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uProcess User Manual

1. lt e Dock at Bottom j Sequencer seas Figure 30 Positioning Guides for Re docking a Window 65 APPENDIX B MAINTENANCE Updating Firmware The firmware for all uProcess devices can be field updated as follows Download the latest version of uProcess software from www labsmith com Choose Actions gt Firmware gt Update All Firmware uProcess will upload the latest firmware associated with the installed software to all connected syringe pumps 4VM0O1 valve manifolds and 4AM01 analog manifolds Devices that are not attached will not be updated Note AV200 series automated valves and uPS01 series pressure sensors do not have firmware and therefore do not need to be connected for firmware updates Note You can update the firmware for a particular device by clicking the Configuration button in the device control panel uProcess devices are factory configured for optimal performance From time to time it may be necessary to access and adjust these settings Valve Actuation Profile Settings Click the Configuration button to open the Configure Device dialog box shown in Figure 31 The Actuation profile settings control the motor actuation duration and current These settings may need to be adjusted as your valves and motors age However the user should contact LabSmith before changing any of the Actuation Profile Settings as incorrect settings can cause excessive wear of the motor or prevent the motor from reach
2. PI uProcess Reagent Syringe SPSO1 20 ul Reagent Manifold 4VM01 Open_Reservoir Reagent Manifold SetValves 0001 ovis Interf Reagent Syringe Stop G1 LabSmith EIB200 on COM44 lt Wait llDone gt Reagent Manifold Addr 11 Fill_Syringe Reagent Syringe SetFlowRate 50 000 ul min Reagent Syringe MoveTo 10 000 ul Reagent Manifold Stop lt WaitAllDone gt Sequencing Script i Reagent Syringe SPS01 20 ul f Reagent Manifold 4VM01 E Open_Reservoir E Reagent Manifold SetValves0001 E Reagent Syringe Stop H lt WaitAllDone gt Reagent Syringe MoveTo 10 000 ul B Reagent Manifold Stop ff lt WaitAllDone gt 15 39 47 363 Parsing script 15 39 47 363 Parsing succeeded with no errors in 16 lines 15 39 47 363 All objects declared in the script are connected 15 39 47 363 The script is ready to run 7171 Script Parsing Dan ed Figure 31 Step 1 Shown in the Sequence Tab and Main Window 45 Set the position of Valve 1 to Open B and depress the button for the Reagent Syringe Click the Save As a New Sequence Step button and name the new step Open_Process Choose Pause and set the time for 1 s 1000 ms Choose Continue and select OK to go on to the next step Set the Target Volume to 0 and the Flow Rate to 10ul min and depress the button for the Reagent Manifold Click the Save as a New Sequence Step button Name the step Deliver Reagent select Wait until all devic
3. Pump Properties Address 74 Syringe Size Standard 80 ul Online Monitor oyi Mge sIZe Speed Control mas 100 000 ul min 0 073 Position Monitoring amp Control Z ul F set motion via Ch Set Point J 60 000 ul 104 ul 0 0 ul 80 000 ul scoot C E Figure 19 Additional syringe pump control options available in the Syringe Pump uDevice Control Panel Target Volume is set by dragging the volume slider or entering the desired volume in the Target volume box Flow Rate is set by dragging the flow rate slider or entering the desired rate in the Flow rate box Note If Update All uDevices Continuously IS is selected changes in target volume or flow rate are immediately applied If not selected the pump settings will not change until Apply All Settings Now or is selected The Motion Indicator shows the pump motion 26 green indicates that the pump is pushing red indicates that pump is pulling K G ae i grey indicates the pump is stationary ja C a red X next to the syringe tip indicates the pump is stalled The syringe pump can be used to regulate pressure by clicking on the Set motion via Ch until the desired Channel setting is selected The Channel Setting options are Move with Ch X Syringe pump will push when pressure on Ch X is below the desired setting Syringe pump will pull when pressure on Ch X is above the desired setting Move opp Ch X Syringe pump
4. P LabSmith SP501 Addr 53 lem EIB on COMI 3 4 valve Manifold Addr 01 P LabSmith 5P501 addr 108 Figure 11 Devices Shown Offline EIB on Com11 and Online EIB on COM1 Device Naming and Numbering Every uDevice syringe pump valve manifold or analog manifold is identified by an address and name The uPS pressure sensors also are individually named Note Clicking on the device name in the uDevices Window will cause the indicator light on the device to flash red green This feature aids in quickly identifying each device Device Address Each device attached to an EIB must be assigned a unique address between 0 and 110 If you purchased several devices at the same time from LabSmith they generally will be preconfigured with unique addresses Device addresses are shown next to each device in the uDevices Window and also in the Address field of the Individual Device Control Panels Note If multiple devices on the same EIB have identical addresses they may not appear in the uDevices Window list or they may appear as one device Operating in this condition can cause unpredictable results If two devices with the same address are installed one will need to be removed before the address can be changed 16 Note The address assigned to each device is independent of its physical position on a breadboard Devices will be displayed in numerical order on the control panel for each EIB based on the device address not the position
5. Click the Calibrate button Select OK to store the values Tip To obtain the maximum volume of the syringe pump Remove the syringe glass from the SPS01 but leave in the plunger and run the calibration Then replace the syringe glass carefully pushing the syringe glass back until it hits the end of the plunger Rerun the calibration after tightening the syringe glass in place Care of the Syringe Glass and Plunger Plunger Care Do not apply excessive force to move a plunger Too much pressure can irretrievably bend the plunger or easily crack the syringe s glass barrel Avoid touching the plunger with your fingers Abrasions scratches or oil due to handling the plunger with your fingers may interfere with proper plunger operation To minimize the chance of damage or contamination do not store the plunger outside the glass Use caution when reinserting the plunger into the barrel using the plunger installation tool For best results dip the PTFE plunger tip into your solvent to re wet it prior to reinserting the plunger into the barrel Cleaning Sterilizing Autoclaving and Disinfecting Cleaning To clean syringes it is best to use solvents known to be effective in solvating the sample Preferred cleaning agents are non alkaline non phosphate and non detergent based A biodegradable non phosphate organic Cleaning Concentrate is available from Hamilton Company Part No 18311 www hamiltoncompany com 69 Rinse the
6. in a script WaitDone ends when the reading is in range Figure 15 Regulation Setting Window The following options are available for the Regulation Settings Window Target reading input target pressure value in specified units e Check too low the sensor will regulate up to the input value e Check too high the sensor will regulate down to the input value when the actual pressure is equal to or below the target reading the regulation will be complete Tolerance input tolerance for target reading e the sensor will regulate until the pressure value is within the target tolerance e Hysteresis checking this option causes the pressure to regulate to the extreme of the target tolerance and then the regulation stops until the pressure drifts to the opposite tolerance level Note the tolerance and hysteresis options are only activated if both check too low and check too high are selected Channel Regulation check the box and select the channel to send the regulation status to an EIB hardware channel Channel regulation can be used to couple the pressure sensor to a SPS01 syringe pump or automated valve to actuate the syringe or valve based on the pressure regulation status 22 lf a hardware channel is selected the channel status will be shown on the EIB control window and the analog sensor panel Regulation Status Indicators include T Regulating Up w Regulation satisfied ka Regulating down Unreg
7. Calibration from File Sensor serial number uPSO12 000 0131 Figure 17 Sensor Properties Window Note adjusting the zero offset from this Sensor Properties window will preserve the original factory calibration The sensor offset can also be adjust from the main menu at Action gt Sensor Calibration gt Tare Set Current Reading Adjusting the offset from this menu will change the calibration file data The Sensor Properties Window provides the following additional controls Sensor Name Display Units Display limits Calibration Factory Settings the default name is the last 5 numbers of the Sensor serial number default units are kPa a conversion factor can be input to display the pressure in units other than kPa these are the limits displayed in the Control Overview Window and the Analog Manifold Control Panel the pressure sensors are shipped with a factory calibration please contact LabSmith before changing the calibration please contact LabSmith before changing the factory settings Syringe Pump Control Syringe pump control of the set volume and flow rate and the pressure regulation settings can be manipulated via the Device Control Overview Window as shown in Figure 18 The Syringe Pump uDevice Control Panel Figure 19 provides additional position control options and Configuration settings 25 Target volume Flow rate 60 000 ul 85 355 ul 100 000 Els 0 0 X 0 073 et motion via Ch Dye
8. Click the Online toolbar button to toggle all interfaces back online Click the Rescan toolbar button E to check for new devices Any added devices should now appear in the uDevices Window while any removed devices will be gone Note To rescan an individual EIB right click its icon in the uDevices Window then choose Rescan Devices 17 Saving Data into a Log File Time resolved data can be saved into a data file format csv for later viewing and plotting Choose File gt Log Status Measurements to create a Log File When log file data is being collected there will be a check mark next to the Log Status Measurements item in the manu The file will continue to collect data until you uncheck the box close the software or change any of the uDevices add or remove devices External Instruments The uProcess software supports several third party instruments These instruments are connected to the computer via USB or RS 232 connection and depending on the instrument can be monitored and or controlled Contact LabSmith support Support labsmith com for information and instructions on supported instruments 18 CHAPTER 4 REAL TIME DEVICE CONTROL uProcess lets you control connected devices manually or as part of an automated process uDevice control can be accessed two ways e through the Control Overview Window accessed by clicking on the Interfaces heading in the uDevices window e or through an individual Device
9. Open B Set via Ch Set via Ch Target volume Flow rate amp Sequencing Script ul 0 882 ul ul min ul min J sK 9 20 ul Set motion via Ch 0 0 0 0145 560 LabSmith 4AM 11586 1 51 kPa Reg 11587 mns 1 66 kPa _ Reg fo sensor 3 Mo sensor 4 Ensure the Update all uDevices Continuously button B is NOT selected If selected press the stop button to unselect x Select the action for the first step For example turn valve 1 to A and valve 2 to Closed LabSmith 4VM Valve 1 Valve 2 No valve 3 No valve 4 m Open A Open A Close i Close E Open B E Open B Set via Ch Set via Ch Click the Save as New Sequence Step button JR The Creating a New Sequence Step dialog box will open Figure 23 31 Creating a new sequence step K bnn New step stepl After changing the uDevice settings Pause 4000 ms Wait until all devices are finished l Do not wait then Continue End if this is the last step C Loop to step T 0 times 0 ea Figure 23 Creating a New Process Step Dialog Box If desired enter a descriptive Step Name Step names may have a maximum of 16 characters and may not include blanks or lt gt or Select how the script should respond after changing the uDevice settings Select Pause then enter the duration to pause before continuing with the process The pause duration begins as soon a
