Leica Acquire User Manual
Contents
1. Image Size Select the captured image size from the available resolutions in the list Each camera may have several different sizes Format Select from TIFF 8 amp 16 Dit JPEG 8 bit only PNG 8 amp 16 bit Filename The saved image file name has an automatically incremented number added to the end of the name By default and if the name is blank this name is image 1 The increment number can be set or reset to any number Capturing an Image Once the image is satisfactory capture it by clicking the Capture button in the bottom right corner The image will be added to the Gallery and stored in the folder defined in the Folder options Imaging Tools Using Leica Acquire Imaging Tools mor OS BB oa 9 Tools Rotate Image Flip Image Delete These tools are available for manipulating the live image or any captured image All Images that appear in the Gallery may be edited with these tools apart from Exposure Check Toolbox The Toolbox contains mouse controlled tools to select actions for drawing a Cropping region White Balance Magnifying and Navigating around an image Selecting a tool changes the shape of the mouse cursor and function of the mouse and by doing so makes the tool active _ Arrow The Arrow tool turns off other mouse tools This is the default cursor _ Zoom This will enable you to Zoom In or Zoom Out of the image Zoom In Move the cursor over the live image and it2. System Requirements Supported Cameras Leica EC3 ICC50 ICC50 HD IC80 HD cameras Leica EZ4 D amp EZ4 HD stereo microscopes Computer System The minimum Mac computer specification is as follows e 1 GHz Intel Core2 Duo processor with 1 GB RAM e Graphics 256Mb RAM supporting 32 bit USB 2 0 High Speed port e CD ROM drive or internet connection for download Approximately 30 Mb of disk space for a full installation Further space is required for data and image storage Check free disk space regularly Operating System Supported Mac OS operating systems 10 5 Leopard or later Please use the latest updates Contact www apple com for updating your system 10 4 Tiger and earlier Mac OS versions are not supported Please Note Faster processors additional RAM memory and more advanced graphics adapters may improve the performance of Leica Acquire Running other programs at the same time may limit performance Leica Acquire User Manual Feedback For help with any problems please refer to the sections in Troubleshooting or Hints and Tips Also check the latest FAQ pages at www leica microsystems com education If you still encounter problems report any issues or feedback concerning this software to ez support leica microsvstems com Please describe the exact configuration you are working with and your software version including Software Version number Computer description brand name operating syste
3. adjusting and capturing the live image Capture Capture Camera Leica EZ4 HD Camera be Live Video Auto Mode 1024 x 768 Exposure A 34ms Sharpen Color C Auto Levels Auto Y Output 2 When viewing a captured or saved image this panel displays information about the selected image Review Name Strobilius 192 Format TIFF Dimensions 1024 x 768 RGB Date 31 08 2010 16 47 29 Author DG Copyright 010 LCD Description Safranine xa Overview Using Leica Acquire Image Viewer The right side and largest part of Acquire shows the Image Viewer In the Capture Panel the live image fills the Image Viewer as above In the Review Panel the Image Viewer displays the image selected from the thumbnails in the Image Gallery Imaging Tools JO Ba 4 9 Rotate Image Image Crop Delete These tools provide manipulation of live or acquired images Image Gallery Acquired images are displayed as thumbnails in a horizontal gallery along the bottom of the main Acquire window gr 1 E Td i 2 0003 2 0004 2 0005 2 0007 2 0008 E 5001 10 Leica Acquire User Manual Menu Bar Leica Acquire File Edit Image Window Help As with all other applications on the Mac OS Acquire displays a menu bar along the top of the screen Leica Acquire contains general Apple menu commands for the entire application such as hiding or quitting an
4. folder Leica Acquire 1 0 app For easier access to Acquire drag the icon to the Dock on the Desktop or create an alias and place the alias on the Desktop Leica Acquire 1 0 2 Double click the icon and Acquire will open in the design as shown overleaf Overview Using Leica Acquire Overall Design Leica Acquire File Edit Image Window Help E a E Vol dae TA a9 ys A l FEE Ey Capture El Review gt A a Su 2 h i x an i pm a ri di Camera Leica EZ4 HD Camera settings Reset Live Video Mode Exposure j 67ms Gain i Brightness eee 22 El Y Output Image Size 2048 x 1536 4 Format Filename Strobilus 197 B ne ur Di 5 eg a Tinta si e a LU k ia TE E A H1 7 Fia Lato quia y ie A Strobilius 194 Acquire shows camera controls on the left and the Image Viewer on the right as shown Acquire is divided into 5 main areas Acquire has a common Mac OS Menu Bar Control and information panels Image Viewer showing the live and captured images e Imaging Tools e Image Gallery Menu Bar You can vew the help files and version information and also select some image processing Go to the Menu Bar gt Leica Acquire File Edit Image Window Help Leica Acquire User Manual Control panel The control panel has two different modes 1 When viewing a live image this panel contains the camera controls for setting
