 x 480 VGA 1296 x 972 2x Bin 648 x
12. tion once a month Er Please note The deterioration of the image quality due to incorrect scanner calibration can be recognized at the horizontal or vertical smooth edges in the image Transitions between black and white at a high image resolution gt 200 are especially suitable to check the scanner calibration if these edges appear jagged the scanner calibration may be incorrect How to carry out a calibration To carry out a successful calibration of the camera a pattern with sharp light and dark contrasts within a measurement section is necessary calibration patterns are included in delivery with the ProgRes C14 s ProgRes CFs and ProgRes For a calibration using a micro scope an object carrier with two different gratings is included in your delivery For the calibration of a C mount lens a special calibration pattern is included in the appendix of this manual Setting up the calibration pattern ProgRes File Edit Camera Window 4 cei oos Fig 7 18 Image display during camera calibration with calibration rectangle ProgRes MAC CapturePro 2 7 Release 001 51 Operational windows Calibration The following criteria have to be considered Position The calibration pattern has to be set up parallel to the image capture plane i e perpendicular to the optical axis This is always the case when a microscope is used The orientation of the pattern is not important for the calibratio
13. OK the changes inserted in the window are applied With Cancel the application is reset to the action last performed PAA Image info image Info Acquire User interface Timelapse Measurement Fluorescence Save Images in Directory Users G4Lapi Pictures Images Capture Pro Capture_Test 16 04 2007_15 54 51 Choose Folder Default Folder Show Folder Save Images as Single images ee 6 QuickTime Movie Setup m ing Save Protocol f9 Auto Save O Never O Ask user Time Control Use Minimal Possible Interval Use Time Settings Total Time and Interval Time in msec Interval Time 00 00 00 500 2 Images 10 ga C cancer EB Fig 7 2 Dialogue Preferences Image Info ProgRes MAC CapturePro 2 7 Release 001 34 Operational windows Setting preferences Information about the author the user and the company and an image description can be entered into the input boxes that will be saved as a meta file with the captured image By marking the respective checkbox the image information to be saved with the captured image can be selected The detailed comment will be displayed in the description box under the image in the Acquire mode Note The saving of the image information reduces the cap ture performance in time lapse capture Important note The image information can only be stored with the file formats TIFF Photos
14. 204 5x Bin x 1 x x lt x ProgRes MAC CapturePro 2 7 Release 001 75 ProgRes MF MF Resolution Live Capture 1360 x 1024 680 x 512 HFRM 340 x 256 HFRM 680 x 512 2x Bin 452 x 340 3x Bin 340 x 256 4x Bin 272 x 204 5x Bin x x x ProgRes Resolution Live Capture 1360 x 1024 680 x 512 HFRM 340 x 256 HFRM 4080 x 3072 9x Scan 2720 x 2048 4x Scan x 680 x 512 2x Bin 452 x 340 3x Bin 340 x 256 4x Bin 272 x 204 5x Bin x x ProgRes C14 Plus Resolution Live Capture 1360 x 1024 HQ 1360 x 1024 680 x 512 680 x 512 HFRM x 340 x 256 HFRM 4080 x 3072 36x Scan ProgRes MAC CapturePro 2 7 Release 0 0 Technical specifications 76 4080 3072 9 Scan 2720 2048 16x Scan 1360 1024 4 Scan 452 x 340 3x Bin 272 x 204 5x Bin ProgRes CS USB Resolution Live Capture 782 x 582 640 x 480 VGA ProgRes MS USB Resolution Live Capture 782 x 582 x 640 x 480 VGA x ProgRes CF USB Resolution Live Capture 1360 x 1024 1280 x 1024 SXGA 1024 x 768 XGA
15. Color E 35 Threshold 255 V Underexposure Color Threshold 10 Reset to Factory Settings Fig 7 6 Dialogue User interface with color selection box n the dialogue User interface the display of over or underexposed areas in the image can be selected ark the checkbox to activate the display of the exposure warning Click the arrow buttons to adjust the theshold value for the over or un derexposed areas It is recommended to set a value of 250 grey values for overexposure and of 5 grey values for underexposure To select the color for the under and overexposed areas click on the colored boxes A dialogue opens where the color for the display of exposure warnings can be selected The colored areas in the image will indicate under or over exposed areas ProgRes MAC CapturePro 2 7 Release 001 38 Operational windows Setting preferences By a click on the button in the icon bar the under or overexposed areas in the image selected in the register card are shown or hidden With Reset to Factory Settings the settings in the index card User interface are reset to the factory settings With the changes inserted in the window will be applied Click Cancel to reset the changes to the factory settings 7 1 4 Timelapse ImageInfo Acquire User Interface Timeikapse Measurement Fluorescence Save Images in Di
16. Prefix example_image Next Image example_image00002 jpg Directory Users G4Lapi Deskt 13 04 2007 12 10 35 Description Acquire Fig 9 1 Image description area n the area Description an image description can be entered In the input box Prefix an image prefix can be inserted When images are captured a current number is added to the prefix for every image Under the Prefix field the name of the subsequently captured image and the directory where the captured images are stored are listed When the Acquire button is pressed the image is captured according to the settings specified under Preferences gt chapter 7 1 Setting Preferences The Acquire button changes its shape and color during image capturing ProgRes MAC CapturePro 2 7 Release 001 68 Image information bar 10 Image information bar 100 x ve Video 171 fr Zoom Proghes Ci4plus 0306 20 0120 ET RS ED Fig 10 1 Information bar n the image information bar the following information are displayed from left to right Abbreviation Information displayed Live video Frame rate fps frames per second Zoom Zoom factor applied to the image Coordinates of the cursor position in the image R G B RGB values of the current cursor position Camera name and serial number of the currently active camera With a click on the blue arrow button the cameras connected are displayed in a
17. camera and are listed below Camera type Analog gain max Gain max ProgRes C3 18 ProgRes C5 18 ProgRes C7 16 16 achieved via ana log gain ProgRes CT3 s 3 ProgRes C145 8 60 ProgRes MF 8 18 ProgRes 8 30 ProgRes MP 8 30 ProgRes CF 8 18 ProgRes 8 30 ProgRes CFS 8 30 ProgRes CS USB 8 ProgRes MS USB 8 ProgRes MAC CapturePro 2 7 Release 001 66 Operating panel ProgRes CF USB ProgRes MF USB ProgRes C USB ProgRes CT1 M USB ProgRes CT3 USB ProgRes 5 CUSB ProgRes 5 M USB 8 5 Histogram The histogram shows an overview over the distribution of tonal values in the image key type In an image with low color values low key the details are mainly in the depths In an image with high color values high key the details are in the lights Middle color values average key indicate the middle tones An image with a complete tonal value range has a certain number of pixels in any region Using the sliders under the histogram the tonal values can be corrected By moving the sliders the area can be defined in which the tone values can be adjusted Fig 8 3 Histogram ProgRes MAC CapturePro 2 7 Release 001 67 Image description area 9 Image description area Example description
18. can be added with a click on 171 A window opens where the name of the unit and the length of the unit can be inserted in mm Click to apply the settings New Unit Name New Unit Length in Millimeters 1 000000 Gree EB Fig 7 22 Insert new unit With a click on a selected unit can be removed When the unit is selected and Edit is clicked the unit can be edited Note Only the units that have been individually defined by the user can be edited or removed The units which have been set by default cannot be changed by the user When a scale is inserted into the image the length of the scale will be shown in the box in the area Scale using the selected unit If several scales have been inserted into the image a particular scale can be selected ProgRes MAC CapturePro 2 7 Release 001 56 Operational windows Fluorescence 75 Fluorescence image capturing With a click on the button lt a window opens offering functions for fluorescence measurement E With the button New Session a new fluore scence session is started With the button Last Session the fluorescence project which was last created is opened me In the field Acquire functions for fluorescence Dim image acquisition can be selected When the checkbox Apply Filter Color is marked the fluorescence image is displayed in the respective
19. can be adjusted Save Merged Image Fig 7 26 Area Pixelshift in the drawer Fluorescence ark the respective image in the gallery The name of the image is displayed in the area Pixelshift here TRITC Use the arrow buttons to shift the image By a click on the cross in the middle the last performed step of the pixelshifting can be reset With a click on Reset Pixelshift the pixel shift applied to the image in this session can be reset ProgRes MAC CapturePro 2 7 Release 001 60 Operational windows Fluorescence When Save Merged Image is clicked the merged image is saved and added to the fluorescence project It is added to the gallery and will be available when the fluorescence project is saved and loaded 153 Load fluorescence projects With a click on the button Last Session in the window Fluore scence the last fluorescence project can be loaded To load another fluorescence project click the button Gallery in the icon bar A dialogue opens where the folder can be selected ProgRes MAC CapturePro 2 7 Release 001 61 Operating panel 8 Operating Panel 100 4 In the operating panel the image settings ne be selected Camm Qn 050 Saturation M 000 s 4 In the Acquire and in the Gallery mode meee S the contrast brightness gamma and satura we 0 S can be set Also the color balan
