injectors - Harvard Apparatus
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1. EC1 69 0150 EC1 69 0151 EC1 69 0164 and EC1 69 0163 Pulled Pipettes for use with EC1 69 0152 EC1 69 0153 EC1 69 0155 Output Tubing with EC1 69 0152 EC1 69 0153 EC1 69 0150 EC1 69 0151 Connector and 4 5 ml Vial for use with 9 Nanoject Il Auto Nanoliter Injector Previous Micromanipulator and Stand sold separately Next Section Table of Contents Main LEL Coi Contents Contact Us e Lower volume 2 3 nl capability e Positive displacement technology e 2 3 to 69 0 nl range of volumes e Minimal vibration e New improved high torque motor e Membrane key pad e Improved micropipette holder e Two speed injection fill rates e Auto Home capability The Nanoject Il is the latest development in the Nanoject family of microinjectors One unique features of the Nanoject Il is the use of a high torque motor to provide smooth operation resulting in substantially reduced tip movement A new collect configuration holds the micropipette more securely thereby reducing air infiltration and oil leakage The two speed fill inject mode allows the user to fill and inject at half speed facilitating the use of smaller micropipettes The handling of more viscous samples is also improved All of the above factors enhance the precise delivery of sample Volumes for the Nanoject II range from 2 3 nl to 69 nl The smaller tips and smaller injection volumes of the Nanoject II allow users to perfo2. Next Section Table of Contents Main LEL CK Contents Contact Products EC1 65 Pico Injeci EC1 69 0153 8 Channel Pressure Injection System see page 8 272 FF ATED EE j NANOJECT Hi D Micromanipulator Es g J u and Stand sold separately see io er Ye EC1 69 0105 Screw Actuated Air Syringe see page 11 Page No lt Introduction to Microinjection Previous The use of Glass Needle Fine Glass Microcapillary Pipette based Next techniques for intracellular extracellular microinjection and perfusion has become a popular procedure in numerous areas of experimental biology research e g In vitro fertilization transgenics etc These techniques can best be described as micro surgical procedures that are conducted on a single cell using either a single Warner and Harvard Apparatus Clark Capillary Glass or multiple barrel glass micropipette a precision positioning device micromanipulator and a microinjector or microperfusor The micropipettes used in these procedures are formed using a Pipette Puller The capillary glass tubing is heated to its softening point and pulled to create the proper size tip diameter and taper for the desired application The small tip diameters as low as 0 2 um of these micropipettes combined with the high precision of the Micromanipulator and Microinjection Perfusion Apparatus allows for precise and accurate delivery This precision allows for accurate
3. 05 to 10 p s i regulated 0 to 24 in Hg regulated 0 to 30 in of water regulated 0 to 30 in of water regulated 0 02 p s i 0 05 p s i for pressure 0 2 in for vacuum lt 2 in H 0 10 msec to 327 67 sec 10 msec resolution for whole range 1 to 255 16 sequences 32 steps for each sequence DB9 connector for remote key pad foot switch jack and BNC for negative TTL trigger jack connector for injection foot switch Eight BNCs for injection signal on output port 1 to 4 100 120 or 220 VAC 35 W Order EC1 69 0152 EC1 69 0153 Model PM 4 PM 8 Product 4 Channel Pressure Injection System 8 Channel Pressure Injection System Options and Accessories EC1 69 0154 EC1 69 0155 EC1 69 0156 EC1 69 0157 EC1 69 0158 EC1 69 0160 EC1 69 0163 EC1 69 0164 EC1 69 0165 EC1 69 0166 EC1 69 0167 IP 1 OP 1 OP 2 FSW PM KP RK 2 PP 7 PP 4 HOLD 1 HOLD 4 HOLD 7 Input Tubing with Connector Output Tubing with Connector and 4 5 ml Vial Output Tubing with Connector only Footswitch Remote Key Pad Rack Mounting Kit with Handles for PM 4 8 Pulled Seven Barrel Pipette each Pulled Four Barrel Pipette each Holder for Single Barrel Pipette Holder for Four Barrel Pipette Holder for Seven Barrel Pipette EC1 69 0156 Output Tubing with Connector for use with EC1 69 0152 EC1 69 0153 EC1 69 0150 EC1 69 0151 EC1 69 0154 Input Tubing with Connector for use with EC1 69 0152 EC1 69 0153