10. Step2 _Fill_Syringes iteration 1 Talal Script Parsing Sequence Statu 00 00 04 493 Run the current sequence of steps iteration 1 bolls Figure 6 Output Window Showing Script Parsing Tab The Output Window contains three tabs Script Parsing displays any errors in the current automated process Sequence Status indicates which process is running and notes any errors incurred Communications shows the status and history of communication between the Electronic Interface Board and the computer Main Window The Main Window is used to control and monitor devices as well as to construct edit and monitor sequence programs Its contents change depending upon what is selected in the uDevices Window or Sequencer Window The Main Window cannot be undocked or hidden Communicating with the uDevices Once the uDevice hardware and uProcess software have been installed you are ready to communicate with the devices Launch the uProcess software Right click on Interfaces in the uDevices Window and choose New Interface Select the computer port to which the EIB is connected and click OK If multiple EIBs are installed repeat this step for each EIB Your uProcess dashboard should now look similar to Figure 7 12 uDevices oes o os iB LabSmith EIB01 on COM6 Water Addr 50 EESE Dye Addr 55 EE fl LabSmith 4VM Addr 63 ch a E chs E ch c E cho E 75 LabSmith EIBO1 on COMS LabSmit
11. Syringe Addr 35 af FO Instruments 2S Sequencing Script E Reagent Syringe SPSO1 20 ul K Reagent Manifold Reagent Manifold E Open_Reservoir ffi Reagent Manifold Stop E Reagent Syringe Stop ff lt WaitAllDone gt Sequence idle LabSmith EIB200 ch Aa 2 Reagent Manifold No valve 1 Valve 4 Open A Close Open B Set via Ch Reagent Syringe Target volume Flow rate 99 10 000 yl 11 740 yl 50 000 ul min Ss gt 20 ul Set motion via Ch 0 0 15 21 25 985 Parsing script 15 21 25 985 Error on line 2 Unknown uDevice type Reagent Manifold 15 21 25 985 Parsing failed with 1 error in 7 lines 15 21 25 995 All objects declared in the script are connected Click the Save As a New Sequence Step button Name the new step EiL Syringe 44 Choose Wait until all devices are finished and Continue Creating a new sequence step quae After changing the uDevice settings Pause 4000 ms Wait until all devices are finished Do not wait then Continue End if this is the last step Loop to step Open_Reservoir 0 times 0 Figure 30 Step 1 Settings Click OK to add this step to the script To view the process thus far click the Sequencer Window tab then click on any step in the sequencing script Select Interfaces in the uDevices Window to return to the Device Control Overview Window and continue building the script
12. This Manual Several conventions are used throughout this manual to highlight important features and concepis Menu gt Option Menu bar selections will be shown in bold text with descending options separated by the gt symbol For example Help gt About uProcess indicates that you should click the Help menu and then click About uProcess Note Notes indicate helpful tips or additional information CAUTION These notes signify important considerations that will help you to avoid losing data or damaging hardware Italics When browsing the electronic version of this manual click on these italic cross references to view other pertinent sections of the manual Choose Help gt About uProcess then click the Open User Manual link to browse the electronic version of this manual Please contact LabSmith or visit www labsmith com for user manual software and firmware updates CHAPTER 2 GETTING STARTED Connecting an Electronic Interface Board Though the devices you use with your uProcess system will vary some basic hardware is common to all uProcess setups Every system requires at least one uProcess Interface EIB200 which can control up to 110 SPS01 Syringe Pumps 4VM01 Valve Manifolds and or 4AM01 Analog Manifolds Since each manifold can control up to four devices one EIB200 can thereby control hundreds of devices Although over 100 devices can be sequentially controlled using a single EIB200 the number of devices simulane
13. already in process for that use the Stop function Example lt Quit gt Note The Quit function cannot be added using the Process Wizard Hed Down To and RegUpTo SensorName Red Down To value units regulation is complete when sensor pressure reaches or drops below input value or SensorName RegUpTo value units regulation is complete when sensor pressure reaches or exceeds input value SensorName defined name of sensor must be defined in script header value pressure positive or negative number units pressure units acceptable units kPa Example P032 RegDownTo 50 kPa 37 RegDownTo Channel or RegUpTo Channel Similar to RegDownTo and RegUpTo except it sends the output value to a specified channel which is used to control one or more SPS01 syringe pump Requires two lines of code SensorName RegDownTo value units to Chx SyringeName MoveWith ChxX or SyringeName MoveOpposite Chx Or SensorName RegDownTo value units to Chx SyringeName MoveWith ChX or SyringeName MoveOpposite Chx SensorName defined name of sensor must be defined in script header value pressure positive or negative number units pressure units acceptable units kPa SyringeName defined name of syringe pump must be defined in script header channel A B C or D MoveWith Chx pulls the syringe to decrease the pressure pushes the syringe to increase the pressure MoveOpposite ChX pushes the syringe to decrease the pressure pulls th
14. on the breadboard To change a device address Select the device in the uDevices Window Choose Configuration Select a Device Address from the dropdown menu and click OK You can also right click on the device icon in the uDevices Window and choose Change Address Device Name Your uDevices will arrive with a default name LabSmith SPS01 for the syringe pump LabSmith 4VM for the 4VM01 and LabSmith 4AM for the 4AM01 You can rename the devices The name may have a maximum of 16 characters and may not include lt gt or Names may include tabs and spaces but may not start or end with them Names are case sensitive If you will be using automated processes each installed device name must be unique To change a device name Select the device in the uDevices Window Choose Configuration Type the new device name in the Name field You can also right click on the device icon in the uDevices Window and choose Rename NOTE Each pressure sensor arrives with the last five numbers of the serial number as the sensor name The pressure sensor name can only be changed within the 4AM configuration tab Please see the Analog Manifold Control section of Chapter 4 for information on changing the sensor name Adding or Removing uDevices To add or remove a device Click the Online toolbar button G to toggle all interfaces offline Physically add or remove the uDevice s to or from the breadboard
15. out of the syringe body being careful not to bend the syringe plunger 8 While holding the wide end of the plunger installation wrench insert the wrench around the plunger tip and into the syringe until it engages with the hex head at the base of the syringe plunger vj GE Pump 67 CAUTION Be very careful not to damage Teflon end of the plunger Damage to the plunger can cause the syringe to leak 9 Turn the wrench counterclockwise approximately two revolutions to unscrew the plunger 10 Gently pull the wrench and plunger out of the syringe CAUTION Do not operate the syringe without a plunger installed Doing so can damage the syringe To install the syringe glass and plunger 1 While holding the wide end of the plunger installation wrench insert the plunger assembly into the wrench such that the hexagonal feature on the plunger is engaged with the wrench 2 Gently insert the plunger assembly into the syringe and screw onto the shaft until hand tight approximately two revolutions 3 Carefully press the tapered glass syringe over the plunger assembly and into the syringe until it is seated in the syringe body Use the hex wrench to tighten the screw located on the top side of the syringe Gently pull on the syringe glass to ensure it is secure in the syringe body 6 The glass size value must be input in the software each time you change the glass size to allow uProcess to display and compute correct volumes an
16. script header Example uPS1 IfDone i E Note The lfDone function cannot be added using the Process Wizard lfNotDone DeviceName lfNotDone Log If regulation is not done the immediately following step will be executed otherwise the immediately following step will be skipped DeviceName_ defined name of uPS pressure sensor must be defined in script header Example uPS1 IfNotDone lt goto gt Stepl Note The lfNotDone function cannot be added using the Process Wizard lt log gt on optional filename lt log gt off Opens closes a log file filename file path and name see examples if omitted file naming will be as specified in View gt Status Meas Logging Options Note if the file name or path contains a space the entire name and path must be surrounded by double quotes see example Note a Scrip Error will occur and the script will halt if a lt log gt on command is issued when a log file is already open or a lt log gt off command is issued when a log file is not open 35 Examples lt log gt o lt log gt on C Temp testlog csv lt log gt on C Temp test log csv Loop lt Loop gt SteopName cycles Loops back to named step for specified number of cycles SteoName defined name of step in script cycles number of times to loop Example lt Loop gt FillStep 6 Note For an endless loop use the lt Goto gt command MoveTo SyringeName MoveTo vol units Sets the desired syringe position SyringeN
17. step can change the state of multiple devices A script can be created using the Process Wizard and it can be created or edited using the built in text editor Opening Script Files Each automated script is stored as a file with a usq extension To open an existing script click the Open toolbar button then browse for the correct file Its contents will appear in the Main Window Figure 22 ShReagent Syringe SPS01 80 ul Valve Manifold 1 4V M01 Fill Syringe Valve Manifold 1 SetYValves 0001 Reagent Syringe SetFlowRate 50 000 ulmin Reagent Syringe MoveTo 100 ul lt WaitAllDone gt Deliver _Reagent Valve Manifold 1 SetYalves 0 0 0 3 Reagent Syringe SetFlowRate 10 000 ul min Reagent Syringe MoveTo 0 000 ul lt WaitAllDone gt Figure 22 Text of an Automated Process Create a Script Using the Wizard The uProcess Wizard provides a simplified interface for quickly generating automated scripts Note Each installed syringe pump and 4VM01 must have a unique name regardless of whether it is used in an automated script See Device Name To create a script using the uProcess Wizard 1 Click Interfaces to view the Control Overview Window 30 File Edit View Actions Help S 9 S Interfaces _ 795 LabSmith E1801 on COMS LabSmith EIB01 ganian Ch A e chp 7 che J cho lt A LabSmith 4VM Valve 1 Valve 2 No valve 3 No valve 4 Open A Open A Close F Close Open B
