Model PS9009 TC Prog. High Voltage Power Supply user manual
Contents
1. INGE VVGIQINE ici daaardras OR aa ada a ap BEEG 3 5 kg 7 7 lb Shipping Weight occurred a ena oe 6 0 kg 13 2 Ib Dimensions Dx WxH ocococcccccccccco 33 x 27 x 11 cm 12 9 x 10 6 x 4 3 in Installation Category ococcccocccco tent tneeee Type 2 Power Requirement 0 0 cece eee eee eee ene 110 220 VAC 50 60 Hz 750 W Mains fuse rating 110 E TO Vims Operation essa rr ted haw wee ete Coes T 4 0 A 250 V 220 20 vrms operation 1 eee T 2 0 A 250 V Maximum Voltage cerae a ngage anh ee e 3 000 V Maximum POWEeF wc cca ae ee eee cea ee wea hehe ae hae cea nee ee Ee es 300 W Maximum Current 2 00 0 cc eet ene 300 mA Regulatoria ica dar lt 1 ACCUIACY ninia a As 1 5 full scale for each display Number of Output Terminals Two recessed sets of 4 mm output connectors Safety Interlock Load sensing shut down on disconnect Key activation necessary to begin voltage generation In the event of shut down due to power interruption automatic restart is provided TIMOR eri tian Hera a a ea 0 to 99999 min Ground Leakage Leakage of 500 pA will interrupt the generation of high voltage MIMS GMAT ONE cairo ta op Bisa Soe ETOR ase ncaa fake aM a A og ede aaa ee ie 99999 V h Programmability o o ooooo o 8 programs each with 10 steps 16 single step programs and 1 low current program with 10 steps 13 14 Additional Information 5 3 Warranty Lif2. a net value of 25 mA Rule 3 2 The reason Cell A and Cell B have different current readings is due to the difference in resistance between Cells A and B Rule 2 3 8 Using Automatic Crossover Certain electrophoretic techniques require the careful adjustment of operating limits and the utilization of the automatic crossover feature of this power supply Automatic crossover involves a transition from one mode of operation e g constant current to another mode of operation such as constant voltage Semi dry electroblotting exemplifies the utility of this feature Semi dry transfer chambers contain two closely spaced parallel electrode plates A sandwich consisting of buffer saturated filter paper sheets on the outside and a gel and a charged transfer membrane on the inside is assembled and placed between the electrode plates Typical protocols suggest that the transfer should be carried out at a constant current As the transfer process progresses the buffer in the two filter paper layers heats up and begins to break down This breakdown leads to an increase in the overall resistance between the two plates In the constant current mode the increase in resistance leads to a voltage increase Left unchecked the increasing voltage can eventually lead to arcing which would damage the electrode plates the gel and the transfer membrane To eliminate the arcing problem the voltage should be set at an operating l
3. For example submarine chambers use agarose gels to separate DNA or RNA fragments while a DNA sequencing chamber is almost always used to separate DNA in a denaturing polyacrylamide gel In each case the voltage milliamp and wattage requirements are well defined within a reasonable range of values In cases such as these the user can safely assume that the manufacturer has designed the chamber to withstand the voltage and heat energy necessary to perform the electrophoretic separation when standard protocols are followed Some types of electrophoretic chambers are specifically designed to be multipurpose devices For example a vertical slab gel chamber could be used for anything from DNA sequencing to isoelectric focusing depending on the gel type and buffer system used Choosing safe operating limits for a chamber of this type requires a higher degree of caution Chamber manufacturers normally rate their product for maximum voltage and or maximum wattage Whenever possible contact the manufacturer and request this information Whether you use special purpose or multipurpose chambers the maximum operating temperature the chambers will withstand is a critical aspect of safe operation Most electrophoretic chambers with a few notable exceptions are made of acrylic and plastic and should operate below a temperature of 35 C If there is a lack of information about the capabilities of the chamber being used regular monitoring of the operating t
