GeneAmp® PCR System 9700 - Thermo Fisher Scientific
Contents
1. Setpoint Value 94 C 37 C Al A6 A10 B3 C5 C10 D1 D7 E8 F1 F5 F10 0 5 mL Sample Block Module 1 39 1 40 0 5 mL Sample Block Module Index Numerics 0 5 mL Probe Assembly setting up Calibration Verification Test 1 21 Temperature Non Uniformity Test 1 27 0 5 mL Sample Block Module cleaning the heated cover cleaning the sample block wells 1 19 diagrams attached to 9700 Base Module 1 10 cleaning position 1 18 reaction tube frame configuration top and bottom views heated cover 1 12 installing 1 15 overview 1 10 part number 1 13 Peltier heating cooling unit sample block 1 11 modes 1 11 0 5 mL Sample Block Module Temperature Verification Kit part number 1 13 96 Well Sample Block Module part number 1 13 1 18 1 16 1 14 1 12 B block control mode 1 11 burns warning symbol 1 2 C Calibration Verification Test 1 20 1 25 0 5 mL Probe Assembly setting up 1 21 data sheet 1 37 evaluating the results 1 25 required equipment 1 20 running the test 1 23 1 25 cleaning DNA contamination heated cover 1 19 sample block wells heated cover 1 18 position 1 18 sample block wells 1 19 Communautes Europeennes CE Compliance 1 5 configuration 1 19 reaction tube frame 1 16 cooling information 1 12 verifying cooling system customer support e mail address 1 8 help 1 6 1 9 internet address 1 6 regional sales offices 1 8 1 9 telephone fax U S 12. The system 9700 evaluates the uniformity of the sample block temperature for the setpoint values you entered and displays the results A summary screen appears at the conclusion of the test Well C5 F1 F2 F3 F4 F5 If you entered values on the Temperature Non Uniformity Test Data Sheet compare those values with the actual test results 10 Press Accept To interpret the results see Evaluating the Results on page 1 33 11 When you have completed all measurements be sure to Press Cancel Remove the 0 5 mL Probe Assembly from the sample block Turn off the digital thermometer and clean off the oil Remove the 0 5 mL Thermal Insulation Frame from the sample block IMPORTANT Make sure the sample block is at room temperature 25 C before removing the frame 1 32 0 5 mL Sample Block Module Evaluating the When the system 9700 completes the Temperature Non Uniformity Test the TNU Results Performance screen appears See the table below to evaluate the results If the Then Temperature of the sample block wells is uniform Pass appears after each setpoint temperature TNU Performance TNU at 94 C is xx xx Pass TNU at 37 C is xx xx Pass Fl F2 F3 F4 F5 Temperature variation of the sample block wells exceeds performance specifications Fail appears after the setpoint temperature s for which the test failed TNU Performance
3. 508 383 7855 Peptide and Organic Synthesis Press FAX 31 650 638 5981 Protein Sequencing Press FAX 32 650 638 5981 Chemiluminescence Telephone FAX 1 800 542 2369 781 275 8581 U S only or Tropix 1 781 271 0045 9 00 a m to Tropix 5 00 p m ET POMS Telephone FAX 1 800 952 4716 650 638 6223 9 00 a m to 5 00 p m PT Documents on Free 24 hour access to Applied Biosystems technical documents including MSDSs is Demand available by fax or e mail You can access Documents on Demand through the internet or by telephone If you want to order Then through the internet Use http www appliedbiosystems com techsupport You can search for documents to order using keywords Up to five documents can be faxed or e mailed to you by title by phone fromthe a Call 1 800 487 6809 from a touch tone phone Have your fax United States or number ready Canada b Press 1 to order an index of available documents and have it faxed to you Each document in the index has an ID number Use this as your order number in step d below C Call 1 800 487 6809 from a touch tone phone a second time d Press 2 to order up to five documents and have them faxed to you 0 5 mL Sample Block Module 1 7 If you want to order Then by phone from outside the United touch tone phone States and Canada a Dial your i
4. 270 8288 Belgium Tel 32 0 2 712 5555 Fax 32 0 2 712 5516 Italy Milano Tel 39 0 39 83891 Fax 39 0 39 838 9492 Czech Republic and Slovakia Praha Tel 420 2 61 222 164 Fax 420 2 61 222 168 The Netherlands Nieuwerkerk a d IJssel Tel 31 0 180 331400 Fax 31 0 180 331409 Denmark Naerum Tel 45 45 58 60 00 Fax 45 45 58 60 01 Norway Oslo Tel 47 23 12 06 05 Fax 47 23 12 05 75 1 8 0 5 mL Sample Block Module Europe Finland Espoo Tel 358 0 9 251 24 250 Fax 358 0 9 251 24 243 Poland Lithuania Latvia and Estonia Warszawa Tel 48 22 866 40 10 Fax 48 22 866 40 20 France Paris Tel 33 0 1 69 59 85 85 Fax 33 0 1 69 59 85 00 Portugal Lisboa Tel 351 0 22 605 33 14 Fax 351 0 22 605 33 15 Germany Weiterstadt Tel 49 0 6150 1010 Fax 49 0 6150 101 101 Russia Moskva Tel 7 095 935 8888 Fax 7 095 564 8787 Spain Tres Cantos Tel 34 0 91 806 1210 Fax 34 0 91 806 1206 South Africa Johannesburg Tel 2711 478 0411 Fax 27 11 478 0349 Sweden Stockholm Tel 46 0 8 619 4400 Fax 46 0 8 619 4401 United Kingdom Warrington Cheshire Tel 44 0 1925 825650 Fax 44 0 1925 282502 Switzerland Rotkreuz Tel 41 0 41 799 7777 Fax 41 0 41 790 0676 South East Europe Zagreb Croatia Tel 385 1 34 91 927 Fax 385 1 34 91 840 Middle Eastern
5. C Enter actual block temperature xx x Fl F2 E3 F4 F5 Note The digital thermometer displays a four digit value round this off to three digits before typing it in the Calibration Verification screen Note If desired record this value on the Calibration Verification Test Data Sheet page 1 38 to keep a permanent record of the test Press ENTER The system 9700 automatically begins the second reading 45 C setpoint The Calibration Verification screen appears with the setpoint value displayed Calibration Verification Block temp xx x C Cover temp xxx C Setpoint is 45 C Cover must be within 1 C of 105 C Fl F2 F3 F4 F5 Note The cover must be within 1 C of 105 C Repeat steps 3 and 4 for the second reading The system 9700 evaluates the calibration of the sample block temperature for the setpoint values you entered and displays the results A summary screen appears at the conclusion of the test Calibration Verification Actual temperature at 85 C Actual temperature at 45 C Fl F2 F3 FA F5 If you entered values on the Calibration Verification Test Data Sheet compare those values with the actual test results Press Accept To interpret the results see Evaluating the Results on page 1 26 0 5 mL Sample Block Module 1 25 To run the Calibration Verification Test continued Step Action 9 When you have completed all measurements be sure to
