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User`s Manual - Used Lab Equipment For Sale

1. Item Qty Part Number Description 17A 1 3937900 Wiring Harness Main 115V 2 17B 1 3937901 Wiring Harness Main 230V 2 17C 1 3938000 Wiring Harness Main 3 and 4 115V 17D 1 3938000 Wiring Harness Main 3 and 4 230V 18A 1 3811500 Guardian Airflow Monitor Printed Circuit Board 18B 2 1885405 Screw Mach 6 32 x 31 PH Phil S S 19 1 3936700 Label Front Set 20A 1 3910700 Airflow Switch 20B 2 1891808 Screw Thread Cutting 6 32 x 50 PH 22A 1 1307000 Switch Rocker 2 position Blower 22B 1 1306300 Switch Rocker 3 position UV Light Only 23A 1 3904800 Front Panel 2 23B 1 3904801 Front Panel 3 23C 1 3904802 Front Panel 4 23D 1 4472400 Screw Thread cut 10 24 x 50 Type F PH Phillips 24A 1 or 2 3934500 Impeller Motorized 2 or 3 Enclosure 115V 24B 1 or 2 3934501 Impeller Motorized 2 or 3 Enclosure 230V 24C 2 3934600 Impeller Motorized 4 115V 24D 2 3934601 Impeller Motorized 4 230V 25A 1 1306900 Capacitor 2 115V 10 MFD 25B 2 1306900 Capacitor 3 115V 10 MFD 25C 2 1306600 Capacitor 4 115V 15 MFD 25D 2 1306800 Capacitor 2 230V 4 MFD effective 2MFD 25E 4 1306800 Capacitor 3 230V 4 MFD effective 2MFD 25F 2 1306800 Capacitor 4 230V 4 MFD 26A 1 1235400 Ballast Fluorescent 2 115V 26B 1 1235500 Ballast Fluorescent 2 230V 26C 1 1294000 Ballast Fluorescent 3 or 4 115V or 230V 27A 1 1235400 Ballast UV 2 or 3 115V 27B 1 1235
2. 34 Chapter 6 Maintaining Your Filtered PCR Enclosure Speed Control Light Reflector T 4 Airflow Monitor aa LED Circuit Board Airflow Switch with Adjustment Screw Silence Alarm Button Red Alert LED with Test Reset with Audible Alarm Figure 6 2 Guardian Airflow Monitor with Airflow Switch 35 Chapter 6 Maintaining Your Filtered PCR Enclosure 36 Initial Certification The Purifier Filtered PCR Enclosure has been certified at the factory for an average downflow velocity of 55 10 fpm along with the HEPA Filter Leak Test The Purifier Filtered PCR Enclosures should be certified for the proper downflow velocity required by your Safety Officer It is also a conservative recommendation to perform the HEPA Filter Leak Test again should there be any damage caused during transport Re Certification Under normal operating conditions the Purifier Filtered PCR Enclosure should be recertified at least annually The certifier should perform the minimum following tests e Downflow Velocity Test e HEPA Filter Leak Test In addition the following tests should also be performed at the user s discretion Measure of Line Voltage and Current Smoke Test to determine proper airflow patterns Lighting Intensity Test when appropriate Noise Level Test when appropriate Vibration Test when appropriate Fluorescent Light Replacement 1 Disconnect the power 2 Locate the
3. Defective electronic Replace ballast ballast UV light timer does No power Power cable to UV light timer circuit not work No lights board is disconnected Verify No display connection Replace circuit board for UV light timer if it malfunctions Airflow monitor No power Power cable to circuit board is malfunction No lights disconnected and needs to be No display connected Sensor cable needs to be connected Verify that all airflow monitor interface cables are connected Check fuses on enclosure No audible alarm Alarm has been temporarily silenced using SILENCE ALARM Chapter 8 Troubleshooting PROBLEM CAUSE CORRECTIVE ACTION Airflow Monitor Wrong alarm set Airflow monitor was not properly Malfunction point adjusted Repeat calibration steps Continued outlined in this manual in Chapter 6 Constant audible alarm Check airflow and calibration of airflow monitor See Chapter 6 Continuous alarm Check the downflow velocity of the enclosure as the airflow of the system may have changed If incorrect adjust the speed control to increase downflow velocity The HEPA filter may have become loaded If downflow velocity is correct calibrate the airflow monitor outlined in this manual in Chapter 6 Monitor alarms air way to airflow monitor sensor is Lightly clean the airway with clean air Be careful not to touch sensitive electrical components
4. 12 Not Shown Figure A 1 Replacement Parts Purifier Filtered PCR APPENDIX B DIMENSIONS See the following dimensions for the Purifier Filtered PCR Enclosures 52 Appendix B Dimensions and Exhaust Options 36 75 EXTERIOR HEIGHT 2260 INTERIOR HEIGHT 2525 INTERIOR DEPTH kk 28564 ENCLOSURE DEPTH Figure B 1 Purifier Filtered PCR Enclosure 53 PPENDIX C FILTERED PCR ENCLOSURE SPECIFICATIONS This Appendix contains technical information about all Purifier Filtered PCR Enclosures including electrical specifications environmental operating conditions and wiring diagrams e 5 Amps 115V 60 Hz or 3 Amps 230V 50 60 Hz Purifier Filtered PCR Enclosure Environmental Conditions 54 Indoor use only Maximum altitude 6562 feet 2000 meters Ambient temperature range 41 to 104 F 5 to 40 C Maximum relative humidity 80 for temperatures up to 88 F 31 C decreasing linearly to 50 relative humidity at 104 F 40 C Main supply voltage fluctuations not to exceed 10 of the nominal voltage Transient over voltages according to Installation Categories II Over voltage Categories per IEC 1010 Temporary voltage spikes on the AC input line that may be as high as 1500V for 115V models and 2500V for 230V models are allowed Used in an environment of Pollution degrees 2 1 e where normally only non conductive atmospheres are present Occasionally
5. Recommended Practice for HEPA Filters Mt Prospect IL Institute for Environmental Sciences 1986 IES Recommended Practice IES RP CC 001 96 Laminar Flow Clean Air Devices Mt Prospect IL Institute for Environmental Sciences 1986 IES Document IES CC 011 85 T A Glossary of Terms and Definitions Related to Contamination Control Mt Prospect IL Institute for Environmental Sciences 1985 ISO 14644 1 1999 Cleanrooms and Associated Controlled Environment International Organization for Standardization Contamination and Sensitivity Issues with a Real Time Universal 16S rRNA PCR Journal of Chemical Microbiology May 2000 p 1747 1752 Vol 38 No 5 Elimination of Contaminating DNA within Polymerase Chain Reaction Reagents Implications for a General Approach to Detection of Uncultured Pathogens Journal of Clinical Microbiology Mar 1993 p 646 652 Vol 31 No 3 59 60 DECLARATION OF CONFORMITY Application Council Directives 75723 E RC 89 334 EEC Standards to which conformity is declared ENG M0 ENS3022 EWS0082 Manufacturer s Name Labconeo Corporation Manufacturer s Address 8811 Prospect Avenue Kansas City MO 64132 USA Importer s Name See Shipping Customs Documents Importer s Address See Shipping Customs Documents for your equipment Type of Equipment Purifier Filtered PCR Enclosures rilier Filtered POR Enclosures T Fu Y Puriher Filtered POR Enclosungs Purtlier Filtered POR Enclosu
6. ne AN User s Manual Purifier Filtered PCR Enclosures Models 3970202 3970205 3970222 3970225 3970302 3970305 3970322 3970325 3970402 3970405 3970422 3970425 Labconco s Mascot Labby the LABster Protecting your AN laboratory environment XI LABCONCO For more information please contact us E mail sales expotechusa com Website www ExpotechUSA com Labconco Filtered PCR Enclosure Manual Warranty Labconco provides a warranty on all parts and factory workmanship The warranty includes areas of defective material and workmanship provided such defect results from normal and proper use of the equipment The warranty for all Labconco products will expire one year from date of installation or two years from date of shipment from Labconco whichever is sooner except the following e Purifier8 Delta Series Biological Safety Cabinets and PuriCare Animal Laboratory Research Enclosures carry a three year warranty from date of installation or four years from date of shipment from Labconco whichever is sooner e Carts carry a lifetime warranty e SteamScrubber and FlaskScrubber Glassware Washers carry a two year warranty from date of installation or three years from date of shipment from Labconco whichever is sooner e Glassware is not warranted from breakage when dropped or mishandled This limited warranty covers parts and labor but not transportation and insurance charges In the event of a wa