20. filter color that has been preset in the window wu Preferences otherwise it is displayed in black and a white mode 50 x Trin v The field Gallery becomes active after the com pletion of the fluorescence project and enables the adjustment of the color display and the inten sity as well as the merging of images Fig 7 23 Fluorescence mode n the area Pixelshift the superimposed individual images can be ad justed Displacement due incorrect alignment of the fluorescence filters can be compensated By a click on the button Save merged image the merged image can be saved and will be displayed in the gallery ProgRes MAC CapturePro 2 7 Release 001 57 Operational windows Fluorescence 7 3 1 Carry out a fluorescence project Set fluorescence filters Before starting the fluorescence project insert the names of the filters in the index card Fluorescence under Preferences gt chapter 7 5 Fluorescence image capturing In the window Fluorescence mark the filters you will use in the checkbox Active Adjust the fluorescence slide preparation under the microscope and set the correct image focus Start session Click the button Start session A dialogue appears requesting the activation of the first fluorescence filter on your microscope Unless the checkbox Apply Filter Color is marked the fluorescence image will be displayed in black and white mode Adjust th
21. pop up window The currently active camera is marked Up to five cameras can be connected simultaneously ProgRes MAC CapturePro 2 7 Release 001 69 Service and support 11 Service and support Your first contact partner for questions about ProgRes microscope ca meras is your local expert dealer from whom you have purchased your ProgRes camera He is trained in using the camera and can answer your questions He can also advise you in using accessories for micro scope image capturing and in applications of the microscope camera 11 1 Manufacturer service amp support Furthermore our local technical expert support in Jena is at your ser vice Please contact Stefan Seidlein jenoptik com 11 2 Return address for goods in case of service incidents JENOPTIK Optical Systems Business Unit Digital Imaging Wareneingang Pruessingstra e 41 D 07745 Jena Germany 11 3 Camera registration and software updates You are free to register at our web site www progres camera com ps user of a ProgRes microscope camera As a registered user you are entitled to access the download area of our ProgRes web site where software updates are provided regularly free of charge You will be notified automatically if new software updates are available 11 4 Further Information More information about digital imaging products from JENOPTIK are available at ww progres camera com ww jenoptik los com ProgRes MAC C
22. supplied additionally using the suitable repeater 7 Suitable PCIMCIA cards and Firewire boards are listed in chapter 12 Technical specifications ProgRes MAC CapturePro 2 7 Release 001 20 Software user interface 4 Software user interface Fig 4 1 Software user interface The software user interface is divided into the following areas Menu bar orange frame In the menu bar the features and functions for the general operation of the ProgRes MAC CapturePro software are found chapter Menu bar Buttons Acquire and Gallery red frame The main section buttons Acquire and Gallery enable image acqui sition and the display of saved images in a gallery chapter 6 Image isplay and acquisition Image window yellow frame In the image window the live image or the images loaded from the gallery are displayed ProgRes MAC CapturePro 2 7 Release 001 21 Software user interface Icon bar green frame The buttons in the icon bar enable the access to several image settings gt chapter 6 3 The icon bar Buttons for operational windows brown frame By clicking these buttons operational windows are opened on the right side oft the software interface enabling the setting of preferences the time set image capturing shading calibration measurement calibration and fluorescence image capturing Operating panel blue frame The operating panel enables functions fo
23. the directory Applications By clicking ProgRes MAC CapturePro 2 7 Release 001 17 Installation and software start Continue the installation will be continued Date Modified Sue Kind 2 untied 74 License nf 2 April 2008 16 23 132K8 Rich T RTF B Macintosh gt Manual Today 13 31 Folder C ProgRes Mac CapturePro Today 13 31 149MB Application 9 ProgRes Mac ReadMe rtf 10 July 2008 11 20 Rich T RTF a Drop Projekte Y SEARCH FOR Today Yesterday O Past Week BAI images Gi Al Movies BAN Documents i velle Galerien Pr sentationen i meine Word Doku Fig 3 4 Display folders The application software is installed The folder Progres Mac Capture Pro is created and the files contained in this folder are displayed in the screen After installing the application software the ProgRes camera can be connected to your computer by the supplied FireWire cable s Note It is recommended to deactivate the Power Save settings of your computer while working with the ProgRes camera ProgRes MAC CapturePro 2 7 Release 001 18 Installation and software start 3 2 Starting the ProgRes MAC CapturePro Connect your ProgRes camera to your computer with the supplied FireWire cable You can operate ProgRes cameras that are equipped by a Firewire interface using the ProgRes MAC CapturePro software 5 Start ProgRes MA
24. 0 2 8 Images 10 8 Fig 7 9 Preferences Time control settings Here the settings for the time controlled image capturing are defined The image capturing can be performed either by using the minimal possible interval or by using the time settings which are inserted by the user Depending on the selected option the input boxes Total time and Interval will be active or will change automatically Use Minimal Possible Interval When this option is selected the images will be captured as fast as possible The image number has to be inserted into the box Images The input boxes Total Time or Interval Time will be deactivated Use Time Settings Here the total recording time Total Time the time interval between two captured images Interval Time and the total number of images can be inserted By clicking on the padlock symbol one option can be set as fixed The data of the other options will be adjusted automatical ly if one of them is changed Example A fixed time interval between two captured images is set which cannot be altered When the number of images is changed the total recording time is adapted automatically ProgRes MAC CapturePro 2 7 Release 001 41 Operational windows Setting preferences r ProgRes Mac Capture Pro 2 5 4 Image Info Acquire User interface Time Lapse Measurement Fluorescence Lines
25. 486 4x Bin x x x X ProgRes CT5 M USB Resolution Capture 2592 x 1944 2048 x 1536 QXGA 1600 x 1200 UXGA 1280 x 1024 SXGA 1024 x 768 XGA 800 x 600 SVGA 640 x 480 VGA 1296 x 972 2x Bin 648 x 486 4x Bin x ProgRes MAC CapturePro 2 7 Release 001 Technical specifications 79 Technical specifications 12 3 Technical data sheets 12 3 1 ProgRes CCD research cameras ProgRes CS MS ProgRes CF MF 7 Please note ProgRes USB cameras are presently not supported by ProgRes MAC CapturePro ProgRes camera type CS 5 CF MF Image sensor 1 2 CCD progress 2 3 CCD progress scan scan Color Monochrome Color Monochrome Color Monochrome Sensor resolution 782 x 582 pixel 1360 x 1024 pixel max 0 45 Mpix 1 4 Mpix Pixel size 8 3 um 6 45 um A D conversion 12 Bit 12 Bit Pixel clock 29 5 MHz 12 MHz 24 5 MHz Dynamic range 68 dB 65 67 dB Exposure times 50 us 2705 200 us 1805 Analog gain 1 8X 1X 8X Max frame rate 50 fps 782 x 582 13 fps 1360 x 1024 image size pixel pixel mage resolution Binning no 2x 5X Microscan no no True color no no Cooling no no Digital interface USB 2 0 USB 2 0 Firewire a Optical connection C Mount 0 5x TV pref Synchronization with external devices confi gurable via co
26. 69 dB 67 69 dB 200 us 600 s 200 us 300 5 1X 8x 1X ace BX 13 fps 1360 x 1024 13 fps 1360 x 1024 pixel pixel 2x 5X 2X 5X 2 7 Release 001 81 ProgRes camera type Microscan True color Cooling Digital interface Optical connection Trigger In Out Voltage supply Power consumption Ambient conditions Stock conditions Dimensions L W x H Weight Capture software External camera driver Hardware requirements Technical specifications CFeool M Feo CFsean C1 Aplus no 4080 x 3072 2740 x 2048 no yes C145 yes yes FireWire a FireWire a C Mount 0 63 TV pref Synchronization with external devices configurable via control software FireWire powered 8 Temperature 0 35 C Humidity 5 96 80 96 not condensing Firewire powered 8W Temperature 20 70 C 89 mm x 84 mm x 93 mm USB 145 mm x 93 mm x 123 mm FireWire approx 700 g approx 700 g 800 g ProgRes CapturePro PC MAC TWAIN for PC only ProgRes SDK available at www progres camera com PC MS WIN 2000 XP Vista Mac OS X 10 4 or higher 3 GHz CPU 1 GB RAM 64 MB graphics Firewire a or USB 2 0 ProgRes MAC CapturePro 2 7 Release 001 82 12 3 2 ProgRes CT1 and CT3 ProgRes CMOS cameras Technical specifications 7 Please note ProgRes USB cameras are presently not supported by ProgRes MAC CapturePro ProgRes camera type Image sen
27. C CapturePro by double clicking the ProgRes software icon The software will recognize the connected ProgRes camera during the start and the specific camera data will be loaded e g camera type serial number live image and image capture modes 3 3 Mounting the ProgRes camera to a microscope The camera is equipped with a C Mount interface The camera is mounted to the microscope via this interface For mounting a C mount adapter is used The adapter type depends on the microscope you are using and is not included in the delivery 3 4 Connecting the ProgRes camera to a computer Depending on the camera type the ProgRes cameras can be operated via Firewire IEEE 1394 or USB When the camera is mounted on the microscope set up the connection with the respective USB or firewire cable When operating the ProgRes cameras using MAC OS and ProgRes MAC CapturePro the camera can only be operated via Firewire ProgRes MAC CapturePro 2 7 Release 001 19 Installation and software start Fig 3 5 Connecting the ProgRes camera to a laptop computer via Firewire USB Power supply and data communication are realised via USB for PC and Laptop computers No additional power supply is necessary Connect the camera to your laptop or PC using the USB cable Firewire When operating the ProgRes cameras from a desktop computer power is supplied using the Firewire cable When a laptop computer is used power has to be