5. ATA USB l Network b Software Bluetooth USE Host Controller Y USB High Speed Bus E74 HD Built in iSight E74 HD Product ID 0x3001 Vendor ID 0x1711 Version 0 00 Serial Number LEICADODOODO 1 Speed Up to 480 Mb sec Manufacturer LEICA Location ID Oxfd300000 Current Available m 500 Current Required mA O If USB High Speed Bus is displayed then the computer supports High Speed USB If you have already a High Speed USB device connected to a High Speed USB port the device Speed should read Up to 480 Mb sec Otherwise it is a standard speed USB device and or port Connecting the cameras Installation Connecting the camera or microscope Familiarise yourself with the USB cable provided and the connections on the microscope The rear of the microscope The USB cable mini USB plug standard USB plug Connect the power lead to the rear of the base of the microscope Do not switch on the power EZ4 D Connect the mini USB plug of the USB cable to the rear of the microscope head as shown connect the mini USB plug gt Connect the standard USB plug to a free High Speed USB port on the computer Switch on the power to the microscope EZ4 D The Leica Stereo microscope or camera should now be ready for you to start Acquire See how to use Acquire gt Leica Acquire User Manual Overview Starting Acquire Leica Acquire can be started from the Leica Acquire icon in the application
6. Leica Acquire User Manual MICROSYSTEMS Leica Acquire User Manual All reasonable steps have been taken to ensure that this publication is correct and complete but should any user be in doubt about any detail clarification may be sought from Leica Microsystems or their accredited representative The information in this document is subject to change without notice and should not be construed as a commitment by Leica Microsystems Leica Microsystems accepts no responsibility for any errors that may appear in this document 2010 Leica Microsystems No part of this publication may be reproduced transmitted transcribed stored in any retrieval system or translated into any human or computer language by any means or in any form without the prior written permission of Leica Microsystems Ltd Apple Mac amp Macintosh are trademarks of Apple Computer Inc At Leica Microsystems we are continually striving to improve the standards of our manuals and would welcome customer feedback If you would like to comment on any aspect of this manual or our manuals in general please send email to LMIS manuals leica microsystems com Alternatively send a fax to 44 0 1223 412526 or write to Manual Feedback Marketing Department at the address below Please quote the title and date of the manual Date October 2010 Software Version 1 0 0 Leica Microsystems Cambridge Ltd PO Box 80 515 Coldhams Lane CB1 3YZ United Kingdom Te
7. RGB Date 15 10 2010 13 43 41 Author mo o Copyright MS 2010 Description tera 274 blk 34 Tools Rotate Image Flip Image DO S Strobilus206 Strobilus207 Es Acquired images are displayed as thumbnails in the expandable horizontal Gallery at the bottom of the main Acquire window An image selected in the Gallery is displayed in the viewing area on the right and the image description is shown in the information panel on the left There are also a number of tools below the displayed image for further processing The description is attached to each image and some items may be amended Image Information Name This is the name applied at the time of acquisition in the Output option in the Capture panel To rename an image type another filename into the box If it already exists the filename is rejected and the original name is established Format 22 Leica Acquire User Manual 23 This is the file type selected in the Output option in the Capture panel It is not editable Dimensions This is the size selected in Image Size in the Output option in the Capture panel It is not editable Date This is the date and time of the acquisition It is not editable Author You can type any name in this box Copyright You can type any name in this box Comments You can type any comments in here Long descriptions will automatically scroll beyond the end of the displayed panel Imag
8. Reveal in Finder This will show the file in the location on your computer in Finder Delete Deletes the selected image or images from the Gallery and your computer Undo Press Z keys to undo the above actions or select Undo from the Menu Bar 26 Leica Acquire User Manual 21 Troubleshooting The live image window is completely gray Check that the USB connection from the microscope to the computer is connected securely Check that the microscope power cable is connected securely and the power is on at the mains and at the microscope Check that Acquire has been installed correctly and that the cameras are visible in the Computer Hardware Overview The live image window is completely black Check the light conditions and the microscope light settings Ensure that light reaches the camera The incident lights are faulty or covered by an obstacle The transmitted light box on the microscope is faulty or has been covered by opaque materials Exposure or gain is too low increase exposure or gain or both Gamma is set too high decrease gamma Auto Brightness is selected but the brighiness level is too low increase the brightness level The live image is too dark Exposure or gain is too low increase exposure or gain or both Gamma is set too high decrease gamma Auto Brightness is selected but the brighiness level is too low increase the brightness level Check the microscope and lighting settings The