6. Press Exit Remove the 0 5 mL Probe Assembly from the sample block Turn off the digital thermometer and clean off the oil Remove the 0 5 mL Thermal Insulation Frame from the sample block IMPORTANT Make sure the sample block is at room temperature 25 C before removing the frame Evaluating the When the system 9700 completes the Calibration Verification Test one of two screens Results appears See the table below to evaluate the results If the sample block module Then the Is properly calibrated Calibration Verification screen appears with the following message displayed Calibration Verification Calibration is good F1 F2 F3 F4 F5 Does not pass the Calibration Verification screen appears with the following Calibration Verification message displayed Test Calibration Verification Instrument may require servic Contact Applied Biosystems Technical Support F1 F2 F3 F4 F5 Ifthe test fails repeat the procedure to make sure the meter was not misread or that errors were not made entering data Ifthe test fails again contact Applied Biosystems Technical Support See Technical Support on page 1 6 1 26 0 5 mL Sample Block Module Running the Temperature Non Uniformity Test Overview Use this test to verify the temperature uniformity of the GeneAmp PCR System 9700 0 5 mL Sample Block Module Equipment Required To complete the Temperature Non Uniformity Test yo
7. STD X X Sec F1 F2 F3 FA F5 Check your Cycle Test results against the passing ranges listed below Average Cycle Time 125 seconds Cycle Time STD 5 seconds 9 If the test fails repeat the procedure once If the test fails again contact Applied Biosystems Technical Support See Technical Support on page 1 6 0 5 mL Sample Block Module 1 37 Data Sheet Calibration Verification Test Instructions When running the Calibration Verification Test record the setpoint values for well A6 on this data sheet At the end of the Calibration Verification Test check the values displayed on the system 9700 against the values recorded here This will help maintain accurate test records Note If desired you may photocopy this page Setpoint Value Well A6 Date Tested By Probe Serial No Meter Serial No 85 C 45 C 1 38 0 5 mL Sample Block Module Data Sheet Temperature Non Uniformity Test Instructions When running the Temperature Non Uniformity Test record the setpoint values for the wells listed on this data sheet At the end of the Temperature Non Uniformity Test check the values displayed on the system 9700 against the values recorded here This will help maintain accurate test records Note If desired you may photocopy this page Date Tested By Probe Serial No Meier Serial No
8. TNU at 94 C is xx xx Fail TNU at 37 C is xx xx Fail F1 F2 F3 F4 F5 Ifthetestfails repeat the procedure to make sure the meter was not misread or that errors were not made entering data If the test fails again contact Applied Biosystems Technical Support See Technical Support on page 1 6 0 5 mL Sample Block Module 1 33 Running System Performance Diagnostics Overview After you have configured the GeneAmp PCR System 9700 conduct the System Performance Diagnostics to verify the integrity of the cooling and heating system There are two System Performance Diagnostics Rate Test Cycle Test Equipment Required These diagnostics require GeneAmp 0 5 mL Thermal Insulation Frame Running the Rate Use the Rate Test to verify that the Peltier units are operating correctly The test takes Test approximately 10 minutes to run To run the Rate Test Step Action 1 Turn on the system 9700 The main menu appears 2 Press Util The Utilities screen appears Note To press the menu items use the corresponding F keys For example Util is F4 on this menu Press Diag The Diagnostics screen appears Press System The System Performance screen appears System Performance Rate Cool and Heat Rate Test Cycle Cycle Performance Test Rete Cycle Fl F2 F3 FA F5 Press Rate from the System Performance screen WARNING Install the appropriat
9. with grounding conductor disconnected AVERTISSEMENT Debrancher le cordon d alimentation avant d ouvrir la continuite des masses est essentielle Pour un fonctionnement sans danger Ne jamais utiliser l equipment si le fil de terre n est pas raccorde WARNING Hot Surface Use care when working around this area to avoid being burned by hot components gt gt Attention Surface chaude 1 2 0 5 mL Sample Block Module Operating Precautions Precautions The following precautions should be taken whenever you operate the system 9700 with the 0 5 mL Sample Block Module installed Note This instrument is able to withstand transient overvoltage according to Installation Category Il as defined in IEC 1010 1 General Use CAUTION The instrument should be used according to the instructions provided in this manual If used otherwise the protection provided by this instrument may be impaired gt Environment Humidity and Temperature CAUTION This instrument is designed for indoor use CAUTION Do not operate in a Cold Room or a refrigerated area The system 9700 will operate safely when the ambient temperature is 5 40 C 41 104 F The system 9700 will meet performance specifications when the ambient temperature is 15 30 C and the ambient relative humidity is 20 80 These specifications have been calculated for altitudes between 0 and 2 000 meters CAUTION This instrument is
10. www address electrical 1 1 Applied Biosystems 1 6 non electrical 1 2 FAX on Demand 1 7 System Performance Diagnostics required equipment 1 33 system performance tests running 1 33 1 36 Cycle Test running 1 35 1 36 Rate Test running 1 33 1 34 T technical support 1 6 1 9 e mail address 1 8 internet address 1 6 regional sales offices 1 8 1 9 telephone fax U S 1 6 Temperature Non Uniformity Test 1 26 1 32 data sheet 1 38 evaluating the results 1 32 required equipment 1 26 running the test 1 29 1 31 tests Calibration Verification Test 1 20 1 25 0 5 mL Probe Assembly setting up 1 21 evaluating the results 1 25 required equipment 1 20 running the test 1 23 system performance 1 33 1 36 Cycle Test running 1 35 1 36 Rate Test running 1 33 1 34 System Performance Diagnostics required equipment 1 33 Temperature Non Uniformity Test 1 26 1 32 0 5 mL Probe Assembly setting up 1 27 evaluating the results 1 32 required equipment 1 26 running the test 1 29 TmpVer soft key using to run Calibration Verification Test 1 22 TmpVer soft key using to run Temperature Non Uniformity Test 1 28 tubes part number 1 13 V voltage quality 1 4 www appliedbiosystems com Worldwide Sales and Support Applied Biosystems vast distribution and service network composed of highly trained support and applications personnel reaches 150 countries on six continents For sales office locations and technica