4. Troubleshooting Guide 4 2 2 Ground Leakage Message LEAKAGE fault press STOP to resume The LEAKAGE Fault Detection message indicates that some of the current output is flowing to earth ground This situation can be hazardous Switch the AC Mains Power ON OFF Switch to the OFF position Check the leads and connections and make sure that no buffer is leaking from the electrophoresis chamber onto the bench before proceeding Contact the TECH LINES if the problem persists 4 2 3 Power Fault Message POWER fault press STOP to resume The POWER Fault Detection message indicates that there is a POWER CIRCUIT FAILURE This may be caused by internal excess current in the power supply Reset the power supply by switching off the AC Mains Power ON OFF Switch to the OFF position and then ON again Contact the TECH LINE if the problem persists 4 2 4 Memory Lost Message MEMORY lost press STOP to resume The Memory Lost message indicates that the permanent memory has been lost due to a circuit failure or a problem in the software Reset the power supply by switching off the AC Mains Power ON OFF Switch to the OFF position and then ON again Contact the TecH LINE if the problem persists 4 2 5 Power Restart Message POWER IS COMING ON press STOP to resume The Power Restart message indicates that there has been a power failure of the AC Mains The automatic restart of the unit will occur in 10
5. set value for the MODE that is not limiting Load and gel resistance are changing Disregard slight fluctuations Display voltage and current will stabilize as gel equilibrates Increase or decrease W set value by 1 or 2 Watts to stabilize When the temperature limit is reached the power supply shuts off both voltage and current output Check the MinR display and then electrophorese the gel for the total time displayed on this timer Make sure that the correct voltage limiting value was set Make sure that the temperature probe is properly connected to power supply Increase the temperature limit value Decrease the electrophoresis parameters Make sure that the probe in in complete contact with the glass plates ns 12 Related Products Product Model PS 3002 Electrophoresis Power Supply Model 4001 Electrophoresis Power Supply Model 4001P Programmable Electrophoresis Power Supply Model S2 Sequencing Gel Electrophoresis System Model SA 32 Sequencing Gel Electrophoresis System Model SA 60 Sequencing Gel Electrophoresis System Model SA 88 Sequencing Gel Electrophoresis System Model V15 17 Vertical Gel Electrophoresis System GeEL Mix 6 6 Sequencing Gel System GeL Mix 8 8 Sequencing Gel System dsDNA Cycle Sequencing System 100 reactions HORIZON 11 14 Horizontal Gel Electrophoresis Apparatus HORIZON 20 25 Horizontal Gel Electrophoresis Apparatus Horizon 58 Horizontal Gel Electrophoresis Apparatus Temperatu
6. shutoff and are identified by S on the display panel This symbol indicates that for each program there is only a single programmed step Each program is maintained in the memory of the power supply Electrophoresing a vertical polyacrylamide sequencing gel at 60 W constant power for 3 h 1 Switch the AC Mains Power ON OFF Switch to the ON position An alarm sounds to signify the start up of power 2 Press the Start Stop keypad to silence the alarm The last program used is displayed 0000V 000mA 000W PO1 00000 VHR 0000 min S 3 Press the Program keypad and enter the program number using the numerical keypad 4 To enter the setting parameter press the keypad of the parameter to be changed and then using the numerical keypad enter the desired value Values which are out of limits are not accepted 5 Enter 3000 V 300 mA 60 W entered 060 99999 VHR and 180 min entered 0180 The V mA and VHR functions are set at maximum so they are not limiting factors After each value has been entered the display remains for 5s and then changes to display all zeros Press any keypad to return to the program that is being entered 3000 V 300mA 060W PO1 99999 VHR 0180 min S 6 Press the Start Stop keypad to start electrophoresis The Model PS9009 TC Power Supply has automatic crossover with the limiting parameter being indicated by the blinking display Operating Instructions Note A value for both the VHR and
7. that both the main receptacle and the power supply plug have the proper three wire grounded or earthed connections Overview DAN IGER HIGH VOLTAGE ure cun OLOGES 21 23 zZ 2 AN Oa KR WD PS 9009 TC TEMPERATURE CONTROLLED 3090 Vols 99999VHR 99999min SH PROBE 005080500000 J 300 Watts 22 13 15 17 14 16 DESCRIPTION 18 AC Mains Power ON OFF Switch Voltage Output Display Current Output Display Power Output Display Volt Hour Display Timer Display Program Number STEP Number Resume Switch Start Stop Switch Start LED Stop LED Volt Selector mA Selector Watt or C Selector VHR Selector MIN Selector Program Selector Previous Step Selector Next Step Selector High Voltage Output Jacks Numeric Keypad Temperature Probe Output PREV T Microprocessor controlled 3000V 250mA 250W P23 PROGRAMMABLE ELECTROPHORESIS POWER SUPPLY A 11 12 19 9 20 10 FUNCTION Turns on the main power Displays actual or set Volts Displays actual or set mA Displays actual or set Watts Displays actual or set VHR Displays actual or set MIN Displays actual or preset program Displays actual or preset step Resumes timer and VHR values Starts or stops High Voltage Output Green LED indicates High Voltage on Red LED indicates High Voltage off Permits entry of volt set point Permits entry of current set point Permits entry of Watt or temperature set point Perm
