DRS-36 User Manual
Contents
1. Data acquisition system that a DRS 36 is installed into Input Board 32 channel 24 bit data acquisition board that fits in a Digital Lynx slot alongside other Input Boards or DRS 36s Reference Signal The signal that is subtracted from the desired electrode signal to reduce the amplitude of noise artifact and other signals that are part of the desired electrode signal The reference electrode is usually placed in a quiet part of the brain or a ground screw in the skull CMRR Common Mode Rejection Ratio is a measurement based on the amount of residual signal left error after the reference signal is subtracted from the desired electrode signal This is a ratio measurement and usually measured in decibels or as the amount of reduction of the common mode signal on both the input and reference A measurement ratio of 10 000 1 is also 80db Decibels db A logarithmic measurement of two signals with the function 20 log10 V1 V2 Global Reference A reference signal that is shared between all DRS 36 boards Global Reference Bus GRB 8 Global Reference signals shared between the DRS 36 boards Global Reference Bus Cable The cable that connects the Global Reference signals between the DRS 36 boards Local Reference A signal on the DRS 36 board which is used as a reference signal output This signal may come from a headstage signal or a GRB input Local Reference Bus LRB The bus of 8 signals which can be used as t2. Phone 406 585 4542 lt Fax 406 585 9034 www Neuralynx com Revision support Neuralynx com 10 27 2010 Page 21 11 Switching Options The DRS 36 has the following Signal Switching functions e Any of the 37 Headstage Signals may be used as any of the 8 LRB Signals e Any of the 8 external GRB Inputs may be used as the LRB signals The GRB signals have a restriction in that each GRB signal may be used as the respective corresponding LRB signal For example GRB3 may only be used as LRB3 e Any individual LRB signal may be driven by either a headstage signal or by a GRB signal but not by both e Any LRB signal originating from the Headstage may be driven out to the GRB connector and used by other boards through their GRB connector e Each of the 32 Output Channels reference signal may be selected from any of the 8 LRB signals These rules have been implemented in the Cheetah DRS 36 User Interface Control Panel Neuralynx Inc 105 Commercial Drive Bozeman MT 59715 Phone 406 585 4542 lt Fax 406 585 9034 www Neuralynx com Revision support Neuralynx com 10 27 2010 Page 22 12 User Interface in Cheetah The Cheetah Software version 4 95 20070112 or later must be used with the DRS 36 This version has a new window with the DRS 36 controls Current versions of the Cheetah Software 5 0 0 and higher include a complete Cheetah Reference Guide accessible through the Help menu which fully document the DRS user
3. This document is intended for all users of the Neuralynx Digital Lynx data acquisition system 3 DRS 36 Overview The DRS 36 offers flexibility and precise control of neural electrode input reference signal selection This includes the ability to manage and control a pool of reference electrodes which ideally contains only error noise and artifact signals Each channel of a data acquisition system consists of a signal and reference inputs The Digital Lynx Input Board performs the signal minus reference subtraction function in hardware The Neuralynx Inc 105 Commercial Drive Bozeman MT 59715 Phone 406 585 4542 lt Fax 406 585 9034 www Neuralynx com Revision support Neuralynx com 10 27 2010 Page 3 DRS 36 allows the user to use any electrode signal as a reference for any other Digital Lynx Input Board input channel The DRS 36 supports one HS 36 headstage pre amplifier 8 reference signals connection to a Digital Lynx Input Board 32 differential channels and 8 Global Reference signals which can be shared between multiple DRS 36 boards in a single Digital Lynx system Neuralynx Inc 105 Commercial Drive Bozeman MT 59715 Phone 406 585 4542 lt Fax 406 585 9034 www Neuralynx com Revision support Neuralynx com 10 27 2010 Page 4 4 Glossary The following terms will be used throughout this document DRS 36 Digital Reference Selection for 36 channel headstage pre amplifiers Digital Lynx
