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Biotage® Analytical Sample Preparation

1. Bioanalytical Applications and Products for Bioanalysis ISOLUTE Ion Exchange Sorbents e ISOLUTE cation exchange sorbents for extraction of basic drugs e ISOLUTE anion exchange sorbents for extraction of acidic drugs For very polar ionizable compounds that cannot be retained using a non polar retention mechanism ISOLUTE ion exchange sorbents offer an alternative that can provide selective extraction with very clean extracts See pages 52 59 164 167 for more details and ordering information The QuickStart Guide at the back of this catalog provides methodology details for these sorbents Chemistry Data Sheets are also available see relevant sorbent ordering pages Formats for High Throughout Sample Preparation 96 well plates from Biotage are available in two formats fixed well plates for routine high throughput applications and the modular Array format for routine processing of fewer than 96 samples and method development Both formats are processed on the VacMaster 96 Sample Processing Manifold and are compatible with commonly used liquid handling systems See page 11 13 for more details Standard 1 and 3 mL SPE columns can be processed on liquid handling systems for automated sample preparation Some systems including the Gilson SPE 215 require tab less columns held in a rack system that allows a microplate footprint Tabless columns are available from Biotage at the same price as the tabbed equivalent C
2. C8 C8 EC Octyl 96 well SPE plates 35 36 156 157 Bulk sorbent 66 SPE columns 35 36 156 157 C18 C18 EC n Octadecyl 96 well SPE plates 32 33 156 157 Bulk sorbent 66 SPE columns 32 33 156 157 Caps SPE column 87 141 Carboxypropyl acid see CBA CBA Carboxypropyl acid 96 well SPE plates 56 166 167 Bulk sorbent 66 SPE columns 56 166 167 CH EC Cyclohexyl 96 well SPE plates 41 158 159 SPE columns 41 158 159 CN CN EC Cyanopropyl 96 vvell SPE plates 44 45 158 159 SPE columns 44 45 158 159 Collection plates 96 vvell 91 Custom manufacturing service 187 189 Cyanopropyl see CN CN EC Cyclohexyl see CH EC D Depth filter reservoirs 86 Depth filter SPE columns with integral depth filter 140 DIOL 2 3 Dihydroxypropoxypropyl 96 well SPE plates 61 168 169 SPE columns 61 168 169 Drying adapter VacMaster 101 Empty reservoirs 87 ENV Hydroxylated polystyrene divinylbenzene 96 well SPE plates 30 121 156 157 SPE columns 30 121 136 149 156 157 Environmental analysis 136 143 EPH SPE columns 138 Ethyl see C2 C2 EC Ethylbenzene sulfonic acid see SCX 3 Evaporation 112 115 EVOLUTE 22 27 96 120 126 136 146 Extraction caps 141 F Filtration columns 88 149 Filtration plates 90 Florisil SPE columns 62 170 171 Food analysis 146 151 see Agrochemical Food section Forensic analysis 126 133 Frit inserters 148 Frits polyethylene 88 Frits sta
3. 89 905 0025 1 47 602 0010 01 25 712 0010 62 820 0140 76 77 905 0025 R 47 602 0025 P01 25 27 712 0020 A 62 130 131 132 905 0100 P01 47 132 712 0020 B 62 820 0140 96 905 0100 47 602 0025 R 25 712 0020 H 62 820 0200 P01 76 77 906 0002 A 48 603 0002 A 26 712 0050 B 62 130 131 132 133 906 0005 A 48 603 0002 AG 26 96 712 0050 C 62 820 0200 T 76 906 0010 A 48 603 0010 P01 25 712 0050 H 62 820 0290 D 76 906 0010 G 48 603 0025 P01 25 27 712 0050 L 62 820 0400 01 76 906 0013 H 48 603 0025 R 25 712 0100 B 62 820 0400 T 76 906 0025 PO1 48 604 0002 A 26 712 0100 C 62 820 0690 76 906 0025 R 48 604 0010 P01 25 712 0200 D 62 820 1420 F 76 906 0100 P01 48 604 0025 P01 25 27 712 0500 E 62 902 0002 A 46 128 906 0100 48 142 712 2000 62 902 0002 AG 46 96 915 0001 30 604 0025 25 713 0010 63 902 0005 46 128 915 0002 30 610 0005 26 713 0020 63 902 0010 A 46 915 0002 30 610 0005 26 96 713 0020 B 63 902 0010 B 46 915 0005 A 30 610 0010 B 26 713 0050 B 63 902 0013 A 46 109 915 0005 AG 30 96 610 0010 H 26 27 713 0050 C 63 128 130 131 133 915 0005 B 30 132 713 0100 B 63 902 0013 BG 46 96 915 0005 30 610 0020 B 26 713 0100 C 63 902 0013 C 46 128 915 0010 30 610 0020 26 27 713 0200 D 63 902 0013 CG 46 96 915 0010 30 142 713 0500 63 Part Number Page 986 0025 R 49 M06 0002 G 19 127 SD 9600 DHS JP 112 915 0010 BG 30 96 986 0100 P01 49 132 SD 9600 DHS NA 112 915 00
4. Extraction from aqueous matrix using non polar interactions for acidic neutral and basic TN101 compounds This sorbent exhibits a different selectivity compared with C18 and C8 phases when both aromatic and non aromatic compounds are being extracted Extraction from aqueous matrix using both non polar interactions for acidic neutral and TN101 8 112 basic compounds and secondary silanol or ionic interactions for basic compounds only Extraction from aqueous matrix using non polar interactions for acidic neutral and basic TN101 compounds For more information on non polar SPE procedures see the QuickStart Guide page 173 185 Extraction from aqueous matrix using both non polar interactions for acidic neutral and TN101 112 42 basic compounds and secondary silanol or ionic interactions for basic compounds only This sorbent exhibits a different selectivity compared with C18 and C8 phases when both aromatic and non aromatic compounds are being extracted For in depth technical information on the use of non polar sorbents refer to the Chemistry Data Sheets listed in the table above 159 160 Mixed Mode Sorbents Isolate acidic and basic analytes from biological fluids and other complex matrices with Biotage s range of mixed mode sorbents The sorbents both silica and polymer based provide two primary retention mechanisms non polar hydrophobic and ion exchange anion or cation The dual retention m
5. Methanol formic acid 98 2 v v Methanol ammonium hydroxide 95 5 v v Methanol acetic acid 98 2 v v Methanol ammonium hydroxide 95 5 v v 0 2M monochloroacetic acid in methanol Methanol Evaluate other solvents e g hexane acetone ethyl acetate etc if additional selectivity is required See previous section for additional information for elution of basic compounds using non polar SPE 181 182 Conditioning Equilibration Sample pH Polar silica based sorbents see page 44 52 53 60 61 168 171 ISOLUTE SI NH2 CN DIOL PSA Non polar solvent e g hexane preferably the same solvent as the sample N A N A Ion exchange silica based sorbents see page 52 59 164 167 SCX 2 Strong cation exchange SCX 3 Strong cation exchange with significant non polar secondary interactions CBA Weak cation exchange sorbent pK 4 8 PE AX Strong anion exchange acetate counter ion SAX Strong anion exchange chloride counter ion NH2 Weak anion exchange sorbent pK 9 8 Methanol or Acetonitrile Methanol or Acetonitrile Methanol or Acetonitrile Methanol or Acetonitrile Methanol or Acetonitrile Methanol or Acetonitrile 20 50 mM buffer the same pH as the sample 20 50 mM buffer the same pH as the sample 20 50 mM buffer the same pH as the sample 20 50 mM buffer the same pH as the sample Acetate
6. Reproducible sorbent mass packed into SPE columns Column capacity and analyte elution volume requirements are constant e Consistent column to column and batch to batch recoveries Well controlled particle size distribution with minimal fines from column to column and batch to batch Reliable sample processing using manifolds and automated devices e Consistent flow through columns from column to column and batch to batch Low back pressure automation friendly e Gravity loading of samples with some column configurations e No fines in final extract to plug injectors or absorb analytes when sample is reconstituted in another solvent No channeling in sorbent beds minimizes sorbent mass requirements reducing elution volumes and costs e Minimal drying time reproducible from batch to batch e Large sorbent mass columns with good flow characteristics Quality Assurance Report Biotage Part ha 221 0010 Description ISGLUTE CIB LEE 100mg Im SPE Columnas Lot Mo 102210026 Sorbent Batch 221 0 02 roda hai See mi eee ima dr Deren ra Hp ceed LE MG 00 ee er ril Bol Fun hubiteliri l Pe eng LAA hase Material Analysis Material HPLC Araiysis en q mar Ai B Eu Pris 1 AA AO buree ras Less d Sule MA ae Elemental Analysis Carter Loading WE TE Bat Pers Cover lir 1 6 Fear Ta hiy p Hand Pope Ha
7. EVOLUTE Array WAX 25 mg imL Wells EVOLUTE Quantity 100 100 100 100 100 100 100 25 26 EVOLUTE Advanced Polymeric Sorbents for LC MS MSApplications Part number SPE Columns EVOLUTE ABN 600 0001 A 600 0002 A 600 0002 AG 600 0002 B 600 0002 H 610 0005 B 610 0005 BG 610 0010 B 610 0010 H 610 0020 B 610 0020 C 610 0050 C EVOLUTE CX 601 0001 A 601 0001 AG 601 0002 A 601 0002 AG 611 0005 B 611 0005 BG 611 0010 B 611 0010 BG 611 0010 H 611 0020 B 611 0020 C 611 0050 C 611 0050 CG EVOLUTE VVCX 602 0002 A 612 0005 B 612 0010 B 612 0010 H 612 0020 C 612 0050 C EVOLUTE AX 603 0002 A 603 0002 AG 613 0005 B 613 0010 B 613 0010 BG 613 0010 H 613 0020 C 613 0050 C 613 0050 CG EVOLUTE VVAX 604 0002 A 614 0005 B 614 0010 B 614 0010 H 614 0020 C 614 0050 C Tabless columns for use with Pressure Positive Pressure Manifolds and other automated SPE systems Description EVOLUTE ABN 10 mg 1 mL EVOLUTE ABN 25 mg 1 mL EVOLUTE ABN 25 mg 1 mL Tabless EVOLUTE ABN 25 mg 3 mL EVOLUTE ABN 25 mg 10 mL XL EVOLUTE ABN 50 mg 3 mL EVOLUTE ABN 50 mg 3 mL Tabless EVOLUTE ABN 100 mg 3 mL EVOLUTE ABN 100 mg 10 mL XL EVOLUTE ABN 200 mg 3 mL EVOLUTE ABN 200 mg 6 mL EVOLUTE ABN 500 mg 6 mL EVOLUTE CX 10 mg 1 mL EVOLUTE CX 10 mg 1 mL Tabless EVOLUTE CX 25 mg 1 mL EVOLUTE CX 25 mg 1 mL Tabless EVOLUTE CX 50 mg 3 mL EVOLUTE CX 50 mg 3 mL Tabless EVOLUTE CX 100 mg 3 mL
8. Nominal Application Chemistry Ordering Moisture Data Information Content Sheets Page Extraction of polar compounds from a non polar sample matrix Alternative to silica based polar sorbents Minimal retention of basic compounds Activated for separation of chlorinated pesticides Extraction of polar compounds from a non polar sample matrix Alternative to silica based polar sorbents Extraction of polar compounds from a non polar sample matrix Particularly retentive for electronegative analytes Alternative to silica based polar sorbents Extraction of polar compounds from a non polar sample matrix Has negative surface for specific retention of cations Alternative to silica based polar sorbents For more information on polar SPE procedures see the QuickStart Guide page 174 184 QuickStart Guide QuickStart Guide to SPE Introduction Sample preparation prior to analysis is as important to the success of an assay as the analytical technique itself This is especially true when low levels of detection are required or for analytes in complex matrices such as biological fluids tissue foodstuffs agricultural products and environmental samples Solid Phase Extraction SPE is a powerful technique for rapid selective sample preparation The versatility of SPE allows it to be used for a variety of purposes such as e Purification e Trace enrichment e Solvent exchange analytes are transferred from one matr
9. 10 columns 1 C50000 RapidTrace x 6 ul 5 columns 1 125713 3 mL and 1 mL only with appropriate plunger see accessories RapidTrace Start Up Kit with Software 1 C52006 RapidTrace Notebook Controller 1 C52689 Racks Each RapidTrace requires one rack 13 x 100 mm Sample Tube 1 74 12 x 75 Fraction Tube an 13 x 100 mm Sample Tube Pk 7 12 x 75 Fraction Tube 13 x 100 mm Sample Tube 13 x 100 mm Fraction Tube 16 x 100 mm Sample Tube 16 x 100 mm Fraction Tube 1 13 x 100 mm Sample Tube 1 C56536 12 x 75 mm Fraction Tube 9 Position Chilled Rack 40 mL sample flask 5 position 1 0133068 16 x 100 Fraction Tube 10 position 108 Applications Application number AN727 AN725 AN744 AN728 AN730 AN748 AN749 AN729 AN704 AN726 AN724 Visit the Sample Preparation Application Database at www biotage com applications Analyte Basic Drugs of Abuse Benzoylecgonine Catecholamines Cyclosporine Environmental contaminants including Dioxins and Furans Ethyl Glucuronide Melamine Morphine and Codeine Extractable petroleum hydrocarbons Tricyclic Antidepressants Vitamin D Matrix Biological fluids Urine Plasma Whole blood Soil and sediments Urine Milk Urine Soil Whole blood Plasma Format RapidTrace and ISOLUTE HCX 200 mg 3 mL column RapidTrace and ISOLUTE HCX 130 mg 3 mL column RapidTrace and EVOLUTE WCX
10. 100 mg 1 mL 100 351 0010 B ISOLUTE CH EC 100 mg 3 mL 50 351 0020 B ISOLUTE CH EC 200 mg 3 mL 50 351 0050 B ISOLUTE CH EC 500 mg 3 mL 50 351 0050 C ISOLUTE CH EC 500 mg 6 mL 30 351 0050 H ISOLUTE CH EC 500 mg 10 mL 50 351 0100 C ISOLUTE CH EC 1 g 6 mL 30 ISOLUTE Array CH EC Loose Wells Part number Description Quantity 351 0025 R ISOLUTE Array CH EC 25 mg 1 mL wells 100 Pre assembled ISOLUTE Array plates are available To order add the suffix P to the equivalent loose well part number Support Documents TN101 Method Development in Solid Phase Extraction using Non polar ISOLUTE SPE Columns for the Extraction of Aqueous Samples See pages 158 and 159 for additional technical information about ISOLUTE CH EC r An Applications Matrix Analytes Retention Mechanisms Primary Non polar Aqueous Wide polarity range Secondary Polar and weak cation exchange Chemical structure of phenyl silane covalently bonded to the surface of a silica particle Ordering Information ISOLUTE PH SPE Columns Part number Description Quantity 360 0002 A ISOLUTE PH 25 mg 1 mL 100 360 0005 A ISOLUTE PH 50 mg 1 mL 100 360 0010 A ISOLUTE PH 100 mg 1 mL 100 360 0010 B ISOLUTE PH 100 mg 3 mL 50 360 0050 B ISOLUTE PH 500 mg 3 mL 50 360 0050 C ISOLUTE PH 500 mg 6 mL 30 360 0100 C ISOLUTE PH 1 g 6 mL 30 ISOLUTE 96 PH Fixed Well Plates Part number Description Quantity 360 0025 P01 ISOLUTE 96 PH 25 mg plate 1 360 0050 P01 ISOLUTE 96 PH
11. EN Waxed Fruit and Vegetable Clean up Tube 25 Q0060 15V AOAC Pigmented Fruit and Vegetable Clean up Tube 25 Q0070 15V EN Pigmented Fruit and Vegetable Clean Up Tube 25 Q0080 15V More information for ISOLUTE QuECHhERS be found on pages 69 71 ISOLUTE Products for Matrix Solid Phase Dispersion MSPD Matrix Solid Phase Dispersion MSPD is a technique designed for extrac tion of solid or semi solid samples such as meat or vegetable material The sample and MSPD sorbent are ground together in a pestle and mortar forming a blend which is added to a fritted extraction column A top frit is inserted and a series of solvents are used to sequentially elute interference compounds and the analytes of interest ISOLUTE MSPD sorbents have been optimized to rapidly produce a homogenous reproducible sorbent sample blend compared with traditional SPE or preparative grade sorbents Ordering Information ISOLUTE MSPD sorbents Description Quantity Part number ISOLUTE MSPD Grade C18 100 g 9370 0100 ISOLUTE MSPD Grade C18 EC 100 g 9371 0100 ISOLUTE MSPD accessories Description Quantity Part number Frit inserter 6 mL C columns 5 120 1151 C Frit inserter 15 mL D columns 5 120 1151 D For ordering information for empty columns and frits see pages 87 88 For more information request Chemistry Data Sheet TN111 Agrochemical and Food Applications and Products for Agrochemical and Food Analysis ISOLUTE Multimode SPE
12. FFEFEFFE bey an mi Fupu ina NH isi Pan FT baxar p te Gi ra EVOLUTE Sample Preparation Products EVOLUTE Advanced Polymeric Sorbents for LC MS MSApplications EVOLUTE Sample Preparation Products EVOLUTE polymer based SPE products offer a simple robust generic approach to the isolation and clean up of small molecules from a variety of matrices including plasma serum whole blood urine foodstuffs and water e Extract a wide range of analytes using a choice of 5 sorbent chemistries e Simple fast method development e Robust water wettable sorbents are stable from pH 0 14 e Formats to match all sample volume and throughput demands e Methodology optimized for clean extracts in LC MS e Excellent protein and phospholipid removal properties EVOLUTE sorbent chemistry The EVOLUTE family offers a full range of SPE options with sorbents and methodologies optimized to provide solutions to a wide variety of sample preparation problems The five sorbent chemistries are based on a modified polystyrene divinylbenzene polymer incorporating hydroxy functionalized oligomers EVOLUTE ABN Figure 1 The mixed mode sorbents are manufactured by surface modification of EVOLUTE ABN The water wettable hydrophobic hydrophilic balanced sorbents maintain their optimum retention elution capabilities even if they dry completely during processing Polar hydrophilic groups Figure 1 EVOLUTE AB
13. JUrboVap Vaporators ana concentrators TurboVap Product Family Biotage offers a range of TurboVap Concentration Workstations for sample evaporation This section is designed to help you select the most suitable TurboVap for your research requirements as well as answer some of the more common questions regarding services maintenance and applications Biotage TurboVap units use a patented vortex evaporation system that increases the speed of evaporation by a factor of 10 compared with other techniques The combination of gas flow and temperature is used to optimize sample drying Nitrogen is recommended due to its inert nature which minimizes sample oxidation Alternatively clean oil free compressed air at 60 psi can be used if the sample is stable and nitrogen is not available As gas usage can be high if large numbers of samples are prepared a nitrogen generator or bulk liquid nitrogen supply is recommended to minimize cylinder handling TurboVap LV Volume Range 1 mL to 60 mL The TurboVap LV is ideal for sample volumes of 1 mL to 30 mL that need to be evaporated in GC vials microcentrifuge tubes conical bottom tubes or test tubes This instrument offers a high throughput of 50 sample positions using the standard range of racks available Evaporation is based on a water bath to give an even temperature and a gas flow delivered over a set time period This combination gives the option to evaporate samples to dryness if required
14. gt Competitor O a idil u Figure 2 Pore size distribution of EVOLUTE ABN compared vvith a competitor product Note the tight distribution of pore size in the 15 40 um range relative to the broad removal using the EVOLUTE ABN generic method distribution of the competitor product Figure 3 Gel electrophoresis image shovving protein 23 24 EVOLUTE Advanced Polymeric Sorbents for LC MS MSApplications Method development using EVOLUTE SPE sorbents EVOLUTE SPE products and methods can be used to extract many compound classes either singly or as multiple analytes from aqueous samples using either non polar hydrophobic or mixed mode SPE Analyte Functionality EVOLUTE sorbent selection CX WCX AX Neutral Weakly acidic pK 2 8 Strongly acidic pK lt 2 Weakly basic pK 2 10 Strongly basic pK gt 10 Amphoteric Acidic neutral basic mix Fractionation acidic neutral basic Table 2 EVOLUTE sorbent selection First choice Alternative Method development using EVOLUTE SPE products is fast and simple Generic procedures for each sorbent and a wealth of optimization tips are described in the comprehensive EVOLUTE User Guide Download the EVOLUTE User Guide from biotage com EVOLUTE applications The EVOLUTE SPE family has been successfully used for extraction of a wide range of analytes from many matrices including plasma urine whole blood water milk and tissue extracts We are con
15. of Non aqueous Samples TN104 Method Development in Solid Phase Extraction using ISOLUTE NH2 SPE Columns for the Extraction of Aqueous Samples See pages 164 and 165 for additional technical information about ISOLUTE NH2 Applications Matrix 0 Analytes Retention Mechanisms S 0 8j CH NH CH NH Primary Weak anion exchange SI OH Chemical structure of PSA silane covalently bonded to the surface of a silica particle Ordering Information ISOLUTE PSA SPE Columns Part number Description Quantity 480 0010 A ISOLUTE PSA 100 mg 1 mL 100 480 0010 B ISOLUTE PSA 100 mg 3 mL 50 480 0020 B ISOLUTE PSA 200 mg 3 mL 50 480 0050 B ISOLUTE PSA 500 mg 3 mL 50 480 0050 C ISOLUTE PSA 500 mg 6 mL 30 480 0100 C ISOLUTE PSA 1 g 6 mL 30 ISOLUTE 96 PSA Fixed Well Plates Part number Description Quantity 480 0025 P01 ISOLUTE 96 PSA 25 mg plate 1 480 0100 P01 ISOLUTE 96 PSA 100 mg plate 1 ISOLUTE Array PSA Loose Wells Part number Description Quantity 480 0025 R ISOLUTE Array PSA 25 mg 1 mL wells 100 480 0100 T ISOLUTE Array PSA 100 mg 2 mL wells 100 Pre assembled ISOLUTE Array plates are available To order add the suffix P to the equivalent loose well part number Support Documents TN102 Method Development in Solid Phase Extraction using Polar ISOLUTE SPE Columns for the Extraction of Non aqueous Samples TN105 Method Development in Solid Phase Extraction using ISOLUTE PSA SPE Columns for the Extraction of Aque
16. 100 mg 1 mL 100 321 0010 B ISOLUTE C2 EC 100 mg 3 mL 50 321 0010 G ISOLUTE C2 EC 100 mg 10 mL 50 321 0020 B ISOLUTE C2 EC 200 mg 3 mL 50 321 0050 B ISOLUTE C2 EC 500 mg 3 mL 50 321 0050 C ISOLUTE C2 EC 500 mg 6 mL 30 321 0050 H ISOLUTE C2 EC 500 mg 10 mL 50 321 0100 C ISOLUTE C2 EC 1 g 6 mL 30 ISOLUTE 96 C2 EC Fixed Well Plates Part number Description Quantity 321 0025 P01 ISOLUTE 96 C2 EC 25 mg plate 1 ISOLUTE Array C2 EC Loose Wells Part number Description Quantity 321 0025 R ISOLUTE Array C2 EC 25 mg 1 mL wells 100 321 0050 R ISOLUTE Array C2 EC 50 mg 1 mL wells 100 321 0100 T ISOLUTE Array C2 EC 100 mg 2 mL wells 100 Pre assembled ISOLUTE Array plates are available To order add the suffix P to the equivalent loose well part number Support Documents TN101 Method Development in Solid Phase Extraction using Non polar ISOLUTE SPE Columns for the Extraction of Aqueous Samples See pages 158 and 159 for additional technical information about ISOLUTE C2 EC 3 Si o si 7 O Applications Matrix Analytes Retention Mechanism Aqueous Wide polarity range Primary Non polar Si 0 Si CH Endcapped sorbent to minimize secondary silanol interactions Chemical structure of cyclohexyl silane and trimethyl silyl group covalently bonded to the surface of a silica particle Ordering Information ISOLUTE CH EC SPE Columns Part number Description Quantity 351 0010 A ISOLUTE CH EC
17. 39 120 1151 D 148 220 0025 P01 32 240 0050 B 34 321 0005 A 40 120 1200 83 91 220 0025 R 32 240 0050 C 34 321 0010 A 40 120 1201 83 91 220 0050 B 32 240 0050 H 34 321 0010 40 120 1202 83 91 220 0050 BG 32 96 240 0100 34 321 0010 G 40 120 2040 P01 83 220 0050 C 32 240 0200 D 34 321 0020 40 120 2040 R 83 220 0050 CD 140 240 0500 E 34 321 0025 01 40 120 2040 83 220 0050 32 290 0002 35 321 0025 40 121 0001 98 103 220 0050 P01 32 290 0005 35 321 0050 40 121 0002 98 103 220 0050 32 290 0010 35 131 321 0050 40 Part Number 321 0050 H 321 0050 R 321 0100 C 321 0100 T 351 0010 A 351 0010 B 351 0020 B 351 0025 R 351 0050 B 351 0050 C 351 0050 H 351 0100 C 360 0002 A 360 0005 A 360 0010 A 360 0010 B 360 0025 P01 360 0025 R 360 0050 B 360 0050 C 360 0050 P01 360 0050 R 360 0100 C 360 0100 P01 360 0100 T 361 0005 A 361 0010 A 361 0010 B 361 0020 B 361 0025 P01 361 0025 R 361 0050 B 361 0050 C 361 0050 R 361 0100 C 380 0010 A 380 0010 B 380 0020 B 380 0025 P01 380 0025 R 380 0050 B 380 0050 C 380 0100 C 390 0010 A 390 0010 B 390 0020 B 390 0025 P01 390 0025 R 390 0050 B 390 0050 C 390 0100 C 420 0010 A Page 40 40 40 40 41 41 41 41 41 41 41 41 42 42 42 42 42 42 42 42 42 42 42 42 42 43 43 43 43 43 43 43 43 43 43 37 37 37 37 37 37 37 37 38 38 38 38 38 38 38 38 44 420 0010 B 420 0020 B 420 0025 P01 420 0025 R 420 005
18. 50 mg 3 mL column RapidTrace and ISOLUTE SI 500 mg 3 mL column RapidTrace and ISOLUTE NH2 500 mg 3 mL column RapidTrace and EVOLUTE AX 100 mg 3 mL column RapidTrace and EVOLUTE CX 100 mg 3 mL column RapidTrace and ISOLUTE HCX 200 mg 3 mL column RapidTrace and ISOLUTE EPH 1 459 3 mL column RapidTrace and ISOLUTE HCX 130 mg 3 mL column RapidTrace and ISOLUTE C18 EC 200 mg 3 mL column RapidTrace Part number Industry 902 0020 B 902 0013 B 612 0005 B 460 0050 B 470 0050 B 613 0010 B 611 0010 B 902 0020 B 928 0145 B 902 0013 B 221 0020 B Forensic Forensic Clinical Clinical Environmental Forensic Food Forensic Environmental Clinical Clinical Automated High Throughput Solid Phase Extraction 46 46 26 60 26 26 46 138 46 33 109 Evaporation SPE Dry 96 amp 96 Dual sample Concentrator System SPE Dry 96 Solvent evaporation prior to analysis is a time consuming process during analytical sample preparation and vvith the increasing use of high throughput formats and short analytical cycle times it can become a bottleneck The SPE Dry 96 and SPE Dry 96 Dual Sample Concentrator Systems offer fast efficient solvent Both systems deliver heated gas from above and belovv evaporation from 96 vvell plate formats the plate ensuring rapid reproducible evaporation for a range of commonly
19. 50 mg plate 1 360 0100 P01 ISOLUTE 96 PH 100 mg plate 1 ISOLUTE Array PH Loose Wells Part number Description Quantity 360 0025 R ISOLUTE Array PH 25 mg 1 mL wells 100 360 0050 R ISOLUTE Array PH 50 mg 1 mL wells 100 360 0100 T ISOLUTE Array PH 100 mg 2 mL wells 100 Pre assembled ISOLUTE Array plates are available To order add the suffix P to the equivalent loose well part number Support Documents TN101 Method Development in Solid Phase Extraction using Non polar ISOLUTE SPE Columns for the Extraction of Aqueous Samples TN112 General Approach to the Extraction of Basic Drugs from Biological Fluids using Non polar Non endcapped Sorbents See pages 158 and 159 for additional technical information about ISOLUTE PH Applications Matrix Analytes Retention Mechanism Aqueous Wide polarity range Primary Non polar s 0 5 Endcapped sorbent to minimize secondary silanol interactions S 0 Si CH Chemical structure of phenyl silane and trimethyl silyl group covalently bonded to the surface of a silica particle Ordering Information ISOLUTE PH EC SPE Columns Part number Description Quantity 361 0005 A ISOLUTE PH EC 50 mg 1 mL 100 361 0010 A ISOLUTE PH EC 100 mg 1 mL 100 361 0010 B ISOLUTE PH EC 100 mg 3 mL 50 361 0020 B ISOLUTE PH EC 200 mg 3 mL 50 361 0050 B ISOLUTE PH EC 500 mg 3 mL 50 361 0050 C ISOLUTE PH EC 500 mg 6 mL 30 361 0100 C ISOLUTE PH EC 1 g 6 mL 30 ISOLUTE 96 PH EC Fixed
20. 53 53 53 53 54 54 54 96 54 54 54 54 54 54 54 54 54 54 96 54 54 54 54 54 54 54 96 54 54 54 55 55 55 55 55 55 55 55 55 55 55 55 55 55 56 520 0005 A 520 0010 A 520 0010 B 520 0020 B 520 0025 P01 520 0025 R 520 0050 B 520 0050 C 520 0050 P01 520 0050 R 520 0100 C 520 0100 P01 520 0200 D 530 0005 A 530 0010 A 530 0010 B 530 0020 B 530 0025 P01 530 0050 B 530 0050 C 530 0050 H 530 0050 P01 530 0050 R 530 0100 B 530 0100 C 530 0100 P01 530 0100 T 530 0200 D 532 0002 A 532 0005 A 532 0010 A 532 0010 B 532 0020 B 532 0025 P01 532 0025 R 532 0050 B 532 0050 C 532 0050 H 532 0050 P01 532 0100 B 532 0100 C 532 0100 P01 532 0200 D 533 0002 A 533 0005 A 533 0010 A 533 0010 B 533 0020 B 533 0025 P01 533 0025 R 533 0050 B 533 0050 C 533 0050 H 56 56 56 56 132 56 56 56 56 56 56 56 56 56 57 57 57 57 57 57 57 57 57 57 57 57 57 57 57 58 58 58 58 58 58 58 58 58 58 58 58 58 58 58 59 59 59 59 59 59 59 59 59 59 Part Number Page 610 0050 C 26 714 0010 A 63 902 0013 H 46 123 533 0050 P01 59 611 0005 B 26 27 714 0020 A 63 128 130 131 533 0050 R 59 132 151 714 0020 B 63 902 0020 B 46 109 533 0100 B 59 611 0005 26 96 714 0050 63 130 131 533 0100 59 611 0010 26 109 714 0050 63 902 0025 01 46 533 0100 P01 59 151 714 0100 63 902 0025 R 46 533 0100 T 59 611 0010 BG 26 96 714 0100 C 63 902 0030 B
21. Aflatoxins from Cereals IST1076 Extraction of Acrylamide from Cooked Foodstuffs See pages 162 and 163 for additional technical information about ISOLUTE Multimode Applications Matrix 0 Analytes Retention Mechanisms Aqueous Aqueous Anionic Primary Weak anion exchange Chemical structure of NH2 silane covalently bonded to the surface of a silica particle Ordering Information ISOLUTE NH2 SPE Columns Part number Description Quantity 470 0002 A ISOLUTE NH2 25 mg 1 mL 100 470 0005 A ISOLUTE NH2 50 mg 1 mL 100 470 0005 G ISOLUTE NH2 50 mg 10 mL 50 470 0010 A ISOLUTE NH2 100 mg 1 mL 100 470 0010 B ISOLUTE NH2 100 mg 3 mL 50 470 0010 G ISOLUTE NH2 100 mg 10 mL 50 470 0020 B ISOLUTE NH2 200 mg 3 mL 50 470 0050 B ISOLUTE NH2 500 mg 3 mL 50 470 0050 C ISOLUTE NH2 500 mg 6 mL 30 470 0050 H ISOLUTE NH2 500 mg 10 mL 50 470 0100 C ISOLUTE NH2 1 g 6 mL 30 470 0200 C ISOLUTE NH2 2 g 6 mL 30 470 0200 D ISOLUTE NH2 2 g 15 mL 20 ISOLUTE 96 NH2 Fixed Well Plates Part number Description Quantity 470 0050 P01 ISOLUTE 96 NH2 50 mg plate 1 470 0100 PO1 ISOLUTE 96 NH2 100 mg plate 1 ISOLUTE Array NH2 Loose Wells Part number Description Quantity 470 0025 R ISOLUTE Array NH2 25 mg 1 mL wells 100 Pre assembled ISOLUTE Array plates are available To order add the suffix P to the equivalent loose well part number Support Documents TN102 Method Development in Solid Phase Extraction using Polar ISOLUTE SPE Columns for the Extraction