8. GIBCOBRL Instruction Manual Model PS9009 TC Programmable Electrophoresis Power Supply CAT NOS 31067 242 10556 017 Lire TECHVOLOGIES Essential Technologies for the Science of Life Table of Contents Ts Introductio aire inti Gand ei aes 1 1 1 Notice to CUStOMED ooooocincccinccioninonncnnnnnnnnnnnn cnn cn non crac cnn crac narran nana 1 112 Wario Serene i ae 1 2 TOV esse here ieee tee cece 2 21 Descripcion 2 22 Serpiente it ra rasta 2 3 Operating INStruCtiONS ooooocicicicnicninicnininninnnnnnneencennnss 4 3 1 Programming and Operating a One Time Single Step Program 4 3 2 Programming and Operating a Sequential Program csccsceeseeeeeees 5 3 3 Programming and Operating the Low Current Program cssccessseeeeees 5 3 4 Temperature Control Program c cecceeecceeseeeeceeeeseeeeeeeeeeeseeeesaeeseaeeeeesaas 5 3 5 Setting Safe Operating Limits eee cece eeeeeeneeeeeeeeeeeeeeeeaeeseaeeeaeeeaas 6 3 6 Relationships Between Volts Milliamps Watts and Chamber Resistance cccceeeceeeneeeeeeeeeeeeeeeeeaeeeaeeseaeeeaeeseaeeeaeeeeaeens 7 3 7 Running Multiple Chambers c ceecceeseeeeeeeeeeeeeeeeeeeeeeeeeeeeeeaeeseaeeeaeeeaas 7 3 8 Using Automatic CroSSOVelr c ooocnncnnccnoccnnocnnncnnnnn cnn cnn cc nnr cnn nr rnn rancio 8 3 9 IMO QPANOM cesta ageeanaeed 8 4 Troubleshooting Guide coccion 9 4 1 Display Messages cceseseeeeeeceeee
9. U The EU is currently comprised of 15 member countries This laboratory apparatus is identified with the CE mark This mark indicates that the product complies to the following EU Directives and Standards Application of Council Directive s 73 23 EEC Low Voltage Directive 89 336 EEC Electromagnetic Compatibility Standards EN 61010 1 1993 Product Safety EN 50081 1 1992 Emissions EN 50082 1 1992 Immunity EU Representative Life Technologies Ltd EU Address 3 Fountain Dr Inchinnan Business Park Paisley PA49RF Scotland A copy of the Declaration of Conformity certificate is available upon request PartNo 50892 Lot No KJNP01 0898
10. e Technologies Inc warrants apparatus of its manufacture against defects in materials and workmanship under normal service for one year from the date of receipt by the purchaser This warranty excludes damages resulting from shipping misuse carelessness or neglect Life Technologies liability under the warranty is limited to the repair of such defects or the replacement of the product at its option and is subject to receipt of reasonable proof by the customer that the defect is embraced within the terms of the warranty All claims made under this warranty must be presented to Life Technologies within one year following the date of delivery of the product to the customer This warranty is in lieu of any other warranties or guarantees expressed or implied arising by law or otherwise Life Technologies makes no other warranty expressed or implied including warranties of merchantability or fitness for a particular purpose Under no circumstances shall Life Technologies be liable for damages either consequential compensatory incidental or special sounding in negligence strict liability breach of warranty or any other theory arising out of the use of the product listed herein Life Technologies reserves the right to make improvements in design construction and appearance without notice 5 4 Declaration of Conformity and CE Mark Note The information outlined in this section applies only to customers located in the European Union E
11. eeeeseeeeeeeeeeeesuseneneeeeseseeesessenesenenenees 9 4 1 1 Start Up MeSSage coooccncccionccocccoonnconcnonancnncnnnnn nan cn nn cnn a 9 4 1 2 End of Run Display 00000 eee ee eeseeeeeseeeeeneeeeeeneeeeeneeeeeneeeeenneeesnaeees 9 4 2 Fault Detection Messages ooooocinoccccnonccononnnononncnnnnnnnnnnn cnn nano nana n nn nan ncrnn nn 9 4 2 1 No Load Detection Message cooococinoccccnonncononnnononnccnnnnnnnnnrnnnnan cnn 9 4 2 2 Ground Leakage Message ccooconcccconccoocccocnconcncnnncnncnnanccnncnnnncnnos 10 4 2 3 Power Fault Message ooococinoccccnnonccnonnccnonnncnnnnnnnnnn cnn non nnnnnnnnnnnnnrs 10 4 2 4 Memory Lost Message ococcooccccnoccconoccncnoncncnnonncnnnn cnn nono n cnn nnnnnnnos 10 4 2 5 Power Restart Message ccoooccccnoncconncnccnonnncnanononnnnnnnnonnnnnnnnnnnnnos 10 4 3 General Troubleshooting ococinccnnccnnninnccnnncnconncnnnccnnn nono nnnncn nan nnnncnnnncnns 11 5 Related Products o oo ccccecceceseeseseeseeesseceeseeeeeeseeseseeeseeeeseeensees 12 6 Additional Information cece eeeeeeeeeeeteeteteeteteeeeeenees 13 6 1 Care and Handling 62 Specifications iii ira 63 Warranty 6 4 Declaration of Conformity and CE Mark ccceeceeeeeseeseeeteteeeeeeteneees 14 Figure 1 Model PS9009 TC Programmable Electrophoresis Power SUPPlV coimas dell de a 3 GeL Mix Horizon TECH LINE and the Life Technologies logo are marks of Life Technologies Inc gt gt Introduction 1 1 Notice to Cu