28. M 64 MB graphics Firewire a or USB 2 0 ProgRes MAC CapturePro 2 7 Release 001 86 ProgRes C7 Technical specifications 7 Please note ProgRes USB cameras are presently not supported by ProgRes MAC CapturePro ProgRes camera type Image sensor Color Monochrome Sensor resolution max Pixel size A D conversion Pixel clock Dynamic range Exposure times Analog gain Max frame rate image size Image resolution Binning Progr scan Cooling Digital interface Optical connection Trigger In Out Voltage supply Power consumption Ambient conditions Stock conditions Dimensions L x W x H Weight C 1 2 5 CCD Color 3072 x 2304 pixel 7 1 Mpix 1 86 um 12 Bit 32 MHz 60 dB 200 us 5s 1x 16x 11 fps 1228 x 920 pixel 2x 4 614 x 466 1228 x 932 no FireWire a C Mount 0 5x TV pref Synchronization with external evices configurable via control software Firewire powered approx 6 W Temperature 0 35 C Humidity 5 96 80 96 not con densing Temperature 20 70 C 89 mm x 84 mm x 93 mm approx 700 g ProgRes MAC CapturePro 2 7 Release 001 87 Technical specifications ProgRes camera type Software ProgRes CapturePro PC MAC TWAIN for PC only ProgRes SDK External camera driver avallable at www progres camera com Hardware requirements PC MS WIN 2000 XP Vista Mac OS X 10 4 or higher 3 GHz CPU 1 GB RAM 64 MB graphic
29. OPTICAL SYSTEMS LASERS 8 MATERIAL PROCESSING INDUSTRIAL METROLOGY TRAFFIC SOLUTIONS DEFENSE 8 CIVIL SYSTEMS JENOPTIK ProgRes MAC CapturePro 2 7 Manual User advice Dear user Please read the instructions in this manual carefully before starting to operate the software ProgRes MAC CapturePro 2 7 Editorial deadline April 2009 Document number 014102 100 98 02 0904 en JENOPTIK Optical Systems Business Unit Digital Imaging Goeschwitzer Str 25 07745 Jena Germany Tel 49 3641 65 2143 Fax 49 3641 65 2144 E Mail progres jenoptik com Revision state Date Release Revision February 2010 001 002 Note No part of this manual may be reproduced in any form print photoco py microfilm or any other process nor may it be processed duplicated or distributed using electronic systems without a prior written per mission of JENOPTIK The manual was produced with the appropriate care No liability will be accepted in the case of damages arising from the non observance of the information contained herein We reserve the right to modify this document following technical advancements ProgRes MAC CapturePro 2 7 Release 001 2 Signs and symbols Enumeration Advice Important Important advice gt Reference to a text passage or image Warning symbols Warning Warning against the risk of damage or injury Caution Warns against potential damage or malfunction of the pr
30. age screen with gallery By pressing the button Gallery captured images that have been saved in a folder are displayed The folder for the captured images is selected in the dialogue Preferences gt chapter 7 1 Setting preferences In the gallery mode the images of the folder last opened are displayed ProgRes MAC CapturePro 2 7 Release 001 27 Image display and acquisition If the software is reopened the last used gallery will be displayed If another image is captured it will be added to this gallery automatically if no other folder for image storage has been selected 6 2 1 Load an image from the gallery To display an image from the gallery in the main window please click on the respective image in the gallery If you wish to open another gallery please click the button in the icon bar This button is only available in the gallery mode The finder opens where the folder can be selected Er Please note An image stored in a folder can only be loaded in the main window by displaying the images of the folder in the gallery and selecting the respective image from there 6 2 2 Edit an image in the gallery An image in a gallery can be edited by CTRL click on the respective image A dialogue opens that offers the following functions for editing the image Rename Renames an image The new image name can be entered into the text box under the image after selecting this function Click Enter to apply the
31. ancement of the gain In this way the exposure time is reduced and a high frame rate for the live image is achieved The gain can be corrected manually with the respective slider control Note The increase of the gain leads to an increase of the background noise in the image therefore we recommend to activate the Minimize Gain during Capture function to capture optimum images This recalculates the gain in the ProgRes MAC CapturePro 2 7 Release 001 65 Operating panel exposure time during image capture which reduces back ground noise in the captured image The gain adjustment is carried out after the exposure adjustment Analogue Gain All ProgRes cameras are equipped with analogue gain Analogue gain is carried out directly in the camera and is applied to regions 1 to 8 The image signal is enhanced through the use of analogue gain upstream of the ADC in the camera and the background noise is red uced at the same time In regions gt 8 the signal is additionally digitally enhanced Note In order to achieve the best possible quality for image capturing activate Minimize Gain during Capture This means that a gain value of 1 is applied to the captured images and the exposure time is increased correspondingly The background noise in the captured image is reduced The maximum gain values are different for various ProgRes cameras depending on the maximum exposure time available with the
32. apse stopped appears at the end of the list If the saving of the protocol is activated in the index card Preferences Timelapse the protocol will be saved as a text file in the same folder which has been defined for the captured images 7 Note When the function As fast as possible is selected the images are saved during the transfer from the camera to the mo nitor This saving procedure achieves high frame rates however the saving intervals are not constant When the time controlled image saving is selected constant frame rates are achieved The Live mode is stopped the image resolution is adapted and the image is saved in the directory Be cause of these intermediate steps it might occur that the frame rates are slower than selected depending on the performance of your system Please contact the technical support in case of any problems ProgRes MAC CapturePro 2 7 Release 001 46 7 3 Calibration With a click on the button Calibration E a window opens enabling the following calibration functions Black shading calibration White shading calibration Scanner calibration The following functions are available in the calibration window Information Displays the camera type the serial number and the firmware version number Black Shading Calibration With a click on this button a black shading calibration is started With a click on Reset Black Shading the calibration is reset Mark
33. apturePro 2 7 Release 001 70 Technical specifications 12 Technical specifications of ProgRes cameras 7 Please note In the following chapter the specifications of all ProgRes microscope cameras are shown Please note that pre sently only ProgRes microscope cameras with a Firewire inter face can be operated using MAC OS and the software ProgRes MAC CapturePro If you would like to work with a USB camera please contact your expert dealer or the technical support of the manufacturer 12 1 Generals 12 1 1 plate and order numbers Serial number and additional information can be found on the label on the rear side of the camera C CE aser IK oysteme Gm 0 07739 Jena ProgRes C10plus E190363 S N 3006 00 0103 42 V 9 33V DC ITE 85ML gm P ew Made in Germany Fig 12 1 Type plate and order numbers Order number of ProgRes cameras Camera type Order number ProgRes C3 014102 004 26 ProgRes 014102 005 26 ProgRes C5 014102 008 26 ProgRes C5 el 014102 009 26 ProgRes C7 014102 015 26 ProgRes CT3 014102 001 26 ProgRes CF 014102 656 26 ProgRes 014102 654 26 ProgRes MAC CapturePro 2 7 Release 001 71 ProgRes CFs ProgRes MF ProgRes ProgRes ProgRes 14 5 014102 653 26 014102 657 26 014102 651 26 014102 655 26 014102 650 26 Technical specifications Order numbers of ProgRes CMOS cameras Camera type Order number ProgR
34. are selected in the index card Preferences Timelapse gt chapter 7 1 4 Timelapse 4 ProgRes File Edit Camera Window A 4 0950 p 2 AMAN gt men Orten EI fo Fig 7 14 Image display with drawer Timelapse When the button Start Time Lapse now is clicked the time controlled image capture is started immediately When Start Time Lapse delayed is clicked a window opens where the delay of the start of the image capturing can be inserted With a click on Start the Count down to the start of the image re cording is started and displayed in a window With a click on Cancel the process can be cancelled ProgRes MAC CapturePro 2 7 Release 001 45 Operational windows Timelapse Hours 8 Minutes 00 8 Remaining 00 00 06 Seconds 10 Current Time 19 04 2007 11 35 48 Uhr D 00 00 10 Start Time 19 04 2007 11 35 54 Uhr Current Time 18 04 2007 16 36 10 Uhr Cancel Start Time 18 04 2007 16 36 20 Uhr ED Fig 7 15 Insert delay of image capture Fig 7 16 Display of remaining time to start The progress of the image recording can be viewed in a display window in the image and in the protocol in the window Timelapse In the window the recorded images with the capture time are listed When the time lapse image capture is complete Timel
35. be lt cm gt T lt m gt selected When a new unit is selected it will be immediately updated in the image Scale Length Fig 7 20 Window Measurement calibration With a click on the buttons existing calibration or unit can be added or deleted With a click on the button Edit an existing calibration or unit can be edited In the area Scale the length of the previously set scale is displayed using the currently selected unit Note The change of the units is only possible when a user defined calibration has been set 7 4 1 Create or edit a calibration The calibration of the measurement units is carried out with a line for referencing distances in the image The calibration Pixels is set by default Unless a user defined calibration is created all distances in the image are displayed in pixels ProgRes MAC CapturePro 2 7 Release 001 54 Operational windows Measurement Place the calibration slide under the microscopic lens Click on the button to add a new calibration In the image window a line will appear Using the mouse the length and position of this line can be set that serves as a reference for the new calibration To edit an existing calibration mark the calibration and click the button Edit The window New Calibration opens and displays a line New Calibration SEANS Use the line tool to describe a distance of 0 01 mm i Name New Ca
