User Manual CxL - Labo America, Inc.
Contents
1. 3 8g Monocular WF 10x WF 16x WF 20X n i L l l PN S m LP 4x LP foe LP 20 LP an LP sox T 100x LP 10 Iris LP 10x phase LP 40x phase he SS es SS Y 9o Abbe Condenser Phase Slider Polarizing Kit DF Slider Blue Filter Green Filter ii i i J i L Q gt amp Q Mirror Halogen Bulb LED Monnt Rechargeable Battery jl Lanon Installation and Operation of Optional Accessories Darkfield Slider SS The darkfield slider enables darkfield observation using an objective from 4X to 40X Fig 4 e a Reflection Mirror The reflection mirror is designed to be used in out door observation settings wherein a power source is not readily available The mirror attachment makes it possible to use natural light to illuminate your specimen The microscope should be installed in any place that is not exposed to direct sunlight Direct sunlight reflection can cause eye injury or in some cases blindness The eyepieces should be oriented toward the rear of the microscope so that the reflection mirror receives unobstructed light To do this loosen the observation tube clamping knob and rotate the tube 180 1 Fit the reflection mirror in the window lens of the microscope frame by aligning the mounting tab 2 Remove the daylight filter because it renders the image blue under natural light conditions 3 The ref2. 22 10 5 0 56 0 1 Adjusting the Interpupillary Distance 19 Fig 22 The inter pupillary distance adjustment consists of regulating the two eyepieces to align with both eyes pupils so that you can observe a single microscopic image through two eyepieces in stereo vision This greatly helps to reduce fatigue and discomfort during observation While looking through the eyepieces move both eyepieces until the left and right fields of view coincide completely The position of index dot indicates the inter pupiliary distance value Note your interpupillary distance so that it can be quickly duplicated Procedure for adjusting the diopter 1 Rotate the right eyepiece to match the markings of your IPD If your IPD is 64 rotate the eyepiece to 64 mark 2 While looking through the right eyepiece with your right eye rotate the coarse and fine adjustment knobs to bring the specimen into focus 3 While looking through the left eyepiece with your left eye rotate only the diopter adjustment ring on the eyepiece until specimen is at its best possible focus e This is a special feature provided to compensate the tube length change while changing IPD setting Follow this procedure to obtain optimum parfocality of system to your IPD setting Using the Eye Shades When Wearing Eyeglasses Use with the eye shades in the normal folded down position This will prevent t
3. 4 moves the specimen in the vertical direction Rotating the lower knob which is the X axis movement control Knob 5 moves the specimen in the horizontal direction Do not adjust the specimen holder directly by hand for this will damage the rotary mechanisms When the specimen holder reaches stop position the rotation force of the above knobs become heavy Stop rotating the knob at this time Adjusting the Focus WD Specimen holder scales Theses scales allow for easy identification of the specimen s position coordinates making it easy to return to a particular region of interest after scanning the slide 1 The horizontal coordinate can be read at position 1 on the specimen holder fig 18 2 The vertical coordinate can be read at the index line 2 Focusing Procedure 1 Rotate the coarse adjustment knob 1 clockwise so that the objective 3 is as close as possible to the specimen We recommend starting with 10X See figure 19 2 While observing the specimen through the eyepieces slowly rotate the coarse adjustment knob 1 counterclockwise to lower the stage 3 When coarse focusing of the specimen is obtained an image is detected rotate the fine adjustment knob 2 for fine focusing Working Distance WD The WD refers to the distance between each objective and the specimen when precise focus of the specimen is obtained Objective Magnification 4x 10X 40X 100X WD mm