12. emperature is recommended This should be accomplished without coming into physical contact with the chamber when voltage is applied The following procedure illustrates how normal operating conditions can be determined and how this information can be used to choose safe operating limits for agarose gel electrophoresis in a submarine chamber at a constant voltage of 125 V Operating Instructions Note When operating in constant current or constant power and setting a limit for voltage select a value which is greater than the actual value by 10 or 25 V whichever is greater 1 Adjust the operating limits to 125 V 300 mA 300 W 2 Start electrophoresis and note the mA and W values when the voltage has reached 125 V and begins to blink 3 Adjust the mA and W settings so that they exceed the actual values by 10 or 10 mA and 5 W whichever is greater Thus the SET and ACTUAL values may look like this SET ACTUAL 0125V 055mA 011 W 0125V 045mA 006W In the procedure listed above we first determined the actual output wattage and milliamps for operation at 125 V and then chose operating limits that were at a slightly higher level than those indicated for mA and W This approach ensures that the maximum output from the power supply will never exceed the normal operating conditions by more than 10 Some users prefer to set up the chamber switch the power supply s output ON and adjust the operating limits be
13. fore the samples are actually loaded Once the user has confirmed that the chamber and the power supply are functioning properly and determined the safe operating limits the power supply s output is switched OFF and the samples are loaded Since the power supply will remember the settings of the operating limits the output can be switched back ON without further adjustments 3 6 Relationships Between Volts Milliamps Watts and Chamber Resistance Three fundamental concepts form the basis for understanding the relationship between volts milliamps and chamber resistance When combined with the power formula they also define watts 1 A movement of free electrons from atom to atom forms an electric current which is measured in milliamps mA or amps A 2 Electrostatic lines of force between two different charges produce a pressure that can move electrons measured in volts 3 All substances oppose the movement of electrons to some extent and are said to have resistance measured in ohms These three factors are always present in any operating electric circuit It is possible to incorporate them into one inclusive statement Ohm s Law The value of the current that will flow in any circuit will be directly proportional to the value of the voltage applied and inversely proportional to the value of the resistance or amps volts resistance combined with The power formula volts x amps watts where 1 amp 1 000 mA Togethe
14. he power supply automatically stops when the total for the vh equals 330 regardless of the fluctuations in the voltage Troubleshooting Guide 4 1 Display Messages 4 1 1 Start Up Message GIBCO BRL press STOP to resume This is the start up message when the power supply is first turned on An alarm will also sound Press the start stop keypad to display the last used program 4 1 2 End of Electrophoresis Display regulation END press STOP to resume Power output has stopped at the end of the last programmed step Change the step or program to continue 4 2 Fault Detection Messages WARNING This notice and the messages below alert you to potentially dangerous situations CAUTION This notice means serious damage may occur to your power supply or chamber 4 2 1 No Load Detection Message LOAD fault press STOP to resume The LOAD Fault Detection message and the continuous tone alarm indicate that the circuit between the power supply and the electrophoresis apparatus is not closed Check that all of the leads are connected properly and press the start stop keypad to resume An open connection could be caused by any of the following conditions 1 a broken electrode within the electrophoretic chamber 2 a broken wire within the connecting cord 3 insufficient buffer levels in the electrophoretic chamber or 4 electrochemical energy stored within the electrophoretic chamber 10