36. binding ProgRes MAC CapturePro 2 7 Release 001 73 Technical specifications 12 2 Image resolution of the ProgRes cameras ProgRes C7 Resolution Live Capture 3072 x 2300 X 3072 x 2300 Shut 1228 920 HFRM x x 614x460 HFRM x X 1228x9324xBin X 614 x 466 4x Bin X X 306x2324xBin X ProgRes CT3 Resolution Live Capture 2048 x 1536 X 1024 768 2x Bin 682 x 512 3x Bin x ProgRes C3 Resolution Live Capture 2080 x 1542 HQ 2080 x 1542 1 692 x 514 VGA 1040 x 770 2x Bin 692 x 514 3x Bin 520 x 384 4 Bin 416 x 208 5x Bin x x x X x x x ProgRes MAC CapturePro 2 7 Release 001 74 Technical specifications ProgRes C5 Resolution Live Capture 2580 x 1944 HQ 1 2580 1944 1290 x 972 VGA x 644 x 490 VGA 1290 x 972 2x Bin 860 x 648 3x Bin 645 x 486 4x Bin 516 x 389 5x Bin x X XxX x ProgRes CF Resolution Live Capture 1360 x 1024 HQ X 1360 x 1024 X X 680 x 512 680 x 512 HFRM 340 x 256 HFRM 452 x 340 3x Bin 272 x 204 5x Bin 1 x x x ProgRes CFs Resolution Live Capture 1360 x 1024 HQ x 1360 x 1024 680 512 X 680 x 512 HFRM 340 x 256 HFRM 4080 x 3072 9x Scan 2040 x 1536 452 x 340 3x Bin 272 x
37. can be adjusted A low gamma value renders a brighter high contrast image a high gamma value renders a darker image with lower contrast The gamma adjustment does not reduce the dynamic range of the image The preset value for gamma settings is 0 5 which results in well balanced contrasting color reproduction For a linear reproduction of the image data select a gamma value of 0 33 Saturation The saturation of the image can be adjusted by using the saturation slider control 8 2 Adjust balance With the slider controls the color balance in the entire image can be adjusted between an RGB base color and the respective complemen tary CMYK color The color balance adjustments are retained when a white balance is applied to the image with the pipette tool ProgRes MAC CapturePro 2 7 Release 001 63 Operating panel 8 3 Image resolution In the Acquire mode the image resolutions for the live and the cap tured images can be selected The drop down list opens after clicking the arrows besides the boxes Live and Capture 1360 x 1024 1360 x 1024 680x512 HFRM 1360 x 1024 F 340 x 256 HFRM 680 x 512 HFRM 453 x 340 3x Bin 340 x 256 HFRM 272 204 5 Bin 4080 x 3072 36x Scan 4080 x 3072 9x Scan 2720 x 2048 16x Scan 1360 x 1024 4x Scan 453 x 340 3x Bin 272 204 5x Bin Fig 8 2 Image resolution in Live and Capture mode The image resolutions vary according to the camera ty
38. ce can aD be adjusted Capture 2080 x 1542 F m posure o u m 1 zZ In the Acquire mode the resolution for Een live and captured images and the exposure C Auto txposure and gain settings can be set A histogram visualises the distribution of the color values in the image The color values can also be changed in the histogram by moving the slider bars 4 255 Fig 8 1 Operating panel in the Acquire mode 8 1 Adjust brightness contrast gamma and saturation The image parameters contrast brightness gamma and saturation can be adjusted to the specific requirements of the user in the live images or in captured images loaded from the gallery The image display and the histogram are updated immeditately The settings can be adjusted using the slider control and can be reset with a click on the button Reset Changes to the image parameters are saved as user preferences and will be available at the restart of the software ProgRes MAC CapturePro 2 7 Release 001 62 Operating panel Contrast Move the slider control to increase or reduce the contrast in the live image The changes are immediately applied to the image display A contrast value of 1 0 is preset in the image Brightness Brightness in the image can be adjusted by moving the slider control Gamma With the gamma slider control the conversion of camera pixel values into the pixel values displayed in the image
39. d The images can be stored as a Apple VCH 263 sequence of single images or as a Er US QuickTime Movie DVIDVERRO NTSC DVCPRO PAL 1 1 CPROSO NTSC Both formats will be stored in the folder est n u ar Graphi specified under Save images in 263 When the images captured as 2000 4 Motion JPEG A quence of single images the file format as Motion JPEG B re ideo selected on the index card Acquire gt chapter 6 1 Acquire mode will be used N Sorenson Video For a QuickTime Movie the format can Sorenson veo be specified with a click on the button as Setup A menu opens where the format fig 7 8 Select movie format for the movie can be selected 7 1 4 3 Save Protocol The settings for saving the protocol of the time controlled image cap turing can be defined The protocol is saved as a text file together with the captured images in the selected folder Auto Save A protocol is always saved with the captured images ProgRes MAC CapturePro 2 7 Release 001 40 Operational windows Setting preferences Never No protocol will be saved with the images Ask user The user will be asked whether the protocol is to be saved with the images 7 1 4 4 Time Control Time Control Use Minimal Possible Interval Use Time Settings Total Time and Interval Time Total Time 00 00 05 000 8 in hh mm ss msec Interval Time 00 00 00 50
40. date is available 1 2 Intended operation of the ProgRes cameras ProgRes microscope cameras are intended for the operation with a microscope and for the operation and image analysis with the suitable software Application software is available for the operating systems MAC OS and WINDOWS In this manual the software for MAC OS is described SE Important note Presently only ProgRes microscope cameras with a Firewire interface can be operated using MAC OS and the software ProgRes MAC CapturePro If you would like to work with a USB camera please contact your expert dealer or the technical support of the manufacturer ProgRes cameras are suitable for applications in science and research as well as in production that require high quality microscopic image reproduction Typical application fields are Material science metallography quality control Life science genetics pathology biology Forensics securing of evidences ProgRes digital cameras are designed and manufactured for the use in clean and dry locations according to EN60950 They are intended for 1 digital image acquisition Any use in human medical technology accor ding to IEC601 1 1 is not allowed and herewith is excluded explicitly ProgRes MAC CapturePro 2 7 Release 001 8 Introduction 13 Feedback approval criticism suggestions We strongly rely on your most welcome feedback for improving our products according to your needs Please let us k
41. e focus and carry out a white shading if necessary Apply black shading The black shading function is available if a monochrome camera is used or if the black and white mode is selected The black balance serves for reducing auto fluorescence in the image The pipette tool for the white balance will change to a black pipette and can be used to perform a black balance gt chapter 6 3 5 Black With this pipette tool the areas in the image with grey values of the selected spot are set to black RGB values 0 Adjust image settings In the operating panel image resolution exposure gain contrast brightness and gamma can be adjusted in the fluorescence mode gt chapter B Operating Panel When you are pleased with the image click the button Capture After capturing the image a dialogue appears requesting you to acti vate the next filter The process will be repeated until the last image is captured ProgRes MAC CapturePro 2 7 Release 001 58 Operational windows Fluorescence 7 5 2 Adjust captured fluorescence images After the completion of the fluorescence project the captured images are displayed in a gallery under the main image display and the area Gallery becomes active under the fluorescence image display The name of the filter used for capturing the image is automatically assi gned to the respective image ProgRes File Edit Camera Window 93 9 BS sx 3 1 1009
42. e standard MAC functions for file handling ProgRes MAC CapturePro 2 7 Release 001 24 5 3 The menu Edit Undo 2 The menu Edit offers the standard MAC Redo 082 ae options for editing Cut 3X Copy Paste 36V Delete Select Special Characters Fig 5 4 Menu Edit 5 4 The menu Camera ec Window Acquire Gallery Reset Camera to Factory Settings Fig 5 5 Camera menu Acquire Displays the live image screen From here images can be ac quired gt chapter 6 1 Acquire mode Gallery Displays the captured images in a gallery The images can be displayed and edited gt chapter 5 2 Display saved images in a for further information 5 5 The menu Window This menu contains options concerning the size and the display of the general application window as preset in the MAC operating system ProgRes MAC CapturePro 2 7 Release 001 Menu bar 25 Image display and acquisition 6 Image display and acquisition Fig 6 1 Main section buttons The image acquisition and the gallery display are carried out using the buttons Acquire and Gallery 6 1 Acqire mode Live image display ProgRes File Edit Camera Window 4 ER ProgRes Mac CapturePro 2 6 1 WC Joa E go image HeLa s Decor UwrsiStmci Deko x 2 as
43. es CT1 USBM 014102 030 26 ProgRes CT1 USB 014102 031 26 ProgRes CT3 USB 014102 032 26 ProgRes CT5 USB M 014102 033 26 ProgRes CT5 USB C 014102 034 26 Order numbers of ProgRes CCD cameras Camera type Order number ProgRes MS USB 014102 035 26 ProgRes CS USB 014102 036 26 ProgRes MF USB 014102 037 26 ProgRes CF USB 014102 038 26 12 1 2 Scope of delivery Camera ProgRes FireWire or USB cable Stand alone software amp TWAIN driver for WINDOWS PC Stand alone software for MAC Operation manual on CD and printed e Thread sleeve 3 8 to 4 photo thread Protective cap for C Mount Delivery in durable hard case Additional to ProgRes CF ProgRes MF and ProgRes 14 5 Calibration slide Cleaning brush ProgRes MAC CapturePro 2 7 Release 001 72 Technical specifications 12 1 3 Certificates and approvements CE according to EMC guideline 2004 108 EG Limit values for FCC are met 12 1 4 System requirements CPU Macintosh G4 or G5 Intel or PPC Processors 1 GHz or higher recommended Memory 512 MB Graphics 64 MB Operating System MAC OS X 10 4 or higher Interface IEEE1394a FireWire onboard cr Note It is recommended to deactivate the Power Save settings of your computer while working with the ProgRes camera It is our policy to constantly improve the design and specifications Accordingly the details represented herein cannot be regarded as final and