9. d also general Window menu commands for minimising and restoring Leica Acquire About Acquire displays the application version information Preferences 38 General has the option to open the image in another application after capture ASA General O a de General Camera M After saving open image of EE Preview app Choose other lt Camera displays the active camera name and serial number and firmware version Uninstall A list of all installed Acquire components will be displayed including camera drivers libraries and the application To uninstall Acquire completely make sure all components are ticked You must disconnect all cameras before starting the uninstall process Quit Acquire 3 Q closes Acquire File New Image will capture an image W will Close Acquire like Q Edit Image Window Help Delete Select All Special Characters ST Delete Delete Move to Trash one or more selected image in the Gallery This will delete the file from your computer 11 Menu Bar Using Leica Acquire Undo The last processing action performed on an image can be Undone 36 2 Select All Select All selects all images in the Gallery Image Window H Rotate Left Rotate Right Flip Horizontal Flip Vertical Crop Grayscale invert Reveal in Finder Rotate Left turns the image in a counter or anti clockwise direction by 90 Right turns the i
10. easing Gain brightens the image without increasing the exposure time However high gain values can reduce image quality The minimum value of 1x is to the left and different cameras have different maximum values to the right You can write values directly in the edit box Auto When checked this will enable automatic brightness and you can adjust the level with the Brightness slider Color You can adjust the image Color and Saturation and select automatic White Balance Y Color Auto Saturation gt 100 Saturation The Saturation slider sets the color intensity of the image It can be adjusted to the left so that the image appears as monochrome black amp white and to the right for falsely bright colors The default is 100 The range of the slider is from O 200 but you can write directly in the edit box these and any values greater that 200 Color The color wheel is used for fine tuning the color balance Depending on the light source subject matter and camera characteristics fine correction of the image may be necessary even after a white balance Drag the central dot in the color wheel towards the color you require White Balance White Balance corrects the live image without any color bias neutral lt can be applied to the whole image or to a specific region The term white balance is not strictly correct neutral balance would be more accurate but as the term is used worldwide we will continue
11. er exposed image as blue and red regions respectively You can also adjust these regions by dragging the histogram black and white levels The histogram is marked with corresponding pale blue and red colors Y Levels Auto 20 Leica Acquire User Manual Delete Delete Delete Move to Trash one or more selected image in the Gallery This will delete the file s ffom your computer Zoom Slider amp Inset Zooming in and out can also be achieved with the Zoom Slider located at the bottom right corner of the application window just below the Capture button Move the slider to the left to Zoom Out and to the right to Zoom In It performs the same function as the Zoom tool above but with a visible indicator for minimum and maximum positions Once the image is zoomed in a Zoom Inset shows in the bottom right corner of the live image A rectangle inside the inset shows how much of the image is visible in the Image Viewer A green rectangle shows the image is viewed at below 1 1 and a red rectangle shows the image is viewed at over 1 1 Undo All of the processing actions performed on an image can be undone with 6 Z or from Undo on the menu bar 21 Viewing Captured Images Using Leica Acquire Viewing Captured Images The Review Panel enables you to view captured or stored images and add or change the image name or descriptions Review Mame Strobilus202 Format JPEG Dimensions 2048 x 1536
12. in the edit boxes use arrow keys for precision These controls remain active until you move them again or check Auto To obtain the best image these contrast indicators for minimum and maximum brightness levels should be aligned with the limits of the brightness distribution The closer the indicators are together the higher the image contrast The default minimum is 0 and default maximum is 255 Gamma Gamma adjusts the contrast range and how it is applied to an image For a gamma value of 1 0 contrast is applied evenly linearly across the whole light intensity range However dark areas for example may appear too dark to view easily and by adjusting the gamma to below 1 0 to the left the detail becomes more visible The image becomes lighter but also duller Conversely increasing the gamma above 1 0 to the right makes the image darker but also more vibrant Gamma is adjusted by dragging the middle triangle indicator The default is 1 0 As the gamma value is calculated as a factor of the minimum and maximum gray levels the gamma triangle position moves according to the position of the contrast indicators Auto When checked optimum gamma black and white levels are automatically calculated from the current brightness distribution 16 Leica Acquire User Manual 17 Output The captured image type and name can be selected here v Output Image Size 2048 x 1536 Format JPEG Filename Strobilus