4. an input and output hose handle power cord and instruction manual The plus model is supplied with all of the same parts as the basic model but also includes a Footswitch Pipette Holder and Input Hose Adapter Harvard Apparatus phone 508 893 8999 e toll free U S 800 272 2775 fax 508 429 5732 www harvardapparatus com Specifications Input Gas Pressure Injection Pressure Balance Pressure Clearing Pressure Injection Time Pressure Display Duration Mode Trigger Mode Pressure Readout Line Voltage Power Usage Foot Switch es Accessories Supplied Weight Dimensions H x W x D Order EC1 65 0005 PLI 90 PLUS Model EC1 65 0004 PLI 90 BASIC 70 to 105 p s i 480 to 720 kPa 0 2 to 60 p s i 413 kPa regulated multi turn control 0 1 to 3 5 p s i 68 9 kPa regulated multi turn control other ranges available upon request Input gas pressure unregulated 0 01 to 0 99 sec in 10 msec steps 1 to 99 sec in 1 sec steps Digital three and a half digits Internally timed or externally triggered Foot or panel switch Inject balance clear output port 100 110 220 240 VAC 220 W Optional inject and gating Input hose output hose and power cord 6 8 kg 15 Ib 11 x 38 x 25 5 x cm 5 x 15 x 10 in Product Pico Injector with Injection Balance and Clear Pressures Includes Input Hose PLI IHN and Output Hose PLI OHN Handle Power Cord and Inustruction Manual Applications Basic setups tha
5. cell Whether you need to do large Injections into capillaries or very small injections into mammalian nuclei the PLI 100 is well suited for your experiment The PLI 100 has become a favorite of prestigious national microinjection workshops like Cold Spring Harbor Laboratories and other researchers worldwide Other companies have tried to design similar systems but the PLI 100 remains unparalleled in terms of ease of use durability precision and cost Researchers Say The PLI 100 is very robust in constant use in our lab Most importantly the PLI 100 valves prevent cytosol and yolk backflow into my pipette after oocyte injection Its very easy to control Harvard Apparatus phone 508 893 8999 e toll free U S 800 272 2775 fax 508 429 5732 www harvardapparatus com FETE PE The PLI 100 is heavily used on a daily basis and works great I like the fact that can use the PLI 100 with TTL pulses It works great in conjunction with my imaging system The balance pressure is a good option My injection pipettes rarely clog Easy to Use Inject with the touch of a button or a tap of the foot switch it s as easy as that Reproducible Pressure to the pipette is controlled precisely through a multi turn regulator and is reported digitally for easy repeat Injection time is digitally controlled in 10 msec steps between 0 01 to 0 99 seconds and in 1 second steps betwe
6. reuse e Manual control or automatic sequential step cycle operation e Front panel display for pressures and programming information The PM 4 and PM 8 programmable 4 and 8 Channel Pressure Injector Systems are designed for one to eight channel intracellular injection and extracellular perfusion These microinjection and perfusion engines are especially designed for pharmacological drug testing molecular biological DNA RNA transferring intracytoplasmic sperm injection and cell electrophysiological applications Combining an advanced micro controller with precision pneumatic components these systems can simultaneously control up to eight injection micropipettes or eight perfusion vials one cell holding pipette and one drain pipette The four PM 4 or eight PM 8 injection perfusion output ports can be controlled separately or combined together to perform actions such as injection capillary action balancing suction or clear up The cell holding output port can produce an adjusted gentle suction to hold a cell eject a pressure to push a cell or completely clear the holding pipette Another drain output port can simultaneously produce an adjusted suction to drain and clear out liquid before the next channel micro perfusion The PM 4 8 can deliver different quantity agents and drugs from picoliters to continuous perfusion All output pressures and vacuums can be regulated and can be displayed real time on the front panel display Previou
