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Mounting a Coolpix 5000 onto a Microscope Reference Sheet
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1. com 1 800 52 NIKON
2. Nikon UMENTS INC Mounting a Coolpix 5000 onto a Microscope Reference Sheet Please refer to the Coolpix 5000 User s Manual for page numbers indicated below Mounting the Coolpix 5000 to a microscope trinocular photo tube or beamsplitter With the camera power off open the card slot cover and make sure a CompactFlash Card or IBM Microdrive is properly inserted into the camera See p 36 Slowly thread the UR E6 Step Down Adapter to the camera Carefully thread the MDC A Relay Lens onto the UR E6 Step Down Adapter Likewise thread the Cmount onto the MDC Relay Lens securely Note that over tightening the adapter may damage threads of both adapter and apparatus Attach the C mount adapter onto the microscope s trinocular or beamsplitter port Secure the C mount into place Adjust the LCD screen to the desired viewing position and angle Note Use a 1x C mount e g Nikon ISO 1x C mount 83043 to ensure the maximum field of view EH 52 AC Adapter is recommended to eliminate battery consumption Mounting the Coolpix 5000 into a 30mm eyepiece tube 1 With the camera power off open the card slot cover and make sure a CompactFlash Card or IBM Microdrive is properly inserted into the camera See p 36 UR E6 Step Down Slowly thread the UR E6 Step Down Adapter to the camera Adapter Loosen the three setscrews of outer sleeve on the MDC A Relay Lens and remove the Cover Ring Carefully thread the MDC A relay lens to
3. t image An image without black cut off corners or circular images is generally possible at mid range of the zoom setting Optional Settings in Menu Selection Above the LCD display press center button above Menu for the Shooting Menu for camera settings available only in the P S A and M mode and only when the camera is in shooting mode The following are recommended options for photomicrography White Balance The default setting is Auto Select a white balance setting that matches the microscope light source incandescent for tungsten or halogen illumination Select White Bal Preset for unusual lighting conditions See p 102 104 Image Quality Image quality settings other than H are compressed as they are saved into memory Compression selectively reduces the quality of an image Certain illumination techniques such as DIC phase contrast OCC etc may require special brightness and control adjustments for best results See p 72 73 Metering The default setting is Matrix Setting it to Spot on a targeted sample with a very dark or a very bright background would ensure most appropriate exposure See p 105 Continuous The default setting is S single shutter release Using other settings continuous sequence of pictures may be taken while the shutter release button is held down See p 108 109 Saturation Control The Black amp White setting will enhance black and white samples See p 115 Nikon Instruments Inc Nikonusa
4. the UR E6 Step Down Adapter Note that over tightening the adapter may damage threads of both adapter and apparatus Remove an eyepiece from the microscope and insert the Cover Ring available end of the MDC A Relay Lens adapter as replacement Adjust the LCD screen to the desired position and angle Setscrew x3 The weight of the camera may cause its positioning to become unstable Note Nikon Eclipse and Nikon Stereomicroscopes are 30mm in diameter Other Nikon models amp microScopes from other manufacturers may vary The EH 52 AC Adapter is recommended to eliminate battery consumption This reference sheet is to be used in addition to the Coolpix 5000 User s Manual Nikon INSTRUMENTS INC Coolpix 5000 Camera Settings for Microscopy 1 Turn the camera on and select the infinity mode by pressing the Manual Focus MF button until the icon with two mountain peaks is displayed in the upper right of the monitor display Note that the flash turns off automatically when focus is set to infinity See p 77 Hold down the MODE button and simultaneously turn the COMMAND DIAL to select A Aperture Priority Auto mode The camera will set shutter soeed automatically for best results Rotate the COMMAND DIAL to set the F value to the lowest possible f stop number The approximate range value F2 5 F4 0 is dependent on the zoom setting Use the zoom buttons W T to fill the field of view Use the LCD screen to determine bes
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