15. imit which is below the threshold needed for the arc As the voltage increases it will eventually reach the predetermined operating limit At this point the power supply will automatically cross over from the constant current mode to the constant voltage mode of operation As the transfer is completed the current will gradually diminish 3 9 Integration The conductivity of the buffer in the electrophoresis chamber can change during the course of a run and result in changes in the voltage current or wattage being applied Integration of the output over a given time period takes such changes into account by monitoring volt hours or milliamp hours or watt hours The vh integration method is used most frequently since the rate of migration of molecules through gels is proportional to the voltage that is being applied The way this feature can be used is described as follows If we run an experiment at constant voltage at an output of 110 V for 3 h we will accrue 330 vh We know this is true since the voltage was held constant for 3 h During the course of this same experiment the output current varied from a maximum of 50 mA to a minimum of 25 mA Now suppose we wish to repeat the experiment at constant current but we want the same effect as 3 h at a constant voltage of 110 V At constant current we know the output voltage must vary since the current varied when we performed the experiment at constant voltage lf we select a vh limit of 330 vh t
16. in SO 7 Press the Next keypad to switch to the next step to be entered 8 Enter 3000 V 30 mA 300 W 99999 VHR and 90 min on step S1 The V W and VHR functions are set at maximum so they are not limiting factors 3000V 030mA 300W P21 99999 VHR 0090 min S1 9 Press the Start Stop keypad to start electrophoresis 10 At the end of the 15 min first step in the program the power supply switches automatically to the next step At the end of the last programmed step the power supply automatically shuts off the voltage and current to the electrophoretic chamber 3 3 Programming and Operating the Low Current Program Program coded P28 is a dedicated low current lt 1 mA program that is operated and programmed in the same fashion as the other multiple step programs It is not used for sequencing or other applications requiring the use of high current 3 4 Temperature Control Program The Model PS9009 TC Power Supply is designed for automatic temperature regulation as well as that for Voltage Current and Power The optional temperature probe maintains constant temperature during electrophoresis by reducing or stopping the voltage and current output when the set temperature is surpassed Operating Instructions Note The temperature control option can be used with any of the programs stored in the memory of the Model PS9009 TC Power Supply Once the temperature drops below the set point both voltage and cur
17. ire receptacle with an adapter This could create a serious electrical hazard for persons using the unit Use the correct voltage AC power outlet for the power supply Be sure to always plug a 110 VAC power supply into a 110 V line and use a 240 V line with a 240 VAC unit Overview 2 1 Description The Model PS9009 TC Programmable Electrophoresis Power Supply is intended to be used with electrophoretic devices that operate below 3000 V and up to 300 W and or 300 mA DC The unit includes two sets of output sockets which operate in parallel to provide output in constant voltage constant milliamps or constant power see figure 1 This power supply has the ability to restart itself in the event of a power failure 2 2 Set Up Unpacking the Unit Unpack and inspect the power supply unit carefully for any damage Do not use the unit if it is damaged If damage is found save the packing material and report the problem to Life Technologies Customer Service Location Make sure the unit is set up in a location where it is protected from physical damage moisture corrosive agents and extreme temperatures Make sure the fan at the rear is not obstructed leave about 20 cm of space behind the unit The unit should be readily accessible for safe operation Connection with the AC Mains Connect the unit to the AC mains carrying the appropriate specified voltage V in accordance with the rating label affixed to the back of the unit Ensure
