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1. A 11121 I 3DNVH 4000 aDI 31x3 1 12 0620061 97 2 2 INOOLTIS 381 TIdKVS PLE 381 TANS Gr l OzOvvc amp JANI LIINI 202 2 38nl LIPNI 77 I 9 S1OZL TANIA LIINI D 1 San LIMNI Er ASSY JIVJ YANNI 1 10 000S026 27 l ATENJSSY 7874 TONINOJ INOAJ 1 14 8268 17 I ATANASSY dva 401831 3 1 10 00010 2 Ov Aker TANYd ADVE OLE S 002 0LE BE I NOSNAS JANLYAJAJL OLE E1062 BE LE ATEWJSSY 33131 SOOO JINNI 1 S1 EEE006 SE 188558 NOOO 401831 3 1 20 2012852 SE I A Peer AJADI dili 1 91 8060061 vE DvB8 aDIB31X3 I 1E 0E00861 EE INA 370 4 31v1d SONY X000 SSY TD YANNI 1 91 28 100811 22 0N01 9 41815 NOTAJL SNIT 1403 951081 12 135549 34021715 SOOO 33NNI OLE 801801 OE 3401715 133529 AVJABLVJH OLE 201801 62 1815 HOII S 0 JINNI O O ve BEEOOBI BE Via INOAJ 818008 ZZ ATANASSY SOOO YANNI 70 008 92 3371038 SNIB L 1 917 920078 52 pl 831113 VId3H 378ISUdSIO 100022 yc E 8 AJHSVM WAS 34021715 QU c 1 9800 EZ ze gf NU MI 8700 22 2 UD OF X 01 882 3AVM 5006 ie E AJHSYM 1774 55 DI pel 9500 02 E N SNIMOI JU X3H SS ZE 8 640019 6 I SaITIIHA CH SSML 8 1 1 X ZE 8 ssooss 18 B ABUS SdITIIHd OH SSH3L X E 8 PGDOSS B 83435 HB NOTAN 2 1 X OZ v 1 060066 l Wan dH4 2 1 x Oy y 92
2. ATGWASSY NS 1N3N0400211 61 2160061 8 v 1301001 NOTA 86006 L 8 13835 SWIS 1X4 dHd SS X 0 460066 9 v 432145 SS 1876 X VIO v 906 6 4 091 1V1SONU1H UM Y 3821 1 5 611007 WN22XS WI 3513 61067 4 22 YS 38141 021062 WN 22 X S 3018 351141 601067 0 1914 44 87076 6 138V1 3 03 4 666024 9 081108 OdJIM YOLVENINT 531438 OLE 099161 9171 X 1 9N13Y38 NOTA 870021 9 310045 381M NOTA 1801 S dild SS 8 8 X Ov r 10066 v LAN X3H HSVIMI dZ 9006 t LAN XAH HSVMWI dZ 9 20062 M382S dHd v X 9 61024 1 A10 0114 82530 18Y ON 13Y4 STYI4JLYN JO 1118 3133 0008 OL NOWNOD 03007 9r9 9r9 10 22 10 6SLE NI S SLAN 4112 660019 7 030834 NO1 909 MOM LO v0 10 8SLE NI r 1 310N 0300Y N011979197990 06 90 90 NI SUJOVAS 780616 Y 0300Y Na1 904 9r9 z0 s2 60 8v0 NI Z 260066 OL 62082 9 06122 WOU 042 NOT dd WOM 10 62 011 pGBZ NI NOILONGOYd 804 03511130 440 1820 G90 1078276 WN 0 NO1 1A38 40 NOI1dIU2S30 dadd OY2 48 30 ON N23 38 2766 IPSE OVSE 966 OL NONNO2 8 9 002 01 U38NDN Ou 8 7 Direct Heat C02 Incubator ientific Thermo Sc Section 8 Spare Parts 1 002 8652 EXC cw SATINY OSLSy DIHU Y L318YR 578 X08 3321430 3ALLISNSS 211V1S03 98 SNUL
3. GA COLOR GA COLOR 6 BROWN 21 18 RED 42 22 3 BLACK 2 6 BLUE 22 18 GREE 43 22 3 RED 3 6 saV YEL 23 8 ORANGE 22 3 t R EN 4 6 BLACK 1124 IB GREE 5 6 WHITE 125 22 BLUE 45 22 BLACK B 8 BLACK 26 22 BLUE 40 20 RED 7 6 YELLOW 27 22 BROW 47 20 RED 8 8 ORANGE 28 22 BROW 48 20 BLUE 8 RE 29 22 YeLLow 4 20 BLUE 0 8 BLUE 30 22 Tim 90 20 ORANGE 1 18 RED 31 22 RED 51 20 ORANGE 2 32 22 52 20 BLACK 3 a pine 33 22 GREE 53 18 BROWN 4 zi PY 34 22 GRAY 54 24 41 BLACK 5 om PE 35 22 PURPLE 55 24 4 WHITE 6 1 36 22 ORANGE 55 24 BLACK 7 18 ORANGE 37 2273 BLAC 37 24 4 RED 8 18 BLACK 38 22 3 RED 58 2474 GREEN 9 18 YELLOW 39 2273 WHITE 1 59 20 1 40 22 2 RED 60 4 22 2 BLACK MODEL DESCRIPTION 370 STERI CYCLE T C CO2 INCUBATOR 380 STERI CYCLE I R CO2 INCUBATOR 3598 STERI CYCLE T C 02 INCUBATOR 3599 STERI CYCLE I R CO2 INCUBATOR 3584 STERI CYCLE T C CO2 INCUBATOR RH DISPLAY 3585 STERI CYCLE I R CO2 INCUBATOR RH DISPLAY 3540 STERI CYCLE T C CO2 INCUBATOR 115 VOLT 3542 STERI CYCLE I R CO2 INCUBATOR 115 VOLT 10 JIN 4592 10 15 14 DAG SAG CCS 360283 WAS 360230 9 14 4460 02 26 14 GLM SAG DRP 1250138 WAS 250085 EI 10866 12 14 12 GLS GLS LON REVISED RH SENSOR CONNECTION 7 IN 3845 2 24 10 GJG
4. m Section 1 Installation ans Start Up Locate the opening in the top left corner of the interior chamber Remove the tape from the opening on the outside of the unit Locate the stopper with filter in the hardware bag Lift the metal port cover and install the assembly in the opening inside the chamber See Figure 1 17 Also in the hardware bag is the CO sensor cover plate Install using the 1 4 turn fasteners Refer to Figure 1 17 for the location s Thumbscrews 1 STO HEPA CO Sensor Filter Cover Plate Access Port Filter Ar Sample and stopper Filter assembly J Figure 1 17 Filter and Plate Remove the filter from the shipping bag 2 Install the air sample filter assembly to the black hose barb behind the top duct Insert the other end of the filter assembly onto the metal tubing on the top duct Refer to the Figure 1 18 behind top duct Air Sample Filter Figure 1 18 Air Sample Filter Installation Direct Heat C02 Incubator 1 9 Section 1 Installation and Start Up HEPA Filter Installation Leveling the Unit Electrical Power Connection 1 10 Direct Heat C02 Incubator Caution Be careful when the filter The media can be damaged if it is mishandled To avoid damage to the incubator do no
5. Digital LED 0 196 increments Direct Heat C02 Incubator Specifications are based on a nominal voltage of 115V or 230V in ambients of 22 C to 25 C 7 1 Section 7 Specifications 7 2 Direct Heat C02 Incubator Shelves Dimensions 18 5 x 18 5 47cm x 47cm Construction Stainless steel belt sanded both sides Surface area 2 4 sq ft 0 22 sq m per shelf Max per chamber 38 4 sq ft 3 6 sq m Loading 35 lbs 16kg slide in and out 50 lbs 23kg stationary Standard r lunas cta a xo s 4 eed bod M o a a 16 Construction Interior volume 6 5 cu ft 184 liter Interior Type 304 stainless steel shiny finish Extenoresetco oo Bae 4 18 gauge cold roll steel Outer door gasket Four sided molded magnetic Santoprene Inner door gasket Bulb silicone Insulation Mineral wool Fittings Access port 1 1 4 32mm removable silicone plug CO len na rotes 1 4 6 4mm barbed Sample port Front mounted barbed Electrical 115 Volt models 275 115VAC 50 60 Hz 1PH 9 6 FLA Operating range 90 125VAC 230 Volt models 230VAC 50 60 Hz 1 4 4 FLA Operating Range 180 250VAC Power switch circuit breaker 2 Pole 12 0 Amp Accessory outlet Voltage equal to the cabinet input 75 Watts maximum 0 5ma leakage current Alarm contacts Dev
6. p p u se oJgreorjdde Jr OO pue say ojdures sre yi 130d 555256 yqq Vedy POUBUJJUTEJN 22 101eqnour IYI IONUL IY 39 yursi q UONFIGIJED 355 o georjdde se uonedqipeo pue 3 QD 1 pur AIU3A doi 7 ued Zirprumnu ou ur YoY Teas Joyse3 100p pue sasury 100p 32edsug S A JULI 200 IPAD preo x SUyJUOJN 901 ppuo A A Aea uonoy uon s z y yl 10 HQ0009 S L 10 aAnuaAaJd Thermo Scientific Section5 Routine Maintenance Warning If the unit has been in service turn it off and disconnect the power cord connector before proceeding with any maintenance A Caution Before using any cleaning method except those recommended by the manufacturer users must check with the manufacturer that the proposed method will not damage the equipment A Use an appropriate disinfectant All articles and surfaces must be thoroughly cleaned rinsed with sterile water and rough dried Warning Alcohol even a 7066 solution is volatile and flammable Use it only in a well ventilated area that is free from open flame If any component is cleaned with alcohol do not expose the component to open flame or other possible hazard Do not spray the T C sen
7. 1 1 Keypad Op ration r uu a oda bester tan reta Lad 1 3 Displays aan sesto eta Maa 1 4 Installing the Incubator 1 4 Stacking the Incubators 1 5 Preliminary Cleaning s k iya sts bala tono K Preje VE odeje 1 6 Duct Sheets and Shelf Installation 1 7 Access Port Filter amp CO2 Sensor Cover Plate 1 9 Air Sample Filter Installation 1 9 HEPA Filter Installation 1 10 Leveling the EE es 1 10 Electrical Power Connection 1 10 Filling the Humidity Pan 1 11 Connecting the CO2 Gas Supply 1 12 Incubator Start Up ass a A Da alee Aa 1 13 Calibration oe ar xta aS kus Oa cia ero aD ante ae 2 1 Temperature pana s tate bite ek RR RUR 2 1 Thermal Conductivity CO2 System 2 2 Infrared CO System auo eese dicet qu m RS I PAD Ss 2 3 Relative Humidity aae oues caa eae tes cu 2 4 Direct Heat C02 Incubator vi Table of Contents Section 3 Configuration 3 1 Turn Audible Alarm ON OFF 3 1 New HEPA EE 3 1 Set REPLACE HEPA filter reminder 3 2 Set Access Code escas
8. Meek bb EE 3 2 Enable Low Temp Alarm to Trip Contacts 3 3 Set Low Temp Alarm Limit tracking alarm 3 3 Set High CO2 Alarm Limit tracking alarm 3 4 Set Low CO2 Alarm Limit tracking alarm 3 4 Set New Zero Number for T C CO2 Sensors 3 5 Enable CO2 Alarms to Trip Contacts 3 5 Setting a Low RH Alarm Limit 3 6 Set New Span Number for T C CO2 Sensors 3 6 Enabling Temp RH to be Displayed 3 7 Enable RH Alarms to Trip Contacts 3 7 Section 4 e EE 4 1 Sensor Fault Alarms ai sete G9 cc Cot rear ac ea oa 4 2 Temp Controller Failure 4 2 EMP CNTR ERR Ae a i 4 2 Section 5 Routine Maintenance 5 1 Clean Cabinet Exterior 5 2 Replace Power Fuses 5 3 HEPA Filter Maintenance 5 3 Clean Glass Doors uras aa VR NE AA 5 3 Clean Humidity Pas ooa eR uem EA 5 3 Replace Air Sample Filter s z a eR nd ege EN 5 4 Electronics Section accum dte deve qx vacan reae x AM ad 5 4 Sterilization Cycle 5 6 Sterilization Vele osa qoe aar ten ee Er NUR REA Ea 5 8 vii Direct Heat C02 Incubator Thermo Scien
9. Series 8000DH C0 Incubator Direct Heat with Sterilization Cycle Operating and Maintenance Manuals 7013540 Rev 4 Visit us online to register your warranty Thermo www thermoscientific com labwarranty SCIENTIFIC Preface Model Sensor Voltage 3540 T C 115 3541 T C 230 3542 IR 115 3543 IR 230 T C is a thermal conductivity sensor IR is an infra red sensor All units are 50 60 Hz MANUAL NUMBER 7013540 4 31343 IN 4592 10 22 14 New magnetic door switch parts list expl dwg elec schematics RoHS CCS 3 29174 IN 4469 3 5 14 Updated solenoid pgs 8 5 amp 9 1 through 9 3 CCS 2 30384 IN 4435 1 7 14 Added SRO ext door part number 1900567 to parts list CCS 1 24851 24849 IN 3971 1 7 10 02 valve connection inlet amp inner door switch kits parts list amp drawing 370 200 5 ccs 0 25694 IN 3954 10 26 09 Release level 2 added spring rod kit pg 1 7 CCS REV ECR ECN DATE DESCRIPTION By Thermo Scientific Direct Heat C02 Incubator i Preface CAUTION Contains Parts and Assemblies r aA Susceptible to Damage by Electrostatic Discharge ESD Important Read this instruction manual Failure to read understand and follow the instructions in this manual may result in damage to the unit injury to operating personnel and poor equipment performance A Caution All internal adjustments and maintenance must be performed by qualified service personnel A Material in this manual is for information p
10. 1160061 1600061 3171 v 831113 143 JI 100011 431113 VI 006 1109 11425 SHL SS B E X 22 01 L 900069 LOINN H 9101 2 WOVE Q3HSnug 82 111H8 N GLIP 133H 1900 F M uv1000 621061 OLON SI9SI 9418 0 340 10101 LAN X3H SS 8 01084 INN X3H 0c v 0062 0114182530 Lav ON 1474 S1VIS3IVN JO 927006 0300Y 3 310N 03007 OT 1900084 803 3jSV3139 a ps 10 22 0 651 r feo oe rol vere z KE EPSE ZHGE 1796 OSE 966 OL NOWNOD 8 3 Direct Heat C02 Incubator Thermo Scientific e FXXX OSLSy OIHO YLLJIYYW 679 XOB 7 002 0 2 Tey TNT CPGE 2796 1996 OVSE BESE OL UIAWON ON 31110305 3SIMAJHIO 551111 1941331001 DIAILNAIDS M WEEK 81270 31 29 10 9 31Y0 ANG Qddv 820 0V2 3770 2400 2131 LN3I2S 832551 OMUJHL NOYA 10155 34 N31LIUM sue no m EE EE REESEN 11883554 11111 OMG YON 3904004 ANY 104 SUJHIO OL 03501510 JA 01 LON SI NOILYWYOJNI HONS ONY NOIIVNUOJNI HOLWBNONI 31242 14318 531835 01 3AVN LYVd TAGON AYVLILYdONd SNIVINOD LN3AQ200 SIHI 305835 JUNLYYIANIL 0167791062 LIJ 00g 9112451 49270061 1104415 108 YOLOW 016 1 16 227006 OL 30181101 1 18 908006 11883558 4 1 1804 553334 206006 31114001 SSY19 026006 YJHSYM NOTAJI 110061 83835 SHL SS B E
11. Accessory Outlet 75 watts max e e O Ol gt O A Sav WAX A MAK A Remote SE Air 7 Cord Alarm Contacts onnector 1 Tank ntake 30V max 1A max RJ Il telephone style connectors IR CO units only Figure 1 19 Back of Cabinet Filling the Humidity Fill che humidity pan with sterile distilled water to within 1 2 of the top Pan Place the pan directly in the center of the incubator floor For applications requiring high humidity the pan should be placed against the left side wall of the incubator The side ducts have been modified to allow the pan to be placed against the wall Optimum humidity is achieved by capping the CO sample port This will however cause condensation in the chamber To enhance RH recovery from door openings place a second humidity pan in the right side duct For best operation of the incubator sterilized distilled demineralized or de ionized water should be used in the humidity pan Water purity should be in the resistance range of 50K to 1M Ohm cm or a conductivity range of 20 0 to 1 0 uS cm Refer to ASTM Standard D5391 93 or D4195 88 for measuring water purity Distillation systems as well as some types of reverse osmosis water purity systems can produce water in the quality range specified Tap water is not recommended as it may contain chlorine which c
