S 4245 UV/VIS DETECTOR USER MANUAL
Contents
1. Es nots ofon 29 30 7 INSTRUMENT MAINTENANG 7 1 Flowcell 7 1 1 Flowcell Assembly The following diagram shows all the components of the dismantled flow cell Element ol 000 31 7 2 Deuterium amp Tungsten Lamps Deuterium Lamp Tungsten Lamp Tungsten Lamp Conector Deuterium Lamp Connector Element 32 7 2 1 Lamp Exchange Ihe lamps can be accessed from the left side panel of the instrument e Remove the side panel to access the lamps Exchange Deuterium Lamp After opening the side panel the Deuterium Lamp can be accessed e remove the Deuterium Lamp by loosening the top and bottom screw 1 e disconnect the Deuterium Lamp 4 Now the Deuterium Lamp can be removed and a new lamp inserted Exchange Tungsten Lamp After opening the side panel the Tungsten Lamp can be accessed e remove the Tungsten Lamp by loosening the PEEK finger screw on top of the Tungsten Lamp housing 2 e disconnect the Tungsten Lamp 3 Now the Tungsten Lamp can be removed and replaced by a new lamp APPENDIX A SPECIFICATIONS A 1 Technical Specifications Wetted Materials Stainless Steel PEEK PTFE Quartz Baseline Noise 1x 10 AU 240nm 2 sec Risetime Baseline Drift lt 3x 10 AU h Wavelength Range 190 800 nm Wavelength Accuracy 2 nm Linearity gt 2 0 AU Light Source Deuterium Lamp Tungsten Lamp Wavelength P2. 2 The Rise Time is a electronic noise reduction where a higher value results in higher noise reduction Detector Lamps Select which lamps should be used for normal operation Multi Channel Mode Select the desired channel mode single channel or dual channel optional 5 3 2 Submenu Basic Settings gt Analog Output Ch1 Range Select the Range for Channel 1 The Range specifies the AU output scaled to a 1 V analog signal Ch2 Range Select the Range for Channel 2 The Range specifies the AU output scaled to al V analog signal optional Ch1 Offset Select an Offset for Channel 1 s analog signal Ch2 Offset Select an Offset for Channel 2 s analog signal optional Peak Detector Threshold Select the Peak Detection Threshold for detecting peaks If this threshold is reached a 24V output solenoid valve is activated to collect a fraction of this peak Peak Delay Select a delay for the Peak Detector This time delay should be equal to the time the sample needs to move from the detector s flowcell to the solenoid valve 23 24 5 3 3 Submenu Basic Settings gt Wavelength Program Action Time min 0 000 0 010 0 240 254 Al Al The step number of this row Time min The time when this wavelength program step activates A nm Select the new wavelength for this step Action Select between AZ OFF or None AZ execute an Autozero at the be
3. General Safety Information The operation of any analytical instrumentation requires the operator to be familiar with the potential hazards of using chemical solvents To avoid personal injury and or damage to the instrument the operator is responsible to follow all safety information herein The manufacturer assumes no liability for any damage resulted from not following any of these safety procedures 1 2 2 Intended Purpose This instrument is designed and certified as a general purpose laboratory instrument for research and routine analysis work only It is not certified for in vitro or other medical applications Any use outside this intended purpose does not fall with the manufactur er s liability 1 2 3 Environmental Safety Only operate the instrument in well ventilated areas If volatile or flam mable solvents are used with this instrument arrange for proper disposal of any waste and or fumes Always properly dispose any waste solvents Avoid open flames and sparks when working with flammable and volatile solvents In case of instrument leakage turn off the instrument and remedy the leak age problem immediately 1 2 4 Electrical Safety Always use the provided power cords Replace faulty power cords and other cables before operating the instru ment Always replace blown fuses with original spare fuses When the instruments housing is open electrical connections will be ex posed Disconnect the ins