4. Page 2 Table of Figures Figure 5 1 Block Diagram of DRS sees 8 Figure 5 2 Plead Stas Mi Ub scares nctosceeatesacecien saecen genset ndueecencio AEE E RRRS 9 Figure 5 3 To BSB TT 10 Fig re 6 1 DRS Board aasre ca eeteted teeth thaeed iat ets tisine ideii aniis TREO 12 Fig re 7 1 Headst g POU series ctren 2a do ucsectieecdesantaecoes tee doeeceanedeeadacceateuedsavtaeeeeeancemczenceeee 14 Fig te 7 2 LRF CONDECION oien ir nee epee an Nt oP ater A teresa te 15 Figure 7 3 80 Pin Differential Oum ss sss sese es sees eee ee eee eee 17 Figure 7 4 Moritor CUO ars cic dg odene ets GelenteS elas et ve cea nese dale me aiea seni ns ainin 18 Figure 8 1 Global Reference Cable caved eee 19 Figure 8 2 Global Reference Cable Pinout sss sese eee ee eee inne 20 Pipure 14 1 Blue Jumper Placement usisna 25 Figure 14 2 Dip Switch Location sss sees ee sese eee sadecesd ncbacadaiatedeeaes 26 1 Document Revision History 10 19 2010 Rev 1 2 A section has been included to make sure a secure connection is made with the GRB Cable 6 2 2 2 Document Overview This document will describe Digital Reference Selection for 36 channel headstage pre amplifiers DRS 36 hardware features and installation It will also cover concepts and strategies for effective DRS 36 use including how Cheetah software is used to control the board and other advanced software features The Global Reference Bus Cable and other miscellaneous features of the board are also covered
5. and LFP recording setups The exception would be for EMG differential signals which require one reference for each input channel For a 32 channel EMG differential recording setup the 64 signals would Neuralynx Inc 105 Commercial Drive Bozeman MT 59715 Phone 406 585 4542 lt Fax 406 585 9034 www Neuralynx com Revision support Neuralynx com 10 27 2010 Page 8 be connected directly through the headstages to the Input Boards A DRS 36 or ERP would not normally be used for this recording setup The sources for these 8 reference signals include the above mentioned headstage signal Panel Ground and any of 8 Global Reference Bus signals which allow sharing of references between DRS 36 boards These 8 reference signals will be referred to as the Local Reference Bus signals 6 2 2 Global Reference Bus GRB The Global Reference Bus GRB is a set of eight signals connected between DRS boards to allow sharing of reference signals among boards These signals are connected to the front panel 3M MDR 26 connector A Global Reference Bus Cable is used to connect these signals in parallel between multiple DRS boards in a system If using the first version of the GRB Black Connectors please make sure to securely fasten the connection using the screws on each connector If using the second version of the GRB Silver Connectors please make sure the side clips clamp for a secure connection The Global Reference Bus signals are
6. interface 13 Configuration File Commands The DRS settings are saved in configuration files for use in future Cheetah recording sessions The commands and syntax are documented in the Cheetah Reference Guide accessible through the Help menu in Cheetah DRS Commands are found under Cheetah Commands in the Cheetah Reference Guide index 14 DRS 36 Serial ASCII commands The serial ASCII command strings can be used to control the DRS 36 with Cheetah configuration or NetCom commands If this is done the changes will not be reflected in the User Interface This operation is not recommended Contact Neuralynx Technical Support if the low level Serial ASCII strings are needed For Technical Support email support neuralynx com Neuralynx Inc 105 Commercial Drive Bozeman MT 59715 Phone 406 585 4542 lt Fax 406 585 9034 www Neuralynx com Revision support Neuralynx com 10 27 2010 Page 23 15 Installation The following steps are required to install and use the DRS 36 in the Digital Lynx system with the Cheetah Software 1 Place the DRS 36 and Input Boards in the Digital Lynx cabinet This will usually consist of an Input Board followed by a DRS 36 board for the number of Input Boards in your system For example if you have 128 channels on 4 Input Boards your cabinet will have e Slot 0 Input Board Slot 1 DRS 36 Slot 2 Input Board Slot 3 DRS 36 Slot 4 Input Board Slot 5 DRS 36 Slot 6 Input Board Slot 7 DRS 36 S
