Home

Upright Microscope ECLIPSE Ni-U Instruction Manual

1. There is no power even though the power switch is on The power cord is not connected or is connected improperly Connect the cable properly See Chapter 1 3 Assembly Method 20 Connect the motorized device cable in the Assembly Maintenance A motorized accessory does not operate A contact arm is not used Use a contact arm See Chapter 1 3 Assembly Method 5 Attach the standard arm contact arm in the Assembly Maintenance The motorized unit connected to the contact arm is attached incorrectly Attach the motorized unit correctly See Chapter 1 3 Assembly Method in the Assembly Maintenance The motorized unit connected to contact arm is not in correct combination Attach the motorized unit in the correct combination gt See Chapter 1 3 Assembly Method Introduction in the Assembly Maintenance Contact arm LED is blinking The motorized unit connected to contact arm is not in correct combination Attach the motorized unit in the correct combination gt See Chapter 1 3 Assembly Method Introduction in the Assembly Maintenance a Illumination Lamp does not light There is no power supplied Plug in the power cord See Chapter 1 3 Assembly Method 21 Connect the power cord in the Assembly Maintenance The lamp has burned out Replace the lamp with the
2. 0 eee ee eee ects eter teeeeeeeeaee 9 m Attaching a light shielding plate cece eee eeee teeter eeeeeeenaes 9 7 Attach the quadrocular tilting tube and DSC zooming port for quadrocular UE cacon terra dada aras 10 8 Attach the EPI motorized shutter optional 9 Attach Cye pieCes s sc cccsccsu hen cece dl 10 Attach the SUbStage ooococnnccinnccionncocccnonnconnccnnccnnnnnn 11 Attach the condenser holder ooococioccccccccccccoccconccconccinnnnns 12 Attach the Stage ooococconocconoccconcconccconccnnncnnancnonn conc conc ccnnnnnns 12 1 For standard stage cooocoiocccncccnccccocccinncnos 12 2 For rotatable ceramic coated stage 13 Attach the condenser civic m Attaching the optical module to the universal condenser m Attaching the DIC module to DIC condenser Oil ooononnnnicnnnnninnacanancno 14 Attach the NOSepiece ooooooocconccciocccocccconnncnncnnnncnononcnnc nn nc nn nc nan cnrn nc anar c nc nnn nan 15 Attach the ObjeCtiVO cocaina ed 19 16 Attach the DIC rotation polarizer unit required for differential interference CONTTASt MICKOSCOPY cinco cid ici 19 17 Attach the eyelevel riser Optional ccccceeeceeeeeeeeeeeceeeeeeeeeeeeseaeeeeeeeeeeeeeaes 20 18 Attach a camera and connect the DS camera control unit optional 20 18 1 When attaching to the DSC port for the ergonomic binocular tube 20 18 2 When attaching to the DSC zooming port for quadrocular tube
3. Pollution degree Degree 2 Installation Category II Electrical shock protection class Class Indoor use only Transport storage conditions Temperature 20 C to 60 C Humidity 90 RH max no condensation External dimensions and weight External dimensions 93 W x 160 H x 220 D mm excluding projections Weight 2 2 kg m Simple Remote Control Pad for Nikon Microscope Model NI SRCP Operating conditions Temperature 0 C to 40 C Humidity 60 RH max no condensation Altitude 2000 m max Pollution degree Degree 2 Installation Category II Electrical shock protection class Class Indoor use only Transport storage conditions Temperature 20 C to 60 C Humidity 90 RH max no condensation External dimensions and weight External dimensions 76 W x 20 H x 120 5 D mm excluding projections Weight 0 2 kg 44
4. Nikon Upright Microscope CLIPS Nz U Instruction Manual Assembly Maintenance M570 E 115 351 9 2 iquiassy Bunooys jqnosL abe10j S 9IULUIJUILIA suoyeoyiads pue Introduction Introduction Thank you for purchasing a Nikon product This instruction manual is written for users of the Nikon ECLIPSE Ni U microscope To ensure correct usage read this manual carefully before operating this product e No part of this manual may be reproduced or transmitted in any form without prior written permission from Nikon e The contents of this manual are subject to change without notice e The equipment described in this manual may differ from the actual product in its appearance e Although every effort has been made to ensure the accuracy of this manual errors or inconsistencies may remain If you note any points that are unclear or incorrect please contact your nearest Nikon representative e Some of the equipment described in this manual may not be included in the set you have purchased e If you intend to use any other equipment with this product read the manual for that equipment too e If this equipment is used in a manner not specified by the manufacturer the protection provided by the equipment may be impaired e Training This product can be used without special training provided that this manual is read thoroughly before use Kindly contact your nearest Nikon representative if you have any questions
5. See Chapter 3 4 Adjusting the Diopter in the Operation Eyes become fatigued Diopter adjustment has not been performed Perform diopter adjustment gt See Chapter 3 4 Adjusting the Diopter in the Operation Brightness is inadequate Adjust the brightness using the brightness control knob or ND filters to attain a suitable brightness See Chapter 3 1 Adjusting the Brightness of a Diascopic Image in the Operation The specimen does not move smoothly The specimen holder is not securely fixed to the stage Fix the holder securely See Chapter 2 2 Bright Dark field Microscopy 7 Place a specimen on the stage and move the stage to bring the target into view in the Operation Rotating torque of the stage knob is set too heavy Adjust to the appropriate torque weight See Chapter 3 3 Bringing the Target into the Optical Path Adjusting the knob rotation torque in the Operation Difficult to focus Rotating torque of the coarse focus knob is set too heavy Adjust to the appropriate torque weight gt See Chapter 3 2 2 Focus Knobs on the Main Body Adjusting the rotating torque of the coarse focus knob in the Operation The optical path does not switch when the quadrocular tilting tube is used The quadrocular tilting tube is fastened Unfasten it Remove the tightening screw for the qu
6. 100W OSRAM HLX 64623 Aim Standard arm NI SAM Combine with a manual model such as manual nosepiece Contact arm NIU CAM Combine with a motorized models such as intelligent motorized nosepiece Binocular tube C TB C TBM mildew proof type also available Trinocular eyepiece tube F C TF C TFM mildew proof type also available Tube Trinocular eyepiece tube T C TT Ergonomic tube C TE2 Quadrocular tilting tube NI TT DSC port DSC port for ergonomic binocular tube C TEP2 Connected to Nikon DS camera head or other C mount camera Nikon DS camera DSC zooming port for quadrocular tube NI RPZ head is controlled from DS L3 DS U3 CFI eyepiece GFI Eyepiece CFI UW Centering telescope C CT Universal quintuple nosepiece L NU5 BD quintuple nosepiece L NBD5 Sextuple nosepiece C N Sextuple nosepiece with analyzer slot C NA Nosepiece Sextuple DIC nosepiece D ND6 Intelligent DIC sextuple nosepiece NI ND6 I Intelligent Septuple nosepiece NI N7 I Motorized DIC sextuple nosepiece NI ND6 E Motorized septuple nosepiece NI N7 E CFI i A eer peer 5 LU Plan Fluor Epi BD can be attached to the L NU5 or L NBD5 nosepiece Objective CF objective LU Plan Fluor Epi LU nosepiece aapier is required to attach a non BD objective to LNBD5 LU Plan Fluor BD Substage Substage NI SS Rotatable ceramic coated stage can not be attached Substage for rotatable ceramic coated stage NI SSR Right handle stage with 2S holder C SR2S Left handle stage with 2S holder C SL2S Right handle ceramic coated
7. 11 Attach the condenser holder Attach a condenser holder to the substage Remove or attach the condenser holder after detaching the stage Tool Hex wrench 3 mm across flats Hook the half dovetail of the condenser holder onto the half dovetail groove of the lower substage and push it a holder IxIng screw against the reference surface Then tighten the fixing screw on the top of the substage Half dovetail Half dovetail groove Attaching the condenser holder 1 Turn the coarse focus knob until the substage is brought to the lowermost position 2 Loosen the stage clamp screw on the front of the stage 3 Align the stage with the round dovetail of the Stage substage and secure it in place with the stage clamp clamp screw screw do Securing the standard stage 122 For rotatable ceramic coated stage Tools Hex driver 2 mm across flats Centering tool Ball point hex drivers provided with the product x2 1 Sufficiently loosen the two right and left centering screws on the back of the substage and the stage positioning screw on the front of the stage using a hex driver 2 Attach by engaging the round dovetail at the bottom of the stage with the round dovetail of the substage 3 Center the stage and then tighten the stage positioning screw See Chapter 3 3 Bringing the Target into the Optical Path Horizontal Stage Movement Rotation mCentering the rotatable ceramic c
8. For DS L3 a USB cable can also be used for connecting the USB H connector for DS L3 and the USB connector for the connector box B 0 When connecting the camera trigger cable with the DSC connector be sure to insert it completely Precautions for connecting the camera trigger cable Tool Prior to photomicrography adjust the camera position and adjust the focus on the monitor as appropriate See Chapter 3 18 Capturing Images Photomicroscopy in the Operation Hex driver 2 mm across flats Attach the tube adapter for quadrocular tube to the quadrocular tilting tube and tighten the two fixing screws Attach a C mount camera an ENG mount camera or a photomicrography device via each adapter to the trinocular tube adapter Attach a C mount camera an ENG mount camera or a photomicrography device via an adapter to the trinocular tube 21 When attaching to the DSC zooming port for quadrocular tube Camera connection connector Camera head C mount N Attaching a camera to the DSC zooming port for quadrocular tube When attaching to the quadrocular tilting tube Tube adapter for quadrocular tube fixing screw Camera connection connector Camera head Tube adapter for quadrocular tube Attaching the tube adapter for quadrocular tube When attaching a camera to the trinocular tube Ajquiessy Ajquiessy Chapter 1 Assembly 19 Replace the ND f