7. blocked by insects dust or debris Audible disable An alarm condition must be will not stay continuously present before the operational audible alarm can be silenced If flow conditions fluctuate near the alarm set point the airflow monitor will automatically reset it Action should be taken to bring the enclosure airflow into proper operating parameters or adjust the alarm set point lower 45 Chapter 8 Troubleshooting Service Operating Record Log Customer Name Model Number Date Installed Serial Number Unit Location Date Comments 46 APPENDIX A FILTERED PCR ENCLOSURE COMPONENTS AND REPLACEMENT PARTS The components that are available for your Filtered PCR Enclosure are listed The parts shown are the most commonly requested If other parts are required please contact Product Service 47 Appendix A Replacement Parts 48 Item Qty Part Number Description 1 1 3911000 Glass Side 2A 1 3911900 Glass Sash 2 2B 1 3911901 Glass Sash 3 2C 1 3911902 Glass Sash 4 3A 1 3938900 Sash Assembly 2 3B 1 3938901 Sash Assembly 3 3C 1 3938902 Sash Assembly 4 4A 1 3905600 Lower Air Foil 2 4B 1 3905601 Lower Air Foil 3 4C 1 3905602 Lower Air Foil 4 SA 1 39
8. impeller with housing off 5 Replace each capacitor with a new one of equal voltage and capacity 6 Reassemble each new motorized impeller by reversing the assembly steps 7 Perform a HEPA filter leak check 37 Chapter 6 Maintaining Your Filtered PCR Enclosure 2 Model Mounting Screws HEPA Filter for Motorized Motorized Impeller we Capacitor Mounted here on Electrical Subassembly located behind the Control Panel Impeller Mounting Bracket Hardware Motorized s Impellers 3 or 4 Models Figure 6 3 Motorized Impeller Replacement 38 Chapter 6 Maintaining Your Filtered PCR Enclosure Speed Control Replacement 1 Remove the bracket that the speed control is attached to See Figures 6 1 and 6 2 2 Remove the two screws holding the speed control using a Phillips screwdriver Refer to Appendix A for Replacement Parts Diagram 3 Disconnect all wires leading to the speed control Connect wires on new speed control in the same position as the old speed control 4 Reassemble to the system in the same position and with the same screws that were removed earlier 40 CHAPTER 7 ACCESSORIZING AND MobDIFYING Your FILTERED PCR ENCLOSURE There are several ways to accessorize and modify the Filtered PCR Enclosure for your individual requirements These include the addition of accessory work surfaces airflow monitors filters and storage cabinets or stands 1 Work Surf
9. w Self Closing Doors 24 ADA 9904000 9904300 24 ADA w Self Closing Doors 18 ADA 9904100 9904400 12 ADA 9904200 9904500 Base Stands 3747000 2 Telescoping Base Stand with Fixed Feet 24 w x 25 5 dx 27 5 to 33 5 h 3746701 3 Telescoping Base Stand with Fixed Feet 36 w x 29 25 dx 27 5 to 33 5 h 3746702 4 Telescoping Base Stand with Fixed Feet 48 w x 29 25 dx 27 5 to 33 5 h 3747010 2 Telescoping Base Stand with 5 Casters 24 w x 25 5 dx 27 5 to 33 5 h 3746711 3 Telescoping Base Stand with 5 Casters 36 w x 29 25 d x 27 5 to 33 5 h 3746712 4 Telescoping Base Stand with 5 Casters 48 w x 29 25 dx 27 5 to 33 5 h 42 CHAPTER 8 TROUBLESHOOTING AND SERVICER OPERATING Loc Refer to the following table if your Filtered PCR Enclosure fails to operate properly If the suggested corrective actions do not solve your problem contact Labconco for additional assistance PROBLEM CAUSE CORRECTIVE ACTION Class 100 ISO Class 5 air conditions are not present inside the enclosure Improper user techniques for the enclosure See Certifying the Enclosure Chapter 3 and Safety Precautions Chapter 4 sections in the manual Ref Appendix D External factors are disrupting the enclosure airflow patterns See Location Reguirements Chapter 2 Certify
10. 000 Table 1 1 ISO Classification Number N Polymerase Chain Reaction Definition PCR Polymerase Chain Reaction PCR is a laboratory process in which a particular DNA segment from a mixture of DNA chains is rapidly replicated producing a large readily analyzed sample of a piece of DNA In PCR DNA is immersed in a solution containing the enzyme DNA polymerase unattached nucleotide bases the subunits that DNA is composed of and primers short sequences of nucleotides designed to bind with an end of the desired DNA segment Two primers are used one primer binds at one end of the desired segment on one of the two paired DNA strands and the other primer binds at the other end but on the other strand The solution is heated to break the bonds between the strands of the DNA When the solution cools the primers bind to the separated strands and DNA polymerase quickly builds a new Chapter 1 Introduction strand by joining the free nucleotide bases to the primers When this process is repeated a strand that was formed with one primer binds to the other primer resulting in a new strand that is restricted solely to the desired segment Thus the region of DNA between the primers is selectively replicated Further repetitions of the process can produce billions of copies of a small piece of DNA in several hours PCR was developed in 1985 by Kary B Mullis who was awarded the 1993 Nobel Prize in chemistry for his work It
11. 29 Chapter 6 Maintaining Your Filtered PCR Enclosure 30 HEPA HEPA Filter Leak Test Purpose After installing the new HEPA filter the HEPA filter should be leak checked This test is performed to determine the integrity of the HEPA filter the filter housing and the filter seal Leak testing must be done by a qualified technician with calibrated equipment Remove the upper perforated diffuser located inside the enclosure by using a Phillips screwdriver to unfasten the screws See Figure 6 1 The filter passes the leak test at 01 or better Reference Leak Testing and Photometer scanning from the Institute of Environmental Services IES RP CC001 3 Apparatus 1 An aerosol photometer ATI model 2D 2E 2G or equivalent Air Techniques Hamilton Associates Inc 11403 Cron Ridge Dr Owings Mills MD 21117 2 One aerosol generator of the Laskin nozzle s type An aerosol of mineral oil or suitable liquid shall be created by flowing air through it The compressed air supplied to the generator should be adjusted to a pressure of 10 1 psig during operation Air Techniques Inc Model TDA 4A or equal One nozzle at 10 psig is 67 5 cfm x 100ug l Vol of air For the 2 at 55 fpm or 230 cfm one nozzle 10 psig is 6750 230 cfm 30 ug l For the 3 at 55 fpm or 340 cfm one nozzle 10 psig is 20 ug l For the 4 at 55 fpm or 460 cfm one nozzle at 10 psig is 15 ug l 3 Mineral oil Catalog 1491400 4 Sampling Nozzle Re
12. See Figure 4 1 and 4 2 Inherently Safe Impeller has a negative pressure plenum that surrounds the positive pressure impeller so that if a leak should occur the unfiltered air is captured and refiltered See Figure 4 2 Speed Control regulates the speed of the motorized impeller and is used by the certifier to validate and adjust the downflow velocity Located behind the front panel See Figures 4 1 and 4 2 Fluorescent Lamp is located above the work area out of contact with the clean air A safety glass window beneath the lamp distributes the light evenly across the work surface See Figure 4 1 Filter Pressure Gauge indicates the pressure across the HEPA filter to help predict HEPA filter loading See Figure 4 1 19 Chapter 4 Performance Features and Safety Precautions 11 12 13 14 15 16 17 18 19 Filter Clamping Bolt evenly seals the HEPA filter to the frame of the enclosure See Figure 4 1 and 4 2 Control Panel The control panel which is located above the sash contains the filter pressure gauge control switches and the electronics See Figure 4 1 HEPA High Efficiency Particulate Air Filter is rated to remove 99 99 of all particles 0 3 micron in size See Figure 4 2 Lamp Ballast Not Shown for the fluorescent lamp is located behind the control panel Variable Digital Timed UV Ultraviolet Lamp Not Shown allows the operator to surface disinfect the work area of the enc
13. appropriate for the application will give the quietest operation Downflow velocity measurements are made using an anemometer An electric anemometer can be obtained from your laboratory supply dealer Downflow velocity measurements should be taken 6 below the interior upper diffuser Using a small Phillips screwdriver adjust the speed control to give the required downflow velocity See Figures 6 1 and 6 2 in Chapter 6 to locate the speed control The downflow velocity is increased by turning the speed control counterclockwise and clockwise to decrease downflow velocity Validating the Filtered PCR Enclosure To determine the actual downflow velocity airflow velocity readings are taken This should be done 6 down from the upper diffuser located inside the top of the enclosure The average downflow velocity is achieved by taking readings in three rows across the enclosure with the readings 6 from the ends and evenly spaced every 6 Refer to the exhaust specifications table in Chapter 2 for proper airflow volumes and downflow velocities for your particular model Validation should be completed by a certified technician The Purifier Filtered PCR Enclosures have been tested at Labconco before shipment and provide ISO Class 5 clean air conditions inside the enclosure Labconco also performed extensive performance testing to validate the Filtered PCR s for product protection under ISO Class 5 conditions For copies of these validation