7. 0 Front Panel Lis cecer i e a E E ER 11 T Board and 1 doe en cca trcesisaee a uepceetics tesa EEEE E E EEE R 12 O Connectors oeae eerie OEE a EE AS EEE Oa E 13 Gel Headstage 5 611 issis iiris sence e aina aR EEEE EE EAER 13 8 2 Global Reference Bus Connectors sese eee eee 15 8 3 Differential Output Connector sss sees sees eee eee eee 16 8 4 Internal Single Ended Monitor Connector sss sees sese eee eee 18 Global B s Cable pits csicacdscsnsniassnietenieapsasuontansan aosonsdedsaeednesiauand a aeo aian Eaa iSe 19 OL Headstage Modifications sisscicnenansnn aa a 20 9 2 Communications Path from Cheetah sss esse sees eee nies 21 10 Board Numbering and Input Board A D Numbers sss sss sees 21 11 Switching Ops ediccgisencecds ectecktscdedet en cosedecicadd edkeesdiideadsainiescetaaceesiateadiaieieeed 22 12 User Interface in Cheetah sss sse meee namenemares 23 13 Configuration Fle Omnia sce sices covscsdasccstlseteanevauens ces bs aeendacensnceeeaeenteusbiaceey 23 14 DRS 36 Serial ASCH commands sss sese eee ee eee eee eee 23 15 r Er Hn casncsincassasnaasesubeswsmacssngnsdace chdeneanticslbsitenthseexaacemopedastira E E NEE OEE EE 24 16 10X differential Amp Section and Internal Monitor Output Connector 27 17 INA10X Ga in Sect sit risoria wee ase eaten 27 Neuralynx Inc 105 Commercial Drive Bozeman MT 59715 Phone 406 585 4542 lt Fax 406 585 9034 www Neuralynx com Revision support Neuralynx com 10 27 2010
8. 15 1 Blue Jumper Placement Neuralynx Inc 105 Commercial Drive Bozeman MT 59715 Phone 406 585 4542 lt Fax 406 585 9034 www Neuralynx com Revision support Neuralynx com 10 27 2010 Page 25 Using Table 2 below set four of the switches on the red DIP switch in the rear of the Digital Lynx Interface Box This red DIP switch is designated Config DIP Switch in the top image of Figure 15 1 on the previous page Nesign ON DIE W2 slon PR 2 2 2 p R 4 5 6 2 r HS TTT Figure 15 2 Dip Switch Location Note that the switch positions from left to right are 1 2 3 4 5 6 7 and 8 To set the number of boards we will use only switches 1 2 3 and 4 Use the following table to set the position of each of the 4 switches Use ONLY switches 1 through 4 Table 2 Red DIP Switch Settings I for Input Board D for DRS 36 Board I Up Up Up Up Up Up Up Up ID Up Up Up Up Up Up Up Up IDID Up Up Down Up Up Up Up Up IDx3 Up Down Up Up Up Up Up Up IDx4 Up Down Down Up Up Up Up Up IDx5 Down Up Up Up Up Up Up Up II Up Up Up Down Up Up Up Up Ix4 Up Up Down Down Up Up Up Up Ix5 Up Down Up Up Up Up Up Up Ix6 Up Down Up Down Up Up Up Up Ix7 Up Down Down Up Up Up Up Up Neuralynx Inc 105 Commercial Drive Bozeman MT 59715 Phone 406 585 4542 lt Fax 406 585 9034 www Neuralynx com Revision support Neuralynx com 10 27 2010 Page 26 Ix8 Up Down Down Down Up Up Up Up Ix9 Down Up Up Up Up Up Up Up I
9. 25 HSin28 48 20 HSIn24 HSRef4 44 ha n 19 S2 Ret PGnd_ Tether Shield 43 43 18 18 2 Sre HSin23 d Be F HSI 16 HSmMm0O Hsinz2 C oe MET HSin10 40 40 MAR HSIn9 eee 28 1 cans 5 HSIn18 37 38 IS HSIn6 HSIn17 36 27 12 71 HSIn5 HSIn16 35 36 11 Mio HSIn4 Gnd Ref 34 z K 9 HSRef3 HS Power Pos 33 33 8 8 S1 Ret HS Power Neg 32 32 7 7 1_Sre eds 44 ee HSin13 os 4 HSIn2 HS 28 2 JE HSin1 HSRefl 27 3 S L HSin0 HS Power Neg 26 1 HS Power Pos aie d AGnd Si B Figure 8 1 Headstage Pinout Neuralynx Inc 105 Commercial Drive Bozeman MT 59715 Phone 406 585 4542 lt Fax 406 585 9034 www Neuralynx com Revision support Neuralynx com 10 27 2010 Page 14 8 2 Global Reference Bus Connector The Global Reference Bus connector is a 3M 26 pin MDR connector a smaller version of the Headstage Input connector This connector has the 8 Global Reference Signals 8 ground connections Headstage 5v power switched on and off with the headstage power and external connections for the two differential Stimulus Channels 4 wires total The Stimulus Channels are present on this connector to allow connections from Stimulus Isolation Units to stimulus electrodes connected to the EIB or power signals for lighting video tracker LEDs mounted to the headstage The operation of the Stimulus Channel connections is the same as with all of the Neuralynx ERP xx patch panels which is that there are no electronic connections
10. Mode Rejection Ratio measurement is 80db to 77db 10 000 1 to 7 000 1 which is still sufficient for all standard electrophysiology experiment recording The exception would be in environments with very large signal artifacts such as an MRI signal artifact or other huge external signal Neuralynx Inc 105 Commercial Drive Bozeman MT 59715 Phone 406 585 4542 lt Fax 406 585 9034 www Neuralynx com Revision support Neuralynx com 10 27 2010 Page 6 6 Hardware Overview 6 1 Headstage Input Signals There are 37 headstage input signals These are e 32 input channels e 4 References Refl gt Ref4 e Animal Ground which is an unbuffered non current carrying wire used to connect the headstage ground to the DRS 36 or ERP xx for a reference signal Note that the DRS 36 will accept connections from the standard Neuralynx Headstage product line HS 36 HS 27x HS 18 HS 16 but not all signals and reference signals are present on all headstages 6 1 1 Headstage Power The headstage power supply outputs 5v and 5v There is no current limit but the solid state switches will start to limit current around 200 milliamps in theory Both power supplies are controlled by a set of 3 solid state PhotoMOS devices in a circuit that will turn on both power supply rails within a millisecond of each other The circuit is designed to minimize the chance of single supply turn on which is bad for the opamps and leakage currents on the headstag