9. L3 U3 is required To connect the USB H on the DS L3 to the USB on the control box B a USB cable is required To connect the EXT I O on the DS L3 U3 to the DSC on the microscope or the Ex OUT on the control box B the C CTC camera trigger cable L3 U3 is required To connect the motorized shutter to the EPI SHUTTER or DIA SHUTTER on the control box B the NI SHCL motorized shutter cable long is required To connect the RS232C on the C HGFIE motorized HG precentered fiber illuminator to the HGFIE on the control box B an RS232C cross wired cable is required commercial D Sub9 pin female for both connectors Q Precautions for connecting the camera trigger cable When connecting a camera trigger cable with the DSC connector be sure to insert it completely 22 Chapter 1 Assembly CONTACT ARM2 Connect to CONTACT ARM2 on control box B LAMP Connect to dia illumination lamphouse AC inlet Connect to AC power supply via power code REMOTE Connect to simple remote control pad HGFIE Connect to RS232C on motorized HG precentered fiber illuminator Ex OUT Connect to EXT I O on DS L3 U3 AC inlet Connect to AC power supply via power code i al CONTACT ARM1 Connect to CONTACT ARM1 on control box B DSC Connect to DSC Ni U on control box B or EXT I O on DS L3 U3 LAMP CTRL BOX B Connect to LAMP CTRL Ni U on control box
10. find any errors or wish to provide us with your opinion Introduction a Contents of the Manual The instruction manual for ECLIPSE Ni U is provided as two volumes 4 Operation 4 Assembly Maintenance This manual Safety Precautions Assembly Components Troubleshooting Microscopy Procedures Maintenance and Storage Operation Flowchart Specifications Bright Dark field Microscopy Epi fluorescence Microscopy Before reading the Assembly Maintenance manual Differential Interference Contrast Microscopy read the Safety Precautions in the Operation Phase Contrast Microscopy manual Motorized Bright field Microscopy Motorized Epi fluorescence Microscopy Individual Operations a Symbols Used in This Manual The following symbols are used in this manual 4 Symbols for Safety Z WARNING l l Highlights important information that should be noted for safety Read Safety Precautions for details AN CAUTION 4 Other Symbols Q Indicates information you should note or comply with to prevent defects or malfunction of this product Indicates information you should be aware of in using this product as well as other useful information Summary of Contents Summary of Contents See the next page for the detailed contents Introduction Assembly Contents of the Manual Symbols Used in This Manual Troubleshooting Maintenance and Storage Specifications 9beJo3s pue 2IULU
11. in the Assembly Maintenance Objectives cannot be switched smoothly Objectives are mounted on rather one side of the nosepiece Attach objective evenly The rotation stop function from address 1 to 6 7 is set Turn off rotation stop gt See Chapter 3 19 Operations on DS L3 19 1 Setting Up the Microscope 4 Configuring the settings related to the movement in the Operation The motorized nosepiece skips addresses The toggle function is ON Turn the toggle function OFF gt See Chapter 3 19 Operations on DS L3 19 1 Setting Up the Microscope 4 Configuring the settings related to the movement in the Operation 2 2 Epi fluorescence Microscopy Problem Cause Measure The mercury lamp does not work There is no power supplied Plug in the power cord Check your illuminator s manual The lamp has burned out Replace the lamp with the specified type Check your illuminator s manual The mercury lamp is not attached Attach a designated lamp Check your illuminator s manual The mercury lamp s connector is not connected to the illuminator Connect it to the power supply Check your illuminator s manual The mercury lamp burns out soon after it is turned on The lamp type is incorrect The lamp is at end of its life Replace the lamp with the specified type
12. 21 18 3 When attaching to the quadrocular tilting tube 21 18 4 When attaching a camera to the trinocular tube eee 21 19 Replace the ND filter Opti0Nal ooooonccnnicinnnnnconncconoccnccccnoncconrncanccnanccnnnnnnn 22 20 Connect the motorized device Cable oooooconicinicinccinnccnonccconnconnnnancnonnarancnnnnnno 22 21 Connect the power COM cococcocccnnccconcccocnconncnoncnnnn conc nnanccnnnn nan n carr anan carr nn ncnnncs 24 A Troll 1 Optical System and Operation cccccccceeeeeceeeeeeeceeeeeeeeeeeeeeeeeeeseaeeeeeeeeeeeseneeeess 1 1 Generalitat areas 1 2 Epi fluorescence Microscopy 1 3 Differential Interference Contrast Microscopy Contents 1 4 Phase Contrast MicroSCopy cooonccoconnccncnnoconcncnncnocono conc ancora nn cnn anorannccnnn 32 2 Electrical Requirement ooooooococcccccccccnncoooccnnnononannnnnononnncnnnnnnnnnnnnnnnnnnnnnnnnnncnnnnnanenes 33 2 1 Te alo Renee Perper eer aio 33 2 2 Epi fluorescence MiCrOSCOPY coccoccccoccconcconnnconnnnancnnancnnnnn non ncnnnc conc n ronca rancnnnncnns 34 Chapter e Maintenance and Storage cccoononcccccnncnnnnnonccnnnanononnnnnnnnrnnrnnanannnrrnnanannnos 35 1 Replacing the Lamp 0oooocccccccnccouococonocononanancncnnnnnonnnannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnns 36 2 A ee eee Se eee eer ee 37 2 1 Cleaning Lenses eiii isa 37 2 2 Cleaning Parts Other than the Lens eee cee eee ceeeeeee
13. Apo VC 60X H Plan Apo 60X Plan Apo 60X Fluor 60X W Apo TIRF 60X Oil Apo 60xW NIR Plan Fluor 60X Oil Plan Fluor 60X A Plan Apo A 60X Oil Apo 60X HAS Plan Apo VC 60XA WI eco Plan Apo IR 60X WI 100x Plan Apo VC 100X H Plan Apo A 100X Oil Apo TIRF 100X Oil Plan Apo 100X NCG Oil eco Plan Fluor 100X Oil Plan Fluor 100X Oil iris Plan 100X W N2 Dry 20x 40X N1 Dry 20X C 40X I C 40X Il 40X III 60X I 60X II 60X IV 100X 100X II 100X III NR Dry 60X I R 60X II R 60X IV R 100XI R 100X II R 16 Chapter 1 Assembly E Attaching the DIC module to DIC condenser oil D CUO is provided with two sliders Incorporate the oil condenser specific DIC modules D C DIC N2 OIL and D C DIC NR OIL to each slider Select the DIC module appropriate for the objective See the next table To support for particular purposes module for higher contrast or resolution are available Note however that in principle the contrast contradicts the resolution of the differential interference contrast image the higher the contrast the lower the resolution 1 Attach the DIC module to the slider Slider Identify the slider for the DIC module by the prism name sticker N2 and NR affixed to the handle and attach the DIC module to it Module orientation pin Align the module orientation groove on the
14. B CONTACT ARM1 Connect to CONTACT ARM1 on contact arm LAMP CTRL Ni U Connect to LAMP CTRL BOX B on Ni U main body USB connector Connect to USB H on DS L3 or PC DSC Ni U Connect to DSC on Ni U main body CONTACT ARM2 Connect to CONTACT ARM2 on contact arm 100 240V 1 0 50 60Hz DIA SHUTTER Connect to DIA motorized shutter EPI SHUTTER Connect to EPI motorized shutter Control box B rear CAMERA Connect to camera head USB H Connect to USB on control box B EXT I O Connect to DSC on Ni U main body or Ex OUT on control box B DS L3 DS camera control unit 23 Ajquiessy Ajquiessy Chapter 1 Assembly 21 Connect the power cord 1 Make sure that the microscope is turned off the power switch is pressed to 0 position 2 Plug the power cord into the AC inlet on the microscope main body 3 Plug the other end of each power cord into a wall outlet Q Connecting the power cord to the connected motorized device Check that the power switch for control box B or DS camera control unit is turned off plug the power cord into the AC inlet and then plug the other end to the wall outlet 24 Troubleshooting Misuse of this product may adversely affect performance even if this product is properly functional If any of the problems described in this chapter occurs be sure to check the table
15. Oil Apo 60X H AS Plan Apo VC 60XA WI eco Plan Apo IR 60X WI 100x Plan Apo VC 100X H Plan Apo A 100X Oil Apo TIRF 100X Oil Plan Apo 100X NCG Oil eco Plan Fluor 100X Oil Plan Fluor 100X Oil iris Plan 100X W N2 Oil 20x 40X 40X Il 40X III 60X I 60X II 60X IV 100XI 100X II 60X I R 60X II R NR Oil 60X IV R 100XI R 100X II R 100X III 18 Chapter 1 Assembly 14 Attach the nosepiece Tool Hex driver 2 mm across flats Lift the nosepiece at a position slightly toward yourself than directly below the arm and attach while sliding it back Continue sliding the nosepiece back until its front position is aligned with the front of the arm Tighten the fixing screw on the right side of the arm Nosepiece fixing screw Fixing the nosepiece 15 Attach the objective Screw the objective into the nosepiece Screw straight and all the way in Objective handling precautions When handling the objective be careful not to touch the tip of the lens and contaminate it with fingerprints Objective attaching sequence Attach the objective so that the magnification increases when the nosepiece is rotated clockwise when viewed from the top Attach the DIC rotation polarizer unit required for differential interference contrast microscopy Tool Hex driver 2 mm across flats 1 Loosen the po
16. approved according to EU EN standard 3 conductor grounding 3 conductor grounding Type HO5VV F 3 m long maximum rated at 250 VAC minimum e When used inside Japan PSE approved detachable power cord set 3 conductor grounding 3 conductor grounding Type VCTF 3 x 0 75 mm 3m long maximum rated at 125 VAC minimum 41 suonesi oads suonesuoads Chapter 4 Specifications m Control Box B for Nikon Microscope Model NI CTLB Connected to Ni U main body and contact arm Communication control Ni U main body and contact arm Motorized epi fluorescence cube turret intelligent epi fluorescence cube turret Motorized nosepiece intelligent nosepiece Motorized shutter up to two units Motorized HG precentered fiber illuminator Simple remote control pad DS L3 DS camera control unit PC Input ratings 100 240VAC 50 60Hz 1 0A Power cord e When used in 100 120 V regions outside Japan UL listed detachable power cord set 3 conductor grounding 3 conductor grounding Type SVT No 18 AWG 3 m long maximum rated at 125 VAC minimum e When used in 220 240 V regions Detachable power cord set approved according to EU EN standard 3 conductor grounding 3 conductor grounding Type HO5VV F 3 m long maximum rated at 250 VAC minimum e When used inside Japan PSE approved detachable power cord set 3 conductor grounding 3 conductor grounding Type VCTF 3 x 0 75 mm 3 m long maximum rated at 125 VAC min
17. i e becomes asymmetrically defocused when moving the focal point The nosepiece is not attached correctly or has not been fully rotated to the click stop position Attach it correctly and rotate it to the click stop position gt See Chapter 1 3 Assembly Method 14 Attach the nosepiece in the Assembly Maintenance The specimen is tilted relative to the stage surface Position the specimen in place on the stage See Chapter 2 2 Bright Dark field Microscopy 7 Place a specimen on the stage and move the stage to bring the target into view in the Operation The stage is tilted Attach the stage correctly gt See Chapter 1 3 Assembly Method 12 Attach the stage in the Assembly Maintenance The substage is tilted Attach the substage correctly gt See Chapter 1 3 Assembly Method 10 Attach the substage in the Assembly Maintenance The condenser is tilted Attach the condenser securely See Chapter 1 3 Assembly Method 13 Attach the condenser in the Assembly Maintenance Images in right and left eyepieces are not coincident Interpupillary adjustment has not been performed Perform interpupillary adjustment gt See Chapter 2 2 Bright Dark field Microscopy 10 Adjust the interpupillary distance in the Operation Diopter adjustment has not been performed Perform diopter adjustment