14. is used in a broad range of applications from DNA fingerprinting to medical tests to identify diseases from the infectious agent s DNA See also nucleic acid The Concise Columbia Encyclopedia is licensed from Columbia University Press Copyright 1995 by Columbia University Press All rights reserved About This Manual This manual is designed to help you learn how to install use and maintain your Filtered PCR Enclosure Chapter 1 Introduction provides a brief overview of the Filtered PCR Enclosure explains the organization of the manual and defines the typographical conventions used in the manual Chapter 2 Prerequisites explains what you need to do to prepare your site before you install the Filtered PCR Enclosure Electrical and service requirements are discussed Chapter 3 Getting Started contains the information you need to properly unpack inspect install and certify the Filtered PCR Enclosure Chapter 4 Performance Features and Safety Precautions explains how the Purifier Filtered PCR Enclosure operates and the appropriate precautions you should take when using it Chapter 5 Using Your Filtered Enclosure discusses the basic operation of how to prepare use and shut down your Filtered PCR Enclosure Chapter 6 Maintaining Your Filtered PCR Enclosure explains how to perform routine maintenance on the PCR Enclosure Chapter 7 Accessorizing Your Filtered PCR Enclosure explains acceptable modifications to the PC
15. that are preceded by the wrench icon Only a trained certifier or contractor should only perform these operations Failure to comply with the information following a wrench icon may result in injury to the user or permanent damage to your PCR Enclosure e Important information is presented in capitalized type in paragraphs that are preceded by the pointer icon It is imperative that the information contained in these paragraphs be thoroughly read and understood by the user e A number icon precedes information that is specific to a particular model of enclosure The 2 icon indicates the text is specific to the 2 foot wide model The 3 icon indicates the text is specific to the 3 foot model etc CHAPTER 2 PREREQUISITES Before you install the Filtered PCR Enclosure you need to prepare your site for installation You must be certain that the area is level and of solid construction In addition a dedicated source of electrical power should be located near the installation site to power the Filtered PCR Enclosure and other apparatus Additionally the enclosure should be strategically placed in the lab to provide efficient workflow Carefully read this chapter to learn the requirements for your installation site The support vibration and movement requirements The location and air current requirements The exhaust and blower requirements The electrical power requirements The space requirements Refer to Appendix B Dimensions
16. 07400 Bracket Air Foil R H 5B 1 3907401 Bracket Air Foil L H 6 2 1554500 Stop Sash Bumper TA 1 6916500 Latch Sash TB 1 3906700 Bracket Latch TC 1 1927403 Spring Compression Latch 7D 2 1893206 Screw 8 32 x 38 Lg Phillips Pan S S Type F 8 1 3945300 Printed Circuit Board UV Light Timer 9A 2 1889316 Screw 10 24 x 1 00 PH Phil S S 9B 2 1912108 Washer Plastic 194 ID x 380 OD 9C 2 7868402 Bushing Spacer 31 x 63 10 1 9 Ft 6913700 Wiper Sash Seal 11A 2 1936800 Bushing Heyco closed 1 50 Dia 11B 2 1934601 Bushing Heyco w Flex Shutter 1 50 Dia 12A 2 3940500 Hinge outer 12B 2 3940501 Hinge Inner 12C 4 1591606 Rubber Bumper 12D 2 3939600 2 and 4 Sash Closure Door 12E 2 3939601 3 Sash Closure Door 12F 2 3940700 Extension Bi Fold Door 3 12G 2 3940701 Extension Bi Fold Door 4 13A 1 3907700 Reflector Light 2 13B 1 3907701 Reflector Light 3 13C 1 3907702 Reflector Light 4 13D 2 1889308 Screw Mach 10 24 x 50 PH Phillips 14A 1 3708300 Lamp Fluorescent 2 Enclosure F15T8 SP35 14B 1 9721902 Lamp Fluorescent 3 Enclosure F17T8 14C 1 9721901 Lamp Fluorescent 4 Enclosure F25T8 15A 1 1270100 Lamp UV 2 or 3 Enclosure G15T8 15B 1 1271300 Lamp UV 4 enclosure G30T8 16A 1 3911700 Bracket Right Reflector Support 16B 1 3911701 Bracket Left Reflector Support 16C 4 1893108 Screw 8 x 1 2 AB Hex Washer Head Appendix A Replacement Parts
17. 500 Ballast UV 2 or 3 230V 27C 1 1233400 Ballast UV 4 115V 27D 1 1233600 Ballast UV 4 230V 28A 1 3922100 Speed Control 115V 28B 1 3922101 Speed Control 230V 29A 1 3935500 Upper Diffuser Screen 2 Internal 29B 1 3935502 Upper Diffuser Screen 3 Internal 29C 1 3935504 Upper Diffuser Screen 4 Internal 29D 1 3935501 Upper Diffuser w Air cutout 2 29E 1 3935503 Upper Diffuser w Air cutout 3 29F 1 3935505 Upper Diffuser w Air cutout 4 49 Appendix A Replacement Parts 50 Item Qty Part Number Description 30 10 1893712 Screw 10 x 75 PH Phil Thd Forming 31A 2 1881196 Capscrew 3 8 16 x 6 0 Lg Hx Hd 31B 4 1911018 Washer Flat 3 8 31C 2 1906925 Nut Hex 3 8 Nylon Lock 32A 1 3707900 HEPA Filter 2 18 x 18 x 3 32 w Gasket 32B 1 3707901 HEPA Filter 3 30 x 18 x 3 32 w Gasket 32C 1 3707902 HEPA Filter 4 42 x 18 x 3 32 w Gasket 35A 1 1333800 IEC Power Inlet 115V 35B 1 1305800 Power Cord 115V Right Angle 35C 1 1305900 Power Cord 230V Right Angle 35D l 1339200 Receptacle RFI Inlet 230V 36A 1 115V 1327204 Circuit Breaker 5 AMP 36B 2 230V 1327200 Circuit Breaker 3 AMP 40A 1 1952500 Gauge Pressure Mini Helic II 40B 1 3667400 Bracket Gauge 40C 2 1905617 Nut 10 24 KEPS 40D 2 1551700 Tubing 3 16 ID x 5 16 OD PBC 7 00 Lg 40E 2 1 Long 3788200 Snubber Filter Pressure Gauge Appendix A Replacement Parts
18. A Filter is 99 99 efficient on particles 0 3 micron Width Catalog Size 2 3707900 18 x 18 x 3 31 3 3707901 30 x 18 x 3 31 4 3707902 42 x 18 x 3 31 Prefilters Roughing A prefilter to extend HEPA filter life Filter Catalog Qty 2 3935700 1 3 3935701 1 4 3935702 1 41 Chapter 7 Accessorizing and Modifying Your Filtered PCR Enclosure 4 Storage Cabinets and Stands SOLVENT ACID 48 9902000 9901000 36 9902100 9901100 30 9902200 9901200 24 9902300 9902400 9901300 9901500 18 9901400 9901600 12 48 w Self Closing Doors 9903000 36 w Self Closing Doors 9903100 30 w Self Closing Doors 9903200 24 w Self Closing Doors 9903300 9903400 24 ADA 9906000 9906100 9905000 9905200 24 ADA w Self Closing 9906200 9906300 Doors 18 ADA 9905100 9905300 12 ADA STANDARD BASE VACUUM PUMP Size Description Dual Doors Right Hinge Left Hinge Dual Doors Right Hinge Left Hinge 48 9900000 36 9900100 30 9900200 24 9900300 9900600 18 9900400 9900700 9907000 9907100 12 9900500 9900800 48 w Self Closing Doors 36 w Self Closing Doors 30 w Self Closing Doors 24
19. A filter gasket and filter clamp bolts All seams downstream of the HEPA filter are jacketed by positive pressure This intrinsically safe design ensures Class 5 conditions and a particulate free environment for PCR experiments HEPA Filter Appropriate Use Filter Size Part No HEPA filters are high efficiency particulate 2 3707900 air filters having a particulate removable EL 3707901 efficiency of 99 99 for particles with a 4 3707902 diameter of 0 3 micron Connecting the Electrical Supply Source to the Filtered PCR Enclosure 115V Models Simply connect the 115V power cord supplied to the IEC electrical supply plug on the back of the enclosure Ifusing at 50 Hz operations blower performance maximum airflow will be reduced by 17 230V Models The 230V is shipped without a plug Install the appropriate plug for your electrical specifications per local codes Chapter 3 Getting Started Setting the Downflow Velocity with the Speed Control Adjustment of the speed control gives the correct downflow velocity and is located behind the front panel The downflow velocity should be from 30 65 fpm for Class 100 ISO Class 5 conditions ISO Class 5 clean air conditions are maximized at a setting within this range The Filtered PCR Enclosure is factory set at 55 10 fpm downflow velocity Consult your Safety Officer for airflow recommendations for your application Working at the lowest downflow velocity