11. 2 different wells in the sample block Note If necessary press Cancel to exit the test To run the Temperature Non Uniformity Test Step Action 1 Complete the procedures in Setting Up the 0 5 mL Probe Assembly on page 1 28 2 Press Run This starts the Temperature Non Uniformity Test Note To press the menu items use the corresponding F keys For example Run is F1 on this menu The TNU Performance screen appears with the setpoint value displayed TNU Performance Sample temp xx x C Cover temp xxx C Setpoint is 94 C Sample must be within 1 0 C of setpoint Fl F2 F3 FA F5 Note The sample block must be within 1 0 C of the setpoint In addition the cover must be within 1 C of 105 C It may take several minutes for the system 9700 to ramp up The TNU Performance screen counts down the time until the setpoint is stabilized TNU Performance Sample temp xx x C Cover temp xxx Stabilizing at setpoint x xx Fl F2 F3 FA F5 When the Stabilizing at setpoint value decrements to zero read the digital thermometer Note Refer to the instructions included with your Temperature Verification Kit for a detailed description on operating the digital thermometer 1 30 0 5 mL Sample Block Module To run the Temperature Non Uniformity Test continued Step Action 4 Using the numeric keys type the value displayed on the digital thermometer i
12. 6 Cycle Test running 1 35 1 36 1 33 D data sheets Calibration Verification Test Temperature Non Uniformity Test 1 38 diagnostics 1 33 1 36 Cycle Test running 1 35 1 36 Rate Test running 1 33 1 34 digital thermometer 1 21 1 27 Calibration Verification Test Temperature Non Uniformity Test 1 26 DNA contamination heated cover 1 19 sample block wells 1 19 Dual 384 Well Sample Block Module part number 1 13 1 37 1 20 E electrical safety testing 1 2 electrical symbols 1 1 e mail address for technical support 1 8 F FAX on Demand FCC compliance frame configuration part number 1 7 1 5 1 16 1 13 G GeneAmp 0 5 mL Thermal Insulation Frame part number 1 13 GeneAmp Thin Walled Tubes with Flat Caps part number 1 13 grounding and electrical safety 1 4 H heated cover about 1 12 cleaning 1 18 DNA contamination 1 19 heating and cooling information 1 12 help e mail address 1 8 internet address 1 6 regional sales offices 1 8 1 9 telephone hours 1 6 telephone fax U S 1 6 I input output connections 1 4 installing the sample block module 1 15 instrument labels 1 2 interchangeability sample block modules internet address FAX on Demand 1 7 1 10 L loading samples 1 16 1 17 M maintenance routine 1 5 N non electrical symbols 1 2 O operating precautions 1 3 1 5 Communautes Europeennes CE Compliance 1 5 environment humidity and temperature 1 3 FCC complianc
13. Countries and North Africa Monza Italia Tel 39 0 39 8389 481 Fax 39 0 39 8389 493 Africa English Speaking and West Asia Fairlands South Africa Tel 27 11 478 0411 Fax 27 11 478 0349 All Other Countries Not Listed Warrington UK Tel 44 0 1925 282481 Fax 44 0 1925 282509 Japan Japan Hatchobori Chuo Ku Tokyo Tel 81 3 5566 6100 Fax 81 3 5566 6501 Eastern Asia China Oceania Australia Scoresby Victoria Malaysia Petaling Jaya Tel 61 3 9730 8600 Tel 60 3 758 8268 Fax 61 3 9730 8799 Fax 60 3 754 9043 China Beijing Singapore Tel 86 10 6238 1156 Tel 65 896 2168 Fax 86 10 6238 1162 Fax 65 896 2147 0 5 mL Sample Block Module 1 9 Eastern Asia China Oceania Hong Kong Tel 852 2756 6928 Fax 852 2756 6968 Taiwan Taipei Hsien Tel 886 2 2698 3505 Fax 886 2 2698 3405 Korea Seoul Tel 82 2 593 6470 6471 Fax 82 2 593 6472 Thailand Bangkok Tel 66 2 719 6405 Fax 66 2 319 9788 1 10 0 5 mL Sample Block Module About the 0 5 mL Sample Block Module Overview Diagram The GeneAmp PCR System 9700 amplifies nucleic acids using the polymerase chain reaction PCR process The 0 5 mL Sample Block Module attaches to the top of the GeneAmp PCR System 9700 Base Module and allows you to perform up to 60 reactions You can remove the 0 5 mL Sample Block Module and replace it wit
14. Frame Configuration 1 17 Loading Samples into the Reaction Tubes 1 17 Placing the Reaction Tubes into the Sample Block 1 18 Cleaning the Sample Block Module KK KK KK KK KK KK KK KK KK KK KK KK KK KK KK 1 19 Cleaning Overview vehe E RR EK are Pr Ra ey tea eR kr 1 19 Cleaning Position sk kans r A ek Waa wpLEE MEER SE Skea A MA RS 1 19 Cleaning the Heated Cover 0 0 y P le eel E e ean AAA eee SAD Nik 1 19 Cleaning the Sample Block Wells 0 2 0 0 0 cece KK KK KK KK KK 1 20 Running the Calibration Verification Test 1 21 OVELVICW 2660305 5s Ch ORG OEE S cope mo ARS Cat at VES dete 1 21 Equipment Required x gt cc seek RR XWER eed Be Oe eae Beas Ries Eee AW 1 21 Setting Up the 0 5 mL Probe Assembly 1 22 Configuring the System 9700 ee 1 23 Running the est IN s oe oae eX E CR CURE C PR cx De d QUSS RU RC ROUTE GR 1 24 Evaluating the Results e a a e a a E R III 1 26 Running the Temperature Non Uniformity Test 1 27 OVELVICW sus s Aile ey uc n kaba as A ued ba Ea GAN ER RE nik E os 1 27 Equipment Required ccur ai e e oea e 1 27 Setting Up the 0 5 mL Probe Assembly 1 28 Configuring the System 9700 2 2 KK KK KK KK KK KK KK e
15. GeneAmp PCR System 9700 0 5mL Sample Block Module User s Manual KS Bi D tems Copyright 2006 2010 Applied Biosystems All rights reserved Information in this document is subject to change without notice Applied Biosystems assumes no responsibility for any errors that may appear in this document APPLIED BIOSYSTEMS DISCLAIMS ALL WARRANTIES WITH RESPECT TO THIS DOCUMENT EXPRESSED OR IMPLIED INCLUDING BUT NOT LIMITED TO THOSE OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE IN NO EVENT SHALL APPLIED BIOSYSTEMS BE LIABLE WHETHER IN CONTRACT TORT WARRANTY OR UNDER ANY STATUTE OR ON ANY OTHER BASIS FOR SPECIAL INCIDENTAL INDIRECT PUNITIVE MULTIPLE OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT INCLUDING BUT NOT LIMITED TO THE USE THEREOF NOTICE TO PURCHASER LIMITED LICENSE The combination of the GeneAmp PCR System 9700 Sample Block Module with a GeneAmp PCR System 9700 Base Unit is licensed under the thermal cycler apparatus claims of US patents and patent claims in other countries No other rights are conveyed expressly by implication or by estoppel Further information on purchasing licenses may be obtained by contacting the Director of Licensing Aplied Biosystems 850 Lincoln Centre Drive Foster City California 94404 USA TRADEMARKS AB Design Applied Biosystems and MicroAmp are registered trademarks and Celera Genomics is a trademark of Applied Biosystems or its subsidia