18. or grease Moisten lightly a piece of soft clean cotton cloth lens tissue or gauze with absolute alcohol ethyl or methyl alcohol and wipe the dirt off Tips on wiping Do not reuse cotton cloth lens tissue or gauze that have already been used 2 2 Cleaning Parts Other than the Lens Cleaning light dirt dust Wipe with a silicon cloth Cleaning tough dirt fingerprint or grease Dampen a piece of gauze a little with neutral detergent and wipe the dirt gently 37 abe10j s pue a9ueuajuIelA abei0js pue sdueusuleW Chapter 3 Maintenance and Storage 2 3 Cleaning Immersion Oil 1 Wipe with petroleum benzine 2 Finish off the cleaning with absolute alcohol ethyl or methyl alcohol after cleaning with petroleum benzene If petroleum benzene is not available If petroleum benzene is unavailable use methyl alcohol alone However typically wipe three or four times because the detergency is weak 2 4 Decontaminating the Product For routine disinfection of this product Nikon recommends using 70 medical alcohol Use of organic solvents on plastic parts may result in discoloration Cautions on disposal If contact occurs between a sample and this product determine whether the sample is hazardous If the sample is hazardous follow the standard procedures for your facility 3 Transportation Before transporting the following devices be sure to fasten the devices e Manual quadrocular tilti
19. significantly To support for particular purposes module for higher contrast or resolution are available Note however that in principle the contrast contradicts the resolution of the differential interference contrast image the higher the contrast the lower the resolution e PH module required for phase contrast microscopy There are PH modules D C PH 1 D C PH 2 and D C PH 3 Select the PH module with a matching PH code with the PH objective e Dark field module required for dark field microscopy The dark field module D C DF can be used with any objective with a numerical aperture NA of 0 7 or less Note however that 2x and 4x objectives are not supported e 2 4x auxiliary lens required for low magnification bright field microscopy 2x and 4x objectives can only be used for bright field microscopy In this case select a 2 4x auxiliary lens Two types of 2 4x auxiliary lens are available with different diameter and different condenser turret attachment address 2 4x auxiliary lens NI CAL N1 and 2 4x auxiliary lens D C 2 4x 13 Ajquiessy Ajquiessy Chapter 1 Assembly NI CUD Universal Condenser Structure Replacement cover fixing screw two Replacement cover removed Module replacement cover number 5 la E urret lens part PH module centering knob Use the centering knob by pulling it out Condenser y R horizontally when centering Otherwise ICT tt A aperture a keep it folded
20. universal condenser s turret is at the intermediate position Rotate it to the click stop position gt See Chapter 3 7 2 Using the NI CUD Universal Condenser Dry in the Operation The DIC slider is at the intermediate position for an oil condenser Push in the slider until it reaches the limit See Chapter 3 7 3 Using the D CUO DIC Condenser Oil in the Operation The DIC module on the objective is at the intermediate position Attach it correctly gt See Chapter 3 15 2 Using Optical Elements in the Operation Incomplete attachment of nosepiece Attach it correctly See Chapter 1 3 Assembly Method 14 Attach the nosepiece in the Assembly Maintenance The polarizer and an analyzer are at the intermediate position Switch over correctly gt See Chapter 3 15 2 Using Optical Elements in the Operation The lambda plate is at the intermediate position Make sure that it is attached to the limit See Chapter 3 15 2 Using Optical Elements in the Operation No contrast The polarizer is out of the optical path Bring the polarizer into the optical path gt See Chapter 2 4 Differential Interference Contrast Microscopy 14 Adjust the orientation vibration direction of the polarizer and analyzer in the Operation The analyzer is out of the optical path Bring the analyzer i
21. Check your illuminator s manual m Motorized epi fluorescence cube turret Problem Cause Measure The filter cube does not switch The motorized epi fluorescence cube turret is attached incorrectly Attach it correctly gt See Chapter 1 3 Assembly Method 6 Attach the epi fluorescence cube turret and epi fluorescence attachment W Attaching a filter cube in the Assembly Maintenance In layered configuration motorized or intelligent epi fluorescence cube turret is connected to the 2nd layer Replace the motorized or intelligent epi fluorescence cube turret in the 2nd layer with the manual epi fluorescence cube turret The shutter does not move The motorized epi fluorescence cube turret is attached incorrectly Attach it correctly gt See Chapter 1 3 Assembly Method 6 Attach the epi fluorescence cube turret and epi fluorescence attachment M Attaching a filter cube in the Assembly Maintenance Cannot configure to OPEN Configuration OPEN is exclusive to epi fluorescence cube turret address 1 Other addresses cannot be configured to OPEN Use it as it is 34 Chapter Maintenance and Storage This chapter describes how to replace the halogen lamp and how to clean and store the product 35 abe10 S pue soueuajuley abei0js pue 9JUBUAJUIBIA Chapter 3 Maintenance and Storage 1 Replacing the Lamp N CAUT
22. Condenser in the Operation 5 An aperture diaphragm is stopped down 5 Open it to proper size too far gt See Chapter 3 6 Adjusting the Aperture Diaphragm in the Operation Dirt or dust on the monitor moves when the camera is turned gt Lens or specimen is dirty or dusty Check and clean it in accordance with the Dirt or dust does not rotate when eyepiece is turned of Dirty or Dirt or dust appears on the dusty field of view when looking into eyepiece monitor Dirt or dust on the monitor does not move when the camera is turned The camera is dirty Remove the camera and clean it in accordance with the camera s instruction manual No cover glass is attached Attach a cover glass of the specified thickness 0 17 mm However no cover glass is required for an The thickness of the cover glass is inadequate NCG objective A high magnification objective appropriate for Use the high magnification objective e g CFI LWD hemocytometer cover glass thickness 0 4 mm 40xC appropriate for observation using thick cover Image quality is poor 0 7 mm is not used glass Contrast is poor Resolution The objective correction ring does not match the is poor thickness of the cover glass for the objective Correct the ring as appropriate with a correction ring Check and clean them in accordance with Dirt or dust does not rotate when eyepiece is turned of Dirty or dusty
23. IJUIEIA Bunooys jqnos quiassy suonyesoads Contents Introduction Contents of the Manual c22 2 lt ccciseescxeccencdees sse0cces saves 420 ocacaceddnccacescdncedwesdaacacdesdescdadectseasteans ii Symbols Used in This Manual Summary of Content 2 cccccceccceeeeceeeeeceeeeseaeeceeenaeeeeececeeescaeeseeseeeeeseeeeeseneeeeeeaees iii Chapter 1 Ec A 1 ECLIPSE Ni U System Configuration oooooconcococonocccocononcccconnncnnnnnnncnnonnnncnnnnnnnnnnnnnnn 2 2 Components List ooooooocccconoccccnocococcnonocnnonnnncononnncnnnnnnnnnnnnnn nn nn nnnnnnnnnnnnnnnnnnnnnnnnnnnninines 3 3 Assembly MEOtNOd c ccoo aliaron iia iaee 4 Into dUCt ON as 4 1 Check the input VO tage oocoooocccnnccnnnccconacooccnonccnnnccnanccancnnancncnnn nan nnan nn nannannnncnnns 4 2 Attach the DIA motorized shutter optional ooooooncccnnndinncnnnnccnccnncccnrncancnnannnono 5 3 Attach the Ham corona 5 4 Attach the lamphouse for dia illUMINAtION eee eeee eee eeeeeteeeeeteeteeeteeeteeeees 5 5 Attach the standard arm contact arm ccccccceseeeeeeeeeeeeeeeaeeseeeeeaeeseeeseaeeteeeees 6 6 Attach the epi fluorescence cube turret and epi fluorescence attachment required for epi fluorescence MICrOSCOPY ceeceeeeeeeeeeeteeeeeeeeeeteeeeneeesaeenees 7 m Attaching a filter cube oo ce eee tee eee teaetaeeeaeteeetaeeeeeseeeeaeeeaeeaes 8 m Replacing excitation and barrier filters
24. ION e Beware of burns Wait until the lamp and nearby parts have cooled approximately 30 minutes before replacing the lamp e Beware of electrical shock Turn off the power switch and unplug the power cord from the wall outlet e Beware of abnormal heat generation Use only the designated lamp e Beware of soiling Avoid touching the glass surface of the lamp with bare hands Soiling may reduce the service life of the lamp e Lamphouse cover Make sure the lamphouse cover is securely fitted to the lamphouse after lamp replacement e Used lamps Do not break used lamps It should be disposed of as industrial waste according to local regulations and rules Tool Hex wrench 3 mm across flats 1 Loosen the lamphouse cover fixing screw and lift up the cover insertthe hexwrench a d to remove loosen the fixing screw 2 Hold down the lamp clamp lever and remove the old lamp 3 Attach a new lamp Avoid touching the glass surface of the Fixing screw lamp with your bare hands Attach the new lamp while holding down the lamp clamp lever Put the lamp clamp lever back to its original position Designated lamp PHILIPS7724 or OSRAM HLX64623 4 Reattach the cover back to its original position and tighten the Lamphouse cover lamphouse cover fixing screw Removing the lamphouse cover Lamp Lamphouse fixing screw Lamp clamp lever Attaching the lamp 36 Chapter 3 Maintenance and Storage 2 Clean a
25. Insert the lamp clamp lever to open the socket pin hole Attach the lamp while holding down the lever Put the lamp clamp lever back to its original position Designated lamp PHILIPS7724 or OSRAM HLX64623 Reattach the cover back to its original position and tighten the lamphouse cover fixing screw 4 Attach the lamphouse for dia illumination Tool Hex driver 2 mm across flats 1 2 Insert the lamphouse at the rear of the microscope and tighten the fixing screws Connect the dia illumination lamphouse cable to the LAMP connector on the rear of the main body Insert the hex wrench and loosen the fixing screw Fixing screw Removing the lamphouse cover Lamp Lamphouse fixing screw Lamp clamp lever Attaching the lamp Lamphouse fixing screw Securing the dia illumination lamphouse Ajquiessy Ajquiessy Chapter 1 Assembly 5 Attach the standard arm contact arm The attachment procedure is the same for standard arm and contact arm To use the motorized accessories attach the contact arm The contact arm has a contact When attaching a motorized device on the arm remove the contact cover beforehand Tool Hex wrench 4 mm across flats 1 Place the arm while aligning it with the two positioning pins on the main body and tighten the four fixing screws 2 When using contact arm connect the cable from the CONTACT ARM1 connector to the CONTACT ARM1 connector on the control bo