4. S 4245 UV VIS DETECTOR USER MANUAL SS E Bla O 4245 UV Vis DETECTOR VERSION 1 0 2012 10 25 TABLE OF CONTENTS 5 3 3 Submenu Basic Settings gt Wavelength Program Contents 1 Introduction 1 1 How to Use this Manual 1 1 1 Symbols 1 2 Safety Information 1 2 1 General Safety Information 1 2 2 Intended Purpose 1 2 3 Environmental Safety 1 2 4 Electrical Safety 2 Instrument Overview 2 1 General Instrument Overview 3 Instrument Description 3 1 General Instrument Description 3 1 1 Front Panel Description 3 1 2 Back Panel Description 3 2 Functional Description 3 2 1 Front Elements 3 2 2 Functional Elements 3 2 3 Principle of Operation 4 Instrument Setup 4 1 Unpacking 4 2 Capillary Connections 4 3 Backside Connections 4 3 1 Electrical Connections 5 Instrument Menus 5 1 Keyboard 5 2 Status Screen 5 3 Menu Description 5 3 1 Submenu Basic Settings gt General 5 3 2 Submenu Basic Settings gt Analog Output Onanannnanuun wo 5 3 4 Submenu Deuterium Lamp Status 5 3 5 Submenu System 6 Instrument Operation 6 1 Overview 6 1 1 Internal Mode 6 1 2 External Mode 6 1 3 Serial Mode 6 2 Instrument Initialization Step 1 Status Init Step 2 Status Calib 6 3 Manual Operation Internal Mode 6 4 Remote Operation External Mode 7 Instrument Maintenance 7 1 Flowcell 7 1 1 Flowcell Assembly 7 2 Deuterium amp Tungsten Lamps 7 2 1 Lamp Exchange Ap
5. an abort the change of a parameter When pressing CANCEL key instead of EDIT key when changing a param eter the change is discarded and the original value is used CURSOR Keys The 4 arrow CURSOR keys are used to navigate the menus ZERO Key The ZERO key sets the baseline absorption value to zero 19 PROG Key Ihe PROG key starts the wavelength program 5 2 Status Screen Ihe Status Screen shows the most important parameters Mode Intern Status Calib Mode Intern Status Chl 20 Mode Ihe top line shows the currently selected mode Intern Extern or Serial Status On the top right the current status is displayed Init Calib Ch1 or Ch2 Wavelength Ihe currently selected wavelength Absorption Ihe current absorption at the selected wavelength 5 3 Menu Description With the MENU key the Main Menu is displayed Use the CURSOR key UP and DOWN to browse through the Main Menu for access of one of the Submenus E 21 22 5 3 1 Submenu Basic Settings gt General Chi Wavelength Select the desired wavelength for Channel 1 from 190 to 800 nm Ch2 Wavelength optional Select the desired wavelength for Channel 2 from 190 to 800 nm Ch1 Rise Time Select the desired Rise Time for Channel 1 The Rise Time is a electronic noise reduction where a higher value results in higher noise reduction Ch2 Rise Time Select the desired Rise Time for Channel
6. ginning of this step OFF calculate a baseline offset between the latest signal and ne new sig nal of this step None do nothing after changing the wavelength 5 3 4 Submenu Deuterium Lamp Status D2 Runtime Shows the total runtime of the deuterium lamp in hours D2 Total Ignitions Shows the total number of ignitions of the deuterium lamps since reset D2 Energy Shows the current lamp energy in 25 26 5 3 5 Submenu System Int Ext Ser Ihe Operation Mode can be changed here between Intern Extern or Serial Display Version Ihe Software Display is the installed firmware version of the Display PCB Mainboard Version Ihe Software Display is the installed firmware version of the Controller PCB GLP 24V The 24V Voltage is an actual voltage reading for one of the PCB s con trolled voltages GLP 5V The 5V Voltage is an actual voltage reading for one of the PCB controlled voltages 6 INSTRUMENT OPERATION 6 1 Overview The S 4245 UV Vis Detector can be operated in 3 different Operation Modes Internal External or Serial 6 1 1 Internal Mode In Internal Mode the instrument is controlled manually with the Keyboard 6 1 2 External Mode In External Mode the instrument is controlled via the Remote Digital I O contacts 6 1 3 Serial Mode In Serial Mode the instrument is controlled by a PC software e g Clarity via RS 232 serial line 6 2 Instrument Initiali
7. ith an optional second wave length This feature enhances the Wavelength Program feature that you can measure 2 different wavelengths at the same time A second D A converter output comes with this option to keep the system flexible to be used with any data acquisition software available Optional Online Scan Another option for the S 4245 UV Vis Detector is the Online Scan With the Online Scan whole spectrum information can be gathered at a certain time This scan information is stored internally and can be accessed at any time The Online Scan is a good alternative to a full UV PDA detector Figure S 4245 Optical Module 3 INSTRUMENT DESCRIPTION 3 1 General Instrument Description 3 1 1 Front Panel Description The front panel of the S 4245 UV Vis Detector consists of three main ele ments the TFT Display 1 the Keyboard 2 and the Front Cover 3 The 3 TFT Display shows the current status information and is used with the Keyboard to adjust instrument settings and fully operate the instru ment in stand alone mode without a PC required The Front Cover has a view panel so that the important parts are always visible to the operator In case of a leak it can be detected before solvents are leaking out of the bottom of the Front Cover The Front Cover can be opened to easily access the flowcell S 4245 UV Vis DETECTOR Element 11 Removing the Front Cover The Front Cove
