pH Meter Manual.indd
Contents
1. Select C F In the Main menu go to crit ig and press Enter press Enter iia Press the Up or Down arrow to MA between C and F then press Enter to confirm the selection Power saving options The meter can switch off automatically after a preset time to conserve battery life e In the Main menu go to e Go to BS ae e Go to Fo saving ont ior and press Enter e Go to iet sr and ee plea The display shows Sita GIN time of meter Omir i ig and press Enter press Enter again e Use the Up or Down je to set the ON time from 1 minute to 55 minutes or Continuous no automatic power off e Press Enter to return to the Power saving menu 12 RI PH Meter System OPTIONS Select language The meter can be set to one of four languages English German French or Spanish e To change the language in the Main menu go to fst ig and press Enter e Goto Parame press Enter again Goto eieact ia Use the Up or Down key to go to the desired language and press Enter and press Enter e Goto Hack and press Enter to return to the Main menu Calibration buffers The buffers supplied by RI are set by default Other buffersets can be selected e g DIN NIST but we do not recommend changing from the RI buffers mV millivolt reading The ISFET mV output can be directly displayed If the meter is not yet calibrated the actual ISFET mV output will be shown If the meter has been ca2. a situation occurs that requires the user to take action indicated by the situation and the action to be taken Display pH and Temperature The pH value can be shown with either one or two decimal resolution depending on the calibration method and the meter setting The meter measures the sample s temperature with a sensor built into the probe and provides automatic temperature compensation for an accurate pH reading The temperature can be shown on the display The system offers four display settings pH 0 1 without temperature indication pH 0 1 with temperature indication pH 0 01 without temperature indication pH 0 01 with temperature indication To change between these settings go to the Main menu Use the Arrow keys to go to fii st and press Enter repeatedly until the desired setting appears Note with a 1 point calibration the accuracy is not sufficient for a 0 01 pH indication When selecting the 0 01 pH resolution indication after a 1 point calibration the second decimal will blink to indicate that it is not to be regarded as accurate We therefore advise performing a 1 point calibration for 0 1 pH accuracy or a 2 point calibration for 0 01 pH accuracy RI PH Meter CALIBRATION The system must be calibrated before first use using correct buffers and for maximum accuracy the probe and the buffers must be at the same temperature as the sample i e 37C The pH Meter has four built in calibration tables
3. each corresponding to a different set of buffers The RI bufferset is selected by default We recommend using a two point calibration using only RI buffers and bufferset which have been optimised for IVF needs The system automatically stores calibration results for later review See page 14 Make sure that the buffers used during calibration are identical to the selected bufferset otherwise significant variations in measurement values may occur Over time the pH value of a buffer may change Buffers with values over pH 7 00 in particular are susceptible to CO contamination To maintain the accuracy of calibration make sure that the buffers used are fresh and not contaminated by other materials Research Instruments provides buffers in small bottles specially designed to facilitate this use When being used the bottle should be opened for the shortest possible time With minimal exposure to the air each bottle should last for at least three months Before calibrating Ifthe probe is newly connected to the meter indicated by status text line or if the meter has been switched off for several holes it is necessary to place buffer solution in the probe with the meter switched on for a period of 10 minutes This allows the probe to set itself for use after a period of inactivity and is required to ensure stable readings Rinse the probe before calibration During calibration the 1 indication will blink in the display I
4. lying at the bottom of the container e Allow to stand for two hours to allow remaining particles to settle and decant the clear solution into a clean container 23 RI PH Meter WARRANTY This RI meter is produced packed and shipped with the utmost care If defects do arise this RI meter is warranted to be free from defects in material and craftsmanship for the period of 12 months Any defect due to misuse of the product is not covered by the warranty All RI probes have a 6 month limited warranty please refer to the probe manual for specifics RI disclaims any liability to customers to users of its products or to any other person or persons for any special or consequential damage that might arise out of or that might in any way be connected with the use of this instrument or its accessories The warranty described in this paragraph shall be in lieu of any other warranty expressed or implied including but not limited to any implied warranty or merchantability or fitness for a particular purpose The buyer s sole and exclusive remedy is for repair or replacement of defective parts as provided therein Representations and warranties made by any person including dealers representatives and employees of RI which are inconsistent or in conflict with the terms of this warranty shall not be binding upon RI unless in writing and signed by one of its officers RI reserves the right to ask for proof of purchase such as the