16. If necessary press Cancel to exit the test To run the Calibration Verification Test Step Action 1 Complete the procedures in Configuring the System 9700 on page 1 23 2 Press Run This starts the Calibration Verification Test Note To press the menu items use the corresponding F keys For example Run is F1 on this menu The Calibration Verification screen appears with the setpoint value displayed Calibration Verification Block temp xx x C Cover temp xxx C Setpoint is 85 C Cover must be within 1 C of 105 C Fl F2 E3 F4 F5 Note The cover must be within 1 C of 105 C It may take several minutes for the system 9700 to ramp up 3 The Calibration Verification screen counts down the time until the setpoint is reached Calibration Verification Block temp xx x C Cover temp xxx C Stabilizing at setpoint x xx F1 F2 F3 FA F5 When the Stabilizing at setpoint value decrements to zero read the digital thermometer Note X Refer to the instructions included with your Temperature Verification Kit for a detailed description on operating the digital thermometer 1 24 0 5 mL Sample Block Module To run the Calibration Verification Test continued Step Action 4 Using the numeric keys type the value displayed on the digital thermometer in the Enter actual block temperature field Calibration Verification Block temp xx x C Cover temp xxx
17. Press Util The Utilities screen appears Note To press the menu items use the corresponding F keys For example Util is F4 on this menu Press Diag The Diagnostics screen appears Press System The System Performance screen appears System Performance Rate Cool and Heat Rate Test Cycle Cycle Performance Test Rate Cycle Press Cycle from the System Performance screen This runs the Cycle Test WARNING Install the appropriate empty Consumables into the Sample Block Refer to System Performance Section of Block User Manual F1 F2 F3 F4 F5 Note You will install the GeneAmp 0 5 mL Thermal Insulation Frame Place the 0 5 mL Thermal Insulation Frame in the sample block Slide the heated cover forward and pull the lever down After you have installed the frame press Cont The Cycle Test executes a two temperature PCR cycling protocol then measures and reports the average cycle time and the cycle to cycle variation IMPORTANT Pressing Pause during the Cycle Test may generate false test results Re run the Cycle Test if Pause was pressed during the test 1 36 0 5 mL Sample Block Module To run the Cycle Test continued Step Action 8 At the conclusion of the test the Cycle Performance screen appears The screen displays the test results and whether the test passed or failed Cycle Performance Pass Average Cycle Time xxx x sec Cycle Time
18. Use As Shown GeneAmp9 GeneAmp Thin WalledTubes O5 mL n SEDED j Thin Walled Tubes with Flat Caps Thermal with Flat Caps Insulation Frame GeneAmp 0 5 mL Thermal Insulation Frame Sample block Loading Samples To load samples into the reaction tubes into the Reaction Tubes Step Action 1 Pipette samples into the reaction tubes Note The sample volume range is 20 100 pL Close the reaction tubes with the flat caps Continue with Placing the Reaction Tubes into the Sample Block on page 1 18 0 5 mL Sample Block Module 1 17 Placing the Reaction To place the reaction tubes into the sample block Tubes into the Sample Block Step Action 1 Place the GeneAmp Thin Walled Tubes with Flat Caps directly into the sample block Set the GeneAmp 0 5 mL Thermal Insulation Frame over the tubes See the diagram below IMPORTANT Be sure to use the frame It insulates your samples and prevents the heated cover from damaging the reaction tubes 2 Slide the heated cover forward Note To ensure a proper seal pull the heated cover completely forward 3 Pull the heated cover lever down to engage the heated cover with the reaction tubes 4 Process your samples as usual 1 18 0 5 mL Sample Block Module Cleaning the Sample Block Module Cleaning Overview Cleaning Position Cleaning the Heated Cover The following procedures describ
19. ation Verification Test you will perform the following procedures Step Action See Page 1 Setting Up the 0 5 mL Probe Assembly 1 22 2 Configuring the System 9700 1 23 3 Running the Test 1 24 4 Evaluating the Results 1 26 This test requires the 0 5 mL Sample Block Module Temperature Verification Kit P N 4309924 Your kit includes Cotton swabs 9 9 9 Light mineral oil GeneAmp 0 5 mL Thermal Insulation Frame 0 5 mL Probe Assembly Digital thermometer with 9V battery installed 0 5 mL Sample Block Module 1 21 Setting Up the 0 5 mL Probe To set up the 0 5 mL Probe Assembly Assembly Step Action 1 If the heated cover is in the forward position lift the lever then slide the heated cover back Place the GeneAmp 0 5 mL Thermal Insulation Frame on the sample block Use a cotton swab to coat well A6 with mineral oil Place the 0 5 mL Probe Assembly into well A6 Q P NN Thread the probe wire through the channel in the 0 5 mL Thermal Insulation Frame to prevent damage to the probe and lead wires o Make sure the probe is connected to the digital thermometer Slide the heated cover forward and pull the lever down IMPORTANT Seat the probe properly and close the heated cover carefully If the probe wire is crushed when the heated cover is closed the probe may be damaged Turn on the digital thermometer Note Refer to the instructions i
20. e The cleaning position of the sample block module Howto clean the heated cover How to clean the sample block wells IMPORTANT Before using any cleaning or decontamination method except those recommended in this manual check with Applied Biosystems to ensure that the proposed method will not damage the equipment To clean the 0 5 mL Sample Block Module slide the heated cover back then slide the protrusions on the lid up through the vertical slots in the module rails The cleaning position is shown below Protrusion Protrusion Vertical slot Clean the heated cover once a month or more frequently if needed WARNING During instrument operation the temperature of the heated cover can be as high as 108 C and the temperature of the sample block module can be as high as 100 C Before performing this procedure wait until the heated cover and sample block module reach room temperature To clean the heated cover Step Action 1 Turn off the instrument 2 Wait 20 30 minutes for the heated cover to cool down 3 Raise the heated cover lever and slide the cover back almost but not completely to the back of its slide 4 Line up the protrusions on the side of the heated cover with the vertical slots in the module rails 5 Lift up the front of the heated cover until the protrusions travel up the vertical slots all the way to the top 6 Soak a cotton swab or piece of clean clo