8. nect the drain capillary to the flowcell outlet 3 S 4245 UV Vis DETECTOR Element 17 4 3 Backside Connections 4 3 1 Electrical Connections e Connect the supplied power cord to the Mains connector 2 e Connect the supplied RS 232 cable to the RS 232 connector 4 if the instrument is to be operated by PC e Connect the remote control wires to the Digital I O connector 3 if the instrument is to be run in External mode Please refer to 5 Instrument Operation for further information about PC control and Remote control Element Serial Number JF 7 O Remote Digital I O L y Er MAINS 4 RS 232 Serial Communication 50 60 Hz POWER 100 240 V 5 RS 485 Communication Bus GE ANa USB Communication not in use SB RS 485 RS 232 REMOTE E 18 5 INSTRUMENT MENUS 5 1 Keyboard The S 4245 UV Vis Detector parameters can be adjusted at any time via the screen menu and keyboard on the front panel nor SO MENU Key With the MENU key you can access the Main Menu and select a sub menu STATUS Key With the STATUS key you can access the status screen from any menu EDIT Key Use the EDIT key to change any parameters When you press the EDIT key the selected parameter can be changed Confirm the new setting by pressing the EDIT key again CANCEL Key With the CANCEL key you c
9. onal elements of the S 4245 UV Vis Detector are located inside the instrument The Display PCB 1 controls the TFT Display and Keyboard It is acces sible when the display panel is removed The Lamp Power Supply Unit 2 supplies the required voltage and ignition sequence for the Deuterium lamp The Power Supply Unit 3 supplies 24V to the PCB s The input voltage is variable from 90 V to 240 V 5 The Controlled PCB 4 controls the whole pump The Fan 6 is mounted on the back panel and is important for regulating the internal temperature The Lamp Connector Rack 7 is used for easy connection of Deuterium and Tungsten lamps from the side panel of the instrument Element ee a optical Module 15 3 2 3 Principle of Operation Reference Signal 2nd Order Filter Deuterium Lamp Tungsten Lamp Figure Principle of Operation 16 Measuring Signal 4 INSTRUMENT SETUP 4 1 Unpacking Remove the S 4245 UV Vis Detector from its package and put it on the working desk Check the instrument thoroughly for any damage that may have occurred during shipping Contact your supplier in case of any dam ages Check the accessories shipped with the instrument if everything is com plete and in good condition 4 2 Capillary Connections Connect the capillary coming from the separation column to the flowcell inlet 4 Then con
10. pendix A Specifications A 1 Technical Specifications A 2 Environmental Conditions A 2 1 Operational Conditions A 2 2 Storage Conditions Appendix B Accessories B 1 Standard Accessories Appendix C Version Control C 1 Version History 24 25 26 27 27 27 27 27 27 27 27 28 29 31 31 31 32 32 33 33 34 34 34 35 35 37 37 1 INTRODUCTION 1 1 How to Use this Manual This manual is designed as a reference to the installation operation and maintenance of the S 4245 UV Vis Detector It is strongly recommended to review this manual before operating the in strument The content of this manual is subject to change without notice This docu ment is believed to be complete and accurate at the time of publication The Manufacturer is not liable for any damage resulted from the use of this manual 1 1 1 Symbols Throughout this manual important text sections are marked with the fol lowing symbols This section includes important information for the proper opera tion of the instrument Failure to follow this information may result in faulty behaviour of the instrument and or wrong analysis results This section emphasis some detailed information intended to optimize the performance of the instrument or to give a better understanding of some technical details 1 2 Safety Information This instrument is compliant with all related standards as stated in Ap pendix B 1 2 1
11. r of the S 4245 UV Vis Detector is completely removable at its hinges Open The Front Cover to 90 in order to get free of any instrument stacked on top e Remove the Front Cover e Store the Front Cover at a safe place 12 3 1 2 Back Panel Description The back panel of the S 4245 UV Vis Detector houses the power and inter face connectors and several drain outlets The Power Cord Connection amp Power Switch housing holds the main fuse in an internal fuse carrier The digital I O and other interface connectors are used for remote instru ment control and are discussed in a later chapter Element Power Switch 5 RS 485 Interface Connectors USB Interface Connector S N MAINS 50 60 Hz POWER 100 240 V FUSE 2 0AT S el 999 SB RS 485 RS 232 REMOTE 13 3 2 Functional Description Element e Fioweel outs 14 3 2 1 Front Elements The S 4245 UV Vis Detector has all important parts directly accessible from the front panel when the Front Cover is opened or removed The Capillary Holder 3 holds the pump inlet capillary in place The flowcell 4 is completely accessible from the front to make capillary connections to the flwcell inlet 5 and outlet 6 and can be easily removed with 2 screws 01898 PE S 4245 UV Vis DETECTOR 3 2 2 Functional Elements All functi