4. Labomed for assistance Trouble Cause Remedy 1 Uneven brightness in observation field 2 Dustor stains are visible in observation field The objective is not engaged in the light path Engage the objective into position Until the nose turret clicks The condenser is too low Raise up to achieve maximum light The objective eyepiece condenser and or window lens are dirty Clean them thoroughly The eyepiece condenser window lens and or specimen glasses are dirty Clean them thoroughly with lens tissue and alcohol 3 Observation image has glare The condenser is too low The condenser iris diaphragm ring is excessively closed Raise it Adjust the aperture according to the objective magnification 4 Observation image is hazy or unclear The objective is not engaged in the light path Engage the objective into position until it clicks The objective eyepiece condenser and or specimen glasses are dirty Clean them thoroughly Immersion oil is not used with an immersion objective Use immersion oil Bubbles are presentin immersion oil The specified immersion oil is not used Remove the bubbles by agitation Use the immersion oil supplied by Labomed 5 Part of image is defocused The objective is not properly engaged in the light path Engage the objective into position Until the nose turret clicks The specimen is not set properly on the stage S
5. Observation Head as desired Insert the eyepieces into the ocular tube of Observation Head using following procedure 1 Remove the protective caps from the observation tube 2 Insert 10x eyepieces into the ocular sleeve and tighten the clamping screw 1 using allen wrench 1 2mm See figure 10 ASSEMBLY Each standard set can be assembled by simply attaching filter and power cord Installing or Replacing the Bulb Before attaching the bulb remove the parts that may drop such as the filter and specimen from the microscope frame and place the microscope on its back so that the bottom plate is exposed 3 1 Pull the lock knob 1 on the bottom to open lamp housing door fig 11 yo _ 2 2 Hold the halogen bulb 2 without taking it out of the polyethylene bag so as notto taint the bulb with fingerprints and push the bulb into the pin holes on the ocket 3 After attaching remove the polyethylene bag 3 With the lock knob pulled out close the lamp housing door then push the lock knob back to lock the cover i Applicable bulb 6V20W Halogen bulb P N CX 013 Fig 11 Always use the designated bulb Using a bulb other than those specified by LABOMED may lead to a fire hazard Fingerprints or stains on the lamp bulb reduce its life If contamination occurs wipe bulb surface with a cloth slightly moistened with alcohol Caution For Bulb
6. care 2 Do not use the microscope where it is subjected to direct sunlight high temperature humidity dust and vibrations For the operating conditions see chapter 15 SPECIFICATIONS on Page 24 3 The microscope is ventilated by natural convection Be sure to leave enough space 10 cm or more around body while installing it 4 Arm handle is provided for carrying the microscope NTo prevent damage do not hold the microscope by the stage or observation tube Be sure to remove the specimen from the stage clip while transporting unit to avoid damage to the specimen slide Maintenance and Storage 1 Clean all glass components by wiping gently with cleaning cloth provided To remove fingerprints or oil smudges wipe with cleaning cloth slightly moistened with a mixture of petroleum 85 and isopropanol 15 Since solvents such as petroleum and isopropanol are highly flammable they must be handled carefully Be sure to keep these chemicals away from open flames or potential sources of electrical sparks for example electrical equipment that is being switched ON or OFF Also remember to always use these chemicals only in a well ventilated room 2 Do not attempt to use organic solvents to clean the microscope components other than the glass components To clean non glass components use a lint free soft cloth slightly moistened with a diluted neutral detergent 3 Do not disassemble any part of
7. the microscope as this could result in malfunction or mitigated performance 4 When not using the microscope ensure that the frame is fully cooled and store the unit in a dry locker or cover with a dust cover provided 5 To clean the condenser fully loosen the securing knob lower and remove the condenser by lowering condenser bracket wipe the front lens of the condenser with optical cleaning solution mixture suggested above and lens tissue Fig 3 CxL Caution If the microscope is used in a manner not specified by this manual the safety of the user may not be warranted In addition the equipment may also suffer damage Always use the equipment as outlined in this instruction manual Care amp Maintenance Your microscope has been engineered for a long and safe operational life with the least amount of maintenance required In general routine maintenance is limited to keeping the microscopes working parts lubricated and optics clean Always cover the microscope with the provided dust cover when notin use 1 Cleaning the lenses To clean the lens surfaces remove dust using a soft brush or gauze compressed air dust cans are ideal For removing finger marks or grease soft cotton cloth lens tissue or gauze lightly moistened with cleaning solution 85 petroleum ether and 15 isopropanol should be used For cleaning the objective optics use xylene Observe sufficient caution in handling xylene Cleaning procedure Pl