12. Configuration mode NO NC ALARM CONTACTS i ALARIVI ALIS green wire red wire 30V max at 1A max COM not connected yellow wire black wire AAA Alarm Contacts Junction Box IMPORTANT USER INFORMATION Caution Stored product should be phone cable to incubator alarm contacts RJ 11 connector protected by an activated alarm system capable of initiating a timely response All outputs shown 24 hours day These alarms provide in alarm condition interconnect for centralized monitoring Figure 6 1 Remote Alarm Contacts CAUTION Contains Parts and Assemblies r Susceptible to Damage Electrostatic Discharge ESD Direct Heat C02 Incubator 6 1 Section 6 Factory Options 6 2 Humidity Readout Direct Heat C02 Incubator Series 8000DH incubators are equipped vvith a humidity sensor to monitor the relative humidity inside the chamber The sensor is located on the chamber ceiling and provides a signal that is displayed in 196 increments on the control panel The humidity readout can display continuously or toggle with the temperature readout In addition a low alarm limit can be set on the humidity readout to detect when the humidity pan runs dry Refer to Section 3 Configuration The sensor is capable of measuring relative humidity from 1096 to 10096 with an accuracy of 5 above 90 See Section 2 Calibration for details on calibrating the humidity readout Factors w
13. v Ajojeuurxoudde je JOOyo our OB m AJUBJJEM v os euin Buiddius SMOJJE siu Apre uno wos peddius si 3ueuudinboe moi ayep v WOJJ SUJUOW OM suejs POLY AJUBJEM ALNVYYVM 83 1V3G IVNOILVNAJLNI OIdILN3I9S AAHSIA Thermo Fisher Scientific 401 Millcreek Road Marietta hio 45750 United States www thermofisher com
14. 3 3 22 GREE 54 2474 BLACK 4 3 22 GRAY 55 24 4 WHITE 88 5 35 22 PURPLE 55 24 BLACK 89 6 36 22 RANGE 57 24 4 RED 7 1 ORANGE 37 22 31 BLACK 58 24 4 GREEN 90 8 BLACK 38 22 3 RED 59 o 9 YELLOW 39 22 3 WHITE 50 20 40 22 2 RED 92 41 22 2 BLACK 94 95 96 97 98 go 00 01 02 04 MODEL DESCRIPTION 3541 STERI CYCLE TC CO2 INC 230V 06 3543 STERI CYCLE IR CO2 INC 230V 07 IN 4502 10 15 14 DAG SAG CCS 360283 WAS 360230 7 10866 12714712 GLS GLS LON REVISED RH SENSOR CONNECTION 6 JIN 02 24 10 GJG GJG LON REVISED MOTOR PART NUMBER 5 SI 9982 04 25 09 KOG KOG LON 190793 WHT DISPLAY TO 190609 BLU 4 IN 3759 01 22 07 GJG GJG LUN ADDED DH 8000 SERIES MODELS 5 A 3 bad 08 02 03 AN KOG LON CHANCED 290137 see TU 2801 Electrical Schematic ee ra zene anni Model yuma er Rem E n n 230 Ster i Cycle ERU Sey Heh Heot Sterilization ATTENTION ER M ME KO m Gy rud avo OF leren SE NONE Incubotor OBSERVE PRECAUTIONS MATER AL ELECTROSTATIC ThermoFisher PAINT COLOR 371 70 0 0 REV 8 SENSITIVE DEVI CES SCIENTIFIC en 25500 EAS m Page j of 3 BOX 649 MARIETTA DHIO 45750 XXX 9 6 Direct Heat C02 Incubator Thermo Scientific EEST 60 7 V MAH V006 OSI uoneuuojur Up UW JOJ J01nqu1 SIP 18201 INOA 196 09 ven u pis n0 uor
15. Alarm Indicator Light pulses on off during an alarm condition MODE Select Switch Used to select Run Setpoints Calibration and Configuration modes Message Display Shows system status Mode Select Indicators RUN Run Menu SET Set Points Menu CAL Calibration Menu CON Configuration Menu HEAT Indicator Lights when heaters are activated INJ Indicator Lights during CO injection into chamber T C Display Programmable to display temperature See Configuration section RH 96 Display Shows percentage of humidity See Configuration sec tion CO 96 Display Programmable to display CO percentage See Configuration section Arrow Keys Scroll for Parameters Increases or decreases number val ues toggles between mode choices ENTER Stores the selected value into unit memory Silence Switch Power Switch Alarm oe Mode Selector Indicator Switch ENTER Temperature in button Mode Select ndicators Active Heat Indicator SEA A RUN SET CRL CON o o o o De Message Display SCROLL FOR PARAMETERS Scroll Arrow Keys Active Injection Indicator Humidity in Figure 1 2 Control Panel 1 2 Direct Heat C02 Incubator CO level in 96 Thermo Scientific Keypad Operation Thermo Scientific Section 1 Installation and Start Up The Series 8000DH incubator has four basic modes for incubator setup Run Setpoint
16. Low CO Alarm The low CO alarm limit is the deviation from the CO setpoint which imi H will cause a low CO alarm The setpoint is variable from 0 596 CO Limit tracking alarm below setpoint to 5 096 CO below setpoint The factory setting is 1 0 CO below setpoint A minus sign in the display indicates that the alarm setting is below the setpoint 1 Press the MODE key until the CON indicator lights 2 Press the right arrow until CO2 LO X X shows in the message display 3 Press up down arrow to change the low CO alarm limit 4 Press ENTER to save the low CO alarm limit 5 Press the MODE key to return to RUN mode or right left arrow to go to next previous parameter Set Hi h CO Alarm The high CC alarm limit is the deviation from the CO setpoint which S high I h le fi 3 Limit trackin alarm will cause a high CO alarm The setpoint is variable from 0 5 CO g above setpoint to 5 096 CO above setpoint The factory setting is 1 096 CO above setpoint 1 Press the MODE key until the CON indicator lights 2 Press the right arrow until CO2 HI X X shows in the message display 3 Press up down arrow to change the high CO alarm limit 4 Press ENTER to save the high CO alarm limit 5 Press the MODE key to return to RUN mode or right left arrow to go to next previous parameter 34 Direct Heat CO2 Incubator Thermo Scientific Section 3 Configuration Enable CO Alarms to High and Low CO alarms can be pr
17. Mods AUDIBLE XXX Enter Press Enter to AUDIBLE Ee Audible Alarm save the setting for Parameners Press to return Audible alarm OFF to previous parameter Press Enter to NEW HEPA Filter NEW HEPA restart the timer and clear x r the REPLACE HEPA alarm Press to return to previous parameter Numbers increase I ose REPLHEPA XX ESA Press Enter to save REPLACE HEPA Timer MM the chosen of months Press to return Numbers decrease to previous parameter Numbers increase pee m EX AOC OUDE save the setting el for Puy meters Press to return E R 1 215 HESS to previous parameter lt Numbers decrease Numbers increase Low Temp Alarm Limit TEMPLO XX Press Enter to save the setting Scroll fce Parameters Press to return Numbers decrease to previous parameter Temp relay ON Low Temp Alarm Relay TMP RLY XXX Press Enter to save the setting Semil inr Pme rs Press to return Temp relay OFF to previous parameter continue on next page 3 8 Direct Heat C02 Incubator Thermo Scientific Section 3 Configuration Configure Mode Chart 3 1 Page 2 of 3 refer to previous page To Configure Numbers increase Press Enter to save the setting Low CO Alarm Limit 0210 Scroll for Parameters Press to return to previous parameter Numbers decrease H cili Numbers increase Press Enter to save the setting High C
18. SET mode press the MODE key until the SET indicator lights Press the right and or left arrow keys until the proper parameter appears in the message display See Chart 1 1 for more detail Setting the Operating All Series 8000DH incubators have an operating temperature range of Temperature 10 C to 50 C depending on ambient temperature The incubator is shipped from the factory with a temperature setpoint of 10 C At this setting all heaters are turned off Thermo Scientific Direct Heat CO2 Incubator 1 13 Section 1 Installation and Start Up Setting the Operating To change the operating temperature setpoint Temperature continued Press the MODE key until the SET indicator lights 2 Press the right arrow until Temp XX X is displayed in the message display 3 Press the up down arrow key until the desired temperature setpoint is displayed 4 Press ENTER to save the setpoint 5 Press the MODE key until the RUN indicator lights for RUN mode or press the right left arrow keys to go to next previous parameter Setting the Overtemp Caution The independent overtemp system is designed as a safety to Setpoint protect the incubator only It is not intended to protect or limit the maximum temperature of the cell cultures or customer s equipment inside the incubator if an overtemp condition occurs A Series 8000DH incubators are equipped with a secondary temperature monitoring system to monitor the air temperature inside the cabi
19. X 2 018 900066 OLON 3300018 85 96 02 1 833149 0S2 2 990121 2 13081310 11001 INN Y3H SS 06 91 01062 N0114132 30 1894 ON 184 SIvig31VN JO 1118 LNN ONIM 15082 SYM 01062 ovs we eo vi ve evec vi 531435 0008 Ol NOHNO2 03004 oro oro ro ze io esie nif z NOILONGOYd UOJ 03511138 o eoo eoo roiv va o J4 JO 40114192930 leev ovo xs on noa asu G 7 002 O0L UJENAN DN Section 8 Spare Parts Thermo Scientific Direct Heat CO2 Incubator 8 4 Section 8 Spare Parts COMMON TO 3598 3540 3541 3542 3543 370 BACK PANEL ASSEMBLY 370 200 5 Rev 8 Page 1 of 2 Thermo Scientific Direct Heat C02 Incubator 8 5 Section 8 Section title BILL OF MATERIALS NEM PART NO PART DESCRIPTION OTY 2201 6 32 x 2 SS PHP SCREW 2 22049 6 32 X 3 8 PHP SCREW 6 3 2300 6 32 ZP LKWASH HEX NUT 4 4 23024 3 8 SS FLAT WASHER x 5 23058 6 SS EXT TOOTH LOCKWASHER 2 6 3401 12 SNAP IN HOLE PLUG BLACK 1 190222 VALVE SPACER 2 8 190994 ACCESSORY OUTLET MOUNT 370 380 ONLY 9 220259 ACCESS OULET LABEL 0 220381 ALARM CONTACT LABEL 220490 REFER TO MANUAL LABEL 2 220565 RS 485 LABEL 3 220566 CO2 1 LABEL 4 250139 SOLENOID VALVE 12V 5 460024 SNAP IN OU
20. cabinet temperature to stabilize Place the calibrated instrument in the center of the chamber The instrument should be in the airflow not against the shelf Temperature Stabilization Periods Startup Allow 12 hours for the temperature in the cabinet to stabilize before proceeding Already Operating Allow at least 2 hours after the display reaches setpoint for temperature to stabilize before proceeding Direct Heat C02 Incubator 2 1 Section 2 Calibration Temperature 1 Press the MODE key until CAL indicator lights continued 2 Press the right arrow until TEMPCAL XX X appears in the message display 3 Press up down arrow to match display to calibrated instrument 4 Press ENTER to store calibration 5 Press the MODE key to return to RUN or the right left arrow to go to next previous parameter Thermal Conductivity Models 3540 and 3541 have a thermal conductivity T C CO sensor CO System Thermal conductivity of the incubator atmosphere is not only effected by the quantity of CO present but also by the air temperature and water vapor present in the incubator atmosphere In monitoring the effects of COs air temperature and absolute humidity must be held constant so any change in thermal conductivity is caused by a change in CO concentration Changing temperature or changing from elevated humidity to room ambient humidity levels will necessitate a re calibration of the CO2 control Some T C CO sensors go
21. d NOLIdIdISJU 02 OVI AA 3110 UN N23 Aza YSEO0614 ASM TIAINJI 20 IN3W3OV 143 07313 aos SBSE 3 GESE DI NOWADO 1 8 002 86 838WIN ONIMVAC Thermo Scientific Direct Heat C02 Incubator 8 8 Section 8 Spare Parts H Fonn 8 8 006 01 FFXX 181330 SA TONY 3715 4JENNN ONIMYYO 03141934 3SIW83H10 SSTINN 32NVU3101 VIN INIVd OUWIU3IVN 0619 YO bi t ion xog quoudinb3 yuswuostaug p l1011002 N011Y404802 2373 00570 11y9 10 12 310 NOT QddV 820 QvO 3170 NA0 NOWHO 19 NQREGH NOOH INGOSSS NAREZKI MAULI I YUM INOHLIM 1904004 ONIYNLIVINNYA 10 0351 SYOSNAS 202 31111 OMG YON 35044 4 ANY 404 SYIHLO OL 035012510 18 OL LON SI NOILVAYOIN HONS NV NOL YRO04N1 YOLVENINT 531135 OLE 3WVN 18 4 7300 AYVLILYdOYd SNIVINOD 00 ATEWASSY 805815 1NV1V3 ALY 401 ONIY 13141701 111 16 62600611 01 OLE 4018 4 0760061 6 1901 3111040521 41172 4 NNOH 409435 Y 604184 L 80SN3S 4 02162 9 1395 9 0601 S 9NIHSQB dVNS 8 L 99006 13835 SAIL 4 1 X 8 06076 v LAN X3H HSVMYT dZ 9 0082 M382S dHd SS W E X 2 8 1022 ON ALO 0114 82530 1474 ON 1874 w i Sivid31VN JO 1119 531435 0008 OL NOWNOD 0300
22. return to the RUN mode or right left arrow to go to next previous parameter Thermo Scientific Set Lovv Temp Alarm Limit tracking alarm Enable Lovv Temp Alarm to Trip Contacts Thermo Scientific Section 3 Configuration The low temp alarm limit is the deviation from the temperature setpoint which causes a low temp alarm The low temp alarm is variable from 0 5 below setpoint to 5 below setpoint The factory setting is 1 below setpoint A minus sign in the display indicates that the alarm setting is below the setpoint 1 Press the MODE key until the CON indicator lights 2 Press the right arrow until TEMP LO X X shows in the message display 3 Press up down arrow to change the low temp alarm limit 4 Press ENTER to save the low temp alarm limit 5 Press the MODE key to return to RUN mode or right left arrow to go to next previous parameter The low temperature alarm can be programmed to trip the remote alarm contacts A setting of ON allows this an OFF setting blocks the low temp alarm from tripping the contacts The factory setting is ON 1 Press the MODE key until the CON indicator lights 2 Press the right arrow until TMP RLY XXX displays 3 Press the up down key to toggle the setting ON OFF 4 Press ENTER to save the setting 5 Press the MODE key to return to RUN mode or the right left arrow to go to next previous parameter Direct Heat C02 Incubator 3 3 Section 3 Configuration Set