20. LERS ARE NOT RESPONSIBLE FOR SHIPPING DAMAGES Do not discard the packing material until you have checked all of the components and tested the enclosure We recommend that you do not remove the enclosure from its shipping container until it is ready to be placed into its final location Move the unit by placing a flat low dolly under the shipping skid or by using a floor jack Do not move the enclosure by tilting it onto a hand truck Installing the Filtered PCR Enclosure on a Supporting Structure and Work Surface Use caution when lifting or moving the enclosure When installing the enclosure onto a chemical resistant work surface or benchtop ensure that the structure can safely support the combined weight of the enclosure and any related equipment The work surface should be at least as wide and deep as the enclosure Chapter 3 Getting Started EE MM Fr to properly support it The front of the enclosure should be aligned within 0 30 8mm of the front of the work surface Mounting holes are provided in the Labconco accessory work surfaces to secure the enclosure Work Surface Specifications The work surface should be smooth rigid and durable such as a chemical resistant epoxy resin The surface should be non porous and resistant to the materials used in conjunction with the Purifier Filtered PCR Enclosure The work surface should also contain a dished recessed area for containing primary spills Filtered PCR Encl
21. NS 52 APPENDIX C FILTERED PCR ENCLOSURE SPECIFICATIONS 54 APPENDIX D REFERENCES 59 DECLARATION OF CONFORMITY 60 CHAPTER 1 INTRODUCTION Congratulations on your purchase of a Labconco Purifier Filtered PCR Enclosure Your enclosure provides a particulate free environment for Polymerase Chain Reaction experiments It is the result of Labconco s more than 30 years experience in manufacturing filtered enclosures These enclosures will effectively provide product protection from airborne particulate matter During operation room air is drawn through the prefilter on top forced through the blower and then through the HEPA filter and diffuser The clean air in the work area in the enclosure meets or exceeds ISO Standard Class 5 conditions defined per ISO 14644 1 and Class 100 conditions as defined by Federal Standard 209E The enclosure is set to deliver nominal downward airflow of 45 65 feet per minute Each enclosure uses a single HEPA filter which is rated at least 99 99 efficient for 0 3 micron particles The Purifier Filtered PCR enclosures offer many unique features to enhance performance A variable solid state digital timer provides a UV light to destroy contaminating DNA and RNA in the work area The UV light automatically turns off to prepare for the next experiment To take full advantage of all features please acquaint yourself with this manual and keep it handy for future reference If you are unfamiliar with how Filtere
22. PA filter After the HEPA filter is replaced the enclosure MUST be certified See Figure 6 1 1 Unplug the enclosure and remove the prefilter located on top 2 Remove the front panel by loosening the two screws that secure it 3 Using a 9 16 deep socket loosen the filter clamp bolts located on top Refer to Figure 6 1 4 With the clamp bolts loosened the HEPA filter clamping frame should be clear of filter Carefully pull the filter straight out of the enclosure and discard properly 5 Cover the surface of the new HEPA filter gasket with a light coating of silicone grease if desired 6 Install the new HEPA filter by pushing it straight into the cabinet ensuring that it is correctly oriented with the gasket facing down on the exhaust side of the enclosure 7 Tighten the clamp bolts uniformly until the filter gasket is properly compressed against the frame Inspect the seal thoroughly before proceeding 8 Reinstall the front panel and prefilter 9 Plug the enclosure in and have it check for leaks and downflow velocity CAUTION The filter clamp bolts should only be tightened enough to ensure a proper seal at maximum tightness The filter gasket should be compressed 50 or less Chapter 6 Maintaining Your Filtered PCR Enclosure HEPA Filter Sealing Surface J HEPA Filter Filter Clamp Bolt Speed Control Adjustment Screw Figure 6 1 HEPA Filter Changing Diagram amp Filter Leak Test Diagram
23. R Enclosure or how to add accessories Chapter 1 Introduction G amp DU L Chapter 8 Troubleshooting contains a table of problems you may encounter while using the Filtered PCR Enclosure including probable causes and suggested corrective actions Appendix A Components and Replacement Parts contains labeled diagrams of all of the components of the Filtered PCR Enclosure Appendix B Dimensions contains comprehensive diagrams showing all of the dimensions for the Filtered PCR Enclosure Appendix C Filtered PCR Enclosure Specifications contains the electrical requirements for Filtered PCR Enclosures Wiring diagrams are also included Appendix D References lists the various resources available addressing clean work stations and PCR Enclosures Typographical Conventions Recognizing the following typographical conventions will help you understand and use this manual e Book chapter and section titles are shown in italic type e g Chapter 3 Getting Started e Steps required to perform a task are presented in a numbered format e Comments located in the margins provide suggestions reminders and references e Critical information is presented in boldface type in paragraphs that are preceded by the exclamation icon Failure to comply with the information following an exclamation icon may result in injury to the user or permanent damage to the enclosure e Critical information is presented in boldface type in paragraphs
24. able digital timed UV light and UV sash closure 15 Chapter 4 Performance Features and Safety Precautions Airflow Switch with Adjustment Screw E DS Figure 4 2 Filtered PCR Enclosure Airflow Diagram Chapter 4 Performance Features and Safety Precautions Figure 4 3 UV Sash Closure 18 10 Chapter 4 Performance Features and Safety Precautions Aerodynamic Lower Air Foil allows air to sweep the work surface and promote ISO Class 5 air conditions inside the enclosure See Figure 4 1 Ergonomic Slope of 10 degrees provides maximum visibility and comfort reduces glare thereby minimizing operator fatigue See Figure 4 1 Internal Depth of 25 provides necessary depth to work inside the enclosure See Figure 4 1 Safety Glass Sash with Spring Loaded Latch has a wiping seal and features a spring loaded latch to secure sash open for loading and cleaning The sash must be down for normal operation See Figure 4 1 Utility Ports with Iris allows electrical cords and data cords to pass through the back of enclosure without leaving a large hole The enclosure ships with solid plugs Iris plugs are included with the User s Manual See Figure 4 1 and 4 2 Optional Guardian Airflow Monitor continuously monitors airflow An audio visual alarm alerts the user to low airflow conditions The Guardian Airflow Monitor is an available option on all Purifier Filtered PCR Enclosure models
25. aces An optional dished work surface is available to attach to the Filtered PCR Enclosure Dished work surfaces are contoured to fit the dimensions of the filtered enclosures to contain spills Epoxy is chemical resistant See Figure 3 1 for installation Catalog Description Dimensions W x D x H 3909900 Black 2 foot wide 24 x 29 x 1 3909901 Black 3 foot wide 36 x 29 x 1 3909902 Black 4 foot wide 48 x 29 x 1 3909903 Gray 2 foot wide 24 x 29 x 1 3909904 Gray 3 foot wide 36 x 29 x 1 3909905 Gray 4 foot wide 48 x 29 x 1 Chapter 7 Accessorizing and Modifying Your Filtered PCR Enclosure 2 Guardian Airflow Monitor The Guardian Airflow Monitor allows you to continuously monitor airflow through the enclosure The Guardian monitor can be placed on any Purifier Filtered PCR Enclosure Description Order Part s Individual Parts Required Guardian Airflow Monitor 115V or 230V 1 ea 3811500 Airflow Monitor Printed Circuit Board l ea 3910700 Airflow Sensor w Connector 2 ea 1885405 6 32 x 31 Phillips machine screw to mount Airflow Monitor Circuit Board 2 ea 6 32 x 50 Phillips thread cutting screw to 1891808 E mount Airflow Sensor lea 3935501 Upper Diffuser w Air cutout 2 lea 3935503 Upper Diffuser w Air cutout 3 lea 3935505 Upper Diffuser w Air cutout 4 3 Accessory Filters HEPA Filter HEP