8. very lightly moistened micro fiber cloth Loose dust and other dirt can be removed using a brush of soft hair used exclusively for this purpose A Caution Do not use aggressive organic solvent such as acetone for cleaning painted surfaces and plastic parts of the microscope 3 Never attempt to dismantle Never attempt to dismantle the instrument so as to avoid the possibility of impairing its operational efficiency and accuracy 4 Periodical checking To maintain the performance of the instrument we recommend customers have their microscopes serviced periodically by a factory authorized dealer rep For details contact your nearest dealer or Labo America s main office in California PA CxL lt 2 CxL Monocular Monocular viewing tube Revolving nosepiece Objectives Mechanical stage Coarse and fine focus knob Light regulator X Y movement control knob CxL Binocular Binocular viewing tube Revolving nosepiece Objectives Mechanical stage Coarse and fine focus knob Light regulator X Y movement control knob CxL CxL Trinocular lt gt Port for camera adapter Focusable eyepieces Trinocular viewing tube e Revolving nosepiece Objectives _ Mechanical stage ZS LEE o Abbe condenser
9. will be charged with a direct input power supply of 110V 240V AC 50Hz 60Hz This ensures continuous operation even under fluctuating voltages Our Halogen bulb 6V 20W on this instrument has an average life span of up to 2000 hours and LED has an average life span of up to 100 000 hours The CxL comes equipped with a removable N A 1 25 Abbe condenser for brighter illumination levels and an iris diaphragm for better resolution and contrast control 1 After the microscope has been used for observation of a specimen containing bacteria clean all parts coming in contact with the specimen to prevent infection E Be sure to remove the specimen before moving this product E In case the specimen is damaged by erroneous operation it is important to clean all surfaces that may have come in contact with the specimen 2 To avoid potential shock hazards and burns when replacing either halogen bulb or LED turn the microscope s main switch to OFF position and disconnect power cord from wall outlet in advance Whenever you replace bulb during use or right after use allow lamp socket and bulb to cool before touching Fig 1 Applicable bulb LED replacement 6V20W Halogen bulb P N CX 013 or LED P N 9135000 901 3 Install microscope on a sturdy level table or bench and avoid any blockage of air vents on the bottom side of the base Do not place microscope on a flexible surface as this could result in blocking the air vents and
10. Engage the objective to be used in the light path and bring the specimen in focus Engage the required filters Adjust the brightness Observe Specimen 16 17 am La d y lt i a X Turning the Lamp ON NWS Fig 15 Placing specimen on the stage gt DETAILED OBSERVATION PROCEDURE Flip the main switch to I ON as shown in figure 14 Rotating the light intensity adjustment knob fig 15 in the direction of the arrow increases brightness and rotating it in the opposite direction decreases brightness The figures around the knob indicate the intensity lever Place the specimen gently on the stage If the bow shaped lever 3 is returned with a strong force or the control knob 1 or the bow shaped lever is released in the middle the slide glass may be broken See figure 16 1 Rotate the coarse adjustment knob 2 in anticlockwise direction to fully lower the stage Open the bow shaped lever 3 outward by pulling lever handle 1 place the specimen by sliding the specimen glass plate s on the stage from the front toward the rear After positioning your specimen slides return the bow shaped lever 3 gently by slowly releasing control knob 1 Rotating the upper co axial knob controlling the Y axis movement