5. original invoice or packing slip There are no serviceable or replaceable parts in this product Do not remove any covers as this could damage the instrument and invalidate the warranty 24
6. probe that has been in use for some time Probe still gives accurate results but may require cleaning Use water toothbrush and a mild detergent as described on page 22 to ensure optimum performance then recalibrate If the slope remains low use fresh buffers and recalibrate If the slope is close to 90 and does not improve the probe may be aging and a replacement should be purchased 18 RI PH Meter aa Ed TROUBLESHOOTING In high humidity levels drops in temperature may cause problems due to condensation EMC Immunity When using the AC adapter some interference may be observed at EN50082 1 limits If unstable readings are observed which cannot be related to the sample the condition of the probe or meter try to obtain stable reading on a different location by RF shielding the sample or by disconnecting the AC adapter Reset If the display contrast has been adjusted to an unreadable setting it may be necessary to reset the meter Other unforeseen circumstances that hamper the meter s functioning may also require a meter reset e Press and hold the Power key for 40 seconds The meter may be seen to switch on and off a number of times After releasing the Power key the meter will restart automatically within 5 seconds It may be necessary to reset the display contrast see page 11 All other data and parameters are automatically saved and restored by the system Recalibrating the system is recomm
7. 105 If it is below this please refer to the trouble shooting guide on page 18 10 RI PH Meter System OPTIONS Autoread Stability Function The Autoread function provides a clear indication that the measured pH value is stable e Inthe Main menu go to The RE ed will briefly SHON AulLoread is ON The amp symbol is permanently shown in the ect see page 6 e Pressing Enter once more es he Autoread unono The display will briefly show ji and the AF symbol is no longer shown in the ede When the Autoread function is on the amp F symbol and the pH value will blink if the measured pH is Paaa at more than 0 02 pH per 30 seconds The AR symbol and pH value will blink for the first 8 seconds as the meter calculates the initial stability Display contrast The display contrast can be adjusted to suit the ambient light conditions e Inthe Main menu go to Eef iz and press Enter Go to L K and press Enter The status text line will show ont rast Use the Up or Down key to change the display contrast setting e When you are happy with the contrast press Enter to confirm the setting and return to the Configuration menu e Goto Note it is possible to change the display contrast to a setting where it is very difficult to read the display at all Reverse the display setting immediately to a readable contrast ck and press Enter to return to the Main menu 11 RI PH Meter System OPTIONS
8. RI PH Meter DH Meter User Manual Issue 1 2 2nd January 2007 RESEARCH INSTRUMENTS LIMITED Bickland Industrial Park Falmouth Cornwall TR11 4TA U K Tel 44 0 1326 372 753 Fax 44 0 1326 378 783 E mail sales research instruments com website http www research instruments com RI PH Meter CONTENTS eie ie ie AAR OO RE a eee 2 Declaration of Conformity sk EE esse is Ne NE eek Sie Ed see 3 Bey ie ME AE EE AE N N OE 4 BI MR RE OE Or 5 Connections Battery EER DEER Ee SE DE GE EG EE 7 Sie de AE AA EE OE EE EEE 9 System Options AUtoread ER iEn 11 Display Contrast ab De ee ee 11 Select C F RE AE EET ER ER 12 Power Saving ia RAN SR ene ea aas ee 12 Select Language EER KEER EREGAS NE ER Ee ee 13 Calibration Buffers sa REDE NE EE Ere 13 Millivolt reading sessies seine Ee ERG NEE Ke ie ee 13 Calibration Data Storage n 14 Troubleshooting sussie EER Ee Re NE Ke EER ee Ge RE ee 15 RESOL sd ER EA Ge EE es Ed nt 19 Specifications EE EE GE aiia ka iia aa 20 Default Settings riria EE N N 21 Cleaning Procedure xeiiecisatieceninireniaiaardicontatectanenthicnnxalennnmaseece 22 Warranty cneas EE RE a eee E EE Ea 24 RI PH Meter aa INTRODUCTION Caution text here This symbol with italic text next to it denotes information that is essential to avoid damage to the equipment or to embryos this denotes messages displayed on the screen Text like this denotes a button to be pressed on the meter Please rea