26. ctangular 1 2 x 3 1 4 Air Techniques Inc Procedure For the ATI 2G Photometer 1 Turn on the photometer and allow it to operate for a minimum of 5 minutes Leave the valve in the CLEAR setting 2 Press the ENTER keypad Press the REF keypad 3 The display will display P1 for approximately 1 second and then display a numerical value 10 11 Chapter 6 Maintaining Your Filtered PCR Enclosure Using the or v keypads respectively increase or decrease the numerical value until it equals 30 2 20 3 15 4 for the Purifier Filtered PCR enclosure at 55 fpm downflow velocity Press the ENTER Keypad The photometer will scan for 15 seconds and then the 0 keypad will flash Press the Enter keypad The unit will scan for 5 seconds the display will read 0000 and the unit will sound a confirming tone Set the valve to DOWNSTREAM Place the palm of your hand over the sampling port of the pistol There should be a strong vacuum at this port If the vacuum is weak contact Air Techniques Hamilton Associates Turn the enclosure on and let it operate for a minimum of 5 minutes If necessary adjust the speed control of the enclosure to maintain the following downflow airflows at 55 fpm 2 230cfm 3 340 cfm 4 460 cfm Position the aerosol generator discharge in the intake of the prefilter on top of the enclosure Start the aerosol g
27. d PCR Enclosures operate please review Chapter 4 Performance Features and Safety Precautions before you begin working Even if you are an experienced user please review Chapter 5 Using Your Filtered PCR Enclosure which describes the enclosure features Chapter 1 Introduction ISO Class 5 Definition Airborne particulate cleanliness inside any PCR Enclosure is designated by ISO Class 5 which is equivalent to 3520 particles 0 5 um or larger per cubic meter of air per ISO Standard 14644 1 ISO Class 5 cleanliness is illustrated in the table to follow and is equivalent to Class 100 air conditions as defined by Federal Standard 209E Class 100 is equal to 100 particles 0 5 um or larger per cubic foot of air Table 1 1 Selected airborne particulate cleanliness classes for cleanrooms and clean zones ISO Maximum concentration limits particles m3 of air for particles equal to and classification larger than the considered sizes shown below concentration limits are number N calculated in accordance with 3 2 of Standard 14644 1 0 1 um 0 2 um 0 3 um 0 5 um 1 um 5 um ISO Class 1 10 2 ISO Class 2 100 24 10 4 ISO Class 3 1 000 237 102 35 8 ISO Class 4 10 000 2 370 1 020 352 83 ISO Class 5 100 000 23 700 10 200 3 520 832 29 ISO Class 6 1000000 237000 102 000 35 200 8 320 293 ISO Class 7 352 000 83 200 2 930 ISO Class 8 3 520 000 832 000 29 300 ISO Class 9 35 200 000 8 320 000 293
28. d re measure the downflow velocity to confirm 5 Replace the front panel and tighten the screws Calibrate and Operate the Airflow Monitor Optional Guardian Airflow Monitor Refer to Figure 6 2 for operation and calibration Labconco Airflow Monitor Airflow Switch Operation The Guardian Airflow Monitor consists of a circuit board and an airflow switch This switch indicates airflow as safe or low It does not provide an actual velocity but a small setscrew in the back of the sensor can adjust the airflow level that it classifies as good safe or low alert The circuit board provides power to the sensor and also contains a safe green and alert red airflow LED indicators as well as a SILENCE ALARM button to quiet the audio alarm When first powered up the Printed Circuit Board PCB will light both red and green LED indicators and sound the alarm to indicate it is working After 5 seconds the air monitor will indicate either good or bad airflow based on what the connected airflow switch detects For low airflow the unit will wait for 10 seconds after low indications before it sounds both the audio alarm and the red alert LED indicator If Chapter 6 Maintaining Your Filtered PCR Enclosure the SILENCE ALARM button is pressed the audio alarm will be silenced but the red alert LED will remain on The alarm is silenced indefinitely unless an airflow change is detected If safe airf
29. ed PCR Enclosure provides a continuous downflow of particulate free HEPA filtered air Data for the outflow exhaust volume downflow velocities and out flow face velocities are listed for each Filtered PCR model below mh nnn amp cv e Chapter 2 Prereguisites aa r En Out Average Flow Outflow Downflow Exhaust Noise Face Enclosure Velocity Volume Pressure Velocity Width Model Description fpm CFM db A fpm 2 2 Filtered PCR Enclosure 30 120 33 91 85 40 165 58 60 115 50 210 60 61 145 60 250 62 63 170 3 3 Filtered PCR Enclosure 30 180 58 60 85 40 250 60 62 115 50 310 61 63 145 60 370 63 54 170 4 4 Filtered PCR Enclosure 30 240 60 62 85 40 330 62 64 115 50 420 66 69 145 60 500 68 70 170 Electrical Requirements Standard duplex electrical receptacles should be nearby for connecting the Filtered PCR Enclosure or other equipment The enclosure includes iris pass throughs to allow electrical cords through the back of the enclosure without leaving a large hole Space Requirements The dimensions for all the different models are shown in Appendix B Dimensions CHAPTER 3 GETTING STARTED Now that the site for your Filtered PCR Enclosure is properly prepared you are ready to unpack inspect install and validate your enclosure Read this chapter to learn how to Unpack and move the enclosure Set up the enclosure with the proper supporting structure and work sur
30. enerator Pressure to be 10 1 PSIG Ensure that one Laskin nozzle is in the open position Allow the generator to operate for a minimum of 15 seconds Scan the downstream exhaust side of the HEPA filter by passing the sampling nozzle of the gun in slightly overlapping strokes over the entire surface the filter with the sampling port not more than 1 inch from the surface of the filter media Scan the entire periphery of the filter and the gasket between the filter frame and the enclosure frame Scanning shall be done at a traverse rate of not more than 2 inches per second Acceptance Aerosol penetration shall not exceed 0 01 percent measured by the photometer 31 Chapter 6 Maintaining Your Filtered PCR Enclosure 32 Setting the Downflow Velocity with the Speed Control Adjustment 1 Remove the front panel by loosening the 2 Phillips screws on top that secure the front panel 2 The speed control is located on the electrical subassembly located behind the switched control panel and below the front panel See Figure 6 1 3 Adjust the speed control with a small Phillips screwdriver by turning the screw counterclockwise to increase blower speed or clockwise to decrease the blower speed The speed control is very sensitive so proceed with caution 4 Measure the downflow velocity per the averaging technique outlined in Chapter 3 and adjust the speed control slowly for the desired speed Allow the speed to stabilize an
31. face Verify HEPA filters integrity Connect the electrical supply Set the downflow velocity with the speed control adjustment Validate enclosure airflow Seal the enclosure to the work surface Depending upon which model you are installing you may need common mechanical and electrical installation tools in addition to wrenches ratchets sockets a nut driver set a flat blade screwdriver a Phillips screwdriver and a carpenter level to complete the instructions in the chapter Each enclosure model weighs between 125 to 195 N Ibs each 55 to 85 kg The shipping container allows for lifting with a mechanical lift truck or floor jack If you must lift the enclosure manually follow safe lifting guidelines Do not lift by the lower air foil Chapter 3 Getting Started Unpacking the Enclosure The United States Carefully remove the shrink wrap or carton on the enclosure and Interst t Commerce inspect it for damage that may have occurred in transit If Commission rules damaged notify the delivery carrier immediately and retain the require that claims be entire shipment intact for inspection by the carrier filed with the delivery carrier within fifteen 15 days of delivery UNAUTHORIZED RETURNS WILL NOT BE ACCEPTED IF ENCLOSURE WAS DAMAGED IN TRANSIT YOU MUST FILE A CLAIM DIRECTLY WITH DO NOT RETURN GOODS WITHOUT THE PRIOR AUTHORIZATION OF LABCONCO THE FREIGHT CARRIER LABCONCO CORPORATION AND ITS DEA