11. N LABOMED CxL User Manual Laboratory Microscopy To ensure proper use of this instrument as well as to avoid injury while operating instrument understanding this manual completely before use is highly recommended CONTENTS INTRODUCTION The CxLis alaboratory microscope reflecting a modern design as well as the latest in optical and mechanical advancements Designed for professionals as well as students this microscope offers many features and functions for a diverse set of applications Extra clarity and contrast is provided through a 360 rotatable Binocular body inclined at 45 The pressure die cast stand consists of Ball bearing friction less sideways focusing to avoid any loss in motion The sturdy new stylish design provides comfort as well as stability The high powered objectives are spring loaded to prevent accidental damage to specimen slides The quadruple nosepiece has a comfortable ribbed grip for easy rotation that also safeguards the turret system against any damage All positions are par centered and par focalised ensuring the highest level of accuracy The ball bearing mechanical stage allows smooth travel over a 76 x 50mm area with spring loaded stage clamps for retaining the specimen at the exact position desired AO 1mm vernier scale provides accurate location of specimen area The LED configuration is operational with an in built re chargeable battery and charging circuit The battery
12. Q f h Coarse and fine focus knob Light regulator X Y movement control knob tABomep UNPACKING YOUR MICROSCOPE Mirror attachment if ordered Eyepieces Camera if ordered Observation tube Software CD if ordered 9 Microscope Arm CxL SYSTEM DIAGRAM Focusable Eyepieces Observation tube clamping Revolving nosepiece screw objectives Specimen holder Mechanical stage Coarse and fine focus knob X Y stage movement control knob 10 STANDARD COMPONENTS After removing microscope from packaging make sure that all of the following contents are present The differences in configurations are the number of objectives type of observation head type of illumination and purchased optional accessories The objectives have been adjusted for a tight fit to prevent any damage during transportation To remove an objective rotate it counterclockwise while gripping it with a rubber sheet etc to avoid any slippage Stage Condenser i NSL d Daylight blue filter Power Cord wy SY Sn Allen Wrench 3mm Allen Wrench 1 2mm 6v 20w Halogen bulb Spare fuses 11 CxL Video adapter Photo adapter Photo eyepiece DSC adapter iVU 1500 L L L L J
13. Replacement During Use or Right After Use The bulb lamp socket and areas near these will be extremely hot during and right after use Set the main switch to O OFF disconnect the power cord from the wall outlet and allow the bulb and lamp socket to cool before replacing the bulb with a new bulb of the designated type Cooling time may vary to users discretion Procedure for LED replacement figure 12 1 Lay the microscope on a clean surface toward right so that the microscope base is exposed 2 Using a screw driver unscrew four screws 1 to open the bottom plate of the microscope 3 Remove two screws 2 provided on the lamp housing use screw driver 4 Replace existing LED mount with live LED mount 5 Reverse the steps 3 to 1 to complete the process Fig 12 Mounting the Daylight Blue Filter This filter modifies the color of observation light into a natural color a daylight color 2 e Fit the daylight filter 1 into the bottom of the condenser 2 until it clicks into place See figure 13 1 Fig 13 CXL Flip the main switch to ON l Place the specimen on the stage Engage the 10X objective in the light path Main switch Bring the specimen in focus eyepieces Adjust the observation tube and Adjust the interpupillary distance Adjust the dioptric setting Adjust the aperture iris diaphragm
14. ace the objectives and or eyepieces on a dust free surface e g fresh aluminum foil All other optical components to be cleaned should be as accessible as possible a Blowallloose dust particles away with a dust blower b Remove all water soluble dirt with distilled water If this is unsuccessful repeat using a solution of diluted hand soap liquid Remove any remaining residue with a dry cotton swab c To remove oil use a solution of diluted hand soap liquid initially If this does not produce a satisfactory result repeat the cleaning using a solvent Optical Cleaning Solution 85 petroleum ether and 15 isopropanol d Grease must always be removed using a solvent e Cleaning is achieved by using a spiral motion from the center to the rim Never wipe using zig zag movements as this will only spread the dirt With larger optical surfaces e g tube lenses the spiral motion starts initially at the rim before moving to the middle and is only then followed by a center to rim cleaning motion Normally several spiral wipes are recommended We recommend pure volatile petroleum ether or Optical Cleaning Solution as explained in point 3 above zig zag motion X spiral motion v Wipe using a spiral movement Do not use a zig zag motion 2 Cleaning of painted surfaces Avoid the use of any organic solvent e g thinner xylene ether alcohol etc for cleaning of painted surfaces of the instrument Painted surfaces can be cleaned with a