13. ing Tools Toolbox The Toolbox contains mouse controlled tools to select actions for cropping magnifying and navigating around an image highlighted in the Gallery Button Tools These tools rotate flip and crop the image The operations of all these tools are shown in Imaging Tools Image Gallery Using Leica Acquire Image Gallery Acquired images are displayed as thumbnails in an expandable horizontal Gallery along the bottom of the main Capture window Image Types The Gallery will display and open any files on your computer with a file type of Jpeg or jpg Tiff or tif and Png Acquire will ignore other file types It will also display and open files on another networked computer if you have access to these files Access to the Gallery Di The Gallery can be opened or closed by toggling Show Hide at the bottom of the control panel The Gallery is accessible when viewing live or acquired images Folder options The current Gallery folder is shown in Folder The default folder is located in Pictures Acquire where is the current login username Click Folder to change the location or create a new folder by selecting Other Folder from the list of options Make sure you have write access permissions to capture images to the folder you select Images are captured and saved to the current gallery folder active at the time of capture Once the Gallery is open the size and number of visible
14. ing the Live Image Using Leica Acquire Camera Leica EZ4 HD Camera HH Settings Reset H Live Video 1 Auto Mode 1024 x 768 A Exposure m 3A MS B Gain EA 1 4x E Sharpen Color Auto Levels Auto Y Output Camera configuration Camera Normally Acquire will startup and show a live image with the active camera automatically If you have more than one camera connected to the computer you can select which camera is to be active Settings Once you have defined the best camera settings or configuration for your environment you can Save them and apply them at any time This is not only useful for different working and lighting conditions but can also be used to reproduce user specific working conditions You can Delete any saved setting and also Reset a camera to its default configuration Live Video This contains the controls for adjusting the basic brightness of the live image either manually or in automatic mode Mode Select the largest resolution for a better quality live image and the smallest for a lower resolution but with faster frame rates Exposure Increasing Exposure time will make the image lighter However as the exposure increases the live image refresh frame rates will become slower Minimum exposures are to the left and maximum to the right You can write values directly in the edit box 14 Leica Acquire User Manual Gain Gain is used to amplify the image signal Incr
15. ion of images in the folders on your computer create sub folders rather than having one large folder for the Gallery Mouse Functions Most Macs are traditionally operated with a single button mouse but can also be used with a two button mouse which Leica Acquire takes advantage of 1 button mouse Normal functionality is by clicking the mouse Alternative functions are accessible by holding Ctrl Control key mouse click 2 button mouse Normal functionality is by clicking the left mouse button Alternative functions are accessible by clicking the right mouse button as well as by holding Ctrl key either mouse click 28
16. l 44 0 1223 401824 Fax 44 0 1223 412526 email ez support leica microsystems com web www leica microsystems com Table of Contents INIFOGUCHNO q ERRO SRD UA RAS RR RR DO AS NI RR DO AS RR 1 What IS Leica Acqua gt iai o cpa pa delie i lie leleine iii ii i ei 1 Sa A 2 Fecdback cile ce eil Rita Co iii gp 3 installation lella eil 4 Installing Lelca AGquire ceco alal LL LL LL i 4 Connecting the camera or microscope cin 5 Conneciing he Gameras cc LDL ALL LINE DIL Lai 6 Using Leica ACQUIFE sca 7 A 0 A ell ei iii i ide 7 Mena elle ili alleno a e i 11 Controlling the Live Mage e et 13 ImagingrTools aana a ns ie ee Aa 18 WIE WING Captured MATES ria a a a a aeaa ieri 22 Image Galery mass e AS a 24 TroUblesno0O0 IN a A CREA ER ER EE RR lalla 27 Troubleshootind cla ii 27 AS cesello ua 28 Leica Acquire User Manual What is Leica Acquire Leica Acquire is a software environment which allows the acquisition analysis and processing of high quality digital images using Leica cameras and stereo microscopes on an Apple Mac Leica Acquire comes with the latest Leica educational cameras and stereo microscopes with integral digital camera and provides the basic software for the camera configuration and control as well as image acquisition Captured images can be viewed in a gallery saved to specified file locations and can have additional processing applied to them System Requirements Introduction