23. the temperature cools down to the original set operating temperature or 30 C whichever is higher the display toggles between CYC CANCELED REPL HEPAs and PRESS ENTER The white LED is lighted but no longer flashes With IR CO sensor When the temperature cools down to the original set operating temperature or 30 C whichever is higher the display toggles between CYC CANCELED POWER OFF and REPLACE IR The white LED is lighted but no longer flashes After the unit is powered back on with the IR sensor reinstalled the display toggles between CYC CANCELED REPL HEPAs and PRESS ENTER Thermo Scientific Remote Alarms Thermo Scientific Section 6 Factory Options A description of the factory option connections to external equipment follows A set of relay contacts is provided to monitor alarms through an RJ11 telephone style connector on the back of the cabinet Refer to Figure 6 5 for the location of the alarm connector The 12 foot telephone cord P N 190388 and RJ11 to screw terminal conversion box P N 190392 are available through your local distributor The remote alarm provides a NO normally open output an NC normally closed output and COM common Refer to Figure 6 1 The contacts will trip on a power outage or an overtemperature condition The contacts may also be programmed to trip or not trip on temperature alarms CO alarms and RH alarms See Section 3
24. work A Two stacking brackets shown at left are included in the parts bag shipped with each incubator Figure 1 3 Stacking Brackets 1 Remove the cover plate securing the door cord from the incubator to be on top See Figure 1 4 Disconnect the plug from the connector 2 Remove the four screws securing the door hinges to the unit Remove the door and set it aside Cover plate and bolt hole with door connector HH LS Figure 1 4 Cover Plate Figure 1 5 Hole Plug Locations 3 Unscrew the two hole plugs from the top cover of the incubator to be the bottom of the stack Figure 1 5 4 Unscrew and remove the 4 leveling feet from the unit to be stacked on top and lift it onto the bottom unit Align all sides Warning This incubator weighs 260 Ibs 118kg Have sufficient personnel available when lifting Lift the unit by the sides of the cabinet base to avoid placing stress on the outer door hinge A Figure 1 6 Stacked Direct Heat C02 Incubator 1 5 Section 1 Installation and Start Up Stacking the Incubators cont Preliminary Cleaning 1 6 Direct Heat CO2 Incubator 5 Insert the stacking brackets into the slots at the back of the stacked units as shown in Figure 1 7 6 Align the slotted holes in the brackets with the mounting holes on the a i bracket bracket Nit Top incubator back n 00000000000000000000000 P back of the top incubator Secure
25. 00283 det maa Outer door switch 360236 m Sterilization initiation switch I 3500700000 Blower wheel 100085 a ee Access port filter assembly 760210 Disposable in line filters 10 filters 770001 Disposable filter 99 9796 CO2 line air sample 760179 qai Robes or e a e es HEPA filter 5 5 diameter 760209 Ep HEPA value pack 4 filters 1900067 HEPA filter replacement kit I90650 odes Four bada Independent inner door kit 190666 Ee EE EE Right hand door swing 19000691 eroe rir Roller base KROS S Overlay 50109579 AguaTec water cleaning cell 4 pack for water reservoirs pans bottles 1900346 Display control board 0060 ya Spring 1 for updated duct sheets 130217 oes Grommet 1 for updated duct sheets 1900567 Exterior door assembly SRO Thermo Scientific Direct Heat CO2 Incubator 8 1 Section 8 Spare Parts 2 002 01 EN e TVW1230 END u38NQN 9NIMVSO ASIMYIHLO SSIINN JINVUJTOL 311HM 1NIVd Tv T331YN 05157 OIHO 71131178 699 108 DIA LN312S Jeusi4ouueu L 0 awos 10 9 07 avo 340 dddy 850 072 310 NMO Al8W3sSv 31111 OMG 8 1 8 1 11242 1831 531835 OLE 3AVN 18Y47 13004 2131183125 130511 OMUJHL NOYA NOISSINU3d NILLIYM 1940 4 SNIVLNOD LNIWNODC U3HSYM 3N021118 01 690061 114 004 ONT Yds 492Y0061 1804415 SOLON OLEJI 16 227006 401 8
26. 0087 6 l HOLIMS 2 07831 1 Gezo E T O ZE I 332Y48 NOVEL 22062 E I TIVI30 GENA dW31 HJIH all 220021 2 1H9I3H SONTH 259 SONTH SS 601911 55770 4 ol 627 X 5 8192 X 5102 390601 0 2 1396 70 39NIH SOOO 511801 l ONIHSRB dWNS 2 1 1 Lt 8 11 ONIM S S 22 86 16082 L AJHSYM 07 TVNAJINI 9 2 9 SS 86 12022 B z NSAJS dHd SS bai X 22 90 51122 y 5 MUROS dHd SS B E X 22 8 epes 3 ol NJAJS ad SS W I X 22 82 6022 2 y M33JS IM dHd SS X Oz v 18 86002 l AI 1 18BVd ON lavd are NISSIVW 40 7118 8 11 Direct Heat CO2 Incubator ientific Thermo Sc Section 9 Electrical Schematics O O O I S on Ur W N MB NM INO 2 E O IN UJ POWER CONNECTION 115VAC 50 60HZ IPH 9 6 FLA b 2 bd 3 6 e a 1A FUSE 1 54 230135 15 230 D A Y 4 s sj B JE 8 J e e m m m 5 2 5 4 10 Sf 5 6 J3 1 O T LN OZ 8 3 no POWER SUPPLY m A m 3 4 I Le A 400132 FOR c KIT 190778 om iB zz DO 3 5 d Bas 12 L WEG mun rem B 6 gt 4 DISPLAY BRD ASSY 190508 RIBBON CABL
27. 018 31 3 1 1 80 0061 40 1994 OLE NOLIVINSNI 334018 Cid 006071 SNIHSQG 14YHS 140018 160061 INNOW YOLOW 4060061 YJHSYM NOT331 110061 MAYIS SHL SS B E X 0lk 900066 H3NHOJSNYHL VAM voloey IYISOWHJHI 091007 130018 56 1991 NOIIdIN2S30 1874 ON 18Vd STIVIUJIYN dO 1118 MILA 414181 18 310N 03007 JO NOlI1dIN2S3Q dree 196 opge 96S OL NOWNOD 8 e 002 01 OMO Thermo Scientific Direct Heat C02 Incubator 8 2 Section 8 Spare Parts m 2 01 002 01 FXX TWWI230 EEN 438NQN 9NIMVUC 03112345 JSIMUJHL 00670 31Y2 10 9 7 31Vd 40 0444 820 A18 YOLVENIN 31942 18318 S3183S OLE O SSTINN 2900011001 Y2 3190 NMQ W3JSSY 31111 940 JWYN 18Y4 1300W 05155 OIHO VI13IUVW 679 108 DIAITLNAIDS Jeusijouueu DWN MOU 00991 WOU 80 168 BAH IH 301 RUM IMOHLIM 3904804 ONIYNLIVINNVW 403 0351 YON 1904904 ANY 101 583110 OL 03501250 38 OL LON SI NOLIVWUOJNI HONS NV NOILVWUOJNI Auvi3ludOJud SNIVINOD IN3W0200 SIHI aG 3801 3N02111 040 uiv QUIT 9 3801 3N02111 02077 HIV 3114 g0 N3 3unlvu3dN3 1 81062 TANNVHD 113H S 1 16 799061 LIM 008 ONIUd S 19 h0061 180441 XOH HOION OL 16 227006 OL 40143 3 1 80 0061 41843957 815 LONG 401 531835
28. 38 pue on l k p uenem JOJ pa 2E U0J aq 1 juauniede 399 2014499 BU 1 186 AjueJeM siy Aq palaAo9 JOU ae 63 pue uone jeisu pepnjul 10 6 asuadxe s ouuau 1e p oeyd l Jo paJredai aq Wa diySUBUMJOM JO u Buruojuo uou aq 0 sued zuauodwos Ie s114 ay Buung ponad Ajuellem Jead 1911 Buunp saumo juanbasgns Aue 0 spu yx 0119910 0 AJURJJEM au Palanjap si 1u uidinb ino au aues y 1e 1294 8 OJU OB jim AUB UBA ay os Buiddius 104 SMOJJE siu Ayjmeg ino wos peddius si juawdinba ino aep ay WO1 SY M OM 51615 polad AueueM au ALNVAAVYM LONGOdd 0 0 15 21411N4195 d3HSIH OIWd3H1 034315103 60 Z v M V006 051 uoneoijdde 6120945 pue eoueuojureuJ uoge 1edo AueJje jueuudinbe uo suonsenb noA Jamsue O peaiJ al aM UOIJEWJOJU AJUBJJEM JOJ JOJNGIJJSIPJELO Jno 1963002 eoueuojuleuJ ABu A 1d pue uonejedo uonejejsur u uudinb jnjejeo sjenueuJ uononujsur Doud Saane jueuudinbe moi uoneuuojur uoneJedaud aus eAisueueJduioo YUM O peai si sejeg INOA SjonpouJd jo ssoj 10 sjijoJd so o BunejeJ soBewep Jnoyjim Buipniou soBewep jenuenbesuoo Jo yoosipul Aue JOJ e qei eq jou IlEUS AlddV TIVHS JSOddnd AVINDILYVd V SS3NLIJ NO ALFTI
29. 5 OUTPUT KIT 1900085 OPTIONAL RH SENSOR KIT 1900081 3588 3588 3584 3585 OPTIONAL ANALOG OUTPUT KITS 190512 4 to 20nA 190543 to 5 190544 0 to 1 0 DPTIUNAL GAS GUARD KIT 1900086 C02 PRESSURE SWITCH 1 TANK EMPTY 360213 PRESSURE SWITCH 2 TANK EMPTY 380213 SOLENOID 3 WAY l2VDC 250121 MODEL 381 IR 02 SYSTEM 231204 Model 230V Steri Cycle Incubator Electrical Schenatic Heat Sterilization Page 2 of 3 371 70 0 D REV 8 Thermo Scientific Direct Heat C02 Incubator 9 5 Section 9 Electrical Schematics 77 78 WIRE REFERENCE CHART No GA COLOR GA COLOR M GA cone 79 1 6 BROWN 21 18 RED 42 22 3 BLACK 2 16 BLLE 22 18 GREE 43 22 3 RED 80 3 16 URN YEL 23 18 ORANGE 44 22 3 WHT IR GRN 81 4 6 BLACK 24 18 GREE 45 22 BLACK S 5 6 WHITE 25 22 BLUE 46 20 RED 82 5 18 BLACK 26 22 BLUE 47 20 RED 3 7 16 YELLOW 27 22 BROW 48 20 BLUE 8 18 ORANGE 28 22 BROW 48 20 BLUE 84 9 18 RED 28 22 YELLOW 50 20 ORANGE 65 0 18 B LE 30 22 YELLOW 51 20 ORANGE 1 18 RED 31 22 RED 52 20 BLACK 06 53 18 BROW 87
30. 5 minutes or less is 30 000 ppm 396 CO Carbon dioxide monitors are recommended for confined areas where concentrations of carbon dioxide gas can accumulate A Warning This incubator is designed to be operated with CO2 gas only Connecting a flammable or toxic gas can result in a hazardous condition Gases other than CO should not be connected to this equipment CO gas cylinders have a UN1013 label on the cylinder and are equipped with a CGA 320 outlet valve Check the gas cylinder for the proper identification labels The CO gas supply being connected to the incubator should be industrial grade 99 5 pure Do not use CO gas cylinders equipped with siphon tubes A siphon tube is used to extract liquid CO2 from the cylinder which can damage the pressure regulator Consult with your gas supplier to ensure that the CO cylinder does not contain a siphon tube Gas cylinders should also be secured to a wall or other stationary object to prevent them from tipping A two stage CO pressure regulator is required to be installed on the outlet valve of the gas cylinder Input pressure to the incubator must be maintained at 15 psig 103 4 kPa for proper performance of the CO control system A single stage CO pressure regulator will not maintain 15 psig 103 4 kPa to the incubator as the pressure in the CO cylinder decreases therefore a two stage regulator is recommended Thermo Scientific Section 1 nstallation and Start Up Connec
31. CO2 setpoint may be entered immediately Otherwise it is important to allow the unit 12 hours to stabilize at the temperature setpoint before entering the CO setpoint Both models of the incubator have a CO setpoint range of 0 096 to 20 096 The incubator is shipped from the factory with a CO setpoint of 0 0 At this setting all CO control and alarms are turned off To change the CO setpoint 1 Press the MODE key until the SET indicator lights 2 Press the right arrow until CO2 XX X is displayed in the message display 3 Press the up down arrows until the desired CO setpoint is displayed 4 Press ENTER to save the setpoint 5 Press the MODE key until the RUN indicator lights to go to RUN mode or press the right left arrow keys to go to next previous parameter Direct Heat C02 Incubator 1 15 Section 1 nstallation and Start Up Chart 1 1 Set Mode Press MODE to light SET To Set perating Temperature ver Temperature Percent C0 m m ms s ei lm amp je m al Cer Press M DE to move to CALIBRATE E TEMP XXX C Press lt to return to previous parameter OTEMP XXX C Press lt to return to previous parameter C02 XXX Press lt to return to previous parameter SCROLL FOR PARAMETERS Numbers increase Press Enter to save setting Numbers decrease Numbers increase Press Ente
32. Cycle The Sterilization Cycle requires approximately 12 hours heat up 2 4 hours sterilize 2 hours and cool down 6 8 hours Additional time is needed to verify the calibration of temperature and CO after the cycle is complete During the Sterilization Cycle the incubator updates the temperature to the analog output board and the 1535 however CO will be fixed at setpoint and RH will be fixed at RH Low Limit plus 196 A HETA filter replacement kit see the spare parts list should be on hand prior to initiating the Sterilization Cycle Information About the Cycle Pre cleaning may be required In order to avoid odors stains on the interior baked on material etc wipe off all visible signs of spills Odor may occur during the Sterilization Cycle and is considered normal The Steri Cycle is not intended to sterilize other items instruments etc from the lab During the cycle the unit chamber becomes hot enough to melt samples instruments dishes etc left inside the unit The items listed below also need to be removed 1 HEPA filter 2 Air sample filter 3 Access port filter assembly 4 Water in the humidity pan 5 Temp RH recorder probe if applicable lt gt 6 IR sensor if applicable HEPA Access Filter N Air Sample During the Sterilization Cycle discoloration 5 Filter F ilter of some material
33. E 25 196018 31 S 44 2 E 27 gl J4 3 e 4 4 E J4 5 Zt 4 6 305 4 7 3 J4 8 e J4 9 ER 4 CDM 24 10 2 OPEN 24 11 35 3 CLOSED 36 1 45VDC POWER INLET RFI LINE FILTER 460180 POWER SWITCH CIRCUIT BREAKER 12A 230180 EXTERIOR CONVIENCE OUTLET 460024 TRANSFORMER IKVA 420104 INTERIOR FAN MOTOR LISVAC 50 60Hz 156153 COMPONENT COOLING FAN 900135 CONTROL T STAT COR 400151 DVERTEMP SAFETY MANUAL RESET Open on Rise 400150 CHAMBER HEATERS BACK 39 156W2 132058 BOTTOM 28 112W2 132058 LEFTC 23 92W 5 1 32060 RIGHT 23 92W 132061 COLLAR HEATER 48 192 132062 DOOR HEATER 89 356W 132057 STERILIZATION PROCESS INDICATION CD2 SOLENOID 12VDC 250138 DOOR SWITCH INNER 360239 Shown w door open STERILIZATIUN INITIATE BLITTUN 360236 DOOR SWITCH OUTER Shown w door open 360283 TEMP PROBE 2252 OHM e25C 290184 MODEL 370 02 SENSOR 290168 Electrica Schematic Model L15V Ster i Cycle Heat Sterilizotion Incubator 370 70 0 D REV 10 Page of 3 Thermo Scientific Direct Heat CO2 Incubator 9 1 Section 9 Electrical Schematics 5 4 ak 0 N ell 4 REMOTE ALARM CONNECTIONS 41 m 43 RJ 11 shown in alarm state bul g PES 42 a 43 as