18. uDevice Contact LabSmith about obtaining a custom communication cable if connecting multiple uDevices without a breadboard The larger breadboards uPB 8 uPB 12 and iBB can be daisy chained together by installing a 20 pin communication cable between female connectors on each board Figure 2 uPB 05 and uPB 08 Breadboards with uDevices The following sections provide information on installing the various devices available for the uProcess system Note disconnect power to the EIB200 before adding or removing devices Installing Automated Valves To install and configure AV201 or AV202 automated valves e Plug the 4VM01 valve manifold into one of the connectors located along the side of the breadboard e Secure the automated valve s to the desired location on the breadboard using two 4 breadboard mounting screws optional e Use a ribbon cable to connect the automated valve to a port on the valve manifold Observe the label on the valve and manifold to ensure the cable is connected at the correct orientation e Upon power up the LED on the 4VM01 will flash red green and then glow steady green The valve LED does not illuminate during power up Installing Syringe Pumps To install an SPSO1 programmable syringe pump plug the syringe pump body into one of the connectors located along the side of the breadboard Two M3 screws may optionally be used to more securely attach the syringe to the bottom of the breadboard Upon powe
19. will pull when pressure on Ch X is below the desired setting Syringe pump will push when pressure on Ch X is above the desired setting Note Clicking on the target volume input box or moving the volume slider will disable the pressure regulation See Analog Manifold Settings for more information on regulating a uPS pressure sensor via a channel setting Optimizing Syringe Pump Performance The syringe pump is designed for optimal accuracy in the push infuse direction The pump motor design includes an anti backlash spring which can cause minor volume inaccuracy in the pull direction In addition when changing the syringe direction from pull to push or vice versa you may notice a slight delay before the syringe plunger starts advancing The volume inaccuracy and duration of the delay will vary depending on the syringe size set speed system back pressure and fluid viscosity To improve the timing and volume accuracy of an infusion we recommend pushing a small volume of fluid prior to starting the timed infusion For example if you want to pull 10 ul of fluid from a reservoir and then push infuse it into a process we recommend pulling 11 ul of fluid then push 1ul back into the reservoir before switching your valve to the infuse position Valve Manifold Control Click a valve manifold in the uDevices Window to view its control panel Figure 20 27 Valve 1 Valve 2 Valve 3 Valve 4 No change No change A OA 8
20. 1 Closed Closed B B With a 9 cma Q a che lt bernia In Sana LabSmith 4VM Valve 1 Valve 2 No valve 3 No valve 4 T Open A Open A Close Close v Open B Fl Open B Set via Ch IW With Ch c Figure 20 Controls for a 4VM01 valve manifold with AV201 or AV202 automated valves The valve can be actuated to three positions see Figure 21 as shown below No change The valve state will not change indicated as position 0 in script A The valve will allow flow to the left indicated as position 1 in script Closed The valve will close indicated as position 2 in script B The valve will open flow to the right indicated as position 3 in script VOO OOO Closed 2 Closed 2 Avan Series 3 port T AV202 Series 4 port ANE Figure 21 Shows the valve flow positions looking down from the front of the valve The indicator lights on the valve will illuminate when it is being actuated A red light indicates that it is being actuated to the A position while a green light indicates it is being actuated to the B position lf the current valve state is known there will be a check next to the state and the message below the valve will read In position lf the current valve state is unknown i e if power to the EIB has been cycled none of the check boxes will be marked and the message below the valve will read Indeterminate NOTE If valve cables are switched while the E
21. Control Panel which can be accessed by clicking on the device name in the uDevices window EA uProcess i OU a d 4 sB aee K u File Edit View Actions Help uDevices Click to access Control Overview Window EMU Interfaces 4 65 EIB on COM6 The Control Overview Window gives you a convenient way to view and set the controls for multiple devices which is particularly useful when setting up the steps of an automated process or when controlling multiple devices via pressure regulation Figure 12 shows a typical Control Overview Window The individual Device Control Panels can be selected for additional controls for the particular devices 19 F uProcess DEH yae eO KL 04102006 File Edit View Actions Help uDevices aX c s x Interfaces Ha 5 EIB on COM6 A LabSmith 4VM Addr 03 E LabSmith EIB01 LabSmith 4AM Addr 67 S Dye Addr 74 Cha amp ch B lt Gh C e Ch D f Defaults A Water Addr 77 E Instruments LabSmith 4VM R Valve 1 Valve 2 No valve 3 No valve 4 Open A Open A _ Close Close I _ Open B Open B _ Set via Ch Set via Ch i LabSmith 4AM x 0041 0 789 kPa Reg E R 073X mi 24 834 kPa Reg E 066S Y 15 976 kPa Reg E 1 R 072x U 1 261 kPa Reg E M Dye P Target volume Flow rate L ul 0 175 ul ul min R g T g 4 104 ul
22. ETTING STARTED Connecting an Electronic Interface Board Adding a Breadboard Installing Automated Valves Installing Syringe Pumps Installing Pressure Sensors Installing uProcess Software CHAPTER 3 uPROCESS SOFTWARE Software Interface Communicating with the uDevices Online and Offline Device Naming and Numbering Saving Data into a Log File External Instruments CHAPTER 4 REAL TIME DEVICE CONTROL uDevice Controls CHAPTER 5 AUTOMATED CONTROL Opening Script Files Create a Script Using the Wizard Save a Script File Run Pause and Quit a Script Automated Script Functions Editing Automated Script Text Example Script Syringe Pump Volume vs Delivery Time Pressure Regulation Example Scripts CHAPTER 6 SVM340 AUTOMATION AND CONTROL APPENDIX A MENUS TOOLBARS WINDOW CONTROLS APPENDIX B MAINTENANCE Updating Firmware Valve Actuation Profile Settings Changing the Syringe Pump Glass Assembly Calibrating the Syringe Pump Care of the Syringe Glass and Plunger APPENDIX C uDEVICE CURRENT CONSUMPTION APPENDIX D CAPILLARY AND TUBING INSTALLATION GUIDE CO COON N VN ODD UV A a PPP ka ka ka O0 O G Ch N amp WN Pe O O WO Q BB WWW W W w OON UO ANN G GC a a ui a WwW a oan a ad A O COON A OA NOON N e CHAPTER 1 INTRODUCTION LabSmith s uProcess is a platform comprised of a suite of hardware and software products that simplify construction and control of microfluidic systems uProce
23. Finish 1 os Check Loop Pinched Flow Example Script with Pressure Regulation The uProcess system can also be used to create near constant pressure flow as shown in the following pinched flow application example Fluorescent dye is injected in the main leg of a cross chip and water is injected in the two cross arms to create pinched flow across the width of the channel A reservoir BB_RES out fitted with an o ring and cap is placed upstream of each chip inlet Each reservoir is partially filled with the corresponding liquid dye or water leaving an air gap at the top to allow flow dampening A uPS pressure sensor is installed in the cap of the reservoir to monitor and regulate the pressure 52 Valve 1 SOG iy T Y interconnect Water 80 7 3 splitter reservoir with uPS 2 Pressure regulated hydrodynamic flow application iBB breadboard 4VM manifold and 4AM manifold not shown for clarity The script below will 1 Switch_Fill open the valves to the open reservoirs 2 Fill fill the syringes 3 Switch_Dispense open the valves to the chip 53 4 Regulate dispense the syringe fluids toward the chip DYE a Dye_80 syringe dispenses until reservoir_1 pressure reaches 46 kPa b Dye 80 syringe stops pressure monitored until pressure drops below 45 kPa c water dispense until reservoir_2 pressure reaches 37 5 kPa PUES a uPSO1 vE A uPSO1 x Dye 00S SPSOL Water 80 SPSO1 4AM 4AMO1 x4VM AVMO1 L bat
24. IB is powered the valve states could be incorrectly indicated Remove power to the EIB before changing valves or moving cables lf Update Continuously is selected the valve will move immediately when you select a new position and the check mark to the right of that position will be marked When Update Continuously is not selected choose the new position then click Apply Settings Now to move the valve the check mark for the new position will be then marked The valve can also be instructed to set based on channel regulation status 28 No change A 1 Dun Valve 1 If Open A Close Open B With Ch A or Set via Ch The valve will change states based on the regulation status see Chapter 5 If the valve is selected to move With the channel the default valve positions are 4 Regulating up pressure is too low valve will move to position B Regulation achieved pressure is within range valve will move to position Closed Z Regulating down pressure is too high valve will move to position A lf the valve is selected to move Opposite the channel the default valve positions are 4 Regulating up valve will move to position A 7 Regulation achieved valve will move to position Closed Z Regulating down valve will move to position B 29 CHAPTER 5 AUTOMATED CONTROL An automated script is a series of steps for controlling one or more automated devices Each
25. LabSmith TOOLS FOR SCIENCE LabSmith uProcess System User Guide For Operation and Control of the following LabSmith uProcess devices using uProcess Software SPS01 Programmable Syringe Pumps AV201 Automated Valves AV202 Automated Valves uPS Pressure Sensors U W mith 4AM01 Analog Sensor Manifold U 7 9 3 o A a 7 e z a g 0 2 Cina in a a AA m e e e a ere mm m m a ma a a sran we CC ma lm ee E ef LabSmith 4VMO01 Valve Manifold C aa Ce ee E peee CE e ma a aa m a Sees per e e e L ZE a a Programmable Syringe Pump eC fC L T ef eC eres 2 K eH e a E ERE a m a a a eee HS Ses G e B EE G 6 a N a BE 6 6 e a 6 a m a a a d siiin WE E TOT a a m a 0 ROSES O e MC CC iaaa lt CCG HHT J E me 6 6 6 6 666 e 66 ee am a a a S Z mec E amp 6 6 6 6666666 ee 64 S amp eie e e 6 6 A R e e o o ooa O14 e eS oa mE Ll d ee sL axe 6111 Southfront Rd Suite E Livermore CA 94551 925 292 5161 ph 925 454 9487 fax www labsmith com This manual 2015 LabSmith Inc uProcess software 2015 LabSmith Inc No part of this document may be reproduced or distributed without the consent of LabSmith LabSmith CapTite uProcess and uDevice are trademarks of LabSmith Inc CHAPTER 1 INTRODUCTION Equipment List Cautions and Warnings Getting Help About This Manual CHAPTER 2 G
26. Manual the LabSmith web site and LabSmith tech support TE Run Pause Quit Control the progress of an automated process O Stop all devicesStop all activity of all uProcess devices 63 4 l H Re scan devicesCheck for all connected uProcess devices IG e Online Establish communication between the EIB and computer Tip You can also use keyboard shortcuts shown in parentheses in the list above to perform the commands To toggle toolbar buttons on or off click the down arrow on the right side of the toolbar then choose Add Remove Buttons gt Standard Choose Reset Toolbar to show all buttons Click and drag the four dots at the left side of the toolbar to tear it from the main window as a floating palette Click and drag the floating palette back into the main window to re dock it Choose View gt Toolbars and Docking Windows gt Standard to show or hide the toolbar Tooltips Each toolbar button and many other buttons in uProcess includes tooltip text that appears when you position your cursor over the button Tooltips explain a tool s function Choose View gt Verbose Tooltips to view more extensive descriptions Figure 29 O Stop All uDevices and Automation Stop all uDevices and stop any running sequence Figure 29 Tooltips Provide Helpful Tool Descriptions Customizing the Dashboard Show Hide Dashboard Elements From the View menu choose Toolbars and Docking Windows From this menu option you ca