22. Columns for Acrylamide Clean up Determination of acrylamide in cooked foodstuffs such as fried potato products can be problematic due to the highly polar water soluble nature of the analyte and the complexity of the sample matrix ISOLUTE Multimode SPE columns provide for extremely effective clean up of aqueous food extracts allowing acrylamide determina tion by LC MS See Application Note IST1076 Extraction of Acrylamide from Cooked Foods Visit www biotage com applications to download Ordering Information ISOLUTE Multimode Description Quantity Part number ISOLUTE Multimode 300 mg 3 mL 50 904 0030 B ISOLUTE ENV SPE Columns for Extraction of Highly Polar Compounds ISOLUTE ENV is an extremely retentive SPE sorbent used for extraction of highly polar pesticides and herbicides from aque ous samples Unlike other polymeric SPE sorbents it is able to retain highly water soluble compounds such as acrylamide from aqueous food extracts Ordering information for ISOLUTE ENV SPE columns can be found on page 30 See Application Note IST1082 Extraction and Clean up of Acrylamide from Foodstuffs ISOLUTE Filtration Columns and Depth Filter Reservoirs When extracting samples containing particulate material it is often necessary to pre filter the sample prior to SPE This prevents blocking of the SPE column ISOLUTE Filtration Columns contain two 20 um polyethylene frits and can be connected directly above the extraction c
23. Description Quantity 520 0025 P01 ISOLUTE 96 CBA 25 mg plate 1 520 0050 P01 ISOLUTE 96 CBA 50 mg plate 1 520 0100 P01 ISOLUTE 96 CBA 100 mg plate 1 ISOLUTE Array CBA Loose Wells Part number Description Quantity 520 0025 R ISOLUTE Array CBA 25 mg 1 mL wells 100 520 0050 R ISOLUTE Array CBA 50 mg 1 mL wells 100 Pre assembled ISOLUTE Array plates are available To order add the suffix P to the equivalent loose well part number Support Documents TN108 Method Development in Solid Phase Extraction using ISOLUTE CBA SPE Columns for the Extraction of Aqueous Samples See pages 166 and 167 for additional technical information about ISOLUTE CBA Applications Matrix Analytes Retention Mechanisms Primary Strong cation exchange Aqueous Cationic Secondary Non polar Non polar Primary TE Non polar Cationic cation exchange Chemical structure of benzenesulfonic acid functional group covalently bonded to the surface of a silica particle Ordering Information ISOLUTE SCX SPE Columns Part number Description Quantity 530 0005 A ISOLUTE SCX 50 mg 1 mL 100 530 0010 A ISOLUTE SCX 100 mg 1 mL 100 530 0010 B ISOLUTE SCX 100 mg 3 mL 50 530 0020 B ISOLUTE SCX 200 mg 3 mL 50 530 0050 B ISOLUTE SCX 500 mg 3 mL 50 530 0050 C ISOLUTE SCX 500 mg 6 mL 30 530 0050 H ISOLUTE SCX 500 mg 10 mL 50 530 0100 B ISOLUTE SCX 1 g 3 mL 50 530 0100 C ISOLUTE SCX 1 g 6 mL 30 530 0200 D ISOLUTE SCX 2 g 15 mL 20 ISOLUTE 96 SCX Fixed Well Plates P
24. EVOLUTE CX 100 mg 3 mL Tabless EVOLUTE CX 100 mg 10 mL XL EVOLUTE CX 200 mg 3 mL EVOLUTE CX 200 mg 6 mL EVOLUTE CX 500 mg 6 mL EVOLUTE CX 500 mg 6 mL Tabless EVOLUTE WCX 25 mg 1 mL EVOLUTE WCX 50 mg 3 mL EVOLUTE WCX 100 mg 3 mL EVOLUTE WCX 100 mg 3 mL XL EVOLUTE WCX 200 mg 6 mL EVOLUTE WCX 500 mg 6 mL EVOLUTE AX 25 mg 1 mL EVOLUTE AX 25 mg 1 mL Tabless EVOLUTE AX 50 mg 3 mL EVOLUTE AX 100 mg 3 mL EVOLUTE AX 100 mg 3 mL Tabless EVOLUTE AX 100 mg 10 mL XL EVOLUTE AX 200 mg 6 mL EVOLUTE AX 500 mg 6 mL EVOLUTE AX 500 mg 6 mL Tabless EVOLUTE WAX 25 mg 1 mL EVOLUTE WAX 50 mg 3 mL EVOLUTE WAX 100 mg 3 mL EVOLUTE WAX 100 mg 10 mL XL EVOLUTE WAX 200 mg 6 mL EVOLUTE WAX 500 mg 6 mL Other tabless columns are available contact Biotage for details Quantity 100 100 100 50 50 50 50 50 50 50 30 30 100 100 100 100 50 50 50 50 50 50 30 30 30 100 50 50 50 30 30 100 100 50 50 50 50 30 30 30 100 50 50 50 30 30 EVOLUTE Advanced Polymeric Sorbents for LC MS MS Applications Applications 35 Part number Industry number AN702 Basic Drugs Plasma EVOLUTE CX 601 0025 P01 Bioanalytical 25 25 mg fixed well plate AN739 Catecholamines Plasma EVOLUTE WCX 612 0005 B Clinical 26 50 mg 3 mL column AN745 Catecholamines Plasma EVOLUTE VVCX 602 0025 PO1 Clinical 25 25 mg fixed vvell plate AN719 Cocaine and Biological EVOLUTE CX 601 0025 P01 Forensic 25 m
25. ISOLUTE SPE columns and 96 well SPE plates 30 45 O Octadecyl see C18 C18 EC MFC18 Octyl see C8 C8 EC Oil amp Grease column O amp G 139 P Part numbering system SPE column 12 Part Number Index 193 196 PAH SPE columns 138 PE AX Pre equilibrated anion exchanger 96 well SPE plates 55 SPE columns 55 PH PH EC Phenyl 96 well SPE plates 42 43 SPE columns 42 43 Phenyl see PH PH EC 42 43 Polar sorbents 66 168 171 Polar SPE columns and 96 well SPE plates 60 63 Polyethylene frits 88 Polypropylene reservoirs 87 Polystyrene divinylbenzene see EVOLUTE ABN 101 and ENV Primary secondary amine see PSA PRESSURE 48 and PRESSURE 96 Positive pressure manifolds Product selection guide 94 96 154 155 Protein precipitation plates 82 83 119 Propylsulfonic acid see SCX 2 PRS PSA Primary secondary amine 96 well SPE plates Bulk sorbent SPE columns PTFE adapters PTFE extraction caps PTFE frits PTFE needles VacMaster PTFE stopcocks VacMaster PTFE stopcock needles VacMaster PTFE stopcocks for universal use Q Quality issues Quaternary amine see PE AX and SAX QuEChERS QuickStart Guide to SPE QuickStart Guide to SLE R Rack Gravity RapidTrace Rack for VacMaster Reservoirs S SAX Quaternary amine 96 well SPE plates SPE columns SAX PSA SPE columns SCX Benzenesulfonic acid 96 well SPE plates SPE columns 53 66 53 86 14
26. Primary Non polar and Cationic and strong cation exchange Aqueous Si O Si CH CHs TSOLUTE HCX 5 has reduced non polar character compared to ISOLUTE HCX amp HCX 3 ISOLUTE HCX 5 combines C4 Butyl and sulfonic acid functionalities Ordering Information ISOLUTE HCX 5 SPE Columns Part number Description Quantity 906 0002 A ISOLUTE HCX 5 25 mg 1 mL 100 906 0005 A ISOLUTE HCX 5 50 mg 1 mL 100 906 0010 A ISOLUTE HCX 5 100 mg 1 mL 100 906 0010 G ISOLUTE HCX 5 100 mg 10 mL 50 906 0013 H ISOLUTE HCX 5 130 mg 10 mL 50 ISOLUTE 96 HCX 5 Fixed Well Plates Part number Description Quantity 906 0025 P01 ISOLUTE 96 HCX 5 25 mg plate 1 906 0100 P01 ISOLUTE 96 HCX 5 100 mg plate 1 ISOLUTE Array HCX 5 Loose Wells Part number Description Quantity 906 0025 R ISOLUTE Array HCX 5 25 mg 1 mL wells 100 906 0100 T ISOLUTE Array HCX 5 100 mg 2 mL wells 100 Pre assembled ISOLUTE Array plates are available To order add the suffix P to the equivalent loose well part number Support Documents TN116 Generic Method for the Extraction of Basic Drugs from Biological Fluids using ISOLUTE Mixed mode SPE Columns and 96 well Plates See pages 162 and 163 for additional technical information about ISOLUTE HCX 5 SOLUTE HCA Q Si O Si CH s COO Applications Matrix Analytes Retention Mechanisms Non polar and Aqueous quaternary Primary Non polar and amine or weak cation exchange polybasic Si O S
27. Rack for 15 mL Conical Centrifuge Tubes P N C44941 125 tubes included Tube Rack 15 mL Autotrace tubes with conical bottom C47816 Tube Racks 10 mL conical tubes C47811 Tube Rack 1 5 2 0 mL flip cap microcentrifuge tubes Tube Rack 1 5 2 0 mL screw cap microcentrifuge tubes Tube Rack GC vials Tube Rack IChem Vials Part num C103202 C103203 C103263 C103264 C103186 C103187 C103188 C103189 C103190 C103192 C103193 C103194 C103196 C103197 C103198 C103199 C103200 C103201 C44139 C44283 C45269 C44577 C45286 C44282 C48950 C44880 C47818 C47820 C48928 C48929 C59546 C61345 ber Applications and Products for Bioanalysis Bioanalytical Applications and Products for Bioanalysis In today s bioanalytical laboratory reliable high throughput sample preparation techniques are essential IST Sample Preparation Products from Biotage provide a range of solutions matching the throughput requirements and sample clean up needs of the drug development process Biotage is dedicated to developing sample preparation products that solve the daily challenges faced by bioanalytical scientists For example our product development program is strongly influenced by the need to reduce matrix effects in LC MS without compromising speed or simplicity of sample preparation Generic methodologies optimized for extraction of small volumes of biological fluids are available for most products minimizing metho
28. Upon inhalation both passive and active NNK is rapidly metabolized to NNAL 4 methylnitrosamino 1 3 pyridyl 1 butanol The extraction and quantification of NNAL in urine is therefore a useful biomarker when assessing exposure to tobacco smoke The analysis of NNAL however is challenging due to the detection limits required especially in the monitoring of nonsmokers exposed to passive smoking where detection limits are below 10 ng L lt 10 ppt Such detection limits are not obtainable using conventional SPE and procedures are often laborious and time consuming The application note for AFFINILUTE MIP NNAL describes the extraction of NNAL from urine with detection limits down to 5 pg mL and recoveries of greater than 90 Description Quantity Part number AFFINILUTE MIP NNAL 25 mg 10 mL 50 M06 0002 G AFFINILUTE MIP NNAL 25 mg 3 mL 50 M06 0002 B AFFINILUTE MTP Triazines Triazine compounds are used as the basis for various herbicides AFFINILUTE MTP Triazine provides excellent extraction and selectivity for a spectrum of 10 triazines enabling lovver detection limits vvith lovver sample volumes The 10 triazines and triazine metabolites that can be extracted are atrazine simazine propazine cyanazine sebuthylazine deisopropylatrazine deethylatrazine deethylterbutylazine prometon and hydroxyterbutylazine Description Quantity Part number AFFINILUTE MIP Triazines 25 mg 10 mL 50 08 0002 l a E M a j 4 F
29. Well Plates Part number Description Quantity 361 0025 P01 ISOLUTE 96 PH EC 25 mg plate 1 ISOLUTE Array PH EC Loose Wells Part number Description Quantity 361 0025 R ISOLUTE Array PH EC 25 mg 1 mL wells 100 361 0050 R ISOLUTE Array PH EC 50 mg 1 mL wells 100 Pre assembled TSOLUTE Array plates are available To order add the suffix P to the equivalent loose vvell part number Support Documents TN101 Method Development in Solid Phase Extraction using Non polar ISOLUTE SPE Columns for the Extraction of Aqueous Samples See pages 158 and 159 for additional technical information about ISOLUTE PH EC po d l HE r e EW y Y ESAS Applications Matrix Analytes Retention Mechanisms Primary Non polar Aqueous Wide polarity range Secondary Weak cation exchange Chemical structure of cyanopropyl silane covalently bonded to the surface of a Silica particle Ordering Information ISOLUTE CN SPE Columns Part number Description Quantity 420 0010 A ISOLUTE CN 100 mg 1 mL 100 420 0010 B ISOLUTE CN 100 mg 3 mL 50 420 0020 B ISOLUTE CN 200 mg 3 mL 50 420 0050 B ISOLUTE CN 500 mg 3 mL 50 420 0050 C ISOLUTE CN 500 mg 6 mL 30 420 0100 C ISOLUTE CN 1 g 6 mL 30 ISOLUTE 96 CN Fixed Well Plates Part number Description Quantity 420 0025 P01 ISOLUTE 96 CN 25 mg plate 1 420 0100 P01 ISOLUTE 96 CN 100 mg plate 1 ISOLUTE Array CN Loose Wells Part number Description Quantity 420 0025 R ISOLUTE Array CN 25 mg 1 mL
30. amp weak anion exchange 30 50 40 NR2 NR3 ISOLUTE Silica Based Mixed Mode Sorbents ISOLUTE HCX Octyl C8 non endcapped and strong cation exchange S037 ISOLUTE HCX 3 Octadecyl C18 non endcapped and strong cation exchange S037 ISOLUTE HCX 5 Butyl C4 non endcapped and strong cation exchange S037 ISOLUTE HCX Q Octyl C8 non end capped and weak cation exchange ISOLUTE HAX Octyl C8 non endcapped and strong cation exchange NR ISOLUTE Octadecyl C18 non endcapped strong cation exchange S0 and Multimode strong cation exchange NR3 162 Mixed Mode Sorbents Application Chemistry Ordering Data Sheets Information Page Extraction of basic analytes from biological fluids and other aqueous samples using a generic TN139 amp 141 22 27 method Can also be used to fractionate basic neutral and acidic compounds Extraction of basic analytes from biological fluids and other aqueous samples Acidic elution TN145 22 27 conditions can be used Also suitable for quaternary amines and other strongly basic analytes Extraction of acidic analytes from biological fluids and other aqueous samples using a TN144 22 27 generic method Extraction of strongly acidic analytes e g phosphates sulphates from biological fluids and TN146 22 27 other aqueous samples using a generic method gt N o The first choice sorbent for extracting drugs of abuse f
31. and other automated SPE systems Other tabless columns are available contact Biotage for details L Applications Matrix Analytes Retention Mechanisms Si O i R SO H Non polar Primary Non polar A queous and Cationic and strong cation exchange Si O Si CH CH ISOLUTE HCX 3 has increased non polar character compared to ISOLUTE HCX 3 combines C18 Octadecyl ISOLUTE HCX and HCX 5 and sulfonic acid functionalities Ordering Information ISOLUTE HCX 3 SPE Columns Part number Description Quantity 905 0002 A ISOLUTE HCX 3 25 mg 1 mL 100 905 0005 A ISOLUTE HCX 3 50 mg 1 mL 100 905 0010 A ISOLUTE HCX 3 100 mg 1 mL 100 ISOLUTE 96 HCX 3 Fixed Well Plates Part number Description Quantity 905 0025 P01 ISOLUTE 96 HCX 3 25 mg plate 1 905 0100 P01 ISOLUTE 96 HCX 3 100 mg plate 1 ISOLUTE Array HCX 3 Loose Wells Part number Description Quantity 905 0025 R ISOLUTE Array HCX 3 25 mg 1 mL wells 100 905 0100 T ISOLUTE Array HCX 3 100 mg 2 mL wells 100 Pre assembled ISOLUTE Array plates are available To order add the suffix P to the equivalent loose well part number Support Documents TN116 Generic Method for the Extraction of Basic Drugs from Biological Fluids using ISOLUTE Mixed mode SPE Columns and 96 well Plates See pages 162 and 163 for additional technical information about ISOLUTE HCX 3 Applications Matrix Analytes Retention Mechanisms Na Si O i R SO H Non polar
32. and reconstitute with an alternative solvent If you need to evaporate acidic solutions Biotage offer Teflon coated nozzles instead of stainless steel nozzles to prevent corrosion Compatible with Dionex ASE For volumes greater than 30 mL Biotage offers the ASE version of the TurboVap LV compatible with Dionex ASE 200 columns This has 24 sample positions and comes with a rack that has a removable shelf allowing either 40 mL or 60 mL Dionex tubes to be used Key Applications The TurboVap LV can be used in environmental forensic clinical chemistry food and pharmaceutical laboratories for the concentration of solvents following solid phase extraction clean up of drug samples or pesticide extracts The TurboVap LV can also be used in conjunction with the RapidTrace Automated SPE workstation that collects extracts in 12 mm x 75 mm 13 mm x 100 mm or 16 mm x 100 mm test tubes TurboVap II Volume Range 50 mL to 200 mL The TurboVap II is ideal for evaporation of large volumes after performing liquid liquid extractions The instrument has 6 sample positions and uses special glass tubes with either a 1 mL or 0 5 mL end point The glassware and optical sensors automatically stop gas flow when evaporation has reached its end point and allows for concentration of samples without the risk of drying thus preventing the loss of volatile compounds TurboVap II takes up only a fraction of the bench space used by conventional rotary evapor
33. are available on each of the instruments along with information on the test tube sizes evaporation rate guides specifications and some application notes For further information please visit www biotage com PAPA UIIIIC Description TurboVap 500 100 120V 50 60Hz TurboVap 500 200 220V 50 60Hz TurboVap 96 120 VAC TurboVap 96 220 VAC TurboVap II 200 mL with 0 5 mL Endpoint Stem 120V TurboVap II 200 mL with 1 0 mL Endpoint Stem 115V TurboVap II 50 mL with 0 5 mL Endpoint Stem 110V TurboVap II 50 mL with 1 0 mL Endpoint Stem 110V TurboVap II 200 mL with 0 5 mL Endpoint Stem 220V TurboVap II 200 mL with 1 0 mL Endpoint Stem 220V TurboVap II 50 mL with 0 5 mL Endpoint Stem 220V TurboVap II 50 mL with 1 0 mL Endpoint Stem 220V TurboVap II 40 mL Dionex ASE Vial Compatible 110V TurboVap II 40 mL Dionex ASE Vial Compatible 220V TurboVap LV without Racks 120V TurboVap LV without Racks 230V TurboVap LV ASE 40 60 mL vial 110V TurboVap LV ASE 40 60 mL vial 230V Tube Rack for 16 x 100 mm tubes P N C40708 250 tubes included Tube Rack 16 x 100 mm Prelude compatible C40708 Tube Rack for 16 x 125 mm tubes P N C45273 250 tubes included Tube Rack for 12 x 75 mm tubes P N C44651 250 tubes included Tube Rack for 13 x 100 mm tubes P N C40707 250 tubes included Tube Rack for 20 x 150 mm tubes P N C40709 250 tubes included Tube Rack for 10 x 75 mm tubes P N C48985 250 tubes included Tube
34. column ISOLUTE SLE 200 Supported Liquid Extraction plate and ISOLUTE SLE 1 mL column ISOLUTE HCX 200 mg 10 mL column ISOLUTE HCX 130 mg 1 g or 3 mL columns ISOLUTE THC 100 mg 3 mL column ISOLUTE C8 100 mg 1 mL column ISOLUTE THC 100 mg 1 mL column ISOLUTE HAX 200 mg 10 mL column ISOLUTE SLE 200 Supported Liquid Extraction plate ISOLUTE HCX 130 mg 3 mL columns ISOLUTE 101 500 mg 6 mL columns Part number 603 0025 P01 613 0010 B 902 0013 H 601 0025 P01 902 0020 B 902 0013 A 820 0200 P01 820 0140 C 902 0020 H 902 0013 A 902 0013 B 292 0010 B 290 0010 A 292 0010 A 903 0020 H 820 0200 P01 902 0013 B 101 0050 C Visit the Sample Preparation Application Database at www biotage com applications 25 26 46 128 25 46 46 128 76 76 46 46 128 46 128 129 35 129 50 76 46 128 31 131 Forensic and Clinical Applications and Products for Forensic and Clinical Analysis Clinical Applications Application Analyte Matrix Format Part number number AN751 Benzodiazepines Urine ISOLUTE SLE 200 Supported Liquid Extraction plate or 820 0200 PO1 76 ISOLUTE SLE 1 mL column 820 0140 C 76 AN707 B Blockers Biological AFFINILUTE MIP Fluids 25 mg 3 mL or M18 0002 B 17 127 137 25 mg 10 mL column M18 0002 G 17 127 137 AN739 Catecholamines Plasma EVOLUTE VVCX 612 0005 B 26 50 mg 3 mL column AN744 Catec
35. foodstuffs The application note for AFFINILUTE MTP Fluoroquinolones describes the extraction of fluoroquinolones specifically from bovine kidney and is applicable to many tissues for food testing Description Quantity Part number AFFINILUTE MIP Fluoroquinolones 25 mg 3 mL 50 M69 0002 B AFFINILUTE MIP TSNAs Tobacco specific nitrosamines TSNAs are created through the burning curing and fermentation of tobacco leaf In 1989 the US Surgeon General provided a list of carcinogens found in tobacco products Among that list were nine nitrosamines that can be found in chewing smoking and snuff tobacco These TSNAs are highly carcinogenic and have been linked to lung oral esophageal cervical and liver cancer The monitoring of humans for exposure to tobacco smoke active or passive is an important clinical test Nicotine is metabolized in the tobacco curing process to the TSNAs NNK and NNN NNK is further metabolized in the body to NNAL Because TSNAs are only found in tobacco products their characterization is invaluable in the study of tobacco s cancerous nature The application note for AFFINILUTE MIP TSNAs describes the extraction of TSNAs from urine Description Quantity Part number AFFINILUTE MIP TSNAs 50 mg 10 mL 50 M21 0005 G AFFINILUTE MIP TSNAs 50 mg 3 mL 50 M21 0005 B AFFINILUTE MIP NNAL NNK 4 methylnitrosamino 1 3 pyridyl 1 butanone is a tobacco specific nitrosamine which is present in significant quantities in tobacco smoke
36. mL 50 902 0013 A ISOLUTE HCX 130 mg 1 mL 100 902 0013 B ISOLUTE HCX 130 mg 3 mL 50 902 0013 BG ISOLUTE HCX 130 mg 3 mL Tabless 50 902 0013 C ISOLUTE HCX 130 mg 6 mL 30 902 0013 CG ISOLUTE HCX 130 mg 6 mL Tabless 30 902 0013 H ISOLUTE HCX 130 mg 10 mL 50 902 0020 B ISOLUTE HCX 200 mg 3 mL 50 902 0020 H ISOLUTE HCX 200 mg 10 mL 50 902 0030 B ISOLUTE HCX 300 mg 3 mL 50 902 0030 C ISOLUTE HCX 300 mg 6 mL 30 902 0030 CG ISOLUTE HCX 300 mg 6 mL Tabless 30 902 0030 H ISOLUTE HCX 300 mg 10 mL 30 ISOLUTE 96 HCX Fixed Well Plates Part number Description Quantity 902 0025 P01 ISOLUTE 96 HCX 25 mg plate 1 902 0050 PO1 ISOLUTE 96 HCX 50 mg plate 1 902 0100 PO1 ISOLUTE 96 HCX 100 mg plate 1 ISOLUTE Array HCX Loose Wells Part number Description Quantity 902 0025 R ISOLUTE Array HCX 25 mg 1 mL wells 100 902 0050 R ISOLUTE Array HCX 50 mg 1 mL wells 100 902 0100 T ISOLUTE Array HCX 100 mg 2 mL wells 100 Pre assembled ISOLUTE Array plates are available To order add the suffix P to the equivalent loose well part number Support Documents TN116 Generic Method for the Extraction of Basic Drugs from Biological Fluids using ISOLUTE Mixed mode SPE Columns and 96 well Plates TN125 Method Development in Solid Phase Extraction using ISOLUTE HCX for the Extraction of Drugs from Biological Fluid Samples See pages 162 and 163 for additional technical information about ISOLUTE HCX Tabless columns for use with Pressure Positive Pressure Manifolds
37. precise temperature control increases the speed of evaporation by a factor of 10 compared with other techniques See page 113 1 Point Support Customer Support Method Development and Troubleshooting Advice Biotage is ready to assist you with our team of Analytical Chemists who have many years of experience in providing practical theoretical and technical knovvledge relating to sample preparation techniques If you need help choosing the best sample preparation method for your application or vvant to solve an existing sample preparation problem please contact your local 1 Point Support team who will ensure you receive fast advice from our sample preparation experts Technical Information Throughout this catalog vve highlight chemistry data sheets and application notes that provide detailed information on sample preparation techniques and SPE sorbents SPE Quickstart Guide Use our NEW QuickStart Guide on page 173 for an introduction to SPE Supported Liquid Extraction User s Guide ISOLUTE SLE supported liquid extraction is a quick easy and cost effective sample preparation solution for analytical chemistry The Biotage SLE User Guide provides a simple 4 step method development guide which outlines the efficient 3 step sample procedure of loading your sample wait five minutes and elute To request a copy please visit www biotage com Biotage Sample Preparation Application Database Biotage s
38. proteins from biological fluid samples Non selective technique suitable for extraction of a broad range of compounds extracts can give relatively high levels of ion suppression An easily automated technique analogous to liquid liquid extraction Allows removal of proteins and phospholipids from bioanalytical fluid samples Depending on sorbent choice can provide high analyte recoveries of a broad range of compounds or extremely selective extraction of specific compounds with very clean extracts Highly selective giving reduced matrix effects and ion suppression as interferences can be washed from the columns using stringent conditions Bioanalytical Applications and Products for Bioanalysis ISOLUTE PPT Protein Precipitation Plates ISOLUTE PPT Protein Precipitation Plates provide effective high throughput protein removal from plasma samples The simple filtration based procedure is easily automated and is applicable to a wide range of drugs and metabolites See page 81 83 for more details and ordering information ISOLUTE SLE Supported Liquid Extraction Plates and Columns ISOLUTE SLE Supported Liquid Extraction Plates are optimized for reliable high throughput extraction of 96 samples simultaneously using liquid handling systems Up to 400 uL of aqueous sample can be extracted per extraction well For applications using ISOLUTE SLE relevant to the bioanalytical market please visit w
39. samples e Parallel processing for increased sample throughput e Easy to set up and use with just a gas source e Universal designs accommodate all 96 well plates or 1 mL 3 mL and 6 mL SPE columns e Built in safety features ensure ease of use and safe operation Uniform Flow Unlike vacuum manifolds in which faster flowing wells and columns dry before the processing run is completed the independent ports of the PRESSURE manifolds deliver consistent output This allows the same motive force to be applied to every cartridge and well regardless of the contents Ideal for Viscous Samples Biotage PRESSURE Positive Pressure manifolds allow for gas pressures of up to 100 psi enabling greater pressure and force to be used with viscous samples Smooth and immediate flow responses are achieved by adjusting gas pressure up or down through two regulators and a rotometer PRESSURE 96 Accommodates all 96 Well Plate Formats The self adjusting upper manifold of the PRESSURE 96 manifold is compatible with all 96 well plate formats including the popular 1 mL and 2 mL modular Array well formats without the need to purchase supplementary gaskets Biotage collection plates are recommended for the most consistent and reliable results PRESSURE 48 for Column Processing The same self adjusting technology utilized in the PRESSURE 96 manifold allows the PRESSURE 48 to process all columns up to 6 mL size without the need to purchase additional gaskets The
40. samples unattended Extracts are freshly prepared when you return e Modular Design Allows for up to 10 Linked Units The Modular design allows users to start with one module and expand up to 10 linked units Modules within a group can run different methods independently and if one module is shut down for operator attention other units remain operational so that sample throughput is not halted e Accelerated Methods Development and Optimization Utilizing the easy to use interface it is simple to implement a structured rational strategy for optimum SPE conditions in experiments e Readily Transferable Methods Methods stored in the RapidTrace controller can be instantly recalled and transferred to other units with 100 confidence RapidTrace Automated High Throughput Solid Phase Extraction e Positive Pressure Syringe Displacement Positive pressure displacement allows for accurate processing and improves reproducibility in flow rate sensitive methods e Safety and Security Enclosed working parts ensure operator safety and reduced exposure to solvents and hazardous compounds Additionally password protected control software limits access to methods ensuring method security Combined with magnetically encoded racks run initiation is simplified and the possibility of loading an incorrect procedure is reduced e 8 Solvent Inlet Lines Allows for more flexible use of solvents and provides an excellent system for method development e Seg
41. step can result in dirty extracts Flow rates should be specified in all protocols to ensure trouble free transfer from one analyst or lab oratory to another See Table 4 page 183 for typical flow rates used during method development Step 1 Sample pre treatment Sample pre treatment may simply be a dilution of the sample with an appropriate solvent in order to reduce viscosity or it could involve addition of a buffer to control the pH of the sample prior to retention by non polar mixed mode or ion exchange sorbents a Ensure analytes are in solution and available for interaction with the sorbent If the sample contains particulate matter to which the analytes absorb it is essential to first desorb the analytes from the particulates before applying the sample to the SPE column Similarly if analytes are bound to large molecules in the sample e g drugs bound to proteins present in biological fluids this binding must be disrupted to achieve high extraction efficiencies This can usually be achieved by the addition of a small percentage organic solvent or pH adjustment of the sample b Filter or otherwise remove e g centrifuge sample particulates to prevent column blockage c Non polar sorbents i Enhance retention of ionizable analytes by adjusting sample pH to suppress ionization of the analyte For acidic analytes adjust pH to 2 pH units below the pK of the analyte For basic analytes adjust pH to 2 pH units above the
42. that some applications might require a different product or alternative configuration If your application requires a sample preparation product which is not listed in this catalog we can supply an alternative under our Custom Manufacturing Service Some of the options are described here As with all custom manufacturing minimum order quantities may apply Typical SPE Column Configurations The SPE sorbents listed in this catalog can be packed into non standard column or plate formats As a guide this table lists the typical sorbent bed mass for each reservoir volume Note not all sorbents are available in all sizes Bed mass Reservoir Biotage s XL columns have the same sorbent bed dimensions as 1 or 3 mL column but have an integral 10 mL reservoir Tabless Columns 1 3 and 6 mL columns are available in tab less format for use in PRESSURE positive pressure manifolds and with other liquid handling systems such as Gilson 215 systems Simply add a SG suffix to the part number for tab less formats There is no extra charge for tabless cartridges ASPEC Caps 1 3 and 6 mL columns standard or tabless can be supplied pre fitted with ASPEC caps for processing on Gilson ASPEC and 215 systems Filtration Options The standard frit material used in the mafority of our sample preparation products is sintered polyethylene vvith 10 or 20 um nominal porosity We can also supply sintered stainless steel or PTFE frits wi