34. GJG LON REVISED MOTOR PART NUMBER 6 S1 9982 04 25 09 K t KDG LON CHG 190793 WHT DISPLAY TO 190609 BLU 5 IN 3758 01 15 07 GJG GJG LON ADDED MODEL NUMBERS 4 IN 3308 05 15 06 GJG GJG MSB ADDED MODEL NUMBERS 3 IN 3124 08 04 03 JN KDG LON CHANGED 290137 TEMP PROBE TO 290184 2 IN 2870 08 04 01 GLM K t LDN CHG HARNESS FOR MICRO TO SENSE VOLTAGE LOSS 1 IN 2815 04 03 01 GLM KOG DNE CHANGED 130625 COOLING FAN TO 900135 0 N A 11 23 01 Q Gy NF RELEASED PRODUCTION REV ECN NO DATE BY CAD DESCRIPTION OF REVISION ATTENTION OBSERVE PRECAUTIONS ELECTROSTATIC SENSITIVE DEVICES THIS DOCUMENT CONTAINS PROPRIETARY INFORMATION ANO SUCH INFORMATION IS NOT T BE DISCLUSEO TO OTHERS FOR ANY PURPOSE NUR MODEL PART NAME HEAT STERILIZATION INCUBATOR See Above OWG TITLE ELECTRICAL SCHEMATIC Electrical Schenatic Model 113V Steri Cycle Heat Sterilization Incubator USED FOR MANUFACTURING PURPOSES WITHOUT RITEN PERMISSION FRON TERMO FISHER SCIENTIFIC OWN Gy ran Qy Leen DNE DATE 9 12 00 SCALE NONE MATERTAL e 4 ThermoFisher PAINT COLOR 370 70 0 D REV 10 SCIENTIFIC TOLERANCE UNLESS OTHERWISE SPECIFIEO DRAWING NUMBER SIZE p f ANGLES DECIMAL si 370 70 0 D age 3 of 3 BOX 648 MARIETTA OHIO 45750 k XXXza Thermo Scientific Direct Heat C02 Incubator 9 3 Section 9 Electrical Schematics O
35. ITYY 8d JAAJSEO NOLINJLIY Van 3715 ONIMVaBO U3IJI33GS 3SIMNSHID SSTNI 32444310 DI4ILNAIDS NN 0 INIVd AJeusijoublet L VN V S31YA Seg 3WOS 90 08 90 31vG 854 Odav ors tava oro we 2131443105 AJHSIJ OMYSHL Hi NOISSTANJA NALITAN INOHLIM S3SOd Nd SNIAILIVATNVA 035 ATAWASSY 13738 TANYd 31111 ONG 40 ISOd Nd ANY X04 S 3HID OJSITISIO 38 UL LON SI NOTIVKOCANI HINS ONY NOILVKOCANI 531435 8662 Nu 18Vd 7300 13144044 SNIVINOIJ INJNIJOO SIHL 02 61 81 91 SI SSTIJA IMId TITA vGc061 y AJANOXI JI 721 X CO 7 1 T HOLT El SS OO 829 X JI 917 0900 el 1 lay y SSvaB law B I X JSDH 91 CE0078 1 9 Mis SWIS dHd v I X ZE 9 1 20066 DI dJldVOV SSvad 143 821 X ISOH KI 590078 6 B3HSVM IVIJ SS 8 E ycOEC 8 1 132788 130d S5E0061 L 1 HJLIMS S000 az 21134 06209 9 1 yel 1 5 s33y488 11181 081082 S 1 4 LIMIAIJ DD 7 006 F ALIH D HILIMS 37 18315 692096 Lg TANYd 1120061 gt x Z Lg AY Ou TANYd A8EOZ 1 ALO 114182930 1 yd 838BNIIN 1878 WALT SvIS3IVA 40 T1118 JLON 0300 5221 505 979 50 40 y0 ZIBE NI JLUN NUWADO OJSTAJA ASW 9 9 T B0 70 0 ZIBE NIJ 2 ALON Dl 0300Y SW 99 9092 40 51 10 SSZE NI 1 NOISIAZS
36. Mode Press MODE to light CAL 477 255 JI l Silence Mode CALIBRATE al Haus DER Scroll for Parameters LA 4 x 9 lt To Calibrate a Numbers increase 0 i Press MODE to move T 1 perating to SYS CONFIG m Temperature mode dutem cen save the setting Press to return L Numbers decrease to previous paramete I T Numbers increase Press Enter to 1 60 ode CO2CAL XXX Emer save the setting when Thermal Conductivity sensor is in use Press 0 return to previous paramete Scroll for Param eters 4 Numbers decrease ge t Numbers Increase IR CO Press Enter to 2 Mode IR CAL XXX m when IR sensor is in use Sr 1 save the setting Press to return Y Numbers decrease to previous paramete f Numbers increase Percent RH us ou OX m 7 when RH option is in use save the setting Presse to retur lt 4 Numbers decrease to previous parameter 2 6 Direct Heat C02 Incubator Clart24cdr Thermo Scientific Turn Audible Alarm ON OFF New HEPA Filter Thermo Scientific Section 3 Configuration Several features a
37. O Alarm Limit Mode CO2HI XX Scroll for Parameters Numbers decrease il H Press to return to previous parameter CO2 alarm relay ON Press Enter to CO2 RLY XXX save the setting Scroll for Parameters CO Alarm Relay Mus Press to return to previous parameter il H e 02 alarm relay OFF Numbers increase Press Enter to T C Z XXXX save the setting Scroll forParameters T C CO Zero Number Mode Press to return Numbers decrease Numbers increase Press Enter to T C Co Span Number Mode T C SE save the setting Scroll for Parameters 4112 Press to return to previous parameter Numbers decrease H H Numbers increase RH Low Alarm Limit Mode Press Enter to save the setting Press lt to return to previous parameter Scroll for Parameters hh o UE CIE Numbers decrease RH alarm relay ON Press Enter to save the setting RH alarm relay OFF Mode RH Alarm Relay 105 Scroll for Parameters Press to return to previous parameter H ei El refer to next page Thermo Scientific Direct Heat C02 Incubator 3 0 Section 3 Configuration Configure Mode Chart 3 1 Page 3 of 3 refer to previous page To Configure Display temperature ON Press Enter t DISP TMP XXX He Display Temperature DT 1 8 Press to return Display temperature OFF to previous parameter Display humidity ON Press Enter to save the
38. O C O CO 81 U uJ UJ El NON NON EN bi P CO O A N O O MO CD S O POWER CONNECTION 230VAC 50 60HZ IPH 4 4 FLA Ke 93 e BOH 0 54 FUSE ER 5 230120 230 0 115 l t RA 5 B 5 3 8 ru 4 10 aaa 5 2 sed 10 4 4 5 6 E J3 1 bi aes a LN POWER SUPPLY 2 400119 m E 18 s 400132 F R Ve KIT 190778 O Lu Cer Bi es biy Ep Ho 207 III gt pos Ze J9 2 PE 9 3 2 D A DISPLAY BRD ASSY 100509 34 RIBBUN CABLE 4 1 25 S 198018 l 25 4 2 oe J4 3 ela sk J4 4 2 2 J4 5 z 2 p 30 4 7 31 lt 2 J4 8 32 4 9 4 COM 24 10 x 2 OPEN 3 3 CLOSED v z 35 CL 45VDC POWER INLET RFI LINE FILTER 460180 POWER SWITCH CIRCUIT BREAKER 124 230180 EXTERIOR CONVIENCE OUTLET 460138 IEC 320 TRANSFORMER KVA 420104 INTERIOR FAN MOTOR ISVAC 50 60 2 156153 COMPONENT COOLING FAN 900135 CONTROL T STAT COR 400151 OVERTEMP SAFETY MANUAL RESET Open on Rise 400150 CHAMBER HEATERS BACK 39 158W 132058 BOTTOM 28 112W 132058 LEFT 23 82W5 132060 RIGHT 23 92W 132061 C
39. OLLAR HEATER 48 192W 132062 DOOR HEATER 89 356W gt 132057 STERILIZATION PROCESS INDICATION CO2 SOLENOID 12vDC 250085 DOOR SWITCH INNER 360239 Shown w door open STERILIZATION INITIATE BUTTON 360236 DOOR SWITCH OUTER Shown w door open 360283 TEMP PROBE 2252 OHMS e25C 290184 MODEL 3717 02 SENSOR 290168 Electrical Schenotic Model 230V Ster i Cycle Heat Sterilization Incubator 3 1 70 0 D REV 8 Page of 3 9 4 Direct Heat CO2 Incubator Thermo Scientific Section 9 Electrical Schematics Jr CD O OF OT UI OI Ol i Or CH P ei er ET Ay 0 JO CO ET E EI dus WS 65 4 9 76 x V J 4 s N i 1 38 uz RH 39 2 1 46 5 43 465 485 eil 42 RS 485 m y 43 HIE 2142 l JI 2 m m 2 r h J1 3 LI gt DEI hl e es EL Ji 6 ay con 1 7 TEMP SC ANALOG CO2 id DUTPLIT BOARD S i RH n O 02 J13 1 3 2 Ate J13 3 49 Ce N C J13 4 48 50 13 5 e J13 6 26 J13 7 e J13 8 J7 1 8 K 2 56 7 3 7 4 se 27 5 viel 58 J7 6 SS BOG m REMOTE ALARM CONNECTIONS shown in alarm state OPTIONAL RS 48
40. RH 44 OPTIONAL RS 485 OUTPUT KIT 0 1900085 45 U ell 42 RS 485 46 143 A JI I S 7 31 2 s m i y T 5 OPTIONAL RH SENSOR KIT 48 r h JI 3 1900081 1 4 vl bi 3568 J 45 42 2 49 JI 5 3598 7 m con Jl e S 50 ol TEMP 32 OPTIONAL ANALOG OUTPUT KITS 5 ANALOG 02 g 190512 4 to 20mA 23 210 Hu 190943 0 to SVOL BOARD te 180544 0 to IVDC 54 Ms S c 02 35 8 56 46 OPTIONAL GAS GUARD KIT 5 J13 1 e 1900086 C02 N C S 413 2 7 Mapa PRESSURE SWITCH 1 TANK EMPTY 360213 58 ER 48 PRESSURE SWITCH 2 TANK EMPTY 360213 ES 59 413 ER 60 J13 5 RES i 51 M ER SOLENJID 3 WAY 12VOC 61 pon M Du 250121 MODEL 380 54 IR CO2 SYSTEM D Jide E 231204 K 5 54 7 35 65 J7 4 23 7 5 66 7 8 9 67 2 S 868s D lt o Xo D a 6 CJ 70 8 71 72 73 74 Electrical Schenotic Model MIV Steri Cycle 76 Heot Sterilizotion Incubator 370 70 0 0 REV 10 Page 2 of 3 9 2 Direct Heat C02 Incubator Thermo Scientific Section 9 Electrical Schematics 77 78 79 80 82 83 84 85 86 87 88 88 LO O to J LO E RN tJi 2 c WIRE REFERENCE CHART NO GA cum
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42. Section 5 Routine Maintenance 5 12 Sterilization Cycle continued Direct Heat C02 Incubator 10 Open the chamber and install a nevv HEPA filter air sample filter and access port filter assembly Press ENTER 11 When ENTER is pressed the LED goes out the display returns to SYSTEM OK and the HEPA filter replacement timer is reset 12 Fill the humidity pan with 3 liters of sterile distilled water with the recommended purity range See Section 1 13 Allow to stabilize for at least 12 hours at the required temperature and CO level It is recommended that the temperature and CO calibrations be verified periodically during the first week of returning the unit to service Caution The high temperature sterilization cycle may cause the T C CO sensor output to change significantly This is normal and does not indicate damage to the sensor Therefore it is essential that the CO2 calibration is verified before returning the unit to service A If an independent instrument is not available to verify calibration the following procedure may be performed After the Sterilization Cycle and before CO is allowed to enter the cabinet 1 Fill and install the humidity pan 2 Allow to stabilize at the desired operating temperature for at least 12 hours 3 Calibrate the display to 0 096 Note If the Cool Phase is canceled the display toggles between CYC CANCELED and COOL PHASE With T C CO sensor When
43. TLET WHITE 370 380 ONLY 6 460138 POWER OUTLET 311 381 ONLY 1 460180 POWER ENTRY RFI LINE FILTER 8 600034 SNAPPER CLAMP 375 3 9 600063 SNAPPER CLAMP 312 20 130044 SHOULDER WASHER 375 ID 21 110001 DISPOSIBLE HEPIA FILTER 174 22 840008 5 32 TUBING ADAPTER 2 23 900135 3 15 SQUARE FA 24 1900313 BACK PANEL 25 310 C02 INLET 2 12015 3 16 VINYL TUBING 6 26 310 C02 INLET I 950013 VINYL INLET TUBE 370 8 X 01 840020 FTO 250 BULKHEAD FITTING 28 840020 NUT 315 NUT BLKHD FITTING 29 840020 WSHR 375 IT WASHER BLKHD FIG ITEM 15 PART OF KIT 2270112 NOT SOLD SEPARATELY 370 200 5 Rev 8 370 BACK PANEL ASSEMBLY Page 2 of 2 8 6 Direct Heat C02 Incubator Thermo Scientific Section 8 Sect ion title ni YO lb l h 6y9 xog quoudinb3 p ll0 002 N011Y404802 2373 ouey L E d 9 004 016 26 TWNI230 SJTOW 3115 UJEKON ONIAYUO 31112305 3S1MU3HLO SS31NQ 32NY83101 VIN INIVd 00970 31725 1078276 31VQ 101 Odd 820 042 3170 NMO N0319311 OWYFHL WOH 7 NOISSINH3d NJILIUM ASSW AV81 TINVd TOMLNOD 31111 940 IMOHLIM JSOddNd ONTYNLIVANNYW 101 03 YON JSOdANd ANY 403 SU3HLO 01 GISOTISIC 38 WolvenoNI 531135 OLE 3WVN 10Y471100N OL LON SI NOIIVNUOJNI HONS NV NOILYWYOJNI AUWLALYdOYd SNIVINOD LNAWNDOD SIHL
44. Y 9 9 9r9 L0 22 10 6SLE NI v 1 310N 0300Y 851 9 9 9 9 90 08 90 9088 31Y14 HIR 2610061 0300Y YOSNIS DL 01 90 TOf 10 12 60 L962 NI 2 WOSN3S 31 OL SINN Y ONIH 9110061 Q3QQV NO1 90 19 10 90 10 6162 NOILONGOYd 804 03511130 N01 820 820 I0 v2 rO V N 0 NOISIA3N 40 NO 1dIU2S30 ddv 0721 48 310 ON N23 38 680818 ISN A 181557 YOSNAS 21 1NY1Y3 60611 ALY ISN 401 UJANVHO INVIVIS ALY 640818 ISN ONIY 19314401 11 16 92600611 6 ONIY dOLS 21 1260061 8 3114 131138 OL 01 2610061 z ONIY 9N N Y31 33 SM01138 8110061 9 INNON DI 3004119 1041 2800061 S 905135 21 dN31 IE 891062 v ani 1311101021 zm LAN X3H HSY M T dZ 26 94 10062 2 1312 44 092 26 91 12122 I 0114182530 LUV ou nva N S VIg31V 40 1118 1y 766 1766 0796 BESE OLNOWNOO 8 8 002 01 UJENNN OMG 8 9 Direct Heat CO2 Incubator lentitic Thermo Sc Section 8 Spare Parts 2 40 abog C Ma 0 0 5 00c 865E EVSE 9 CET VSE OFSE OL MM 1 A TEWASSY x Thermo Scientific Direct Heat C02 Incubator 8 10 Section 8 Spare Parts Z 10 z abog C AS 0 6 002 B6S Dn TIV1S0 HOLIMS X000 JINNI 05 0 BDN3 4000 JINNI
45. a readout only of the chamber relative humidity level It does not provide any control of the relative humidity in the cabinet Relative Humidity Stabilization Times Start Up Allow 12 hours for the relative humidity and temperature in the chamber to stabilize before proceeding Already Operating Allow at least 2 hours after temperature display reaches setpoint for relative humidity to stabilize before proceeding 1 Place an accurate independent instrument in the center of the chamber Allow at least 30 minutes for RH to stabilize 2 Press the MODE key until the CAL indicator lights 3 Press the right arrow key until RH CAL XX appears in the message display 4 Press the up down arrow to match the display to the independent instrument 5 Press ENTER to store the calibration 6 Press the MODE key to return to RUN mode Thermo Scientific Section 2 Calibration Relative Humidity If a reliable RH measuring device is not available the display can be co ntinued calibrated to a typical level 1 Follow the RH stabilization periods outlined above 2 With a full humidity pan and stable temperature the relative humidity in the chamber will be 95 3 Using Step 3 5 of the relative humidity sensor adjustment adjust the display to 9596 This calibration method should be accurate to within 5 Thermo Scientific Direct Heat C02 Incubator 2 5 Section 2 Calibration Chart 2 1 Calibrate