7. when no separate balance pressure is offered by an injection device is avoided Fill Hold There are two built in vacuum generators to fill a micropipette from the tip and to hold suspended cells Filling the pipette from the tip is easier than back filling Suspended cells can be held with a second holding pipette The holding vacuum s range accommodates most cell types Clear A high pressure pulse can be used for clearing a pipette should it happen to clog This is particularly useful when working with pipettes sized for smaller volumes Electrical Connectors BNC type connectors are available at the front panel to ease integrating the Pico Injector with other equipment Synchronization of injections to other stimulations or recordings is therefore possible Selection Guidelines The PLI 100 is available in three packages Plus Basic and Deluxe The basic unit is supplied with an input hose output hose holding hose power cord and instruction manual The plus unit also includes a foot switch pipette holder and input hose adapter The deluxe unit includes all the previously mentioned accessories plus an additional foot switch and one pipette holder Accessories Available accessories for the PLI 100 Injection Systems are located on page Specifications 70 to 105 p s i 480 to 720 kPa 0 2 to 60 p s i 413 kPa regulated multi turn control Input Gas Pressure Injection Pressure 0 1 to 3 5 p s i 68 9 kPa reg
8. 1 69 0136 Standard O Ring EC1 69 0137 Special Flared O Ring EC1 69 0138 Replacement Wire Plunger EC1 69 0139 Needle 30 ga x 51 mm 2 in Accessories Must be ordered separately EC1 69 0140 Footswitch EC1 69 0141 Support Base EC1 69 0142 Universal Mounting Adapter see above photo EC1 3 000 034 International Wall Adapter EC1 3 000 033 US Wall Adapter Harvard Apparatus phone 508 893 8999 e toll free U S 800 272 2775 fax 508 429 5732 www harvardapparatus com 10 Previous Next Section Table of Contents Main LEL CK Contents Screw Actuated Syringes e For air assisted microinjection and microaspiration e Uncomplicated and easy to use e Accurate e Sensitive control e High suction pressure Screw Actuated Air Syringes These air assisted microinjection microaspiration syringes provide the user with sensitive control and an extremely low dead air volume The capacity of the syringe is 10 ml When required the syringes can be used to generate high pressure or suction The Screw Actuated Syringe is a small compact unit incorporated into a heavy circular base Its convenient dimensions enable it to be ergonomically positioned next to the control lever of a micromanipulator to facilitate single handed operation of both instruments The syringe is connected by hard polyethylene tubing to a micropipette which in turn may be fitted to a micromanipulator or positioning device Suction injection is obta
9. 2 www harvardapparatus com Previous Section Table of Contents Main LEL CK Contents Contact Us PM 3 and PM 4 8 and 4 Channel Pressure Injection Systems continued EC1 69 0152 4 Channel Pressure Injection System Below are the components supplied with each of the Pressure Injection Systems Pressure Injection System PM 4 PM 8 Order Product Oty Oty EC1 69 0154 P1 Input Tubing with Connector 1 1 EC1 69 0156 OP2 Output Tubing with 6 10 Connector Only EC1 69 0157 FSW Foot Switch 1 1 EC1 69 0164 PP 4 Pulled 4 Barrel Pipette 1 EC1 69 0166 HOLD4 Holder for 4 Barrel Pipette 1 EC1 69 0163 PP 7 Pulled 7 Barrel Pipette 1 EC1 69 0167 HOLD7 Holder for 7 Barrel Pipette 1 Power Cord 1 1 User s Manual 1 1 Specifications Output Channels Input Gas Pressure Clearing Pressure Clear Hold Pressure Injection Pressure Balance Pressure Fill Vacuum Hold Vacuum Drain Vacuum Repeatability Display Accuracy Timer Setting Range Counter Setting Range Savable Sequences Programmable Steps Remote Control Ports Signal Output Ports Power Consumption Harvard Apparatus phone 508 893 8999 e toll free U S 800 272 2775 fax 508 429 5732 www harvardapparatus com Four PM 4 or eight PM 8 injection perfusion outputs one synchronized drain out and one cell hold channel 30 to 100 p s i Same as input pressure 0 to 3 p s i regulated 0 05 to 60 p s i regulated 0