15. ace with live fuse Battery is low Charge battery 11 Bulb LED blows easily The specified bulb LED is not used Replace with a specified bulb LED SPECIFICATIONS CXL 1 Illumination Built in illumination system LED Halogen 2 Focusing mechanism Stage height adjustment mechanism Fine adjustment scale 3 0um per graduation Fine adjustment stroke 0 2mm per turn Total stroke 12 7mm Co axial coarse and fine focusing on ball drive 3 Revolving nosepiece Quadruple positions fixed front oriented 4 Observation tube Monocular Binocular Trinocular Field number 18 18 18 Tube tilting angle 45 45 45 Interpupillary distance NA 54 74 54 74 adjustment range 5 Stage Size 135 x 124mm with mechanical stage Movement range 76 x 50mm Specimen holder Holds a single specimen 6 Condenser Type Abbe condenser daylight filter detachable N A 1 25 Aperture iris diaphragm Built in 7 Dimensions 255 0mm L x 227 0mm W x 375 23mm H 8 Electrical Battery 7 4V 1000mAH Charging time up to 5 hours with totally consumed battery Back up time up to 4 hours 9 Operating environment Indoor use Altitude Max 2000 meters Ambient temperature 5 to 40 C 41 to 104 F Maximum relative humidity 80 for temperature up to 31 C 88 F decreasing linearly through 70 at 34 C 93 F to 50 relative humid
16. cause overheating fire 4 Always use the power cord provided by LABOMED If the proper power cord is not used product safety performance cannot be warranted 5 When installing the microscope route the power cord away from the microscope frame Should the power cord come in contact with the microscopes base the power cord could short circuit 6 Always ensure that the grounding terminal of the microscope and that of the wall outlet are properly connected If the equipment is not grounded LABOMED can not warrant the electrical safety performance of the equipment 7 Never allow metallic objects to penetrate the air vents of the microscope frame as this could result in user injury and equipment damage 8 After operation of microscope be sure to disconnect power cord from connector on the microscope or from the wall power outlet Warning Label A warning indication label is attached to every part where special precaution is required while handling and using the microscope Always read the warnings Warning label Bottom of microscope Warning against high temperature in position frame bulb LED compartment Warning against risk of electric shock J Warning against damage in non A compliance with instructions manual If the warning label is stained or peeled off contact your LABOMED distributor Getting Ready 1 Amicroscope is a precision instrument with delicate glass components Please handle with
17. et the specimen correctly on the stage and secure using the specimen holder 6 High magnification objective touches specimen just before coming into focus The specimen is upside down Set the specimen correctly with the cover glass on the top 7 Coarse focus adjustment cannot lower the stage low enough The condenser is too low Raise the condenser 8 Fields of view of two eyes do not match The interpupillary distance is not adjusted properly IPD compensation for the two eyes is not set The left and right eyepieces are of different magnification Adjust IPD Adjustit correctly Replace one of them so that the left and right eyepieces are identical Trouble Cause Remedy 9 Objective hits the specimen when an objective is switched to a higher magnification objective The specimen is upside down Set the specimen correctly with the cover glass on the top The cover glass is too thick Use a cover glass with thickness of 0 17mm The stage is raised too high Lower the stage The slide is slipped from the slide holder Re position the slide in the slide holder Slide is of excessive thickness Use slide having thickness between 0 9 and 1 4mm 10 Bulb LED does not light Bulb LED is not mounted Attach a bulb LED Bulb LED is blown Replace the bulb LED The power cord is unplugged Plug it securely Fuse is blown Check and repl