27. Set motion via Ch 0 0 0 073 2800 I g g Target volume Flow rate ul 29 452 ul ul min U m gt g 102 ul Set motion via Ch 0 0 0 073 2800 o s Output q x Sequence idle 0 00 00 000 Ready CAP NUM SCRL f Figure 12 Example of Control Overview Window uDevice Controls Individual uDevices can be controlled via the Device Control Overview Window shown in Figure 12 or via its individual Device Control Panel The controls for each type of uDevice are described below Analog Manifold Control The analog manifold controls are used to monitor and control the UPS pressure sensors Typical Control Overview Window and Analog Manifold Control Panel views are shown in Figure 13 and Figure 14 respectively LabSmith 4AM 0041 fj 6 790 kPa W Reg amp 073X YT 7 818 kPa V Reg WF 0665 H 14 094 kPa Reg E 072X D R 200 kPa V Reg Figure 13 Typical Control Overview Window view of analog manifold controls 20 4AM name LabSmith 4AM Four Analog Module Properties Address 67 Configuration f sensor name Online Monitor 0041 22 i 0 kPa 800 kPa eee 25 45 C N 073X a pressure reading y 537 p 0 kPa regulation pressure soo kra rete annan uF K U s U i 24 43 C R indicators 066S 14 085 pa ats 800 kPa Regulate wr F 24 69 C BL 072X a H 0 kPa 800 kPa 25 21 C i L slider bar scale ambient temper
28. Syringes H Dye SetFlowRate 200 ul min 11 46 48 144 Parsing script E Dye MoveTo 100 ul 11 46 48 154 Error on line 35 Device not found water SetFlowRate 100 ul min 1 H Water SetFlowRate 200 ul min 11 46 48 154 Parsing failed with 1 error in 80 lines i 11 46 48 154 All objects declared in the script are connected 11 46 48 154 The script cannot be run until the script errors are resolved TDD script Parsing Keady Figure 25 Sample script showing errors in line Missing Devices All of the devices referenced in a process must be available for the process to function If you open or create a script which calls out a device that is not present error messages will appear in the uDevices Windows the Script Parsing tab of the Output Window and the Main Window Figure 26 41 File Edit View Actions Help lil Interfaces 75 LabSmith E1B01 on COMS 6 LabSmith 4AM Addr 02 a Water Addr 55 LE LabSmith 4VM Addr 63 ee Missing devices 70 014 kPa Be Sequencing erif a al ith AVM 4VMO01 Set motion via Ch 0 0 No valve 3 Open A Close Open B Set via Ch Dye missing but used in script Target volume E 063 RegBetween 305 709 325 709 k D ul min K5 Dye MoveTo 44 000 ul f g lt WaitAllDone gt 80 ul Output Figure 26 Error Message Referencing a missing device For this example the script references Dye b
29. activated you will also need to press the Apply All Settings button to start pause or stop the recording Press to pause the recording press again to unpause and again to stop the recording Depending on the Video File Saving settings you may be prompted for a file name after each recording unless Auto name files is selected 8 To monitor a probe a Note your probes must first be created in the uScope software before they can be imported and monitored in uProcess See the uScope user s manual for instructions on creating probes Once your probe s have been created click on the SVM340 icon in the device tree to bring up the SVM340 device window File Edit View Actions Help Configuration Z Online Video a mm um Specify name Still image Specify name SVM340 4 WIE Interf SVM340 Properties HZ LabSmith 18200 on COM48 Reading E Reg Motion ER 2 813 y J g 25 879 Focus 421 Illumination K A 50 000 S A 50 000 Fica o A 50 000 D epi 50 000 __ Switch to preset oe c Click on Probes to open the probes window Select Probe Select datum from real time image probe open in uScope none D d Click on the dropdown bar to select the probe you would like to monitor in uProcess 59 SVM340 Commands Available in the uProcess Sequencing Script Note This section assumes the user is familiar with th
30. aitAllDone gt Fill_ Syringe Reagent Syringe Reagent Syringe Reagent Manifold lt WaitAllDone gt Open_Process Reagent Syringe Reagent Manifold lt Pause gt 1000 ms Deliver_Reagent Reagent Manifold Reagent Syringe Reagent Syringe lt WaitAllDone gt lt Loop gt Open_Reservoir 15 57 35 832 Parsing script SPSO1 20 ul 4VM01 SetValves 0001 Stop SetFlowRate 50 000 ul min MoveTo 10 000 ul Stop Stop SetValves 0 0 0 3 Stop SetFlowRate 10 000 ulmin MoveTo 0 000 ul 10 15 57 35 842 Parsing succeeded with no errors in 27 lines 15 57 35 842 All objects declared in the script are connected 15 57 35 842 The scriptis ready to run vs lt gt Script Parsing Ready Figure 33 The Complete Example Script Press the Run button to initiate the automated script When a script is running the current step will highlighted in the Main Window to allow tracking of the script progress The status of the devices and the step progression can be monitored real time As shown in the figure below the Device Control Overview Window shows the real time positions of the syringes and valves the Sequence Status tab of the Output Window shows which step is currently running and the number of iterations which have been completed The bottom left corner of the window shows how long the script has been running 47 54 uProcess _ EHi gt LabSmith 18200 on COM44 ITN Reagent M
31. ame defined name of syringe must be defined in script header vol desired syringe volume units volume units acceptable units include ul default ml nl Example Reagent MoveTo 20 5 ul Pause lt Pause gt duration units Pauses script for specified duration Duration length of pause units pause duration units acceptable units include ms default S US Example lt Pause gt 1 s SetFlowRate SyringeName SetFlowRate rate units 36 Sets syringe flow rate SyringeName defined name of syringe must be defined in script header rate syringe flow rate positive number units flow rate units acceptable units include ul min default ul s ni min nl s Example Reagent SetFlowRate 50 ul s SetValves 4VMO1Name SetValves x x x x Stop Quit Sets valve positions 4VM01Name defined name of 4VM0O1 must be defined in script header XXXX position of valves 1 2 3 and 4 respectively where x 0 no change x 1 open valve to A x 2 close valve x 3 open valve to B Example 4VMOl1 A pectVaives 1 0 3 2 DeviceName Stop Stops device DeviceName_ defined name of 4VMO01 or syringe must be defined in script header Example Reagent Stop Note The Stop command is added via the Process Wizard using the x button Stop this uDevice in the Device Control Overview window lt Quit gt Quits the script steps following this function will not be run This function will not stop a device that is
32. anifold Addr 11 VIP Reagent Syringe Addr 35 li Instruments LabSmith EIB200 Reagent Manifold chad oo No valve 1 Set via Ch Z Reagent Syringe Q Target volume a Sequencing Script E Reagent Syringe SPSO1 20 ul f Reagent Manifold 4VM01 B Open_Reservoir B Reagent Manifold SetValves 0 0 0 1 E Reagent Syringe Stop H lt WaitAllDone gt Fill_Syringe E Reagent Syringe SetFlowRate 50 000 ul n E Reagent Syringe MoveTo 10 000 ul E Reagent Manifold Stop lt WaitAllDone gt B Open Process E Reagent Syringe Stop e Reagent Manifold SetValves 0003 E lt Pause gt 1000 ms Deliver_Reagent Reagent Manifold Stop D nn wnt C winna KTL DL ITN NAN olla l gt ELDI script Parsing Sequence Status Communications g 4 982 ul ul min 10 000 ul min m K g Set motion via Ch 0 0 15 51 44 538 Parsing script 15 51 44 538 Parsing succeeded with no errors in 27 lines 15 51 44 538 All objects declared in the script are connected 15 51 44 538 The scriptis ready to run Figure 34 Sequence Status Tab Syringe Pump Volume vs Delivery Time In the above example the syringe is set to deliver 10 ul of reagent at 10 ul min In order not to overshoot the delivery of the reagent the syringe will slow down just before reaching the target volume therefore the delivery time may be more than 1 minute This is
33. ature Figure 14 Typical Analog Manifold Control Panel view The following commands and information are available for each connected uPS pressure sensor e 4AM01 Name Set name for the 4AM01 Sensor Name Set name for the pressure sensor see 4AM and Sensor Configuration Settings for instructions on changing sensor name Pressure Reading Current sensor pressure reading Regulation Pressure The regulation pressure can be set by moving the slider bar or by typing the desired pressure in the pressure reading box Temperature This is the pressure sensor ambient temperature not the fluid temperature Scale Indicates pressure scale for slider bar see 4AM and Sensor Configuration Settings for instructions on changing scale temperature and scale are not displayed in the Control Overview Window Pressure Regulation Regulation Status Indicators show the current regulation status regulating up regulating down regulation satisfied unregulated See Channel Regulation below for more information Clicking on Reg or Regulate brings up the Regulation Settings Window as shown in Figure 15 From this window you can set the regulation target reading and choose a hardware channel to output the regulation status 21 Regulation Settings Target reading H W Check too low W Check too high Tolerance 10 kPa Hysteresis Fal Indicate regulation on hardware channel A c Op Note
34. ch switches valves to fill position 4VM SetValves 3 3 0 U AVM WaitDone celal fills syringes with liquid from reservoirs Water 80 SetFlowRate 150 ul min Water 80 MoveTo 100 ul Dye 80 SetFlowRate 150 ul min Dye 80 MoveTo 100 ul Water 80 WaitDone Dye 80 WaitDone oWitch Dispense 7 Swatches valves to dispense postion AVM SetValves 11 0 U AVM WaitDone Regulate gt dispense the syringes to pressurize the reservoirs when the pressure reaches max set value syringes will stop pumping amp wait until the pressure has dropped below min value Dye 80 SetFlowRate 100 ul min UPS Ls HysteresisReg 45 46 kPa to ChA Dye 80 MoveWith ChA Water 80 SetFlowRate 100 ul min UPS 2 HYSCSresrSReG oy 57 5 KPa TO CHB Water 80 MoveWith ChB CheckRegulation loop that checks if either syringe is empty at gt 0 and switches to refill if so Water 80 i1fdone lt Goto gt switch Fill Dye 30 1fdone lt GOtO gt SER FILI Constant pressure pinched flow example script The above script will run continuously until it is manually stopped 54 CHAPTER 6 SVM340 AUTOMATION AND CONTROL uProcess software versions 1 28 and higher allow control of the SVM340 within the uProcess software The instructions below describe how to use uProcess to connect to and control the SVM340 This guide assumes you have basic operational knowledge of the SVM340 operation and uScope software Please refer to the full uScope user s ma
35. d volume flow rates To select the correct glass size Click on the device in the uDevices Window Select Configuration Choose the syringe pump glass size from the Size drop down list 10 Follow instructions for calibrating below CAUTION Ensure you are using the correct size plunger for your syringe glass Calibrating the Syringe Pump Each SPS01 syringe pump is calibrated before shipment However each glass plunger assembly may have slight length variations up to 1 and there can also be variations in the placement of the glass in the syringe body Each time the syringe glass is removed or moved the syringe pump should be recalibrated Calibrating the syringe pump allows uProcess to find the plunger stopping point thereby minimizing the dead volume within the syringe and allowing correct calculation of the syringe volume Note Calibrating the syringe will run the syringe to its maximum extents and will update its calibration values The procedure may take up to 1 minute To calibrate the syringe Select the device in the uDevices Window Select Configuration to open the Syringe Pump Configuration dialog box Figure 32 68 Configure LabSmith P501 Device Address L 7 Custom Size 230 mm diameter Calibration Full ut 1070 Calibrate Full In 65415 Firmware Hardware wersion 01 02 00 05 Firmware version 01 15 Update Firmware Firmware up to date Figure 32 Syringe Pump Configuration Dialog Box