43. use high ionic strength buffer gt 0 1M or acidi fied methanol QuickStart Guide to SPE Table 4 Recommended flow rates for method development Once the optimum chemistry has been established optimize flow rate to maximize productivity Increase flow until breakthrough is observed The final flow rate should be set at 10 20 lower than the breakthrough limit Table 5 Recommended volumes for method development Column solvation 250 500 uL Column equilibration 250 500 uL a Application specific based on sorbent capacity and analyte Sample application m concentration in sample Interference elution 250 500 HL 250 uL 1 mL dependEnt on choice of elution solvent Analyte elution To minimize elution volume apply 2 x X 2 mL aliquots including a soak step rather than 1 x X mL aliquot Note 1 bed volume is approximately 120 uL 100 mg sorbent Method optimization process In order to optimize an SPE method the following steps should be followed 1 Select desired retention mechanism e Non polar e Polar e Ion exchange e Mixed mode 2 Using the above guidelines screen sorbents with the selected retention mechanism for retention of standards from a clean matrix similar to the sample type For example if assay is to be developed for extraction of drugs from an aqueous biological fluid first evaluate retention of standards from aqueous buffer 3 Screen a range of elution solvents for elu
44. used solvents The systems are ideal for solvent evaporation following SPE supported liquid extraction SLE and protein precipitation applications SPE Dry 96 and SPE Dry 96 Dual systems are supplied vvith removable stainless steel needles For applications that use aggressive solvents and buffers PTFE coated needles are available Features and Advantages e Fast efficient solvent evaporation from 96 vvell plates e Rapid equilibration and precise temperature control e Compact size small footprint e Single and dual plate options e Also compatible with 48 well and 384 well formats e PTFE coated needle options for added solvent resistance e 384 well heads for use with common plate sizes are available Ordering Information SPE Dry 96 Sample Concentrator System 112 Description SPE Dry 96 Sample Concentrator System 110 V USA SPE Dry 96 Sample Concentrator System 240 V UK SPE Dry 96 Sample Concentrator System 240 V Europe SPE Dry 96 Sample Concentrator System 110 V Japan SPE Dry 96 Dual Sample Concentrator System Description SPE Dry 96 Dual Sample Concentrator System 110 V USA SPE Dry 96 Dual Sample Concentrator System 240 V UK SPE Dry 96 Dual Sample Concentrator System 240 V Europe SPE Dry 96 Dual Sample Concentrator System 110 V Japan Part number SD 9600 DHS NA SD 9600 DHS UK SD 9600 DHS EU SD 9600 DHS JP Part number SD2 9600 DHS NA SD2 9600 DHS UK SD2 9600 DHS EU SD2 9600 DHS JP
45. v v Plasma Dilute with ammonium hydroxide 2 1 3 v v Urine Dilute with ammonium acetate 0 05M pH 7 1 3 v v Dilute with formic acid 2 1 3 v v Dilute with formic acid pH 2 2 1 1 v v Dilute with ammonium acetate 0 05M pH 6 1 1 v v Dilute with ammonium acetate 0 05M pH 7 1 4 v v pH control required to suppress ionization of acidic and basic compounds See previous section of Guide on extraction of basic compounds 1 Ammonium acetate 0 05M pH 6 followed by 2 Methanol 1 Ammonium hydroxide 5 v v followed by 2 Methanol 1 Ammonium acetate 0 05M pH 7 followed by 2 Methanol 1 Ammonium acetate 0 05M pH 7 methanol 95 5 v v followed by 2 Methanol 1 Ammonium acetate 0 05M pH 7 methanol 95 5 v v followed by 2 Methanol 1 Formic acid 2 v v followed by 2 Methanol 1 Ammonium acetate 0 1M pH 7 0 followed by 2 methanol water 1 1 v v 1 Ammonium acetate 0 05M pH 6 followed by 2 Acetic acid 1 M followed by 3 Methanol Methanol ammo nium acetate 0 05M pH7 0 20 80 v v Water or buffer add up to 40 methanol or acetonitrile to remove more polar interferences Check for analyte breakthrough Maintain pH control Methanol ammonium hydroxide 95 5 v v Methanol formic acid 98 2 v v Methanol formic acid 98 2 v v Methanol formic acid 98 2 v v
46. wells 100 420 0100 T ISOLUTE Array CN 100 mg 2 mL wells 100 Pre assembled ISOLUTE Array plates are available To order add the suffix P to the equivalent loose well part number Support Documents TN101 Method Development in Solid Phase Extraction using Non polar ISOLUTE SPE Columns for the Extraction of Aqueous Samples TN102 Method Development in Solid Phase Extraction using Polar ISOLUTE SPE Columns for the Extraction of Non aqueous Samples TN112 General Approach to the Extraction of Basic Drugs from Biological Fluids using Non polar Non endcapped Sorbents See pages 158 and 159 for additional technical information about ISOLUTE CN Applications Matrix Analytes Retention Mechanisms S 0 cH CN Q Si O Si CH Wide polarity range Primary Non polar Endcapped sorbent to minimize secondary silanol interactions Chemical structure of cyanopropyl silane and trimethyl silyl group covalently bonded to the surface of a silica particle Ordering Information ISOLUTE CN EC SPE Columns Part number Description Quantity 421 0005 A ISOLUTE CN EC 50 mg 1 mL 100 421 0010 A ISOLUTE CN EC 100 mg 1 mL 100 421 0010 B ISOLUTE CN EC 100 mg 3 mL 50 421 0020 B ISOLUTE CN EC 200 mg 3 mL 50 421 0050 B ISOLUTE CN EC 500 mg 3 mL 50 421 0100 C ISOLUTE CN EC 1 g 6 mL 30 ISOLUTE 96 CN EC Fixed Well Plates Part number Description Quantity 421 0025 P01 ISOLUTE 96 CN EC 25 mg plate 1 ISOLUTE Array CN EC Loose Well
47. 0 positions or 6 mL with 5 positions Up to 10 units can be connected together and controlled through a simple easy to use software package See www biotage com applications for Application Note AN704 RapidTrace Procedure for the Automated Fractionation of Extractable Petroleum Hydrocarbons EPH in Soil using ISOLUTE EPH SPE Columns For ordering information see page 106 109 For up to date application notes please check www biotage com applications Environmental Applications and Products for Environmental Analysis Environmental Applications Application number Analyte Matrix Format Part number IST1102A IST1064 AN730 AN703 AN704 1511077 AN737 IST1025A AN701 IST1005A IST1046A AN716 Brominated Flame Retardants B Blockers Environmental Contaminants Including Dioxins and Furans Extractable Petroleum Hydrocarbons Extractable Petroleum Hydrocarbons Mineral Oil Organophosphate Pesticide Metabolites PAHs Pharmaceuticals TPH Total Oil and Grease Triazine Herbicides Triazines Plasma Biological fluids Soil and sediments Soil Soil VVater Urine VVater VVater VVater VVater VVater ISOLUTE ENV 200 mg 6 mL column ISOLUTE C2 100 mg 3 mL column RapidTrace and ISOLUTE NH2 500 mg 3 mL column ISOLUTE EPH 1 459 3 mL column RapidTrace and ISOLUTE EPH 1 45g 3 mL ISOLUTE Na zSO FL
48. 0 0010 A ISOLUTE MFC18 100 mg 1 mL 100 240 0010 B ISOLUTE MFC18 100 mg 3 mL 50 240 0010 G ISOLUTE MFC18 100 mg 10 mL 50 240 0020 B ISOLUTE MFC18 200 mg 3 mL 50 240 0020 H ISOLUTE MFC18 200 mg 10 mL 50 240 0050 B ISOLUTE MFC18 500 mg 3 mL 50 240 0050 C ISOLUTE MFC18 500 mg 6 mL 30 240 0050 H ISOLUTE MFC18 500 mg 10 mL 50 240 0100 C ISOLUTE MFC18 1 g 6 mL 30 240 0200 D ISOLUTE MFC18 2 g 15 mL 20 240 0500 E ISOLUTE MFC18 5 g 25 mL 20 ISOLUTE 96 MFC13 Fixed Well Plates Part number Description Quantity 240 0025 PO1 ISOLUTE 96 MFC18 25 mg plate 1 Support Documents TN101 Method Development in Solid Phase Extraction using Non polar ISOLUTE SPE Columns for the Extraction of Aqueous Samples TN112 General Approach to the Extraction of Basic Drugs from Biological Fluids using Non polar Non endcapped Sorbents See pages 156 and 157 for additional technical information about ISOLUTE MFC18 Applications Aqueous Wide polarity range Secondary Polar and weak cation exchange Chemical structure of C8 silane covalently bonded to the surface of a silica particle Ordering Information ISOLUTE C8 SPE Columns Part number Description Quantity 290 0002 A ISOLUTE C8 25 mg 1 mL 100 290 0005 A ISOLUTE C8 50 mg 1 mL 100 290 0010 A ISOLUTE C8 100 mg 1 mL 100 290 0010 B ISOLUTE C8 100 mg 3 mL 50 290 0020 B ISOLUTE C8 200 mg 3 mL 50 290 0020 H ISOLUTE C8 200 mg 10 mL 50 290 0050 B ISOLUTE C8 500 mg 3 mL 50 290 0050 C ISOLUTE C8 500 mg 6 mL 30 290 0
49. 0 121 0009 PTFE stopcock needle unit 10 121 0001 PTFE needle amp 10 121 0002 Stainless steel needle O 20 121 0003 Stainless steel needle retainer 10 121 0004 Port sealing plugs 30 121 0005 PTFE T valve 1 121 0010 VacMaster 10 silicone lid gasket 5 121 1030 VacMaster 10 complete lid 1 121 1045 VacMaster 10 complete tank 1 121 1039 VacMaster 20 silicone lid gasket 5 121 2059 VacMaster 20 complete lid 1 121 2075 VacMaster 20 complete tank 1 121 2068 VacMaster 96 Sample Processing Manifold The VacMaster 96 Sample Processing Manifold is a versatile system ideal for all 96 vvell SPE protein precipitation supported liquid extraction and filtra tion applications The small footprint and lightvveight construction make it suitable for manual processing or use vvith automated liquid handling systems Vacuum control accessories are separate from the manifold body allowing the use of a solvent trap see page 101 to prevent contamination of vacuum controls This is also convenient for use with liquid handling systems vvith built in vacuum control The manifold is designed for full compatibility vvith all commercially available extraction filtration and collection plates A simple system based on inserts and spacers optimizes extraction and collection plate positioning eliminating well to well contamination and sample loss The spacer required for compatibility with the modular Array format plate is supplied as standard with the VacMaste
50. 0 B 420 0050 C 420 0100 C 420 0100 P01 420 0100 T 421 0005 A 421 0010 A 421 0010 B 421 0020 B 421 0025 P01 421 0025 R 421 0050 B 421 0100 C 430 0010 A 430 0010 B 430 0010 G 430 0020 B 430 0050 B 430 0050 C 430 0050 H 430 0100 C 460 0002 A 460 0010 A 460 0010 B 460 0010 G 460 0020 B 460 0020 H 460 0050 B 460 0050 C 460 0050 H 460 0100 B 460 0100 C 460 0200 C 460 0200 D 460 0500 E 460 1000 F 470 0002 A 470 0005 A 470 0005 G 470 0010 A 470 0010 B 470 0010 G 470 0020 B 470 0025 R 470 0050 B 470 0050 C 470 0050 H 44 44 44 44 44 44 44 44 44 45 45 45 45 45 45 45 45 61 61 61 61 61 61 61 61 60 60 60 60 60 60 60 109 60 60 60 60 60 60 60 60 52 52 52 52 52 52 52 52 52 109 142 52 52 470 0050 P01 470 0100 C 470 0100 P01 470 0200 C 470 0200 D 480 0010 A 480 0010 B 480 0020 B 480 0025 P01 480 0025 R 480 0050 B 480 0050 C 480 0100 C 480 0100 P01 480 0100 T 500 0002 A 500 0005 A 500 0005 AG 500 0005 G 500 0010 A 500 0010 B 500 0010 C 500 0010 G 500 0020 B 500 0020 H 500 0025 P01 500 0050 B 500 0050 BG 500 0050 C 500 0050 H 500 0050 1 500 0050 R 500 0100 B 500 0100 C 500 0100 CG 500 0100 P01 500 0100 T 500 0200 D 503 0002 A 503 0005 A 503 0010 A 503 0010 B 503 0010 C 503 0020 B 503 0025 P01 503 0050 B 503 0050 C 503 0050 R 503 0100 C 503 0100 P01 503 0100 T 503 0200 D 520 0002 A 52 52 52 52 52 53 53 53 53 53 53
51. 01 25 2 125 129 See Application Note IST1069 for more information available at www biotage com applications See page 31 for ISOLUTE 101 SPE column ordering information ISOLUTE THC SPE Columns for the extraction of 11 Nor A THC 9 carboxylic acid from urine ISOLUTE THC columns are designed for the rapid cost effective extraction of the THC carboxylic acid metabolite from urine prior to confirmation analysis by GC MS The robust procedure based on a non polar retention mechanism has been shown to be most effective for THC metabolite confirmation testing Application Note IST1001 describes the extraction of the THC metabolite from urine Ordering Information Description Quantity Part number ISOLUTE THC 100 mg 1 mL 100 292 0010 A ISOLUTE THC 50 mg 10 mL 50 292 0005 G ISOLUTE THC 100 mg 3 mL 50 292 0010 B ISOLUTE HAX SPE columns Ideal for high purity extraction of acidic drugs from biological fluids ISOLUTE HAX SPE columns contain both non polar C8 and strong anion exchange NR3 functional groups Application Note IST1035 describes the use of the ISOLUTE Confirm HAX column for the extraction of the THC metabolite from urine Ordering information for ISOLUTE HAX SPE columns can be found on page 50 129 Forensic and Clinical Applications and Products for Forensic and Clinical Analysis Rapid Tracee SPE Workstation The Rapid Trace SPE Workstation is a modular highly scalable automated Sample
52. 1 189 98 98 98 98 70 71 174 184 185 103 106 109 97 98 87 54 54 147 57 57 SCX 2 Propylsulfonic acid 96 well SPE plates 58 Bulk sorbent 66 SPE columns 58 SCX 3 Ethylbenzene sulfonic acid 96 well SPE plates 59 SPE columns 29 SI Silica 96 well SPE plates 60 Bulk sorbent 66 SPE columns 60 Silica based sorbents see also individual chemistries SLE plates and columns 74 77 118 119 185 Sodium sulfate drying cartridge 89 150 Solid Phase Extraction Guide 174 184 Solvent evaporation 112 115 Solvent trap 99 Sorbent properties 154 155 174 184 Sorbent retention mechanisms 154 155 174 184 Sorbents Bulk ISOLUTE 66 67 SPE Dry 96 sample concentrator systems 112 Stainless steel frits 189 Stainless steel needles VacMaster 98 Stopcock needles PTFE 98 Stopcocks PTFE for universal use 98 Strong anion exchange see SAX PE AX Strong cation exchange see SCX 2 3 Supported Liquid Extraction SLE see HM N columns and SLE plates Styrene divinyl benzene SDVB see EVOLUTE ABN ENV and 101 T Tabless columns 26 30 32 46 54 94 96 122 188 Technical section 153 171 Technical support 6 Trap Kit VacMaster 96 VacMaster 99 THC SPE columns 129 TPH SPE columns 139 Triazine SPE columns 138 TurboVap 113 115 U Universal PTFE stopcock V VacMaster 10 20 VacMaster 96 VacMaster accessories VacMaster drying adapter VacMaster LVE kit V
53. 1 0025 R 36 1201 0125 H 87 121 5204 90 221 0025 P01 33 291 0050 36 1201 0126 D 87 121 5205 90 221 0025 R 33 291 0050 36 1201 0127 87 121 9600 99 221 0050 33 291 0050 PO1 36 1201 0128 F 87 121 9601 99 221 0050 C 33 291 0100 36 120 1021 A 88 121 9602 99 221 0050 H 33 291 0100 01 36 120 1022 88 121 9610 100 221 0050 P01 33 291 0200 D 36 120 1022 P01 90 121 9611 100 221 0050 R 33 291 0500 36 120 1023 88 121 9612 100 221 0100 33 292 0005 G 129 120 1024 88 121 9613 100 221 0100 33 292 0010 131 120 1025 D 88 121 9614 100 221 0100 CD 140 292 0010 B 129 131 120 1026 E 88 121 9615 100 221 0100 P01 33 320 0002 39 120 1028 F 88 123 2016 103 221 0100 T 33 320 0005 39 120 1031 A 88 123 2019 103 221 0200 C 33 320 0010 39 120 1032 G 88 124 1001 101 221 0200 D 33 320 0010 39 142 120 1033 B 88 124 2001 101 221 0500 E 33 320 0010 G 39 120 1034 H 88 220 0002 A 32 221 1000 F 33 320 0020 39 120 1035 C 88 220 0002 AG 32 96 222 0100 C 138 142 320 0025 PO1 39 120 1036 D 88 220 0005 A 32 240 0005 A 34 320 0025 R 39 120 1037 E 88 220 0005 G 32 240 0005 G 34 320 0050 B 39 120 1038 F 88 220 0010 A 32 240 0010 A 34 132 320 0050 39 120 1100 86 220 0010 B 32 240 0010 B 34 320 0050 01 39 120 1101 86 220 0010 BG 32 96 240 0010 G 34 132 320 0050 R 39 120 1102 86 220 0010 32 240 0020 34 320 0100 39 120 1103 86 220 0020 32 240 0020 34 320 0100 01 39 120 1151 148 220 0020 32 240 0025 01 34 320 0200 D
54. 10 C 30 986 0100 T 49 08 0002 19 137 SD 9600 DHS UK 112 915 0010 30 103186 115 142 147 151 SER PPM FYMP 95 915 0010 P01 30 103187 115 10 0002 18 127 5 1 95 915 0010 30 103188 115 147 151 SER PPM PL 95 915 0020 B 30 103189 115 10 0002 18 127 SER PPM SLVR 95 915 0020 B 151 103190 115 147 151 915 0020 C 30 142 103192 115 M18 0002 B 17 123 915 0020 CD 140 103193 115 127 132 137 915 0025 P01 30 103194 115 M18 0002 G 17 123 915 0025 R 30 103196 115 127 132 137 915 0050 B 30 103197 115 M21 0005 B 19 133 915 0050 BG 30 96 103198 115 M21 0005 G 19 135 915 0050 C 30 103199 115 M28 0002 B 17 127 915 0050 P01 30 103200 115 130 915 0100 C 30 151 103201 115 M34 0005 B 18 147 915 0100 CG 30 96 103202 115 151 915 0100 30 103203 115 M69 0002 B 19 147 9220 0100 66 103263 115 151 9221 0100 66 103264 115 72 0002 18 137 924 0100 151 125713 108 72 0002 18 137 927 0150 138 1412 133968 108 73 0002 18 147 928 0145 109 138 44139 115 151 142 44282 115 48 95 928 0500 138 44283 115 96 95 9290 0100 66 44577 115 PPM A48 1232 95 9291 0100 66 44880 115 48 1275 95 9320 0100 66 45269 115 48 13100 95 9321 0100 66 45286 115 48 16100 95 933 0050 137 47818 115 PPM A48 1RCK 95 933 0100 137 47820 115 PPM A48 3RCK 95 934 0040 137 48928 115 PPM A48 6RCK 95 935 0040 137 48929 11
55. 100 C ISOLUTE C6 1 g 6 mL ISOLUTE 96 C6 Fixed Well Plates Part number Description 380 0025 P01 ISOLUTE 96 C6 25 mg plate ISOLUTE Array C6 Loose Wells Part number Description 380 0025 R ISOLUTE Array C6 25 mg 1 mL wells Quantity 100 50 50 50 30 30 Quantity 1 Quantity 100 Chemical structure of C6 silane covalently bonded to the surface of a silica particle Pre assembled ISOLUTE Array plates are available To order add the suffix P to the equivalent loose well part number Support Documents TN101 Method Development in Solid Phase Extraction using Non polar ISOLUTE SPE Columns for the Extraction of Aqueous Samples TN112 General Approach to the Extraction of Basic Drugs from Biological Fluids using Non polar Non endcapped Sorbents See pages 156 and 157 for additional technical information about ISOLUTE C6 Applications Matrix Analytes 58 Retention Mechanisms Primary Non polar Aqueous Wide polarity range Secondary Polar and weak cation exchange Chemical structure of C4 silane covalently bonded to the surface of a silica particle Ordering Information ISOLUTE C4 SPE Columns Part number Description Quantity 390 0010 A ISOLUTE C4 100 mg 1 mL 100 390 0010 B ISOLUTE C4 100 mg 3 mL 50 390 0020 B ISOLUTE C4 200 mg 3 mL 50 390 0050 B ISOLUTE C4 500 mg 3 mL 50 390 0050 C ISOLUTE C4 500 mg 6 mL 30 390 0100 C ISOLUTE C4 1 g 6 mL 30 ISOLUTE 96 C4 Fixed Well Plates Part number Description Qua
56. 100 C ISOLUTE C8 1 g 6 mL 30 290 0200 D ISOLUTE C8 2 g 15 mL 20 ISOLUTE 96 C8 Fixed Well Plates Part number Description Quantity 290 0025 P01 ISOLUTE 96 C8 25 mg plate 1 290 0050 P01 ISOLUTE 96 C8 50 mg plate 1 290 0100 P01 ISOLUTE 96 C8 100 mg plate 1 ISOLUTE Array C8 Loose Wells Part number Description Quantity 290 0025 R ISOLUTE Array C8 25 mg 1 mL wells 100 290 0050 R ISOLUTE Array C8 50 mg 1 mL wells 100 290 0100 T ISOLUTE Array C8 100 mg 2 mL wells 100 Pre assembled ISOLUTE Array plates are available To order add the suffix P to the equivalent loose well part number Support Documents TN101 Method Development in Solid Phase Extraction using Non polar ISOLUTE SPE Columns for the Extraction of Aqueous Samples TN112 General Approach to the Extraction of Basic Drugs from Biological Fluids using Non polar Non endcapped Sorbents TN126 Method Development in Solid Phase Extraction using Non polar ISOLUTE SPE Columns for the Extraction of Biological Fluid Samples See pages 156 and 157 for additional technical information about ISOLUTE C8 pa A IARE ol fe r fa nd N b w 2 Applications Matrix Analytes Retention Mechanism 5 0 Aqueous Wide polarity range Primary Non polar S 0 SiCH Endcapped sorbent to minimize secondary silanol interactions Chemical structure of C8 silane and trimethyl silyl group covalently bonded to the surface of a silica particl
57. 128 ISOLUTE HCX 130 mg 3 mL column IST1002A Cocaine and Urine ISOLUTE HCX 902 0013 B 46 128 Benzoylecgonine 130 mg 3 mL column AN736 Cocaine VVhole blood TSOLUTE HCX 902 0013 H 46 128 Benzoylecgonine and and human 130 mg 10 mL column Cocaethylene tissue AN719 Cocaine and Biological EVOLUTE CX 601 0025 P01 25 metabolites fluids 25 mg fixed well plate 130 Applications and Products for Forensic and Clinical Analysis Forensic and Clinical Forensic Applications continued Application number AN718 AN748 IST1041A AN743 AN729 IST1081 AN741 IST1019A IST1039A IST1001A IST1015S IST1024A IST1035A AN720 AN731 IST1069A Analyte Ethyl Glucuronide EtG Ethyl Glucuronide EtG LSD Mephedrone Morphine and Codeine NIDA 5 SAMHSA 5 Opiates Phencyclidine Phencyclidine THC COOH THC COOH THC COOH THC COOH THC COOH Therapeutic and Illicit Drugs of Abuse Toxicology Screening Matrix Urine Urine Whole Blood Biological fluids Urine Urine Urine Meconium Urine Urine Urine Urine Urine Plasma Biological Fluids Postmortem Samples Format EVOLUTE AX 25 mg Array fixed vvell plate and other formats RapidTrace and EVOLUTE AX 100 mg 3 mL column ISOLUTE HCX 130 mg 10 mL column EVOLUTE CX 25 mg Array fixed vvell plate RapidTrace and ISOLUTE HCX 200 mg 3 mL column ISOLUTE HCX 130 mg 1 mL
58. 25 mg 1 mL Tabless ISOLUTE C18 50 mg 1 mL ISOLUTE C18 50 mg 10 mL ISOLUTE C18 100 mg 1 mL ISOLUTE C18 100 mg 3 mL ISOLUTE C18 100 mg 3 mL Tabless ISOLUTE C18 100 mg 10 mL ISOLUTE C18 200 mg 3 mL ISOLUTE C18 200 mg 10 mL ISOLUTE C18 500 mg 3 mL ISOLUTE C18 500 mg 3 mL Tabless ISOLUTE C18 500 mg 6 mL ISOLUTE C18 500 mg 10 mL ISOLUTE C18 1 g 3 mL ISOLUTE C18 1 g 6 mL ISOLUTE C18 1 g 6 mL Tabless ISOLUTE C18 2 g 6 mL ISOLUTE C18 2 g 15 mL ISOLUTE C18 5 g 25 mL ISOLUTE C18 10 g 70 mL ISOLUTE 96 Ci8 Fixed Well Plates Part number 220 0025 P01 220 0050 P01 220 0100 P01 Description ISOLUTE 96 C18 25 mg plate ISOLUTE 96 C18 50 mg plate ISOLUTE 96 C18 100 mg plate ISOLUTE Array C18 Loose Wells Part number 220 0025 R 220 0050 R 220 0100 T Description ISOLUTE Array C18 25 mg 1 mL wells ISOLUTE Array C18 50 mg 1 mL wells ISOLUTE Array C18 100 mg 2 mL wells Applications Matrix Analytes Retention Mechanisms Aqueous Quantity 100 100 100 50 100 50 50 50 50 50 50 50 30 50 50 30 30 30 20 20 16 Quantity 1 1 1 Quantity 100 100 100 VVide polarity range Primary Non polar Secondary Polar and vveak cation exchange Pre assembled ISOLUTE Array plates are available To order add the suffix P to the equivalent loose well part number Support Documents TN101 Method Development in Solid Phase Extraction using Non polar ISOLUTE SPE Columns for the Extraction of Aqueo
59. 25 mg 10 mL column ISOLUTE SLE 200 Supported Liquid Extraction plate ISOLUTE SLE 200 Supported Liquid Extraction plate ISOLUTE SLE 200 Supported Liquid Extraction plate ISOLUTE HCX 130 mg 3 mL column ISOLUTE SLE 200 Supported Liquid Extraction plate RapidTrace and ISOLUTE HCX 130 mg 3 mL column ISOLUTE SLE 200 Supported Liquid Extraction plate Part number 902 0013 H 601 0025 P01 M18 0002 B M18 0002 G 820 0200 PO1 820 0200 PO1 820 0200 PO1 902 0013 B 820 0200 PO1 902 0013 B 820 0200 PO1 Visit the Sample Preparation Application Database at www biotage com applications 46 128 25 17 127 137 17 127 137 76 76 76 46 128 76 46 128 76 Applications and Products for Forensic and Clinical Analysis Forensic and Clinical Applications and Products for Forensic and Clinical Analysis Biotage manufactures a vvide range of products for use in the area of forensic toxicology and doping control in animal and human sports EVOLUTE SPE Columns Developed specifically for analyte quantification by LC MS MS EVOLUTE products provide a highly effective solution to the problems of ion suppression and matrix effects from dirty extracts All EVOLUTE sorbents are now available in 50 um large format columns that allow for the larger volume and more viscous sample matrices encountered in forensic analysis EVOLUTE ABN is an advanc
60. 25 mg plate 1 532 0050 P01 ISOLUTE 96 SCX 2 50 mg plate 1 532 0100 P01 ISOLUTE 96 SCX 2 100 mg plate 1 ISOLUTE Array SCX 2 Loose Wells Part number Description Quantity 532 0025 R ISOLUTE Array SCX 2 25 mg 1 mL wells 100 Pre assembled ISOLUTE Array plates are available To order add the suffix P to the equivalent loose well part number Support Documents TN107 Method Development in Solid Phase Extraction using ISOLUTE SCX 2 SPE Columns for the Extraction of Aqueous Samples See pages 166 and 167 for additional technical information about ISOLUTE SCX 2 SOLUTE SCX 3 9 0 9 Applications Matrix Analytes Retention Mechanisms Primary Strong cation exchange Aqueous Cationic Secondary Non polar Primary Strong cation exchange Si OH A O Chemical structure of ethylbenzene sulfonic acid functional group covalently bonded to the surface of a silica particle Ordering Information ISOLUTE SCX 3 SPE Columns Part number Description Quantity 533 0002 A ISOLUTE SCX 3 25 mg 1 mL 100 533 0005 A ISOLUTE SCX 3 50 mg 1 mL 100 533 0010 A ISOLUTE SCX 3 100 mg 1 mL 100 533 0010 B ISOLUTE SCX 3 100 mg 3 mL 50 533 0020 B ISOLUTE SCX 3 200 mg 3 mL 50 533 0050 B ISOLUTE SCX 3 500 mg 3 mL 50 533 0050 C ISOLUTE SCX 3 500 mg 6 mL 30 533 0050 H ISOLUTE SCX 3 500 mg 10 mL 50 533 0100 B ISOLUTE SCX 3 1 g 3 mL 50 533 0100 C ISOLUTE SCX 3 1 g 6 mL 30 533 0200 D ISOLUTE SCX 3 2 g 15 mL 20 ISOLUTE 96 SCX 3 F
61. 4 g 6 mL column EVOLUTE WAX 25 mg Array fixed well plate ISOLUTE PAH 1 5 g 6 mL column EVOLUTE ABN 200 mg 6 mL column ISOLUTE O amp G 1 g 6 mL column 3 g 70 mL column ISOLUTE Triazine 1 g 6 mL column AFFINILUTE MIP Triazine 25 mg 10 mL column 915 0020 C 320 0010 B 470 0050 B 928 0145 B 928 0145 B 976 0400 C 604 0025 PO1 927 0150 C 610 0020 C 753 0100 CD 753 0300 FD 222 0100 C 08 0002 Visit the Sample Preparation Application Database at www biotage com applications 30 39 52 138 138 139 138 26 139 139 138 19 137 147 Environmental Applications and Products for Environmental Analysis T 8 1 i r m if VacMaster 10 shown using PTFE extraction caps Applications and Products for Agrochemical and Food Analysis Agrochemical and Food Applications and Products for Agrochemical and Food Analysis Sample preparation in food and agrochemical applications is often complicated by the nature of the original sample matrix In residue analysis common sample types such as fruit vegetables soil or tissue are solid or semi solid and need specific sample pre treatment methods to allovv extraction of analytes Some liquid samples such as milk have high fat concentrations making them difficult to handle using traditional methods In addition the complexity of these matrices requires addit
62. 46 533 0200 D 59 611 0010 H 26 714 0200 C 63 902 0030 46 128 600 0001 A 26 611 0020 26 714 0200 D 63 902 0030 CG 46 96 600 0002 A 26 611 0020 C 26 714 0500 E 63 902 0030 H 46 128 600 0002 AG 26 96 611 0050 C 26 714 1000 F 63 902 0050 P01 46 600 0002 B 26 611 0050 CG 26 96 715 0010 A 63 902 0050 R 46 600 0002 H 26 612 0005 26 27 715 0020 63 902 0100 01 46 600 0010 P01 25 109 132 715 0020 B 63 902 0100 46 600 0010 R 25 612 0010 26 715 0050 B 63 903 0005 50 600 0010 RP 25 612 0010 26 715 0050 63 903 0010 50 600 0025 01 25 612 0020 26 715 0100 B 63 903 0020 50 600 0025 25 612 0050 26 715 0100 63 903 0020 50 131 600 0025 RP 25 613 0005 26 715 0200 D 63 903 0025 01 50 601 0001 26 613 0010 26 109 715 0500 63 903 0025 50 601 0001 26 96 131 715 1000 F 63 903 0100 01 50 601 0002 26 613 0010 BG 26 96 752 0100 139 904 0010 51 601 0002 26 96 613 0010 26 753 0100 139 142 904 0030 51 149 601 0010 P01 25 613 0020 C 26 753 0300 FD 139 142 151 601 0010 R 25 613 0050 26 800 0040 79 904 0030 C 51 601 0010 RP 25 613 0050 26 96 800 0100 79 904 0050 B 51 601 0025 01 25 27 614 0005 26 800 0220 79 904 0100 C 51 151 123 130 131 614 0010 26 800 0350 79 905 0002 A 47 601 0025 25 614 0010 26 800 0700 FM 79 905 0005 47 601 0025 RP 25 614 0020 26 800 1300 FM 79 905 0010 47 602 0002 26 614 0050 26 802 0250
63. 5 48 5 95 9370 0100 148 48950 115 PPM A48 VVST 95 9371 0100 148 50000 108 96 1024 95 9460 0100 66 50974 108 96 5 95 9470 0100 66 50976 108 95 9480 0100 66 52006 108 Q0000 50V 71 9500 0100 66 52689 108 Q0010 15V 71 148 9503 0100 66 56536 108 Q0020 15V 71 148 9520 0100 66 56786 108 Q0030 15V 71 148 9532 0100 66 58309 108 Q0035 15V 71 148 9533 0100 66 59546 115 0Q0050 15V 71 148 9712 0100 66 61345 115 Q0060 15V 71 148 976 0400 139 142 M01 0002 G 17 147 Q0070 15V 71 148 9800 1000 67 151 000080 15 71 148 9800 5000 67 M02 0002 B 17 147 Q0090 15V 71 986 0002 A 49 151 SD2 9600 DHS EU 112 986 0005 A 49 M 2 0002 G 17 147 502 9600 5 112 986 0010 A 49 151 502 9600 5 112 986 0025 P01 49 M06 0002 B 19 127 SD2 9600 DHS UK 112 132 SD 9600 DHS EU 112 Limited VVarranty Biotage warrants that Biotage Consumables including all FLASH FLASH ISOLUTE EVOLUTE and Samplet purification cartridges scavenger resins solid bound reagents and microwave vials will be of good quality and workmanship and will meet the applicable product specification This warranty applies only to the initial test performed at the Customer s facility upon the initial start up of the consumables and expires at the time the user applies an actual sample to the consumable If the cartridge is packed vvith media provided by the Customer the Biotage vvar
64. 5 0020 B 08 0002 Visit the Sample Preparation Application Database at www biotage com applications 51 149 51 30 17 147 17 147 18 127 147 18 127 147 17 147 19 147 26 26 18 147 18 147 147 30 To 157147 Technical Section Product Selection Gulde Select a SPE Sorbent Neutral or hydrophobic EN O Acidic groups Aqueous Basic groups NHz NHt NH3t Organic Solvent Product Selection Gulde Non polar see pages 22 27 30 45 Adjust pH to neutralize analyte 2 pH units lt pK see pages 22 27 30 45 P E a Dual retention mechanism provides Mixed mode Non polar with Anion Exchange very clean extracts from biological see page 22 27 50 fluids Anion Exchange see pages 52 55 Non polar see pages 22 27 30 45 Mixed mode Non polar with Cation Exchange see pages 22 27 46 49 Cation Exchange see pages 56 59 Adjust pH to ionize analyte 2 pH units above pK If ionic strength matrix dilute or consider non polar or mixed mode SPE Adjust pH to neutralize analyte 2 pH units gt pK Dual retention mechanism provides very clean extracts from biological fluids Adjust pH to ionize analyte 2 pH units below pK If high ionic strength matrix dilute or consider non polar or mixed mode SPE Non Polar Sorbents Isolate analytes from aqueous or partiall