12. rogram Programmable 10 steps Analog Output 1x 1 V optional 2x 1 V Control Features Internal Peak Detector with 24V solenoid switching output Dimensions 310x 165 x 478 mm WxHxD Weight 7 0 kg Power Supply 100 250 V 47 63 Hz depending on material option 33 A 2 Environmental Conditions A 2 1 Operational Conditions Ambient Temperature l02 702 3570 Ambient Relative Humidity 20 to 80 RH non condensing A 2 2 Storage Conditions Ambient Temperature 20 C to 60 C Ambient Relative Humidity 20 to 80 RH non condensing 34 APPENDIX B ACCESSORIES B 1 Standard Accessories The S 4245 UV Vis Detector is delivered with the following standard ac cessories e 1x Power Cord EU Type e lx Serial Cable RS 232 e 1x 1 16 Drain Capillary FEP I D 0 8mm 2 m e 1x 1 16 Fitting amp Ferrule Stainless Steel e 1x Operation Manual this e 2x Fuse 2 A 36 APPENDIX C VERSION CONTE C 1 Version History The 4245 UV Vis Detector Operation Manual is subject to version control The version history is continued and noted on every document The access to the original document is restricted to the creating party The document is subject to periodical checks and can never reach an unchangeable state Version Release Date Description 1 0 2012 12 03 First Release 37
13. trument from the main power before opening the housing The housing should only be opened by certified service personnel Damage of the instrument of injury may result from improper han dling 2 INSTRUMENT OVERVIEW 2 1 General Instrument Overview Ihe S 4245 UV Vis Detector is a variable wavelength UV Vis detector for routine analysis and sophisticated research The dual lamp design offers a wavelength range of 190 800 nm with a low baseline noise The front accessible flowcell can easily be exchanged as can be the lamps which are accessible through a side panel in the instrument housing Integrated Wavelength Program The S 4245 UV Vis Detector features a wavelength program to change the selected wavelength over time With this feature the optimum wavelength can be selected for each analyzed substance according to its retention time Integrated Peak Detector The integrated Peak Detector works as a basic fraction collector The peak detection level can be freely programmed for peak start and peak end to enhance the collection purity An integrated 24V output for switching a solenoid valve is used for the fraction collection which is automatically operated with a selectable time delay Peak Start Detection Level Peak Start Peak End Time Solenoid 3 0 Valve OFF Fraction Collection Valve ON Figure S 4245 Peak Detector 10 Optional Dual Wavelength The S 4245 UV Vis Detector is available w
14. zation When the S 4245 UV Vis Detector is switched on it goes through a number of initialization steps These steps are outlined below Step 1 Status Init The detector measures its dark current and check the lamps Step 2 Status Calib After the initialization the detector calibrates its wavelength This takes some time as the grating has to be moved through the whole spectrum to calibrate itself 27 6 3 Manual Operation Internal Mode 28 The S 4245 UV Vis Detector can be operated manually When the instrument is in Internal mode the detector measures continu ously 6 3 1 Autozero Press ZERO key on the front panel to execute autozero 6 3 2 Wavelength Program Press PROG to start the wavelength program if the program key is cur rently assigned to the wavelength program 6 3 3 Online Scan Press PROG to start the online scan if the program key is currently as signed to the online scan The scan replay is also started with the PROG key if assigned to the replay function 6 4 Remote Operation External Mode Some functions of the S 4245 UV Vis Detector can be operated exter nally Please refer to the Remote Connector layout on the right NOTE Pin 1 of the connector directly faces the RS 232 connector The REMOTE START function corresponds to the Start key on the front panel and can either start the wavelength program or online scan REMOTE Pin Function 6 Error Output
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