26. Tighten the fixing screw loosened in step 3 6 Tighten the four fixing screws on top of the epi fluorescence attachment 7 Attach the HG adapter to the bayonet mount at the rear of the epi fluorescence attachment and connect the HG precentered fiber illuminator See the instruction manual provided with the HG precentered fiber illuminator Epi fluorescence cube turret Positioning hole Epi fluorescence attachment Connection fixing screw Connecting epi fluorescence cube turret and epi fluorescence attachment I Epi fluorescence attachment Laa fixing screw Fixing screw Attaching and fixing epi fluorescence cube turret and epi fluorescence attachment set Bayonet mount HG adapter HG fiber Attaching the HG adapter Ajquiaessy Ajquiessy Chapter 1 Assembly E Attaching a filter cube AN Precautions for attaching and removing the cube e Be sure to check that the light source is turned off before attaching or removing the cube e Be sure to check that the power switch for microscope main body is turned off and then attach the cube by rotating the cube switchover turret Also for the motorized epi fluorescence cube turret attach it by manually rotating the inside turret 1 Pull out the filter cube replacement cover on the front of the epi fluorescence cube turret to remove it 2 Attach the filter cube to the slot 3 Insert the filter cube nameplate into the
27. ached or removed 10 Chapter 1 Assembly 9 Attach eyepieces Make sure the notch on the eyepiece side and the protrusion of the eyepiece sleeve are aligned then insert the eyepieces Notch on eyepiece The eyepiece has a notch to prevent rotation When attaching match the notch with the protrusion on the eyepiece sleeve The eyepiece lens will not be positioned properly if the notch is not matched with _ the protrusion 10 Attach the substage Attach NI SS substage or NI SSR substage for rotatable stage suitable for the type of the stage used The procedure is the same for both models Tools Hex driver 2 mm across flats Hex wrench 3 mm across flats 1 Place the square groove on the substage onto the reference guide bar on the elevating section of the main body Move the substage to the left press it against the reference surface of the elevating section and tighten the drop proof fixing screws x3 with hex wrench 3 mm across flats 2 Put the provided elevating section cover onto a front of the elevating section and tighten the provided two screws with hex driver 2 mm across flats to secure 11 Notch Protrusion Attaching eyepieces Substage fixing screw Reference guide bar Square groove Substage fixing screw Substage from the front Attaching the elevating section cover iquiassy Chapter 1 Assembly Ajquiessy
28. adrocular tilting tube See Chapter 1 3 Assembly Method 7 Attach the quadrocular tilting tube and DSC zooming port for quadrocular tube in the Assembly Maintenance 29 Bulooysa qno Bunooys jqnoIL Chapter 2 Troubleshooting 1 2 Epi fluorescence Microscopy Problem Cause Measure Lack of visibility around periphery of field of view Illumination is uneven across the field of view Field of view is not visible The filter cube is misaligned Push the cube in to the limit gt See Chapter 1 3 Assembly Method 6 Attach the epi fluorescence cube turret and epi fluorescence attachment W Attaching a filter cube in the Assembly Maintenance A fluorescent image is not visible when the lamp is ON The shutter is closed Open the shutter See Chapter 3 14 3 Protecting the Sample and Preventing It from Decoloration Using the Shutter in the Operation The selection of the filter cube is incorrect Use a correct filter cube gt See Chapter 3 14 2 Selecting Filters in the Operation The fluorescent image is very dark when the lamp is ON The ND filters of the epi fluorescence attachment are in the optical path Remove the ND filters from the optical path as necessary See Chapter 3 14 4 Adjusting the Brightness of the Fluorescent Image Using ND Filters and the Aperture Diaphragm in the Operatio
29. aeteeeteeeeeeeaees 37 2 3 Cleaning Immersion Olleria 38 2 4 Decontaminating the Product eee eee certs eee eeeseeetaeeeeeeeeteeeeeeaees 38 3 TRANS POMation ccoo ici naaa aaa ai aa a S AEA ERa 38 SOAGE oiccen eiee eere eE ee E eaae e e aap oiea Ea ER eaea 38 Regular Inspections Charged c ccccesecceeeeeeeeeceeeeeeeeeeeeeeeseeaeeeeeeeaeeeeseneeeees 38 Chapter 4 SSO CIPLC AR INS ef cictecatoss etch fae acest cone sindees ceccseciss cneeansetneonetentececke idaaeas 39 1 Microscopy Principles cccccccceeeeeceeeeeeceeeeeeeeeeeeeeeeeeeeeeeeeeeeaeeeeeseeseeessseneeees 40 Performance Properties cccccccceeeeeeceeeeeceeeeeeeeeeeeeeneeeeecaeeeeeeeaeeeeeeneaeeeeeenneeees 40 Physical Properties miccional ia ced compels 43 Contents vi Assembly This chapter contains an Ni U system configuration diagram a list of its components and explains how to assemble the system Read appropriate notes such as CAUTION 9 Cautions on assembling and installing the product at the beginning of the separately provided instruction manual Operation or 4 Installation location in Notes on Handling the Product in the same manual before you work on assembling m Assembly tools provided with the microscope e Hex driver x3 2 mm across flats x3 e Hex wrench x2 3 and 4 mm across flats Ajquassy Ajquiessy Chapter 1 Assembly ECLIPSE Ni U System Configuration ENG mount camera C
30. apter 2 Troubleshooting Problem Cause Measure Bunooys jqnoIL Adjust the orientation of the optical system correctly gt See Chapter 2 4 Differential Interference Contrast The orientation of the polarizer is incorrect Microscopy 14 Adjust the orientation vibration direction of the polarizer and analyzer in the Operation Select the DIC module suitable for the objective used A correct DIC module on the condenser has not See Chapter 1 3 Assembly Method 13 Attach the been selected condenser W Attaching the optical module to the universal condenser and W Attaching the DIC module to DIC condenser oil in the Assembly Maintenance Use the DIC slider suitable for the objective gt See Chapter 1 3 Assembly Method 13 Attach the condenser M Attaching the optical module to the universal condenser and W Attaching the DIC module to DIC condenser oil in the Assembly Maintenance Incorrect combination of the objective and the Poor contrast DIC slider on the objective Gently wipe dust off Special attention should be a paid to dust Dusty objective condenser and specimen See Chapter 3 2 1 Cleaning Lenses in the Assembly Maintenance Make sure the condenser is focused and centered The field diaphragm image is not focused on the specimen surface gt See Chapter 3 5 Focusing and Centering the Condenser
31. ating section of the main body Stage e Right left handle stage with specimen holder e Right left handle ceramic coated stage with specimen holder Right handle ceramic coated stage without specimen holder as well e Right left handle rotatable ceramic coated stage with specimen holder Travel range X 78 mm Y 54 mm Rotation angle The stage can be rotated clockwise by 195 and counterclockwise by 7 from the reference position rotation angle 0 Condenser holder Equipped with condenser focus knob condenser centering screws Attached to a substage Condenser Universal condenser Attach optical modules for differential interference contrast phase contrast or dark field microscopy to a turret C C phase turret condenser Optical module for phase contrast and dark field and shutter embedded DIC condenser oil Differential interference contrast optical module OIL slider attached Other condensers Abbe condenser achromat condenser dark field condenser dry and oil aplanatic condenser X LWD condenser 2 100X slide achro condenser 1 100X achromat swing out condenser Ni U microscope input ratings 100 240VAC 50 60Hz 1 7A Power cord e When used in 100 120 V regions outside Japan UL listed detachable power cord set 3 conductor grounding 3 conductor grounding Type SVT No 18 AWG 3 m long maximum rated at 125 VAC minimum e When used in 220 240 V regions Detachable power cord set
32. away 3 Attach the nameplate sticker Affix a nameplate sticker for each module to the turret perimeter Arrange stickers so that when the condenser is attached to the microscope the module name currently in the optical path is always indicated by the sticker at the front position i e stickers are put across from the corresponding module Put the sticker O for the empty hole Put the sticker NR provided with the DIC module when NR type of DIC module on the condenser has been attached This sticker is not provided with the universal condenser 15 Ajquiessy Ajquiessy Chapter 1 Assembly Combination of DIC slider on the objective and DIC module on the condenser when using the NI CUD universal condenser Objective Standard combination Contrast oriented Resolution oriented DIC module on the condenser DIC slider on the objective DIC module on the condenser DIC slider on the objective DIC module on the condenser DIC slider on the objective 10x Plan Fluor 10X Plan Apo 10XA DIC allowed for eco only Plan Apo 10X S Fluor 10X Plan Fluor 10X W N1 Dry 10x 20x Plan Fluor 20X Plan Fluor 20X MI Plan Apo 20X Plan Apo VC 20X Plan Apo 20X S Fluor 20X Fluor 20X W 40x Plan Fluor 40X Plan Apo 40X Plan Apo 40X S Fluor 40X Apo LWD 40X WI 1S Plan Fluor 40X Oil S Fluor 40X Oil Apo 40X WI AS Fluor 40X W Apo 40X W NIR 60x Plan
33. bottom of the DIC module with the slider s orientation pin and tighten the DIC module fixing screw on the slider DIC module Module orientation groove DIC module mounted DIC module fixing screw Attaching the DIC module 2 Insert the DIC module slider into the condenser For the differential interference contrast microscopy insert the slider with the DIC module until it reaches the condenser main body Pull out the slider from the condenser when switching over to the bright field microscopy 17 Ajquiessy Ajquiessy Chapter 1 Assembly Combination of DIC slider on the objective and DIC module on the condenser when using the D CUO DIC condenser Objective Standard combination Resolution oriented DIC module on the condenser DIC slider on the objective DIC module DIC slider on the condenser on the objective 10x Plan Fluor 10X Plan Apo 10XA allowed for eco only Plan Apo 10X S Fluor 10X Plan Fluor 10X W 20x Plan Fluor 20X Plan Fluor 20X MI Plan Apo 20X Plan Apo VC 20X Plan Apo 20X S Fluor 20X Fluor 20X W 40x Plan Fluor 40X Plan Apo 40X Plan Apo 40X S Fluor 40X Apo LWD 40X WI 1S Plan Fluor 40X Oil S Fluor 40X Oil Apo 40X WI AS Fluor 40X W Apo 40X W NIR 60x Plan Apo VC 60X H Plan Apo 60X Plan Apo 60X Fluor 60X W Apo TIRF 60X Oil Apo 60xW NIR Plan Fluor 60X Oil Plan Fluor 60X A Plan Apo A 60X