65. 84 QuickStart Guide for more information on utilizing secondary silanol interactions ISOLUTE Silica Based Non Polar Sorbents Description Butyl non endcapped C4 C2 Ethyl non endcapped PH Average Pore Particle Size Diameter Hm A 60 60 u 60 60 60 60 u m m HHH Cyanopropyl non endcapped Si CH gt 3CN 5 60 mn Ii 8 endcapped a chemical process to reduce the concentration of silica surface silanol groups that provide polar and vveak cation exchange secondary interactions 5 5 5 5 5 5 5 5 0 0 0 0 0 0 0 0 158 Non Polar Sorbents Application Chemistry Ordering Data Sheets Information Page Extraction from aqueous matrix using non polar interactions for acidic neutral and basic TN101 amp 112 compounds Secondary silanol or ionic interactions can be used to enhance extract purity and method robustness for basic compounds Most commonly used C2 sorbent for the extraction of acidic neutral and basic compounds TN101 1128 from aqueous matrices Secondary silanol or ionic interactions can be used to enhance extract purity and method robustness for basic compounds Extraction from aqueous matrix using non polar interactions for acidic neutral and basic compounds Extraction from aqueous matrix using non polar interactions for acidic neutral and basic compounds
66. A ISOLUTE Frit 6 mm 4 diameter 20 um PE G XL 100 120 1032 G ISOLUTE Frit 9 mm s diameter 20 um PE B 3 mL 100 120 1033 B ISOLUTE Frit 9 mm s diameter 20 um PE H XL 100 120 1034 H ISOLUTE Frit 13 mm 2 diameter 20 um PE C 6 mL 100 120 1035 C ISOLUTE Frit 16 mm s diameter 20 um PE D 15 mL 100 120 1036 D ISOLUTE Frit 20 mm 3 e diameter 20 um PE E 25 mL 100 120 1037 E ISOLUTE Frit 27 mm 2 6 diameter 20 um PE F 70 mL 100 120 1038 F The last character in the frit part number should match the last character of the reservoir part number into which it will be fitted ISOLUTE Filtration Columns Use as a stand alone filter or stack above an SPE column to increase the reservoir volume Fitted as standard with two 20 um polyethylene PE frits Ordering Information Description Quantity Part number ISOLUTE double fritted filtration column 1 mL 20 um PE 100 120 1021 A ISOLUTE double fritted filtration column 3 mL 20 um PE 100 120 1022 B ISOLUTE double fritted filtration column 6 mL 20 um PE 100 120 1023 C ISOLUTE double fritted filtration column 10 mL 20 um PE 50 120 1024 H ISOLUTE double fritted filtration column 15 mL 20 um PE 100 120 1025 D ISOLUTE double fritted filtration column 25 mL 20 um PE 100 120 1026 E ISOLUTE double fritted filtration column 70 mL 20 um PE 50 120 1028 F Accessories For SPE and Filtration ISOLUTE Sodium Sulfate Drying Cartridges
67. AX Loose Wells Part number Description Quantity 500 0050 R ISOLUTE Array SAX 50 mg 1 mL wells 100 500 0100 T ISOLUTE Array SAX 100 mg 2 mL wells 100 Pre assembled ISOLUTE Array plates are available To order add the suffix P to the equivalent loose well part number Support Documents TN103 Method Development in Solid Phase Extraction using ISOLUTE PE AX and SAX SPE Columns for the Extraction of Aqueous Samples See pages 164 and 165 for additional technical information about ISOLUTE SAX Tabless columns for use with Pressure Positive Pressure Manifolds and other automated SPE systems Other tabless columns are available contact Biotage for details Applications Matrix Analytes Retention Mechanisms Primary Strong anion exchange Aqueous Anionic Secondary Non polar Primary Strong anion exchange Ordering Information ISOLUTE PE AX SPE Columns Part number Description Quantity 503 0002 A ISOLUTE PE AX 25 mg 1 mL 100 503 0005 A ISOLUTE PE AX 50 mg 1 mL 100 503 0010 A ISOLUTE PE AX 100 mg 1 mL 100 503 0010 B ISOLUTE PE AX 100 mg 3 mL 50 503 0010 C ISOLUTE PE AX 100 mg 6 mL 30 503 0020 B ISOLUTE PE AX 200 mg 3 mL 50 503 0050 B ISOLUTE PE AX 500 mg 3 mL 50 503 0050 C ISOLUTE PE AX 500 mg 6 mL 30 503 0100 C ISOLUTE PE AX 1 g 6 mL 30 503 0200 D ISOLUTE PE AX 2 g 15 mL 20 ISOLUTE 96 PE AX Fixed Well Plates Part number Description Quantity 503 0025 P01 ISOLUTE 96 PE AX 25 mg plate 1 503 0100 PO1 ISOLUTE 96 PE AX 100 m
68. B AFFINILUTE MIP NNAL 25 mg 10 mL 50 M06 0002 G AFFINILUTE MIP NNAL 25 mg 3 mL 50 M06 0002 B AFFINILUTE MIP TSNAs 50 mg 10 mL 50 M10 0002 G AFFINILUTE MIP TSNAs 50 mg 3 mL 50 M10 0002 B Products for Doping Control Applications ISOLUTE C8 C18 and ENV columns are ideal for applications in doping control in sport such as the extraction of steroids and their glucuronide and sulphate metabolites from human urine For example ISOLUTE C8 is used to extract 5 a dihydrotestosterone from athlete s urine ref Kicman et al Clinical Chemistry 1995 41 11 1617 1627 For ISOLUTE non polar sorbents ordering information see pages 30 45 156 159 ISOLUTE HCX Columns for High Extract Purity Extract basic drugs from biological fluids with ISOLUTE HCX mixed mode SPE columns that provide two primary retention mechanisms non polar C8 and strong cation exchange This dual retention mechanism allows a rigorous interference elution regime to be used without reducing recovery of the analytes of interest compared with non selective LLE resin or C18 based methodologies ISOLUTE HCX columns provide extremely high purity extracts of drugs of abuse from biological fluids such as urine plasma and whole blood see Figure 1 Biotage s range of reservoirs and sorbent bed mass options ensure automation compatibility and capacity requirement of Figure 1 Chromatograrni ae I DIE ede yee diverse sample matrices such as hydrolyzed hair equine u
69. Biotage Analytical Sample Preparation 2012 Catalog Applications e Agrochemical amp Food e Bioanalysis e Clinical e Environmental e Forensic Consumables e ISOLUTE SLE e EVOLUTE Polymeric SPE e ISOLUTE SPE Columns and 96 Well Plates e AFFINILUTE MIP e ISOLUTE QuEChERS e ISOLUTE SPE Columns and 96 Well Plates Instruments e PRESSURE Manifolds e RapidTrace SPE Automation e SPE Dry Evaporators TurboVap Evaporators New Analytical Instruments O Biotage cD biotage com sampleprep pg 94 Biotage CD biotage com sampleprep 2012 Analytical Sample Preparation Catalog Nevv Products 1 Point Support Sample Prep Format Options AFFINILUTE MIP EVOLUTE SPE Columns and 96 well Plates ISOLUTE SPE Columns and 96 well Plates ISOLUTE Bulk SPE Sorbents ISOLUTE QuEChERS Supported Liquid Extraction Products ISOLUTE SLE ISOLUTE HM N Protein Precipitation Products Sample Preparation Accessories Manual Sample Processing Products PRESSURE 48 and PRESSURE 96 VacMaster 10 and 20 VacMaster 96 Sample Processing Accessories Gravity Rack Automated Sample Processing Products RapidTrace Evaporation SPE Dry 96 and 96 Dual TurboVap Applications and Products for Bioanalysis Applications and Products for Forensic and Clinical Analysis Applications and Products for Environmental Analysis Applications and Products for Agrochemical and F
70. C ISOLUTE C18 EC 1 g 6 mL 30 221 0200 C ISOLUTE C18 EC 2 g 6 mL 30 221 0200 D ISOLUTE C18 EC 2 g 15 mL 20 221 0500 E ISOLUTE C18 EC 5 g 25 mL 20 221 1000 F ISOLUTE C18 EC 10 g 70 mL 16 ISOLUTE 96 C18 EC Fixed Well Plates Part number Description Quantity 221 0025 PO1 ISOLUTE 96 C18 EC 25 mg plate 1 221 0050 PO1 ISOLUTE 96 C18 EC 50 mg plate 1 221 0100 P01 ISOLUTE 96 C18 EC 100 mg plate 1 ISOLUTE Array C18 EC Loose Wells Part number Description Quantity 221 0025 R ISOLUTE Array C18 EC 25 mg 1 mL wells 100 221 0050 R ISOLUTE Array C18 EC 50 mg 1 mL wells 100 221 0100 T ISOLUTE Array C18 EC 100 mg 2 mL vvells 100 Pre assembled TSOLUTE Array plates are available To order add the suffix P to the equivalent loose vvell part number Support Documents TN101 Method Development in Solid Phase Extraction using Non polar ISOLUTE SPE Columns for the Extraction of Aqueous Samples See pages 156 and 157 for additional technical information about ISOLUTE C18 EC Applications Matrix Analytes 58 Retention Mechanisms Primary Non polar VVide polarity range pa Si 0 5 CH CH Aqueous CH Secondary Polar and weak cation exchange SI OH Chemical structure of monofunctional C18 silane covalently bonded to the surface of a Silica particle Ordering Information ISOLUTE MFC18 SPE Columns Part number Description Quantity 240 0005 A ISOLUTE MFC18 50 mg 1 mL 100 240 0005 G ISOLUTE MFC18 50 mg 10 mL 50 24
71. Columns Part number Description Quantity 915 0001 A ISOLUTE ENV 10 mg 1 mL 100 915 0002 A ISOLUTE ENV 25 mg 1 mL 100 915 0002 G ISOLUTE ENV 25 mg 10 mL 50 915 0005 A ISOLUTE ENV 50 mg 1 mL 100 915 0005 AG ISOLUTE ENV 50 mg 1 mL Tabless 100 915 0005 B ISOLUTE ENV 50 mg 3 mL 50 915 0005 G ISOLUTE ENV 50 mg 10 mL 50 915 0010 A ISOLUTE ENV 100 mg 1 mL 100 915 0010 B ISOLUTE ENV 100 mg 3 mL 50 915 0010 BG ISOLUTE ENV 100 mg 3 mL Tabless 50 915 0010 C ISOLUTE ENV 100 mg 6 mL 30 915 0010 H ISOLUTE ENV 100 mg 10 mL 50 915 0020 B ISOLUTE ENV 200 mg 3 mL 50 915 0020 C ISOLUTE ENV 200 mg 6 mL 30 915 0050 B ISOLUTE ENV 500 mg 3 mL 50 915 0050 BG ISOLUTE ENV 500 mg 3 mL Tabless 50 915 0050 C ISOLUTE ENV 500 mg 6 mL 30 915 0100 C ISOLUTE ENV 1 g 6 mL 30 915 0100 CG ISOLUTE ENV 1 g 6 mL Tabless 30 915 0100 E ISOLUTE ENV 1 g 15 mL 20 ISOLUTE 96 ENV Fixed Well Plates Part number Description Quantity 915 0010 PO1 ISOLUTE 96 ENV 10 mg plate 1 915 0025 P01 ISOLUTE 96 ENV 25 mg plate 1 915 0050 P01 ISOLUTE 96 ENV 50 mg plate 1 ISOLUTE Array ENV Loose Wells Part number Description Quantity 915 0010 R ISOLUTE Array ENV 10 mg 1 mL wells 100 915 0025 R ISOLUTE Array ENV 25 mg 1 mL wells 100 Pre assembled ISOLUTE Array plates are available To order add the suffix P to the equivalent loose well part number Support Documents TN109 Method Development in Solid Phase Extraction using ISOLUTE ENV SPE Columns for the Extract
72. Description Quantity Part number Columns for automated method 50 928 0145 B ISOLUTE EPH 1 45 g 3 mL Columns for manual method 20 928 0500 E ISOLUTE EPH M 5 g 25 mL ISOLUTE PAH SPE Columns ISOLUTE PAH SPE columns are designed to extract PAHs from water samples containing polar interferences such as humic acids The layered column contains a NH2 layer above a C18 sorbent bed Polar interferences are retained on the NH2 layer during extraction but are not eluted with the PAHs giving a pure extract for analysis Request Application Note IST1025 for more information Ordering Information Description Quantity Part number ISOLUTE PAH 1 5 g 6 mL 30 927 0150 C ISOLUTE Triazine SPE Columns ISOLUTE Triazine SPE columns are optimized for reliable reproducible extraction of triazine pesticides and their polar metabolites from water samples Each batch of ISOLUTE Triazine is quality controlled for recoveries of the atrazine desethyl and desisopropyl metabolites Request Application Note IST1046 for more information Ordering Information Description Quantity Part number ISOLUTE Triazine 1g 6 mL 30 222 0100 C Environmental Applications and Products for Environmental Analysis ISOLUTE TPH SPE Columns Extract total petroleum hydrocarbons from water samples with ISOLUTE TPH SPE columns The simple method eliminates tedious sample shaking and emulsion formation problems associated with liquid liquid extraction Manual and au
73. E Bulk Sorbents The same high quality sorbents used to manufacture ISOLUTE SPE columns and 96 vvell plates are also available in bulk The tightly controlled physical and chemical properties of these sorbents which provide reproducible performance in SPE procedures also ensure their suitability when used in applications that require loose material Ordering Information Non polar Sorbents Part number Description Quantity 9220 0100 ISOLUTE C18 bulk 100 g 9221 0100 ISOLUTE C18 EC bulk 100 g 9290 0100 ISOLUTE C8 bulk 100 g 9291 0100 ISOLUTE C8 EC 4 bulk 100 g 9320 0100 ISOLUTE C2 bulk 100 g 9321 0100 ISOLUTE C2 EC 4 bulk 100 g A EC endcapped a chemical process to reduce the concentration of silica surface silanol groups that provide polar and weak cation exchange secondary interactions For more details see the QuickGuide to SPE pages 173 185 Polar Sorbents Part number Description Quantity 9460 0100 ISOLUTE SI bulk 100 g 9470 0100 ISOLUTE NH2 bulk 100 g 9480 0100 ISOLUTE PSA bulk 100 g 9712 0100 ISOLUTE FL bulk 100 g Ion Exchange Sorbents Part number Description Quantity 9470 0100 ISOLUTE NH2 bulk 100 g 9480 0100 ISOLUTE PSA bulk 100 g 9500 0100 ISOLUTE SAX bulk 100 g 9503 0100 ISOLUTE PE AX bulk 100 g 9520 0100 ISOLUTE CBA bulk 100 g 9532 0100 ISOLUTE SCX 2 bulk 100 g 9533 0100 ISOLUTE SCX 3 bulk 100 g ISOLUTE sorbents are available in additional quantities to those detailed in the above table P
74. G ISOLUTE HCX 25 mg 1 mL Tabless 100 902 0013 BG ISOLUTE HCX 130 mg 3 mL Tabless 50 902 0013 CG ISOLUTE HCX 130 mg 6 mL Tabless 30 902 0030 CG ISOLUTE HCX 300 mg 6 mL Tabless 30 ISOLUTE ENV 915 0005 AG ISOLUTE ENV 50 mg 1 mL Tabless 100 915 0010 BG ISOLUTE ENV 100 mg 3 mL Tabless 50 915 0050 BG ISOLUTE ENV 500 mg 3 mL Tabless 50 915 0100 CG ISOLUTE ENV 1 g 6 mL Tabless 30 Other configurations are available at the same cost as the standard column format VacMaster 10 amp 20 Sample Processing Manifolds VacMaster Sample Processing Manifolds are used to process up to ten VacMaster 10 or twenty VacMaster 20 samples simultaneously Designed to meet the most demanding criteria for safety extract purity flexibility and ease of use VacMaster manifolds provide productivity enhancements in many applications Both VacMaster 10 and 20 Ii manifolds are supplied complete vvith thick vvalled LU glass tank PTFE lid fine vacuum control and vacu um safety valve inert polyethylene collection tube rack port sealing plugs and replaceable stainless steel needles that are compatible vvith all industry standard Luer tipped SPE columns Features and Advantages e Robust inert system constructed from glass PTFE and polyethylene avoids sample contamination e Optional PTFE stopcock and stopcock needles allow for additional flow control and purity e Multiple stations allow for parallel processing of S
75. H and temperature allowing for stringent elution interference vvashes and extended shelf life AFFINILUTE MIP Amphetamines One of the difficulties of analyzing amphetamines is the short half life of the drug once ingested such that testing by conventional methods needs to occur within 48 hours of ingestion The class selectivity of AFFINILUTE MIP Amphetamines allovvs for amphetamine detection up to 5 days after intake Additionally the class selectivity vvill allovv for the capture of designer drugs based on the amphetamine core structure Description Quantity Part number AFFINILUTE MIP Amphetamines 25 mg 3 mL 50 M28 0002 B AFFINILUTE MIP B agonists B 2 adrenergic receptor agonists B agonists have been internationally banned in human and equine sports in addition to being prohibited for non veterinary use in livestock After therapeutic use these drugs are not completely metabolized and are often excreted in wastewaters As a result there is concern for the long term chronic effects of these drugs on humans and the ecosystem Description Quantity Part number AFFINILUTE MIP Full B agonist 25 mg 10 mL 50 MO2 0002 G AFFINILUTE MIP Full B agonist 25 mg 3 mL 50 MO2 0002 B AFFINILUTE MIP f blockers B adrenergic blocking agents B blockers are a class of drugs used to treat various cardiac disorders such as hypertension angina congestive heart failure and arrhythmia B blockers have been used to enhance performance by athletes by lowe
76. L to 5 mL sample volumes A generic procedure for supported liquid extraction of biological fluids using the ISOLUTE SLE plate is included with every plate Step 1 Step Step 3 Apply Aqueous Wait 5 minutes Add Organic Sample Solvent Whole sample is absorbed onto dry column Unlike SPE no flow through Collect organic extract containing drug Figure 1 Schematic of Supported Liquid Extraction Process 74 Supported Liquid Extraction Efficient Extraction Supported liquid extraction mechanisms are very efficient delivering higher analyte recoveries and cleaner extracts than the equivalent LLE methods No Emulsion Formation Emulsions cannot form because the sample and immiscible extraction solvent are never in direct contact preventing contamination and maximizing analyte recovery Easy to Automate ISOLUTE SLE plates provide an easy to automate alternative to traditional liquid liquid extraction No manual off line steps capping mixing centrifuge de capping are required All procedural steps can be fully automat ed with no manual intervention necessary Higher Productivity Using ISOLUTE SLE plates 96 samples can be prepared in approximately half the time needed for liquid liquid extraction as shown in Table 1 Time for processing 96 samples Table 1 Using the Quadra 96 liquid handling system standard ISOLUTE SLE generic procedure vs equivalent LLE procedure Good Flow Characteris
77. Matrix Urine Plasma Plasma Plasma VVhole blood Urine Plasma Plasma Plasma Format ISOLUTE SLE 200 Supported Liquid Extraction plate ISOLUTE SLE 200 Supported Liquid Extraction plate ISOLUTE SLE 200 Supported Liquid Extraction plate ISOLUTE SLE 200 Supported Liquid Extraction plate RapidTrace and ISOLUTE HCX 130 mg 3 mL column AFFINILUTE MIP 50 mg 3 mL or 50 mg 10 mL column RapidTrace and ISOLUTE C18 EC 200 mg 3 mL column ISOLUTE SLE 200 Supported Liquid Extraction plate ISOLUTE SLE 200 Supported Liquid Extraction plate Part number 820 0200 P01 820 0200 P01 820 0200 PO1 820 0200 1 902 0013 21 0005 M21 0005 G 221 0020 B 820 0200 P01 820 0200 P01 Visit the Sample Preparation Application Database at www biotage com applications 76 76 76 76 46 128 19 19 33 76 76 133 Applications and Products for Environmental Analysis 136 Environmental Applications and Products for Environmental Analysis Extract purity is essential for reliable analyte quantification at trace levels for environmental applications These very demanding requirements are met by ISOLUTE and EVOLUTE SPE column components sorbents columns and frits and VacMaster Sample Processing Manifolds All column components are cleaned to a high standard and quality controlled for purity using both GC and LC techniques VacMa
78. N consists of a modified polystyrene divinylbenzene polymer for reversed phase non polar or hydrophobic retention incorporating non ionizable hydroxyl groups which ensure the phase is fully water wettable Non polar hydrophobic groups 22 EVOLUTE Advanced Polymeric Sorbents for LC MS MS Applications EVOLUTE Strong Strong VVeak cation VVeak anion Functionality Non polar cation anion Sorbent exchange exchange exchange exchange EVOLUTE ABN Non polar Mixed mode sorbents EVOLUTE CX up and S037 Non polar and EVOLUTE WCX Non polar and EVOLUTE AX N CH3 3 Non polar and EVOLUTE WAX NHS NH gt Table 1 Summary of EVOLUTE family functionality primary interaction secondary interaction EVOLUTE Extract purity EVOLUTE SPE sorbents are manufactured with a very narrow pore size distribution see Figure 2 Pore size optimization dramatically reduces protein contamination in extracts of biological fluids as illustrated see Figure 3 Such removal or reduction of protein contamination from sample extracts avoids their transfer into the analytical system thus reducing matrix effects in LC MS analysis Additionally increased back pressure in UPLC systems due to protein contamination is eliminated avoiding the need for column back flushing and or frequent replacement of guard columns VOLUTE o 3 Competitor O Mwt Marker e Raw Serum J r
79. Oxprenolol M Propranolol Ordering Information Description Fixed Well Plates ISOLUTE SLE 200 HL Supported Liquid Extraction Plate ISOLUTE SLE 400 HL Supported Liquid Extraction Plate Array Wells ISOLUTE SLE 200 uL Array Wells ISOLUTE SLE 400 uL Array Wells Columns ISOLUTE SLE 1 mL Sample Volume ISOLUTE SLE 2 mL Sample Volume ISOLUTE SLE 5 mL Sample Volume ISOLUTE SLE 10 mL Sample Volume 2 mLSample 2x5mL Elution Elution Elution Elution 5 mL Sample 2x10 mL Elution M Alprenolol 2 mLSample 2x5mL Elution Elution Elution Elution 5 mL Sample 2x10 mL Elution M Alprenolol Quantity 100 100 30 20 20 16 Figure 3 Scalability of columns using both NSATDS A and B Blocker B analyte suites Part number 820 0200 P01 820 0400 P01 820 0200 T 820 0400 T 820 0140 C 820 0290 D 820 0690 E 820 1420 F Supported Liquid Extraction Applications Application number AN742 AN746 AN751 AN602 AN603 AN741 AN721 AN722 AN740 AN720 AN601 AN735 AN734 Visit the Sample Preparation Application Database at www biotage com applications Analyte Amphetamines Amphetamines Benzodiazepines Corticosteroids Non steroidal Anti inflammatory Drugs NSAIDs Opiates Tamoxifen Tamoxifen Testosterone and other steroid hormones THC COOH Tricyclic Antidepressants Vitamin D and metabolites Warfarin Matrix Urine U
80. PE and filtration applications e Thick walled glass tank allows clear view of the sample processing operation e Collection tube rack with easy lift carrying handle allows for safe transfer of samples e Vacuum safety valve fitted as standard to ensure safe sample processing e Range of accessories for enhancing productivity Versatile Collection Rack Resistant to commonly used organic solvents acids and bases the VacMaster collection rack is constructed from inert material Spacing between each rack plate is fully adjustable to fit a wide range of collection vessels The carrying handle makes lifting and transporting fully loaded racks easy and safe Racks with alternative diame ter holes are available through our Custom Manufacturing Service see page 187 189 Stopcock Options We recommend VacMaster 10 or 20 manifolds are fitted with PTFE stopcocks for optimum flow control For a totally inert system the standard stainless steel needles can be replaced with optional one piece PTFE needles or stopcock needle units see page 98 Both have excellent solvent resistance and smooth operation without sticking Safety Features Biohazard safety for biological matrices such as plasma or urine unique design features and operating pro cedures provide a safer working system With the lid mounted vacuum controls it is possible to use one tank to process samples and dedicate a second tank for analyte elution only avoiding any contamination Both ta
81. Prep platform designed for high throughput Units are available to accommodate 1 mL 3 mL and 6 mL industry standard SPE cartridges and can process up to 100 samples in less than tvvo hours The RapidTrace excels in automating low volume solid phase extractions and has proven to be highly effective in high throughput forensic laboratories for the extraction of drugs of abuse from various biological matrices including urine and oral fluid Analysis of samples can then be effectively performed through either LC MS MS or GC MS For more details of RapidTrace application for the forensic and clinical market please refer to the sample prep data base on www biotage com applications Forensic Applications let Analyte Matrix Format Part number number IST1044A Acidic Neutral and Urine ISOLUTE HCX 902 0013 H 46 128 Basic Drugs 130 mg 10 mL column IST1034A Amphetamine and Urine ISOLUTE HCX 902 0013 B 46 128 Methamphetamine 130 mg 3 mL column AN708 Amphetamines Urine AFFINILUTE MIP M28 0002 B 17 127 25 mg 3 mL column AN742 Amphetamines Urine ISOLUTE SLE 200 820 0200 PO1 76 Supported Liquid Extraction plate AN746 Amphetamines Urine ISOLUTE SLE 820 0140 C 76 1 mL column IST1032A Barbiturates Urine ISOLUTE HCX 902 0013 B 46 128 130 mg 3 mL column AN727 Basic Drugs of Abuse Biological RapidTrace and 902 0020 B 46 fluids ISOLUTE HCX 200 mg 3 mL column AN725 Benzoylecgonine Urine RapidTrace and 902 0013 B 46
82. SA also retain analytes using hydrogen bonding However the analyte is not so strongly retained on these sorbents and maybe eluted using less polar solvents ISOLUTE CN typically retains analytes using dipole dipole interactions ISOLUTE Silica Based Polar Sorbents Description Average Pore Particle Size Diameter A Cyanopropyl non endcapped Si CH zCN 60 u 2 3 dihydroxypropoxypropyl non end capped Si CH 3 OCH CH CH OH OH Aminopropyl non endcapped Si CH gt 3NH gt Ethylenediamine N propyl non end Si CH gt 3NH CH gt 2NH gt capped Polar Sorbents Nominal Application Chemistry Ordering Moisture Data Information Content Sheets Extraction of polar compounds from a non polar sample matrix Retains analytes using dipole dipole interactions Can also be used as a non polar sorbent with aqueous sample matrices Extraction of polar compounds from a non polar sample matrix using hydrogen bonding retention mechanism Extraction of polar compounds from a non polar sample matrix using hydrogen bonding retention mechanism Less retentive sorbent than SI Can also be used as a non polar sorbent with aqueous sample matrices Extraction of polar compounds from a non polar sample matrix using hydrogen bonding retention mechanism Less retentive sorbent than SI Can also be used as a weak anion exchange sorbent in aqueous and non polar sample matrices Extraction of polar compounds
83. Sample Preparation 79 SOLUT No 4 rt OP 1A nn Protein Precipitation Products ISOLUTE PPT ISOLUTE PPT Protein Precipitation Plates Protein precipitation is a routinely used sample preparation technique for removal of proteins from biological fluid samples prior to analysis Protein precipitation by filtration in the 96 well format is a high throughput alternative to the traditional centrifugation based technique using collection plates or micro tubes ISOLUTE PPT Protein Precipitation Plates have revolutionized this sample preparation technique The optimized filtration system in ISOLUTE PPT plates provides an easy to automate solution for efficient protein removal The procedure is streamlined with no off line steps The proprietary filtration system allows for solvent first methodology such that the plasma sample can be applied to a pool of solvent typically acetonitrile This results in very efficient protein precipitation Unlike membrane based devices the system has an optimized porosity distribution and acts as a depth filter holding up the precipitated protein without well blockage whilst allowing rapid transfer of the filtrate to the collection plate see Figure 1 Features and Advantages e Solvent first methodology produces efficient protein removal eliminating cloudy extracts e Optimized filter bed prevents well blocking e No need for off line
84. Sample Preparation Application Database is a vital resource for analytical chemists looking to optimize sample preparation procedures As sample preparation experts Biotage are dedicated to finding the ideal solution to your sample preparation requirements vvith a regularly updated database containing application notes technical notes and scientific presentations The Sample Preparation Application Database has been optimized to provide the most appropriate efficient and effective sample preparation solutions taking into consideration matrix effects analyte structure functionality and relevant regulatory requirements The Sample Preparation Application Database is fully searchable by keyword analyte matrix analytical technique format product type and industry type This search feature allows analytical chemists to obtain relevant application notes suitable for their needs in an efficient and dynamic manner If you cannot find a suitable application on the database the Sample Preparation Application Database can direct an email request to one of our fully trained application specialists who will endeavour to find a suitable solution Visit the Sample Preparation Application Database www biotage com applications Contact 1 Point Support Contact Biotage 1 Point Support VVVVVV biotage com The Biotage vveb site offers our customers easy access to current information on nevv products applications and events Europe Service and Support Te
85. The use of sodium sulfate Na5SO for drying water immiscible sample extracts prior to analyte derivatization or analysis is well documented Traditionally this is achieved by adding loose material to the extract followed by filtering or pipetting off the dry solvent ISOLUTE Sodium Sulfate Drying Cartridges are designed for efficient in line drying of water immiscible extraction solvents The cartridges feature a female Luer inlet and male Luer outlet allowing them to be attached to the outlet of any SPE column Elution solvents are dried as they pass through the cartridge The cartridges contain 2 5 g of high purity sodium sulfate with capacity for up to 1 2 mL of water per gram For additional capacity two or more cartridges can be joined in series ISOLUTE sodium Solvent blank sulfate drying internal cartridges Standard Competitor A ol Competitor B an 1 GC chromatograms comparing the purity of ISOLUTE sodium sulphate drying cartridges with equivalent competitor products Ordering Information Description Quantity Part number ISOLUTE Sodium Sulfate Drying Cartridge 50 802 0250 M 89 90 Accessorles For SPE and Filtration ISOLUTE Filtration Plates for High Throughput Applications Suitable for general filtration applications ISOLUTE filtration plates are fitted with a single 20 um polyethylene PE frit Ordering Information Description Quantity Part number Filtration pl