21. e 1 29 Running the Test zoe ooh ce eta DO e EA Qe een d ree eu 1 30 Evaluating the Results san an a a a l AAA III 1 33 Running System Performance Diagnostics 1 34 OVEEVICW s vvv E T DANA A AA DARE A Malad a nk 1 34 Equipment Required uictor ecd e Ma AG 1 34 Running the Rate Test coste Revier eer e m bra e E ree 1 34 Running the Cycle Test i xeya 3 dae E LR S XA E RED HERE EE DU IN REP 1 36 Data Sheet Calibration Verification Test 1 38 Instt ctions DD Bebe hadi tend E REDE i 1 38 Data Sheet Temperature Non Uniformity Test 1 39 Instr ctions 22 e en okie sana ehh ei ae ita S ene 1 39 Index 0 5 mL Sample Block Module Safety and Regulatory Information IMPORTANT Read this section before you install the GeneAmp PCR System 9700 and the 0 5 mL Sample Block Module General Symbols The following symbols are used on Applied Biosystems instruments Whenever such symbols appear on instruments please observe appropriate safety procedures Electrical Symbols This symbol indicates the on position of the main power switch This symbol indicates the off position of the main power switch This symbol indicates the on off position of a push push main power switch This symbol indicates that a terminal may be connected to another instrument s signal ground reference This is not a
22. e 1 5 generaluse 1 3 grounding and electrical safety 1 4 input output connections 1 4 instrument storage 1 5 physical specifications 1 4 pollution categoy 1 5 routine maintenance 1 5 sample block module 1 4 voltage quality 1 4 ordering parts 1 13 Index 1 P parts modules accessories and disposables 1 13 ordering 1 13 PCR cycling function testing 1 35 1 36 Peltier unit heating cooling unit 1 12 verifying running correctly 1 33 performance tests 1 33 1 36 Cycle Test running 1 35 1 36 Rate Test running 1 33 1 34 pollution category 1 5 R Rate Test running 1 33 1 34 reaction tube configuration 1 16 loading samples 1 16 S safety and regulatory information 1 1 1 2 electrical safety testing 1 2 instrument labels 1 2 sample block about 1 11 cleaning the wells 1 19 modes 1 11 verifying calibration 1 20 1 25 0 5 mL Probe Assembly 1 21 evaluating the results 1 25 required equipment 1 20 running the test 1 23 1 25 verifying temperature uniformity 1 26 1 32 0 5 mL Probe Assembly 1 27 evaluating the results 1 32 required equipment 1 26 running the test 1 29 1 31 wells DNA contamination 1 19 sample block modules interchanging 1 10 sample control mode 1 11 samples loading 1 16 1 17 loading into reaction tubes 1 16 placing into the sample block 1 17 specifications physical 1 4 storing instrument 1 5 symbols burns warning 1 2 Index 2 Communautes Europeennes CE Ww Compliance 1 5
23. e empty Consumables into the Sample Block Refer to System Performance Section of Block User Manual F1 F2 F3 F4 F5 Note You will install the GeneAmp 0 5 mL Thermal Insulation Frame Place the 0 5 mL Thermal Insulation Frame in the sample block Slide the heated cover forward and pull the lever down After you have installed the frame press Cont The instrument runs through a series of tests where the sample block is stabilized at 35 C 94 C and 4 C 1 34 0 5 mL Sample Block Module To run the Rate Test continued Step Action 8 At the conclusion of the test the Cool and Heat Rate Test screen appears The screen displays the test results and whether the test passed or failed Cool and Heat Rate Test Heating rate x xx C s Cooling rate x xx C s F1 F2 F3 F4 F5 Check your Rate Test results against the passing ranges listed below Heating Rate gt 1 5 C second Cooling Rate gt 1 5 C second If the test fails repeat the procedure once If the test fails again contact Applied Biosystems Technical Support See Technical Support on page 1 6 0 5 mL Sample Block Module 1 35 Running the Cycle Use the Cycle Test to verify that the PCR cycling function operates properly This test Test takes approximately 15 minutes to run To run the Cycle Test Step Action 1 Turn on the system 9700 The main menu appears 2
24. eni om biond ne dace aba ee EE eed ee 1 6 Hours for Telephone Technical Support 0 0 0 cee KK KK KK KK KK KK KK KK 1 6 To Reach Us by Telephone or Fax in North America 1 6 Document orn Demand lt s44 a s kul ek c WW bl El W bu A 1 7 To Reach Us by E Mail cessus gp pg RE ee ek gp 1 8 Regional Offices Sales and Service eee eee 1 8 About the 0 5 mL Sample Block Module WWW KK KK KK KK KK KK KK KK KK eee 1 11 OV6erVIEW dos DN tet e CC etos pre t A gt 335 ZM U n 1 11 Da SPAM nn oe er CREE e CHE Rate e eld em REGN NERA wale kere WEK 1 11 Sample Block qa Sea a yay a E NE ie biota oe eae ae be ERO E RISUS 1 12 Heated Covers Avice ERR eo Sa ue am eu Mi IM iui zu 2 2 ommgm 1 13 Peltier Heating Cooling Unit KK KK KK KK KK KK eee 1 13 Modules Accessories and Disposables 1 14 OVerVIeWat tes nada adala sae nae eb ates seit ucc oris um 1 14 Ordernmng ciu 5 xwana ur ARO KEY d ede ume id Sei le Mend edes 1 14 Installing the 0 5 mL Sample Block Module n on WW KK KK KK KK KK KK KK ee eee 1 15 OVerVIEW Lenoir MEREPIERRPGS ph pm 1 15 Installing the Sample Block Module KK KK enn 1 16 Loading Samples At nuthin Wa Da aati ines AN AAA E oad adaa 1 17 Loading Samples Overview kk kK kK KK KK KK KK KK KK KK KK KK eh 1 17 Reaction Tube
25. erse effects on the electronic components of the instrument In areas where the supplied power is subject to fluctuations exceeding these limits a power line regulator may be required Refer to the GeneAmp PCR System 9700 Base Module User s Manual P N 0993 6247 for information on the input and output connections of this instrument Refer to the GeneAmp PCR System 9700 Base Module User s Manual P N 0993 6247 for information on the physical specifications of this instrument 1 4 0 5 mL Sample Block Module Pollution Category Instrument Storage Communautes Europeennes CE Compliance FCC Compliance U S Routine Maintenance for Safe Operation The system 9700 will operate safely in environments that contain nonconductive foreign matter up to Pollution Degree 2 in IEC 1010 1 The system 9700 must be stored at a temperature between 20 C and 60 C 4 F and 140 F at altitudes ranging from 0 12 000 meters above sea level IMPORTANT The system 9700 is guaranteed to meet performance specifications only when the ambient temperature is 15 30 C and the ambient relative humidity is 20 80 between altitudes of 0 and 2 000 meters European Community have the CE label on the back of the instrument This label signifies that these instruments comply with the Electromagnetic C All instruments shipped to the European Union EU formerly known as the Compatibility and Low Voltage Directives Th