11. an MT 59715 Phone 406 585 4542 lt Fax 406 585 9034 www Neuralynx com Revision support Neuralynx com 10 27 2010 Page 19 This is the DR5 36_Global_Bus_Cable schematic Casey 12 18 2006 Note Each MDR 26 has it s own dedicated 10 pin connector Keep the wire to the 10 pin connectors as short as possible 1 1 Src 1 51 Ret 2 91 Src 1 gt 2 51 Ret 1 2 Sre 1 2 Ret 2 92 Src 3 i 2 92 Ret D4 fe 5 st 4 6 G 7 R k Y 1 HS Pwr Pos 0 1 HS Pwr Neg 5v 2 HS Pwr Pos 5 9 2 HS Pwr Neg 5 2 HEADER 5X2 HEADER 5X2 3 These 9 wires are bundled together in the Bus Cable These 9 wires are bundled together in the Bus Cable E 3 i s a i AS 19 S S gis ie 2 lt 8 SISIR Z ESS SI S PEGEN A PEGER a EPFREE MEE E EPFREEME p HE gt gt 25 2 3 2 2 g B 92 25 2 3 2 ga Ga EGER iaa a iaka Z Beis SFO ea S 4 8 C B S B 8 o 8 8 S E d SATI STO a S S lt G a 5 v8 EE 5 SIS ol eeso eheloalolalie l al So welelslclaclel al g Slaa 2 Sa a a o D E Sial 22 2 D D 2 S D AE aiaa 3 SPIO aaaea G 8 4 BESS EI ol Soe SEEE aag o 2 74 38383888 oo SSSss va f Ka K a t C la Raa K kaa a GO F 3 HHHH BIH L P1 P2 SOO LOO wt CO 5 G s O ck of ona ctay S O OTO 101 26 3000VE 10126 3000VE w
12. cable to make the connection Neuralynx Inc 105 Commercial Drive Bozeman MT 59715 Phone 406 585 4542 lt Fax 406 585 9034 www Neuralynx com Revision support Neuralynx com 10 27 2010 Page 10 6 6 Front Panel Lights There are four recessed LEDs on the top section of the DRS 36 which are viewable from the front These top four LEDs indicate the status of the four supplies From top to bottom these LEDs indicate e 5v Headstage Power e 5v Headstage Power e 5v Board Analog Power e 5v Board Analog Power A blue LED in the center of the board labeled HSPwr indicates when the 5v headstage power is supplied to the headstage connector The bottom section of the board contains five recessed LEDs which indicate Boot to show that the board has been properly initialized Accs to indicate that the last DRS 36 command was processed by this board Error to indicate that a DRS 36 Serial String command contained an error D4 a reserved status indicator D5 a reserved status indicator Note that an error does not indicate a failure condition unless it stays lit during system boot up or a time when many reference signal changes are made from the Cheetah User Interface Neuralynx Inc 105 Commercial Drive Bozeman MT 59715 Phone 406 585 4542 lt Fax 406 585 9034 www Neuralynx com Revision support Neuralynx com 10 27 2010 Page 11 7 Board and Parts The following picture shows the majo
13. d This bug causes about 1 in 5000 bytes to be lost or damaged Each DRS 36 command packet consists of 12 characters and 96 fiber optic cable bytes so about 1 in 300 commands will experience an error The format of the DRS 36 commands has been designed so that any missing character will be detected as an error All DRS 36 commands are sent twice from the Cheetah Software to make sure that a damaged command packet will be received at least once either by the first or second command packet 10 Board Numbering and Input Board A D Numbers The Digital Lynx cabinet contains 10 board slots which are populated with Input Boards DRS 36s or a blank slot filler These board positions are numbered from left to right BO through B9 Board Slot 0 MUST be populated with an Input Board Remember that the first A D input on a board is A D 0 The A D input channel numbering is based on Board Slot number and the channel number of the Input Board with the equation A D_Channel Board 32 Input_Number For example Channel 15 on board in slot B2 would be 79 79 2 32 15 Normally the DRS 36 is inserted to the right of the Input Board it is connected to A 64 channel system would have a hardware board configuration of Input Board Slot BO DRS 36 Slot B1 Input Board Slot B2 DRS 36 Slot B3 Therefore the two Input Boards will have the A D channels of 0 gt 31 and 64 gt 95 Neuralynx Inc 105 Commercial Drive Bozeman MT 59715