36. e Instruments tree to show only the SVM340 controls File Edit View Actions Help uDevices SVM340 4 i m V Interfaces SVM340 Properties _ TSR LabSmith EIB200 on COM48 Reading E Reg Configuration Online 9 4VMO1 Addr 04 IS SPS01_80 Addr 26 Motion Video 9155 Instruments x 2 813 mm MIO SVM340 4 0 S 25 879 mm Focus Ki 421 um Specify name Illumination aie A 50 000 Still image B 50 000 Bad L Elicong 50 000 C s Specify name 50 000 D epi Switch to preset 2 To alter the X Y or focus setting a If using the Interfaces window first place a check mark beside the setting you want to change not required in the SVM solo window D Move the slider or type in the desired position Press the Apply All Settings Now button Or press the Update all uDevices Continuously button to continuously update the settings 3 To alter the Illumination setting a Inthe Interfaces window click on Change Illumination and slide the channel bars to the desired value and click OK b if the Continuously Update button is not depressed you will need to press the Apply All Settings button for the settings to be changed 4 Preset values must be set using the front panel for presets A D or from the uScope software A J Once they are set the can be recalled from the SVM340 portal in the uProcess softwa
37. e LabSmith website www labsmith com We recommend visiting the website occasionally to check for software updates CHAPTER 3 uPROCESS SOFTWARE Software Interface The uProcess Dashboard consists of a menu bar toolbar and configurable windows Figure 3 Unique menu and toolbar commands will be described in the body of the text below A complete listing of all menu and toolbar commands can be found in Appendix A Menus Toolbars Window Controls P Untitled uProcess File Edt View Actions Help JIGad uDewices 3 Interfaces uDevices Window Main Window Sequencer Window Tab Y A IN uDevices fe Sequencer Output Output Window N gt N SN Parsing Sequence Status A Communications Sequence idie Ready Figure 3 uProcess Dashboard The uProcess Dashboard consists of 5 windows described below You can customize the placement of the windows on the dashboard and a number of other features about the interface See Customizing the Dashboard for more information uDevices Window The uDevices Window is used to view add and configure individual devices and EIBs Figure 4 It shares the left side of the Dashboard with the Sequencer Window Click the tabs at the bottom of this area to switch between the two windows 10 Br lil Interfaces 7 5 LabSmith EIBO1 on COM6 LabSmith 4AM Addr 02 LabSmith SPSO1 Addr 50 8 LabSmith 4VM Addr 63 i Instruments LS pee i Eames Figure 4 uDev
38. e a script using the Process Wizard or you can create edit the text directly To view and edit the text of a script click on the Sequencer window then select Sequencing Script The text will appear in the Main Window Here is a sample script 39 Syringe DPCOL 20 ul Manifold AVMO1 Open Reservoir Manifold SetValves 1 0 0 U lt WaitAllDone gt Fill Syringe Syringe SetFlowRate 50 ul min Syringe MoveTo 10 ul lt WaitAllDone gt Open Process Manifold SetValves 3 0 0 0 lt Pause gt 1000 ms Deliver Reagent Syringe SetFlowRate 10 ul min Syringe MoveTo 0 ul lt WaitAllDone gt The first lines define the devices used in the script Note that the devices are defined by name rather than address so the names of all devices must be unique Below these declarations each step is listed in order For clarity all state changes within each step are indented below the step name When learning to edit the text it may prove helpful to create a script using the Wizard then to edit the generated text as the basis for your new script Most standard text editing functions are available including Cut Copy and Paste Script Errors uProcess will review the script as you create or edit it and will indicate errors as follows lf the script has an error the Run button will be grayed out and the script cannot be run The Script Parsing tab of the Output window will display the message The script cannot be run until the script e
39. e syringe to increase the pressure Example P0327 RegDownTo 50 kPa to ChA SEeoUL MoveWith ChA Note Use the SetFlowRate command to set the syringe pump flow rate for each syringe else the last flow rate set for the syringe will be used RegOff SensorName RegOff Turns off the regulation for a pressure sensor Example 032 RegOff WaitAllDone lt WaitAllDone gt Pauses script until all processes are complete Example lt WaitAllDone gt 38 General Formatting Rules Devices Individual devices are defined in the script as follows Syringes DeviceName SPS01 size ul Where size is the syringe size 4 8 20 40 or 80 Valve Manifolds DeviceName 4VMO1 DeviceName must match name defined in the uDevice configuration see Device Naming and Numbering Note When referring to a device in the script the device name cannot start in the first column of arow Insert a space or tab before the name Comments Comments can be added to the script using a semicolon Anything after the semicolon is treated as a comment lt Pause gt 1000 ms to allow time to monitor reaction lt Beep gt this command will not be executed Step Name Step names may have a maximum of 16 characters and may not include blanks or lt gt or The Step Name must start in the first column of a row do not add a space or tab before a step name Editing Automated Script Text As mentioned earlier you can creat
40. e total current to 800 mA to account for device and power supply variations Device Idle Active Current Consumption Current Consumption mA mA EIB200 uProcess Interface 4VMO1 Valve Manifold AV201 AV202 or AV303 Automated Valve 4AM01 Analog Manifold uPS Pressure Sensor SPS01 Syringe Pump Notes 1 Current values are for 12 V power supply 2 Uncertainty 3 mA 3 Valid for use with EIB200 only current consumption will be higher with EIB100 4 EIB200 supplied with 12 V 1000 mA power supply 71 APPENDIX D CAPILLARY AND TUBING INSTALLATION GUIDE LabSmith s CapTite one piece fittings enable connection of components valves syringe pumps interconnects bonded port connectors Luer Locks and cartridges to 360um capillary 1 32 tubing or 1 16 tubing To install the capillary or tubing Cut the capillary or tubing to length Please refer to the capillary or tubing packaging for cutting instructions Insert the capillary into the nut end of the one piece fitting until the tubing extends through the tapered ferrule end approximately 2 mm Insert the one piece fitting into the component and turn the fitting to finger tighten CAUTION Do not over tighten the fitting Fittings should only be finger tight to avoid damage the 1 8 hex wrench can be used to tighten or loosen difficult to reach fittings Gently pull on the capillary tubing to ensure that it is held securely by the fitting If
41. e uProcess automated script functions If not please review Chapter 5 before continuing The uProcess script wizard can be used to automatically configure the script file and add many of the commands listed below The SVM340 commands are added by manually adjusting the settings in the interface window and then clicking the Save as New Sequence Step button Aa The following header must be included in the scrip file when using SVM340 commands SVM340 SVM340 where is the SVM340 com port number All commands to the SVM340 will have format SVM340 COMMAND All available commands are listed below Video and Image Saving NOTE uProcess has the option to specify a file name in the script when saving an image snap shot time averaged image or video If the file name is NOT specified uProcess will use the naming configuration that has been set up in uScope File gt Snap Shot File Saving or File gt Video File Saving If the file name IS specified a file name or address with spaces in it MUST use quote around the name see Recordimage below for example Recordimage lt optional filename gt takes a snapshot image Averagelmage On lt optional filename gt _ starts a time averaged snapshot image Averagelmage Off stops a time averaged image RecordVideo On lt optional filename gt Starts video recording RecordVideo Off Stops video recording PauseVideo On pauses recording PauseVideo Off resu
42. ee EIB200 e eee eee e amp EF e 6 6 6 a a o L 2 oe T amp uPB 05 Breadboard us S ee e eee e 6 o o s reserved gt oO w Figure 1 uProcess System Components Equipment List Required Equipment PC compatible computer running Windows 7 or newer uProcess Interface EIB200 uProcess software installation disk included with EIB purchase 12V power supply for EIB included with EIB200 purchase micro USB cable included with EIB200 purchase Additional required and optional equipment for syringe pumps SPS01 Programmable Syringe Pump SPS01 syringe glass plunger set included with SPSO1 purchase CapTite ferrule C360 100 or T116 100 for connecting to capillary or tubing syringe glass plunger installation tools plunger wrench and 3 32 hex wrench installation screws qty 2 x M3 optional Additional required and optional equipment for automated valves AV201 AV202 or AV303 automated valve 4VM01 valve manifold one manifold per up to four valves flat ribbon connector one per valve 4 cables included with 4VM01 purchase Three 3 CapTite ferrules C360 100 1132 100 or 1116 100 for connecting to capillary or tubing breadboard mounting screws 2 28x1 4 two per valve optional Additional equipment required for pressure sensors uPS pressure sensor 4AM01 analog manifold one manifold per up to four sensors flat ribbon connector one per sensor 4 cables included w
43. es have finished Select Loop to Step and choose Open_ Reservoir from the drop down menu Set the loop count to 10 and the process will repeat for 10 iterations Creating a new sequence step New step Deliver_Reagent After changing the uDevice settings Pause 4000 ms Wait until all devices are finished Do not wait Continue End if this is the last step Loop to step Open_Reservoir L 10 times 0 Figure 32 Deliver_Reagent Step Settings Click OK Again select the Sequencer Window tab and click on a step in the script to view the complete script 46 PI uProcess File Edit View Actions Help v S wis Interfaces E ae LabSmith EIB200 on COM44 RN Reagent Manifold Addr 11 md Reagent Syringe Addr 35 wu Instruments m B ka Sequencing Script E Reagent Syringe SPSO1 20 ul fff Reagent Manifold 4VM01 fi Open_Reservoir H Reagent Manifold SetValves 0001 fff Reagent Syringe Stop H lt WaitAllDone gt offi Fill_Syringe H Reagent Syringe SetFlowRate 50 000 ul n E Reagent Syringe MoveTo 10 000 ul ff Reagent Manifold Stop B lt WaitAllDone gt ffi Open Process z Reagent Syringe Stop ff Reagent Manifold SetValves 0 0 0 3 E H lt Pause gt 1000 ms offi Deliver_Reagent B Reagent Manifold Stop E E E RE AE a PT EEE T T ET I Reagent Syringe Reagent Manifold Open_Reservoir Reagent Manifold Reagent Syringe lt W
44. h SPS01 Addr 16 Target volume Flow rate E Instruments A ul 16 395 E P G U E A 54 ul Set motion via Ch 0 0 Target volume Flow rate ul 55 578 ul ul min oo E Set motion via Ch 0 0 LabSmith 4VM Valve 1 No valve 2 Valve 3 No valve 4 v Open A OpenA Close Close F open 8 F lopen B Set via Ch Set via Ch LabSmith EIB01 i ch a e chp 7 cho d chp e LabSmith SPS01 Target volume Flow rate ul 53 457 ul ul min ee P Set motion via Ch 0 0 Sequence idle Figure 7 uProcess Dashboard The uDevices Window contains a tree structure listing each EIB and the devices installed under each In Figure 7 two EIBs are connected Two SPS01 syringe pumps and a 4VM are connected to the EIB on COM6 A SPS01 syringe pump is connected to the EIB on COMS Note Breadboards will not appear as device in the uDevices tab since there are no functions to control on the breadboard itself With the uDevice window selected the Main Window will show one of two views the Control Overview Window or the Individual Device Control Panel The Control Overview Window allows limited control of all devices It is also the view that will be used to program sequences using the programming wizard see Create a Script Using the Wizard By clicking on one of the individual device names in the uDevices Window the Individual Device Control Panel opens Figure 8 shows the control panel f