44. g the button Setup a dialo Donitrecompress gue is opened where the settings for Best Depth MM the respective file format can be de fined This dialogue varies according E ES TER to the selected format O Target size Fig 7 5 shows the options for a JPG image Cancel Fig 7 5 Adjustments 7 1 2 2 Auto Save f Auto Save is enabled by marking the respective checkbox the cap tured images will automatically be saved in a folder without a request or file name and location The folder can be specified by clicking the button Choose folder The name of the folder will be displayed below the checkbox over the buttons Choose Folder Chooses the folder where the captured images will be stored automatically Default Folder Creates a folder on your desktop with the current date as folder name ProgRes MAC CapturePro 2 7 Release 001 36 Operational windows Setting preferences Show Folder Opens the folder you have selected for Auto Save Note To open a captured image the folder where the image is stored has to be displayed as a gallery The image can be shown in the display window by selecting it from the gallery chapter 6 2 Display saved images in a Gallery 7 1 2 3 Auto Open If Auto Open is enabled the captured images will be stored tem porarily on the hard drive of your computer and will be automatically opened with the selected targe
45. ge sensor 1 2 CCD 2 3 CCD Color Monochrome Color Color Sensor resolution 2080 x 1542 pixel 2580 x 1944 pixel max 3 3 Mpix 5 0 Mpix Pixel size 3 45 um 3 4 A D conversion 12 Bit 12 Bit Pixel clock 12 MHz 12 MHz 18 MHz Dynamic range 61 dB 61 dB 60 dB ProgRes MAC CapturePro 2 7 Release 001 85 ProgRes camera type Exposure times Analog gain ax frame rate im age size mage resolution Binning Progr scan Cooling Digital interface Optical connection Trigger In Out Voltage supply Power consumption Ambient conditions Stock conditions Dimensions L x W x H Weight Software External camera driver Hardware require ments Technical specifications C3 5 500 us 180 5 400 us 180 5 1 8 1 8 18 fps 1040 770 9 fps 1290 x 972 pixel pixel 2x 5x 2X DX 346 x 256 692 x 644 x 490 1290 512 x 972 optional optional FireWire a FireWire a C Mount C Mount 0 5x TV pref 0 63x TV pref Synchronization with external devices configurable via control software FireWire powered FireWire powered approx 4 W Temperature 0 35 C Humidity 5 96 80 not condensing approx 6 W Temperature 20 70 C 89 mm x 84 mm x 93 mm approx 700 g ProgRes CapturePro PC MAC TWAIN for PC only ProgRes SDK available at www progres camera com PC MS WIN 2000 XP Vista Mac OS X 10 4 or higher 3 GHz CPU 1 GB RA
46. hop JPEG and JPEG 2000 25 1 2 Acquire Image Info Acquire User interface Time Lapse Measurement Fluorescence Prefix amp Format Prefix image_ Format TIFF 18 Auto Save Directory Volumes PROGRES ROE Fluoreszenz FluoSessions FOM Choose Folder _ Default Folder Auto Open Application n a Choose Target Clear Target Show Target Launch Target Target to Front Quit Target Options Autoexposure Preserve Current Gain Optimize Exposure amp Gain Reset to Factory Settings ED Fig 7 3 Dialogue Preferences Acquire n this register card the storage location of the captured images the target application for working with ProgRes TWAIN and the image options can be defined 7 1 2 1 Setting prefix and format Enter an image name prefix for image saving When Auto Save is enabled images will be saved automatically using the name prefix combined with an ascending number ProgRes MAC CapturePro 2 7 Release 001 35 Operational windows Setting preferences sci Click onto the arrow buttons to open the Photoshop dialogue for selecting the file formats The file BMP formats and bit depths that can be selected JPEG are shown in Fig 7 4 Storage formats PICT PNG PNG 16 Bit TIFF TIFF 16 Bit TGA 2 QuickTime Image Fig 7 A Storage formats JPEG Options Clickin
47. ics Firewire a or USB 2 0 7 Please note ProgRes USB cameras are presently not supported by ProgRes MAC CapturePro ProgRes camera type Image sensor Color Monochrome Sensor resolution max Pixel size A D conversion Pixel clock Dynamic range Exposure times Analog gain Max frame rate image size Image resolution Cooling Digital interface CT5 1 2 5 CMOS Color Monochrome 2592 x 1944 pixel 5 Mpix 2 2 um 12 Bit 48 MHz 68 dB 75 5 55 1x 8 6 fps 2592 x 1944 pixel 2x 3X no USB 2 0 Binning ProgRes MAC CapturePro 2 7 Release 001 84 ProgRes camera type Optical connection Trigger In Out Voltage supply Power consumption Ambient conditions Stock conditions Dimensions L x W x Weight Software External camera driver Hardware requirements Technical specifications 5 C Mount 0 5 TV pref Synchronization with external evices configurable via control software USB powered approx 1 8 W Temperature 5 C 55 C Humidity 5 80 96 not con densing Temperature 20 70 C 89 mm x 84mm x 93 mm approx 700 g ETUR CapturePro PC MAC TWAIN for PC only ProgRes SDK available at www progres camera com PC MS WIN 2000 XP Vista Mac OS X 10 4 or higher 3 GHz CPU 1 GB RAM 64 MB graphics Firewire a or USB 2 0 12 3 3 ProgRes CCD routine cameras ProgRes C3 and C5 ProgRes camera type C3 5 ma
48. ion has been preset by the manufacturer however a re calibration should be carried out occasio nally to achieve an optimum image quality How does scanning work During image capturing in the micro scanning mode 4x shot 9x shot 16x shot 36x shot the sensor is moved between the individual images by fractions of a micrometer In 4x shot micro scanning the sensor is displaced by exactly one pixel width in 16x and 36x shot the CCD sensor is moved by only one half or one third of a pixel width ProgRes MAC CapturePro 2 7 Release 001 49 Operational windows Calibration From these individual images captured by the sensor movement one high resolution final image is calculated by the software An extremely high precision of the displacement is therefore a prerequisite for micro scanning During calibration the camera first captures a high resolution image of the calibration pattern in the micro scanning mode Calibration patterns are included in your delivery Within a section of this image the extent of the displacement of the individual images is determined by the pattern structure Using these data the voltage values for the piezo ac tuators are calculated and corrected if necessary If the data exceed or fall below specific limit values a new high resolution image is captured in a further iteration stage and the calculation is carried out again with the consideration of the values of the previous stage This process wil
49. l be repeated until the scanner is successfully calibrated Why is calibration necessary The scanner displacement is controlled by a mechanical system using piezo actuators known as scanners These actuators consist of piezo electric crystals which deform slightly when subjected to electric cur rent This slight deformation is sufficient to achieve the microscopically small displacement necessary for micro scanning The extent of this deformation at a particular voltage is individual for each element and can be affected by external factors such as environment temperature and humidity Since the displacement has to be carried out precisely and reproducibly the scanners have to be calibrated occasionally When should a scanner be calibrated The scanner is calibrated by the manufacturer and can be used im mediately This preset calibration is permanently saved in the camera EPROM and is as a basic principle recommended for re calibration A re calibration may be necessary if Environmental conditions e g temperature or humidity have changed considerably since the last calibration The camera has not been in use for a long time e The image quality has deteriorated in the micro scanning mode ProgRes MAC CapturePro 2 7 Release 001 50 Operational windows Calibration If the camera is used under normal conditions and if the environmental conditions have not changed it is usually sufficient to check the calibra
50. libration ne ED Fig 7 21 Set a new calibration The line has to be configured adequately for the calibration The size of the calibration line can be changed by clicking on the blue dots and by pulling the mouse The place of the line in the image can be changed by a click on the line and by pulling the mouse In the lower part of the window the distance and the unit can be inserted corresponding to the line and the name of the calibration can be inserted Click OK to apply the settings and to create the new calibration ProgRes MAC CapturePro 2 7 Release 001 55 Operational windows Measurement If a calibration has been edited the existing data will be overwritten Click Cancel to reset the changes made in the window The name of the calibration will appear in the area Calibrations in the window The selected calibration will now be used for referencing the length of the measurement shapes in the image The desired calibra tion can be selected by a click onto the name of the calibration in the respective area of the measurement window 7 4 2 Insert anew unit or edit a unit In the window Units the available units are displayed When a new unit is selected the reference of the distances for the shapes in the image is updated immediately The following units are set by default um mm cm m inch and mil If further units are desired for example country specific measurement units they
51. mental conditions Keep it away from extreme temperatures high humidity fluids chemical gases or high electroma gnetic fields Details can be found in the technical specifications Static electricity Static electricity can damage or even destroy the delicate electronics of your camera Before you connect the camera to a computer or a microscope please make sure that it is free of electrostatic charge Ground yourself by touching the metallic housing or the reverse side of your computer or microscope which both have to be grounded via a power socket ProgRes MAC CapturePro 2 7 Release 001 A 11 Safety instructions Ventilation Ensure the sufficient ventilation of the camera Make sure that the louvers on the camera back are not covered Avoid direct sun exposure and the location nearby any heat sources radiators stoves Overhea ting can cause poor image quality To avoid the risk of fire do not use the camera near inflammable liquids or gases Transportation The camera has to be protected against mechanical impact and there fore may only be transported or stored in the supplied carrying case When the camera is transported or stored please use the supplied C mount cover 2 3 Application fields of the ProgRes cameras ProgRes digital cameras are designed and manufactured for the use in clean and dry locations according to EN60950 They are intended for digital image acquisition Any use in human medical techn