14. e 6 1 2 Headstage Stimulus Two differential stimulus channel connections are present from the 3M MDR 26 connector to the Headstage 3M MDR 50 connector These stimulus channels Stim1_Src Stim1_Ret Stim2_Src and Stim2_Ret are consistent with all Neuralynx headstages tethers and ERP xx products that incorporate stimulus lines 6 1 3 Animal Ground as a Reference Signal The Animal Ground signal is made by a non current carrying wire connected to the headstage ground plane through the tether cable and is present on the DRS 36 for use as a reference signal The theory for this signal is that it is separate from the normal power ground lines in the tether This Animal Ground signal wire carries no current therefore it should not have any voltage drop along the signal wire and it will be the most faithful representation of the ground voltage on the animal This signal is useful when adjusting Neuralynx Inc 105 Commercial Drive Bozeman MT 59715 Phone 406 585 4542 lt Fax 406 585 9034 www Neuralynx com Revision support Neuralynx com 10 27 2010 Page 7 reference electrodes or when active reference electrodes have become damaged or inoperative This Animal Ground is the same as on other Neuralynx products 6 1 4 Panel Ground as a Reference Signal Panel Ground is the ground plane signal on the DRS 36 board It may be used as a reference signal if needed This is consistent with other Neuralynx products 6 2 Reference B
15. ers perform the same Channel Reference subtraction functions as on the Input Boards prior to A D conversion A Gain of 10 is designed into this section to boost the signal level The inputs to the amplifier are the same differential connections presented to the input board The INA2128 inputs are DC coupled no AC blocking coupling capacitor so it is possible to clip the outputs of these amplifiers by presenting a 500 millivolt difference between the signal and the selected reference Neuralynx Inc 105 Commercial Drive Bozeman MT 59715 Phone 406 585 4542 lt Fax 406 585 9034 www Neuralynx com Revision support Neuralynx com 10 27 2010 Page 27
16. he output channels reference signal Headstage A unity gain low noise buffer amplifier usually placed close to the recording site of an electrode Headstage Signals These are the buffered electrode signals from the unity gain amplifier mounted on the animal They are present on the front panel Headstage Connector Neuralynx Inc 105 Commercial Drive Bozeman MT 59715 Phone 406 585 4542 lt Fax 406 585 9034 www Neuralynx com Revision support Neuralynx com 10 27 2010 Page 5 5 Reference Signal Concepts Neural electrodes contain the desired signal plus a combination of undesired noise error and artifact signals A reference electrode signal ideally is void of the desired signal and only consists of the same error noise and artifact signals To obtain the clean noise free desired signal the reference electrode signal is subtracted from the neural electrode signal normally with electronics All Neuralynx data acquisition products boast an excellent Common Mode Rejection Ratio CMRR specification on neural inputs on either the Lynx 8 Amplifier or the Digital Lynx system of over 100 db a ratio of 100 000 to 1 This design aspect of Neuralynx hardware provides the basis for low noise recording results As wiring PC Board traces connectors and or electronics are added to the signal paths the CMRR specification will be degraded due to parasitic capacitances inductances and circuit resistances The DRS 36 Common
17. i Neuralynx High Density Electrophysiology TX Systems DRS 36 User Manual Digital Reference Selection Board For the Digital Lynx Acquisition System Neuralynx Inc 105 Commercial Drive Bozeman MT 59715 Phone 406 585 4542 lt Fax 406 585 9034 www Neuralynx com Revision support Neuralynx com 10 27 2010 Table of Contents Table OF STT 3 1 Document Revision ISIOEY occcnseccunnced oseceaneieadtenceraccacqupecads cere teseclavccansecanceneemueadiaasente 3 2 VOUT CIV W esmerin sE EE N EE EE E R iene 3 OO ea aac E N E EERE se ese 3 BBS e e T a A Saeed wanteedin cet tea auidd ans bpdesBimasieadte S 5 5 Reference Signal Concepts ss ssgcggzsxe soot rodeagsss redadeg KDO iier eiieeii 6 6 Hardware Cy sss iire inssei seini ade tsa scene tenani a Ea anaa ESEE A TaSi AARE EAA 7 6 1 Headstag npt e Te TTT 7 61 1 lt Ts T POWer cisies ucelaisiecedasceieeuipieeetieeelinieie eee 7 6 1 2 He dstage SUmMmuUlUS ioi iscissi ainis iare aieas eii 7 6 1 3 Animal Ground as a Reference Signal sese eee eee 7 6 1 4 Panel Ground as a Reference Signal sss sees 8 6 2 Reference Bus Block Diagram sss sss eee 8 6 2 1 8 References per DRR 3p sese 8 6 2 2 Global Reference Bus GRB siceencenrentcssenccsasaienncsdenannna teem nacteeicawatncdes 9 6 3 Headstage Input Signal Switching to the Reference BUS sss sese eee 9 6 4 8 Local Reference Bus Signals to use as 32 Channel Rererenceg sees 10 6 5 Connection to the Input Boards sss sees eee 10 6