17. live image is not in focus Check the microscope settings and follow the instructions in the microscope operating manual Compare the focus through the eye piece and compare with the image within Leica Acquire lt is possible that they are not identical As a temporary work around focus using the live image within Leica Acquire Contact ez support Check the Zoom dial on the microscope The focus is automatically adjusted when zooming in or out It is possible this may be defective Contact ez support Poor image quality Check microscope light settings Check exposure and gain settings to avoid under or over exposure Make sure the eyepiece fits snugly around the eye so no stray light enters the eyepiece Cover the eyepiece to external light if it is not being used Check the gain setting An Increase in Gain will increase the electronic noise of the microscope camera If you reduce Gain you should increase the exposure to compensate Hints and Tips Troubleshooting Hints and Tips Navigation within Leica Acquire Navigate check boxes amp edit boxes within the controls panels using the Tab key Slow live image performance Set Exposure to as short a time as possible to obtain a correct image Increase Gain to compensate if necessary This will improve camera response and performance Set the image Live Format to a smaller resolution for a faster live image Browsing the images on your computer In order to speed up the navigat
18. ll become slightly grayed out The area that you wish to keep inside the cropped region will remain at normal viewing brightness A To move the region you wish to crop move the mouse inside the rectangle to show a Hand cursor Click and drag the rectangle to a different position To resize the region grab an edge or corner and drag to enlarge or reduce the rectangle Deactivate the cropping region by clicking again with the crop cursor showing anywhere in the grayed out area outside the rectangle To crop the image to this region use the Crop Image button Button Tools These tools rotate flip and crop the image ae Pie DI S Rotate Image Rotate the whole image right clockwise or left anti or counter clockwise by 90 You can also apply this to multiple captured images by selecting more that one image in the Gallery 63 Flip Image Flip the whole image horizontally or vertically You can also apply this to multiple captured images by selecting more than one image in the Gallery Crop Image After drawing a crop region from the Toolbox the Crop Image button becomes active Click to crop the image Imaging Tools Using Leica Acquire J F 4 J Ri A ii Si P 3 a You can only crop one image at a time E Exposure Check live image only Sometimes it is difficult to know when the live image is under or over exposed When active E Exposure Check indicates those parts of the under and ov
19. m RAM language etc Microscope or camera description Description of the exact steps that produce the error Screenshot of any error message Installing Leica Acquire Installation Installing Leica Acquire Make sure there are no cameras or microscopes connected to your computer Identify the version of your Mac operating system by opening About this Mac from the Apple menu If necessary update your operating system to meet the specification in the system requirements section 000 About This Mac E nre r Mac OS X Version 10 5 8 Software Update Processor 2 16 GHz Intel Core Duo Memory 1 GB 667 MHz DDR2 SDRAM Startup Disk Macintosh HD More Info TM amp 1983 2009 Apple Inc All Rights Reserved Installing the software From the CD or downloaded disk image double click the icon to install Leica Acquire A Before starting the installation you may have to enter the administrator password to make changes to your hard disk Click Unlock to make changes enter the password and click OK Click the lock to malka change How enter the password and click on OR Patente O Fon al a creia rimi amd pa A As be cero be for poa fi im Hires ae Pores or pee PETT an E Double click Install Leica Acquire package and follow the instructions The Release Notes and User Manual also can be viewed here gt E Leica Acquire Release E Leica Acquire User Notes pdf qu Ma
20. mage in a clockwise direction by 90 Flip Horizontal flips the image from left to right and vice versa Vertical flips the image from top to bottom and vice versa Crop Click to crop the image after first drawing a region using the Toolbox Crop Region Tool Grayscale Converts a color image to a grayscale or monochrome black amp white image Invert Creates a negative picture from the image Reveal in Finder This will show the file in the location on your computer in Finder Window Fullscreen F displays the live or saved image across the whole of monitor display screen without any controls being visible You can toggle 36 F at any time Help Acquire Help opens this Help file 86 12 Leica Acquire User Manual Controlling the Live Image The live image can be viewed and adjusted in the Capture Panel Camera Leica EZ4 HD Camera E Settings Reset Y Live Video C Auto Mode 1024x768 Exposure ne 67m5 ll Gain AA 2 0x B Brightness m 22 2 b Levels C Auto v Output Image Size 2048 x 1536 H Filename Strobilus 197 Camera controls are on the left the live image is on the right Camera Controls Camera controls are divided into several main areas Camera configuration Adjusting basic brightness of the Live image White amp Color Balance Sharpening the image Histogram Selecting Output options for acquisition Image Capture 13 Controll