52. mputer it is necessary to install the appli cation software which also includes drivers for the ProgRes cameras 3 1 Installing the software ProgRes MAC CapturePro Double click on the installer icon and follow the instructions displayed on the screen The installation routine is started Important Information Copyright Jenoptik AG 2001 2008 Introduction For software updates and registration please visit our ProgRes Camera web Read Me site www progres camera com License Destination Select a ProgRes CT3 ProgRes CIOPIus ProgRes C5 C12Plus Summary ProgRes 7 ProgRes CF ProgRes CF ProgRes crean ProgRes c aPlus ProgRes MF ProgRes MF rt H ProgRes Prog Res css f Save C Go Back 3 Continue Fig 3 1 Installation Readme screen Installation Type 8 Installation The readme screen provides information about the available ProgRes cameras the system requirements and the contact data Scroll down to read the respective data Click Continue ProgRes MAC CapturePro 2 7 Release 001 16 Installation and software start To continue installing the software you must agree to the terms of the software license agreement Click Agree to continue or click Disagree to cancel the installation and quit the Installer Cose Cre Installation
53. n i e the chequerboard pattern does not have to be parallel to the window edges Check the adjustment of the calibration pattern in the live image Pattern Within the image frame several black and white squares have to be visible to check the transitions The image frame will be visible if Show Calibration Rectangle has been activated in the window Calibrati on If necessary the magnification the focal depth of the lens or the distance of the pattern can be changed Exposure The pattern should be amply exposed however overexposure has to be avoided Optimum exposure can be achieved by a click on AutoEx posure in the operating panel Sharpness For a successful scanner calibration the calibration pattern must be slightly out of focus Once the pattern has been set up the calibration can be started ProgRes MAC CapturePro 2 7 Release 001 52 Operational windows Calibration Start calibration Click on the respective button to Scanner Calibration start the calibration When the 4x Scanner User Calibration 3 a scanner calibration has been suc 9 x 5 User Calibration un cessful the software displays the 16 x Scanner F Calibrati R 5 date of its creation The software 36 x Scanner Factory Calibration automatically uses the calibra tion files If you create a new scanner calibration the software will overwrite the existing file Activate Co
54. name Duplicate Duplicates the image The image will be automatically added to the images in the gallery Delete Deletes the selected image Show in finder Shows the storage place of the selected image Show image info Shows the information saved with the image The image information ProgRes MAC CapturePro 2 7 Release 001 28 Image display and acquisition can be set under Image info in the dialogue Preferences gt chapter 7 1 Setting Preferences for further information To leave the gallery mode click Acquire 6 3 The icon bar With the buttons of the icon bar settings for the image display can be defined and a measurement layer can be applied 6 3 1 Display exposure warnings With a click on the button Exposure warning the areas in the image which are under or overexposed will be displayed in color The colors have to be selected and the display of these areas has to be activated in the file card User interface under Preferences gt chapter 7 1 3 User interface 6 3 2 Zoom and rotation ag With the Zoom buttons the image size can be enlarged or reduced If the image is larger than the display window scrollbars appear on the right and the lower side of the image and can be used to navigate within the image 12 5 The function Zoom to fit enables the adaptation of the 16 7 200 microscopic image to the image window in the software 25 0 E
55. nces can also be set in the menu ProgRes Timelapse Set time controlled image capture and saving Calibration Perform shading and scanner calibration easure Select settings for measurement in the image Fluorescence Perform fluorescence image capturing non Note The functions Timelapse Calibration and Measure are not available in the Gallery mode ProgRes MAC CapturePro 2 7 Release 001 33 Operational windows Setting preferences 7 1 Setting preferences In the dialogue Preferences settings for the image acquisition and measurement as well as display settings for the user interface can be selected The screen Preferences is divided into 6 register cards Clicking Preferences automatically opens the last register card left with With the first software start the register card Image Info is opened Image Info _ Acquire User interface Time Lapse Measurement Fluorescence Fig 7 1 Menu bar in the dialogue Preferences It is recommended to adjust your camera and microscope according to your requirements before starting your working session All settings will influence the operation of the camera and will be automatically saved when closing the software After restart these adjustments will be available again The following buttons are available in each index card With Reset all settings in the image are reset to factory settings With
56. ng of the camera With a click on the button Help a window opens displaying detailed explanations of the respective calibration procedure 7 3 1 Black shading calibration With a black shading calibration the sensor noise at exposure times gt 1 sec second can be reduced by subtracting a black reference image from the captured image 1 Ensure that no light is falling onto the CCD sensor It may be necessary to remove the camera from the microscope and to put the CCD protection cap on or to place the camera onto an even ground 2 With a click on Black Shading the black shading algorithm is started After the black reference has been carried out successfully the software will display the date 3 Markthe checkbox Apply black shading if available if you would like to apply the black shading correction to the live images 4 The background correction will be applied automatically to any newly captured images until it is switched off by removing the checkmark from the Apply checkbox 5 Ifa new black shading correction is created the software will over write the existing file automatically em Note The background correction will not be applied to images in binning mode 7 3 2 White Shading Calibration With a white shading calibration irregularities in the image area caused by boundary light falloff in the optical system vignetting or by non homogeneous lighting can be compensated ProgRe
57. now what you like about the ProgRes cameras and the ProgRes MAC CapturePro soft ware tell us what you dislike and also what you are missing We will try to consider your feedback in the further development of our products and in this way we will let you benefit from such improve ments Please send your feedback to progres jenoptik com We wish you every success with your ProgRes microscope camera JENOPTIK Optical Systems ProgRes MAC CapturePro 2 7 Release 001 Safety instructions 2 Safety instructions The ProgRes digital microscope camera is manufactured and tested ac cording to the regulations specified in EN60950 VDE0805 and was in perfect working condition when leaving the manufacturer s premises In order to maintain this standard and to avoid any risk when opera ting the system please make sure to comply with the advice and the warnings contained in this manual No warranty will be assumed by the manufacturer during the warran ty period if the equipment is operated without observing the safety regulations In any such case JENOPTIK shall be exempt from statutory liability for accidents resulting from any operation Please read this chapter carefully and observe the regulations in order to ensure your safety and to operate the ProgRes camera as intended Please observe the warnings and notes printed in this manual and on the unit 2 1 General safety information Expansions and alterations The
58. ntly Used Charcoal CY Bold 10 Chinesisch Cochin Bold 11 Feste Laufweite Comic Sans MS 12 Japanisch Copperplate T Klassiker Corsiva Hebrew 14 Koreanisch X Courier la 18 4 Moderne Courier New E z4 5 er Search Fig 7 12 Select settings for the font of the overlay ED Fig 7 11 Set line and font color Scale Sets the display settings of the scale Using the arrow buttons the number of subdivisions max 20 can be altered 5 subdivisions are set by default By marking the checkbox Show Frame the scale will be displayed with a frame A scale for referencing distances in the image can be set by clicking E in the icon bar Clicking the arrow buttons of the function enables the subsequent selection of a horizontal or vertical scale Label Marking the checkbox Show Length the length of the scale and the measurement elements in the image are displayed Images When the checkbox is marked grayscale images are saved as color images ProgRes MAC CapturePro 2 7 Release 001 43 Operational windows Setting preferences 7 1 6 Fluorescence Image Info Acquire User Interface Time Lapse Measurement Fluorescence Filters Dye Emission nm Color Active 1 FITC 550 a 2 TRITC 610 3 DAPI 488 E M Edit Options Prefix for Merged Images n a New Filter Dye New Filter Emission in nm 390 54 Active Gree ED Reset to Factory Se
59. ntrol software USB FireWire po wered Trigger In Out Voltage supply USB powered Power consumption approx 2 4 W 2 5 5 W Firewire ProgRes MAC CapturePro 2 7 Release 001 80 ProgRes camera type Ambient conditions Stock conditions Dimensions L x W xHl Weight Capture software External camera driver Hardware requirements Technical specifications 5 MS Temperature 0 35 C Humidity 5 80 not condensing CF MF Temperature 20 70 C 89 mm x 84 mm x 93 mm USB 45 mm x 93 mm x 123 mm FireWire approx 700 g approx 700 g 800 g TWAIN for PC only ProgRes SDK available at www progres camera com PC MS WIN 2000 XP Vista Mac OS X 10 4 or higher 3 GHz CPU 1 GB RAM 64 MB graphics Firewire a or USB 2 0 ProgRes CF ProgRes CFs MF and ProgRes 1 495 ProgRes camera type Image sensor Color Monochrome Sensor resolution max Pixel size A D conversion Pixel clock Dynamic range Exposure times Analog gain Max frame rate image size Image resolution Binning ProgRes MAC CapturePro 00 2 3 progress scan Color Monochrome 1360 x 1024 pixel 8 1 Aplus 2 3 CCD progress scan Color Monochrome 1360 x 1024 pixel 1 4 Mpix 11 4 12 5 Mpix 6 45 um 6 45 um 14 Bit 14 Bit 12 MHz 24 5 MHz 12 MHz 24 5 MHz 67