45. he SVM Is operational you will return to the uProcess software 9 If SVM340 0 is listed under instruments right click on the name and select Remove this Instrument 10 Right click on Instruments in the uDevices tree and select New Instrument gt SVM340 to open a SVM portal SVM340 Options These settings apply if uScope is not already open uScope V 1 45 or greater is required Optionally specify the com port COM Optionally specify a probe usc file to e 11 Leave the COM port blank 12 Click OK 13 If the uDevices window shows SVM340 and the number is non zero you are connected to the SVM go to the next section Using the SVM in uProcess 14 If the SVM340 Icon has a red circle slash through it and the number is zero as shown on the right you are not connected to the SVM In this case right click on the SVM icon and select Remove this Instrument close the uProcess and uScope software and start again on step 1 Contact LabSmith to if you still cannot connect uDevices oe WIE Interfaces NE Interfaces Lom Eli Instruments Real Instruments 3 Saare MIO SVM340 8 56 Using the SVM340 in uProcess 1 The SVM controls are viewed one of two ways Each view has slightly different control options a Click on the Interfaces icon in the uDevices tree to show all devices connected via EIB or external instruments b Click on the SVM340 icon in th
46. ices Window Sequencer Window The Sequencer Window is used to view a sequence program Figure 5 The uDevices Window is used to view add and configure individual devices and EIBs It shares the left side of the Dashboard with the Sequencer Window Click the tabs at the bottom of this area to switch between the two windows amp Sequencing Script E 0067 uPSO1 800 kPa E 0110 uPSO1 800 kPa j 0061 uPS01 800 kPa 2 E LabSmith 4AM 4AM01 H Water SPSO1 40 ul B Dye SPSO1 80 ul L H LabSmith 4VM 4VM01 L Step1_Open_Valves_to_Reservoir 0067 RegOff E 0110 RegOff 3 LabSmith AVM SetValves 1010 ff lt WaitAllDone gt E Step2_Fill_Syringes E Dye SetFlowRate 200 ul min fH Dye MoveTo 100 ul E Water SetFlowRate 200 ul min E Water MoveTo 50 ul H lt WaitAllDone gt E Step3_Open_Valves_to_Chip Hl Figure 5 Sequencer Window Output Window The Output Window is used to monitor device communications or to debug and monitor a sequence program Figure 6 By default this window appears at the bottom of the dashboard 1 11 49 26 667 ScriptRunning Stepl_Open_Valves_to_Reservorr iteration 1 r 11 49 26 727 Script Running Step2_Fill_Syringes iteration 1 11 49 34 508 Script Stopped Script stopped with 0 errors after executing 18 lines in 2 steps 11 54 35 616 Script Message Script started 11 54 35 626 Script Running Stepl_Open_Valves_to_Reservoir 11 54 35 676 ipt Running
47. ing its set possition Configure LabSmith 4 Name Tea hs EVA Device Address Actuation profile settings Initial knob 100 8 100 Stall holdoff 3 3 Start Current 120 11201 Duration 150 150 Cruise Current 192 192 Duration 140 140 Slew 135 135 Duration 10 10 End Current 151 151 Duration 250 250 Backlash ms 120 120 Check pos 240 default Firmware Hardware version 02 00 00 06 Firmware version 00 0d Firmware up to date Figure 31 Configure Device Dialog Box for a 4VM01 Valve Manifold 66 Changing the Syringe Pump Glass Assembly The SPS01 syringe glass and plunger can be removed from the pump body to be cleaned or exchanged with a different syringe size To remove the syringe glass and plunger 1 Supply power to the syringe pump 2 Inthe uProcess uDevices Window select the syringe 3 Enter a Set Point of 0 to extend the syringe plunger to the 0 volume position Reagent Syringe Q Pump Properties Address 2 Syringe Size Standard 20 ul Af online Monitor ae 2 Click Apply to extend plunger lt N s c ul min 0 186 1130 Position Monitoring amp Control ul Set Point mm m 0 000 ul 21 ul Ddu 5s ul Actual 1 Enter 0 Setpoint 4 Click Offline to take the syringe offline 5 Unplug the syringe from the breadboard 6 Using the supplied hex wrench loosen the screw located on the side of the syringe 7 Gently pull the syringe glass straight
48. ith 4AM0O1 purchase CapTite component C360 1132 or T116 with female port for installing sensor Optional Equipment 20 pin communication cable for direct connection of EIB to uDevice included with EIB200 purchase UPB or iBB breadboard Cautions and Warnings When working with uProcess devices and accompanying electronics follow standard procedures to protect your devices from excessive static electricity power fluctuations etc Read and understand all documentation for your individual uProcess devices and components before connecting the equipment CAUTION Extra care must be taken when using the electronic components with fluids Breadboards are designed with the electronics mounted on one side use precautions when introducing liquids to this side of the breadboard Getting Help This guide is your main source for information on operating uProcess software Choose Help gt About uProcess then click the Open User Manual link to browse this manual electronically in Adobe Acrobat PDF format The LabSmith web site www labsmith com also includes helpful applications notes and other technical information to help you use uProcess software and devices If you are unable to find the information you need please contact our technical support team at 925 292 5161 or email us at suppori labsmith com Be prepared to give the software version To determine the software version number select Help gt About uProcess About
49. l Overview Window tA uProcess Vis Interfaces 9 Gi LabSmith 18200 on COM44 LabSmith EIB200 WIN 4VM01 Addr 11 26 LabSmith SPSO1 Addr 35 y a bd pada Gi W I Instruments 4VM01 G No valve 1 No valve 2 No valve 3 Valve 4 Open A Close Open 8 Set via Ch Target volume Flow rate amp Sequencing Script ul 11 746 ul ul min as k Set motion via Ch 0 0 560 14 47 57 007 Parsing script 14 47 57 007 Parsing succeeded with no errors in 0 lines 14 47 57 007 All objects declared in the script are connected 14 47 57 007 The scriptis ready to run ala KR Script Parsing Sequence Status Communications Sequence idle Ready Figure 278 Installed Devices Shown in Device Control Overview Window 43 Set the position for Valve 1 on Reagent Manifold to Open A Depress the Do Not Change uDevice Settings button next to the Reagent Syringe Click the Save As a New Sequence Step button Name the new step Open_Reservoir Select Wait until all devices are finished and Continue Click OK to add this step to the script and go on to the next step Set the Target Volume for the syringe named Reagent Syringe to 10 ul and the Flow Rate to 50 ul min PI uProcess File Edit View Actions Help _ g vie Interfaces TK LabSmith 18200 on COM44 9 Reagent Manifold Addr 11 I Reagent
50. mes recording Examples 60 SVM340 3 RecordVideo On SVM340 3 RecordVideo On C video1 jpg SVM340 3 RecordVideo On C saved videos video1 jpg Focus Stage Position and Illumination Adjustment MoveToxX x units moves to position x units from center MoveToyY z units moves to position y units from center Focus To z units moves to position z units from center MoveTo x units y units z units moves to position x y z MoveByX dx lt units gt moves dx units from current position MoveByY dy lt units gt moves dy units from current position FocusByZ dz lt units gt moves dz units from current position Acceptable units mm um in cm m Examples 3 MoveToxX 5 mm 3 MoveByY 0 2 mm SVM340 3 FocusTo 500 um 3 MoveTo 5 mm 10 mm 500 um SetLEDs C1 C2 C3 C4 sets illuminator channels intensity from off 0 to full 1 0 Example SVM340 3 SetLEDs 0 5 0 5 0 0 1 0 SwitchToPreset 1 to 10 moves to preset position illumination setting 1 A 2 B etc Example SVM340 3 SwitchToPreset 2 Stop Stop all motion Example SVM340 3 Stop Regulation using Probe Data SelectReading ProbeName ProbeDetails reads probe data for regulation Use with one of the following regulation commands RegUpTo value RegDowntTo value 61 62 RegBetween min_value max_value Examples SVM340 4 SVM340 4 SVM340 4 SVM340 4 SelectReading Polygoni MeanGra
51. most pronounced when delivering at high flow rates For some processes the infuse time may be more critical than the total delivery volume For this case you can use the lt Pause gt and lt Stop gt functions together to set the infuse time The script from the above example is shown below In this script the syringe is called Syringe and the 4VM01 manifold is called Manifold 48 Syringe SPOUT lt 207 al Manifold AVMO1 Open Reservoir Manifold SetValves 1 0 0 U lt WaitAllDone gt FELI Syringe Sy rere SetFlowRate 50 ul min Syringe MoveTo 10 ul lt WaitAllDone gt Open Process Manifold SetValves 3 0 0 U lt Pause gt 1000 ms Deliver Reagent Syringe SetFlowRate 10 ul min Syringe MoveTo 0 ul lt WaitAllDone gt The total volume delivered is 10 ul which at 10 ul min should take approximately 1 minute Ifa delivery time of exactly 1 minute is desired the script could be modified as follows changes are in bold Syringe SPSOL Z0 w1 Manifold AVMO1 Open Reservoir Manifold SetValves 1 0 0 U lt WaitAllDone gt Pile yrange Syringe SetFlowRate 50 ul min Syringe MoveTo 11 ul provide extra volume so the syringe won t stop prematurely lt WaitAllDone gt Open Process Manifold SetValves 3 0 0 U lt Pause gt 1000 ms Deliver Reagent Syringe SetFlowRate 10 ul min Syringe MoveTo 0 ul lt WaitAl1lDone gt this command is commented out lt Pause gt 60 s allow the syringe to run f
52. n choose to show or hide the toolbar uDevices Window Sequencer Window and Output Window Auto Minimize Windows Click the push pin button L at the upper right of the uDevices Window Sequencer Window or the Output Window to minimize the window to the edge of the screen Roll over a minimized window to make it reappear it will automatically minimize when you roll the cursor off of the window The push pin button will be horizontal in this auto minimize mode Click the button again to turn it vertically and disable auto minimizing Detach Dock and Re Position Windows Click and drag the top of the uDevices Window Sequencer Window or the Output Window to detach it from the Dashboard and create a floating window Drag the floating window over the Dashboard to dock it Positioning guides will appear as you do so to help you attach the window at the top bottom left or right of the uProcess Dashboard Figure 30 64 B 0 al OD uDevices x iy Valve Menitok BS egg Four Valve Driver S Interfaces address l 5 amp EIB on coms ane P Reagent Syringe Addr 02 ai 4 Dock at Top faton onine M Monitor P Sample Syringe Addr 16 Vave 1 Yatve 4 No change wo change Oa Oa O closed Odosed a Os os d L3 Indeterminate inate Indeterminate a w Dock at Left Side Dock at Right Side gt 15 57 38 357 Parsin 15 57 38 357 Parsin 15 57 38 357 Al uD
53. nts P 26 47 26 47 C 0110 0 0003 kPa 800 kPa F Regulate Epey arg r E 31 51 31 84 C 0061 0 0001 kPa 800 kPa Regulate E F 25 87 42 80 C No sensor 4 fB Sequencer Sequence idle Figure 10 Control Panel for Analog Manifold uPS pressure sensors installed in positions 1 2 and 3 15 Online and Offline The communication status of each device is shown in the uDevices Window To monitor or control a device the device its EIB and the interface must all be online As shown in Figure 11 the icon to the left of each device will indicate whether it is Offline not in communication with the computer or Online in communication In this example the EIB on COM11 is offline and the EIB on COM1 is online To change the status of a device or EIB right click the device in the uDevices Window and check uncheck the Online setting To toggle all connected devices to online or offline click the Online toolbar button Note An EIB will go offline if there is a communication error between the COM port and the EIB Two red lights on the EIB also indicate a communication problem This error often indicates a bad cable connection The Communications tab of the Output Window lists the EIB port communication status and history rd Untitled uProcess File Edit View Actions Help ae ed 6 k O uDevices Ax LZ lt S Interfaces ig EIB on COM11 af LabSmith sP501 Addr 27