9. andles priorities to make sure that only one ATT code is shown at a time No probe signal Ensure that the probe is properly The pH is out of the 0 14 range The sample reading is more than 3 pH units out of the calibrated range The pH is out of the 0 14 range during calibration The calibration points are less than 0 5 pH units apart connected to the meter Ensure that there is a good contact between the sample and the probe Clean the probe page 22 Verify the buffer and the buffer recognition during calibration The probe may not have been calibrated to the meter recalibrate The probe may be contaminated clean the probe and recalibrate For the best results the expected pH value should be between the calibration buffers Recalibrate with a wider calibration range or use more calibration points Check that the probe is properly filled with the buffer Clean the probe see page 22 and recalibrate When the calibration points are too close together accurate slope calculation is difficult Check the buffers and use other buffers if necessary Check the buffer recognition during calibration RI aa ad TROUBLESHOOTING The calibration points are more than 7 pH units apart The ISFET output is too close to the ISFET output in the previous buffer The probe signal is not stable There is no thermistor signal The temperature is out of the probe s working rang
10. correct buffer recognition during calibration Check that the buffers used match the programmed bufferset Clean the probe see page 22 and recalibrate Recalibrate using fresh buffers Check for correct buffer recognition during calibration Check that the buffers used match the programmed bufferset Clean the probe see page 22 and recalibrate Check times used for Power Saving See page 12 Reset the meter See page 19 Occurrence of Reset the meter See page 19 strange pixels 17 RI aa PH Meter TROUBLESHOOTING General No display Battery may be completely discharged Connect meter to AC power and wait 10 minutes before turning on the meter Contrast may be set to extreme value Reverse contrast setting to readable value See page 11 If unable to find contrast setting menu reset the meter See page 19 Calibration slope values Slope Interpretation 98 0 105 0 Probe gives maximum performance 94 0 97 9 90 0 93 9 Probe functions fine Some maintenance cleaning with water toothbrush and a mild detergent as described on page 22 can bring it up to maximum performance If such low slopes are achieved by new probes it is advised to place hand warm water in the probe for 20 minutes and then buffer pH 4 00 for 1 2 hours This will effectively remove any KCI crystallisation that may have occurred in the reference diaphragm due to long storage Typical for a
11. d this manual and the Quickstart guide carefully and follow the instructions to ensure that the pH meter system will work reliably for a long time All wetted materials used are in compliance with the demands of the Food amp Drug Administration FDA of the United States of America A There are no serviceable orreplaceable parts in this product j Do notremove any covers as this can damage the instrument and compromise warranty The information in this manual is current at the time of publication Our commitment to product improvement requires that we reserve the right to change equipment procedures and specifications at any time In no event does Research Instruments assume the liability for any technical or editorial errors or for any damages arising therefrom This manual is protected by copyright all rights reserved and no part hereof may be photocopied or reproduced in any form without the prior written consent of Research Instruments If your pH meter has this label and you are located in Europe it means that if the meter is no longer serviceable it must be sent back to RI to be destroyed in an environmentally safe way Never dispose of the meter with normal waste 2 RI PH Meter aa dd EC DECLARATION OF CONFORMITY Application of the Council Directive EMC Directive 89 336 EEC Equipment type Industrial and scientific equipment Unit trade name pH Meter amp pH Meter Datalogging Harmonized Euro
12. e There is no signal from the 3 resistor PH Meter When the calibration points are too far apart the results are less reliable Check the buffer recognition during calibration Recalibrate using fresh buffers Check that the correct buffer is in the probe Recalibrate using fresh buffers If this does not help replace the probe Allow 10 minutes stabilisation before calibration Make sure there is good contact between probe and buffer Stir buffer well before reading Clean the probe see page 22 and recalibrate Without this signal temperature compensation ATC is not possible Check the probe is properly connected to the meter If this does not help replace the probe The probe may be damaged Check the sample temperature Internal probe malfunction Check the probe is properly connected to the meter Replace the probe 16 RI aa ad TROUBLESHOOTING General The meter detects a new probe The slope is too low slope less than 90 The slope is too high slope over 110 Probe status is too low Meter unresponsive PH Meter Perform a new calibration with this probe The sensitivity of the sensor is too low Recalibrate using fresh buffers Check for correct buffer recognition during calibration Check that the buffers used match the programmed bufferset Clean the probe see page 22 and recalibrate The sensitivity of the sensor is too high Check for