34. camera TV adapter TV adapter Y TV ENG mount camera or photomicroscopy device with an adapter Y TV55 Y TV55 can be attached to C TF or C TT trinocular tube Connect a C mount camera with C mount adapter 0 55x Ajquiessy Ajquiessy Chapter 1 Assembly 3 Assembly Method Introduction Ni U is a manual microscope Basically a manual model is attached for each device consisting the microscope However for nosepiece epi fluorescence cube turret and HG precentered fiber illuminator motorized models can be attached In this case contact arm control box B and simple remote control pad are required In addition in order to configure information for motorized devices DS L3 DS camera control unit must be connected Motorized devices on Ni U can be controlled from DS L3 Note that when using epi fluorescence cube turrets by layering motorized intelligent epi fluorescence cube turret can only be used in 1st layer Assemble the device according to the following procedure Precautions for connecting cables Cable connections are required for some motorized devices Be sure to turn off the power to the microscope and peripheral devices before starting cable connection Cable connections are described in each assembly step Nikon recommends connecting cables at the end of the assembly See 20 Connect the motorized device cable for the connector connections Information setting regarding motorized mod
35. down diaphragm lever Round dovetail lock pin 1 Remove the cover Remove two screws securing the module replacement cover using the provided hex driver and remove the cover 2 Attach the optical module A number is shown near the mounting hole Attach the optical module in the specified hole Turn the turret to bring the desired hole to an accessible position for attaching the optical module Place the module with its name facing up Module positioning hole number PH module Hole Nameplate sticker Module oes PH module Empty For bright field centering screw 1 microscopy module not attachable N Turret UN 2 PH module D C PH 1 PH module or D C PH3 3 PH module D C PH 2 is attached 4 PH module D C PH 3 DIC module D C DIC N2 DRY 5 DIC module Sr D C DIC NR DRY Hole number 1 to 7 6 Dark field module D C DF 2 4x auxiliary lens Positioning pin D C 2 4x 39 DIC module Put nameplate sticker DIC module ey p PeDe NIOR D C DIC N2 DRY is Ph3 attached a 7 Module fixing screw 2 4x auxiliary lens NI CALN1 935 Positioning DIC module on groove s the condenser 14 Chapter 1 Assembly Procedure for attaching the DIC module on the condenser dark field module and 2 4x auxiliary lens Holes 5 6 and 7 have their
36. e field diaphragm slightly wider than the field of view gt See Chapter 3 8 Adjusting the Field Diaphragm in the Operation Lack of visibility around periphery of field of view Illumination is uneven across the field of view Field of view is not visible Incorrect combination of the objective with the condenser Adopt an appropriate combination gt See Chapter 3 7 1 Compatibility of Condenser Type and Objective Magnification in the Operation When using objectives with 4x or less 2 4x auxiliary lens of universal condenser is not in the optical path or condenser switching such as swing out or sliding operation is not performed Bring 2 4x auxiliary lens of universal condenser in optical path For other condensers swing out or slide the condenser The lamp is attached incorrectly Attach it correctly See Chapter 1 3 Assembly Method 3 Attach the lamp in the Assembly Maintenance The lens and specimen are dirty or dusty Clean them as appropriate gt See Chapter 3 2 1 Cleaning Lenses in the Assembly Maintenance Out of focus with an objective of high magnification The specimen is upside down Turn up the cover glass and attach it to the stage See Chapter 2 2 Bright Dark field Microscopy 7 Place a specimen on the stage and move the stage to bring the target into view in the Operation The thickness of
37. els Set information of the motorized devices or optical element attached on the microscope setup menu for the DS L3 DS Camera L Control Unit See Chapter 3 19 Operations on DS L3 19 1 Setting Up the Microscope Ed Check the input voltage Check the input voltage indicated on the back of the microscope Use the microscope only if the indicated input voltage matches the power supply voltage for the area in which the microscope will be used Tn A Input voltage Z WARNING label If the indicated voltage and the supplied voltage differ do not attempt to use the microscope Contact your nearest Nikon representative Checking the input voltage E Microscope rear view Unlocking the elevating section Tool flathead screwdriver After you confirm that the input voltage is correct loosen and remove the tightening screw at the front of the elevating section f Tightening screw Unlocking the elevating section Chapter 1 Assembly 2 Attach the DIA motorized shutter optional The motorized shutter is attached by Nikon Contact your nearest Nikon representative when the DIA motorized shutter needs to be attached or removed 3 Attach the lamp Tool Hex wrench 3 mm across flats 1 2 8 Lamp handling precautions Avoid touching the glass surface of the lamp with your bare hands Loosen the lamphouse cover fixing screw and lift up the cover to remove
38. field of view when looking into eyepiece The lens and specimen are dirty or dusty 26 Chapter 2 Troubleshooting Problem Cause Measure Image quality is poor Contrast is poor Resolution is poor An aperture diaphragm is stopped down too far Otherwise it is open too much Open it to the proper size See Chapter 3 6 Adjusting the Aperture Diaphragm in the Operation The field diaphragm image is not focused on the specimen surface Condenser adjustment is incorrect Make sure the condenser is focused and centered See Chapter 3 5 Focusing and Centering the Condenser in the Operation No immersion oil is applied to the tip of an oil immersion objective The designated immersion oil is not used Apply our designated non fluorescent immersion oil See Chapter 3 12 Oil Immersion Water Immersion in the Operation The immersion oil contains air bubbles Remove the air bubbles gt See Chapter 3 12 Oil Immersion Water Immersion in the Operation The immersion oil adheres to the tip of the dry type objective Clean it as appropriate gt See Chapter 3 12 Oil Immersion Water Immersion in the Operation Field of view is too bright The ND filters are out of the optical path Place the ND filters into the optical path See Chapter 3 1 2 Adjustment with ND Filters in t
39. for possible causes before requesting service If you detect problems that are not listed in the table or the problem still persists after measures are taken turn off the device and contact your nearest Nikon representative 25 Buljooysajqnoiy Chapter 2 Troubleshooting 1 Optical System and Operation Problem Cause Measure Bunooys jqnoIL Dirt or dust rotates when the eyepiece is turned The eyepiece is dirty Clean the eyepiece See Chapter 3 2 1 Cleaning Lenses in the Assembly Maintenance Dirt or dust does not rotate when eyepiece is turned P 1 to 5 1 The specimen is dirty if dirt or dust moves 1 Clean the specimen when the specimen is moved on stage 2 The tip of the condenser lens is dirty if dirt 2 Clean the condenser or dust goes in and out of view when the See Chapter 3 2 1 Cleaning Lenses in the condenser is moved up and down while Assembly Maintenance using a low magnification objective Dirty or dusty field of view when looking into eyepiece 3 The objective is dirty if dirt or dust 3 Clean the objective disappears when the objective is switched gt See Chapter 3 2 1 Cleaning Lenses in the Assembly Maintenance 4 The field diaphragm image is not focused 4 Make sure the condenser is focused and on the specimen surface Condenser centered adjustment is incorrect See Chapter 3 5 Focusing and Centering the
40. he Operation The lamp voltage is too high Turn the brightness control knob to the Xd mark position and adjust the brightness with the ND filters See Chapter 3 1 Adjusting the Brightness of a Diascopic Image in the Operation Field of view is too dark The lamp voltage is too low Turn the brightness control knob to the Xd mark position and adjust the brightness with the ND filters See Chapter 3 1 Adjusting the Brightness of a Diascopic Image in the Operation The condenser aperture diaphragm is stopped down too far This should normally be adjusted to 70 to 80 of numerical aperture of the objective gt See Chapter 3 6 Adjusting the Aperture Diaphragm in the Operation The field diaphragm image is not focused on the specimen surface Make sure the condenser is focused and centered See Chapter 3 5 Focusing and Centering the Condenser in the Operation The optical path is not switched to binocular 100 Set to binocular 100 gt See Chapter 3 Section 10 Switching the Optical Path of the Tube in the Operation Image is yellowish or very bluish The NCB11 filter is not used Use the NCB11 filter See Chapter 3 1 2 Adjustment with ND Filters in the Operation Lamp voltage is too low or too high Turn the brightness control knob to the re mark position and adjust the brightness
41. ical care This microscope is intended for use in microscopic examination diagnostics observation and experiment of cells and tissues at hospitals or other facilities or by doctors in private practice in the field of pathology anatomy and cytology The microscopy with diascopic reflected illuminations is used to observe a sample fixed on the slide cells and tissue as the specimen The product is classified as an in vitro diagnostic medical device This product is not intended for use for measurement The scale on the focus knob and stage is an indicator to reproduce the position and does not guarantee the value of the thickness or length of a sample measured using this scale a Intended user It is intended for the researchers medical professional and those who work on experiments in the field of pathology and cytology 2 Performance Properties m Nikon Microscope ECLIPSE Ni U Model ECLIPSE Ni U Main body Optical system Infinity corrected CF optical system Objective CFI60 Eyepiece Field number 22 when using ergonomic tube or binocular tube Field number 25 when using trinocular tube T F or quadrocular tilting tube O Field number when used in two layers When two epi fluorescence cube turrets are used by layering field number is 22 Focusing unit Vertical movement Manual one axis coarse fine adjustment knob motion calibration markings for fine motion 1 um per scale Stroke 3 mm upward 26 mm downward focal pos