86. UTE HAX 200 mg 3 mL 50 903 0020 H ISOLUTE HAX 200 mg 10 mL 50 ISOLUTE 96 HAX Fixed Well Plates Part number Description Quantity 903 0025 P01 ISOLUTE 96 HAX 25 mg plate 1 903 0100 P01 ISOLUTE 96 HAX 100 mg plate 1 ISOLUTE Array HAX Loose Wells Part number Description Quantity 903 0025 R ISOLUTE Array HAX 25 mg 1 mL wells 100 Pre assembled ISOLUTE Array plates are available To order add the suffix P to the equivalent loose well part number Support Documents TN127 Method Development in Solid Phase Extraction using ISOLUTE HAX for the Extraction of Drugs from Biological Fluid Samples See pages 162 and 163 for additional technical information about ISOLUTE HAX SOLUTE MULTIMODE Octadecyl Quaternary Amine and Sulfonic Acid Applications Matrix Analytes Retention Mechanisms Si O i CH N CH C Non polar Aqueous anionic and cationic Non polar polar anion and cation exchange Si O Si CH CH Si O Si R SO H ISOLUTE Multimode combines C18 Octadecyl quaternary amine and sulfonic acid functionalities Ordering Information ISOLUTE Multimode SPE Columns Part number Description Quantity 904 0010 A ISOLUTE Multimode 100 mg 1 mL 100 904 0030 B ISOLUTE Multimode 300 mg 3 mL 50 904 0030 C ISOLUTE Multimode 300 mg 6 mL 30 904 0050 B ISOLUTE Multimode 500 mg 3 mL 50 904 0100 C ISOLUTE Multimode 1 g 6 mL 30 Support Documents IST1022 Extraction of
87. acMaster sample processing manifolds Vacuum control units VCU 1 VCU 2 with integral vacuum generator Vacuum manifold 98 97 98 99 100 101 102 101 102 140 97 100 99 99 see VacMaster and VacMaster 96 W Weak anion exchange see NH2 PSA and EVOLUTE Weak cation exchange see CBA XL SPE columns and EVOLUTE Part Number Index Part Number Page 121 0003 98 103 220 0100 B 32 290 0010 B 35 101 0010 B 31 121 0004 98 103 220 0100 C 32 290 0020 H 35 101 0020 B 31 121 0005 98 220 0100 CD 140 290 0025 P01 35 101 0020 C 31 121 0009 98 103 220 0100 CG 32 96 290 0025 R 35 101 0050 B 31 121 0010 98 220 0100 P01 32 290 0050 B 35 101 0050 C 31 121 1016 98 220 0100 32 290 0050 C 35 120 1000 P01 83 91 121 1030 98 220 0200 C 32 290 0050 01 35 120 1001 87 121 1039 98 220 0200 D 32 290 0050 35 120 1002 87 121 1045 98 220 0500 32 290 0100 35 120 1003 87 121 2016 98 220 1000 32 290 0100 01 35 120 1003 86 121 2059 98 221 0002 33 290 0100 35 120 1006 87 121 2068 98 221 0005 33 290 0200 D 35 120 1007 E 87 121 2075 98 221 0005 G 33 291 0010 36 120 1009 F 87 121 2085 141 221 0010 33 291 0010 B 36 120 1009 FD 86 121 2086 141 221 0010 B 33 291 0010 G 36 1201 0120 87 121 2090 102 221 0010 G 33 291 0020 B 36 1201 0121 A 87 121 2095 101 221 0020 B 33 109 291 0020 H 36 1201 0122 B 87 121 5202 91 95 133 291 0025 01 36 1201 0123 C 87 121 5203 91 95 221 0020 H 33 29
88. al and Food Analysis Food Applications Application Analyte Matrix Format Part number number IST1076A IST1082 AN706 AN711 AN709 AN714 AN732 AN749 AN710 AN715 IST1027A IST1097 AN716 Acrylamide Acrylamide B Agonists Chloramphenicol Clenbuterol Fluoroquinolones Melamine Melamine Nitroimidazoles PAHs Pesticides Pesticides Triazines Cooked Foodstuffs Foodstuffs Biological Matrices Biological Matrices Urine Biological Matrices Milk Milk Biological Matrices Olive Oil Vegetables Vegetables and Fruits Water ISOLUTE Multimode 300 mg 3 mL column ISOLUTE Multimode 1 9 3 mL and ISOLUTE ENV 1 9 6 mL column AFFINILUTE MIP B agonists 25 mg 3 mL or 25 mg 10 mL column AFFINILUTE MIP Chloramphenicol 25 mg 3 mL or 25 mg 10 mL column AFFINILUTE MIP Clenbuterol 25 mg 10 mL column AFFINILUTE MIP Fluoroquinolones 25 mg 3 mL column EVOLUTE CX 50 mg 3 mL column RapidTrace and EVOLUTE CX 100 mg 3 mL column AFFINILUTE MIP Nitroimidazoles 50 mg 3 mL column AFFINILUTE MIP PAH 25 mg 3 mL column ISOLUTE SAX PSA 1 9 6 mL column ISOLUTE ENV 200 mg 3 mL column AFFINILUTE MIP Triazine 25 mg 10 mL column 904 0030 B 904 0100 C 915 0100 C MO2 0002 B MO2 0002 G M10 0002 B M10 0002 G MO1 0002 G M69 0002 B 611 0005 B 611 0010 B M34 0005 B M73 0002 B 924 0100 C 91
89. analytes functionality etc e Nature of the sample matrix e Degree of purity required e Nature of major contaminants in the sample e Analytical procedure The first factor affecting the choice of a sorbent type or retention mechanism is based on the properties of the analyte i e functional groups of the analyte which can be used for retention on the sorbent There may be a number of different possibilities depending on the analyte characteristics see Table 1 QuickStart Guide to SPE Table 1 Retention mechanism options for various analyte functional groups Analyte functional group Example 8 Retention mechanism option DU ZW NV Hydrophobic non polar alkyl aromatic AGGA Non polar OH SH Polar hydroxyls amines dipoles Basic groups cations Cation exchange Acidic groups anions Anion exchange The choice of sorbent is also influenced by the nature of the sample since certain sample matrices promote analyte retention better by one retention mechanism than another see Table 2 Table 2 Retention mechanism options for various sample types Sample matrix type Example 08 Retention mechanism option Aqueous low ionic strength River water diluted biological fluids Non polar ion exchange Aqueous high ionic strength Sea water biological fluid For extraction of basic or acidic compounds from complex biological fluid samples such as plasma or urine mixed mode SPE columns can provide ad
90. art number Description Quantity 530 0025 P01 ISOLUTE 96 SCX 25 mg plate 1 530 0050 P01 ISOLUTE 96 SCX 50 mg plate 1 530 0100 P01 ISOLUTE 96 SCX 100 mg plate 1 ISOLUTE Array SCX Loose Wells Part number Description Quantity 530 0050 R ISOLUTE Array SCX 50 mg 1 mL wells 100 530 0100 T ISOLUTE Array SCX 100 mg 2 mL wells 100 Pre assembled ISOLUTE Array plates are available To order add the suffix P to the equivalent loose well part number Support Documents TN106 Method Development in Solid Phase Extraction using ISOLUTE SCX and SCX 3 SPE Columns for the Extraction of Aqueous Samples Applications Matrix 0 Analytes Retention Mechanisms Primary Strong cation exchange Primary Strong cation exchange Chemical structure of propylsulfonic acid functional group covalently bonded to the surface of a silica particle Ordering Information ISOLUTE SCX 2 SPE Columns Part number Description Quantity 532 0002 A ISOLUTE SCX 2 25 mg 1 mL 100 532 0005 A ISOLUTE SCX 2 50 mg 1 mL 100 532 0010 A ISOLUTE SCX 2 100 mg 1 mL 100 532 0010 B ISOLUTE SCX 2 100 mg 3 mL 50 532 0020 B ISOLUTE SCX 2 200 mg 3 mL 50 532 0050 B ISOLUTE SCX 2 500 mg 3 mL 50 532 0050 C ISOLUTE SCX 2 500 mg 6 mL 30 532 0050 H ISOLUTE SCX 2 500 mg 10 mL 50 532 0100 B ISOLUTE SCX 2 1 g 3 mL 50 532 0100 C ISOLUTE SCX 2 1 g 6 mL 30 532 0200 D ISOLUTE SCX 2 2 g 15 mL 20 ISOLUTE 96 SCX 2 Fixed Well Plates Part number Description Quantity 532 0025 P01 ISOLUTE 96 SCX 2
91. ate fixed well 1 120 1022 P01 2 mL 20 um un j ISOLUTE Accessories for 96 well Plates Accessories are available for both ISOLUTE 96 and the modular Array format 96 well plates All ISOLUTE 96 well plate products are compatible with the VacMaster 96 Sample Processing Manifold See pages 99 100 for more information ISOLUTE 96 Fixed Well Plates The ISOLUTE fixed well format is an industry standard one piece plate for routine high throughput applications ISOLUTE 96 Fixed Well Plate Sealing Accessories Seal both the inlet and outlet of fixed well plates for storage prior to elution The polypropylene Luer Cap Mat seals the outlet drain holes of the fixed well SPE plate The polypropylene Pierceable Sealing Cap seals the top of the wells of ISOLUTE SPE and collection plates Ordering Information Description Quantity Part number Piercable Sealing Cap 50 121 5204 Luer Cap Mat 25 121 5205 Accessories For SPE and Filtration ISOLUTE Array 96 well plates ISOLUTE Array is a modular 96 well format with individual removal wells for an economical approach to SPE or filtration of variable or low sample numbers Array Accessories Construct individual ISOLUTE Array wells into a microplate format with the Array base plate Seal any unused positions with base plate plugs Align wells or de populate the plate after use with a Well Removing Tool Use Luer Adapters to allow individual wells to be processed on a vacuum proces
92. ators and can be used outside a fume cupboard like the TurboVap LV by using the integral vent tube For solvent extracts in hexane dichloromethane acetonitrile and methanol we recommend a temperature of 40 C as this minimizes thermal degradation and loss of most volatiles by allowing the cool gas to condense components while offering a rapid removal of solvent Systems are available for 40 mL Dionex tubes with stem endpoint detection Upgrade kits to adapt your current TurboVap II are also available Key Applications The main application areas for the TurboVap II are food water soil and environmental solvent extraction TurboVap 500 Volume Range up to 500 mL The TurboVap 500 has two 500 mL end point vessels and can evaporate solvent to a 1 mL or 0 5 mL end point A water bath is used to maintain a stable temperature whilst variable speed fan motors provide for vortex evaporation enabling the unique ability of the cooled glassware to condense and collect the solvent This allows for optimal evaporation conditions to prevent losses as the condensed solvent can be analyzed for carry over Key Applications The ability to take a large solvent sample makes the determination of residue on evaporation possible It can be used in laboratories where access to gas supplies is difficult which makes it ideal for portable installations on marine science boats and use in remote locations If a laboratory has limited fume hood extraction then this i
93. bent Strong cation Extraction of basic analytes from aqueous or partially aqueous exchanger sample matrix Supplied in the protonated form Sorbent of choice if strong cation exchange is primary retention mechanism or if aqueous conditions required for analyte elution For meth ods listing PRS as the SPE sorbent SCX 2 is a direct replacement Strong cation Extraction of basic analytes from aqueous or partially aqueous sam TN106 59 exchanger ple matrix Supplied in the protonated form Sorbent of choice where significant non polar secondary interactions are required For more information on ion exchange SPE procedures see the QuickStart Guide page 174 184 For in depth technical information on the use of ISOLUTE ion exchange sorbents refer to the Chemistry Data Sheets listed in the table above 167 168 Polar Sorbents Isolate analytes or remove interferences from non polar sample matrices using polar retention mechanisms The technical information for Biotage s range of polar sorbents for SPE and sample clean up is summarized on these pages ISOLUTE Silica Based Polar Sorbents ISOLUTE polar silica based sorbent used to isolate analytes from non polar samples using a variety of polar interactions Silica is the most retentive sorbent exhibiting hydrogen bonding interactions vvith the analyte The moisture content of ISOLUTE SI is controlled to 7 1 to aid method robustness ISOLUTE DIOL NH2 and P
94. biotage com SLE 200 HL SLE 400 uL SLE 1 mL SLE 2 mL SLE 5 mL SLE 10 mL Dispense Dispense Dispense Dispense Dispense Dispense pre buffered pre buffered pre buffered pre buffered pre buffered pre buffered sample 200 uL sample 400 uL sample 1 mL sample 2 mL sample 5 mL sample 10 mL Apply vacuum Apply vacuum Apply vacuum Apply vacuum Apply vacuum Apply vacuum 15 Hg 0 5 bar 15 Hg 0 5 bar 15 Hg 0 5 bar for 15 Hg 0 5 bar 15 Hg 0 5 bar 15 Hg 0 5 bar for for 2 10 seconds to 2 10 seconds 2 10 seconds to for 2 10 seconds to for 2 10 seconds to 2 10 seconds to initiate loading to initiate loading jnitiate loading initiate loading initiate loading initiate loading Wait 5 minutes for Wait 5 minutes for Wait 5 minutes for Wait 5 minutes for Wait 5 minutes for Wait 5 minutes for sample to absorb sample to absorb sample to absorb sample to absorb sample to absorb sample to absorb Apply extraction Apply extraction Apply extraction Apply extraction Apply extraction Apply extraction solvent 2 x 500 uL solvent2x1mL solvent 2x 2 5 mL solvent 2 x 5 mL solvent 2 x 10 mL solvent 2 x 1 mL Apply vacuum Apply vacuum Apply vacuum Apply vacuum Apply vacuum Apply vacuum 15 Hg 0 5 bar 15 Hg 0 5 bar 15 Hg 0 5 bar for 15 Hg 0 5 bar 15 Hg 0 5 bar 15 Hg 0 5 bar for for 2 mins to for 2 mins to 2 mins to complete for 2 mins to for 2 mins to 2 mins to complete complete elution co
95. chanisms The technical information for Biotage s range of ISOLUTE ion exchange sorbents is summarized on these pages ISOLUTE Anion Exchange Sorbents ISOLUTE anion exchange sorbents are used for the extraction of acidic compounds VVeak anion exchange sorbents ISOLUTE NH2 and PSA are suitable for the isolation of strongly acidic analytes e g those containing sulfonic acid groups or analytes that are unstable under acidic conditions The sorbents positive charge can be eliminated by pH control for analyte elution ISOLUTE Anion Exchange Sorbents Description Average Particle Pore Size Hm Diameter A Aminopropyl Si CH 3NH 60 PSA Ethylenediamine N propyl Si CH gt 3NH CH gt 2NH gt non endcapped SAX Quaternary amine chloride Si CH5 3N CH cr counter ion PE AX Quaternary amine acetate Si CH 3N CH3 3 OAc counter ion lon Exchange Sorbents Strong anion exchange sorbents ISOLUTE SAX and PE AX are more suited to the extraction of weakly acidic compounds as they retain a permanent positive charge across the pH range Analytes are eluted from strong anion exchange sorbents by eliminating the charge on the analyte ISOLUTE PE AX is supplied with an acetate counter ion This has a weaker affinity with the sorbent s quaternary amine functional group compared to the chloride counter ion used in ISOLUTE SAX ISOLUTE PE AX may therefore provide more efficient extracti
96. contaminate samples For ordering information see page 102 Environmental Applications and Products for Environmental Analysis PTFE Extraction Caps Directly connect screw top sample bottles to 6 or 70 mL ISOLUTE SPE columns with PTFE caps Available in two sizes to fit standard European 15045 or US 33 400 style threads and ideal for use in Oil amp Grease determination where minimal sample transfer is beneficial Ordering Information Description Quantity Part number PTFE Extraction Cap 1SO45 thread 5 121 2085 PTFE Extraction Cap 33 400 thread 5 121 2086 VacMaster Drying Adapter During environmental sample preparation applications it is often necessary to elute analytes vvith a vvater immiscible solvent It is important to thoroughly dry the sorbent bed before elution to obtain high reproducible analyte recoveries The VacMaster Drying Adapter significantly reduces drying times compared vvith vacuum aspirated air flovv Use vvith any suitable gas e g nitrogen air for fast efficient SPE column drying For ordering information see page 101 Rapid Tracee SPE Workstation The RapidTrace SPE Workstation is a modular highly scalable automated sample preparation platform designed for high throughput Units are available to accommodate 1 mL and 3 mL or 6 mL industry standard SPE cartridges and can process up to 100 samples in less than two hours unattended Each unit can support 1 mL and 3 mL with 1
97. d development time and enhancing productivity With the increasing use of automation in sample preparation another focus is the development of reliable easily automated techniques and procedures which can maximize the use of liquid handling systems Biotage supplies a range of 96 well and conventional column products compatible with all commonly used automated systems Products for Bioanalytical Sample Preparation Technique Products Analyte functionality Extract cleanliness Features advantages Protein Precipitation PPT Supported Liquid Extraction SLE Solid Phase Extraction SPE Molecularly Imprinted Polymers SPE Analytes must be soluble in water immiscible solvents ISOLUTE PPT Protein Precipitation Plates ISOLUTE SLE Supported Liquid Extraction Plates ISOLUTE and EVOLUTE SPE Products AFFINILUTE MIPs Simultaneous extraction of acidic basic or neutral compounds Acidic basic and neutral e Acidic and neutral e Basic and neutral e Simultaneous extraction of acidic basic or neutral compounds e Acidic only e Basic only e Class fractionation High selectivity based on the structure of the analyte of interest Protein removal only Dependent on choice of extraction solvent Dependant on sorbent choice Improved clean up compared with protein precipitation Lower detection limits due to cleaner extracts A fast and simple technique for removal of
98. dic basic and or neutral compounds from biological fluid samples The hydrophobic character of the non polar sorbent decreases with chain length C18 and C8 are commonly used non polar sorbents capable of extracting compounds of wide ranging polarity Extract cleanliness can be enhanced for compounds with significant non polar character by using a shorter chain length non polar sorbent e g ISOLUTE C2 This will allow more polar interferences to be removed from the column prior to analyte elution Request Chemistry Data Sheet TN126 for more details The secondary interactions provided by non endcapped non polar sorbents can enhance the extraction of basic drugs from biological fluids giving higher recoveries and cleaner extracts than endcapped non polar sorbents Request Chemistry Data Sheet TN112 for more details See page 30 37 156 159 for more details and ordering information ISOLUTE Mixed mode SPE Products A range of ISOLUTE mixed mode SPE sorbents are available providing mixed mode retention mechanisms suitable for extraction of most drug s and metabolites Selection of mixed mode generic SPE sorbent by analyte class Class HAX HCX HCX 3 HCX 5 HCX Q Acidic Basic Basic lipophilic Basic pK gt 11 Polybasic aOptimum sorbent for basic drugs where it is difficult or impossible to eliminate positive charge s using pH See pages 46 51 160 163 for more details and ordering information 12 N
99. ding integral depth filters are available fitted into selected ISOLUTE SPE columns The depth filter can be left in place during analyte elution enabling recovery of particulate bound analytes The depth filter material is highly solvent resistant and will not contaminate the final extract Ordering Information Description Quantity Part number ISOLUTE C18 500 mg 6 mL with integral depth filter 30 220 0050 CD ISOLUTE C18 1 g 6 mL with integral depth filter 30 220 0100 CD ISOLUTE C18 EC 1 g 6 mL with integral depth filter 30 221 0100 CD ISOLUTE ENV 200 mg 6 mL with integral depth filter 30 915 0020 CD ISOLUTE Depth Filter Reservoirs for connecting to a standard ISOLUTE SPE column are also available see page 86 for ordering information Custom Manufacturing Options Your application may require alternative product configurations to those listed in this section Through our Custom Manufacturing Service we can supply alternative configurations including e SPE columns fitted with stainless steel frits for additional extract purity e Glass SPE columns with PTFE frits e SPE columns fitted with integral depth filters Please see pages 187 189 for details of our Custom Manufacturing Service VacMaster LVE Kit Designed for use with VacMaster Sample Processing Manifolds the Large Volume Extraction LVE kit enables up to ten large volume samples to be loaded simultaneously Constructed from PTFE components the VacMaster Kit will not
100. ditional clean up Practical Aspects of SPE Method Development Once an appropriate sorbent type retention mechanism has been selected this guide provides a summary of the more important points to consider during method development The table on page 180 182 summarizes this information for non polar polar ion exchange and mixed mode retention mechanisms An SPE procedure typically consists of 6 steps see Figure 3 Sample pre treatment SPE column solvation SPE column equilibration Sample application SPE column washing interference elution step O U A U N m Analyte elution from the SPE column 107 QuickStart Guide to SPE ANALYTE INTERFERENCES e Figure 3 Typical SPE procedure If using the SPE column to retain interferences while allowing analytes to pass straight through follow the same guidelines but use conditions to promote retention of interferences rather than analytes during steps 1 4 Collect the sample once it has passed through the SPE column as this will contain the analytes Flow rate control Flow rate control during sample loading interference and analyte elution steps is important Too fast a flow rate can result in low recoveries due to breakthrough during the analyte retention sample loading step or inade quate elution during the elution step Too high a flow rate during the interference elution
101. e Ordering Information ISOLUTE C8 EC SPE Columns Part number Description Quantity 291 0010 A ISOLUTE C8 EC 100 mg 1 mL 100 291 0010 B ISOLUTE C8 EC 100 mg 3 mL 50 291 0010 G ISOLUTE C8 EC 100 mg 10 mL 50 291 0020 B ISOLUTE C8 EC 200 mg 3 mL 50 291 0020 H ISOLUTE C8 EC 200 mg 10 mL 50 291 0050 B ISOLUTE C8 EC 500 mg 3 mL 50 291 0050 C ISOLUTE C8 EC 500 mg 6 mL 30 291 0100 C ISOLUTE C8 EC 1 g 6 mL 30 291 0200 D ISOLUTE C8 EC 2 g 15 mL 20 291 0500 E ISOLUTE C8 EC 5 g 25 mL 20 ISOLUTE 96 C8 EC Fixed Well Plates Part number Description Quantity 291 0025 P01 ISOLUTE 96 C8 EC 25 mg plate 1 291 0050 P01 ISOLUTE 96 C8 EC 50 mg plate 1 291 0100 P01 ISOLUTE 96 C8 EC 100 mg plate 1 ISOLUTE Array C8 EC Loose Wells Part number Description Quantity 291 0025 R ISOLUTE Array C8 EC 25 mg 1 mL wells 100 Support Documents TN101 Method Development in Solid Phase Extraction using Non polar ISOLUTE SPE Columns for the Extraction of Aqueous Samples See pages 156 and 157 for additional technical information about ISOLUTE C8 EC Applications Matrix Analytes Retention Mechanisms Primary Non polar Aqueous Wide polarity range Secondary Polar and weak cation exchange Ordering Information ISOLUTE C6 SPE Columns Part number Description 380 0010 A ISOLUTE C6 100 mg 1 mL 380 0010 B ISOLUTE C6 100 mg 3 mL 380 0020 B ISOLUTE C6 200 mg 3 mL 380 0050 B ISOLUTE C6 500 mg 3 mL 380 0050 C ISOLUTE C6 500 mg 6 mL 380 0
102. e 28 4 kor tei Aha d PASE Hataetidr Arche ii O 1 Erd Tapper Trl Para Put je Ley Farticin Sine Analysis Sori Recovery Tes imRE AT Se ria Packed Column and Component Test Fea Beale Pass Cu WAP m Fer a FASE Prot Puri Teri ci Path klalerui Weigh De FALSE A je Parc Tara Li sa MIA hye ende TEA el ua 0 04 Bee of Parties 3 773 F R r b s m yib Perera l Purtriru EFH 053 40 Het RES l 5 j i p Par qa dl r 2 ei ve apa Bath deere lara Ea m Bu ii L F mn dos eli Era 1 m urn oem pie CRE he n ml m n Emi pe pe Fe q er m ma l bil Gee Ai rt rar H in VE in pusquya ra mi ag de Reproducible optimized chemistry for ISOLUTE and EVOLUTE sorbents e Minimize method development time Eliminate need for time consuming method changes vvhen different batches of sorbent are used High and reproducible capacities of ISOLUTE ion exchange sorbents Reproducible high recoveries in ion exchange SPE vvithout using columns with excessively large sorbent mass Saves time money and increases analyte concentration in final extract High purity sorbents frits columns and 96 vvell SPE plates e The purity of all the components used to manufacture ISOLUTE and EVOLUTE SPE products are monitored to ensure compatibility with the most demanding applications Samp
103. e retention or e Poor analyte elution To identify inadequate retention use one of the following methods a Stack two pre conditioned and equilibrated columns together using an adaptor see page 86 and load the sample Separate the columns and elute analyte from the lower column b Alternatively collect the eluent during sample load from a single SPE column and re extract using a known method e g liquid liquid extraction If analyte is present on the second SPE column a or in the liquid liquid extraction solvent b the analyte is not sufficiently retained on the SPE column To identify inadequate elution a Elute analyte with up to 10 bed volumes of chosen elution solvent Repeat If a significant amount of analyte is present in the second elution the analyte is not being eluted efficiently from the column Please don t hesitate to contact 1 Point Support for further assistance in SPE method development or troubleshooting Detailed Chemistry Data Sheets for all the sorbents mentioned in this QuickStart Guide are available see sor bent ordering pages for details QuickStart Guide to SLE ISOLUTE SLE Method Development Guide Biotage has examined a variety of acidic basic and neutral compounds to determine recovery levels utilizing ISOLUTE SLE By screening four different loading pH s and four different extraction solvents we were able to determine that recoveries were optimum when the pH was near the pKa of the ana
104. echanisms allow the use of rigorous interference elution regimes providing extremely clean extracts and reducing matrix effects in LC MS analysis Technical information for EVOLUTE and ISOLUTE mixed mode sorbents are summarized on these pages Mixed mode SPE Analytes are initially retained by a non polar retention mechanism with inorganic salts polar interferences and high molecular weight peptides and proteins passing directly through the sorbent bed The pH is then adjusted to allow an ion exchange retention mechanism to retain the analyte Non polar interferences are removed using an organic solvent or organic solvent water wash as shown in Figure 1 Salts phospholipids and Proteins etc excluded from small non ionizable Drugs are eluted _ Clean extract the pores and pass to waste interferences are removed E is obtained Interference Elution 1 Interference Elution 2 Analyte Elution Analysis Figure 1 EVOLUTE Mixed mode polymer based SPE products For a full revievv of mixed mode SPE using the EVOLUTE family of polymer based SPE products please request a copy of the EVOLUTE User s guide or download from www biotage com ISOLUTE Mixed mode silica based SPE products ISOLUTE HCX HCX 3 8 HCX 5 Silica based mixed mode SPE for Basic Drugs Biotage offers three silica based mixed mode sorbents for extraction of basic drugs Compared to resin based mixed mode sorbents these have reduced non polar charac
105. ed water wettable polymer based sorbent that extracts acidic basic and neutral analytes from biological fluids and other aqueous matrices A generic approach can be adopted to extract a wide range of compounds from a range of matrices thus reducing method development time while producing super clean extracts EVOLUTE CX is a mixed mode polymer based SPE sorbent for the isolation of basic drugs from biological fluids ideal for the extraction of forensically significant drugs of abuse from urine saliva or blood Optimized sorbents provide clean extracts with minimal problematic LC MS MS matrix effects due to proteins salts and phospholipids See www biotage com applications for application note AN702 Extraction of Basic Drugs from Plasma using EVOLUTE CX EVOLUTE VVCX is a vveak cation exchange mixed mode polymer based SPE sorbent for extraction of basic drugs including quaternary amines using acidic elution conditions EVOLUTE AX a mixed mode strong anion exchange sorbent for selective extraction of acidic analytes EVOLUTE VVAX a mixed mode vveak anion exchange sorbent for selective extraction of acidic analytes including phosphates and sulphonic acids See page 21 26 for ordering information ISOLUTE SLE Supported Liquid Extraction ISOLUTE SLE Supported Liquid Extraction plates and columns offer an efficient alternative to traditional liquid liquid extraction LLE and can cut sample preparation time in half Utilizing a gener
106. ent the column should first be thoroughly dried using centrifugation or aspirating air or CO for at least 15 20 mins using vacuum or positive pressure 180 QuickStart Guide to SPE Mixed mode polymer based sorbents see page 22 27 160 163 EVOLUTE CX Methanol Hu il Conditioning Equilibration Sample pH Analyte Elution Elution EVOLUTE VVCX A Quats and strong bases only EVOLUTE VVCX B combined vveak and strong bases EVOLUTE AX A plasma samples EVOLUTE AX B urine or other high ionic strength matrix Methanol Methanol Methanol Methanol EVOLUTE VVAX Methanol Mixed mode silica based sorbents see page 46 50 160 163 ISOLUTE HAX Methanol ISOLUTE HCX HCX 3 HCX 5 Methanol ISOLUTE HCX Q Methanol Non polar silica based sorbents see page 32 45 156 159 ISOLUTE C18 C18 EC MFC18 C8 C8 EC C6 C4 C2 C2 EC PH PH EC CH EC CN CN EC Methanol or Acetonitrile Ammonium acetate 0 05M pH 6 Ammonium hydroxide 5 v v Ammonium acetate 0 05M pH 7 Ammonium hydroxide 2 v v Formic acid 2 v v Formic acid pH 2 2 V V Ammonium acetate 0 05M pH 6 Ammonium acetate 0 05M pH 7 VVater and or 20 50 mM buffer the same pH as the sample Dilute with ammonium acetate 0 05M pH 6 1 3 v v Dilute with ammonium hydroxide 5 1 3 v v Dilute with ammonium acetate 0 05M pH 7 1 3