13. e If two blocks are shown by new probes it is advised to fill the probe cavity with warm water for 20 minutes and then in buffer pH 4 for 1 2 hours then recalibrate One block is typical for a probe that has been in use for some time The probe still gives accurate results but may require cleaning see pade x Recalibrate using fresh buffers If none of the recommended remedial actions lead to probe status improvement the probe is near the end of its life and will need to be replaced in the near future RI PH Meter DISPLAY pH 7 0 pH value indication These large digits represent the pH value Temperature indication Temperature in C or F Note this is not intended to give an accurate reading For an accurate reading we advise purchasing an RI IVF Thermometer Low Battery status indication When the remaining operational time is less than approximately 5 hours a low indication will appear When charging the battery two alternating symbols are shown on the right hand side of the battery symbol Autoread activated When the Autoread function automatic stability check is activated F symbol will be shown continuously thus providing a clear visual stability indication i 41 Calibration indication In the bottom left corner the text i will be blinking when calibration is in progress This is the menu for changing the meter settings Use the Arrow keys to highlight the desired section
14. ended A prolonged exhausted battery may result in an automatic meter reset Recharge the battery promptly when the battery status shows Low 19 RI SPECIFICATIONS Measuring range Resolution Accuracy Calibration Buffer tables Environment Power supply Battery charging time Dimensions incl crad PH Meter pH 0 00 to 14 00 Temperature OC to 60C pH 0 1 or 0 01 pH user selectable Temperature 0 1C pH 0 01 Least Significant Digit LSD using 2 or more point calibration 0 1 using 1 point calibration Temperature 0 5 C 1 or 2 point calibration with automatic buffer recognition Sets of DIN NIST and JIS buffers a set of 5 RI supplied NIST traceable buffers Temperature Measurement OC to 40C Storage 5C to 70C Rel Humidity Measurement 85 Storage 95 AC Mains adaptor with NiMH rechargeable battery backup 24 hours when empty with meter off le Length 215 mm Height 60 mm Width 95 mm Weight 325 gram tion for the pH reading but is otherwise not to Not guaranteed The temperature indication is sufficiently accurate to ensure good temperature compensa be regarded as being accurate For accurate incubator temperature monitoring we recommend a calibrated thermometer such as the RI IVF Thermometer 20 RI PH Meter DEFAULT SETTINGS Following table shows the minimum and maximum values and the factory set default value for various pa
15. f a calibration has not been successful a message to that effect will be shown on the display Also the calibration procedure can be cancelled before it is finished In both cases the meter will use data from the last successful calibration 9 RI PH Meter CALIBRATION Performing the calibration In the Main menu go to 3 1 it and press Enter On the status indication line the selected bufferset is shown Fill the probe cavity with buffer 7 00 viret j ei and then F ai iis died If the buffer value recognised is not 7 00 go to st and press Enter repeatedly until the correct buffer value is shown on the display then return to ai i With ai i highlighted press Enter to confirm the selection Leave mig buffer i in the probe for two indie pe ad snows fer and after some time i is highlighted Rinse the probe cavity with deionised water and fill with buffer 10 00 Menu iem Check the display shows F iA If not go to st and press Enter EE y until the correct buffer value is shown on the display then return to ai 2 With 3 i highlighted press Enter to confirm the selection Leave the buffer in the probe for two minutes After completing the calibration the slope percentage is briefly displayed and the meter automatically returns to the Main menu The slope data is automatically recorded for later use as described on page 14 The slope value displayed should be between 98 0 and
16. librated the corrected mV output related to the value at pH 7 00 is shown e In the Main menu go to iq and press Enter e Go toFarams press Enter again e Go to Ti The display shows The mV value reading remains active press Enter to ren to the Configuration menu 13 RI PH Meter System OPTIONS Calibration data storage After the meter is calibrated all calibration data are automatically stored in the meter s memory and can be recalled Information stored includes the bufferset used buffer value s temperature slope s and the probe status Data can be recalled on the screen e In the Main menu go to amp and press Enter e Go toFarams press Enter again Gotolalibration data The meter will display the data from the last calibration as shown e Go tof and e Enter to display the previous calibration data set ee to ext and press Enter to display the next data set The calibration data is shown consisting of the probe status and the chosen bufferset The buffer value and temperature are shown as well as the calculated slope 14 RI PH Meter aa ad TROUBLESHOOTING ATT Attention Codes The pH meter has builtin diagnostic functions allowing itto automatically detect problems and display an error message The display will show amp T Ts where xxx is a number indicating the nature of the Sef If there is more than one error the pH meter itself h