18. lell awi al SSI SSI gt GEE GEE s S a o wj PBE PEE 25 Use 4 of wire cable for the global bus cable between connectors IS Beba SSH 2 a S S a a Title DRS 36_Global_Bus_Cable Bize Document Number ev A DRS 36_Global_Bus_Cable 1 Date Friday January 05 2007 Bheet 1 of 4 Figure 9 2 Global Reference Cable Pinout 9 1 Headstage Modifications Recently a new revision of the Neuralynx Headstages was released with a modified board layout of the Stimulation and Video Tracking traces In the past a stimulation plug was needed to power all Video Tracking LED s on the Headstages The HS 36 and the HS 18 Cooner were modified so that this plug is no longer needed If you have an older revision Headstage please continue to use the stim plugs as before Please refer to Table for reference Table 1 Headstage HS STIM Plug Requirements for Video Tracking LED s HS 36 HS 27 HS 18 Cooner FWT HS 36 STIM Plug needed HS 27 STIM Plug Needed HS 36 STIM Plug needed on Revision 2 0 or earlier on all HS s on Revision 2 0 or earlier Neuralynx Inc 105 Commercial Drive Bozeman MT 59715 Phone 406 585 4542 lt Fax 406 585 9034 www Neuralynx com Revision support Neuralynx com 10 27 2010 Page 20 9 2 Communications Path from Cheetah As of January 20 2007 there is a known bug in the communications path from the fiber optic cable to the high speed serial receiver MGT on the Digital Lynx Motherboar
19. lot 8 Empty Slot Cover Slot 9 Empty Slot Cover 2 Replace the Flash Card in the System Ace board found behind the rear cover of the Digital Lynx interface box with the Flash Card supplied 3 Move the Blue Jumper Wire to define the number of boards in the Xilinx programming string the source of the programming is from the Flash Card in the above step as shown in the table below Total Input and DRS 36 boards Blue Wire Connector Position 1 not valid as this would be only for 1 input board and no DRS 36 2 1 IB and 1 DRS 14 3 IB DRS IB 13 4 IB DRS IB DRS 12 5 IB DRS IB DRS IB 11 6 IB DRS IB DRS IB DRS 10 7 IB DRS IB DRS IB DRS IB 9 8 IB DRS IB DRS IB DRS IB DRS 8 9 IB DRS IB DRS IB DRS IB DRS IB 7 10 IB DRS IB DRS IB DRS IB DRS IB DRS 6 Neuralynx Inc 105 Commercial Drive Bozeman MT 59715 Phone 406 585 4542 lt Fax 406 585 9034 www Neuralynx com Revision support Neuralynx com 10 27 2010 Page 24 Locating the Blue Wire for Jtag Prog Length Fiber Optic ez Buss Brogramming HW PCA Transciever R Board N Cable R 5 ae Rear of Digital Lynx Interface Box Bulk Power Supply Modules Config DIP Main Fuse l Switch 4amp SB _ Insight Xilinx Development Board also called the Mother Board Note that the Blue Wire is currently covering Pin 12 fora four Board Jtag Programming String Ping Pin 2 Pin2 Pin PinlO Pinl4 Pin pin7 pin Pinl5 Pin pin 3 Figure
20. normally derived driven from individual headstage signals GRB signals are normally buffered before being driven on to the bus and are buffered again after the front panel connector before being used as Input Board Channel reference signals This second rebuffering prevents the parasitic capacitance of the solid state switches used on the DRS from loading the signal lines and degrading the signal 6 3 Headstage Input Signal Switching to the Reference Bus The 38 headstage signals 32 4ref Agnd PGnd each have a 1 to 8 solid state multiplexer switch integrated circuit device This switch chip has a common input which is the headstage signal input and 8 outputs one of which may be switched to the input There is also an enable switch which is used to disconnect the headstage signal from all of the 8 local reference bus signal lines Outputs 1 8 Multiplexer with Enable 8 Input 7 Common v ad S 7 Enable 3 Switch p Figure 6 2 Headstage Input Neuralynx Inc 105 Commercial Drive Bozeman MT 59715 Phone 406 585 4542 Fax 406 585 9034 www Neuralynx com Revision support Neuralynx com 10 27 2010 Page 9 The 8 output pins from the 38 multiplexer chips are tied in common to the 8 respective Local Reference Bus signals This design allows any headstage signal to be connected to any Local Reference Bus signal Note that the enable switch feature in the multiplexer chip allows a no connect for the heads