107. ents 2012 The following trademarks are owned by Biotage AB Biotage Advancer AFFINILUTE Endeavor EVOLUTE ExploraSep m Firefly design FLASH FlashMaster FlashVac HPFC HP SIL Initiator Isolera ISOLUTE IST UY IST design Kiloprep KP C18 HS KP C18 VVP KP C4 VVP KP NH KP Sil MIP4SPE MIP Rule of 6 MIP 4 Process MIP 4 Proteins PathFinder RapidTrace Resolux Samplet SIM SNAP SP1 SP4 Syro Wave TurboVap VacMaster V 10 ZIF ZIP SIM and 1 Point Support Notes Notes Notes NORTH AMERICA 1 800 446 4752 1 704 654 4900 tel 1 434 296 8217 fax ordermailbox biotage com EUROPE 800 18 56 57 10 46 18 56 57 10 tel 46 18 56 57 05 fax order biotage com JAPAN 81 3 5627 3123 tel 81 3 5627 3121 fax jp_order biotage com CHINA 86 21 2898 6655 tel 86 21 2898 6153 fax cn_order biotage com To locate a distributor please visit our web site at www biotage com CAT1006 0112 NP 2012 Biotage All rights reserved All brand and product names are trademarks or registered trademarks of their respective companies The information contained in this document is subject to change without notice M Biotage CD www biotage com sampleprep
108. eries eliminates emulsion formation and reduces sample preparation time by half Each ISOLUTE SLE well and column contains a highly modified form of diatomaceous earth When the aqueous biological fluid sample is applied it spreads over the surface of the support and is absorbed The analytes remain on the surface of the support forming the interface for the extraction equivalent to the phase interface in LLE When the water immiscible extraction solvent is applied analytes are efficiently desorbed and the solvent is collected This process is shown schematically in Figure 1 ISOLUTE SLE 96 well plates and columns are optimized for the simultaneous extraction of aqueous samples using a generic methodology for neutral acidic or basic compounds Plates and columns can be processed manually using positive pressure or vacuum manifolds see pages 94 100 for details or a variety of automated liquid handling systems Sample is added to the plate or column and vacuum or pressure applied to load the sample The aqueous portion is absorbed onto the sorbent for 5 minutes but unlike SPE sample does NOT flow through the plate A water immiscible solvent is then applied to the plate desorbing the analytes of interest which are collected in a collection plate tube Extracts can then be evaporated and reconstituted in a solvent suitable for analysis generally by LC MS MS ISOLUTE SLE is available in formats suitable for processing from 100 u
109. es EVOLUTE WCX a mixed mode weak cation exchange sorbent for selective extraction of basic analytes including quaternary amines using acidic elution conditions EVOLUTE AX a mixed mode strong anion exchange sorbent for selective extraction of acidic analytes EVOLUTE WAX a mixed mode weak anion exchange sorbent for selective extraction of acidic analytes including phosphates and sulphonic acids Ordering information for EVOLUTE products can be found on pages 21 27 Environmental Applications and Products for Environmental Analysis AFFINILUTE MIP Due to the complex nature of environmental samples like vvaste vvater clean up may require a higher level of selectivity to allovv for the trace levels of detection required AFFINILUTE MTPs offer this selectivity for a num ber of environmentally significant compounds through their unique molecularly imprinted technology Description Quantity Part number AFFINILUTE MIP f blockers 25 mg 10 mL 50 M18 0002 G AFFINILUTE MIP B blockers 25 mg 3 mL 50 M18 0002 B AFFINILUTE MIP NSAIDs 25 mg 3 mL 50 M72 0002 B AFFINILUTE MIP NSAIDs 25 mg 10 mL 50 M72 0002 G AFFINILUTE MTP Triazines 25 mg 10 mL 50 08 0002 For specific methods please see vvvvvv biotage com ISOLUTE Layered SPE Columns for Extracting Multiple Analyte Suites Ideal for applications vvhere analytes vvith a broad range of polarity are to be extracted from a single sample ISOLUTE layered SPE columns extend the range of analyt
110. es extracted using a single column Extract a range of endocrine disrupters or combined suite of organochlorine triazine and organophosphorus pesticides simultaneously with layered columns Costs can be reduced and productivity improved as fevver samples need to be prepared for each assay Ordering Information Description Quantity Part number ISOLUTE 2 18 500 mg 3 mL 50 933 0050 B ISOLUTE C2 C18 EC 1 g 6 mL 30 933 0100 C ISOLUTE C8 ENV 400 mg 6 mL 30 934 0040 C ISOLUTE C18 ENV 400 mg 6 mL 30 935 0040 C 137 138 Environmental Applications and Products for Environmental Analysis ISOLUTE EPH SPE Columns ISOLUTE EPH SPE columns have been optimized for the fractionation of extractable petroleum hydrocarbons in soil extracts prior to analysis by GC The columns and methodologies have been optimized to provide a clean fractionation of the aliphatic and aromatic fractions in soil extracts vvith no breakthrough of the lower MW polycyclic aromatic hydrocarbons into the aliphatic fraction The columns are quality controlled to ensure consistent fractionation from batch to batch Application Note AN704 RapidTrace Procedure for the Automated Fractionation of Extractable Petroleum Hydrocarbons EPH in Soil using ISOLUTE EPH SPE Columns Application Note AN703 ASPEC Procedure for the Automated Fractionation of Extractable Petroleum Hydrocarbons EPH in Soil using ISOLUTE EPH SPE Columns Ordering Information
111. esults in a surface vvith decreased capacity for basic compounds Compounds are retained by ion exchange with the positively charged surface or by specific interactions with the metal center ISOLUTE AL N Neutral surface allows interactions of the aluminium metal center with compounds whose heteroatoms are electronegative e g N O P 8 S or whose highly aromatic structure make them electron rich The adsorbent can be useful for retaining amines and aromatic compounds from either aqueous or non aqueous solvents ISOLUTE AL B Washing this material with a basic solution results in a net negative charge Cationic compounds are retained on the negatively charged surface or by specific interactions with the metal center Ordering Information Column Part numbers configuration Quanity AL A AL N AL B 100 mg 1 mL 100 713 0010 A 714 0010 A 715 0010 A 200 mg 1 mL 100 713 0020 A 714 0020 A 715 0020 A 200 mg 3 mL 50 713 0020 B 714 0020 B 715 0020 B 500 mg 3 mL 50 713 0050 B 714 0050 B 715 0050 B 500 mg 6 mL 30 713 0050 C 714 0050 C 715 0050 C 1 g 3 mL 50 713 0100 B 714 0100 B 715 0100 B 1 g 6 mL 30 713 0100 C 714 0100 C 715 0100 C 2 g 6 mL 30 714 0200 C 2 g 15 mL 20 713 0200 D 714 0200 D 715 0200 D 5 g 25 mL 20 713 0500 E 714 0500 E 715 0500 E 10 g 70 mL 16 714 1000 F 715 1000 F See pages 170 and 171 for additional technical information about ISOLUTE AL A AL N and AL B ISOLUTE Bulk SPE Sorbents ISOLUT
112. etabolites fluids 25 mg fixed well plate AN700 Diuretics Urine EVOLUTE ABN 610 0010 H Clinical 26 50 um 100 mg 10 mL column AN718 Ethyl Glucuronide Urine EVOLUTE AX 603 0025 PO1 Forensic 25 EtG 25 mg fixed vvell plate and other formats AN733 Melamine Biological EVOLUTE CX 611 0005 B Clinical 26 fluids 50 mg 3 mL column AN732 Melamine Milk EVOLUTE CX 611 0005 B Food 26 50 mg 3 mL column AN743 Mephedrone Biological EVOLUTE CX 601 0025 P01 Forensic 25 fluids 25 mg fixed well plate AN737 Organophosphate Urine EVOLUTE VVAX 604 0025 P01 Clinical 25 Pesticide 25 mg fixed Metabolites well plate AN701 Pharmaceuticals VVater EVOLUTE ABN 610 0020 C Environmental 25 50 um 200 mg 6 mL column Visit the Sample Preparation Application Database at www biotage com applications A comprehensive user guide is available detailing EVOLUTE EVOLUTE User s Guide generic methods along with practical advice on method optimization and robust application development To request a copy visit www biotage com 27 do NER Tas J pe ISOLUTE SPE Columns and g6 vvell Plates Applications Matrix Analytes Retention Mechanism CH CH ane H o cy CH O r OG q A Aqueous Wide polarity range Primary Non polar Le CH qu NT Ho y Chemical structure of ISOLUTE ENV a hydroxylated polystyrene divinylbenzene co polymer Ordering Information ISOLUTE ENV SPE
113. f non polar and ion exchange interactions SPE sorbents in this catalog are classified by retention mechanism Each sorbent offers a unique combination of these properties which can be applied to a wide variety of specific extraction problems 175 QuickStart Guide to SPE Selectivity This extensive range of sorbent chemistries provides one of the most powerful aspects of SPE high selectivity Selectivity is the degree to which an extraction technique can separate the analyte of interest from the interfer ences in the original sample The highly selective nature of SPE is due to two factors First each extraction sorbent chemistry offers unique and distinctive retention properties to address a wide range of analyte charac teristics The second factor is best understood by comparison with liquid liquid extraction In liquid liquid extrac tion the two liquids phases must be immiscible with each other Clearly an aqueous sample cannot be extracted with methanol In SPE however one phase is a solid support or sorbent and is therefore by defini tion immiscible with any extraction solvent used This results in a huge variety of possible sorbent solvent combinations with potential to achieve highly selective extractions Capacity The capacity of a the sorbent is defined as the total mass of strongly retained analyte that can be retained by a given sorbent mass under optimum conditions When determining the amount of sorbent requ
114. formate or hydroxide buffers are recom mended 20 50 mM buffer the same pH as the sample 20 50 mM buffer the same pH as the sample At least 2 pH units below the pK of the analyte At least 2 pH units below the pK of the analyte pH gt 6 8 and 2 pH units below the analyte pK At least 2 pH units above the pK of the analyte At least 2 pH units above the pK of the analyte lt 7 8 and 2 pH units above the analyte pK QuickStart Guide to SPE Interference Elution Non polar solvent which may contain a low concentra tion of a polar modifier that will not elute the analyte e g hexane and ethyl acetate or iso propanol Maintain the same pH as the sample and evaluate the addition of 20 40 methanol to elute non polar interferences methanol can be increased for additional extract cleanliness Check for analyte break through Maintain the same pH as the sample and evaluate the addition of 20 70 methanol to elute non polar interferences methanol can be increased for additional extract cleanliness Check for analyte breakthough Maintain the same pH as the sample and evaluate the addition of 20 50 methanol to elute non polar interferences Maintain the same pH as the sample and evaluate the addition of 20 50 methanol to elute non polar interferences Maintain the same pH as the sample and evaluate the addition of 20 50 methanol to elute no
115. from a non polar sample matrix using hydrogen bonding retention mechanism Less retentive sorbent than SI Can also be used as a weak anion exchange sorbent in aqueous and non polar sample matrices For more information on polar SPE procedures see the QuickStart Guide page 174 184 For in depth technical information on the use of ISOLUTE polar sorbents refer to the Chemistry Data Sheets listed in the table above 169 170 Polar Sorbents ISOLUTE FL ISOLUTE FL is generally used to clean up non polar solvent extracts containing non polar analytes e g chlorinated pesticides Polar interferences are retained on the column vvhile analytes are eluted ISOLUTE AL A AL N amp AL B A high activity 50 200 um particle size range of aluminas available in acidic neutral and basic surface pH options ISOLUTE Polar Sorbents Description Average Pore Particle Size Diameter Hm A Florisil PR MgO 3 6 SiO 0 1 OH 150 250 60 AL A Alumina acidic 50 200 120 AL N Alumina neutral 7 50 200 120 AL B Alumina basic 50 200 120 Polar Sorbents The surface of the alumina can absorb molecules by interaction with the aluminium metal center hydrogen bonding vvith surface hydroxyl groups or by ion exchange if the surface carries a charge The extent of these different interactions can be enhanced by control of the surface pH by treatment with acidic basic or neutral solutions
116. g plate 1 ISOLUTE Array PE AX Loose Wells Part number Description Quantity 503 0050 R ISOLUTE Array PE AX 50 mg 1 mL wells 100 503 0100 T ISOLUTE Array PE AX 100 mg 2 mL wells 100 Si O 5i CH N CH5 O V CHSCOO OH Chemical structure of PE AX silane covalently bonded to the surface of a silica particle Pre assembled ISOLUTE Array plates are available To order add the suffix P to the equivalent loose well part number Support Documents TN103 Method Development in Solid Phase Extraction using ISOLUTE PE AX and SAX SPE Columns for the Extraction of Aqueous Samples See pages 164 and 165 for additional technical information about ISOLUTE PE AX m gt mi a b 1 Applications N Matrix 0 Analytes Retention Mechanisms Si O Si CH COOH Aqueous Cationic Primary Weak cation exchange Sj OH Primary Weak cation exchange Chemical structure of CBA silane covalently bonded to the surface of a silica particle Ordering Information ISOLUTE CBA SPE Columns Part number Description Quantity 520 0002 A ISOLUTE CBA 25 mg 1 mL 100 520 0005 A ISOLUTE CBA 50 mg 1 mL 100 520 0010 A ISOLUTE CBA 100 mg 1 mL 100 520 0010 B ISOLUTE CBA 100 mg 3 mL 50 520 0020 B ISOLUTE CBA 200 mg 3 mL 50 520 0050 B ISOLUTE CBA 500 mg 3 mL 50 520 0050 C ISOLUTE CBA 500 mg 6 mL 30 520 0100 C ISOLUTE CBA 1 g 6 mL 30 520 0200 D ISOLUTE CBA 2 g 15 mL 20 ISOLUTE 96 CBA Fixed Well Plates Part number
117. h standard solid phase extraction techniques The molecularly imprinted technology allows specific analytes to be extracted at much lower detection limits than previously possible and has been developed to target analytes that have traditionally been difficult to clean up at low levels See page 15 19 for more details Solid Phase Extraction SPE Products Biotage manufactures a comprehensive range of solid phase extraction products These include both EVOLUTE and ISOLUTE polymer based and silica based sorbents which have different but complementary extraction characteristics The range includes sorbents offering a choice of non polar mixed mode and ion exchange retention mechanisms for extraction of drugs and metabolites from biological fluids Depending on the assay requirements the SPE method can be tailored to deliver high recoveries of a range of acidic basic and neutral drugs using a single generic method or highly selective extraction of a single drug with a very clean extract that exhibits minimal ion suppression EVOLUTE Sample Preparation Products EVOLUTE SPE columns and plates are ideal for extraction of drugs and metabolites from biological fluid samples EVOLUTE sorbents have a uniquely modified surface structure that is water wettable to promote robust reliable sample preparation They also have an optimized pore structure that minimizes the retention of high molecular weight endogenous materials present in complex sample
118. he QuEChERS technique involves two stages i a crude extraction and partitioning step using organic solvent and a salt solution ii followed by further extraction and clean up using dispersive solid phase extraction Using Biotage QuEChERS tubes analysts no longer need to weigh individual components which makes the method quicker and more reliable Biotage QuEChERS products include extraction tubes for the initial extraction and partitioning of analytes from homogenized food samples and clean up tubes for the dispersive solid phase extraction All Biotage QuEChERS products come packed in a rack for ease of storage and use on the bench Additionally solvent resistant paper labels aid hassle free sample identification AOAC 2007 01 and European EN 15662 Methodologies Biotage supplies pre weighed extraction and clean up tubes that conform with AOAC and the EU published methods including those for waxed and highly pigmented fruit and vegetable samples Analyte Recovery Analyte Recovery m AOAC Pigmented Fruit M EN Pigmented Fruit and Veg Broccoli and Veg Broccoli m AOAC General Fruit M EN General Fruit Figure 1 and Veg Apple and Veg Apple Figure 2 Figures 1 and 2 Pesticide suite was extracted using Biotage QuEChERS products and the relevant methodology AOAC or EN for apple fig 1 and broccoli fig 2 In all instances recoveries were above 75 References 1 AOAC Official Method 2007 01 Pesticide res
119. his step 179 QuickStart Guide to SPE Step 6 Analyte Elution A good elution solvent should elute the analyte in as low a volume as possible Choose a solvent or solvent mixture in which the analyte is highly soluble Elution solvents should overcome both primary and any secondary interactions by which the analyte is retained Table 3 provides some useful guidelines on the choice of analyte elution solvents During method development it is important to evaluate the effect of different flow rates on the efficiency of analyte elution and specify the optimum flow rate range in the protocol Lower flow rates are often required for ion exchange sorbents It is sometimes useful to elute the analyte with two aliquots of X 2 mL of solvent rather than a single elution step with X mL solvent Where basic analytes have been extracted using a non polar non endcapped sorbent the elution solvent should be optimized to also overcome secondary interactions between surface silanol groups and the positively charged or protonated analyte This can be achieved by acidifying the elution solvent to suppress silanol ionization or increasing the pH to eliminate the positive charge on the basic analyte Alternatively elute with solvent containing 1 1M ammonium acetate Table 3 Suggestions for the use of EVOLUTE and ISOLUTE Non polar Polar Ion Exchange and Mixed mode Sorbents Interference Elution Analyte Elution Conditioning Equilibration Samp
120. holamines Plasma RapidTrace and EVOLUTE VVCX 612 0005 B 26 50 mg 3 mL column AN745 Catecholamines Plasma EVOLUTE VVCX 602 0025 P01 25 25 mg Array fixed well plate IST1071A Catecholamines Urine ISOLUTE MFC18 100 mg 10 mL column 240 0010 G 34 For automation compatibility ISOLUTE MFC18 100 mg 1 mL column 240 0010 A 34 AN602 Corticosteroids Plasma ISOLUTE SLE 200 820 0200 P01 76 Supported Liquid Extraction plate AN728 Cyclosporine Whole blood RapidTrace and 460 0050 B 60 ISOLUTE SI 500 mg 3 mL column AN700 Diuretics Urine EVOLUTE ABN 50 um 610 0010 H 26 100 mg 10 mL column AN733 Melamine Biological EVOLUTE CX 611 0005 B 26 fluids 50 mg 3 mL column AN713 NNAL Urine AFFINILUTE MIP 25 mg 3 mL or M06 0002 B 19127 25 mg 10 mL column M06 0002 G 19 127 AN603 Non steroidal Anti Plasma ISOLUTE SLE 200 820 0200 PO1 76 inflammatory Drugs Supported Liquid NSATDs Extraction plate IST1008S Pyroxidine Riboflavin Biological ISOLUTE CBA 520 0020 B 56 and Thiamine matrices 200 mg 3 mL column 132 Applications and Products for Forensic and Clinical Analysis Forensic and Clinical Clinical Applications continued Application number AN721 AN722 AN740 AN601 AN726 AN712 AN724 AN735 AN734 Analyte Tamoxifen Tamoxifen Testosterone and other steroid hormones Tricyclic Antidepressants Tricyclic Antidepressants TSNAs Vitamin D Vitamin D and metabolites VVarfarin
121. i CH CH ISOLUTE HCX Q combines C8 Octyl and carboxylic acid functionalities Ordering Information ISOLUTE HCX Q SPE Columns Part number Description Quantity 986 0002 A ISOLUTE HCX Q 25 mg 1 mL 100 986 0005 A ISOLUTE HCX Q 50 mg 1 mL 100 986 0010 A ISOLUTE HCX Q 100 mg 1 mL 100 ISOLUTE 96 HCX Q Fixed Well Plates Part number Description Quantity 986 0025 P01 ISOLUTE 96 HCX Q 25 mg plate 1 986 0100 P01 ISOLUTE 96 HCX Q 100 mg plate 1 ISOLUTE Array HCX Q Loose Wells Part number Description Quantity 986 0025 R ISOLUTE Array HCX Q 25 mg 1 mL wells 100 986 0100 T ISOLUTE Array HCX Q 100 mg 2 mL wells 100 Pre assembled ISOLUTE Array plates are available To order add the suffix P to the equivalent loose well part number Support Documents TN129 Generic Method for the Extraction of Quaternary Amine and Polybasic Drugs from Biological Fluids using ISOLUTE HCX Q SPE Columns and 96 well Plates See pages 162 and 163 for additional technical information about ISOLUTE HCX Q Applications Matrix Analytes Retention Mechanisms Si 0 i CH N CH CI Aqueous Non polar Primary Non polar and and anionic strong anion exchange Si O Si CH CH ISOLUTE HAX combines C8 Octyl and quaternary amine functionalities Ordering Information ISOLUTE HAX SPE Columns Part number Description Quantity 903 0005 A ISOLUTE HAX 50 mg 1 mL 100 903 0010 A ISOLUTE HAX 100 mg 1 mL 100 903 0020 B ISOL
122. ic analytes e g quaternary amines or analytes that are unstable under basic conditions The sorbent s negative charge can be eliminated by pH control for analyte elution Strong cation exchange sorbents ISOLUTE SCX 2 and SCX 3 are used for extraction of vveakly basic compounds as they retain a permanent negative charge across the pH range Analytes are eluted from strong cation exchange sorbents by eliminating the charge on the analyte ISOLUTE Cation Exchange Sorbents Description Average Particle Pore Diameter 2 Size um Propylcarboxylic acid Si CH z COOH Propylsulfonic acid Si CH gt 3503 H Ethylbenzene sulfonic acid Si CH2 2 O 2 SO H lon Exchange Sorbents ISOLUTE SCX 2 contains a propylsulfonic acid group This sorbent exhibits minimal non polar secondary interactions and analytes can often be eluted using purely aqueous conditions ISOLUTE SCX 3 contains an ethylbenzene sulfonic acid group This exhibits significant non polar secondary interactions and may be more suitable for extraction of analytes with low hydrophobic character Elution solvents should contain some organic component along with the appropriate base Sorbent Class Exchange Application Chemistry Ordering Capacity Data Information meq g Sheets Page Weak cation Extraction of strong bases and polybasic compounds from exchanger pK aqueous sample matrix Analyte elution can be performed by 4 8 neutralizing charge on the sor
123. ic and simple methodology it is possible to extract a range of drugs from a variety of biological fluids It is possible to adapt your current liquid liquid methods to SLE improving efficiency and allowing for automation and higher throughput Application note AN723 for THC and metabolites from Plasma using Supported Liquid Extraction SLE prior to LC MS MS Analysis available at www biotage com applications Application note AN720 for the extraction of Vitamin D and Metabolites Evaluation of Supported Liquid Extraction SLE prior to LC MS MS Analysis available at www biotage com applications Application notes AN721 and AN722 for the extraction of Tamoxifen and metabolites from urine and plasma _ respectively are available at www biotage com applications Forensic and Clinical Applications and Products for Forensic and Clinical Analysis AFFINILUTE MIP Biotage AFFINILUTE MTPs are a range of SPE phases based on molecularly imprinted polymers that offer analytical chemists the ultimate in selectivity compared with standard solid phase extraction techniques The selectivity of the molecularly imprinted polymers can be effective in the extraction of drugs that are traditionally difficult to extract due to matrix complexities or analyte structure Description Quantity Part number AFFINILUTE MIP Amphetamines 25 mg 3 mL 50 M28 0002 B AFFINILUTE MIP B blockers 25 mg 10 mL 50 M18 0002 G AFFINILUTE MIP f blockers 25 mg 3 mL 50 M18 0002
124. ica based sorbents it is necessary to add 0 5 5 methanol or iso propanol to the sample in order to maintain solvation of the sorbent bed during sample loading and ensure high analyte recovery Step 2 Column solvation In order for a proper phase interface to exist between the sorbent and the sample most ISOLUTE SPE columns should be solvated prior to sample loading Typical solvents are listed in Table 3 page 180 A typical volume of solvation solvent is 1 mL 100 mg sorbent Step 3 Column equilibration Prior to sample loading the SPE column should be normalized to match the conditions of the pre treated sample This will ensure maximum analyte recovery For example if the sample is pH adjusted to suppress ensure ionization of the analyte equilibrate the column with a buffer of the same pH and ionic strength For weak ion exchange sorbents use buffer pH to also ensure ionization of sorbent for maximum capacity see Table 3 for guidelines for specific sorbents Step 4 Sample loading Extraction efficiency is flow rate dependent so evaluation of the extraction efficiency vs flow rate is a useful part of the method development process See Table 4 page 183 for suggested flow rates Step 5 Interference elution Table 3 provides useful guidelines on the choice of interference elution solvents N B for maximum analyte recovery analyte retention conditions e g pH and ionic strength should be maintained during t
125. idues in foods by acetonitrile extraction and partitioning with magnesium sulfate 2 Anastassiades M Lehotay S J Stajnbaher D and Schenk F J 2003 J AOAC International 86 412 431 3 EN 15662 Foods of plant origin Determination of pesticide residues using GC MS and or LC MS MS following acetonitrile extraction partitioning and clean up by dispersive SPE QUEChERS method QuEChERS Analyte Recovery Analyte Recovery m EN Highly Pigmented Fruit AOAC Waxy Fruit and Veg and Veg Spinach Orange EN Waxy Fruit and Veg Orange Figure 3 Figure 4 Figures 3 and 4 Pesticide suite was extracted using Biotage QUEChERS products and the relevant methodology AOAC or EN for spinach fig 3 and orange fig 4 In all instances recoveries were above 80 Ordering Information Extraction Tubes Ree Na Na Citrate unu Ao Clean up Tubes EE ECC oo o om x om A om Ordering Information Centrifuge Tube di Pack Description Part No Tube Size 50 mL Centrifuge Tube Q0000 50V 50 mL 25 with Rack 71 Supported Liquid Extraction Supported Liquid Extraction ISOLUTE SLE Improve productivity and maximize analyte recovery ISOLUTE SLE Supported Liquid Extraction plates and columns offer an efficient alternative to traditional liquid liquid extraction LLE for bioanalytical sample preparation extracting up to 10 mL of aqueous sample ISOLUTE SLE provides high analyte recov
126. inless steel 188 189 Glass columns and reservoirs 189 Gravity Rack for sample processing under gravity 103 H HAX 96 vvell SPE plates 50 162 163 SPE columns 50 162 163 HCX 96 vvell SPE plates 46 162 163 SPE columns 46 162 163 HCX 3 96 vvell SPE plates 47 162 163 SPE columns 47 162 163 HCX 5 96 vvell SPE plates 48 162 163 SPE columns 48 162 163 HCX Q 96 vvell SPE plates 49 162 163 SPE columns 49 162 163 Hexyl see C6 HM N diatomaceous earth Bulk material 67 Packed columns 79 I Ion exchange sorbents 66 164 167 Ion exchange SPE columns and 96 well plates 52 59 ISOLUTE 96 SPE plates 13 30 32 50 52 59 90 ISOLUTE Array wells and SPE plates 13 30 32 50 52 59 90 91 ISOLUTE Bulk sorbents 66 67 ISOLUTE EPH 138 ISOLUTE HM N diatomaceous earth 67 79 146 ISOLUTE SLE 6 74 78 118 119 126 185 ISOLUTE SPE columns see individual sorbent chemistries For example for ISOLUTE C18 see C18 L Large volume extraction LVE kits 102 140 Layered columns 137 M Matrix solid phase dispersion MSPD 148 Method development assistance 6 MFC18 n Octadecyl monofunctional 96 well SPE plates 34 SPE columns 34 MIP AFFINILUTE MIP 16 19 120 127 137 147 Mixed mode SPE columns 46 50 160 163 Multimode 51 149 Needles PTFE and stainless steel 98 NH2 Aminopropyl 96 well SPE plates 52 Bulk sorbent 66 SPE columns 52 Non polar sorbents 66 156 159 Non polar
127. ion PRESSURE Manifold Service Installation PRESSURE Manifold Platinum Service Contract 1 year PRESSURE Manifold Silver Repair Depot Service Contract 1 year PRESSURE Manifold 9 month extended warranty 1 year total 95 96 PRESSURE 48 and PRESSURE 96 Positive Pressure Manifolds Ordering Information Tabless columns for use with PRESSURE 48 Positive Pressure Manifolds Part number Description Quantity EVOLUTE SPE Columns EVOLUTE ABN 600 0002 AG EVOLUTE ABN 25 mg 1 mL Tabless 100 610 0005 BG EVOLUTE ABN 50 mg 3 mL Tabless 50 EVOLUTE CX 601 0001 AG EVOLUTE CX 10 mg 1 mL Tabless 100 601 0002 AG EVOLUTE CX 25 mg 1 mL Tabless 100 611 0005 BG EVOLUTE CX 50 mg 3 mL Tabless 50 611 0010 BG EVOLUTE CX 100 mg 3 mL Tabless 50 611 0050 CG EVOLUTE CX 500 mg 6 mL Tabless 30 EVOLUTE AX 603 0002 AG EVOLUTE AX 25 mg 1 mL Tabless 100 613 0010 BG EVOLUTE AX 100 mg 3 mL Tabless 50 613 0050 CG EVOLUTE AX 500 mg 6 mL Tabless 30 ISOLUTE SLE Columns 820 0140 CG ISOLUTE SLE 1 mL sample volume Tabless 30 ISOLUTE SPE Columns ISOLUTE C18 220 0002 AG ISOLUTE C18 25 mg 1 mL Tabless 100 220 0010 BG ISOLUTE C18 100 mg 3 mL Tabless 50 220 0050 BG ISOLUTE C18 500 mg 3 mL Tabless 50 220 0100 CG ISOLUTE C18 1 g 6 mL Tabless 30 ISOLUTE SAX 500 0005 AG ISOLUTE SAX 50 mg 1mL Tabless 100 500 0050 BG ISOLUTE SAX 500 mg 3 mL Tabless 50 500 0100 CG ISOLUTE SAX 1 g 6 mL Tabless 30 ISOLUTE HCX 902 0002 A