18. its the entry of Volt Hour end point Permits the entry of Time end point Permits the entry of program number Previous selection of steps Next selection of steps RED POSITIVE BLACK NEGATIVE Permits entry of all parameters Connection of the external surface probe Figure 1 Model PS9009 TC Programmable Electrophoresis Power Supply Note The Low Current Program P28 can not be used for DNA sequencing Note A value for both the VHR and minutes must be entered for the power supply to operate Operating Instructions The touch key pads on the front of the Model PS9009 TC Programable Electrophoresis Power Supply figure 1 are used to program and run the unit The power supply can be operated in any of three different modes Constant Voltage Constant Current or Constant Power with automatic crossover The power supply can also be run with temperature regulation The power supply stores up to 24 different programs which are arranged into three different types Programs coded PO1 to PO8 and P11 to P18 are one time single step programs Programs coded P21 to P27 are multiple step programs and can have up to 10 steps The program coded P28 is designed for low current operation This is a multiple step program for operation lt 1 mA 3 1 Programming and Operating a Single Step Program There are 16 available single step programs coded P0O1 to PO8 and P11 to P18 that can be set These programs include automatic
19. minutes must b entered for the power supply to operate Electrophoresis stops at the end of the programmed time or can be manually stopped by pressing the Start Stop keypad Press the Start Stop keypad to resume electrophoresis at the point where it was stopped 3 2 Programming and Operating a Multiple Step Program There are 7 available multiple step programs coded P21 to P27 that can be set Each program can have up to 10 individual steps which are identified by SO to S9 on the display panel Running a program to electrophorese an SDS PAGE gel first at a constant current of 15 mA for 30 min and then at a constant current of 30 mA for 90 min 1 Switch the AC Mains Power ON OFF Switch to the ON position An alarm sounds to signify the start up of power 2 Press the Start Stop keypad to silence the alarm The last program used is displayed 3 Press the Program keypad and enter the program number using the numerical keypad 4 Using the next and previous keypads select the step to be entered SO to S9 0000 V 000mA 000W P21 00000 VHR 0000 min SO 5 To enter the setting parameters press the keypad of the parameter to be changed and then using the numerical keypad enter the desired value Values which are out of limits are not accepted 6 Enter 3000 V 15 mA 300 W 99999 VHR and 15 min on step SO The V W and VHR functions are set at maximum so they are not limiting factors 3000V 015mA 300W P21 99999 VHR 0015 m
20. r these two formulas define the relationship between volts milliamps watts and chamber resistance 3 7 Running Multiple Chambers This power supply is equipped with two sets of 4 mm output connectors which are connected in parallel because 1 The voltage is applied equally to all branch paths in a parallel circuit 2 The current flow in the branch paths of a parallel circuit is determined by the resistance of the individual paths Operating Instructions 3 The sum of the currents entering the branch paths of a parallel circuit is equal to the sum of the currents leaving the branch paths of a parallel circuit A practical example of this is described as follows The power supply is connected to two identical horizontal submarine electrophoresis chambers cells A and B The power supply output is adjusted to 100 V at constant voltage and the current display indicates 60 mA By applying the three rules for parallel circuits we can determine the following information 1 The voltage applied to both Cell A and Cell B is 100 volts Rule 1 2 The sum of the currents flowing through Cell A and Cell B is equal to 60 mA Rule 3 Switch OFF the power supply and momentarily disconnect Cell B Switch the power supply back ON and note how the output current reading drops to 35 mA From this the following information can be derived 1 The current flow through Cell B is equal to 60 mA 35 mA
21. re Probe Replacement Parts Fuse T 4 0 A 250 V Fuse T 2 0 A 250 V 31067 234 31067 069 31067 119 21105 010 31096 019 31096 035 31096 050 21080 015 15543 010 15545 015 18196 014 11068 012 21069 018 41060 013 31067 291 31067 275 31067 283 Additional Information 5 1 Care and Handling This power supply uses all solid state components and should require no maintenance or recalibration under normal use If the unit must be returned for repair contact Life Technologies Customer Service Department for shipping instructions Please include a full description of the problem As with any laboratory instrument adequate care ensures consistent and reliable performance After each use wash all components gently with water and nonabrasive soap or detergent and rinse well in deionized water Wipe dry with a soft cloth or paper towel or allow to air dry Do not allow water to enter the case To remove grease and oils use a light application of hexane kerosene or aliphatic naphtha Never use abrasive cleaners window sprays or rough cloths to clean the components as these can cause surface damage Additional cautions e Do not autoclave or dry heat sterilize the apparatus or components e Do not expose the apparatus or components to phenol acetone benzene halogenated hydrocarbon solvents or undiluted laboratory alcohols e Avoid prolonged exposure of the apparatus or components to UV light 5 2 Specifications