26. h another sample block module as needed Interchangeability lets you change sample well formats as well as throughput capacity IMPORTANT Interchanging sample block modules may require a firmware change If the sample block module is placed on a base module running an earlier software version the instrument will not operate correctly To upgrade the base module software use the 3 5 floppy disk that is shipped with the 0 5 mL Sample Block Module For instructions on upgrading the software see the GeneAmp PCR System 9700 Base Module User s Manual P N 0993 6247 or visit our web site www appliedbiosystems com techsupport 0 5 mL Sample Block Module Heated cover System 9700 Sample block Base Module 0 5 mL Sample Block Module 1 11 Sample Block The sample block has 60 wells 10 x 6 for use with GeneAmp Thin Walled Tubes with Flat Caps 0 5 mL volume The sample block can operate in one of two modes Sample control 0 5 mL Mode button In this mode the instrument calculates the temperature of the sample during cycling You can enter various reaction volumes and the instrument will compensate for this e g 20 uL heats and cools faster than 100 uL In this mode the sample block itself overshoots the setpoint temperature to get the sample to temperature more quickly but the sample temperature will not overshoot The clock starts counting down hold time once the sample is within one degree of the setpoin
27. is product is classified as a digital device used exclusively as industrial commercial or medical test equipment It is exempt from the technical standards specified in Part 15 of the FCC Rules and Regulations based on Section 15 103 c Before using any cleaning or decontamination method except those recommended in the manual the user should check with Applied Biosystems to ensure that the proposed method will not damage the equipment Maintain your instrument in good working order In the event that the instrument has been subjected to adverse environmental conditions such as fire flood earthquake etc a service inspection of the instrument should be made to ensure safe operation 0 5 mL Sample Block Module 1 5 Technical Support To Reach Us on the Applied Biosystems web site address is Web http www appliedbiosystems com techsupport We strongly encourage you to visit our web site for answers to frequently asked questions and to learn more about our products You can also order technical documents and or an index of available documents and have them faxed or e mailed to you through our site see the Documents on Demand section below Hours for Telephone In the United States and Canada technical support is available at the following times Technical Support Product Hours Chemiluminescence 9 00 a m to 5 00 p m Eastern Time LC MS 9 00 a m to 5 00 p m Pacific Time All Other Products 5 30 a m to 5 00 p
28. l support please call our local office or refer to our Web site at www appliedbiosystems com Applied Biosystems is committed to providing the world s leading technology and information for life scientists Headquarters 850 Lincoln Centre Drive Foster City CA 94404 USA Phone 1 650 638 5800 Toll Free In North America 1 800 345 5224 Fax 1 650 638 5884 09 2010 amp Applied E Biosystems Part Number 4307808 Rev D
29. m Pacific Time See the Regional Offices Sales and Service section below for how to contact local service representatives outside of the United States and Canada To Reach Us by Call Technical Support at 1 800 831 6844 and select the appropriate option below Telephone or Fax in for support on the product of your choice at any time during the call To open a North America Service call for other support needs or in case of an emergency press 1 after dialing 1 800 831 6844 For Support On This Product Dial 1 800 831 6844 and amp ABI PRISM 3700 DNA Analyzer Press FAX 8 650 638 5891 a ABI PRISM 3100 Genetic Analyzer Press FAX 26 650 638 5891 Biolnformatics includes BioLIMS BioMerge and SQL GT applications 5 505 982 7690 DNA Synthesis Press FAX 21 650 638 5981 Fluorescent DNA Sequencing Press FAX 22 650 638 5891 Enere stellt Fragment Analysis includes Press FAX GeneScan applications 23 650 638 5891 Integrated Thermal Cyclers Press FAX 24 650 638 5891 1 6 0 5 mL Sample Block Module For Support On This Product Dial 1 800 831 6844 and PCR and Sequence Detection Press FAX 5 or call 1 800 762 4001 and press 1 for PCR or 2 for Sequence Detection 240 453 4613 FMAT Telephone FAX 1 800 899 5858 and press 1 then press 6
30. mple Block Module Lever 2 d Up I n I I 69 le C I E J Bottom view 0 5 mL Sample Block Module 0 5 mL Sample Block Module 1 15 Installing the To install the sample block module into the GeneAmp PCR System 9700 Base Sample Block Module Module Step Action 1 Pull the lever out from the sample block module 2 Place the sample block module onto the base module then push the sample block module back to seat the electrical connections 3 Push the lever into the base module to secure the sample block module Note Ifthe sample block module is not seated correctly the instrument cannot be turned on 1 16 0 5 mL Sample Block Module Loading Samples Loading Samples Overview Reaction Tube Frame Configuration The following procedures describe The reaction tube frame configuration How to load samples into the reaction tubes How to place the reaction tubes and frame into the sample block Use the following reaction tube frame configuration for the 0 5 mL Sample Block Module With this vessel
31. n the Enter actual block temperature field TNU Performance Sample temp xx x C Cover temp xxx C Enter actual block temperature 00 0 F1 F2 F3 F4 F5 Note The digital thermometer displays a four digit value round this off to three digits before typing it in the TNU Performance screen Note If desired record this value on the Temperature Non Uniformity Test Data Sheet page 1 39 to keep a permanent record of the test Press ENTER The system 9700 automatically begins the second reading 37 C setpoint The TNU Performance screen appears with the setpoint value displayed TNU Performance Sample temp xx x C Cover temp xxx C Setpoint is 37 C Sample must be within 1 0 C of setpoint F1 E2 F3 F4 F5 Note The sample block must be within 1 0 C of the setpoint Repeat steps 3 and 4 for the second reading Press ENTER The TNU Performance screen appears with the following prompt TNU Performance Sample temp xx x C Cover temp xxx C Place probe in well xx Press Run Run 0 5 mL Sample Block Module 1 31 To run the Temperature Non Uniformity Test continued Step Action 8 Slide the heated cover back and repeat steps 4 7 of Setting Up the 0 5 mL Probe Assembly on page 1 28 and steps 2 7 of this procedure Complete these steps for all 12 wells to be tested A1 D1 A6 D7 A10 E8 B3 F1 C5 F5 C10 F10