60. oduct Information Refers to important data or details This symbol refers to special guidelines that have to be followed when disposing the product ProgRes MAC CapturePro 2 7 Release 001 User advice EP Contents Table of contents Signs nd symbols u 3 Warning symbols sssssssse e 3 1 Introduction ES 1 1 8 0116 cerdo BEHRENS ioe spit ds secet Habet 7 1 2 Intended operation of the ProgRes cameras 8 1 3 Feedback approval criticism suggestions 9 2 Safety instructions 0 2 1 General safety information 0 2 2 Operating storage and transportation conditions 1 2 3 Application fields of the ProgRes cameras 2 24 Electrical installation 2 2 5 Advice for handling and operating the camera 3 2 6 Cleaning and 3 2 7 Exemption from statutory liability for accidents 4 2 8 Exemption from warranty 4 2 9 Safety note for use in the United States of America 4 2 10 Disposal osito cs 5 3 nstallation and software 6 3 1 nstalling the software ProgRes MAC CapturePro 6 3 2 Starting the ProgRes MAC
61. oling automatically Factory Calibration Show Calibration Rectangle Fig 7 19 Scanner calibration field f you click the Factory Calibration button all calibration files are deleted and set to factory calibration values Important note It is important to ensure that camera and pattern are well secured and are not subject to any vibration 7 3 4 Activate cooling You can mark the checkbox to activate the cooling peltier element and fan for the cooled cameras ProgRes 1495 ProgRes CFan ProgRes CF ProgRes and ProgRes MF When the software is first started the cooling is deactivated to reduce the camera power consumption ProgRes MAC CapturePro 2 7 Release 001 53 Operational windows Measurement 7 4 Measurement window Clicking the button a measurement window opens for carrying out measurement calibrations With these measurement calibrations distances and units in the image can be referenced In the measure ment calibration window distances can be set and edited In the area Calibrations the available MER calibrations are listed Pixels is the standard calibration preset in the facto 4 0 x 0 01mm When this calibration is selected the lengths of the shapes in the image are shown in pixels 1 1 uote In the area Units the units of the lt um gt shapes displayed in the window can
62. ology accor ding to IEC601 1 1 is not allowed and herewith is excluded explicitly 2 4 Electrical installation conditions The electrical installations of the room where the system is to be set up must be in accordance with IEC requirements Voltage 8 33 VDC via IEEE1394 connector supply Power con Variable according to the camera type Please refer to sumption the technical data sheet of your camera in chapter 12 in this manual To interrupt the power supply of your camera please unplug the IEEE 1394 FireWire cable Only use the cables supplied by the manufacturer No warranty will be assumed for damages or injuries arising from the non compliance with this advice Do not allow any cables particularly power cords to trail ProgRes MAC CapturePro 2 7 Release 001 12 Safety instructions across the floor The electrical cables have to be protected against mechanical impact Connect and disconnect the cables by pulling the connector 2 5 Advice for handling and operating the camera Your digital ProgRes camera is an optical fine mechanical precision device Please handle it with the due care The reliability and perfor mance as well as the longevity of the system can only be guaranteed if all safety instructions are observed The built in IR filter glass has to be protected against mechanical impact such as shock or scratching as well as against dirt Do not remove the ferri
63. pe refer to chapter 12 2 Image resolution of the ProgRes 5 for detailed information about the image resolution of your camera 8 4 Adjustment of exposure and gain 8 4 1 Adjust exposure Exposure can be adjusted in the Acquire mode Use the slider control Exposure to adjust the exposure in the image Behind the slider the value is automatically displayed in ms By clicking the button Auto Exposure the optimum exposure of the image is adjusted automatically ET Note All automatic settings will be reset if you use the expo sure is adjusted manually ProgRes MAC CapturePro 2 7 Release 001 64 Operating panel The maximum exposure time values for the different ProgRes cameras are listed in the following table Camera type Max exposure time ProgRes C3 180 ProgRes C5 180 ProgRes C7 5 ProgRes CT3 3 ProgRes C1 4pls 600 ProgRes MF 180 ProgRes 300 ProgRes 300 ProgRes CF 180 ProgRes Cro 300 ProgRes CFS 300 ProgRes CS USB 80 ProgRes MS USB 80 ProgRes CF USB 180 ProgRes MF USB 180 ProgRes CT1 C USB 0 522 ProgRes CT1 M USB 0 522 ProgRes CT3 USB 3 ProgRes CT5 C USB 3 ProgRes CT5 M USB 8 4 2 Adjust gain When applications are carried out under very weak light conditions for example dark field or fluorescence applications the image signal can be amplified by an enh
64. r the setting of the image resolution of the live image and the captured image and for the adjust ment of exposure and gain Furthermore the setting of image contrast brightness and saturation and the adjustment of the color channels can be selected The color channels are visualized in a histogram in the lower part of the panel The operation panel is described in more detail in chapter B Operating Panel Image description area pink frame In this area comments to the captured image and the image prefix can be entered These data are stored when the image is captured by pressing the button Acquire Please refer to chapter D Image Bescription area for more details Image information bar black frame This bar indicates the current image values like cursor position frame rates or the camera type connected The image information bar is described in chapter 10 Image information bar The functions of the software user interface will be described in more detail in the following chapters ProgRes MAC CapturePro 2 7 Release 001 22 5 Menu bar ProgRes Fig 5 1 Menu bar Menu bar File Edit Camera Window The menu bar contains general features and functions 5 1 The menu ProgRes About ProgRes Mac Capture Pro Preferences Reset to Factory Settings Load Settings Save Settings Services Hide ProgRes 36H Hide Others Show Al Quit ProgRes Q Fig 5 2 Men
65. rectory Users G4Lapi Pictures Images Capture Pro Capture Test 16 04 2007 15 54 51 Choose Folder Default Folder 7 ShowFolder Save Images as O Single Images Single Images uses the QuickTime Movie Acquire Prefix amp Format settings Save Protocol Auto Save O Never O Ask user Time Control O Use Minimal Possible Interval Use Time Settings Total Time 00 00 05 000 G 3 a Lou Interval Time 00 00 00 500 8 Images 10 8 Reset to Factory Settings Cance EB Fig 7 7 Index card Timelapse PE m n the index card Timelapse the settings for time controlled image capturing can be defined The time controlled image capturing is started with a click on the 1 button gt 7 2 Timelapse Time ontrolled image capturing for further information ProgRes MAC CapturePro 2 7 Release 001 39 Operational windows Setting preferences 7 1 4 1 Save images in Choose the storage place for the time controlled capturing of images Choose Folder Choose the folder for saving the images in the finder Default folder A folder will be created on your desktop with the current date as a folder name The images will be stored in this folder Show folder The folder where the images will be stored is displayed on the screen 7 1 4 2 Save images as Chooses the file format for the images to Apple Pixlet Video be store
66. s MAC CapturePro 2 7 Release 001 48 Operational windows Calibration Note Before a white shading calibration can be carried out black shading calibration has to be available 1 Remove the object slide from the light beam 2 Ensure that the displayed image is not overexposed at any loca tion The exposure in the live image can be checked by a click on the slider Exposure 3 Click White Shading to start the white shading algorithm After the successful creation of a white reference image the software will display the date of the creation of the white shading 4 Mark the checkbox Apply white shading if available if you would like to apply the white shading correction to the captured images 5 The white shading correction will be applied automatically to any newly captured images only full resolution until it is switched off by removing the checkmark from the Apply checkbox 6 If anew white shading correction is created the software will overwrite the existing file automatically SE Note A shading correction is not applied to images in bin ning mode 133 Scanner calibration The calibration of the piezo driven scanner is a requirement for micro scanning with ProgRes 14 5 ProgRes and ProgRes CF cameras With the scanner calibration the piezo electric elements that move the sensor during the micro scanning process are adjusted and the image quality is optimized The calibrat
67. s Firewire a or USB 2 0 ProgRes MAC CapturePro 2 7 Release 001 88 Calibration pattern Calibration pattern 13 89 Release 001 4 apturePro 2 7 Af C ProgRes JENOPTIK Optical Systems Business Unit Digital Imaging Goeschwitzer Str 25 07745 Jena Germany Tel 49 3641 65 2143 Fax 49 3641 65 2144 E Mail progres jenoptik com www progres camera com
68. sor Color Mono chrome Sensor resolution max Pixel size A D conversion Pixel clock Dynamic range Exposure times Analog gain ax frame rate image size mage resolution Binning Cooling Digital interface Optical connection Trigger In Out Voltage supply Power consumption Ambient conditions CT1 1 2 CMOS Color Monochrome 1280 x 1024 pixel 1 3 Mpix 5 2 um 10 Bit 48 MHz 68 dB 60 us 0 5 5 1x 8x 30 m 1280 x 1024 pixel no no USB 2 0 C Mount 0 5x TV pref GB 1 2 CMOS Color 2048 x 1536 pixel 3 15 Mpix 3 2 um 10 Bit 48 MHz 36 MHz 58 dB 50 us 5 5 1x 8x 12 fps 2048 x 1536 pixel 2x 3X no USB 2 0 FireWire a Synchronization with external devices configu rable via control software USB powered approx 2 W USB FireWire powered approx 1 9 W 2 5 W Temperature 5 55 C Humidity 5 80 96 not condensing ProgRes MAC CapturePro 2 7 Release 001 83 ProgRes camera type Stock conditions Dimensions LxWxH Weight Software External camera driver Hardware require ments ProgRes CT5 Technical specifications Gil CB Temperature 20 70 C 89 mm x 84 mm x 93 mm approx 700 g re CapturePro PC MAC TWAIN for PC only ProgRes SDK available at www progres camera com PC MS WIN 2000 XP Vista Mac OS X 10 4 or higher 3 GHz CPU 1 GB RAM 64 MB graph