42. ight shielding plate Tool Hex driver 2 mm across flats Lightly screw the two fixing screws provided with the light shielding plate into the front bottom of the epi fluorescence cube turret Hook the convex part of the light shielding plate to the two fixing screws and tighten the fixing screws Turn approx 30 degrees to secure in place Barrier filter Excitation filter Replacing the excitation and barrier filters Fixing screw At the bottom of the epi fluorescence cube turret Fixing the light shielding plate Ajquessy Chapter 1 Assembly Ajquiessy Attach the quadrocular tilting tube and DSC zooming port for quadrocular tube Tools Hex driver 2 mm across flats Hex driver 2 5 mm across flats provided with DSC zooming port Hex wrench 3 mm across flats Hex wrench 4 mm across flats First connect the quadrocular tilting tube and DSC zooming port for quadrocular tube then attach them on the microscope arm if epi fluorescence cube turret or epi fluorescence attachment is attached on top of that 1 Remove the tightening screws at the bottom of the tube with the hex wrench 4 mm across flats Tighis g screw 2 Insert the positioning pin of the DSC zooming port while aligning it with the positioning hole on the tube side and then tighten the connection fixing screws 2 5 mm across flats on the top of the tube with a hex wrench 3 Loosen the fixing screw o
43. ilter optional Tool Hex driver 2 mm across flats The ND filter in the ND filter cassette of the Ni U main body can be replaced as follows 1 Check that the microscope power switch is OFF 2 Press all ND filter IN OUT switches to the IN side 3 Remove the rubber cap attached to the top of the 4 Insert a hex driver in the hole with the rubber cap filter cassette cover it can be removed with your 2 finger sy Press all switches removed and loosen the internal screw The screw to the IN side is drop proof and not detached from the cover Ram ving the filter cas ati 5 Hold the ND filter IN OUT switch and pull out the 6 Hold the end of the filter holding frame and widen 7 When attaching the filter place the filter on the cassette slightly to remove the filter holding frame at the position indicated with the arrow and slide it in Replacing the filter Connect the motorized device cable O Precautions for connecting cables Make sure that the microscope and peripheral equipment are turned off before connecting the cable When the microscope is motorized see the figure on next page and connect the cable correctly To connect the LAMP CTRL BOX B on the microscope to the LAMP CTRL Ni U on the control box B the lamp control cable is required To connect the DSC on the microscope to the DSC Ni U on the control box B the C CTC camera trigger cable
44. imum Simple Remote Control Pad for Nikon Microscope Model NI SRCP Connected to Control box B Control Motorized epi fluorescence cube turret Motorized nosepiece Motorized shutter up to two shutters Motorized HG precentered fiber illuminator s ND value shutter Switch indicator Eight function switches One operation toggle switch 10 LED indicators 42 Chapter 4 Specifications 3 Physical Properties m Nikon Microscope ECLIPSE Ni U Model ECLIPSE Ni U Operating conditions Temperature 0 C to 40 C Humidity 60 RH max no condensation Altitude 2000 m max Pollution degree Degree 2 Installation Category II Electrical shock protection class Class Indoor use only Transport storage conditions Temperature 20 C to 60 C Humidity 90 RH max no condensation External dimensions and Ni U main body weight Dimensions 320 W x 315 H x 383 D mm excluding projections Weight Approx 11 kg Safety standards e UL cUL recognized product Including the following motorized devices e NI CTLB Control Box B e NIU CAM Contact Arm e NI LH Precentered Lamphouse e NI SRCP Simple Remote Control Pad e NI N7 E Motorized Septuple Nosepiece e NI ND6 E Motorized DIC Sextuple Nosepiece e NI N7 I Intelligent Septuple Nosepiece e NI ND6 I Intelligent DIC Sextuple Nosepiece e NI FLT6 E Motorized Epi fluorescence Cube Turret e NI FLT6 I Intelligent Epi fluoresce
45. in the Operation The immersion oil is not sufficient in the oil Apply the immersion oil again condenser and air bubbles are between the gt See Chapter 3 12 Oil Immersion Water Immersion in slide and the condenser the Operation 1 4 Phase Contrast Microscopy Problem Cause Measure Adjust so that they match The PH module of the condenser does not match the phase plate image of the objective See Chapter 2 5 Phase Contrast Microscopy 14 Center the PH module in the Operation Put the PH module with the same Ph code as the objective into the optical path The PH module of the condenser does not match the Ph code of the objective See Chapter 2 5 Phase Contrast Microscopy 16 Adjust the PH module in the condenser with the Ph Poor contrast objective to be used in the Operation Change the mounting agent or thickness of the specimen when preparing the specimen The phase contrast of the specimen is too large See Chapter 3 16 1 Tips for Phase Contrast Microscopy in the Operation ae Use a Ph objective suitable for the specimen The type of the Ph objective is not suitable for phase contrast of the specimen gt See Chapter 3 16 1 Tips for Phase Contrast Microscopy in the Operation 32 Chapter 2 Troubleshooting 2 Electrical Requirements 2 Sener m Power Problem Cause Measure
46. ition as a reference Coarse focus knob Approx 7 8 mm rotation Fine focus knob Approx 0 1 mm rotation With refocusing mechanism Dia illumination Fly eye illumination 12V 100W illumination power supply integrated 100 240V with system photomicrography voltage function Light source 12V100W long life halogen lamp Designated lamphouse NI LH precentered lamphouse Designated lamp PHILIPS 7724 or OSRAM HLX64623 ND filter cassette 4 holders integrated NCB11 ND8 ND32 and empty Filter removable replaceable Diffuser integrated non removable 40 Chapter 4 Specifications Tube Binocular tube binocular section 100 Trinocular tube F Binocular adapter 100 0 0 100 Trinocular tube T Binocular adapter 100 0 20 80 0 100 Ergonomic tube Binocular section 100 with DSC port mounted binocular DSC port 50 50 Quadrocular tilting tube Binocular adapter 100 0 0 100 with DSC port mounted binocular adapter DSC port 0 0 100 Arm Standard arm Contact arm Required for motorized operation Nosepiece Universal quintuple BD quintuple sextuple sextuple with analyzer slot DIC sextuple intelligent DIC sextuple septuple intelligent motorized DIC sextuple and motorized septuple Substage Two types for standard stage and rotatable ceramic coated stage one for rotatable ceramic coated stage is equipped with rotatable stage centering screws Attached to the elev
47. larizer rotation clamp screw rotate the Polarizer rotation clamp screw polarizer to align the indicator lines and then clamp it 2 Put the rotatable polarizer unit over the field lens on the microscope base 3 Use the hex driver to tighten the polarizer unit fixing screw at the position where the indicator line faces the front Be sure to adjust the vibration direction before the microscopy Y Polarizer unit See Chapter 2 4 Differential Interference Indicator fixing screw Contrast Microscopy 14 Adjust the orientation vibration direction of the polarizer and analyzer Fixing the polarizer in Operation instruction manual xing polari 19 Ajquessy Ajquiessy Chapter 1 Assembly 17 Attach the eyelevel riser optional Tool Hex driver 2 mm across flats Place the eyelevel riser on the arm and tighten the fixing screws on the front of the arm using a hex driver 2 mm across flats Tool Hex driver 2 mm across flats 1 Screw the camera head into the C mount on the DSC port 2 Remove the rear cover of the ergonomic tube and insert the DSC port 3 Secure the DSC port in place with the DSC port fixing screw using the tool provided with the microscope 4 Connect the camera connection connector for the camera head and the camera connector for the DS L3 DS U3 DS camera control unit using a provided came
48. mount camera ae Camera cable to Various adapters DS U3 or DS L3 DS camera head Photomicrography device FA P Centering telescope CFI eyepiece E TV adapter DSC port for ergonomic Tube adapter for binocular tube quadrocular tube Motorized shutter cable to N R control IN box B y Binocular tube Trinocular tube Quadrocular tilting tube DSC zooming port Motorized shutter Motorized shutter adapter To control box B f T Eyelevel riser US Ra l E C f Epi fluorescence HG adapter ae Epi fluorescence attachment Analyzer unit cube turret 2 h Contact arm Standard arm a O FL DIC Excitation A analyzer balancer HG fiber Analyzer slider EQ Lambda plate gt E DIC Nosepiece Nosepiece to nosepiece with DIC slider analyzer slot 1 CFI objective 12 J T Ey Ni U main body im T J 4 Substage for rotatable ceramic Rotatable ceramic coated stage coated stage IQ EY mn es a Ni F AN L we J Substage Control ES gt box B Stage Condenser Condenser holder Polarizer unit Simple remote control pad 1 A contact arm is required to use a m
49. n A halogen light source is used for a dark specimen Change the light source to a mercury lamp A designated objective is not used at UV or V excitation Use a designated objective The room is bright Make it darker The fluorescent image quality is poor The dia illumination lamp is on Turn off the dia illumination lamp The filter cube being used is not suitable for the specimen Use a filter cube suitable for the specimen gt See Chapter 3 14 2 Selecting Filters in the Operation The objective or cover glass is dirty Clean it as appropriate See Chapter 3 2 1 Cleaning Lenses in the Assembly Maintenance The contrast of the fluorescent image is poor The immersion oil is fluorescent Use the non fluorescent immersion oil gt See Chapter 3 14 7 Other Considerations Concerning Epi Fluorescence Microscopy in the Operation The slide glass is fluorescent Use a non fluorescent slide glass See Chapter 3 14 7 Other Considerations Concerning Epi Fluorescence Microscopy in the Operation Stray light is entering from the condenser Lower the condenser or remove the condenser and attach a shielding tube 30 Chapter 2 Troubleshooting 1 3 Differential Interference Contrast Microscopy Problem Cause Measure Lack of visibility around periphery of field of view The