32. for complete enclosure dimensions Refer to Appendix C Filtered PCR Enclosure Specifications for complete filtered enclosure electrical and environmental conditions specifications and requirements Chapter 2 Prerequisites Support Vibration and Movement Requirements At a minimum the supporting structure usually consists of a base cabinet or base stand and chemically resistant work surface Location and Air Current Requirements The Purifier Filtered PCR Enclosures have been designed to provide particulate free air meeting ISO Class 5 conditions by negating typical cross drafts and turbulence within the opening However as a precautionary safety measure and a higher level of quality management it is recommended that the Filtered PCR Enclosure be placed in an area away from e High traffic areas where walking might cause an air disturbance or be a nuisance e Overhead or wall HVAC diffusers fans radiators or other lab equipment producing air currents e Doorways or windows that may be opened Airflow Specifications The Purifier Filtered PCR Enclosures use an integral motorized impeller to draw room air from the top and through the enclosure This room air is then pushed down through the HEPA filter The enclosure is factory set to deliver 45 65 fpm downflow velocity measured 6 below the upper diffuser The Class 5 HEPA filtered air flows across the work area and exits out the front and back of the enclosure The Filter
33. however a temporary conductivity caused by condensation must be expected in accordance with IEC 664 Appendix C Filtered Enclosure Specifications Appendix C Filtered Enclosure Specifications ain fir ata Zu MDK ae 145 Za BR a WTS tee NVAOVIC OMIM OONOOSV1 MIE 4 Bie i L FOS 93809 ADH IMS h Ms pre en nn nn nn ee eee CERA md ALA wild eY A PRICE al FUMA KAR 56 Appendix C Filtered Enclosure Specifications Eki te esit MATE WALA AAS MEHIA OINOIEY I Luna V ADILI B BIKE Y HDA MP1 RIE ai roe A Wi DALE mfg DA Ba Aa R e 20 E ar 2 T Jo da a To a En E e di 57 Appendix C Filtered Enclosure Specifications MAA mii ue AN 4 da CET we x G r STK nga i Ei Dir G ae w Ki te ma W HOWIO busi d ot va Rn eee 2 i q a 4 4 4 4 4 4 i 1 1 i 4 1 1 1 4 a 4 a i i H i 1 1 1 1 i 58 APPENDIX D REFERENCES Many excellent reference texts and booklets are currently available The following is a brief listing Bryan D and R C Marback 1984 Laminar airflow equipment certification What the Pharmacist needs to Know American Journal of Hospital Pharmacy 41 1343 1348 General Services Administration 1988 FED STD 209E Federal Standard Clean Room and Work Station Requirements Controlled Environment Washington D C IES Recommended Practice IES RP CC 001 86
34. ical Requirements Space Requirements CHAPTER 3 GETTING STARTED Unpacking Your Enclosure Installing the Filtered PCR Enclosure on a Supporting Structure and Work Surface Verifying HEPA Filter Integrity Connecting the Electrical Supply Source to the Filtered PCR Enclosures Setting the Downflow Velocity with Speed Control Validating the Filtered PCR Enclosure Sealing the Filtered PCR Enclosure to the Work Surface CHAPTER 4 PERFORMANCE FEATURES AND SAFETY PRECAUTIONS Components Safety Precautions CHAPTER 5 APPROPRIATE APPLICATIONS FOR YOUR FILTERED PCRT ENCLOSURE Routine Daily Work Procedures Suitable Applications HEPA Filter Applications Suitability amp Guidelines CHAPTER 6 MAINTAINING YOUR FILTERED PCR ENCLOSURE Routine Maintenance Schedule When to Replace HEPA Filters How to Install a New HEPA Filter BRWNN FR 44 G G G L 12 13 13 14 15 16 20 23 23 24 25 26 2 Zi 28 HEPA Filter Leak Test 30 Setting the Downflow Velocity with the Speed Control Adjustment32 Calibrate and Operate the Airflow Monitor 32 Initial Certification 36 Re Certification 36 Fluorescent Light Replacement 36 UV Light Replacement 37 Motorized Impeller Replacement 37 Speed Control Replacement 39 CHAPTER 7 ACCESSORIZING amp MODIFYING YOUR FILTERED PCR ENCLOSURE 40 CHAPTER 8 TROUBLESHOOTING amp SERVICER OPERATING LOG 43 APPENDIX A FILTERED PCR ENCLOSURE COMPONENTS AND REPLACEMENT PARTS 47 APPENDIX B DIMENSIO
35. iences Particulates are filtered by the HEPA filter before air is returned to the room Biohazardous materials or other hazardous solids cannot be used with the Purifier Filtered PCR Enclosure as air is drawn over the work surface and out of the enclosure The Purifier Filtered PCR Enclosure only provides product protection and offers no personnel protection whatsoever Chapter 5 Using Your Filtered PCR Enclosure and Appropriate Application Listed below are suitable applications for HEPA filters HEPA Filter Applications Salta btt and Guidelines Procedures traditionally performed on an open bench where a clean Class 5 air environment is now required e The HEPA filtered enclosure provides product protection from particulate matter Because air from the enclosure enters the room during operation this enclosure should only be used for operations requiring sample protection from environmental contamination e HEPA filters are only effective for entrapment of particulate matter The surface of the HEPA filter is fragile and should not be touched Care must be taken to avoid puncturing the HEPA filter during installation If you suspect that a HEPA filter has been damaged DO NOT use the enclosure See Chapter 6 for Replacing the HEPA Filter e The HEPA filter will gradually accumulate airborne particulate matter from the room The rate of accumulation will depend upon the cleanliness of the room air and the amount of time the enclo
36. ing the Enclosure Chapter 3 and Safety Precautions Chapter 4 sections of this manual Ref Appendix D Enclosure has improper downflow velocity Have enclosure certified and check exhaust system Check HEPA filters for loading Adjust enclosure speed control Enclosure should have an average downflow velocity of 30 65 fpm Blower won t operate Unit not plugged into outlet Plug the enclosure into appropriate electrical service 43 Chapter 8 Troubleshooting 44 PROBLEM CAUSE CORRECTIVE ACTION Blower won t Circuit breaker s Reset circuit breaker operate or Ground Fault Interrupter Blower wiring is Inspect blower wiring disconnected Blower switch is Replace switch defective Motorized impeller or blower is defective Replace motorized impeller or blower See Chapter 6 Poor internal Class 5 air conditions Enclosure sash not closed Close sash HEPA filter Replace HEPA filter or increase speed clogged Blower and lights Unit not plugged Plug enclosure into appropriate won t operate into outlet electrical service Circuit breaker s tripped Reset or replace circuit breaker Lights do not work Lamp not installed Inspect lamp installation properly Lamp wiring Inspect lamp wiring disconnected Defective lamp Replace lamp Light switch is Replace light switch defective
37. ler or change the HEPA filter when the downflow velocity falls below acceptable limits established by your Safety Officer Ensure only trained operators use the enclosure New users should review the User s Manual and become familiar with the operation of the enclosure Proper operation of the enclosure depends largely upon the enclosure location and the operator s work habits The enclosure should be located away from traffic patterns doors fans ventilation registers fume hoods and any other air handling device that could disrupt its airflow patterns Consult Chapter 2 Prerequisites and Chapter 3 Getting Started sections of this manual for further details CHAPTER 5 APPROPRIATE APPLICATIONS FOR YOUR FILTERED PCR ENCLOSURE Now that the installation of your Filtered PCR Enclosure is completed you are ready to use it Read this chapter to learn about 1 Routine Daily Work Procedures 2 Suitable Applications 3 Appropriate HEPA Filter Applications Suitability and Guidelines Routine Daily Work Procedures Planning e Arrange for minimal disruptions such as room traffic or entry into the room while the enclosure is in use Start up e Turn on blower and light Turn off the UV light e Only raise the sash for loading and cleaning e Allow the enclosure s blower to operate unobstructed for 1 minute 23 Chapter 5 Using Your Filtered PCR Enclosure and Appropriate Application 24 e Wear a long sleeved lab c
