- YMC America, Inc.
Contents
1. e g peak tailing loss of theoretical plate number The extra column volume has a great impact on band spreading To minimize the influence of band spreading on chromatographic performance especially when using columns in 2 1 mm I D the LC system should be optimized as described below gt The shortest possible length of tubing with narrow inner diameters tubing less than 0 15 mm 0 006 inch I D is recommended should be used for the connection from the injector to the column and from the column to the detector Make sure not to have a void in the connection gt Use a detector equipped with low volume flow cell designed for the narrow bore column gt Use an injector for the narrow bore column and a low volume sample loop A sampling rate and a detector response time constant should be optimized to acquire more than 10 data points across a peak We recommend a sampling rate of about 10 points per second or higher and a detector response of 0 1 s or faster to detect the earliest eluting sharp peak properly 4 Shipping solvent Indicated in the COLUMN INSPECTION REPORT Replace with this solvent for storage When replacing a mobile phase containing buffer salts additives extra care must be taken to prevent salt precipitation UM140221AE 1 2 YMC USER S MANUAL 5 Mobile phase The correct direction of the solvent flow is indicated by an arrow on the column identification label Aqueous or non aqueous solvent can be used as2. a mobile phase Repetitive replacement among solvents with large difference in polarities might degrade the column performance Common organic solvents such as acetonitrile methanol and tetrahydrofuran THF are recommended for regular use When using THF as a mobile phase be mindful of the solvent resistance of your system and tubing PEEK parts are especially unsuitable for use with THF Recommendations of pH for column use are shown in the specifications table in section 2 When using the column at pH near the upper or lower limit the column lifetime will shorten under certain conditions by temperature and mobile phase composition 6 Column cleaning general method Flush the column with solution containing a higher ratio of organic solvent for washing out the compounds that are strongly retained in the column after using mobile phases not containing buffer salts additives Usable concentration of organic solvent is up to 100 A cleaning solution containing THF might be effective when removing highly hydrihobic lipid soluble substances that are adsorbed onto the gel When using mobile phase containing buffer salts additives first replace with a water organic solution containing no buffer salts additives A ratio of water to organic solvent should be set at the same proportions as a mobile phase Then flush the column in accordance with the method described above Mobile phase containing about 50 mM or less buffer salts additives can be replac
3. YMC USER S MANUAL Column Care and Use Instructions Meteoric Core series Meteoric Core C18 Meteoric Core C18 BIO Meteoric Core C8 1 Introduction Thank you for purchasing a Meteoric Core series column for high performancef ultra high performance liquid chromatography HPLC UHPLC Meteoric Core is core shell silica based reversed phase column suitable for ultrafast analysis with high resolution Meteoric Core series columns which are manufactured under highly controlled conditions must pass a series of stringent tests before being accepted for shipment Please refer to the column inspection report To ensure optimal performance and durability of the column please read these instructions carefully before using this column 2 Specifications Usable l Particle Pore size Usable Packing material C pressure size um nm pH range MPa Meteoric Core C18 Meteoric Core series Meteoric Core C18 BIO 2 1 1 5 10 0 Meteoric Core C8 B 5 1 5 9 lt The style of column endfitting is Parker style Port depth ca 2 mm 0 09 inch pm Tubing and connector SA a Column Port depth 7 5 5 3 Recommendations for column connections detector settings and data processing considerations Tubing must have flat ends and must bottom out in the column endfitting Tubing must be connected to the column correctly to avoid creating a void between the column frit and tubing which can cause a leak and result in poor column performance
4. ed directly with 60 acetonitrile aqueous solution Flushing with 100 water after using the column around the pH limit might shorten the column lifetime Flush the column with water organic solution as described above such as 60 acetonitrile aqueous solution Once macromolecules such as proteins or polysacchandes are adsorbed onto the gel they are hardly removed even if solvents with high eluting capability are used 7 Other environments To prevent exposure of the column to excessive pressure the sample solution should be filtered through a 0 2 um membrane or smaller to renove particulates We recommend using a pre column filter to prevent the column fnt from being clogged with samples Mind for low sample loadability at operation in consideration of its small specific surface area about 80 of conventional fully porous silica based packing material in the same dimensional column The sample loadability is proportional to the specific surface area YMC CO LTD YMC Karasuma Gojo Bldg 284 Daigo cho Karasuma Nishiiru Gojo dori Shimogyo ku Kyoto 600 8106 Japan TEL 81 75 342 4515 FAX 81 75 342 4550 URL www ymc co jp E Mail sales ymc co jp UM140221AE 2 2
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