10. Holder 65 0013 and Input Hose Adapter EC1 65 0003 PLI 100 Deluxe PLI 100 Deluxe Pico Injector with Injection Balance Clear Filling and Holding Pressures Comes with Input Hose Output Hose Holding Hose Power Cord Instruction Manual Two Foot Switches Two Pipette Holders and Input Hose Adapter EC1 65 0002 PLI 100 Plus Harvard Apparatus phone 508 893 8999 e toll free U S 800 272 2775 fax 508 429 5732 www harvardapparatus com 5 Previous Next Section Table of Contents Main LEL CK Contents Contact Us PLI 90 Medical Systems Pico Injector EC1 65 0004 PLI 90 Pico Injector e 3 pressures inject balance and clearing e Eliminates backflow into pipette after injection e Reproducibly delivers femtoliters to microliters e Easy to use e Economical e Precise The PLI 90 Pico Injector like the reliable PLI 100 Pico Injector controls the precise and reproducible regulation of injection pressure and time Simplicity Because the PLI 90 features only the injection balance and clearing pressures it is a lower cost alternative to the PLI 100 This simplicity makes it even easier to use than the PLI 100 Selection Guidelines The PLI 90 is ideal for the user who does not require vacuum for filling pipette barrels or if using a holding pipette for holding cells is not essential to your application The PLI 90 is available in two packages Basic and Plus The basic model includes
11. and repeatable injections down to the sub picoliter liter range into or around various types and sizes of cells with accuracy to 0 1 micron The process of extruding substances through these micropipettes is accomplished through the use of either direct Harvard Apparatus Medical Systems Picoliter Injectors hydrostatic pressure Pressure Injection or by moving charged ions that are the result of an applied electric field lontophoresis without the use of hydraulic flow Section Table of Contents Main LEL Coi Contents Contact Us Applications of microinjection range from assisted In vitro cell fertilization techniques to the transport of molecular and cellular elements These substances are typically injected into the cell to manipulate and or monitor the fundamental biochemistry of a specific living cell Substance that can be injected include cellular organelles kinases histochemical markers such as horseradish peroxidase or lucifer yellow proteins metabolites microbeads ions antibodies genes molecular biology mRNA and DNA etc The precise delivery microperfusion of small volumes picoliter to milliliter of various agents and drugs to a cell or group of cells for Harvard Apparatus Medical Systems NeuroPhore visit oe l www harvardapparatus com for more information applications such as pharmacological drug testing can also be accomplished using these techniques In order for the researcher to conduct any
12. ator or similar instrument The 1 ml Hamilton syringe can be easily exchanged for any other Hamilton 1700 syringe series including the 1000 500 200 100 and 50 ul sizes visit www harvardapparatus com for complete details Order Product EC1 69 0107 Screw Actuated Micrometer Driven Hamilton Syringe Harvard Apparatus phone 508 893 8999 e toll free U S 800 272 2775 fax 508 429 5732 e www harvardapparatus com 11
13. en 1 to 99 seconds Pipette tip diameter and hence delivered volume are easy determinations once the timing and pressure are known Versatile Deliver volumes from femtoliters to microliters with the same instrument resulting in a wide range of applications Pressure Capabilities The PLI 100 features two negative and three positive pressure pneumatic capabilities The negative or vacuum functions allow the user to 1 Fill micropipettes from their tips reducing wastage of valuable injectables 2 Provide a means to secure and manipulate a cell using a holding pipette 4 Previous Next Section Table of Contents Main LEL Coi Contents Contact Us PLI 100 Medical Systems Pico Injector continued The positive pressures allow the user to 1 Eject precise amounts of fluids 2 Create a balance pressure which prevents backflow into the micropipette following an injection 3 Clear a micropipette of material in it Unique Features The PLI 100 s important Balance Hold and Clear functions are not found on other microinjection units Balance In addition to the ejection pressure the Pico Injector offers a secondary balance pressure This secondary balance maintains a positive pressure on the injection pipette before and after injections This eliminates dilution caused by capillary action and aids in the prevention of clogging Wasteful continuous injection which often occurs