38. losure when it is not in use The UV light rays will not penetrate the plane of the sash and lower airfoil The UV light timer is variable with eight settings in minutes of 5 10 15 30 60 120 240 and infinity This provides extreme flexibility for PCR protocol It is easily changed by selecting the time necessary located on the control panel To operate simply select the UV light option on the light switch UV Sash Closure provides added safety to prevent access inside the enclosure See Figure 4 3 Accessory Work Surface is dished and contoured to fit the dimensions of the enclosures to contain spills Upper Diffuser Screen provides protection for the HEPA Filter and promotes even distribution of downflow air See Figure 4 2 Prefilter roughing enhances the life of the HEPA filter by trapping large particles It should be replaced quarterly Safety Precautions l 20 Although the enclosure has been engineered to maintain Class 5 air conditions caution should always be used while working Prior to using the enclosure check to make 10 11 12 13 Chapter 4 Performance Features and Safety Precautions sure that the blower is operating and periodically ensure that air is flowing through the enclosure at its specified downflow velocity of 30 to 65 fpm The use of an airflow monitor is recommended to alert the user to a problem with airflow Use good housekeeping in the enclosure at all times Clean up spil
39. low is later detected for 10 seconds the green safe LED will be lit and the alert red LED will be shut off At any time the airflow is safe good one can press the SILENCE ALARM test button and the audio alarm and the red LED will turn on as long as this button is held down The PCB has also a two pin connector for use as an external output with isolated relay contacts that close when the red alert LED is lit low airflow These relay contacts are not affected by the SILENCE ALARM button The PCB is mounted behind the front panel using standoffs and an appropriate label is used to highlight the SILENCE ALARM button with clear areas for the red and green LED s By special order the PCB can be prepared as a factory special with an additional connector for the following external inputs and having the following possible functions e External Alarm allows an external signal to sound the alarm from the building airflow system of a failed condition e Alarm Disable allows an external signal to prevent a low airflow alarm from occurring e Night Setback allows an external signal to prevent a low airflow alarm from occurring not any different from Alarm Disable above other than the terminology e Contact Labconco for ordering information on this special PCB Calibration 1 Ensure the flow switch and alarm circuit board are installed and operational 2 Allow the enclosure to operate for at least two mi
40. ls immediately Periodically clean enclosure interior Do not overload the work surface with apparatus or work material The Purifier Filtered PCR Enclosures should never be used with biohazardous materials toxins acids or radionuclides Air from the work area is dispersed directly into the laboratory from the enclosure A qualified Safety Officer must carefully assess any risk associated with the use of the Purifier Filtered PCR Enclosure Always work with your hands as far back into the enclosure as possible Keep all materials and apparatus inside the lower air foil of the enclosure Do not work in this enclosure without the blower running Perchloric acid use in this enclosure is prohibited Radioactive materials are prohibited in this enclosure Consult your Safety Officer Avoid cross drafts and limit traffic in front of the enclosure Air disturbances created may disturb Class 5 conditions A qualified certification technician should test the enclosure before it is initially used The enclosure should be validated annually and whenever it is relocated The sash must remain in the down position while using the enclosure The HEPA filter provides Class 5 clean air conditions and only provides product protection inside the enclosure Do not use the enclosure for personnel protection Never use flammable gases or solvents in the enclosure Use of an open flame must be avoided in the enclosure Open flames may disrupt
41. nutes 3 If factory installed the monitor will alarm at 30 10 fpm downflow velocity with the average outflow velocity set at 85 10 fpm 4 To change the factory setting set the downflow velocity to the desired alarm condition using the speed control adjustment procedure outlined in Chapter 6 Once the alarm condition is set use a small screwdriver to turn the adjustment screw on the airflow switch counterclockwise facing the screw until the low airflow red LED lights and the audible flow alarm sounds 5 Adjust the downflow velocity to the nominal operating point required The green safe LED should light 33 ee een eee nee SS AAA AAA 20 Chapter 6 Maintaining Your Filtered PCR Enclosure 6 Over time the HEPA filter will load and eventually slow the downflow velocity Once the alarm condition is met simply increase the speed control as outlined in Chapter 6 or replace the HEPA filter if the speed control is maximized 7 The table below lists typical alarm conditions based on normal operating conditions Typical alarm conditions are set at downflow velocities of 10 to 30 feet per minute below the normal operating conditions due to supply air and exhaust air fluctuations as well as room air cross drafts Consult your Safety Officer or Laboratory Manager for proper operating speeds Enclosure Operating Alarm Condition Set Point Downflow Speed Speed 55 10 fpm 25 35 fpm 45 10 fpm 25 35 fpm
42. oat rubber gloves and use protective eyewear Wear a protective mask if appropriate Consult your Safety Officer for additional personal protective equipment recommendations e Wipe down interior surfaces with a mild household detergent or disinfectant DO NOT use abrasive cleaners bleach or solvents Loading Materials and Equipment e Load only the materials required for the procedure Do not overload the enclosure Do not obstruct upper air diffuser screen e Large objects should not be placed close together After loading wait one minute to purge airborne contaminants from the work area Work Techniques e Keep all materials inside the lower air foil and perform all operations as far to the rear of the work area as possible e Segregate all clean and contaminated materials in the work area Final Purging e Upon completion of work the enclosure should be allowed to operate for two to three minutes undisturbed to purge airborne contaminants from the work area before shutting down the blower e Select the UV time for your PCR protocol and then turn on the UV light to decontaminate the work area as determined by your Safety Officer Unloading Materials and Equipment e All open trays weigh vessels or containers should be covered before being removed from the enclosure Suitable Applications Suitable applications for the Purifier Filtered PCR Enclosures are based on guidelines established by the Institute for Environmental Sc
43. osure Work Surface Installation 1 Level the base cabinet or stand and the work surface See Figure 3 1 2 Position the work surface in its intended location and with the front of the work surface towards you Rear mounting holes are located close to the rear edge 3 Secure the work surface to the base cabinet or stand with a structural adhesive or silicone sealant 4 Insert the supplied mounting screws in the four holes Allow a minimum of 1 8 clearance under the head of the screw for positioning the enclosure 5 Place the enclosure on the work surface and slide the rear flange and front air foil flanges under the mounting screw heads 6 Tighten the four screws to complete the installation 10 Chapter 3 Getting Started INFLOW AIR dEr EXTERIOR H 25 64 EXTERIOR DEPTH P if home i 25250 2 INTERIOR DEPTH E EXHAUST pr 2900 DEPTH ICP TIOMALS oo WORKSURFACE WORKSURFACE HEIGHT Figure 3 1 Filtered PCR Enclosure Installation 11 Product Service 1 800 522 7658 Chapter 3 Getting Started 12 Verifying HEPA Filter Integrity The HEPA filter is shipped installed with the gasket on the downstream side The HEPA filter is leak checked at Labconco A second leak check is recommended before using the enclosure and at least annually thereafter Consult your Safety Officer and Chapter 6 for the HEPA Filter Leak Test See Figure 4 2 for HEPA filter location HEP