17. oak and rinse described above This ensures that particles are removed before brushing Most scratches occur when there are hard particles on the ISFET that are brushed against the surface This causes scratches on the surface of the chip and damages the epoxy around the chip which can give bad readings FLUSH BEFORE BRUSH 22 RI PH Meter CLEANING PROCEDURE Refreshing the probe After cleaning it is necessary to refresh the probe This process charges the membrane with the ions needed to ensure correct operation of the probe Fill the probe cavity with saturated KCI potassium chloride solution at room temperature for at least 30 minutes Storage When not in use the probe should be stored correctly to avoid the need for lengthy refreshing procedures e Rinse the probe cavity with distilled deionised water The probe is supplied with a plastic cap with a fibrous plug Apply a few drops of distilled deionised water to the fibrous plug and gently push the cap onto the probe ne ey ey A d Pe bi aN Ed ai km sd S eg i ow Do not fit the cap with the cavity filled with liquid as the d resulting pressure may force liquid through the membrane d and damage the probe Preparing potassium chloride solution Add potassium chloride granules to distilled deionised water and stir until dissolved e Continue adding granules until no more is dissolved and undissolved granules are
18. pean standards which have been applied Standard Test laboratory reference EN50081 1 Emission standard DARE the Netherlands Report 99C00620 EN50082 1 Immunity standard DARE the Netherlands Report 99C00620 David Lansdowne Technical Director Research Instruments Ltd RI PH Meter CONTROLS The RI pH meter is controlled by the six keys located on the keypad The names Power Enter and Arrow keys Left Right Up and Down will be used consistently throughout this manual pe Enter Up arrow Left arrow Right arrow Down arrow 05 Power WV Figure 1 After starting the meter by pressing Power the display will briefly show F i ris the meter type RI pH meter the software r revision number and date and the meter s serial number Then the Main menu will be shown As the system is not yet calibrated the pH value shown in the display is not reliable RI PH Meter DISPLAY Display The pH value can be shown in 1 or 2 decimal resolution and temperature can be shown or be omitted In chapter 3 these settings are explained in detail The display contrast can be set over a wide range allowing easy reading under virtually any ambient light conditions page 11 var FT Probe Status Indicator Three blocks means that the probe gives maximum performance Two blocks means that the probe is working well Some maintenance cleaning procedure page 21 can bring it up to maximum performanc
19. rameters Parameter saving Continuous Continuous C On Off se RI DIN NIST JIS RI Contrast 2 cf _ pH Resolution Temperature Temperature English German French Spanish oO oO 21 RI PH Meter CLEANING PROCEDURE In many cases of probes that are returned as faulty the cause is contamination from the fluid that the user is measuring If the probe is not cleaned regularly the diaphragm can become blocked In this case there is no electrical contact between the electrode and the ISFET chip and the probe will not work To prevent this the probe must be cleaned regularly We recommend cleaning once a week or if the diaphragm is no longer white The probe must be refreshed page 23 and then calibrated page 9 after cleaning Cleaning Fill the probe cavity with MP Biomedicals 7X detergent solution in distilled deionised water at a temperature of 60C and leave for at least 5 minutes Rinse with deionised or distilled water Clean the inside of the cavity with a soft toothbrush and rinse again After measurements in proteins we advise trying each of the following in turn in this order 1 Put buffer 10 and buffer 3 alternately into the probe cavity then rinse with water 2 Put 4 NaOH solution into the probe cavity This must be at room temperature and for the minimum necessary time Scratches To avoid scratches on the chip epoxy clean with a brush only after the detergent s
20. then use the Enter key to enter the submenu 6 RI PH Meter CONNECTIONS Connect the probe to this connector at the bottom of the meter It will only fit in one orientation Tighten the screw collar by hand to secure Connect the mains power supply adaptor to this connector The meter s batteries are charged automatically when the mains adaptor is connected Battery The battery is a rechargeable Nickel Metal Hydride NiMH type The battery is fully charged before shipment but we recommend connecting to mains power for at least 12 hours before relying only on battery power A fully charged battery will typically give 24 hours of use We advise recharging the battery as soon as the low battery indicator appears on the display Overcharging the battery is not possible and the supplied NiMH batteries do not suffer from the so called memory effect 7 RI PH Meter DISPLAY A prolonged exhausted battery may result in an automatic meter reset After recharging a completely exhausted battery a reset may be required to restart the meter see page 18 First connect the mains adaptor and then switch off the meter This allows a visual check that the battery is charging correctly indicated by the signs next to the battery symbol Help function The pH meter is equipped with an onboard help function designed to provide instant assistance according to the task being carried out If
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