46. abinet heats to sterilization temperature RUN SET CAL CON Se z STERILIZING s A v RUN SET CAL CON NI Z HEAT PHASE 8 Z Figure 5 15 Heat Phase 7 When the air temperature in the unit reaches 140 0 C the Sterilization Phase begins and the display changes to STERILIZING ANO an o en 440 ren n RH Z STERILIZING INJ CO Z de D Figure 5 16 Sterilization Phase 8 After approximately 2 hours a 5 second audible tone sounds signaling that sterilization is complete The Cool Phase begins The display toggles between STERILIZING and COOL PHASE AUN SET CAL CON o STERILIZING A v RUN SET CAL CON o o o o COOL PHASE Figure 5 17 Cool Phase 5 10 Direct Heat C02 Incubator Thermo Scientific Sterilization Cycle Thermo Scientific continued Section 5 Routine Maintenance 9 Completed Sterilization Cycle with T C CO sensor When the temperature cools down to the original set operating temperature or 30 C whichever is higher the display toggles between CYC COMPLETE REPL HEPAs and PRESS ENTER The white LED is lighted but no longer flashes Completed Sterilization Cycle with IR CO sensor When the temperature cools down to the original set operating temperature or 30 C whichever is higher the display toggles betw
47. an deteriorate the stainless steel Tap water may also have a high mineral content which would produce a build up of scale in the pan High purity or ultra pure water is not recommended as it is an extremely aggressive solvent and will deteriorate the stainless steel Thermo Scientific Direct Heat C02 Incubator 1 11 Section 1 nstallation and Start Up 1 12 Filling the Humidity Pan continued Connecting the CO Gas Supply Direct Heat C02 Incubator High purity water has a resistance of above 1M to 18M Ohm Even high purity water can contain bacteria and organic contaminants Water should always be sterilized or treated with a decontaminant safe for use with stainless steel as well as safe for the product prior to being introduced into the humidity pan Check the level and change the water frequently to avoid contamination Do not allow the water level to fluctuate significantly Dry outs will have an adverse effect on the humidity level and CO calibration of the T C units Caution Use of chlorinated water or decontamination products containing chlorine will deteriorate the stainless steel and cause rust voiding the warranty A Warning High concentrations of CO gas can cause asphyxiation OSHA Standards specify that employee exposure to carbon dioxide in any eight hour shift of a 40 hour work week shall not exceed the eight hour time weighted average of 5000 PPM 0 596 CO The short term exposure limit for 1
48. claving the glass doors should be avoided There is no simple method for repairing corroded glass In most cases the glass must be replaced Clean Humidity Pan the humidity pan with soap and water and a general use laboratory disinfectant Rinse with sterile water and spray with 70 alcohol The pan may be autoclaved HEPA Filter Replace the HEPA filter when the REPLACE HEPA reminder is Maintenance displayed The REPLACE HEPA reminder can be set to alarm after a specified time from 1 to 12 months The reminder default is the factory recommended setting of 6 months For details see Section 3 Replace Power Fuses To access the only replaceable fuse in the incubator Thermo Scientific 1 Turn off the incubator s power switch and unplug the power cord 2 Remove the two screws from the top of the control panel Grasp the control panel on each side and pull straight outward 4 Figure 5 3 shows the location of the fuse See Table 5 1 following for replacement fuse specifications 5 Secure the control panel and return the unit to service If the fuse blows after restoring power to the incubator contact your local distributor Table 5 1 Fuse Replacement Chart Fuse Voltage Manufacturers P N Amperage Rating Rupture Speed IEC Letter Code 230VAC BUSS GMC 0 5A 500mA Time Lag T 115VAC BUSS GMC 1 0A 1 0 Amp Time Lag T Direct Heat CO2 Incubator 5 3 Section 5 Routine Maintenance Replace Air Sample 1 Connect one end of t
49. e first power the unit off Then turn the two 1 4 turn fasteners on the top duct until the cover plate is disengaged Secured on the inside of the plate is a dummy sensor Figure 5 6 A on ii t Figure 5 6 Dummy Sensor Figure 5 7 IR Sensor with Cable Grip the IR sensor and carefully pull it downward Unscrew the cable from IR sensor Figure 5 13 Set the sensor aside See Step 9 for the IR sensor disinfection procedure Connect the dummy sensor to the cable Fit the installed dummy sensor up into the previous sensor location When the power is turned on with the IR sensor removed the display toggles between REMOVE HEPAs REMOVE WATER and PRESS ENTER If ENTER is not pressed within 1 minute the display toggles between POWER OFF and REPLACE IR When the unit is powered back on with the IR sensor reinstalled the display returns to SYSTEM OK RUN SET CRL CON o o o o REMOVEHEPAs A v RUN SET CAL CON o 5 o o PRESS ENTER A v RUN SET CRL CON o 5 o o REMOVEWATER Figure 5 14 Displays with IR Sensor Removed Direct Heat C02 Incubator 5 9 Section 5 Routine Maintenance Sterilization Cycle 6 After ENTER is pressed the Heat Phase initiates The white LED i flashes and the display toggles between STERILIZING and HEAT continued PHASE During this period the HEAT light will be on and the c
50. e of the channels installed on the right side duct Thermo Scientific Direct Heat C02 Incubator 1 7 Section 1 nstallation and Start Up Installing Duct Sheets Locate the supplied rod spring and end pieces 4 each Assemble the and Shelves cont spring to the rod by positioning the very end of the spring over the ridge at the rod Then press the spring to the opposite side It should snap into place See Figures 1 13 and 1 14 7 go sd Figure 1 13 Spring End Figure 1 14 Spring Assemble to Rod 7 Slide end cap over spring Figure 1 15 Note When installing the rods the spring end can be installed on either side of the chamber The upper front rod is high in the chamber and the upper back rod is Figure 1 15 Spring End lower to allow access to the access port filter door A 8 Install one end of the rod into the appropriate hole in the duct sheet 9 Compress the spring under the end cap to insert the other end of the rod into the hole in the duct sheet on the opposite side 10 Figure 1 16 shows the four Front opening of chamber E rods installed m Back wall of chamber Ne Figure 1 16 Four Rods Installed in Chamber 1 8 Direct Heat CO2 Incubator Thermo Scientific Access Port Filter amp C0 Sensor Cover Plate Air Sample Filter Installation Thermo Scientific l
51. e rinsing Wash the inner door both inside and out The cabinet and door must be rinsed with sterile water until the disinfectant has been removed After the cabinet has been rinsed spray with 70 alcohol 5 Install the left and right ducts inner door gasket access port filter assembly and air sample filter and tubing spraying each with 7096 alcohol 6 Install a new HETA filter 7 Install the shelves and spray with 7096 alcohol Clean Cabinet Clean the incubator exterior with a damp sponge or soft well wrung cloth Exterior and mild detergent dissolved in water Dry with a soft cloth 5 2 Direct Heat CO2 Incubator Thermo Scientific Section 5 Routine Maintenance Clean Glass Doors T e chamber glass door and the optional independent inner glass doors may be cleaned using the same disinfectant as used on the incubator interior t is imperative that they be rinsed vvith sterile distilled vvater to remove the disinfectant residue The doors should then be dried vvith a soft cloth Some precautions in the cleaning and care of the incubator glass doors Moisture leaches alkaline materials sodium Na from the surface of the glass Evaporation of the moisture concentrates the alkaline and may produce a white staining or clouding of the glass surface Cleaning chemicals with a PH above 9 and heat autoclaving accelerate the corrosion process Therefore it is very important to rinse and dry the glass doors after cleaning Auto
52. een CYC COMPLETE POWER OFF and REPLACE IR The white LED is lighted but no longer flashes Clean the IR sensor with either isopropanol or Lysol No Rinse Sanitizer When using isopropanol simply spray the sensor do not saturate and allow to dry With Lysol spray the sensor do not saturate and allow to sit for a couple of minutes Wipe dry with a clean soft cloth Do not saturate the sensor or immerse the sensor in a cleaner Power the unit off open the chamber and remove the IR plate Pull down on the dummy unscrew the cable from the top of the dummy and press the dummy into the clip on the plate See Figure 5 12 Screw the cable onto the top of the IR sensor The cable is keyed and can only be inserted one way Reinstall the sensor by pushing it as far as it will go into the hole After the unit is powered back on with the IR sensor reinstalled the display toggles between CYC COMPLETE REPL HEPAs and PRESS ENTER AUN SET CAL o wm 399 tn RH Z Se CYCCOMPLETE INJ CO Z A de IZI v RUM SET CRL CON o o o o 42 near 30 0 rra N RH Z REPLACE HEPA 155 INJ CO 4 A x 0 21 Sa HEAT 300 rra iw RUN SET CAL CON o o o a AH IZI iss mW co Z z Z Z PRESS ENTER Z Figure 5 18 IR Sensor Reinstalled Direct Heat C02 Incubator 5 11
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54. eplacement timer can be set for a specific amount of time from 1 to 12 months of actual unit running time Time will not accrue when the unit is turned off The default time is 6 months When the allotted time runs out REPLACE HEPA appears in the display and the visual alarm flashes To set the reminder use the following procedure 1 Press the MODE key until the CON indicator lights 2 Press the right arrow until REPL HEPA XX is displayed 3 Press the up down arrow to choose the number of months desired 4 Press ENTER to save the number 5 Press the MODE key to return to RUN mode or right left arrow to go to next previous parameter Note After the reminder has been set check the allotted time remaining by going to Configuration mode then pressing the right arrow until NEW HEPA XXX displays This number is the remaining days before the filter replacement time specified runs out For example if 12 months was chosen in the REPL HEPA XX message display the NEW HEPA number would be 365 days A A 3 digit Access Code can be entered to avoid unauthorized personnel from changing the setpoints calibration or configuration A setting of 000 bypasses the access code The factory setting is 000 1 Press the MODE key until the CON indicator lights 2 Press the right arrow until ACC CODE XXX shows in the message display 3 Press up down arrow to change the access code 4 Press ENTER to save the access code 5 Press the MODE key to
55. ey are operating properly Should an error be detected the incubator sounds an alarm and shows the appropriate message Contact your local distributor Thermo Scientific 10SU S 709 BY uo 1 BurAejds piony 8 e pazi eJeurulep JO p lihsip S S 950 e S010 U3 s Jn n3 JOJ pasapmod sn zou oq e sey yey 1ue329jursip Aue JO asn ONOG e s10jeqnaoui 2 10 sdi Aoejn32e pue suonipuoo jeyueuiuoJiAue asn uodn 1u pu d p SjBAJAJU JUaWISN pe uoneJqijeo pue Buiuea SAJIAJAS EJIUUJA 1981009 0 sanueleAA JO e2ueugjule N OMJUBA91J UO UOneulloJul JOJ Sgae AUeW ui lQP PAR SJUBUINIJSUI 9 qea2eJ ISIN Duisn sueraiuu2e1 aas poljijenb IM S E1J9p 8 JO enuew Bunei1 do au Jo uoes p lil ds y syueuigJinbaJ e2ueugjureui 10 JO SI pesuapuoo e si BUIMOJJOJ au UPI91UU29 3IAJ8S poljijenb e Ag Ajjeorpouad 63 pue payoauo aq JUN OU SI 11 AQUAIIIJJa pue 9JUEUJOJJAd UNUIXEW 104 siseq JEJNBAJ e uo e3ueuagjureui pue Bulue 3 unnol woad 10161800 su Auredoud Buruomnoun l n Jno d y 01 juejioduui SI e2ueugjureu 0 JEJNBAH 1u uidius pajeJqi eo pue pa1sa1 AjyHnoJoy seu juauidinba ino A sJOjeqnau 43NVN41NIVIN 3ALLN3A3Ud p p u se r 4 uoneziy 3s UIJOJIAJ
56. f the system detects an alarm condition see Section 4 Alarms The mes sage CLASS 100 is a timing mechanism indicating that under normal operating conditions vvith the HETA filter installed the air inside the chamber meets the Class 100 air cleanliness standard for particulates of 0 5 micron size or larger per cubic foot of air Further information on the Class 100 classification of air quality is available from Thermo Upper and Lovver Displays The 7 segment upper display shovvs temp and RH The lower display shows CO2 and 1 Maintain a minimum three inch clearance behind the incubator for electrical and gas hook ups In addition a three inch ventilation space is needed on each side 2 Locate the unit on a firm level surface capable of supporting the unit weight of 260 bs 118kg 3 Locate the unit away from doors and windows and heating and air conditioning ducts 4 Lift the unit by the sides of the cabinet base Do not attempt to lift it by the front and back This places stress on the outer door hinges Thermo Scientific Thermo Scientific Stacking the Incubators Section 1 Installation and Start Up Warning Install stacked units against a wall or similar structure A Warning With incubators in a stacked configuration do not leave both exterior doors open at the same time A Warning If the units have been in operation turn them both off and disconnect the power source before beginning any service