50. n the front of the arm using Removing the tube tightening screw a hex driver 2 mm across flats so that the tip of the Tube bottom view screw does not protrude into the connecting section c onnection The fixing screw at the front of the turret if fixing screw epi fluorescence cube turret is attached Positioning hole 4 Loosen the fixing screws x2 on both sides of the zooming port with a hex driver 2 mm across flats 5 Align the round dovetail of the quadrocular tube with the round dovetail of the arm and slide the entire attached devices to the rear 6 Tighten the fixing screw loosened in step 3 Connecting the quadrocular tube 7 Tighten the fixing screws x2 on top of the zooming and DSC zooming port port with a hex wrench 3 mm across flats Zooming port 8 Tighten the fixing screws x2 loosened in step 4 fixing screw 9 Affix the provided sticker to cover the hole for the zooming port fixing screw When attaching on the epi fluorescence cube turret the location of the fixing screws at the front is deep so it will be easier that you insert the hex driver 2 mm across flats into the screw before placing the quadrocular tube or that you have a pen light when working Fixing the quadrocular tube and DSC zooming port set Attach the EPI motorized shutter optional The motorized shutter is attached by Nikon Contact your nearest Nikon representative when the EPI motorized shutter needs to be att
51. nce Cube Turret e NI SH E Motorized Shutter e C HGFIE HG Precentered Fiber Illuminator e This product meets FCC Part 15B Class A requirements This equipment has been tested and found to comply with the limits for a Class A digital device pursuant to Part 15 of the FCC Rules These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instruction manual may cause harmful interference to radio communications Operation of this equipment in a residential area is likely to cause harmful interference in which case the user will be required to correct the interference at his own expense e This Class A digital apparatus complies with Canadian ICES 003 Cet appareil num rique de la classe A est conforme a la norme NMB 003 du Canada e This product complies with Australian EMC AS NZS CISPR11 Group 1 Class B CE Marking e This product meets EU IVD Directive requirements e This product meets EU Low Voltage Directive requirements e This product meets EU EMC Directive requirements 43 suonesi oads suonesuoads Chapter 4 Specifications m Control Box B for Nikon Microscope Model NI CTLB Operating conditions Temperature 0 C to 40 C Humidity 60 RH max no condensation Altitude 2000 m max
52. nd decontaminate the microscope and lenses in accordance with the following procedure m Tools used for cleaning e Blower e Soft brush e Soft cotton cloth lens tissue gauze etc e Absolute alcohol ethyl or methyl alcohol medical alcohol e Petroleum benzene use only for cleaning immersion oil N CAUTION Petroleum benzine and absolute alcohol used for cleaning are highly flammable Be careful when handling these materials particularly around open flames or when turning the power switch on or off Follow the instructions provided by the manufacturer when using petroleum benzine or absolute alcohol When cleaning the product do not use organic solvents alcohol ether thinner etc for coated plastic or printed areas It will result in discoloration or peeling of printed characters Petroleum benzine should be used only to wipe off immersion oil from the objective and never to clean the entrance lens at the bottom of the eyepiece tube prism surface of the eyepiece tube or the filters 2 1 Cleaning Lenses Keep the lens free of dust and fingerprints If there is contamination on the lenses or filters image quality decreases If any of the lenses become dirty clean them by following the procedure given below Cleaning light dirt dust 1 Blow dust off using an air blower 2 _ If this is insufficient brush away dust with a soft brush or wipe away gently with a piece of gauze Cleaning tough dirt fingerprint
53. ng tube Attach the tightening screw removed from the bottom of the tube in Chapter 1 3 Assembly Method 7 Attach the quadrocular tilting tube and DSC zooming port for quadrocular tube Set the optical path switching dial to the F position in advance e Manual DSC zooming port Set the zoom dial to 0 6x no tightening screw 4 Storage e Store this product in a dry location where mold is unlikely to form Storage conditions are as follows temperature 20 C to 60 C humidity 90 RH max no condensation e Store the objectives and eyepieces in a desiccator or similar container with a drying agent e Place a cover over this product to protect it from dust e Switch off the microscope press the power switch to the O position and wait for the lamphouse to cool before covering this product with a cover 5 Regular Inspections Charged To maintain the performance of this product Nikon recommends periodic inspections chargeable service Contact your nearest Nikon representative for details 38 Chapter Specifications suonesi oads suonesiuoads Chapter 4 Specifications 1 Microscopy Principles Use objectives and eyepieces of the microscope to magnify minute cells and tissue optically and manipulate levers and knobs of the microscope unit to adjust the focus or move the observation point Then observe or take photographs of the sample fixed on the slide m Intended use of this product for med
54. nto the optical path See Chapter 2 4 Differential Interference Contrast Microscopy 14 Adjust the orientation vibration direction of the polarizer and analyzer in the Operation A correct DIC module on the condenser has not been selected Select the DIC module suitable for the objective used gt See Chapter 1 3 Assembly Method 13 Attach the condenser M Attaching the optical module to the universal condenser and W Attaching the DIC module to DIC condenser oil in the Assembly Maintenance The DIC slider on the objective is out of the optical path Bring the DIC slider on the objective into the optical path See Chapter 2 4 Differential Interference Contrast Microscopy 16 Attach the DIC slider on the objective to the nosepiece in the Operation Incorrect combination of the objective and the DIC slider on the objective Use the DIC slider suitable for the objective gt See Chapter 1 3 Assembly Method 13 Attach the condenser M Attaching the optical module to the universal condenser and W Attaching the DIC module to DIC condenser oil in the Assembly Maintenance The immersion oil is not sufficient in the oil condenser and air bubbles are between the slide and the condenser Apply the immersion oil again gt See Chapter 3 12 Oil Immersion Water Immersion in the Operation 31 Buljooysajqnoi Ch
55. oated stage in Stage positioning on Stage centering screws the Operation on both sides screw When removing the stage Securing the rotatable ceramic coated stage When removing the stage loosen the stage centering screws x2 and stage positioning screw 12 Chapter 1 Assembly Attach the condenser Tool Hex driver 2 mm across flats 1 Turn the coarse focus knob until the substage is brought to the upper limit 2 Turn the condenser focus knob until the condenser holder reaches the bottom 3 Fit the round dovetail of the condenser to the condenser holder so that the Nikon nameplate faces front and tighten the fixing screw of the condenser holder with a hex driver Condenser fixing screw Securing the condenser E Attaching the optical module to the universal condenser The turret has one empty hole and six optical module mounting holes The empty hole is address 1 and the optical module cannot be attached here The desired optical module can be mounted in the mounting holes of address 2 to 7 Tool Hex driver 2 mm across flats e DIC module on the condenser required for differential interference contrast microscopy There are DIC modules D C DIC N1 DRY D C DIC N2 DRY and D C DIC NR DRY Select the DIC module appropriate for the objective See following table If the combination is not correct differential interference contrast image cannot be obtained or the contrast decreases
56. otorized intelligent nosepiece 2 A contact arm is required to use a motorized intelligent epi fluorescence cube turret 3 DS U3 DS camera control unit can not be connected via USB Mercury lamp gt RS 232C cable to control box B for motorized operation Motorized shutter cable to control HG precentered fiber illuminator a boxB To Ni U main body Motorized Motorized shutter adapter i el Lamphouse TT DS L3 DS U3 Camera PC Control Unit USB cable to control box B 3 Camera trigger cable to Ni U main body or control box B Chapter 1 Assembly Components List The ECLIPSE Ni U components are shown in the table below Depending on when you purchased the ECLIPSE Ni U there may be devices not yet available for use and devices not listed that are already available for use Contact your nearest Nikon representative for details Device Device Name Model Remarks Microscope main ECLIPSE Ni U 12v 100W 100 240V power supply ECLIPSE Ni U body for illumination integrated Illumi Precentered lamphouse 12V 100W NI LH luminator for PHILIPS 7724 dia illumination Halogen lamp
57. positioning pin and module fixing screw e First check that the tip of the module fixing screw is not inside the hole If it is inside place that hole into the optical path lift the Ph module centering knob at right rear of the condenser and turn the centering screw to loosen the module fixing screw After it is loose pull out the knob and restore it to the original position e Determine the orientation of the module so that the positioning pin on the hole can be inserted into the groove on the opposite side of the surface with the module name and attach the module As the grooves for the dark field module and 2 4x auxiliary lens are wider compared to the pin they do not need a strict orientation unlike the DIC module on the condenser e Bring the attached hole into the optical path turn the centering screw and tighten the module fixing screw to secure After it is tightened pull out the knob and restore it to the original position Attaching PH module Holes 2 3 and 4 have a spring e First check that the tips of the two Ph module centering screws are not inside the hole If it is inside place that hole into the optical path lift the Ph module centering knob at right and left rear of the condenser and turn the centering screw to loosen the module fixing screw After it is loose pull out the knob and restore it to the original position e Place the module with its name facing up e Place it into the hole by pushing the spring