21. ort Neuralynx com Page 17 8 4 Internal Single Ended Monitor Connector Revision 10 27 2010 This connector will be not accessable by the front panel JSEOUT1 AGnd AGnd 34 SE_Monitors31 33 SE_Monitors30 32 SE_Monitors29 31 SE_Monitors28 30 SE_Monitors27 29 SE_Monitors26 28 SE_Monitors25 27 SE_Monitors24 26 SE_Monitors23 25 SE_Monitors22 24 SE_Monitors21 23 SE_Monitors20 22 SE_Monitors19 21 SE_Monitors18 20 SE_Monitors17 19 SE_Monitors16 18 SE_Monitors15 17 SE_Monitors14 16 SE_Monitors13 15 SE_Monitors12 14 SE_Monitors11 13 SE_Monitors10 12 SE_Monitors9 11 SE_Monitors8 10 SE_Monitors7 9 SE_Monitors6 8 SE_Monitors5 7 SE_Monitors4 6 SE_Monitors3 5 SE_Monitors2 4 SE Monitors 3 SE_MonitorsO 2 me L N2534 6002RB 3M MHC34K ND Figure 8 4 Monitor Output Neuralynx Inc 105 Commercial Drive Bozeman MT 59715 Phone 406 585 4542 lt Fax 406 585 9034 www Neuralynx com support Neuralynx com Page 18 9 Global Bus Cable Figure 9 1 Global Reference Cable The Global Reference Bus GRB Cable connects two or more boards GRB connectors in parallel so that reference signals may be shared between DRS 36 and headstages Each GRB connector on the cable also has a 10 pin connection for the Headstage Stimulus Channel signals The schematic is shown for a two connector GRB cable Neuralynx Inc 105 Commercial Drive Bozem
22. r components of the DRS 36 BackPlane Bus Connector so 4 KE 86 EL ee 5 wv v 5 rm 7 mmn 7 mn F TE TN i K Internal T mmm 7 nmm 7 mmn 7 Single Headstage to Reference oar P Sb sar Pa Pe uar Ba i VUI 1 ugya O ngn i ix Bus Selectio T A aa w 8 E 9 i Buffer Multiplexers l i 1 l L MO e trn ttn trn m Output ve L Conn DU re W x F mnn wmm mi Neuralynx Inc 105 Commercial Drive Bozeman MT 59715 Phone 406 585 4542 lt Fax 406 585 9034 www Neuralynx com Revision support Neuralynx com 10 27 2010 Page 12 8 Connectors 8 1 Headstage Input The Headstage Input Connector is the standard 3M 50 pin MDR connector used throughout other Neuralynx ERP xx patch panels and the Digital Lynx Input Board The wiring on this connector uses 32 input signal pins and 4 input reference signals as well as the standard headstage power animal ground and two differential stimulus channels The wiring on this connector is a superset of signals and will support all Neuralynx headstages Neuralynx Inc 105 Commercial Drive Bozeman MT 59715 Phone 406 585 4542 lt Fax 406 585 9034 www Neuralynx com Revision support Neuralynx com 10 27 2010 Page 13 3M N10250 52E2PC fF 20 AGnd 52 a C eT eis HSIn31 43 4 on L HSIn27 HSin30 dE 4 a ee HSin26 HSin29 46 46 oy Lal HSin
23. s can cause a large lesion 8 3 Differential Output Connector The output signal connector has lines for the 32 differential output signals and 16 ground wires This connector has a mirrored pinout of the Digital Lynx Input Board input connector to allow a simple 80 pin jumper cable to be used to connect the DRS 36 output to the Input Board Neuralynx Inc 105 Commercial Drive Bozeman MT 59715 Phone 406 585 4542 lt Fax 406 585 9034 www Neuralynx com Revision support Neuralynx com 10 27 2010 Page 16 Revision 10 27 2010 JIN2 AGnd AGnd AGnd AGnd Out 31 Out 415 Out 31 Out 15 Out 30 Out 14 Out 30 Out 1 Out 29 Out 13 Out 29 Out 13 Out 28 Out 12 Out 28 Out 12 Out 27 n Out 14 Out 27 ef Out 11 Out 26 a Out 10 Out 26 Out 10 Out 25 Out 9 Out 2 2 Out s Out 24 Po Out 8 Out 24 a Out s AGnd AGnd AGnd AGnd AGnd gt AGnd AGnd g AGnd Out 2 Out 7 Out 23 Out Out 22 i Out 6 Out 22 E TTS Out 21 g Out 5 Out 24 E Out S Out 20 Out 4 Out 20 ka Out 4 Out 19 4 LE Out 19 H Outt 3 Out 18 a Out Out 18 E Ou 2 Out 17 2 ETS Out 17 2 Out 4 Out 16 Out 0 Out 16 Out oO AGnd AGnd AGnd LC AGnd CONN_80_AB RN_S50E_080_P1_SR1_1G Note This is backwards from the IB Input 0 pin connector because of the way the cables are made Figure 8 3 80 Pin Differential Output Neuralynx Inc 105 Commercial Drive Bozeman MT 59715 Phone 406 585 4542 lt Fax 406 585 9034 www Neuralynx com supp
24. tage signal The intelligence on the DRS 36 board will allow only one headstage signal to be connected to any individual Local Reference Bus signal line 6 4 8 Local Reference Bus Signals to use as 32 Channel References The same 8 1 Multiplexer IC is used to individually select one of the 8 Local Reference Bus signals LRB for each of the 32 output channels reference signals 8 Local Ref Bus Output Reference Ref Signal Inputs for 1 of 32 channels 8 1 8 Multiplexer 7 with Enable 6 5 v 4 3 Common 2 Enable Switch Figure 6 3 LRB Signals Remember that the output of the DRS 36 connects directly to the input of the Digital Lynx Input Board AC or DC coupled versions There are 32 differential inputs on the Input Board and 32 differential outputs on the DRS 36 and each channel has a Signal and a Reference The eight inputs on the left connect to the LRB and the Common pin on the right connects to an output channel s reference signal The output Enable Switch shown is set to be closed enabled 6 5 Connection to the Input Boards The connection of the 32 differential output channels to the Input Board is normally done with a short 80 pin dual ribbon cable jumper The output connector is a high density 80 pin connector the same part as the Input Board s differential input connector with the exception that the DRS 36 connector is mirrored from the Input Board s connector to allow the use of this simple