32. ncluded with your Temperature Verification Kit for a detailed description on operating the digital thermometer Continue with Configuring the System 9700 on page 1 23 1 22 0 5 mL Sample Block Module Configuring the To configure the system 9700 for the Calibration Verification Test System 9700 Complete the procedures in Setting Up the 0 5 mL Probe Assembly on page 1 22 Turn on the system 9700 The main menu appears Press Util The Utilities screen appears Note To press the menu items use the corresponding F keys For example Util is F4 on this menu Press Diag The Diagnostics screen appears Press TmpVer The Temperature Verification screen appears Temperature Verification Temp Calibration Verification NU Temperature Non Uniformity Temp J TNU F1 F2 F3 F4 F5 Press Temp This automatically configures the system 9700 for the Calibration Verification Test The Calibration Verification screen appears Calibration Verification lock temp xx x C Cover temp xxx C Place probe in well A6 Press Run Run Cancel Fl F2 F3 FA F5 Continue with Running the Test on page 1 24 0 5 mL Sample Block Module 1 23 Running the Test Use the digital thermometer to take temperature readings of the sample well connected to the 0 5 mL Probe Assembly You will take a reading at two different setpoint temperatures Note
33. not designed for operation in an explosive environment Do not place the instrument close to potentially explosive materials or objects CAUTION This instrument is not designed for operation with the heated cover retracted when running at 4 C If the heated cover is retracted and the instrument runs at 4 C water condensation may be excessive in the sample block area PP PP 0 5 mL Sample Block Module 1 3 Grounding and Electrical Safety Voltage Quality Input Output Connections Physical Specifications Sample Block Module WARNING To protect yourself against burns do not open the heated cover or touch the sample block module when the word Hot displays on the screen This indicates a sample block temperature above 50 C The hot areas for the 0 5 mL Sample Block Module are shown below CAUTION To protect your samples and to guarantee the best except when loading or unloading samples temperature uniformity keep the heated cover closed at all times The system 9700 must be grounded for protection against electrical shock WARNING Do notuse an adapter to a two terminal outlet since 5 this does not provide positive ground protection Refer to the GeneAmp PCR System 9700 Base Module User s Manual P N 0993 6247 for more information on electrical safety Line voltage must be within 10 of the nominal value High or low voltages may have adv
34. nternational access code then 1 858 712 0317 froma Have your complete fax number and country code ready 011 precedes the country code b Press 1 to order an index of available documents and have it faxed to you Each document in the index has an ID number Use this as your order number in step d below c Call 1 858 712 0317 from a touch tone phone a second time d Press 2 to order up to five documents and have them faxed to you To Reach Us by Contact technical support by e mail for help in the following product areas E Mail Regional Offices For this product area Use this e mail address Chemiluminescence info appliedbiosystems com Genetic Analysis galab appliedbiosystems com LC MS apisupport sciex com PCR and Sequence Detection pcrlab appliedbiosystems com Protein Sequencing Peptide and DNA Synthesis corelab appliedbiosystems com If you are outside the United States and Canada you should contact your local Sales and Service Applied Biosystems service representative The Americas United States Applied Biosystems 850 Lincoln Centre Drive Foster City California 94404 Tel 650 570 6667 800 345 5224 Fax 650 572 2743 Latin America Del A Obregon Mexico Tel 305 670 4350 Fax 305 670 4349 Europe Austria Wien Hungary Budapest Tel 43 0 1 867 35 750 Tel 36 0 1 270 8398 Fax 43 0 1 867 35 75 11 Fax 36 0 1
35. osed the probe may be damaged Turn on the digital thermometer Note Refer to the instructions included with your Temperature Verification Kit for a detailed description on operating the digital thermometer Continue with Configuring the System 9700 on page 1 29 1 28 0 5 mL Sample Block Module Configuring the To configure the system 9700 for the Temperature Non Uniformity Test System 9700 Complete the procedures in Setting Up the 0 5 mL Probe Assembly on page 1 28 Turn on the system 9700 The main menu appears Press Util The Utilities screen appears Note To press the menu items use the corresponding F keys For example Util is F4 on this menu Press Diag The Diagnostics screen appears Press TmpVer The Temperature Verification screen appears Temperature Verification Temp Calibration Verification NU Temperature Non Uniformity Temp J TNU F1 F2 F3 F4 F5 Press TNU This automatically configures the system 9700 for the Temperature Non Uniformity Test The TNU Performance screen appears TNU Performance Sample temp xx x C Cover temp xxx C Place Probe in well Al Press Run Run F1 F2 F3 F4 F5 Continue with Running the Test on page 1 30 0 5 mL Sample Block Module 1 29 Running the Test The Temperature Non Uniformity Test uses the 0 5 mL Probe Assembly to test the temperature uniformity of 1
36. protected ground terminal This symbol indicates that this is a protected ground terminal that must be connected to earth ground before any other electrical connections are made to the instrument This symbol indicates that this terminal either receives or delivers alternating current or voltage This symbol indicates that this terminal either receives or delivers alternating and direct current or voltage A A A O 0 5 mL Sample Block Module 1 1 This symbol indicates the presence of high voltage and warns the user to proceed with caution This symbol alerts you to consult the manual for further information and to proceed with caution Non electrical Symbols This symbol illustrates a heater hazard Proceed with caution when working around these areas to avoid being burned by hot components gt Electrical Safety Routine safety testing of analytical instruments e g high potential voltage testing Testing may be required by various safety agencies Testing should only be carried out by qualified personnel after seeking advice from the Applied Biosystems Service Department gt Instrument Labels The following instrument labels are on the system 9700 with the 0 5 mL Sample Block Module installed WARNING Disconnect supply cord before opening Grounding circuit continuity is vital for safe operation of equipment Never operate equipment