14. ined by turning the metal colored actuator screw control on the top of the syringe The syringe provides a solution to the problem of capillary action when working with small micropipettes A small balance equilibrating pressure can be maintained to offset the effects of capillary action A conveniently situated release button on top of the screw control is provided to achieve equilibration Order Product EC1 69 0105 Screw Actuated Air Syringe e Ideal for oil filled injection aspiration e Easy to use e Hamilton syringe incorporated e Accurate e Sensitive control e Low dead air volume e High suction pressure e Easy to exchange or replace the syringe Screw Actuated Micrometer Driven Hamilton Syringe This screw actuated syringe is a micrometer actuated syringe mechanism mounted on a small base This syringe is for oil air assisted micro Injections and micro aspirations Its compact dimensions enable it to be ergonomically positioned next to the control lever of a micromanipulator to facilitate single handed operation of both instruments suction injection is achieved by using the rotary actuator with a fine micrometer thread which has a 15 mm movement range A 1 ml gastight Hamilton glass syringe with a Luer taper is incorporated A Luer taper hypodermic needle is mounted on the taper of the Hamilton syringe The syringe is connected by hard polyethylene tubing to a micropipette which in turn may be fitted to a micromanipul
15. l Bushing Replacement for use with PLI PH1 and PLI PH1A Pipette Holders Stainless Steel Pipette Cap Replacement for use with PLI PH1 and PLI PH1A Pipette Holders Stainless Steel Hose Nut Replacement for use with PLI PH1 and PLI PH1A Pipette Holders Pipette Holder Hardware Replacement Kit for use with PLI PH1 and PLI PH1A Pipette Holders Includes 1 each PLI SSB PLI PC PLI HN and PLI SRG Stainless Steel Pipette Holder PLI PH series Adapter for Eppendorf ECET FEMTOTIP Optional Easy Insert Acrylic Pipette Holder p 101 6 mm 4in ______ Tue Capillary Glass OD Order EC1 64 1626 EC1 64 1627 EC1 64 1628 EC1 64 1629 Model A016 1 0 A016 1 2 PLI PPH A016 1 2 Barrel Diameter 8 mm 0 312 in TEE Dig Tubing Diameter 2 mm 0 08 in Product Acrylic Pipette Holder for 1 0 mm Pipettes Acrylic Pipette Holder for 1 2 mm Pipettes Acrylic Pipette Holder for 1 5 mm Pipettes Acrylic Pipette Holder for 2 mm Pipettes 1 Previous Next Section Table of Contents Main LEL CK Contents Contact Us PM 3 and PM 4 8 and 4 Channel Pressure Injection Systems EC1 69 0153 8 Channel Pressure Injection System e Multiple function system for micro injection and perfusion e Up to 8 injection perfusion outputs one hold cell output and one synchronized drain out channel e Programmable timers counters and step sequences which can be stored for
16. of the above experiments and get meaningful results the tools equipment used in these experiments must provide not only the specific functionality but they also need to be of the highest quality and provide the necessary reliability accuracy and repeatability to insure proper results Harvard Apparatus Inc manufactures and sells the complete range of product that are needed to successfully microinject and or microperfuse Pipette Pullers Micromanipulators Harvard Apparatus phone 508 893 8999 e toll free U S 800 272 2775 fax 508 429 5732 www harvardapparatus com 2 PLS 1 Pico Injection Micromanipulator System Previous Section Table of Contents Main LEL CK Contents Contact Us The PLS 1 Pico Injection Micromanipulator System combines our popular PLI 100 pico injector a motorized micromanipulator and magnetic base in a single package The PLI 100 Pico Injector delivers a wide range of volumes through micropipettes by applying a regulated pressure for a set period of time The system features 5 pressures inject balance clear fill and hold The injector comes complete with input and output hoses foot switch and pipette holder For precise and convenient movement of the electrode our three axes motorized micromanipulator and push button controller are included This micromanipulator features hands free spatial resolution of 0 5 um which enables positioning in the sub micro range Flexible motor c