58. ra cable 5 Connect the EXT I O connector for DS L3 DS U3 with the DSC connector for the microscope or the Ex OUT connector for the connector box B using a camera trigger cable For DS L3 a USB cable can also be used for connecting the USB H connector for DS L3 and the USB connector for the connector box B Precautions for connecting the camera trigger cable When connecting the camera trigger cable with the DSC connector be sure to insert it completely Prior to photomicrography adjust the camera position as appropriate See Chapter 3 18 Capturing Images Photomicroscopy in the Operation 20 IN ZJO PIZA Z Eyelevel riser fixing Eyelevel riser screw Securing the eyelevel riser Attach a camera and connect the DS camera control unit optional 1 When attaching to the DSC port for the ergonomic binocular tube Camera connection connector DSC port DSC port fixing screw Attaching a camera to the DSC port for the ergonomic binocular tube Chapter 1 Assembly 1 2 3 Screw the camera head into the C mount on the DSC port Connect the camera connection connector for the camera head and the camera connector for the DS L3 DS U3 DS camera control unit using a provided camera cable Connect the EXT I O connector for DS L3 U3 with the Ex OUT connector for the microscope or the Ex OUT connector for the connector box B using a camera trigger cable
59. rizer unit Slider Analyzer slider for simple polarization D SA Analyzer slider for first order red compensation C AS DIC slider D C Can be attached to D ND6 NI ND6 I or NI ND6 E nosepiece Lambda plate D LP Can be attached to D ND6 NI ND6 I or NI ND6 E nosepiece DIC rotatable polarizer unit D DP Polarizer unit Polarizer unit for simple polarization C SP Polarizer unit for first order red compensation C TP Motorized shutter NI SH E For connection NI SHCL motorized shutter cable is required and is connected to the control box B Shutter Motorized shutter adapter for upright epi fl NI SHAEP U Motorized shutter adapter for dia illumination NI SHADI Control box B NI CTLB Simple remote control pad NI SRCP Controller DS L3 is connected to the control box B via USB cable e DS L3 DS L3 DS U3 is connected to the Ni U main body or the control box B via C CTC DS L3 DS U3 camera control unit DS U3 camera tri ble L3 U3 gger cable DS camera I F cable is used to connect to the camera Eyelevel riser Eyelevel riser C ER Variable Intermedlate Variable intermediate magnification unit Y IM magnification unit DSC retention DSC support column NRS support DSC adapter A and B NI RPSAB Tube adapter for Tube adapter for quadrocular tube C TAQ Can be attached to the quadrocular tilting tube Attach a C mount camera ENG mount quadrocular tube camera or a photomicrography device via various adapters Y TV can be attached to C TF or C TT trinocular tube Connect a C mount
60. slot cover window Insert into the same address window as the address shown inside the slot Filter cube replacement slot cover Address indication Filter cube Cube switchover turret 4 Turn the cube switchover turret to attach the filter cubes in the remaining slots and also insert the nameplates Address indication Nameplate window For bright field microscopy For bright field microscopy be sure to make the address 1 empty For motorized intelligent epi fluorescence cube Attaching a filter cube turret only address 1 can be set to OPEN 5 Restore the slot cover back to its original position Chapter 1 Assembly E Replacing excitation and barrier filters The excitation filter barrier filter and dichroic mirror can be removed from the cube for replacement Excitation filters are screw in filters while barrier filters are slide in filters Align the projection on the barrier filter with the groove on the filter cube and turn clockwise by approximately 30 degrees to secure it in place O Orientation of the filter The filter is two faced Be aware that the filter does not face a wrong side Nikon filters have an arrow indication printed on the outer frame Make sure that the arrow faces the direction of a dichroic mirror when attaching the filter Contact your nearest Nikon representative when the dichroic mirror needs to be replaced E Attaching a l
61. specified type gt See Chapter 3 1 Replacing the Lamp in the Assembly Maintenance The lamp is not attached Attach a designated lamp gt See Chapter 1 3 Assembly Method 3 Attach the lamp in the Assembly Maintenance m Controls on the main body controller Problem Cause Measure Brightness cannot be adjusted with the dia illumination brightness control knob Operation is disabled Change the setting See Chapter 3 1 1 Adjustment by Lamp Voltage Turning lamp ON brightness adjustment control in the Operation The simple remote control pad does not work to move the motorized unit A contact arm is not used Use a contact arm gt See Chapter 1 3 Assembly Method 5 Attach the standard arm contact arm in the Assembly Maintenance Cables for the contact arm control box and the simple remote control pad are not connected properly Connect the cable properly gt See Chapter 1 3 Assembly Method 20 Connect the motorized device cable in the Assembly Maintenance 33 Bulooysa qno Bunooys jqnoIL Chapter 2 Troubleshooting m Motorized nosepiece Problem Cause Measure Motorized nosepiece does not move at all The motorized nosepiece is attached incorrectly Attach it correctly gt See Chapter 1 3 Assembly Method 14 Attach the nosepiece
62. stage with 1S holder C CSR1S St Left handle ceramic coated stage with 1S holder C CSL1S age x z NI U right handle rotatable ceramic coated stage i NIU CSRR2 with holder NI U left handle rotatable ceramic coated stage with NIU CSLR2 holder Condenser holder Condenser holder NI CH Dark field condenser Oil and Dry C C aplanatic condenser C C achromat condenser No 9 C C abbe condenser No 9 X LWD condenser Condenser C C slide achro condenser 2 100x C C achromat swing out condenser 1 100x C C phase turret condenser Cannot be used when rotatable ceramic coated stage is attached DIC condenser Oil D CUO Used with OIL DIC module Universal condenser Dry NI CUD ee DRY DIC module PH module dark field module NI CALN1 2 4x auxiliary lens D C 2 4x auxiliary lens Epi fluorescence Cube turret NI FLT6 i cube turret Intelligent epi fluorescence cube turret NI FLT6 I A filter cube and NI FA FL DIC analyzer attachable Motorized epi fluorescence cube turret NI FLT6 E Epi fluorescence Epi fluorescence attachment NI FLEI D FB excitation balancer attachable attachment Mercury lamp HG precentered fiber illuminator C HGFI illuminator Motorized HG precentered fiber illuminator C HGFIE Analyzer slider for DIC D DA Can be attached to C NA D ND6 NI ND6 I NI N7 I NI ND6 E or NI N7 E nosepiece Analyzer tube for simple polarization C ISA Combined with C SP polarizer unit Analyzer unit Analyzer tube for first order red compensation C IA Combined with C TP pola
63. the cover glass is inadequate Attach a cover glass of the specified thickness 0 17 mm Fail safe device for specimen damage protection of the objective is pushed in Some objective has a stopper to keep the pushed in state Turn the tip of the object to release If the objective does not have a stopper do not pull because the tip cannot be turned In this case contact your nearest Nikon representative A focal deviation is high when switching over objectives Objective lens is attached incorrectly Screw the objective all the way in gt See Chapter 1 3 Assembly Method 15 Attach the objective in the Assembly Maintenance Diopter adjustment has not been performed Perform diopter adjustment See Chapter 3 4 Adjusting the Diopter in the Operation Image is not in focus although the stage is raised to the highest position The stage is attached incorrectly Attach it correctly See Chapter 1 3 Assembly Method 12 Attach the stage in the Assembly Maintenance The refocusing position is set lower than the focusing position Loosen the coarse focus clamp ring to the limit which clamps the refocusing mechanism gt See Chapter 3 2 3 Refocusing in the Operation 28 Chapter 2 Troubleshooting Problem Cause Measure One side of the field of view up down right or left is not focused The image flows
64. with ND filters See Chapter 3 1 Adjusting the Brightness of a Diascopic Image in the Operation Visually observed image color does not match color of image on monitor White balance of the camera is not set correctly Set the white balance in accordance with the camera s instruction manual The entire field of view is bluish or yellowish A filter cube is in the optical path even though epi fluorescence observation is not being performed Remove the filter cube from the optical path 27 Bunooys jqnoIL Bunooys jqnoIL Chapter 2 Troubleshooting Problem Cause Measure Lack of visibility around periphery of field of view Illumination is uneven across the field of view Field of view is not visible Parts are attached incorrectly Confirm that parts nosepiece condenser etc are correctly attached gt See Chapter 1 3 Assembly Method in the Assembly Maintenance Movable part of manually operated unit is not switched correctly Correctly set the optical path switching knob nosepiece filter cube switchover turret condenser turret and slider etc Move the part until it clicks Field diaphragm image is not focused on the specimen surface Make sure the condenser is focused and centered See Chapter 3 5 Focusing and Centering the Condenser in the Operation Field diaphragm is stopped down too far Open th
65. x B 3 Connect the cable from the CONTACT ARM2 connector to the CONTACT ARM2 connector on the control box B Positioning pin Standard arm Contact cover Arm fixing screw y Contact arm Attaching the arm Chapter 1 Assembly Attach the epi fluorescence cube turret and epi fluorescence attachment required for epi fluorescence microscopy Tool Hex driver 2 mm across flats Hex wrench 3 mm across flats First connect the epi fluorescence cube turret and epi fluorescence attachment and then attach them on the microscope arm 1 Insert the positioning pin of the epi fluorescence attachment while aligning it with the positioning hole on the epi fluorescence cube turret side and then tighten the connection fixing screws x2 on the epi fluorescence cube turret with a hex driver 2 When using a motorized or intelligent epi fluorescence cube turret remove the contact cover on the contact arm beforehand Contact cover is not removed for manual epi fluorescence cube turret 3 Loosen the fixing screw on the front of the arm using a hex driver so that the tip of the screw does not protrude into the connecting section 4 Align the round dovetail and convex contact on the bottom of the epi fluorescence cube turret with the round dovetail and concave contact of the arm and slide the entire mounted attachment to the rear There is no concave contact in manual epi fluorescence cube turret 5

Upright Microscope ECLIPSE Ni-U Instruction Manual

Contents

Download Pdf Manuals

Related Search

Related Contents