57. he air sample filter to the hose barb on the Filter chamber ceiling 2 Connect the other end of the filter to the metal tubing on the HEPA filter adapter Figure 5 2 Air Sample Filter Figure 5 2 Connect to Metal Tubing Electronics Section 1 Turn off the incubator s power switch and unplug the power cord 2 Remove the three screws from the back of the cabinet top 3 Slide the top backward about an inch and lift it off 5 4 Direct Heat CO2 Incubator Thermo Scientific Thermo Scientific Section 5 Routine Maintenance Major Components Refer to Figure 5 3 Some components shown are factory installed options Front of incubator Power DC power supply switch Fuse Analog Board i E 4 20m 0 14 0 5 Micro Board Blower Motor Component fan CO sensor p or TU EN thermostat Sample port vertemp thermostat into i RT RH board IR CO t NEN ERE sensor injection tubing Main power transformer o C gas solenoid Ed fan Filtered line corc T connector Back of incubator Accessory outlet 75W max C02 microbiological filter r 21 m Remote P alarm contacts connector and wire through put Figure 5 3 Electronics Drawer Direct Heat C02 Incubator 5 5 Section 5 Routine Maintenance Sterilization Cycle Information You Need to Know Before Starting a
58. hich may affect humidity levels in the chamber are the water level in the humidity pan frequency of door openings and the fact that the humidity pan is located on the bottom of the chamber versus on a shelf Caution Incubators equipped with a T C thermal conductivity CO2 sensor rely on a constant level of relative humidity to accurately measure and control the CO concentration in the incubator A Thermo Scientific Thermo Scientific Section 7 Specifications Temperature Control 0 1 C Microprocessor PID Control Setpoint Digital Touch pad 0 1 C Range 5 C above ambient to 50 C Uniformity 0 3 C 37 C Tracking alarm User programmable low indicator Overtemp Tracking user programmable action and indicator Display Digital LED 0 1 C increments Temperature Safety Type Extreme temperature safety action and indicator Sensor Thermostat independent of temp control system Indicator Message center audible and visual alarms Relative Humidity Control Humidity pan natural vaporization Humidity w pan 9506 RH at 37 C Display sus etre Optional in 196 increments Alarm Low RH with optional RH monitor CO Control 10 196 microprocessor PID control viste pad qi eda T C or IR Readabilip au dote iu eva Rees 0 196 RANGE PCs 0 to 2096 Inlet pressure sil o REESE cs 15 psig 1 bar Display
59. ht arrow until RH RLY XXX shows in the message display 3 Press up down arrow to toggle the setting ON OFF 4 Press ENTER to save the setting 5 Press the MODE key to return to RUN mode or right left arrow to go to next previous parameter Enabling Temp RH to onunits equipped vvith the RH option the upper seven segment display be Displayed on the control panel can be configured to display Temp continuously RH continuously or toggle between Temp and RH If the units does not have RH the upper display always shows temperature If temperature is set to ON and the RH is set OFF temperature shows continuously If temperature is set to OFF and RH is set to ON RH shows continuously If both are turned ON the display toggles between the two The factory setting defaults to toggle mode if the RH option is present 1 Press the MODE key until the CON indicator lights 2 Press the right arrow until DISP TMP XXX or DISP RH XXX shows in the message display 3 Press up down arrow to toggle the setting ON OFF 4 Press ENTER to save the setting 5 Press the MODE key to return to RUN mode or right left arrow to go to next previous parameter Thermo Scientific Direct Heat C02 Incubator 3 7 Section 3 Configuration Configure Mode Press MODE to light CONFIG Run Set Cal Config Heat Temp 2777 O O c l 36 9 4002 Scroll for Parameters ca To Configure Press MODE to return f Audible alarm ON to RUN mode
60. iation of temperature CO power NO and NC Thermo Scientific Thermo Scientific Section 7 Specifications Dimensions Exterior 26 1 W x 39 5 H x 25 0 F B MEET 66 3cm x 100 3cm x 63 5cm Interior 21 3 W x 26 8 H x 20 0 F B PENES 54 0cm x 68 1cm x 50 8cm Weight Ee SS 260 Ibs 118kg Net shipping 315 lbs 143kg Certifications Refer to the Declaration of Conformity at the end of this manual Safety Specifications Indoor Use Only Alutide xl x MET 2 000 meters Temperature 5 C to 40 C Humidity 80 RH at or below 31 C R decreasing linearly to 5006 RH at 40 C Mains Supply Fluctuations Mains supply voltage fluctuations not to exceed 10 of nominal voltage Installation Category II Pollution Degree 22 Class of Equipment 1 Installation category overvoltage category defines the level of transient overvoltage which the instrument is designed to withstand safely It depends on the nature of the electricity supply and its overvoltage protection means For example in CAT II which is the category used for instruments in installations supplied from a supply comparable to public mains such as hospital and research laboratories and most industrial laboratories the expected transient overvoltage is 2500V for a 230V supply and 1500V for a 120V supply 2 Pollution Degree describes the amount of conductive pollution present in the o
61. it Figure 5 4 for approximately 3 seconds until the LED lights Sterilization 9 Cycle Button Figure 5 4 Sterilization Button Location 3 a Nje lt gt IR sensor HEPA ENTER the access code if applicable An access code is recommended to prevent accidental cycle initiation Pre Sterilization with T C CO sensor The display toggles between REMOVE HEPAs REMOVE WATER and PRESS ENTER If ENTER is not pressed within 1 minute the display returns to SYSTEM OK Remove the HEPA filter air sample filter and access port filter assembly Figure 5 5 Tubing can remain in the unit during the sterilization cycle In addition remove the temp RH recorder probe and IR sensor if applicable Discard the filters Temp RH A recorder probe behind plate Filter Access Temp sensor Port Filter Air Sample inserted into andstopper Filter HEPA collar from assembly behind top duct Figure 5 5 Filter Locations Pre Sterilization with IR CO sensor If an IR sensor is connected the display toggles between POWER OFF and REMOVE TR If power is not turned off within 1 minute the display returns to SYSTEM OK Thermo Scientific Sterilization Cycle Thermo Scientific continued Section 5 Routine Maintenance Note To remove the IR sensor if applicabl
62. net This system is designed as a safety device to turn off all heaters in the event of a temperature control failure l emperature control in the incubator will be 1 of the overtemp setpoint The overtemperature is set by the factory default at 40 C However the overtemp can be set up to 55 C in 0 5 increments If the incubator s operating temperature setpoint is set above the overtemp setpoint the overtemp setpoint will automatically update to 1 C above the temperature setpoint It is recommended that the overtemp setpoint be maintained at 1 C over the operating temperature setpoint 1 14 Direct Heat C02 Incubator Thermo Scientific Setting the vertemp Setpoint continued Setting the CO Setpoint Thermo Scientific Section 1 Installation and Start Up To set the Overtemp setpoint 1 Press the MODE key until the SET indicator lights 2 Press the right arrow until Otemp XX X is displayed in the message display 3 Press the up or down arrow key until the desired Overtemp setpoint is displayed 4 Press ENTER to save the setting 5 Press the MODE key until the RUN indicator lights or press the right or left arrow to go to the next or previous parameter All T C CO cells are calibrated at the factory at 37 C high humidity and 1096 CO Therefore if a temperature setpoint of 37 C has been entered the humidity pan has been filled and the CO control is to run between 0 10 with a T C CO sensor the
63. nit itself For equipment service or maintenance or with technical or special application inquiries contact your local distributor Repairs NOT Covered Under Warranty Calibration of control parameters Nominal calibrations are performed at the factory typically 1 C for temperature 1 for gases and 5 for humidity Our service personnel can provide precise calibrations as a billable service at your location Calibration after a warranty repair is covered under the warranty Damage resulting from use of improper quality water chemicals or cleaning agents detrimental to equipment materials Service calls for improper installation or operating instructions Corrections to any of the following are billable services electrical service connection 2 tubing connections 3 gas regulators 4 gas tanks 5 unit leveling 6 room ventilation 7 adverse ambient temperature fluctuations 8 any repair external to the unit Damage resulting from accident alteration misuse abuse fire flood acts of God or improper installation Repairs to parts or systems resulting from unauthorized unit modifications Any labor costs other than that specified during the parts and labor warranty period which may include additional warranty on CO sensors blower motors water jackets etc Thermo Scientific Direct Heat C02 Incubator V Thermo Scientific Table of Contents Installation and Start Up
64. ogrammed to trip the remote alarm Trip Contacts contacts A setting of ON allows this a setting of OFF blocks CO alarms from tripping the contacts The factory setting is ON 1 2 Press the MODE key until the CON indicator lights Press the right arrow until CO2 RLY XXX shows in the message display Press up down arrow to toggle the setting ON OFF Press ENTER to save the setting Press the MODE key to return to RUN mode or right left arrow to go to next previous parameter Set New Zero Number new T C CO sensor is being installed the two numbers on the for T C CO Sensors factory installed sticker on the T C cell must be entered to calibrate CO2 in the unit Note For the technician s convenience a label containing the two numbers on the T C cell is affixed inside the electronics drawer A 1 2 Thermo Scientific Press the MODE key until the CON indicator lights Press the right arrow until T CZ XXXX shows in the message display Press up dovvn arrovv to change the zero number to match the sticker Press ENTER to save the setting Press the MODE key to return to RUN mode or right left arrovv to go to next previous parameter Direct Heat C02 Incubator 3 5 Section 3 Configuration Set New Span Number for T C CO Sensors Setting a Low RH Alarm Limit 3 6 Direct Heat C02 Incubator If a new T C CO sensor is being installed the two numbers on the factory installed sticker on the T C cell m
65. perating environment Pollution degree 2 assumes that normally only non conductive pollution such as dust occurs with the exception of occasional conductivity caused by condensation Direct Heat C02 Incubator 7 3 Section 8 Spare Parts Part Number Description 100000 geen Removable feather gasket 190670 Duct sheet and shelf channel kit 224175 s s RS AME Stainless steel shelf 190884 Stainless steel shelf and channels 191650 cee ase ee Micro board main control 230135 Fuse 1A TD 5 x 20mm accessory outlet 115V 230120 Fuse 0 5A TD 5 x 20mm accessory outlet 230V 490109 i5 soo Eee ot Cordset 10A 250V Tl s Motor 2 pole 115VAC 50 60 Hz 100202 Display board 290184 Probe 2252 Ohm 25 C 1 8 x2 150072 op Stopper vvith 3 8 hole 290168 Thermal conductivity T C type CO2 sensor 2912045 1 int eio TRO ee Infrared type CO2 sensor 2270112 Lose ted CO2 valve kit with assorted fittings 963010 o roue so wer Vague oon SEEK CO2 gas regulator 21690242 C AE Gas shuttle switch 3030 51 0 External gas guard UE Wall clamp for CO2 bottle 231203 RH sensor assembly P N 1900091 RH display option 230180 12A DPDT circuit breaker switch power 400119 Svvitcher 40V7 12 5 12V 196018 34 position control to display ribbon cable 227011 see rp iz Inner door svvitch 93