17. oupling ensures no vibration of probe during movement The MB B magnetic base provides a convenient position device for the micromanipulator Order Model Product EC1 64 1608 PLS 1 Pico Injector Micromanipulator System EC1 65 0002 PLI 100 Plus Pico Injector EC1 60 0571 Right handed motorized micromanipulator EC1 60 0577 Push button controller EC1 64 0060 MB B Magnetic base Harvard Apparatus phone 508 893 8999 e toll free U S 800 272 2775 fax 508 429 5732 www harvardapparatus com 3 Previous Next Section Table of Contents Main LEL Coi Contents Contact Us PLI 100 Medical Systems Pico Injector MIKO THOET e MMEasuRK Ting OR At eon poas fa W Dining af E liste 1 GD Gd 6 THES eae aq GC e mate OUT kons Caio Titi 5 pressures inject balance clear fill and hold Reliable e Femtoliter to microliter injections e Reproducible performance e Easy to use e Popular applications Injection of mouse frog zebrafish and other oocytes Extracellular brain injections Injection of DNA mRNA microbeads neurotransmitters kinases and other proteins Most Published Injector The PLI 100 Pico Injector reliably delivers a wide range of volumes through micropipettes by applying a regulated pressure for a digitally set period of time Compressed gas allows the user to deliver desired volumes from femtoliters to microliters while simultaneously holding a
18. rm injections into other specimens applications that previously could not be performed Positive displacement technology and the use of precision micropipettes eliminate the need for tedious calibration when the viscosity of the sample changes Contaminants in the sample do not hinder or change the injection volume as with some other injectors An optional universal mounting adapter provides easy mounting of the Nanoject II to a variety of micromanipulators and stereotaxic frames Needs Micromanipulator to attach to Support Stand Specifications Operation Automatic Softening point 780 C 1 14 x 0 053 mm 0 045 x 0 021 in OD x ID Fast 46 nl sec Slow 23 nl sec Glass Properties Glass Dimensions Injection Speed Fill Speed Fast 46 nl sec Slow 23 nl sec Empty Speed 92 nl sec Volume Range 2 3 nl to 69 0 nl Change Volume Dip switches 1 to 4 Change Inject Speed Dip switch 5 Weight 99 2 g 3 5 oz Order Product EC1 69 0130 Nanoject II Nanoliter Injector 115 V 60 Hz US Plug EC1 69 0131 Nanoject II Nanoliter Injector 230 V 50 Hz European Plug EC1 69 0132 Nanoject II Nanoliter Injector 230 V 50 Hz UK Plug Replacement Parts EC1 69 0133 Replacement Glass 88 9 x 1 14 x 0 053 mm 3 5 x 0 045 x 0 021 in L x OD x ID Vial of 100 EC1 69 0134 Replacement Glass 177 8 x 1 14 x 0 053 mm 7 x 0 045 x 0 021 in Lx OD x ID Vial of 100 EC1 69 0135 Flared Glass 90 mm 3 5 in Vial of 100 EC
19. s pressure readings can also be recovered on the display for setting comparison Besides programmable timers and counters there are 16 programmable and savable injection perfusion sequences with 32 programmable steps in each sequence Each sequence can be repeated continuously or triggered manually The interval time between steps can be programmed for automatic sequential cycle or manual trigger There are two foot switch connectors for sequential step trigger and injection trigger A digital remote port can be connected to an optional remote key pad or be controlled by a computer With exceptional versatility and extremely precise control either the PM 4 or 8 is an ideal multi channel injection perfusion engine Connection Diagram for Extracellular Micro Perfusion and Intracellular Micro Injection Systems PM 4 8 Programmable Pressure Injector 30 to 100 psi Pressure Source PM4 4 Channe Injection PM8 8 Channel Injection Pressure Output 4 Barrel Pipette perenne O z PP 8 f 7 Barrel Pipette Drain Out Liquid N Drain Hold Cell Pipette Pipette Single Barrel Injection Pipette Tested Cell Extracellular Micro Perfusion Connection FSW Foot Switch PM KP Remote Injection Time Signals Key Pad of 4 or 8 Channels Hold Cell Pipette onde Balle Injection Pipette Intracellular Micro Injection Connection Harvard Apparatus phone 508 893 8999 e toll free U S 800 272 2775 fax 508 429 573