128. ion Quantity 320 0025 P01 ISOLUTE 96 C2 25 mg plate 1 320 0050 P01 ISOLUTE 96 C2 50 mg plate 1 320 0100 P01 ISOLUTE 96 C2 100 mg plate 1 ISOLUTE Array C2 Loose Wells Part number Description Quantity 320 0025 R ISOLUTE Array C2 25 mg 1 mL wells 100 320 0050 R ISOLUTE Array C2 50 mg 1 mL wells 100 Pre assembled ISOLUTE Array plates are available To order add the suffix P to the equivalent loose well part number Support Documents TN101 Method Development in Solid Phase Extraction using Non polar ISOLUTE SPE Columns for the Extraction of Aqueous Samples TN112 General Approach to the Extraction of Basic Drugs from Biological Fluids using Non polar Non endcapped Sorbents TN126 Method Development in Solid Phase Extraction using Non polar ISOLUTE SPE Columns for the Extraction of Biological Fluid Samples See pages 158 and 159 for additional technical information about ISOLUTE C2 CA Aale eier fir E pm 2 m _ d Applications Matrix Analytes Retention Mechanism Si O Si CH CH Aqueous VVide polarity range Primary Non polar SI O SI CH Endcapped sorbent to minimize secondary silanol interactions Chemical structure of C2 silane and trimethyl silyl group covalently bonded to the surface of a silica particle Ordering Information ISOLUTE C2 EC SPE Columns Part number Description Quantity 321 0005 A ISOLUTE C2 EC 50 mg 1 mL 100 321 0010 A ISOLUTE C2 EC
129. ion of Aqueous Samples See pages 156 and 157 for additional technical information about ISOLUTE ENV Tabless columns for use with Pressure Positive Pressure Manifolds and other automated SPE systems Other tabless columns are available contact Biotage for details ISOLUTE 101 Polystyrene Divinylenzene Applications Matrix Analytes Retention Mechanism Aqueous Wide polarity range Primary Non polar Chemical structure of ISOLUTE 101 an unmodified polystyrene divinylbenzene co polymer Ordering Information ISOLUTE 101 SPE Columns Part number Description Quantity 101 0010 B ISOLUTE 101 100 mg 3 mL 50 101 0020 B ISOLUTE 101 200 mg 3 mL 50 101 0020 C ISOLUTE 101 200 mg 6 mL 30 101 0050 B ISOLUTE 101 500 mg 3 mL 50 101 0050 C ISOLUTE 101 500 mg 6 mL 30 Support Documents TN119 Method Development in Solid Phase Extraction using ISOLUTE 101 SPE Columns for the Extraction of Aqueous Samples See pages 156 and 157 for additional technical information about ISOLUTE 101 31 Chemical structure of C18 silane covalently bonded to the surface of a silica particle Ordering Information ISOLUTE C18 SPE Columns Part number 220 0002 A 220 0002 AG 220 0005 A 220 0005 G 220 0010 A 220 0010 B 220 0010 BG 220 0010 G 220 0020 B 220 0020 H 220 0050 B 220 0050 BG 220 0050 C 220 0050 H 220 0100 B 220 0100 C 220 0100 CG 220 0200 C 220 0200 D 220 0500 E 220 1000 F Description ISOLUTE C18 25 mg 1 mL ISOLUTE C18
130. ional clean up to remove interferences prior to analysis particularly for lovv level residue vvork This section highlights a range of products and techniques for simplifying sample handling and clean up of these difficult samples EVOLUTE SPE Columns EVOLUTE polymer based SPE products offer a simple robust generic approach to the isolation and clean up of small molecules from a variety of matrices including foodstuffs and water EVOLUTE ABN a hydrophobic hydrophilic balanced sorbent for simultaneous extraction and clean up of acidic basic and neutral analytes EVOLUTE CX a mixed mode strong cation exchange sorbent for selective extraction of basic analytes EVOLUTE CX columns have been shown to be produce significant results in the extraction of melamine from a variety of matrices including plasma and milk See www biotage com for the latest applications and posters EVOLUTE WCX a mixed mode weak cation exchange sorbent for selective extraction of basic analytes including quaternary amines using acidic elution conditions EVOLUTE AX a mixed mode strong anion exchange sorbent for selective extraction of acidic analytes EVOLUTE WAX a mixed mode weak anion exchange sorbent for selective extraction of acidic analytes including phosphates and sulphonic acids Ordering information for EVOLUTE products can be found on pages 21 27 ISOLUTE HM N Columns for Supported Liquid Extraction ISOLUTE HM N Supported Liquid Extraction columns
131. iption Column type Quantity Part number Bottom Luer cap fits all columns All 100 1201 0120 Top cap 1 mL column A 100 1201 0121 A Top cap 3 mL column B 100 1201 0122 B Top cap 6 mL column C 100 1201 0123 C Top cap 10 mL XL column G amp H 100 1201 0125 H Top cap 15 mL column D 100 1201 0126 D Top cap 25 mL column E 100 1201 0127 E Top cap 70 mL column F 100 1201 0128 F ISOLUTE Empty Reservoirs ISOLUTE empty reservoirs are available Ordering Information Description Quantity Part number ISOLUTE Reservoir 1 mL 100 120 1001 A ISOLUTE Reservoir 3 mL 100 120 1002 B ISOLUTE Reservoir 6 mL 100 120 1003 C ISOLUTE Reservoir 15 mL 100 120 1006 D ISOLUTE Reservoir 25 mL 100 120 1007 E ISOLUTE Reservoir 70 mL 50 120 1009 F 87 88 Accessorles For SPE and Filtration ISOLUTE Frits Biotage s filtration products are based on fritted systems consisting of sintered particles e g polyethylene PE which are compressed to produce a porous material The nominal porosity of the frit is the average or mean pore size e g 20 um Frit materials are relatively thick gt 1 mm and pores run through the material in a multi directional channel system For this reason a frit of nominal or mean pore size e g 20 um can retain a high percentage of particles smaller than 20 um in size Ordering Information Description Column Type Quantity Part number ISOLUTE Frit 6 mm diameter 20 um PE A 1 mL 100 120 1031
132. ired for an extraction it is essential to consider not only the capacity requirement for the analyte but also for any unde sired sample components interferences which may be co extracted with the analyte using the same retention mechanism Choosing a more selective retention mechanism will increase the relative capacity of the SPE bed in effect reducing the amount of sorbent required for a given extraction This has benefits in reducing the amounts of solvent used and increasing the final concentration of analyte in the extract Maximum selectivity and therefore capacity is achieved when the selected sorbent interacts with functional groups present on the analyte but not other sample components Typically non polar and polar SPE sorbents have a capacity of between 1 and 5 of the sorbent mass i e 100 mg of sorbent can retain up to 5 mg of strongly retained analyte under optimum conditions Ion exchange sorbent capacity is measured in milli equivalents per gram of sorbent meq g based on the num ber of available ionic groups on the sorbent For example ISOLUTE SAX has an exchange capacity of 0 6 meq g This means a 1 g ISOLUTE SAX column can retain up to 0 6 mmol of an anionic acidic compound Guide to SPE Sorbent Selection Proper choice of SPE sorbent is critical to the success of the SPE procedure There are many aspects of the appli cation that should be considered as part of sorbent selection including e Nature of the
133. ix to another e g from aqueous to organic solvent e Desalting e Derivatization analytes are retained on a sorbent derivatized then eluted e Class fractionation sample is separated into different compound groups that share common properties SPE offers many benefits and advantages over other sample preparation techniques such as liquid liquid extraction including e High reproducible analyte recovery e Concentration of analytes e Highly purified extracts e Ease of automation e Compatibility with instrumental analysis e Productivity enhancement e Reduction of organic solvent consumption SPE is a very simple technique to use employing disposable extraction columns or microplates see Figure 1 which are available in a wide range of reservoir volumes formats and sorbents as described on pages 15 63 of this catalog Reservoir Sorbent bed Frits Polyethylene stainless steel or teflon Luer tp Figure 1 Components of an SPE column or vvell microplate format 174 QuickStart Guide to SPE In principle SPE is analogous to liquid liquid extraction LLE As a liquid sample passes through the SPE column compounds are extracted from the sample and adsorbed onto the support or sorbent material in the column Interferences can then be selectively removed from the column using the correct choice of wash or interference elution solvents Finally the desired analytes may be se
134. ixed Well Plates Part number Description Quantity 533 0025 PO1 ISOLUTE 96 SCX 3 25 mg plate 1 533 0050 PO1 ISOLUTE 96 SCX 3 50 mg plate 1 533 0100 PO1 ISOLUTE 96 SCX 3 100 mg plate 1 ISOLUTE Array SCX 3 Loose Wells Part number Description Quantity 533 0025 R ISOLUTE Array SCX 3 25 mg 1 mL wells 100 533 0050 R ISOLUTE Array SCX 3 50 mg 1 mL wells 100 533 0100 T ISOLUTE Array SCX 3 100 mg 2 mL wells 100 Pre assembled ISOLUTE Array plates are available To order add the suffix P to the equivalent loose well part number Support Documents TN106 Method Development in Solid Phase Extraction using ISOLUTE SCX and SCX 3 SPE Columns for the Extraction of Aqueous Samples See pages 166 and 167 for additional technical information about ISOLUTE SCX 3 5 SI SILICA Chemical structure of silanol groups on the surface of a silica particle Ordering Information ISOLUTE SI SPE Columns Part number Description 460 0002 A ISOLUTE SI 25 mg 1 mL 460 0010 A ISOLUTE SI 100 mg 1 mL 460 0010 B ISOLUTE SI 100 mg 3 mL 460 0010 G ISOLUTE SI 100 mg 10 mL 460 0020 B ISOLUTE SI 200 mg 3 mL 460 0020 H ISOLUTE SI 200 mg 10 mL 460 0050 B ISOLUTE SI 500 mg 3 mL 460 0050 C ISOLUTE SI 500 mg 6 mL 460 0050 H ISOLUTE SI 500 mg 10 mL 460 0100 B ISOLUTE SI 1 g 3 mL 460 0100 C ISOLUTE SI 1 g 6 mL 460 0200 C ISOLUTE SI 2 g 6 mL 460 0200 D ISOLUTE SI 2 g 15 mL 460 0500 E ISOLUTE SI 5 g 25 mL 460 1000 F ISOLUTE SI 10 g 70 mL Support Doc
135. le Preparation Format Options Sample Prep Format Options True to Scale Columns All the SPE column and reservoir size options are illustrated here as true to scale drawings The last character of the ISOLUTE or EVOLUTE SPE column or empty reservoir part number identifies the column reservoir size For example the 100 mg C18 ISOLUTE column P N 220 0010 A uses column A whereas the 100 mg C18 ISOLUTE XL SPE column P N 220 0010 G contains the same sorbent and sorbent mass packed in column G The nominal volume listed for each reservoir is for the reservoir without sorbent Tabless columns 1 3 and 6 mL for use on PRESSURE manifolds see pages 94 96 are also available ISOLUTE Array Sample Prep Format Options True to Scale 96 vvell plate Fixed vvell and Array plate Biotage supplies tvvo 96 vvell plate formats the fixed vvell plate and the modular Array As vvith individual columns the last character s of the part number identifies the format column type The suffix for fixed vvell plates is P01 vvhile the suffix for Array vvells is R 1 mL and T 2 mL Prepacked Array plates have the suffixes RP and TP Scale diagrams 60 of actual size of the fixed well and pre assembled Array plate are shown below Note the skirt heights distance from well outlet to sealing edge of the two plates are different To eliminate cross talk when processing plates well outlets should penetrate the co
136. le pH Non polar polymer based sorbents see page 22 27 30 31 156 159 EVOLUTE ABN Methanol Aqueous formic Dilute with Water methanol Methanol acid 0 1 v v aqueous formic 95 5 v v acid 1 1 3 v v pH control may be required to suppress ionization of acidic and basic analytes ISOLUTE ENV Methanol or Water and or 20 pH control Water or buffer Methanol Evaluate Hydroxylated acetonitrile 50 mM buffer the required to add up to 40 other solvents e g polystyrene although not same pH as the suppress methanol or hexane acetone divinylbenzene necessary sample ionization of acetonitrile to ethyl acetate etc if water wettable acidic and basic remove more polar additional selectivity is sorbent compounds interferences required Addition of ensure no analyte acid or base may be breakthrough necessary to break Maintain pH ionic secondary control interactions ISOLUTE 101 Methanol or Water and or 20 pH control Water or buffer Methanol Evaluate Unmodified acetonitrile 50 mM buffer the required to add up to 40 other solvents e g polystyrene same pH as the suppress methanol or hexane acetone divinylbenzene sample ionization of acetonitrile to ethyl acetate etc if non wettable acidic and basic remove more polar additional selectivity is sorbent compounds interferences required ensure no analyte breakthrough Maintain pH control If using a water immiscible elution solv
137. lease contact your local Biotage representative or Distributor for details of our Custom Manufacturing Service see page 187 189 ISOLUTE Bulk Sorbents ISOLUTE HM N a modified form of diatomaceous earth is also available in bulk The material is used for applications requiring an inert Support as part of the sample preparation process e g SFE For details of pre packed columns containing ISOLUTE HM N see page 79 Ordering Information Inert Support modified diatomaceous earth Part number Description Quantity 9800 1000 ISOLUTE HM N bulk 1 Kg 9800 5000 ISOLUTE HM N bulk 5 Kg ISOLUTE HM N is available in additional quantities to those detailed in the above table Please contact your local Biotage representative or Distributor for details of our Custom Manufacturing Service see page 187 189 67 Er R u FE z EN FRUIT ve r al TABLE SOLUTE ZuEChERS Bi KORG WAXED FRUIT SNES ISOLUTE QuEChERS f l LL gt E ey 4 5 sal d gt N gt y Y A 1 a 4 N A 5 70 QuEChERS ISOLUTE QuEChERS Fast and efficient clean up of complex samples QuEChERS pronounced catchers stands for Quick Easy Cheap Effective Rugged and Safe The technique is designed to be simple and involve a minimum number of steps but still allow for effective clean up of complex samples T
138. lectively recovered from the column by an elution solvent resulting in a highly purified extract The analyte concentration in this extract is often higher than in the original sample Alternatively an extraction column may be selected that retains the interferences present in the sample but allovvs the analytes to pass through un retained providing clean up but not analyte trace enrichment SPE sorbents have a typical mean particle size of 30 50 um Many organic solvents can flow through SPE columns or plates under gravity but for aqueous samples and more viscous solvents liquids must be passed through the sorbent bed using vacuum applied to the column outlet positive pressure applied to the column inlet or centrifugation see Figure 2 N 7 7 1 Centrifugation Vacuum Manifold Positive Pressure Manual Figure 2 Techniques for processing ISOLUTE SPE columns SPE Sorbent Mechanism Selection SPE columns are available in a wide range of sorbent chemistries Each exhibits unique properties for retention of analytes through a variety of molecular interactions referred to as retention mechanisms The most common retention mechanisms in SPE are e Non polar based on Van der Waals forces e Polar based on hydrogen bonding dipole dipole or pi pi interactions e 1on exchange interactions between cations positively charged species and anions negatively charged species e Mixed mode combination o
139. lephone 46 18 56 59 11 E mail EU 1 pointsupport biotage com United States Service and Support Telephone 1 800 446 4752 Outside the USA 1 704 654 4900 E mail US 1 pointsupport biotage com Japan Service and Support Telephone 81 3 5627 3123 E mail JP 1 pointsupport biotage com China Service and Support Telephone 86 21 2898 6655 E mail CN 1 pointsupport biotage com To locate a distributor please visit our vveb site at VVVVVV biotage com How to Place Your Order Product information is displayed with easy to follow application details and supporting documentation Ordering information is found following each product listing How to Order United States and Canada Distributors Main Office 1 704 654 4900 Please visit our Web site at Toll Free 1 800 446 4752 www biotage com for contact details Fax 1 704 654 4917 Order Tel 1 704 654 4900 Order Fax 1 434 296 8217 ordermailbox biotage com Europe Main Office 46 18 56 59 00 Toll Free 800 18 56 57 10 Fax 46 18 59 19 22 Order Tel 46 18 56 57 10 Order Fax 46 18 56 57 05 order biotage com Japan Tel 81 3 5627 3123 Fax 81 3 5627 3121 jp_order biotage com China Tel 86 21 2898 6655 Fax 86 21 2898 6153 CN_order biotage com www biotage cn When placing your order please have available e Your purchase order number e Biotage part number s e Product description s e Shipping address e Billing address e Contact perso
140. les ISOLUTE ENV is a hydroxylated polystyrene divinylbenzene polymer that can extract very polar compounds from a variety of aqueous or partially aqueous sample matrices The high capacity polymer retains polar drugs and metabolites from biological fluid samples and polar pesticides and herbicides from environmental matrices Application Chemistry Ordering Data Sheets Information Page TN131 22 27 TN138 Extraction of acidic neutral and basic drugs from biological fluids using generic method Extraction of acidic neutral and basic compounds from aqueous samples using generic method Extraction of very polar compounds that are not retained by C8 and C18 non polar silica TN109 based sorbents TN119 OJ Extraction of polar compounds from aqueous sample matrices W UJ N Most commonly used C18 sorbent for the extraction of acidic neutral and basic TN101 112 amp compounds from aqueous matrices Secondary silanol or ionic interactions can be used to 126 enhance extract purity and method robustness for basic compounds Extraction from aqueous matrix using non polar interactions for acidic neutral and basic TN101 compounds Extraction from aqueous matrix using both non polar interactions for acidic neutral and TN101 amp 112 3 basic compounds and readily accessible secondary silanol or ionic interactions for basic compounds only Not the ideal choice when working at extreme pH C18 is the prefer
141. llection plate correctly To accommodate this skirt height difference the VacMaster 96 is supplied complete with a spacer for use with the Array format This spacer is not required for processing the fixed well format Total height 2 mL T 67 mm Side elevation 60 of actual size of populated Array plate top and fixed well plate bottom AFFINILUTE MIP AFFINILUTE Molecularly Imprinted Polymers AFFINILUTE MIP Biotage AFFINILUTE MIP products offer analytical chemists ultimate selectivity compared with standard solid phase extraction techniques Unlike other methods Biotage AFFINILUTE MIP products rely on the specific molecular structure of the analyte for targeted capture resulting in exceptional clean up Biotage AFFINILUTE MIP columns combine high affinity with standard flow through sample preparation methodology AFFINILUTE MIP columns contain Molecularly Imprinted Polymers MIPs which are a class of highly cross linked polymers engineered to bind one target compound or a class of structurally related target compounds with high selectivity This selectivity is introduced during MIP synthesis in which a template molecule designed to mimic the analyte guides the formation of specific cavities or imprints that are sterically and chemically complementary to the target analyte s MIPs are prepared by first
142. lyte Through these experiments there was no universal best solvent for elution discovered While MTBE is widely used ethyl acetate DCM and mixed solvents also perform well Depending on the drug s functionality by simply screening two pHs combined with 4 extraction solvents you can develop a method in minutes ISOLUTE SLE Supported Liquid Extraction Plates Method Selection Buffar 1 l Buffer 2 Buffer 3 Buffer 4 Adjust plasma to pH 3 4 plasma lo 6 3 Adjuet plasma te pH 10 Adjust pisama 45 pH 10 4 Mis pama wet L ye Mir plasma weh OL ity Mis piena wah water 1 1 wir Mix piema rif C1 M dunun Perit Achi DE wY dursun Forme 404 111 ity Ammonia Hydr rid 111 ww Extraction Solvent 1 Extraction Solvent 2 Extraction Solvent 3 Extraction Solvent 4 Tri tee ee gode h da na in Fei Be ER er l ei Me Suerte Lea Eran eed Te belie bad er en Pie ge G pHi ee Gee fee OA PET A eet Dr of A A asd hu welled Thee eed oe eed bi EA Pe A H zi MAD Latin hte hit Fi E A Ee ee a de very EHE Bee LST Fe ite BA Coe Mp ba Ri B ee fect Gare ur hz Bae far A FERIA irin Mecs lar Re poli A e ne k ed H ee a ee Fi LE AAA anaya 0 B 4 Lulu Feige F dido a sad azala q a a a arte ea LAA as el x r rere un I 70 IO d e HER PT PA PA A A a Li bee APS a PARTO A APNEA A 121 i NOTE A full sized copy of the SLE Method Selection Guide can be downloaded from our website at www
143. mL 10 mL 15 mL 25 mL 70 mL o glass 6 mL e 96 well plate type o fixed well plate o Array format wells 1 mL 2 mL o Array format plate 1 mL 2 mL e Sorbent type e Bed mass e Frittype material and porosity e Quantity required e Any special packaging requirements we can supply bulk packs Sample processing equipment and accessories can also be supplied under our Custom Manufacturing Service 189 Index 96 well accessories 90 96 well filtration plates 90 101 Polystyrene divinylbenzene SPE columns 31 129 156 157 A Accessories SPE and filtration 86 91 Adaptors polyethylene for SPE columns 86 Adaptors PTFE for SPE columns 86 AFFINILUTE MIP 15 19 120 127 137 147 Agrochemical analysis 146 151 AL Alumina SPE columns 63 170 171 Aminopropyl see NH2 Applications Bioanalytical 123 Clinical 132 133 Environmental 142 Forensic 130 131 Food 6 Agro 151 Aromatic sulfonic acid see SCX SCX 3 Array SPE vvells and 96 vvell plates 13 91 ASPEC cap fitting service 188 Automated Sample Processing Products 105 109 Benzenesulfonic acid see SCX SCX 3 Bioanalytical sample preparation products 118 123 Bulk sorbents ISOLUTE 66 67 Butyl see C4 C C2 C2 EC Ethyl 96 well SPE plates 39 40 158 159 Bulk sorbent 66 SPE columns 39 40 158 159 C4 Butyl 96 well SPE plates 38 158 159 SPE columns 38 158 159 C6 Hexyl SPE columns 37 156 157 96 well SPE plates 37 156 157
144. matrices The result is it mu cleaner extracts compared to other leading polymer based SPE products EVOLUTE ABN a hydrophobic hydrophilic balanced sorbent for simultaneous extraction and clean up of acidic basic and neutral analytes EVOLUTE CX a mixed mode strong cation exchange sorbent for selective extraction of basic analytes EVOLUTE VVCX a mixed mode vveak cation exchange sorbent for selective extraction of basic analytes including quaternary amines using acidic elution conditions EVOLUTE AX a mixed mode strong anion exchange sorbent for selective extraction of acidic analytes EVOLUTE VVAX a mixed mode vveak anion exchange sorbent for selective extraction of acidic analytes including phosphates and sulphonic acids See page 21 27 for more details and ordering information Application notes for EVOLUTE products are available through www biotage com applications Bioanalytical Applications and Products for Bioanalysis ISOLUTE ENV ISOLUTE ENV SPE columns and plates are ideal for extraction of extremely polar compounds which are not retained on EVOLUTE ABN The sorbent is suitable for extraction of polar acidic basic and neutral drugs and metabolites from biological fluids See page 30 for more details and ordering information ISOLUTE Non polar SPE Products Available in a range of column and plate formats ISOLUTE non polar sorbents offer a range of choices for the extraction of aci
145. mixing a template molecule that consists of a structural analog of the analyte s of interest with one or more functional monomers The monomers form spontaneous complexes around the template Upon complex formation cross linking monomers are then added with a suitable porogen solvent that aids in the role in pore formation to drive polymerization An extensive wash procedure is used to remove the template from the polymer leaving imprints or binding sites that are sterically and chemically complementary to the template The very specific selectivity designed into each AFFINILUTE MIP ensures high affinity binding even of rare analytes in large sample volumes thus lowering limits of detection Funcional Temple L Selectiva Inter action Achieve lower detection limits through superior selectivity Minimize ion suppression AFFINILUTE MIPs are so selective that stringent wash steps can be applied prior to analyte elution in order to remove all interferences and thus minimize matrix effects Minimal to no method development required Each box of AFFINILUTE MIP product contains a detailed application note method card that in most cases will require no further optimization as Biotage have optimized the method during polymer development AFFINILUTE Molecularly Imprinted Polymers Stable at broad pH ranges and high temperatures AFFINILUTE MTPs are highly cross linked polymers that can withstand extremes of p
146. mns is available Based on extraction of 5 mL urine samples high recoveries gt 90 of the main DOA classes are ensured Extracts are suitable for TLC screening and GC MS confirmation at the NIDA specified cut off levels Application Notes for ISOLUTE HCX columns Amphetamine and methamphetamine Cocaine and benzoylecgonine Opiates codeine and morphine IST1050 LS IST1041 Phencyclidine IST1039 Fractionation of basic neutral and acidic drugs of abuse IST1044 All Application notes are available to download from www biotage com applications Simplified Single Column Methodology for Automated Drugs of Abuse Testing Based on the robust chemistry of ISOLUTE HCX Biotage has developed a simple single column approach that can be used for the extraction of all NIDA 5 SAMHSA 5 drugs of abuse classes The SPE methodology designed for use with the Biotage RapidTrace uses only eight solvents in total Request Application Note IST1081 for more information Forensic and Clinical Applications and Products for Forensic and Clinical Analysis Toxicological Screening Using ISOLUTE 101 SPE Columns ISOLUTE 101 is a non selective polymeric sorbent used in toxicological screening for a wide range of drug classes from biological fluids The 500 mg 6 mL column configuration part number 101 0050 C has been successfully applied to screening more complex samples such as post mortem blood and tissue ref Stimpfl et al J Anal Toxicol 20
147. mplete elution elution complete elution complete elution elution Evaporate to Evaporate to Evaporate to dryness Evaporate to Evaporate to Evaporate to dryness dryness and dryness and and reconstitute in dryness and dryness and and reconstitute in reconstitute in reconstitute in mobile phase prior to reconstitute in reconstitute in mobile phase prior to mobile phase prior to analysis analysis mobile phase prior mobile phase prior analysis mobile phase prior E to analysis to analysis to analysis NOTES 1 Sample Pre treatment Dilute sample 1 1 with buffer depending on nature of analyte a For highly acidic analytes dilute sample 1 1 with 1 aqueous formic acid to pH 3 2 b For neutral to mildly acidic analytes dilute sample 1 1 with 0 1 aqueous formic acid to pH 6 3 c For neutral to mildly basic analytes dilute sample 1 1 with water to pH8 0 d For strongly basic analytes dilute sample with 0 5M ammonium hydroxide to pH 10 4 2 Viscous samples may require additional dilution 3 Analyte Elution Organic solvents MTBE DCM DCM IPA 95 5 v v and ethyl acetate are all suitable as elution solvents depending on the nature of the anlayte 185 Custom Manufacturing Service 188 Custom Manufacturing Service Biotage supply a comprehensive range of products for bioanalytical environmental forensic clinical food and agrochemical sample preparation many of which are listed in this catalog However we know
148. n including telephone number e Product user name and department Orders can also be placed using your VISA or MasterCard account and American Express IN THE US ONLY VISA DE MasterCard unununun ian Prices and Specifications Prices in the Biotage catalog are suggested list prices and are current at the time of the book s printing and are subject to change without notice Specifications not listed in this catalog can be obtained by contacting Biotage Customer Service or your local distributor Product Quality Biotage is committed to developing and manufacturing sample preparation products of the highest quality State of the art manufacturing techniques are supported by a comprehensive Quality Control QC testing program documented under our 1509001 2008 registered Quality Management System All the components used to make our sample preparation products tubes frits 96 vvell plates and SPE sorbents are rigorously cleaned and QC tested to ensure they meet our demanding purity specifications We use sophisticated instrumental techniques to confirm the physical and chemical nature of every batch of SPE sorbent ensuring reproducible performance in your application Every ISOLUTE or EVOLUTE SPE product is accompanied by a detailed Quality Assurance QA report for your reference This page explains the importance of the information it contains and the impact this has on every sample preparation procedure you perform