18. g first the 10x then 40x objective LSS EA 2 Disengage the 40x cycling towards 100x and place a drop of immersion oil on the center point of the specimen 3 Rotate the revolving nosepiece to engage the immersion objective and rotate the fine adjustment knob to bring the specimen into focus Since air bubbles in the oil will affect the image quality make sure that the oil Is free of bubbles To remove bubbles rotate the revolving nosepiece slightly to agitate the oil 4 The condenser of this microscope manifests the full performance when oil is placed between the slide glass and the front lens of condenser If oil is not placed there the observed image may appear dark 5 After use remove oil from the objective front tens by wiping with lens tissue slightly moistened with petroleum 85 and isopropanol 15 mixture Caution If immersion oil makes contact with your eyes rinse eyes out thoroughly with fresh water If immersion oil makes contact with skin wash affected areas with soap and water If prolonged discomfort is experienced consult your physician immediately CxL Ji 1A IT TROUBLESHOOTING GUIDE Under certain conditions performance of the unit may be adversely affected by factors other than defects If problems occur please review the following list and take remedial action as needed It you cannot solve the problem after checking the entire list please contact
19. he eyeglasses from being scratched When Not Wearing Eyeglasses Extend the folded eye shades outwards direction of the arrow to prevent extraneous light from entering into your line of vision CxL 6 Adjusting the Condenser Position and Aperture Iris Diaphragm SS The condenser is most often used in the highest position If the observed 2 lo i field of view is not uniform enough it may be improved by lowering the i condenser slightly 2 Rotate the condenser height adjustment knob 1 in clockwise direction to move the condenser to the highest position A im Slide the aperture iris diaphragm 2 so that the aperture of the objective in L use is fully illuminated Fig 23 00 1 Switching the Objectives Hold and rotate the revolving nosepiece 1 so that the objective to be used is in line above the specimen Always use the ribbed grip to rotate the objective nosepiece Fig 24 g Using the 100X Immersion Objective The designated immersion oil should be in contact with the top lens of the 100X immersion objective If not the specimen will appear distorted and dull Itis recommended that LABOMED immersion oil is always used Immersion Process ZF 1 Bring the specimen in focus usin
20. ity at 40 C 104 F Supply voltage fluctuations Not to exceed 10 of the normal voltage Pollution degree 2 in accordance with IEC60664 Installation Overvoltage category II in accordance with IEC60664 o_o 24 www laboamerica com Our policy is one of continuous development Labo America Inc reserves the right to change design and specifications without prior notice Labo America Inc U S A 920 Auburn Court Telephone 510 445 1257 Fremont Fax 510 991 9862 CA 94538 sales laboamerica com LABOMED and CxL are registered trademarks of Labo America Inc With a policy of continuous development Labo America Inc reserves the right to change design and specifications without prior notice 2009 Labo America Inc 9135000 990A 12 2009 Printed in U S A
21. lection mirror fig 5 should be pointed toward anywhere with stray light While observing the image through the eyepieces adjust the orientation of the reflection mirror to capture stray light A planar reflection minor is used normally However if the image brightness is uneven or the outside view is visible in the image use a concave reflection mirror The standard 10X eyepieces are clamped using screws 1 Using allen wrench 1 2mm loosen the clamping screw 1 from the ocular tube and remove it 2 Insert the optional eyepieces into the ocular sleeve and tighten the clamping screw 1 13 anarv CxL INITIAL SET UP Objectives are factory set Objectives are par centered and par focalised during assembly phase All objectives have been secured for a tight fit to prevent them from coming loose during transit To remove an objective rotate it counterclockwise while holding it with a rubber sheet etc to avoid any slippage Fig 7 Install the observation head using following procedure 1 Using allen wrench 3mm loosen the Head Locking Screw 1 and remove the dust cover cap provided in dovetail cavity as well as on observation head dovetail 2 Mount the Observation Head by engaging the dovetail provided at the bottom of the head into the dovetail cavity provided in the microscopes arm 3 Tighten the Head Locking Screw 1 after positioning the
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