69. system must be operated in accordance with the instructions Any expansions re adjustments alterations and repairs may only be carried out by persons who have been authorized by JENOPTIK The electrical installations of the room where the system is set up must be in accordance with IEC requirements Please refer to chapter 2 4 Electrical installations for the electrical installation conditions and observe the information contained in that chapter ProgRes MAC CapturePro 2 7 Release 001 10 Safety instructions Danger In the following cases the use of the camera can imply the danger of personal injury or of damage to the instrument Please do not operate the camera in the following cases e The camera is visibly damaged e camera has been stored under adverse conditions over a long period e camera has been transported under adverse conditions In these cases the camera has to be switched off and protected against unintentional use Please contact your dealer or the technical support of the manufacturer 2 2 Operating storage and transportation conditions Operating temperature X57 435 C Humidity 5 96 80 96 non condensing Storage temperature 107 450 C Please store the camera in a dry and cool place Environmental conditions ProgRes digital cameras are manufactured for the use in clean and dry environments according to EN60950 Please do not expose your ca mera to extreme environ
70. t application program for example Adobe Photoshop Choose Target When working with the TWAIN application first choose the target application for opening your captured images The finder will open where you can select your target application By a click on Open the target application will be assigned to the ProgRes MAC CapturePro application It will be started with a click on Launch target Clear Target Resets the assigned target application for AutoOpen Show Target Shows the selected target application in the finder Launch Target Starts the target application in the background of ProgRes MAC CapturePro Target to Front Brings the target application window to the front When the Command key is pressed while clicking the Capture button the image in the target application will appear in the front and can be edited Quit Target Quits the target application ProgRes MAC CapturePro 2 7 Release 001 37 Operational windows Setting preferences 7 1 2 4 Options Here the AutoExposure settings can be selected Mark the respective options to preserve the gain currently set in the image or select the automatic optimization of exposure and gain With a click on Reset any settings in the index card Acquire are reset to the factory settings 7 1 3 User interface i Image info Acquire Userinterface Time Lapse Measurement Fluorescence V Overexposure Colors c
71. t from warranty during the warranty period if the equipment is operated without observing the safety regulations In any such case JENOPTIK shall be exempt from statutory liability for accidents resulting from any operation The camera was in perfect working condition when leaving the manufacturer s premises In order to retain this standard and to avoid any risk when operating the system the user must comply with the advice and warnings contained in this manual 2 9 Safety note for use in the United States of America This device complies with Part 15 of the FCC Rules Operation is subject to the following two conditions 1 this device may not cause harmful interference and 2 this device must accept any interference received including inter ference that may cause undesired operation ProgRes MAC CapturePro 2 7 Release 001 14 Safety instructions 2 10 Disposal Special environmental protection guidelines apply when disposing the ProgRes microscope cameras Do not dispose your ProgRes micro scope camera with the usual domestic refuse Contact your dealer in case of any questions ProgRes MAC CapturePro 2 7 Release 001 15 Installation and software start 3 Installation and software start The ProgRes microscope cameras are operated using the ProgRes MAC CapturePro software For a safe installation observe the following advice Important note Before connecting the ProgRes camera to your Macintosh co
72. te which is mounted to the IEEE 1394 FireWire cable on the computer side Avoid especially any agitation of your camera during operation as the image quality may deteriorate in this case To avoid the risk of fire do not use the camera near inflammable liquids or gases 2 6 Cleaning and maintenance Cleaning the camera casing If the camera casing is slightly soiled clean it with a soft slightly moi stened piece of cloth Take care that no water enters the camera casing and gets in contact with the internal parts of the camera Do not use any aggressive substances or solvents to clean the camera Cleaning the IR filter glass The built in IR filter glass has to be protected against mechanical impact such as shock or scratching as well as against dirt Avoid fingerprints on the glass and do not touch the aperture of the C mount connection ProgRes MAC CapturePro 2 7 Release 001 13 Safety instructions Commercial detergents must not be used for cleaning the filter glass Please contact your dealer if cleaning of the filter glass should become necessary Use a brush to remove dust For the transportation and the storage of your camera please use the supplied C mount cover 2 7 Exemption from statutory liability for accidents JENOPTIK shall be exempt from statutory liability for accidents in the case of non observance of the safety regulations by any operating person 2 8 Exemption from warranty JENOPTIK shall be exemp
73. the checkbox Apply to apply the calibration to the live image White Shading Calibration Perform a white shading calibration With a click on Reset white shading the white shading calibration is can celled Mark the checkbox Apply to apply the calibration to the image Scanner calibration The scanner can be calibrated with a click on the respective button Mark the checkbox to show the calibration rectangle in the image display Information _ E Camera ProgRes 14 5 S N 0306 20 0120 Firmware 2 1 27 04 2006 a Black Shading Calibration 5 Black Shading Available 18 04 2007 Reset Black Shading Mv Apply Black Shading if Available White Shading Calibration jamie Siac ee White Shading Available 18 04 2007 Reset White Shading Apply White Shading if Available Scanner Calibration _ C 4xScnner _ Factory Calibration 9x Scanner Factory Calibration 16 x Scanner Factory Calibration C 36x Scanner factory Calibration Factory Calibration 54 Show Calibration Rectangle Cooling Mv Activate Cooling Fig 7 17 Window Calibration If a calibration has already been set Available and the date of creati on are displayed next to the Shading button ProgRes MAC CapturePro 2 7 Release 001 Operational windows Calibration 47 Operational windows Calibration Cooling Mark the checkbox to activate the cooli
74. the rectangle appears again in the dimensions which have been recently defined White balance In the software window slight deviations to the micro scopic image in white neutral colors may occur due to camera internal settings With a white balance adjustment these deviations can be compensated ProgRes MAC CapturePro 2 7 Release 001 30 Image display and acquisition Activate the pipette tool and click onto a position that depicts a neutral area in your specimen The image will be changed accordingly Please note that the areas in the image will be adjusted to a neutral color not to pure white The reset button resets the white balance to def ault a Note Before using another function of the icon bar please click the button White Balance again to deactivate the function 6 3 5 Black balance F The function White Balance changes to Black Balance in the fluorescence mode or in the black and white mode With a black balance areas in the image can be defined that are to be displayed in black RGB values 0 Use the black pipette to carry out a black balance at any location in the image If an unsuitable location in the image has been selected the black balance can be reset using the arrow 6 3 6 Change color mode CE With this button the image color can be changed to black and white or to RGB color Depending on the color mode which is currently active the button will change
75. ttings Cancel OK Fig 7 13 Index card Fluorescence n this index card the filters used for capturing multi channel fluores cence images can be activated and edited Please see chapter 7 5 Fluorescence image capturing further information Filters To add a filter click the button and insert the dye name and the emission in nm in the dialogue which opens Click OK to add the filter to the list in the table window To edit a filter double click on the filter or mark the filter and click the button Edit and change the dye name and the emission in nm in the subsequently appearing window Using the checkbox Active the filter can be activated and deacti vated The activation of a filter can also be carried out in the column Active in the display window of the filters To delete a filter mark the filter in the display window and click the button The filter will be deleted Options Here a prefix for merged images can be inserted When an image is captured using different filters the different images can be super ProgRes MAC CapturePro 2 7 Release 001 44 Operational windows Timelapse imposed and will form one image in which the respective areas are displayed in different colors chapter Eapturing 7 2 Timelapse time controlled image capturing With a click on the button Timelapse oi a window opens enabling the time controlled image capturing The settings
76. u ProgRes The items of the menu Progres are described in the following section About ProgRes MAC CapturePro amp ProgRes Jenoptik L O S GmbH 2001 2007 Application 2 5 0 b6 01 01 1904 3 2 19 02 2007 CamConJ 3 2 19 02 2007 FireCam 1 3 07 02 2007 Here information about the software versions camera drivers etc are dis played Click onto the window to hide the window Fig 5 3 About ProgRes CapturePro ProgRes MAC CapturePro 2 7 Release 001 23 Menu bar Preferences With a click on this menu item the dialogue Preferences opens This dialogue is also available with a click on the button Preferences and will be explained in chapter 7 1 Setting reference Reset to Factory Settings Resets the application settings and the camera settings to the settings preset in the factory Load Settings Loads the camera settings and the settings of the software application that have been previously saved Save Settings Saves the settings which are currently used in the software appli cation The settings can be loaded by selecting Load settings Hide ProgRes This option places the ProgRes screen in the background of your working operation Hide Others Places screens of other operations in the background Show All Shows all applications that are currently open Quit ProgRes Closes the application 5 2 The menu File Contains th

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Manual - ProgRes® MAC CapturePro 2.7