22. rent output is resumed at the preset levels The use of the temperature regulation mode prevents the activation of the power Watt regulation mode 1 Switch the AC Mains Power ON OFF switch to the ON position An alarm sounds to signify the start up of power 2 Press the Start Stop keypad to silence the alarm The last program used is displayed 3 Press the Program keypad and enter the program number using the numerical keypad 4 Program the desired parameters for electrophoresis Instead of programming Watts press the W C keypad a second time The display shows __ _ C instead of _ W Enter the desired upper temperature limit for the gel The range goes from 00 to 99 C For example 40 C would be entered as 040 0000 V O000mA 040 C P01 00000 MinR 0000 MinT S The display MinR is a timer that is dedicated for increments of minutes while the Model PS9009 TC does not supply current or voltage This timer works only when the voltage and current are turned off in the temperature regulation mode The main timer MinT remains operational at all times during the operation of the power supply and provides the automatic termination of the voltage and current output After the electrophoresis has automatically stopped it may be necessary to continue electrophoresis for the time that the voltage was discontinued 3 5 Setting Safe Operating Limits Electrophoretic chambers are generally designed for a relatively specific purpose
23. s at which time the audible alarm will stop The power supply will restart with the preset values Troubleshooting Guide 4 3 General Troubleshooting Many procedural and operational problems can be solved by carefully following the instructions in this manual Some suggestions for equipment and procedural troubleshooting are given below Should these suggestions not resolve the problem or if you have questions regarding procedures call the TECH LINE numbers listed on the back cover of this manual If you need to return the unit for repair contact the Customer Service Department or your local distributor for shipping instructions Include a full description of the problem Problem Display fails to illuminate when the power supply is turned on Desired MODE not flashing Two different MODES are blinking alternatively Under constant power MODE W output displays are not stable When using the temperature control MODE the separation is not complete Temperature Display shows symbols instead of temperature Voltage is shut off during electrophoresis Display actual temperature is not stable Comments Make sure that the AC Mains power cord is plugged into a functioning outlet Check the AC Mains fuse on the back of the power supply Replace if blown Increase the output setting for the MODE that is limiting until the desired MODE is flashing Settings for both parameters are too close to actual output Increase the
24. stomer This product is authorized for laboratory research use only The product has not been qualified or found safe and effective for any human or animal diagnostic or therapeutic application Uses for other than the labeled intended use may be a violation of applicable law 1 2 Warnings 1 o 10 DANGER HIGH VOLTAGE This power supply has been designed for use as a source of DC power for electrophoresis It is capable of generating lethal currents This unit should always be operated with extreme caution Careless handling could result in electrical shock Never operate damaged equipment Do not use the unit without the cover in place or with any possible short circuit If the power supply emits smoke or blows the main fuses turn off the power supply and disconnect the AC line and power cord Do not operate with connecting cables that have exposed live wires Always turn off the power supply before connecting or removing the power cords or moving an electrophoresis apparatus Handle one power cord at a time with one hand only when connecting or removing DC power cords at the power output terminals Do not immerse this unit in water Do not operate the unit in a damp humid atmosphere or in a fashion where condensed moisture may short out electrical components Do not connect the output to earth ground The AC power cord has a three pronged plug which must be connected to a grounded line voltage receptacle Do not use a two w
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