44. reports contact Labconco Customer Service 13 Chapter 3 Getting Started Sealing the Filtered PCR Enclosure to the Work Surface When the Filtered PCR Enclosure has been set in place and wired it may be sealed at the work surface to prevent materials from collecting under the walls A bead of silicone sealant is recommended to seal the Filtered PCR Enclosure to the work surface 14 CHAPTER 4 PERFORMANCE FEATURES AND SAFETY PRECAUTIONS The Purifier Filtered PCR Enclosure is designed to provide particulate free protection from airborne matter During operation room air is drawn from the top and filtered by the HEPA filter providing Class 5 conditions inside the enclosure The Purifier Filtered PCR Enclosure delivers Class 5 air conditions when operating at downflow velocities of 30 to 65 feet per minute The HEPA filter is jacketed by negative pressure preventing contaminated air inside the enclosure and ensuring Class 5 air conditions inside the enclosure After each Polymerase Chain Reaction PCR experiment the variable timed UV light can be run to decontaminate the work area from DNA and RNA The Purifier Filtered PCR Enclosure HEPA filter is located behind the front panel Users are encouraged to routinely check airflow to satisfy Class 5 air conditions inside the enclosure or purchase a model with an airflow monitor For additional surface decontamination inside the enclosure users are encouraged to use the vari
45. res 4970200 Serios Serial No Various See Individual Declaration Year of Manufacture 2004 and subsequent 1 the undersigned hereby declare that the equipment specified above conforms to the above Directiveds and Standardis See individual Declaration of Conformity which will be signed by the importer for your country Signature Full Name Position An individual version of this declaration is included with your shipping customs documentation Labconco P N 16960 533 REV For more information please contact us E mail sales expotechusa com Website www ExpotechUSA com
46. rranty claim contact Labconco Corporation or the dealer who sold you the product If the cause is determined to be a manufacturing fault the dealer or Labconco Corporation will repair or replace all defective parts to restore the unit to operation Under no circumstances shall Labconco Corporation be liable for indirect consequential or special damages of any kind This statement may be altered by a specific published amendment No individual has authorization to alter the provisions of this warranty policy or its amendments Lamps and filters are not covered by this warranty Damage due to corrosion or accidental breakage is also not covered Limitation of Liability The disposal and or emission of substances used in connection with this equipment may be governed by various federal state or local regulations All users of this equipment are required to become familiar with any regulations that apply in the user s area concerning the dumping of waste materials in or upon water land or air and to comply with such regulations Labconco Corporation is held harmless with respect to user s compliance with such regulations Part 3938200 Rev ECO B731 TABLE Or CONTENTS CHAPTER 1 INTRODUCTION ISO Class 5 Definition Polymerase Chain Reaction Definition About This Manual Typographical Conventions CHAPTER 2 PREREQUISITES Support Vibration amp Movement Requirements Location and Air Current Requirements Airflow Specifications Electr
47. small light reflector located under the control panel 3 Remove the light reflector by removing two Phillips screws on the bottom of the light reflector 4 Rotate and remove the old fluorescent lamp 5 Reinstall the new fluorescent lamp and light reflector in reverse order 6 Power the unit up and try the new fluorescent lamp Chapter 6 Maintaining Your Filtered PCR Enclosure UV Light Replacement 1 Disconnect the power Locate the UV lamp located inside the enclosure Rotate and remove the old UV lamp Reinstall the new UV lamp Power the unit up and try the new UV lamp pide eS Motorized Impeller Replacement The motorized impeller must be replaced as a complete unit When the motorized impeller is replaced the capacitor should also be replaced See Appendix A for Replacement Parts Diagram See Figure 6 3 for an isometric view of the motorized impeller plenum assembly The HEPA filter rests below the motorized impeller assembly 1 Unplug the enclosure from the electrical outlet 2 Remove HEPA filter per the HEPA filter removal procedure outlined earlier in this chapter 3 Consult the wiring diagram in Appendix C of the manual and disconnect all the wires of the motorized impeller Be sure to connect wires on the new motor in the same way the old motor was wired The 3 and 4 enclosures have two motorized impellers 4 Remove four screws in each motorized impeller WARNING High speed blower Never operate
48. sure is operating With normal usage the HEPA filters will last two to five years before requiring replacement 26 CHAPTER 6 MAINTAINING YouR FILTERED PCR ENCLOSURE Monitoring airflow and changing the filters is the primary maintenance required Certification and recertification is also reviewed in Chapter 6 Review this chapter on maintenance for the following Routine Maintenance Determination of when to replace the HEPA filters How to install a new HEPA filter HEPA filter leak test Speed control adjustment and setting the downflow velocity Operating and calibrating the airflow monitors Initial certification Re certification Fluorescent light replacement 10 UV light replacement if applicable 11 Motorized impeller replacement 12 Speed control replacement vsonaupunn Chapter 6 Maintaining Your Filtered PCR Enclosure Routine Maintenance Schedule Weekly Wipe down the interior surfaces of the enclosure with a disinfectant or cleaner depending upon the usage of the unit and allow to dry Using a damp cloth clean the exterior surfaces of the enclosure particularly the front and top to remove any accumulated dust Operate the exhaust system noting the airflow velocity out the front of the enclosure using a source of visible smoke Airflow monitors are recommended for constant monitoring Monthly or more often as required Determine the actual downflow velocity 6 below the upper diff
49. the airflow patterns in the enclosure burn the HEPA filter and damage the filter s adhesive Gases under high pressure should not be used in the enclosure as they may disrupt airflow patterns 21 Chapter 4 Performance Features and Safety Precautions 22 14 15 16 17 18 19 20 21 HEPA filters are only effective for entrapment of particulate matter The surface of the HEPA filter is fragile and should not be touched Care must be taken to avoid puncturing the HEPA filter during maintenance If you suspect that a HEPA filter has been damaged DO NOT use the enclosure contact a local certification agency or Labconco The HEPA filter will gradually accumulate airborne particulate matter from the room The rate of accumulation will depend upon the cleanliness of the room air and the amount of time the enclosure is operating With normal usage the HEPA filters will last two to five years before requiring replacement Avoid direct exposure to ultraviolet UV radiation NEVER work in the enclosure when the UV light is on Ensure that the enclosure is connected to an electrical service in accordance with local and national electrical codes Failure to do so may create a fire or electrical hazard Do not remove or service any electrical components without first disconnecting the filtered enclosure from electrical service Increase the downflow velocity by adjusting the speed control of the motorized impel
50. user of the enclosure where the average reading should be at the specified velocity Use calibrated thermal anemometer or other approved apparatus Airflow alarms are recommended for constant monitoring All weekly activities Check downflow velocity Increase speed control or change HEPA filter when downflow velocity of the enclosure drops below the recommended speed for your facility or if the airflow alarm monitor alerts you An airflow monitor is recommended Annually Replace UV lamps if equipped Have the enclosure validated by a qualified certification technician See Certification and Recertification to follow in Chapter 6 All monthly activities Change prefilter When to Replace HEPA Filters The HEPA filter in the Purifier Filtered PCR Enclosure gradually accumulates airborne particulate matter from the room The rate of accumulation will depend upon the cleanliness of the room air and the amount of operational time the enclosure is operating In typical installations and usage the HEPA filters will last two to five years before requiring replacement Replace HEPA filters 27 Chapter 6 Maintaining Your Filtered PCR Enclosure 28 when downflow velocity drops below the recommended 30 65 fpm velocity and the speed control is adjusted to full speed Replace HEPA filter if it fails the HEPA Filter Leak Test in Chapter 6 How to Install a New HEPA Filter NOTE Only a qualified certifier should service the HE

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