66. r to save setting Numbers decrease Numbers increase Press Enter to save setting Numbers decrease 1 16 Direct Heat C02 Incubator Thermo Scientific Thermo Scientific Temperature Section2 Calibration After the unit has stabilized several different systems can be calibrated In Calibration mode the air temperature CO2 and RH levels can be calibrated to reference instruments To access Calibration mode press the MODE key until the CAL indicator lights Press the right and or left arrow until the appropriate parameter appears in the message display See Chart 2 1 at the end of this section for more detail Calibration frequency is dependent on use ambient conditions and accuracy required A good laboratory practice would require at least an annual calibration check On new installations all parameters should be checked after the stabilization period Prior to calibration the user should be aware of the following system functions While the unit is in Calibration mode all system control functions are stopped so the unit remains stable Readout of the system being calibrated appears on the message display If no keys are pressed for approximately five minutes while in Calibration mode the system resets to RUN mode so control functions are reactivated Caution Before making any calibration or adjustments to the unit it is imperative that all reference instruments be properly calibrated A Before calibration allow the
67. r TEMP Low Tracking Alarm RH is lower than RH Low Limit Alarm RH option All alarm delays and ringback times are 30 seconds Temperature Controller Failure See Sect 4 TMP CTRL ERR Omm pn Im OO es No ES No No No When multiple alarm conditions occur active messages are shown in the message display one at a time updating at 5 second intervals Pressing fe during multiple alarms causes all active alarms to be silenced and to ring back in 15 minutes The TEMP IS LOW alarm is disabled when the Temp set point is 10 C The CO alarms are disabled when the CO set point is 0 0 Thermo Scientific Direct Heat C02 Incubator 4 1 Section 4 Alarms Temp Controller Failure 4 2 TMP CNTR ERR Sensor Fault Alarms Direct Heat C02 Incubator In addition to other safety features designed into Series 8000DH incubators a thermostat is provided to monitor the cabinet s temperature In the unlikely event of a temperature control failure the thermostat will turn off all heaters at a cabinet temperature of 160 C 5 This is intended to be a safety feature to protect the incubator and is not intended to protect the cell cultures or the equipment inside the chamber should a temperature control failure occur Should such a failure occur contact the Technical Services Department or your local distributor The microprocessor in Series 8000DH incubators continually scans all available sensors to ensure that th
68. rm The outer door is open longer than 20 seconds during phases listed above Cycle is canceled unit goes to CANCELED COOL PHASE The outer door is open during the cool down phase when the temp is 60 C or greater An outer door alarm occurs Power interruption during the HEAT PHASE HEAT PHASE resumes if the chamber temperature was less than 90 C when power was interrupted HEAT PHASE resumes if the chamber temperature was greater than 90 C when power was interrupted and the temperature dropped less than 1 C CANCELED COOL PHASE starts if the chamber temp drop is more than 1 C Power interruption during the STERILIZATION PHASE HEAT PHASE begins if the chamber temperature has not dropped below 139 C STERILIZATION PHASE starts again when the chamber temperature reaches 140 C CANCELED COOL PHASE starts if the chamber temp dropped below 139 C For further information on CANCELED COOL PHASE see page 5 12 Thermo Scientific Direct Heat C02 Incubator 5 7 Section 5 Routine Maintenance 5 8 Sterilization Cycle Direct Heat C02 Incubator Warning The Sterilization Cycle heats the incubator interior surfaces to 140 C Contact with any surface inside the outer door during this cycle may result in burns A Empty the vvater from the humidity pan and place the pan back into the incubator Remove any samples instruments dishes etc from the chamber Press and hold the large white sterilization cycle button on the right side of the un
69. s Calibration and Configuration Runis the default mode for normal operation Set is used to enter system setpoints for incubator operation Calibration is used to calibrate various system parameters Configuration allows for custom setup of various options Table 1 1 Selections Under Each Mode RUN SETPOINT CALIBRATION CONFIGURATION Default Mode Temperature Temp Offset Audible Overtemp C0 tal Nevv HEPA Timer C0 IR Cal Replace HEPA Reminder RH Cal Access Code Temp Lo Alarm Temp Relay 02 Lo Alarm C0 Hi Alarm C0 Relay C02Z amp S s RH Lo Alarm RH Relay Display Temp Display RH T C units Base Unit Displays IR units Option Unit Displays T C units only Direct Heat C02 Incubator 1 3 Section 1 nstallation and Start Up Keypad Operation continued Displays Installing the Incubator 1 4 Direct Heat C02 Incubator SCROLL FOR PARAMETERS Arrows Steps the operator through parameters of SET CAL and CON modes The right arrow goes to the next parameter the left arrow returns to the previous parameter Up Down Arrows Increases decreases or toggles the parameter value selected in SET CAL and CON modes ENTER Must press this key to save to memory all changed values Key Press to mute audible alarm See Section 4 for alarm ringback times Message Display Shows system status Mode at all times Shows CLASS 100 or SYSTEM OK during normal operation or alarm messages i
70. s compliance with this directive the recyclers in your country and information on Thermo Scientific products will be available at www thermofisher com Marking of electrical and electronic equipment which applies to electrical and electronic equipment falling under the Directive 2002 96 EC WEEE and the equipment that has been put on the market Sa v Always use the proper protective equipment clothing gloves goggles etc v Always dissipate extreme cold or heat and wear protective clothing v Always follow good hygiene practices v Each individual is responsible for his or her own safety Thermo Scientific Direct Heat CO2 Incubator iii Preface Do You Need nformation or Assistance on Thermo Scientific Products f you do please contact us 8 00 a m to 6 00 p m Fastern Time at 1 740 373 4763 Direct 1 800 438 4851 Toll Free U S and Canada 1 877 213 8051 FAX http www thermoscientific com Internet Worldwide Web Home Page service led marietta thermofisher com Tech Support Email Address www unitylabservices com Certified Service Web Page Our Sales Support staff can provide information on pricing and give you quotations We can take your order and provide delivery information on major equipment items or make arrangements to have your local sales representative contact you Our products are listed on the Internet and we can be contacted through our Internet home page Our Service Support staff can supply technical informa
71. s may occur For example 5 and tubing stainless steel turns a stravv color after a period m of exposure to high temperatures This is normal 5 6 Direct Heat C02 Incubator Thermo Scientific Section 5 Routine Maintenance Checkpoints What if Then Cycle does not initiate or terminates in mid cycle Check for alarms SYS IN OTEMP AIR SNSR ERR TMP CTRL ERR Units are stacked A Sterilization Cycle performed on either unit affects performance on second unit Do not use second unit during Sterilization Cycle The Sterilization Cycle may be performed on both units simultaneously with proper electrical connection No action is taken within 1 minute following display prompt Unit returns to normal operation SYSTEM OK Need to cancel cycle in progress Hold down green cycle initiation button for 3 seconds Water is not removed from humidity pan during cycle Steam is produced and may cause burns Listed components are not removed before initiating cycle Components cannot withstand the sterilization cycle temperatures and are destroyed Unit is not powered off before removing the I R sensor if applicable Damage to the sensor may result Dummy sensor is not installed The sensor cable cannot withstand sterilization cycle temperatures and is destroyed properly The outer door is opened during the heat or sterilization phases An outer door alarm occurs CLOSE DOOR in the display plus an audible cannot be silenced and visual ala
72. setting Display Humidity Sai amem Press to return Display humidity OFF to previous parameter Tank 1 prime Press Enter to Tank Select save the setting Press to return O Tank 2 prime to previous parameter Gas Guard ON Gas Guard GASGRD XXX sun Scroll for Parameters Press to return Gas Guard OFF to previous parameter Numbers increase Press Enter to Ent RS 485 XX save the RS485 address Press to return 2122 to previous parameter umbers decrease Chart3 1 3 cdr 3 10 Direct Heat C02 Incubator Thermo Scientific Section 4 Alarms The Series 8000DH incubator alarm system is shovvn in the table belovv VVhen an alarm is active the message appears in the LED message display Pressing Y disables the audible alarm for the ringback period However the visual alarm continues until the incubator returns to a normal condition The alarms are momentary alarms only When an alarm condition occurs and then returns to normal the incubator automatically clears the alarm condition and the message display Table 4 1 Alarm and Description No alarm condition exists SYSTEM OK or CLASS 100 m n Temp 5 Otemp Set point SYS IN OTEMP Y Air Temp Sensor Fault See Section 4 AIR SNSR ERR Y C02 Sensor Fault See Section 4 C02 SNSR ERR Replace HEPA filter reminder set time expired REPLACE HEPA C02 is higher than CO2 High Tracking Alarm C02 is lower than CO2 Low Tracking Alarm TEMP is lowe
73. sor with flammable solutions The internal temperature of the CO sensor is approximately 150 C when the unit is in operation Allow sufficient time for the sensor to cool before cleaning Do not use strong alkaline or caustic agents Stainless steel is corrosion resistant not corrosion proof Do not use solutions of sodium hypochlorite bleach as they may also cause pitting and rusting A A HETA filter replacement kit see Parts List should be on hand prior to cleaning the incubator interior Direct Heat C02 Incubator 5 1 Section 5 Routine Maintenance Clean Chamber 1 Remove the shelves access port filter assembly HEPA filter air sample Interior filter and tubing and the left and right duct sheets Discard the HEPA filters and the access port filter assembly See Figure 5 1 e i l HEPA d Access Filter N Air Sample Port Filter Filter and stopper and tubing d assembly Humidity Pan Figure 5 1 Component Locations 2 Wash the air sample filter tubing shelves and ducts with disinfectant then rinse with sterile water Optionally the shelves and ducts may be autoclaved 3 Wash the inner door gasket with disinfectant This gasket may be removed to be cleaned or replaced 4 Wash the cabinet interior with disinfectant starting at the top and working down Refer to the disinfectant directions for length of time needed befor
74. t operate the unit without the HETA filter in place A 1 Remove the filter from the shipping box 2 Remove the plastic coating from the filter using caution not to touch the filter media 3 Install the filter as shown in Figure 1 18 Refer to Section 5 for HEPA filter maintenance Caution The media of the filter can be damaged if mishandled To avoid damage to the incubator do not operate the unit without the HETA filter in place A Check the unit for being level by placing a bubble style level on one of the shelves Turn the hex nut on the leveler counterclockvvise to lengthen the leg or clockwise to shorten it Level the unit front to back and left to right See the serial tag on the side of the unit for electrical specifications or refer to the electrical schematics at the end of this manual Caution Serial tag rating is based on amperage draw during sterilization cycle Normal operating amperage is much less Ensure that electrical circuit will handle amp draw of sterilization cycle Connect the incubator to a grounded dedicated circuit The power cord connector is the mains disconnect device for the incubator Position the unit so that it can be easily disconnected A Thermo Scientific Section 1 nstallation and Start Up Electrical Povver Plug the provided povver cord into the povver inlet connector on the back Connection co nt of the cabinet Figure 1 19 then into the grounded dedicated electrical circuit
75. ter Models 3542 and 3543 have an infrared IR CO sensor Infrared CO sensors are not effected by chamber atmosphere temperature or humidity However the light detector in the sensor is effected by wide temperature changes Therefore changing temperature setpoints could necessitate a recalibration of the CO Chamber temperature should be allowed to stabilize before checking CO concentrations with an independent instrument especially on start up IR CO Sensor Stabilization Times Start Up Allow the temperature and the CO of the cabinet to stabilize at least 12 hours before proceeding Presently Operating Allow CO to stabilize at least 2 hours at setpoint before proceeding Direct Heat C02 Incubator 2 3 Section 2 Calibration 24 Infrared CO System continued Relative Humidity Direct Heat C02 Incubator Measure the CO concentration in the chamber through the gas sample port with a Fyrite or other independent instrument Several readings should be taken to ensure accuracy 2 Press the MODE key until the CAL indicator lights 3 Press the right arrow until IR CAL XX X appears in the message display 4 Press the up down arrow to adjust the display to match the independent instrument reading 5 Press ENTER to store the calibration 6 Press the MODE key to return to RUN mode Model 3540 and 3541 incubators are equipped with an optional direct readout relative humidity sensor This is