54. nual for detailed SVM340 instrument and software instructions Software Requirements You must have the following software installed on your computer to use the SVM340 in uProcess uScope version 1 45 or later uProcess version 1 28 or later After downloading the latest uScope software update the SVM firmware by going to SVM gt Update firmware gt Update all Connecting to the SVM340 in uProcess 1 Start the uProcess software If you would like to connect to uProcess equipment via an EIB you may do so at this time 2 Turnon the SVM340 and make sure the RS232 communication cable is connected to the computer either to a RS232 port or to a USB port through a RS232 USB adapter Start the uScope software 4 Ifthe serial port is not found automatically the Select a new serial port window will open If this serial port window does not open the correct com port has been found and you can skip to step 6 Dae Select a new serial port Error opening serial port select new Serial port 5 Select the correct serial port from the dropdown list and click OK refer to the SVM user s manual if the correct serial port cannot be found 6 Once the correct serial port has been found you will select the optics module If the window to open a video file automatically opens click Cancel to get to the uScope main window 7 Select Video gt Video Capture Device to select the installed optics module 55 8 Once t
55. or 60 s Syringe Stop stop the syringe lt hoop gt Fill Syringe 10 In the second example the syringe is instructed to stop after exactly 60 seconds which will be after approximately 10 ul is delivered 49 Pressure Regulation Example Scripts The uPS pressure sensors and SPS01 syringe pumps can be used together with the uProcess software to regulate the pressure in a device or create pressure driven flow In the simple pressurization example below the SPSO1 syringe pump is filled from the reservoir and then it is used to pressurize a device When the pressure reaches 100 kPa the script is complete reservoir SPSO1 Programmable Syringe Pump Simple pressure regulation application uPB O5 breadboard 4VM manifold and 4AM manifold not shown for clarity In order to rename the pressure sensors click on 4AM in the uDevices window under Interfaces In the main window click on Configuration This will open up a new dialogue box Click on Sensor 1 to edit the name of the first sensor This will open another dialogue box In the box under Sensor Name put in the new name In this script the sensor is called uPS Remember the name is case sensitive 50 Simple Pressure Regulation Example Script OP SO I pE S 4AM AVM SWLeech ELLI AVM AVM ELEL SPOOL SPoUL SPOOL Switch Dispense 4VM 4VM Pressurize UPO SPS01 Seo Od SPSO1 oO wL uPSO C00 kPa 4AMO1 AVMO1 E s
56. or a syringe pump Figure 9 shows controls for a 4VM01 valve manifold Figure 9 shows controls for a 4AM01 analog 13 manifold The Individual Device Control Panel provides a wider range of control and setting options for an individual device than is available in the Control Overview Window Note Instructions for using the controls in each of the control panels are given in Chapter 4 File Edit View Actions Help lll Interfaces LabSmith EIBO1 on COM6 f LabSmith SPSO1 Addr 50 1 LabSmith 4VM Addr 63 fl Instruments a Devices i Ea Sequence idle 14 LabSmith SPS01 Pump Properties Address 50 Syringe Size Standard 40 ul Speed Control 0 036 Position Monitoring amp Control Set Point J 54 ul oog Configuration E Set motion via Ch 0 0ul 7 913 ul a rr Figure 8 Control Panel for Syringe Pump Actions Help Interfaces _ LabSmith EIBO1 on COM6 LabSmith 4VM Four Valve Driver Properties Address 63 Configuration Online Monitor Valve 1 Valve 3 No change No change A E Closed Odosed M B B E with with Opp Indeterminate L A A In position _ we uDevices BTS Il Interfaces Four Analog Module Properties 75 LabSmith EIB01 on COM6 ess Configuration on Winia LabSmith SPS01 Addr 50 0067 800 kPa 8 LabSmith AVM Addr 63 0 0000 kPa _ Regulate E Instrume
57. ously actuated is limited to approximately 10 based on the current capacity of the EIB200 power supply See Appendix C for information to calculate the current consumption for a particular setup Multiple EIB200s can be used at one time when a large number of devices or concurrent operations are required To connect an EIB200 to the computer e Use the included mini USB cable to connect the COM port on the EIB200 to the computer s USB port e Connect the included power transformer from the EIB200 to a wall outlet The two indicator lights will flash wnen powered up after which the Power light will stay illuminated red The COM light will illuminate when the EIB is communicating with the computer Adding a Breadboard An EIB200 can be connected to a single syringe pump valve manifold or analog manifold however it is typically used with one of the LabSmith breadboards uPB 5 uPB 8 UPB 12 or IBB for mounting multiple components Figure 2 Between 5 and 12 uDevices syringe pumps valve manifolds analog manifolds can be connected to a single breadboard The EIB200 is connected to the uDevices in one of the following ways 1 Plugged directly into a slot on a uProcess breadboard uPB 05 uPB 08 uPB 12 or IBB Connected to uProcess breadboard via a communication cable Connected directly to a uDevice SPS01 4AM01 or 4VM01 via a communication cable Note the standard communication cable provided with the EIB200 can connect only 1
58. r up the LED on the SPS01 will flash red green and then go out Installing Pressure Sensors To install and configure uPS pressure sensors e Plug the 4AM01 sensor manifold into one of the connectors located along the side of the breadboard Secure the pressure sensor s to the desired component Note the uPS360 sensors can be installed in a C360 or T132 CapTite component for use with 360um OD tubing or 1 32 OD tubing respectively The uPS116 sensors can be installed in a 1 116 component for use with 1 16 OD tubing Use a ribbon cable to connect the pressure sensor to a port on the analog manifold Observe the label on the manifold to ensure the cable is connected at the correct orientation Note the orientation of the connector on the 4AMO01 is the opposite of the orientation of the connector on the 4VMO0O1 Connect the ribbon cable to the uPS sensor with the following orientation Upon power up the LED on the 4AM01 will flash red green and then go out Installing uProcess Software uProcess software runs on any PC compatible computer running Microsoft Windows 7 8 or 10 The software requires approximately 5MB of free disk space To install uProcess Insert the uProcess for Windows CD Double click SETUP EXE and follow the on screen instructions To run the software double click the uProcess icon on your desktop or choose Run gt uProcess gt uProcess The uProcess software can also be downloaded from th
59. re 5 To take a Snapshot Image d 57 58 6 Totake a Time averaged Image a Leave the box next to Name blank The image name will use the protocol specified in uScope in uScope go to File gt Snap Shot File Saving The snapshot will use the setting specified in uScope under View gt Snap shot Settings Press to save a snap shot If the Continuously Update button is not depressed you will also need to press the Apply All Settings button to take the snapshot Depending on the Snap Shot File Saving settings you may be prompted for a file name after each snapshot unless Auto name files is selected When you are done taking snapshots you will need to press the Do not Change uDevices button to exit snapshot mode Follow the instructions above for Snap shot image However the image will be time averaged until the a button is pressed for a second time Again if the Continuously Update button is not activated you will also need to press the Apply All Settings button before both starting and stopping the time averaged image 7 Totake a Video a b Leave the box next to Name blank The video name will use the protocol specified in uScope in uScope go to File gt Video File Saving The video will use the setting specified in uScope under View gt Video capture Settings Press to start recording a video NOTE if the Continuously Update button is not
60. ressure starts above regulation range uProcess will regulate down to Pmin and then let the pressure drift up to Pmax DeviceName defined name of uPS pressure sensor must be defined in script header Pmin minimum pressure in range Pmax maximum pressure in range units pressure units Example HysteresisReg 40 000 60 000 kPa HysteresisReg to Channel DeviceName HysteresisReg Pmin Pmax units to Chx 34 Similar to HysteresisReg except it sends the output value to a specified channel that can be used to control a SPSO1 syringe pump Requires two lines of code DeviceName HysteresisReg Pmin Pmax units to Chx SyringeName MoveWith ChxX or SyringeName MoveOpposite Chx DeviceName defined name of uPS pressure sensor must be defined in script header Pmin minimum pressure in range Pmax maximum pressure in range lfDone units pressure units SyringeName defined name of syringe pump must be defined in script header X channel A B C or D MoveWith ChX pulls the syringe to decrease the pressure pushes the syringe to increase the pressure MoveOpposite ChX pushes the syringe to decrease the pressure pulls the syringe to increase the pressure Example HysteresisReg 40 000 60 000 kPa to ChA SPSL MoveWith ChA DeviceName IfDone If regulation is finished the immediately following step will be executed otherwise the immediately following step will be skipped DeviceName defined name of uPS pressure sensor must be defined in
61. rrors are resolved The Script Parsing tab of the Output window will also list the line number and specific error The step with the error will show a broken icon in the Sequencing Script list In the example shown in Figure 25 line 36 has an error Device water is not found because the device was initialized as Water 40 Actions Help 800 kPa dye reservoir pressure sensor 800 kPa water reservoir pressure sensor 800 kPa water line pressure sensor between syringe and valve _ 7 5 LabSmith EIBO1 on COM6 40 ul LabSmith 4AM Addr 02 Water Addr 50 i I Dye Addr 55 Stepl_Open_Valves_to_Reservoir 0067 RegOff lf LabSmith 4VM Addr 63 0110 RegOff LabSmith 4VM Set Yalves 101 0 Instruments lt WaitAllDone gt LabSmith 4 M 4V M01 Step2_Fill Syringes Dye SetFlowRate 200 ul min Dye MoveTo 100 ul Water SetFlowRate 200 ulmin Water MoveTo 50 ul lt WaitAllDone gt _ if Step3_Open_Valves_to_Chip uPSO1 800 kPa LabSmith AVM SetValves 3 0 3 0 uPSO1 800 kPa lt WaitAllDone gt uPS01 800 kPa Step4_Pressurize_Reservoirs 0067 RegUpTo 32 kPa to ChC E LabSmith 4AM 4AM01 Dye SetFlowRate 100 ulmin E Water SPS01 40 ul Dye MoveWith ChC 0110 RegUpTo 27 kPa to ChD SetFlowRate 100 ul min Water MovelWith ChD i lt Pause gt 20 s 0067 RegOff 0110 RegOff Step6_ Check Water_Pressure lt Pause gt 1 s 0067 RegUpTo 5 kPa E Step2_Fill_
62. s the step starts If you select Wait until all devices are finished uProcess will wait until all devices have completed their functions before continuing on with the process select Do Not Wait to immediately continue on to the next step select how the script should continue after this step Select Continue to continue to the next step in the process If a new step is not added the process will end Select Loop to step to jump to another process step Select the target step from the drop down list then enter the number of times the step should loop if 0 is entered loop will be infinate Click OK The step will appear in the Sequencer Window and its text will appear in the Main Window Save a Script File Click the Save toolbar button or choose File gt Save to save all changes to the current script file If the file has not yet been saved you will be prompted to provide a name and file location for it Choose File gt Save As to store the current script under a new file name Run Pause and Quit a Script When a script is open and error free the Run toolbar button will be available Click Run to initiate the process All steps will be performed in order 32 While a script is running you can click the Pause JJ toolbar button to pause the automated process Click Pause again to continue the script from that point Click the Quit Process 4 toolbar button to quit running the automated script Note When you Pause or Qui