37. ries in the U S and or certain other countries GeneAmp is a registered trademark of Roche Molecular Systems Inc All other trademarks are the sole property of their respective owners Part Number 4307808 Rev D 09 2010 Contents 1 0 5 mL Sample Block Module Safety and Regulatory Information ee 1 1 General Symbols esere xaka 0ya kn eme eb nma eR et MENS DER ER REV ER RENE 4 3 1 1 Electrical Safety Testings lt i 2k Un WNN WE E e e K e 1 2 Instrument Labels 5 ece eee epe ote erri P EE VERO FECE 1 2 Operating Precautions isses get e e Rp ber AN A SA RE TES 1 3 Precautions soe eee aO hp ede ee A reU S ee e e exu eis 1 3 Grounding and Electrical Safety KK KK KK KK KI 1 4 Noltage Quality SS Dan D ees BA ERR URS Ca Aah Ok AG aa A RN Ai 1 4 Input Output Connections 0 0 0 0 kK KK KK KK KK KK KK KK KK KK kk KK kK KK ene 1 4 Physical Specifications gt gt s cies XW E ae S V Re neu d BEWARE TNE ASA 1 4 Pollution Cat gorya lt 55 2 Leh SANA ee Se a eg 1 5 Instrument Storage elei Ses eke ld Esl arin RICE Neo PerRE w n k ya vana J 1 5 Communautes Europeennes CE Compliance 1 5 FCC Compliance U SJA e eher erret S m eedem 1 5 Routine Maintenance for Safe Operation 1 5 Technical Support us zs t aee ee ig a eee OX RE ER REG E ba SA Sa ae 1 6 To Reach Us On the Web S
38. t temperature Note The temperature displayed on the screen is the calculated sample temperature The illustration below depicts the profile of the sample control mode 0 5ml Sample Block Block Temperature vs Sample Temperature Sample Temperature 77 Block Temperature Temperature C 40 30 20 10 0 10 20 30 40 50 60 70 80 90 100 110 120 T ime fa Block control Block Mode button In this mode the reaction volume and sample temperature are ignored The sample block itself goes to the programmed setpoint without overshooting The clock starts counting down hold time once the sample block reaches the setpoint temperature Note The temperature displayed on the screen is the block temperature 1 12 0 5 mL Sample Block Module Heated Cover The heated cover slides over the sample block and performs two functions It raises the temperature of the upper part of the tube the part above the sample block above the temperature of the sample mixture This eliminates condensation which may have a negative effect on your chemistry lt puts pressure on the reaction tubes and frame to seat them firmly and precisely in the sample block This is important for proper heat transfer Peltier Heating The internal Peltier heating cooling unit is housed in the sample block module The Cooling Unit sample block module is made of aluminum to provide the optimal thermal transfer rate A Resis
39. th with pure isopropanol and gently wipe the bottom of the cover 7 Remove any remaining isopropanol from the cover and return the cover to its normal position 0 5 mL Sample Block Module 1 19 To clean the heated cover continued Step Action 8 If the cover becomes contaminated with amplified DNA a Raise the heated cover to the cleaning position b Wipe the cover with a cloth or cotton swab soaked in 10 bleach c Wipe with a damp cloth Cleaning the Sample Clean the sample block wells once a month or more frequently if needed Block Wells To clean the sample block wells Step Action 1 Turn off the instrument 2 Wait 1 minute for the sample block module to cool 3 Remove the reaction tubes and frame from the sample block module and set them aside Use a cotton swab soaked in pure isopropanol to clean the sample block wells thoroughly Remove any remaining isopropanol from the heated cover before reloading the reaction tubes and frame If the sample block wells become contaminated with amplified DNA a Clean the wells thoroughly with a cotton swab soaked in 10 bleach b Wipe with a damp cloth 1 20 0 5 mL Sample Block Module Running the Calibration Verification Test Overview Use this test to verify the temperature calibration of your GeneAmp PCR System 9700 0 5 mL Sample Block Module Equipment Required To complete the Calibr
40. tive Temperature Device RTD sensor in the sample block module provides Wide temperature range 4 99 9 C Accuracy 0 25 C from 35 100 C Heat cool rate 1 5 C per second Temperature uniformity 0 5 C measured 30 seconds after the clock starts 9 9 9 Long term stability and high reliability 0 5 mL Sample Block Module 1 13 Modules Accessories and Disposables Overview The modules and accessories listed below are available for the GeneAmp PCR System 9700 Base Module The disposables are for use with the 0 5 mL Sample Block Module Ordering Order modules accessories and disposables from Applied Biosystems by part number See the tables below Modules and Accessories Part Number 96 Well Sample Block Module N805 0251 Dual 384 Well Sample Block Module N805 0400 0 5 mL Sample Block Module 4309131 0 5 mL Sample Block Module Temperature Verification Kit 4309924 Disposables Part Number GeneAmp 0 5 mL Thermal Insulation Frame box of 8 4308927 GeneAmp Thin Walled Tubes with Flat Caps N801 0737 1 14 0 5 mL Sample Block Module Installing the 0 5 mL Sample Block Module Overview A lever behind the sample block module releases it from the GeneAmp PCR System 9700 Base Module Diagrams of the top and bottom of the 0 5 mL Sample Block Module are shown below GR1402 Top view 0 5 mL Sa
41. u will perform the following procedures Step Action See Page 1 Setting Up the 0 5 mL Probe Assembly 1 28 2 Running the Test 1 30 3 Evaluating the Results 1 33 This test requires the 0 5 mL Sample Block Module Temperature Verification Kit P N 4309924 Your kit includes Cotton swabs 9 9 9 Light mineral oil GeneAmp 0 5 mL Thermal Insulation Frame 0 5 mL Probe Assembly Digital thermometer with 9V battery installed 0 5 mL Sample Block Module 1 27 Setting Up the 0 5 mL Probe To set up the 0 5 mL Probe Assembly Assembly Step Action 1 If the heated cover is in the forward position lift the lever then slide the heated cover back Place the GeneAmp 0 5 mL Thermal Insulation Frame on the sample block Use a cotton swab to coat the following wells with mineral oil A1 D1 A6 D7 A10 E8 B3 F1 C5 F5 C10 F10 Place the 0 5 mL Probe Assembly into well A1 Note As the test progresses you will move the 0 5 mL Probe Assembly to each of the test wells Thread the probe wire through the channel in the 0 5 mL Thermal Insulation Frame to prevent damage to the probe and lead wires Make sure the probe is connected to the digital thermometer Slide the heated cover forward and pull the lever down IMPORTANT Seat the probe properly and close the heated cover carefully If the probe wire is crushed when the heated cover is cl
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