76. the brackets with the screws and washers provided in the parts bag See Figure 1 8 e eS APIS b Uni Stacking Stacking Bottom incubator back Ne RR Ku Figure 1 7 Bracket Figure 1 8 Installed Brackets on Back of Unit into Slot 7 Thread one 1 4 x 20 bolt and washer included vvith the stacking brackets Cover plate and bolt hole into the hole behind the cover plate sith MOOR eoniaetor Do not tighten Refer to Figure 1 9 BE 8 Remove the cover plate from the same area on the other side of the top unit Figure 1 9 Bolt Hole 9 Thread the other 1 4 x 20 bolt and washer into this hole 10 Tighten the bolts on both sides 11 Assemble the door hinges to the unit Secure with the screws 12 Plug the door cord into the connector as previously Secure the cover plate 13 Install the cover plate on the other side of the unit The stacked incubators are ready for service 1 Remove vinyl from shelf channels duct sheets and air duct if present 2 Using a suitable laboratory disinfectant clean all interior surfaces Caution Before using any cleaning or decontamination method except those recommended by the manufacturer users should check with the manufacturer that the proposed method will not damage the eguipment Accidental spills of hazardous materials on or inside this unit are the responsibility of the user A Thermo Scientific Section 1 nstallation and Start Up D
77. through an aging period especially on new installations Calibration should be checked on a weekly basis and adjusted as necessary When stabilization occurs checks can become less frequent T C CO Sensor Stabilization Periods Start Up The CO sensor has been calibrated at the factory for 37 C and elevated humidity Allow the temperature humidity and CO levels in the chamber to stabilize at least 12 hours before checking the CO concentration with an independent instrument Presently Operating Make sure the chamber doors are closed Allow at least 2 hours after the temperature and CO displays reach their setpoints for chamber atmosphere stabilization 2 2 Direct Heat CO2 Incubator Thermo Scientific Thermal Conductivity CO System continued Infrared CO System Thermo Scientific Section 2 Calibration Make sure the stabilization periods outlined above are follovved Sample the chamber atmosphere through the sample port vvith an independent instrument Sample the atmosphere at least 3 times to ensure accuracy of the instrument 3 Press the MODE key until the CAL indicator lights 4 Press the right arrow until CO2 CAL XX X is displayed in the message display 5 Press the up down arrows to change the display to match the independent instrument 6 Press ENTER to store the calibration 7 Press the MODE key to return to RUN or the right or left arrows to go to the next previous parame
78. tific Thermo Scientific Section 6 Section 7 Section 8 Section 9 Section 10 Table of Contents Factory Options 6 1 Remote Alarms xd aaa aka 6 1 Humidity Readout a Seabees asus 6 2 UE TEE 7 1 Spare Parts uu aun da dy aa Sen deese tea Ue 8 1 Exploded Part Drawings Eu A lab bends 8 2 Electrical Schematics 9 1 Warranty Information 10 1 Direct Heat C02 Incubator viii Section 1 nstallation and Start Up Chamber Gas Sample Port Power y Switch Control Panel vA See et Section 1 Lo Sterilization Cycle Button Inner Door m 2 Z sN Door Heater Cable Figure 1 1 Components See Section 5 Leveling lt Legs 4 e Chamber Gas Sample Port Used for sampling chamber CO content using a FYRITE or similar instrument e Main Power Switch Mains Disconnect Control Panel Keypad displays amp indicators Figure 1 2 Leveling Legs Used to level the unit Sterilization Cycle Button Switch to initiate sterilization cycle Note The incubators are stackable Information follows A Thermo Scientific Direct Heat C02 Incubator 1 1 Section 1 nstallation and Start Up Vf Mutes the audible alarm
79. ting C0 Gas Warning If higher purity CO is desired inside the incubator greater than 99 596 pure the pressure regulator should be constructed with a stainless Supply continued steel diaphragm along with specifying the purity of the CO from the gas supplier Follow the manufacturer s instructions to ensure proper and safe installation of the pressure regulator on the gas cylinder Consult your facility safety officer to ensure that the equipment is installed in accordance with the codes and regulations that are applicable in your area A The CO gas supply being connected should be industrial grade 99 596 pure and should not contain siphon tubes Install a two stage pressure regulator at the cylinder outlet The high pressure gauge at the tank should have 0 2000 psig range The low pressure gauge at the incubator inlet should have a 0 30 psig range Input pressure to the incubator must be maintained at 15 psig 103 4 kPa The incubator has serrated fittings on the back of the cabinet to connect the gas supply Refer to Figure 1 19 The fitting is labeled CO2 Inlet 1 Tank Make sure that the connections are secured with clamps Check all fittings for leaks Incubator Sta rt Up With the incubator properly installed and connected to power the humidity pan filled and the unit connected to gas supplies system setpoints can be entered The following setpoints can be entered in SET mode Temperature Overtemperature and CO To enter
80. tion about proper setup operation or troubleshooting of your equipment We can fill your needs for spare or replacement parts or provide you with on site service We can also provide you with a quotation on our Extended Warranty for your Thermo Scientific products Whatever Thermo Scientific products you need or use we will be happy to discuss your applications If you are experiencing technical problems working together we will help you locate the problem and chances are correct it yourself over the telephone without a service call When more extensive service is necessary we will assist you with direct factory trained technicians or a qualified service organization for on the spot repair If your service need is covered by the warranty we will arrange for the unit to be repaired at our expense and to your satisfaction Regardless of your needs our professional telephone technicians are available to assist you Monday through Friday from 8 00 a m to 6 00 p m Eastern Time Please contact us by telephone or fax If you wish to write our mailing address is Thermo Fisher Scientific 401 Millcreek Road Box 649 Marietta OH 45750 International customers please contact your local Thermo Scientific distributor V Direct Heat C02 Incubator Thermo Scientific Preface VVarranty Notes nformation You Should Know Before Requesting Warranty Service Locate the model and serial numbers A serial tag is located on the u
81. uct Sheet and Shelf 1 Installed included grommets inot the back Installation flange of each duct sheet 6 grommets per sheet See Figure 1 10 2 Install the side ducts with the tabs facing into the center of the chamber vvith their slots There are no right side or left side ducts simply rotate one of them to fit the opposite side Tilt the side ducts as they are placed into Figure 1 10 Grommets the chamber so the tops fit into the top air on back of duct sheet duct then guide them into the vertical position Figure 1 11 shows the side duct as it would be oriented for the right side of the chamber fi Side toward side duct Shelf Channels CeT Side toward shelf Shelf channel Side duct Shelf channel rear slot tab front slot Side duct tab Right Side Duct with Shelf Channel Installed Figure 1 11 Shelf Channels amp Side Ducts Figure 1 12 Channels and Slots unuuununununuu nunannunnaunaunu Side Duct 3 Referring to Figure 1 11 note that there is no difference in the left and right shelf channels 4 Install the shelf channels by placing the channel s rear slot over the appropriate rear tab on the side duct Pull the shelf channel forward and engage the channel s front slot into the side duct s appropriate forward tab Refer to Figure 1 12 5 Figure 1 11 shows on
82. urposes only The contents and the product it describes are subject to change without notice Thermo Fisher Scientific makes no representations or warranties with respect to this manual In no event shall Thermo be held liable for any damages direct or incidental arising out of or related to the use of this manual Warning If the incubator is not used in the manner specified in this operating manual the protection provided by the equipment design may be impaired A 2007 Thermo Scientific All rights reserved Direct Heat CO2 Incubator Thermo Scientific Preface mportant operating and or maintenance instructions Read the accompanying text carefully w Potential electrical hazards Only qualified persons should perform procedures associated with this symbol CES DN di Eguipment being maintained or serviced must be turned off and locked off to prevent possible injury gt damaged by elevated temperatures Hot surface s present which may cause burns to unprotected skin or to materials which may be after 13 August 2005 This product is required to comply with the European Union s Waste Electrical amp Electronic Equipment WEEE Directive 2002 96 EC It is marked with the WEEE symbol Thermo Fisher Scientific has contracted with one or more recycling disposal companies in each EU Member State European Country and this product should be disposed of or recycled through them Further information on Thermo
83. ust be entered to calibrate the CO in the unit Note For the technician s convenience a label containing the two numbers on the T C cell is affixed inside the electronics drawer A 1 Press the MODE key until the CON indicator lights 2 Press the right arrow until T CS XXXX shows in the message display 3 Press up down arrow to change the span number to match the sticker 4 Press ENTER to save the setting 5 Press the MODE key to return to RUN mode or right left arrow to go to next previous parameter On units with the RH option installed a low RH alarm limit may be entered The low RH alarm limit is the RH in the cabinet that causes a low RH alarm The setpoint is variable from setpoint 0 to 90 RH The factory setting is 0 RH which disables the alarm 1 Press the MODE key until the CON indicator lights 2 Press the right arrow until RH LO XX shows in the message display 3 Press up down arrow to change the RH low alarm limit 4 Press ENTER to save the RH low alarm limit 5 Press the MODE key to return to RUN mode or right left arrow to go to next previous parameter Thermo Scientific Section 3 Configuration Enable RH Alarms to The low RH alarm can be programmed to trip the remote alarm contacts prog P Trip Contacts A setting of ON allows this a setting of OFF blocks the RH alarm from tripping the contacts The factory setting is ON 1 Press the MODE key until the CON indicator lights 2 Press the rig
84. vailable in Configuration mode allow custom setup of the incubator These features are listed with descriptions below All features may not be necessary in all applications but are available if needed To enter Configuration mode press the MODE key until the CON indicator lights Press the right and or left arrow until the appropriate parameter appears in the message display See Chart 3 1 for more detail The audible alarm can be turned on or off The factory setting is ON 1 Press the MODE key until the CON indicator lights 2 Press the right arrow until AUDIBLE XXX shows in the message display 3 Press the up down arrow to toggle AUDIBLE ON OFF 4 Press ENTER to save the setting 5 Press the MODE key to return to RUN mode or right left arrow to go to next previous parameter When the REPLACE HEPA reminder displays and the visual alarm flashes the specified time has elapsed and the HEPA filter should be replaced To clear the display and reset the timer after replacing the HEPA filter with a new one follow the steps below 1 Press the MODE key until the CON indicator lights 2 Press the right arrow until NEW HEPA shows in the message display 3 Press ENTER to restart the timer and clear the REPLACE HEPA alarm 4 Press the MODE key to return to RUN mode Direct Heat C02 Incubator 3 1 Section 3 Configuration 3 2 Set REPLACE HEPA filter reminder Set Access Code Direct Heat CO2 Incubator A HEPA filter r

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