20. t do not need suction Pico Injector Basic Model and Acces sories Shown Above Plus One Each PLI FS Foot Switch PLI PH1 Pipette Holder and PLI IHA Input Hose Adapter 6 Previous Next Section Table of Contents Main LEL CK Contents Contact Us PLI 100 and PLI 90 PLI 100 and PLI 90 Accessories Order Accessories EC1 65 0029 EC1 65 0030 Hosing EC1 65 0006 EC1 65 0007 EC1 65 0008 EC1 65 0009 EC1 65 0010 EC1 65 0011 EC1 65 0012 Harvard Apparatus phone 508 893 8999 e toll free U S 800 272 2775 fax 508 429 5732 www harvardapparatus com Model PLI FS PLI RM PLI IHA PLI IHO PLI IHN PLI OHO PLI OHN PLI HHO PLI HHN Product Footswitch for Inject Hold Fill or Gate Order more than one if frequent use of footswitch is needed for more than one of these functions Frame to Mount PLI 90 or PLI 100 in 19 in Wide Instrument Rack Input Hose Adapter in Male EC1T Pipe Thread Gas Bottle Regulator Fitting Connects with PLI IHO N Gas Input Hose Tygon Tubing 9 ft x 0 250 in 6 35 mm x 0 125 in 3 18 mm x 0 062 in 1 57 mm Lx OD x ID x Wall Thickness Connects to PLI IHA and PLI 90 100 Gas Input Brass Screw Fitting Type Old Gas Input Hose Tygon Tubing 9 ft L x 0 250 in 6 35 mm OD x 0 125 in 3 18 mm ID x 0 062 in 1 57 mm Wall Thickness Connects to PLI IHA and PLI 90 100 Gas Input Quick Connect Disconnect Type New O
21. ulated multi turn control other ranges available upon request Balance Pressure Internally produced 12 0 p s i 82 kPa unregulated Internally produced 0 to 3 in H 0 0 to 0 75 kPa or 0 to 0 1 p s i regulated Fill Vacuum Holding Vacuum Clearing Pressure Input gas pressure unregulated Injection Timer Pulse Width Injection Count Display 0 01 to 0 99 sec in 10 msec steps 1 to 99 sec in 1 sec steps Digital 0 through 9999 Duration Mode Internally timed or externally gated Time Trigger Front panel foot switch or external TTL pulse BNC Pressure Units p s i kPa switch selectable Pressure Monitor BNC connector 10 mV p s i Pressure Readout Inject balance clear output port 100 110 220 240 VAC 220 W 0 1 full scale Inject fill hold and gated provided in plus and deluxe pkgs 6 8 kg 15 Ib 11 x 38 x 25 5 cm 5 x 15 x 10 in Input output and holding hoses Line Voltage Power Usage Meter Accuracy Foot Switches Weight Dimensions Hx Wx D Accessories Supplied Order Model EC1 65 0001 PLI 100 Product PLI 100 Basic Pico Injector with Injection Balance Clear Filling and Holding Pressures Comes with Input Hose Output Hose Holding Hose Power Cord and Instruction Manual PLI 100 Plus Pico Injector with Injection Balance Clear Filling and Holding Pressures Comes with Input Hose Output Hose Holding Hose Power Cord Instruction Manual Foot Switch 65 0029 Pipette
22. utput Hose Tygon Tubing 9 ft L x 0 071 in 1 80 mm OD x 0 039 in 1 mm ID x 0 016 in 0 41 mm Wall Thickness Connects to MSC Pipette Holders Brass Screw Fitting Type Old Output Hose Tygon Tubing 9 ft L x 0 071 in 1 80 mm OD x 0 039 in 1 mm ID x 0 016 in 0 41 mm Wall Thickness Connects to MSC Pipette Holders and PLI 90 100 Gas Output Quick Connect Disconnect Type New Holding Hose with Cell Release Bulb 9 ft L x 0 071 in 1 80 mm OD x 0 039 in 1 mm ID x 0 016 in 0 41 mm Wall Thickness Connects to MSC Pipette Holders and PLI 100 Hold Output Brass Screw Fitting Type Old Holding Hose with Cell Release Bulb 9 ft L x 0 071 in 1 80 mm OD x 0 039 in 1 mm ID x 0 016 in 0 41 mm Wall Thickness Connects to MSC Pipette Holders and PLI 100 Hold Output Quick Connect Disconnect Type New Order Model Product Pipette Holder Adapter Parts EC1 65 0013 EC1 65 0014 EC1 65 0017 EC1 65 0015 EC1 65 0018 EC1 65 0019 EC1 65 0020 EC1 65 0021 EC1 65 0022 PLI PH1 PLI PH1A PLI SRG PLI SRG1 5 PLI SSB PLI PC PLI HN PLI PH KIT PLI PHA Stainless Steel Pipette Holder 130 mm L for 1 to 1 5 mm OD Glass Pipettes Stainless Steel Pipette Holder 80 mm L for 1 to 1 5mm OD Glass Pipettes Silicone Rubber Gasket Replacement for use with PLI PH1 and PLI PH1A 10 2 cm 12 in Silicone Rubber Gasket Replacement for use with 1 5 mm Glass Pipettes Stainless Stee
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