21. nual pdf Install Leica Acquire pkg After installation you can connect your camera or microscope to the computer Leica Acquire User Manual Connections on the Computer USB The Leica educational cameras and stereo microscopes use a High Speed USB USB 2 0 connection to the computer Please make sure both your computer and Mac Operating System support High Speed USB USB 2 0 If your computer only supports standard USB USB 1 or 1 1 please update your hardware Does your computer support USB The first step for proper USB connectivity is to make sure your computer supports USB and is enabled If in doubt consult your computer system administrator or computer supplier A USB socket or port on your computer looks like this with a USB symbol Which version of USB do you have If your computer and Mac OS already supports USB but you are not sure if it is a High Speed USB you can check which version you have in the following way Open the About this Mac panel from the Apple menu and click More Info This will display your computer system properties Expand Hardware and Click USB Contents USB Device Tree Y Hardware Y USB Bus ATA Apple Internal Keyboard Trackpad Audio Built In DELL Laser Mouse Bluetooth USB Bus Diagnostics 7 USB Bus Disc Burning IR Receiver Fibre Channel Y USB Bus FireWire Graphics Displays Hardware RAID Memory PC Cards PCI Cards Parallel SCSI Power Printers SAS Serial
22. thumbnails can be controlled by dragging the bottom of the gallery up or down 24 Leica Acquire User Manual 25 The number of images in the Gallery folder is displayed below the image viewer You can select one or more thumbnails and the number of images selected will be shown as well as the free space available on the computer or volume For example 4 images have been highlighted out of 24 that are visible to Acquire The amount of free space available on this computer is 31 7 Gb Image Options Gallery actions will permanently affect the image file on your computer and not just in Acquire Each of these actions may be applied to more than one image at a time This means that the action will be applied to all thumbnails highlighted in the Gallery and not just the image displayed in the Image Viewer Rotate Left Rotate Right Flip Horizontal Flip Vertical Crop Grayscale Invert Reveal in Finder Delete Rotate Rotate Left turns one or more selected image in a counter or anti clockwise direction by 90 Rotate Right turns one or more selected image in a clockwise direction by 90 Flip Flip Horizontal flips the image from left to right and vice versa Flip Vertical flips the image from top to bottom and vice versa Grayscale Image Gallery Using Leica Acquire Converts a color image to a grayscale or monochrome black amp white image Invert Creates a negative picture from the image
23. to use it here When Auto is checked White Balance is continuously applied to the whole of the live image To make a White Balance at a particular point or a region drawn on the live image select the White Balance eyedropper in the Toolbox Sharpen Apply Sharpening to Live and Captured images Move the slider to the left for minimum and to the right for maximum sharpening 15 Controlling the Live Image Using Leica Acquire Levels The live image Histogram displays a visual representation of the current light levels as seen by the camera Y Levels Auto CS o io fas It shows the relative distribution of pixels by tonal value from the darkest elements indicated in the grayscale bar on the left hand side to the brightest on the right A peak represents many pixels in that density range A well illuminated image should contain all elements from dark to light While automatic exposure offers the best overall image quality for most acquisition purposes you may wish to make some adjustments The histogram can be used to control brightness and contrast of an image and an understanding the histogram is therefore important to obtain the best captured images You can adjust the minimum brightness gray level or black point or level by dragging the left most triangle indicator below the grayscale bar and maximum brightness gray level or white point or level by dragging the right most triangle You can also alter these values
24. will change to A Click one or more times on the live image to zoom in by fixed amounts Zoom Out Repeat as for Zoom In but press the alt key at the same time and the mouse will appear as A Click on the live image one or more times to zoom out by fixed amounts Alternatively you can click in the live image and scroll the mouse up or down to zoom in or out smoothly See also the Zoom Slider Hand The Hand tool enables you to navigate around the whole of an image when it is zoomed in lt is grayed out when fully zoomed out but becomes available once the image is Zoomed in The mouse cursor turns to a hand Click in the live image with the Hand cursor and drag the mouse anywhere in the image White Balance eyedropper For a White Balance at a particular point click on the live image A cross wire will appear on the live image and remain at this point until White Balance is de selected When the eyedropper is moved within the live image r g b pixel values for the current cursor position are displayed below the live image 18 Leica Acquire User Manual 19 For a region ROI draw a rectangle in the live image with the mouse As the mouse is released the White Balance is calculated over the selected region but applied to the whole image To apply the White Balance to the whole image click Auto in the Color panel ES Crop Region Draw a rectangle with this tool The area you wish to crop or cut away wi
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