63. ss automated devices work together with CapTite microfluidic interconnect products to make it easy to build and rebuild micro and nano fluidic circuits All products are inter compatible and quick to assemble taking the hassle out of fluid routing e SPS01 Programmable Syringe Pumps deliver volumes up to 100 microliters with resolution as low as 2 nanoliters The pump body design allows syringes to be easily removed and replaced providing a wide range of volumes with a single pump e AV201 Automated Selector Valves provide 2 position 3 port switching with zero dead volumes and low swept volumes e AV202 Automated Selector Valves provide 2 position 4 port switching with zero dead volumes and low swept volumes e AV303 Automated Selector Valves provide 2 position 6 port switching with zero dead volumes and low swept volumes e uPS Pressure Sensors provide pressure sensing and regulation The uProcess Software provides a simple interface for configuring and controlling uProcess devices and for creating automated processes for coordinated control This user guide will show you how to connect configure and control uProcess devices how to install and operate uProcess software and how to create automated flow control processes uPS01 Pressure Sensor oto s 3 5 5 BBRES Breadboard Reservoir a ae lt s e eceresnr eee e S eee5aeea4g4ee Me e AV201 Automated Valve S E H ee4ee eeeee50v
64. syringe after use with an appropriate solvent or cleaning agent Following the use of a cleaning agent rinse the syringe with deionized water and finally acetone Wipe the exterior surfaces of the syringe barrel and needle dry with a lint free tissue Make sure that there is no residual cleaning agent in the syringe before using or storing the syringe Do not soak or submerse the entire syringe in any cleaning agent Prolonged contact with cleaning agents may damage bonded parts Sterilizing Syringes may be sterilized with appropriate sterilizing agents such as ethylene oxide Autoclaving Syringes can be autoclaved up to a temperature of 115 C However please be aware that over time autoclaving will shorten the syringes life expectancy Avoid rapid temperature changes which can lead to splitting of the syringe s glass barrel Stainless steel expands faster than glass upon heating so for most terminations autoclaving strains glued surfaces and may eventually lead to adhesive deterioration and leakage The plunger should be removed from the syringe before autoclaving 70 APPENDIX C uDEVICE CURRENT CONSUMPTION The table below shows the approximate current consumption of each uProcess device These values can be used to calculate the total current consumption for a process and determine whether an application can be run with a single EIB200 The power supply for the EIB200 supplies 1000 mA of current however we recommend limiting th
65. t a script devices that were moving will continue until they reach their target states Click the Stop All Devices Q toolbar button to stop all device activity a While an automated script is running the Sequence Status tab of the Output Window will indicate the current status of the script and will report any errors encountered Figure 24 Output 20 42 21 075 Script Message Script started 20 42 21 875 Script Running 20 42 26 640 Script Running Deliver_Reagent Figure 24 Sequence Progress Tab of the Output Window 33 Automated Script Functions Beep lt Beep gt Plays an audible sound the sound associated with the asterisk in System Sounds Example lt Beep gt Note The Beep function cannot be added using the Process Wizard Break lt Break gt Automatically activates the pause 4 toolbar button pausing Sequence until the pause toolbar button is pressed This function is useful for testing a script or if you have an event with unpredictable timing that needs to be controlled manually Example lt Break gt Note The Break function cannot be added using the Process Wizard Goto lt Goto gt SteoName Creates a loop back to named step SteoName defined name of step in script Example lt Goto gt FillStep HysteresisReg DeviceName HysteresisReg Pmin Pmax units Regulates between two pressures If pressure starts below regulation range uProcess will regulate up to Pmax and then let the pressure drift down to Pmin If p
66. the capillary comes free loosen the fitting and repeat steps 2 and 3 If the fitting leaks or the capillary cannot be secured the following troubleshooting items should be checked prior to further tightening the fitting The capillary may be cut too short and so it is not protruding through the end of the fitting when installed The capillary may not have a clean cut An angled or jagged edge will cause the fittings to leak Broken capillary or other debris may be stuck inside the fitting or component Try flushing the port from the opposite side to clear the debris If pressures greater than 10 000 psi are required first finger tighten all fittings and test for leaks at low pressure Then use the 1 8 hex wrench to tighten the fittings approximately 1 4 extra revolution Figure 33 shows capillaries that are properly and improperly installed Red capillary does not protrude through the one piece fitting The fitting will leak and also there is a chance of compressing and damaging the tip of the one piece fitting during installation Yellow capillary may properly seal but the capillary is not fully seated in the interconnect creating dead volume Green capillary is properly installed Figure 33 T Interconnect Showing Properly Installed Capillary Green Improperly Seated Capillary Yellow and Capillary Not Protruding Through Fitting Red Ta
67. tting is used to optimize the gain for device and scale range of interest For the uPS pressure sensors the gain readings should be applied as follows e Off does not sample sensor e xi not applicable for uPS pressure sensors e x20 for full scale pressure readings default for uPS pressure sensors e x200 for applications where the pressures will be up to a max of 10 of full scale Therefore for a uPSO1 800 pressure sensor if the measured pressures are expected to be less than 80 kPa use the x200 gain setting for the most accurate results The default sampling rate is 1 8 kS s This rate has the lowest noise and will be suitable for most applications Zero Offset The zero reading on the pressure sensors will drift slightly typically less than 2 kPa over time especially if there are large swings in the sensor temperature We recommend that you occasionally re zero the sensors to eliminate the zero offset Procedure 1 Power up the sensors and 4AM and wait for about 5 minutes for the 4AM to warm up 2 Click on each sensor to bring up its Sensor Properties window Figure 1 7 3 Click the Zero Reading button to zero the pressure sensor 24 Sensor Properties Sensor type Sensor subtype Sensor name UPS Pressure 100 to 700 kP lt 0131 Default gain Display Units Conversion factor 1 Units text kPa Offset 0 Zero Reading Display limits Minimum Calibration Save Calibration to File Set
68. ulated ai Regulation pending but not set For the example shown below e uPS 0041 is unregulated e uPS073X is regulating up and set to hardware channel B e uPS 066S is pending but not set so the regulation type is unknown the user would press apply settings now to set the programming e uPS 072X is regulating down and is set to hardware channel C LabSmith EIB01 Ch A e Ch B Ch C amp Ch D e LabSmith 4AM 0041 T 7 387 kPa Reg E 073X jj 8 772 kPa V Reg 9 066s jj 13 203 kPa V Reg P 072X 4 146 kPa M Reg 4AM01 and Sensor Configuration Settings Several configuration settings are available to optimize performance of the 4AM01 and pressure sensors Click on the Configuration button of the 4AM01 device window to open the Configure LabSmith 4AM window shown in Figure 16 23 r Configure LabSmith 4AM T Name El e Device Address la v Sensor Properties Sensor 1 Sensor 2 Off Off Off Off x1 xi x1 x1 Gain x20 x20 x20 x20 x200 x200 x200 x200 I Default Default Default Default Use same gain for all sensors preferred for fastest sampling Sampling Rate 1 8 kS s 6 kS s 15 kS s 23 kS s lower faster response Firmware Hardware version 03 00 00 06 Firmware version 00 0e Firmware up to date Update Firmware Serial Number 4AM000000040913 Cotas KTE Figure 16 4AM01 Configuration Settings The gain se
69. ut the syringe is named Water This error can be resolved by renaming the device see Device Naming and Numbering or changing the script To change the script Right click on Dye under the Missing devices tab and select Rename Change the name to Water and click OK Example Script In the example shown in a syringe pump is attached to an automated valve a supply reservoir and a downstream process The syringe will first pull fluid from a reservoir connected to the A port of the valve The valve will then switch to the B position and the syringe will deliver the fluid to the downstream process 42 Reservoir A Syringe Pump Downstream Process Figure 27 Hardware Setup for the Sample Process For this example the following devices are installed on one EIB One 20ul syringe named Reagent Syringe One 4VM01 manifold named Reagent Manifold with one valve connected at position 1 Rename the 20u syringe to Reagent Syringe and the 4VM01 manifold to Reagent Manifold by right clicking on the device icon in the Devices Window and choose Rename In general the name may have a maximum of 16 characters and may not include lt lt or Names may include tabs and spaces but may not start or end with them Names are case sensitive The automated script is created as follows In the uDevices Window select Interfaces to display the Device Contro
70. witch valve to fill position SetValves 1 0 0 U WaitDone fill syringe with liquid from reservoir SetFlowRate 150 ul min MoveTo 80 ul WaitDone switch valve to dispense position SetValves 3 0 0 U WaitDone RegUpTo 2 kPa SetFlowRate 20 ul min MoveTo U ul WaitDone One potential issue with the above script is if the syringe reaches the end before the pressure reaches 2 kPa the script will never complete To account for this condition the last step Pressurize can be changed to include a loop that looks to see whether the syringe has finished If the syringe finishes before the pressure is reached the valve switches back to the reservoir to refill the syringe and then the device is pressurized again This script is shown below 51 Pressure Regulation Example Script with auto re fill of syringe if pressure is not reached OPS O I UPS x 4AM x AVM SWLeech ELLI 4VM 4VM ELEL SPOOL SPoUL SPOOL Switch Dispense AVM AVM Pressurize UPO SPS01 SES 0A Check Loop TEE lt GoOto gt UPS lt Goto gt lt Pause gt lt Goto gt E H OO wL uPSO C00 kPa 4AMO1 AVMO1 E switch valve to fill position SetValves 1 0 0 U WaitDone fill syringe with liquid from reservoir SetFlowRate 150 ul min MoveTo 80 ul WaitDone switch valve to dispense position SetValves 3 0 0 U WaitDone RegUpTo 100 kPa SetFlowRate 20 ul min MoveTo O ul 1fdone SWIECA Fat 1fdone
71. y RegBetween 70 130 SelectReading MPixelsO 1 3 0 RegDownTo 4000 APPENDIX A MENUS TOOLBARS WINDOW CONTROLS Menu Bar The menus contain the following functions File New Open Save or Print Edit Undo Cut Copy or Paste text while editing an automated process View Choose which uProcess windows and toolbars are displayed Actions Run Pause or Stop an automated process control hardware communications and update firmware and calibrations Click and drag the four dots at the left of the menu bar to tear it from the main window as a floating palette Click and drag the floating palette back into the main window to re dock it Toolbar Toolbar buttons provide one click shortcuts to common tasks Buttons are gray when those functions are not currently available a CE Ba GO E mm Ore Figure 28 uProcess Toolbar Buttons a New ctrl n Create a new automated process Open cirl o Open an existing automated process a Save ctrl s Save the current automated process cut ctrl x While editing an automated process delete selected text and place it on the clipboard 79 Copy ctrl c While editing an automated process copy selected text to the clipboard Ee Paste ctrl v While editing an automated process paste text from the clipboard pf T Print ctrl p Print the text of an automated process Y About uProcess Find the uProcess version number This window also contains links to the electronic version of this User

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