25. to these lines and there is a continuous connection from the ERP xx or DRS 36 from the input connector through the tether and headstage through to the Electrode Interface Board mounted on the animal or microdrive The Global Reference Bus Cable will have the standard 10 pin connector mounted on a short wired connection N10226 52B2PC 6 L e D nat 2 L il AGnd 26 13 Gbl_Ref_Bus_Cable_ Connect HS Power Neg 25 a 12 HS Power Pos S2 Ret 24 24 11 11 S2 Src S1_Ret 23 23 10 10 Si Sre AGnd 22 22 9 9 AGnd AGnd 21 8 Global_Refs8 AGnd 20 0 di Global _Refs7 AGnd 19 19 6 6 Global_Refs6 AGnd 18 18 5 5 Global_Refs5 AGnd 17 17 4 4 Global_Refs4 AGnd 16 16 3 3 Global_Refs3 AGnd 15 15 gt 2 Global_Refs2 AGnd 14 14 1 1 Global_Refs1 a n AGnd 27 27 Figure 8 2 LRF Connector Neuralynx Inc 105 Commercial Drive Bozeman MT 59715 Phone 406 585 4542 lt Fax 406 585 9034 www Neuralynx com Revision support Neuralynx com 10 27 2010 Page 15 Note that the Stimulus Channel lines can be used for two purposes 1 to deliver stimulus current to the animal and 2 to power the Video Tracker LEDs on a headstage If your experiments or laboratory use both Video Tracker LEDs and direct brain current stimulation you MUST make sure that the Stimulation Channels do NOT have the video tracker LEDs power supplied to stimulation electrodes in the brain Thi
26. us Block Diagram The following figure shows the various switches buffers and busses used on the DRS 36 The various reference bus sections are always a maximum of 8 signals wide Front Panel Headstage Connector Front Panel GRB Connector Output Connector connects to Input Board 32 differential signal connector Headstage GRB Front Panel Input Board 37 input signals Connector Output Connector Panel Ground 3M MDR 26 32 Diff Output Signals 64 total 2 b L 2 32 Headstage Signals A1 gt 16 p and B1 gt 16 z z Z a i 2 8 8 GRB signals may be k 8 GRB Switches sourced or input m 8 Controlled f Array of 38 Solid State 1 8 3 Re Buffer Amps E 4 8 Local Ref Bus BREl Array of 32 Solid State multiplexer switches eels with output a Buffer Amps f Sne g multiplexer switches enables 8 Local Ref Bus signals which are used for Selection of possible 8 headstage differential output signal reference selection signals to be used as references Figure 6 1 Block Diagram of DRS 6 2 1 8 References per DRS 36 The DRS 36 supports up to 8 reference signals to be used on the board This means that the 32 output channels connected to the Digital Lynx Input Board can have 8 different reference signals This would equate to one reference per 4 channel Tetrode This has proven to be more than enough for most normal spike
27. x 10 Down Up Up Down Up Up Up Up 5 Set the NumBoards config file parameter in the Digital_Lynx cfg file This should be set to the number of boards in the system It MUST correspond to the number of boards setting of the red DIP Switch above Jn this setting do not count a DRS board if it is in the last rightmost slot of the Digital Lynx 16 10X differential Amp Section and Internal Monitor Output Connector The Monitor Output connector provides user connection to the 32 output channels which are buffered amplified 10X and converted to single ended output signals This Monitor connector mounted on the PC Board and is not accessible on the front panel These signals can be used for audio or oscilloscope monitoring This section of the board contains 32 differential Instrumentation Amplifiers one for each channel with a gain of 10 It is possible to connect a 34 pin ribbon cable to this internal connector and pass the ribbon cable between front panel board segments and use this for your own purposes Please note that improper use of these signals may induce noise or other artifact into the system See the section on Connectors for more details 17 INA 10X Gain Section The Single Ended Output can be used as a monitor output for all 32 differential output put channels The same instrumentation amplifier INA2128 used on the Digital Lynx Input Boards are connected to the 32 output channels on this board These Instrumentation Amplifi
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