149. n polar interferences Maintain the same pH as the sample and evaluate the addition of 20 50 methanol to elute non polar interferences Analyte Elution Semi polar solvent mixture e g hexane ethyl acetate For a non selective elution use a polar solvent such as methanol Elute with buffer at least 2 pH units above the pK of the basic analyte Alternatively use high ionic strength buffer gt 0 1 M Elution with methanol containing 2 5 ammonia or other volatile base is common Elute with buffer at least 2 pH units above the pK of the basic analyte This should contain at least 25 50 organic solvent to overcome secondary interactions Alternatively use high ionic strength buffer gt 0 1 M Elution with methanol containing 2 5 ammonia or other volatile base is common Buffer at pH lt 2 8 will to eliminate the charge on the sorbent Alternatively use high ionic strength buffer gt 0 1M or acidi fied solvent Elute with buffer at least 2 pH units below the pK of the acidic analyte Alternatively use high ionic strength buffer gt 0 1M or acidified methanol Elute with buffer at least 2 pH units below the pK of the acidic analyte Evaluate addition of 10 methanol to reduce elution volume Alternatively use high ionic strength buffer gt 0 1M or acidified methanol Elute with buffer or methanol at pH gt 11 8 to eliminate the charge on the sorbent Alternatively
150. nks can be processed using the same vacuum source connected with the PTFE T valve see page 98 All compo nents are easy to clean Vacuum safety Each system is fully tested under a strict vacuum cycling regime to ensure safe operation A vacuum safety valve is fitted as standard to prevent safe vacuum levels being exceeded VacMaster 10 amp 20 Sample Processing Manifolds Vacuum Options VacMaster manifolds should be used in conjunction with an external vacuum source vacuum pump or the VacMaster VCU 2 unit see page 99 In either case a solvent trap see page 101 should be placed between the manifold and vacuum source Ordering Information VacMaster 10 Sample Processing Manifold Description Quantity Part number VacMaster 10 Sample Processing Manifold complete with 1 121 1016 16 mm collection rack and fitted with stainless steel needles as option 4 below Other collection rack sizes can be supplied through our Custom Manufacturing Service Maximum hole diameter 27 mm VacMaster 20 Sample Processing Manifold Description Quantity Part number VacMaster 20 Sample Processing Manifold complete with 1 121 2016 16 mm collection rack and fitted with stainless steel needles as option 4 belovv Other collection rack sizes can be supplied through our Custom Manufacturing Service Maximum hole diameter 23 mm PTFE Stopcock Option and Spare Parts Description Quantity Part number Universal PTFE stopcock 1
151. ntity 390 0025 P01 ISOLUTE 96 C4 25 mg plate 1 ISOLUTE Array C4 Loose Wells Part number Description Quantity 390 0025 R ISOLUTE Array C4 25 mg 1 mL wells 100 Pre assembled ISOLUTE Array plates are available To order add the suffix P to the equivalent loose well part number Support Documents TN101 Method Development in Solid Phase Extraction using Non polar ISOLUTE SPE Columns for the Extraction of Aqueous Samples TN112 General Approach to the Extraction of Basic Drugs from Biological Fluids using Non polar Non endcapped Sorbents See pages 158 and 159 for additional technical information about ISOLUTE C4 SOL UTE C9 I Y m Z Applications Matrix Analytes Retention Mechanisms Primary Non polar Aqueous Wide polarity range 5 0 Secondary Polar and vveak cation exchange SI OH Chemical structure of C2 silane covalently bonded to the surface of a silica particle Ordering Information ISOLUTE C2 SPE Columns Part number Description Quantity 320 0002 A ISOLUTE C2 25 mg 1 mL 100 320 0005 A ISOLUTE C2 50 mg 1 mL 100 320 0010 A ISOLUTE C2 100 mg 1 mL 100 320 0010 B ISOLUTE C2 100 mg 3 mL 50 320 0010 G ISOLUTE C2 100 mg 10 mL 50 320 0020 B ISOLUTE C2 200 mg 3 mL 50 320 0050 B ISOLUTE C2 500 mg 3 mL 50 320 0050 C ISOLUTE C2 500 mg 6 mL 30 320 0100 C ISOLUTE C2 1 g 6 mL 30 320 0200 D ISOLUTE C2 2 g 15 mL 20 ISOLUTE 96 C2 Fixed Well Plates Part number Descript
152. olumn for direct sample loading and in line removal of suspended solids For samples containing higher levels of suspended solids ISOLUTE Depth Filter Reservoirs should be used Ordering information for ISOLUTE Filtration Columns can be found on page 88 Ordering information for ISOLUTE Depth Filter Reservoirs can be found on page 86 Agrochemical and Food Applications and Products for Agrochemical and Food Analysis ISOLUTE Sodium Sulfate Drying Cartridges Prior to analyte derivatization or analysis it is often necessary to dry SPE elution solvents using loose sodium sulfate ISOLUTE Sodium Sulfate Drying Cartridges can be connected to the outlet of the SPE columns for in line solvent drying eliminating this time consuming step Ordering information for ISOLUTE Sodium Sulfate Drying Cartridges can be found on page 89 Custom Manufacturing Options Your application may require alternative product configurations to those listed in this section Through our Custom Manufacturing Service we can supply alternative configurations including e SPE columns fitted with stainless steel frits for additional extract purity e Glass SPE columns with PTFE frits e SPE columns fitted with integral depth filters Please see pages 187 189 for details of our Custom Manufacturing Service For up to date application notes please check www biotage com applications Agrochemical and Food Applications and Products for Agrochemic
153. on of some acidic analytes than SAX Sorbent Class Exchange Application Chemistry Ordering Capacity Information meq g Page VVeak anion Extraction of strong acids and polyacidic compounds from exchanger pK aqueous sample matrix Analyte elution can be performed by 9 8 neutralizing charge on the sorbent Sorbent supplied as the free base VVeak anion Extraction of strong acids and polyacidic compounds from exchanger pK aqueous sample matrix Analyte elution can be performed by 10 1 amp 10 9 neutralizing charge on the sorbent Sorbent vvill complex vvith certain metal ions Supplied as the free base Strong anion Extraction of acidic analytes from aqueous sample matrix exchanger Supplied vvith chloride counter ion Strong anion Extraction of acidic analytes from aqueous sample matrix exchanger Supplied vvith acetate counter ion for more efficient extraction of acidic analytes including those vvith polar vvater soluble character istics For more information on ion exchange SPE procedures see the QuickStart Guide page 174 184 For in depth technical information on the use of ISOLUTE ion exchange sorbents refer to the Chemistry Data Sheets listed in the table above 165 166 lon Exchange Sorbents ISOLUTE Cation Exchange Sorbents ISOLUTE cation exchange sorbents are used for extraction of basic compounds The vveak cation exchange sorbent ISOLUTE CBA is suitable for the extraction of strongly bas
154. ontact Biotage for details Tabless and standard SPE columns Ordering information for high throughput formats can be found on the relevant sorbent page RapidTraces SPE Workstation The RapidTrace SPE Workstation is a modular highly scalable automated sample preparation platform designed for high throughput Units are available to accommodate 1 mL 3 mL or 6 mL industry standard SPE cartridges and can process up to 100 samples in less than two hours Analysis of samples can then be effectively performed using either LC MS MS or GC MS Application note AN 1081A Extraction of NIDA 5 SAMHSA 5 Analytes using RapidTrace as well as many other applications are available to download from www biotage com applications Applications and Products for Bioanalysis Bioanalytical Applications Application number IST1044A AN702 AN707 AN603 AN721 AN722 AN731 AN601 AN726 AN734 Analyte Acidic Neutral and Basic Drugs Basic Drugs B Blockers Non steroidal Anti inflammatory Drugs NSAIDs Tamoxifen Tamoxifen Therapeutic and Illicit Drugs of Abuse Tricyclic Antidepressants Tricyclic Antidepressants Warfarin Matrix Urine Plasma Biological Fluids Plasma Urine Plasma Biological Fluids Plasma Whole Blood Plasma Bioanalytical Format ISOLUTE HCX 130 mg 10 mL column EVOLUTE CX 25 mg Array fixed well plate AFFINILUTE MIP 25 mg 3 mL or
155. ood Analysis Technical Section QuickStart Guide Custom Manufacturing Service Index Part Number Index 11 15 21 29 65 69 73 81 85 93 105 117 125 135 145 153 173 187 190 193 Nevv Products Biotage PRESSURE 48 and 96 Positive Pressure Manifolds Biotage PRESSURE manifolds deliver positive pressure parallel processing for 96 well plates 1 mL 3 mL and 6 mL column formats The systems utilize a consistent uniform flow of positive pressure to move both lovv and high viscosity liquids through Solid Phase Extraction Supported Liquid Extraction and Protein Precipitation plates and columns This precise control of applied pressure increases reproducibility of analyte recoveries and ensures consistent extraction efficiencies especially vvhen compared vvith vacuum controlled processing See page 94 RapidTraceo Automated SPE Workstation The RapidTrace SPE Workstation has been updated to include new features These include the ability to process larger sample volumes of up to 40 mL a new turret system allowing for 1 mL 3 mL and 6 mL SPE columns to be used on the same unit and the ability to use a broader range of column bed sizes See page 106 TurboVap Concentration VVorkstations for Sample Evaporation Biotage offers a range of TurboVap Concentration VVorkstations for sample evaporation TurboVap units use a patented vortex evaporation system that together vvith a vvater bath for
156. ous Samples See pages 164 and 165 for additional technical information about ISOLUTE PSA m pm r A 4 l l 2 A x zi or Applications Matrix 0 Analytes Retention Mechanisms Aqueous Primary Strong anion exchange Anionic Secondary Non polar Non polar Primary Strong anion exchange SI OH Chemical structure of SAX silane covalently bonded to the surface of a silica particle Ordering Information ISOLUTE SAX SPE Columns Part number Description Quantity 500 0002 A ISOLUTE SAX 25 mg 1 mL 100 500 0005 A ISOLUTE SAX 50 mg 1 mL 100 500 0005 AG ISOLUTE SAX 50 mg 1 mL Tabless 100 500 0005 G ISOLUTE SAX 50 mg 10 mL 50 500 0010 A ISOLUTE SAX 100 mg 1 mL 100 500 0010 B ISOLUTE SAX 100 mg 3 mL 50 500 0010 C ISOLUTE SAX 100 mg 6 mL 30 500 0010 G ISOLUTE SAX 100 mg 10 mL 50 500 0020 B ISOLUTE SAX 200 mg 3 mL 50 500 0020 H ISOLUTE SAX 200 mg 10 mL 50 500 0050 B ISOLUTE SAX 500 mg 3 mL 50 500 0050 BG ISOLUTE SAX 500 mg 3 mL Tabless 50 500 0050 C ISOLUTE SAX 500 mg 6 mL 30 500 0050 H ISOLUTE SAX 500 mg 10 mL 50 500 0100 B ISOLUTE SAX 1 g 3 mL 50 500 0100 C ISOLUTE SAX 1 g 6 mL 30 500 0100 CG ISOLUTE SAX 1 g 6 mL Tabless 30 500 0200 D ISOLUTE SAX 2 g 15 mL 20 ISOLUTE 96 SAX Fixed Well Plates Part number Description Quantity 500 0025 P01 ISOLUTE 96 SAX 25 mg plate 1 500 0050 P01 ISOLUTE 96 SAX 50 mg plate 1 500 0100 P01 ISOLUTE 96 SAX 100 mg plate 1 ISOLUTE Array S
157. ow well collection plates 1 121 9615 VacMaster 96 insert 12 mm for shallow skirt plates o 1 121 9611 100 Sample Processing Accessories VacMaster Trap Kit The VacMaster Trap kit should be installed betvveen the outlet of the sample processing manifold and the vacuum source trapping all liquids aspirated through the manifold The trap is compatible vvith VacMaster 10 20 and 96 Sample Processing Manifolds The kit consists of a 1L polypropylene vacuum flask polyethylene stopper vvith connectors sealing gasket and 1 5 m vacuum tubing Features and Advantages e Collect waste solvents and samples cleanly and efficiently e Prevent contamination of vacuum control units removing spray of aspirated liquid e Easy to clean components reducing preparation time Ordering Information Description Quantity Part number VacMaster Trap Kit complete 1 121 2095 VacMaster Drying Adapter Prior to elution of analytes from an SPE column with water immiscible solvents it is often neces sary to dry the sorbent bed to achieve optimum and reproducible recoveries The VacMaster Drying Adapter directs a stream of gas efficiently through the column significantly reducing drying times compared to vacuum aspirated air flow The adapter comes complete with all fittings for 1 3 and 6 mL columns Features and Advantages e Can be used with standard laboratory gas systems e g nitrogen air e Fast efficient SPE col
158. own that most food intake of PAHs comes from cereals oils and fats The application note for AFFINILUTE MIP PAH describes the extraction of nine of the most toxic PAHs from olive oil fluoranthene benzo a anthracene chrysene benzo b fluoranthene benzo k fluoranthene benzo a pyrene indeno 123 cd pyrene dibenzo ah anthracene and benzo ghi perylene Description Quantity Part number AFFINILUTE MIP PAH 25 mg 3 mL 50 M73 0002 B AFFINILUTE MIP NSAIDs Nonsteroidal anti inflammatory drugs NSAIDs are analgesic and antipyretic fever reducing agents which at higher doses also have anti inflammatory effects The most common members of this group of drugs are aspirin ibuprofen and naproxen which are widely available as over the counter drugs in many areas This widespread availability can lead to toxicity both intentional and unintentional hence accurate extraction and analysis is often required in clinical and forensic testing laboratories Biotage have developed an application note for AFFINILUTE MIP NSAIDs that describes the extraction of NSAIDs from solid tissue as well as liquid samples Description Quantity Part number AFFINILUTE MIP NSAIDs 25 mg 3 mL 50 M72 0002 B AFFINILUTE MIP NSAIDs 25 mg 10 mL 50 M72 0002 G AFFINILUTE Molecularly Imprinted Polymers AFFINILUTE MIP Fluoroquinolones Fluoroquinolones are a family of synthetic broad spectrum veterinary antibiotics which are on the US EPA list for required testing of all imported
159. pK of the analyte ii Use of non polar non endcapped silica based sorbents C18 C8 C6 C4 C2 PH CN for extraction of basic analytes The retention of basic analytes by non polar sorbents can often be enhanced using the secondary interaction with silanol Si O groups present on the surface of the silica particle To use this approach the pH of the sample should be adjusted to ionize the silanol groups Si O and protonate the analyte A pH range between 3 and 8 is normally evaluated QuickStart Guide to SPE d For ion exchange interactions pH must be adjusted to ensure complete ionization of the analyte and in the case of weak ion exchange sorbents e g CBA NH2 and PSA the sorbent functional group Analyte ionization conditions i For basic analytes adjust pH to 2 pH units below the pK of the analyte ii For acidic analytes adjust pH to 2 pH units above the pK of the analyte Sorbent ionization conditions i For ISOLUTE CBA sample pH must be greater than 6 8 ii For ISOLUTE NH2 sample pH must be less than 7 8 or 8 1 for ISOLUTE PSA It is important to ensure that the ionic strength of the sample is not so high as to weaken the ionic interac tions To overcome this dilute with low ionic strength buffer Failure to deal with high and variable ionic strength Samples can result in low or variable recoveries when using ion exchange sorbents e For extraction of large volume aqueous samples gt 25 mL using non polar sil
160. plate 1 x 2 mL 96 well collection plate 1 x 10 mL 24 well collection plate waste plate 96 column sealing gasket gas supply adapter kit 6 of 1 8 i d polyethylene tubing and 1 8 and 1 4 NPT connectors User Manual CD ROM Accessories PPM A96 GSKT PRESSURE 96 Sealing Gasket 96 position PPM A96 1024 PRESSURE 96 Collection Tray 10 mL 24 well PPM GA PRESSURE Gas Supply Adapter for all models 121 5202 96 well collection plate 1 mL pack of 50 121 5203 96 well collection plate 2 mL pack of 50 PRESSURE 48 Part number Description PPM 48 PRESSURE 48 Positive Pressure Manifold 48 position Includes Sealing gasket 48 position gas supply adapter kit 6 of 1 8 i d polyethylene tubing and 1 8 and 1 4 NPT connectors waste bin rack waste bin inserts User Manual CD ROM Racks must be ordered separately Racks PPM A48 1RCK PPM A48 3RCK PPM A48 6RCK PPM A48 1275 PPM A48 13100 PPM A48 1232 PPM A48 16100 Accessories PPM A48 GSKT PPM A48 WST PPM GA Service options Part number SER PPM IN SER PPM PL SER PPM SLVR SER PPM FYMP PRESSURE 48 SPE Column Rack 1 mL PRESSURE 48 SPE Column Rack 3 mL PRESSURE 48 SPE Column Rack 6 mL PRESSURE 48 Collection Rack 12 X 75 mm PRESSURE 48 Collection Rack 13 x 100 mm PRESSURE 48 Sample Vial Rack 12 x 32 mm PRESSURE 48 Collection Rack 16 x 100 mm PRESSURE 48 Sealing Gasket 48 position PRESSURE 48 Waste Bin Inserts PRESSURE Gas Supply Adapter for all models Descript
161. preparation applications e Process up to 20 columns simultaneously Ordering Information Description Gravity Rack IST Gravity Rack with 16 mm collection tube rack IST Gravity Rack with 19 mm collection tube rack Accessories Universal PTFE stopcock PTFE stopcock needle unit PTFE needle unit Stainless steel needle Stainless steel needle retainer Quantity 10 10 10 20 10 The Biotage IST Gravity Rack is a free standing rack for processing sample preparation columns using gravity The rack is ideal for processing ISOLUTE HM N columns in supported liquid extraction applications The rack is made of high density polyethylene with excellent solvent resistance The unique two part design ensures no cross contamination and easy access to extracts Racks are available to accommodate 16 mm or 19 mm diameter collec tion tubes and are compatible with the whole range of PTFE stopcocks and needle units for additional flow control Part number 123 2016 123 2019 121 0009 121 0001 121 0002 121 0003 121 0004 103 Automated Sample Processing Products RapidTrace Automated High Throughput Solid Phase Extraction RapidTraces SPE Workstation The RapidTrace is a robust automated platform for quickly developing rugged reliable SPE methods in regulated pharmaceutical clinical and forensic laboratories The RapidTrace eliminates SPE bottlenecks so you can realize the full benefits of today s po
162. provide a simple alternative to traditional liquid liquid extraction for complex aqueous matrices such as milk The columns contain a modified form of diatomaceous earth which acts as an inert support for the extraction process The aqueous sample is loaded under gravity and is absorbed onto the support When a suitable water immisci ble solvent is passed through the column the analytes are efficiently desorbed and the extract is collected As the aqueous and solvent phases are never in direct contact emulsion formation a particular problem for fat laden samples is eliminated Ordering information for ISOLUTE HM N columns can be found on page 79 Agrochemical and Food Applications and Products for Agrochemical and Food Analysis ISOLUTE SAX PSA SPE Columns for Interference Removal Remove polar interferences and pigments from plant extracts prior to pesticide residue analysis with ISOLUTE SAX PSA columns Ordering Information ISOLUTE SAX PSA Description Quantity Part number ISOLUTE SAX PSA 1 g 6 mL 30 924 0100 C See Application Note IST1027 for more information Visit www biotage com applications to download AFFINILUTE MIP Biotage now offers AFFINULTE MIPs which use molecularly imprinted technology to selectively clean up food products that have previously been difficult to separate With increasingly low maximum tolerance levels for harmful compounds in food production this selectivity has never been more e
163. r 96 manifold Features and Advantages e Vacuum processing for all 96 well sample preparation applications is compatible with fixed well and modular Array plates e Solvent resistant construction for robust and reliable operation e Separate vacuum control options prevent contamination e Sloping internal base eliminates the need for disposable reservoir trays e Simple design allows for easy cleaning Ordering Information Description Quantity Part number VacMaster 96 Sample Processing Manifold 1 121 9600 without vacuum control Vacuum Control Options VacMaster VCU 1 an independent polypropylene unit with vacuum gauge on off switch and fine controls Use with a laboratory vacuum source VacMaster VCU 2 an independent polypropylene unit with all the controls of the VCU 1 plus an integral vacuum generator The unit converts laboratory compressed air into a vacuum source eliminating the need for a vacuum pump Ordering Information Description Quantity Part number VacMaster VCU 1 1 121 9601 VacMaster VCU 2 1 121 9602 99 VacMaster 96 Sample Processing Manifold Ordering Information VacMaster 96 Accessories and Spare Parts Description Quantity Part number VacMaster 96 replacement gasket 1 121 9612 VacMaster 96 replacement o ring amp 1 121 9613 Collection plate spacer 2 mm for deep well collection plates E 1 121 9614 Array insert 6 mm acetal Eb 1 121 9610 Collection plate spacer 29 mm for shall
164. r separation of chlorinated pesticides The approximate chemical composition is MgO e 3 6 SiO 0 1 OH The particle size range is 150 250 um Ordering Information ISOLUTE FL SPE Columns Part number Description 712 0010 A ISOLUTE FL 100 mg 1 mL 712 0020 A ISOLUTE FL 200 mg 1 mL 712 0020 B ISOLUTE FL 200 mg 3 mL 712 0020 H ISOLUTE FL 200 mg 10 mL 712 0050 B ISOLUTE FL 500 mg 3 mL 712 0050 C ISOLUTE FL 500 mg 6 mL 712 0050 H ISOLUTE FL 500 mg 10 mL 712 0050 L ISOLUTE FL 500 mg 6 mL glass 712 0100 B ISOLUTE FL 1 g 3 mL 712 0100 C ISOLUTE FL 1 g 6 mL 712 0200 D ISOLUTE FL 2 g 15 mL 712 0500 E ISOLUTE FL 5 g 25 mL 712 2000 F ISOLUTE FL 20 g 70 mL Support Documents Applications Matrix 0 Analytes Retention Mechanisms Quantity 100 100 50 50 50 30 50 30 50 30 20 20 16 IST1080 Multi residue Extraction and Clean up of Pesticides from Fruits and Vegetables See pages 170 and 171 for additional technical information about ISOLUTE FL High activity 50 200 um particle size range alumina available in acidic neutral and basic surface pH options The surface of the alumina can absorb molecules by interaction vvith the aluminium metal center hydrogen bonding vvith surface hydroxyl groups or by ion exchange if the surface carries a charge The extent of these different interactions can be enhanced by control of the surface pH by treatment with acidic basic or neutral solutions ISOLUTE AL A Acid vvashing r
165. ranty applies only to the plastic tube frits and labor required for packing and testing the cartridge Biotage will not be liable for any damage to media provided by the Customer that may be caused when Biotage packs such media in accordance with Biotage s standard operating procedures Should any Biotage consumable fail to meet the limited warranty above after being tested in accordance with the applicable Biotage standard operating procedures Biotage will provide at its sole option either a replace ment cartridge or reaction vial If a cartridge it will be packed with the original media or packed with new media at no cost to the Purchaser If such failed cartridge is packed with media provided by the Purchaser Biotage will make a reasonable effort to re pack the original media or pack a replacement cartridge with new media provided by the Purchaser Returns of any Consumable must be authorized in advance Please contact Biotage for a Return Authorization RA number and shipping instructions All claims must be made within thirty 30 days of shipment from Biotage or after the initial test of the cartridge at the Customer s facility whichever is first BIOTAGE MAKES NO OTHER WARRANTY EXPRESSED OR IMPLIED INCLUDING MERCHANTABILITY OR SUITABILITY FOR ANY PURPOSE BIOTAGE WILL NOT BE LIABLE IN ANY EVENT FOR SPECIAL INDIRECT OR CONSEQUENTIAL DAMAGES WHETHER ARISING IN TORT UNDER ANY WARRANTY OR OTHERWISE Trademark Acknowledgem
166. red option for these methods Most commonly used C8 sorbent for the extraction of acidic neutral and basic compounds TN101 112 amp UJ UJ U1 from aqueous matrices Secondary silanol or ionic interactions can be used to enhance 126 extract purity and method robustness for basic compounds Extraction from aqueous matrix using non polar interactions for acidic neutral and basic TN101 compounds Extraction from aqueous matrix using non polar interactions for acidic neutral and basic TN101 amp 112 compounds Secondary silanol or ionic interactions can be used to enhance extract purity and method robustness for basic compounds For more information on non polar SPE procedures see the QuickStart Guide page 173 185 o UJ N UJ UJ For in depth technical information on the use of non polar sorbents refer to the Chemistry Data Sheets listed in the table above 157 Non Polar Sorbents ISOLUTE non polar silica based sorbents provide hydrophobic interactions from the highly retentive ISOLUTE C18 to the less retentive ISOLUTE C2 The non polar sorbents can retain a range of compounds that vary in hydrophobic character The sorbents are available in both non endcapped and in certain cases endcapped versions to meet the needs of particular applications Non endcapped sorbents are particularly useful vvhen utilizing secondary silanol interactions for extraction of basic drugs compounds see pages 174 1
167. regated Waste Lines Four segregated output lines allow for easy separation of aqueous organic and biological waste Useful to simplify hazardous waste handling and reduce disposal costs e Simple and Reliable Simple rugged design with a minimum of moving parts and easily accessible consumables allows for a robust system with minimum dovvn time m Reagent mixing chamber allovvs automated online reagent blending in the method rl Closed fluid path system with segregated waste lines maintain integrity of sample ar Positive pressure stepper motor syringe pump delivers precise flow rates Eight solvent reagent lines for maximum methods flexibility Compliance Validation Each unit includes a Validation Manual that allows operators to walk through the validation process of their RapidTrace This ensures that the instrument provides the consistently clean extracts required for today s sophisticated analytical instruments and regulatory bodies The Validation Plan defines the methods and the documentation utilized in the development execution and reporting of a validation for a RapidTrace It addresses installation operation and performance qualification This comprehensive document enables operators to meet GLP GMP and GOP requirements RapidTrace Automated High Throughput Solid Phase Extraction Ordering Information Description Quantity Part number RapidTrace Workstation 1 mL and 3 mL
168. ries and because no safe levels have been determined in food public health concerns still arise As such a zero tolerance level has been established for this antibiotic in foodstuffs It is therefore critical to develop highly selective and sensitive analytical assays to monitor chloramphenicol residues in difficult matrices such as foods The application note for AFFINILUTE MIP Chloramphenicol describes the extraction of chloramphenicol from milk and plasma Description Quantity Part number AFFINILUTE MIP Chloramphenicol 25 mg 10 mL 50 M10 0002 G AFFINILUTE MIP Chloramphenicol 25 mg 3 mL 50 M10 0002 B AFFINILUTE MIP Nitroimidazoles Nitroimidazole antibiotics are used to treat anaerobic bacterial and parasitic infections in animals Whilst prohibition legislation varies from state to state there are restrictions on the use of these compounds in food livestock and permitted levels in food for human consumption The application note for AFFINILUTE MIP Nitroimidazoles describes the extraction of nitroimidazoles from solid tissue samples and liquid samples Description Quantity Part number AFFINILUTE MIP Nitroimidazoles 50 mg 3 mL 50 M34 0005 B AFFINILUTE MIP PAH Polycyclic aromatic hydrocarbons PAHs are chemical compounds containing fused aromatic rings The health hazard of PAHs depends on structure and in particular isomeric form ranging from non toxic to extremely toxic and carcinogenic PAHs can be found in foods where studies have sh
169. rine Urine Plasma Plasma Urine Urine Plasma Plasma Plasma Plasma Plasma Plasma Format ISOLUTE SLE 200 Supported Liquid Extraction plate ISOLUTE SLE 1 mL column ISOLUTE SLE 200 Supported Liquid Extraction plate or ISOLUTE SLE 1 mL column ISOLUTE SLE 200 Supported Liquid Extraction plate ISOLUTE SLE 200 Supported Liquid Extraction plate ISOLUTE SLE 200 Supported Liquid Extraction plate and ISOLUTE SLE 1 mL column ISOLUTE SLE 200 Supported Liquid Extraction plate ISOLUTE SLE 200 Supported Liquid Extraction plate ISOLUTE SLE 200 Supported Liquid Extraction plate ISOLUTE SLE 200 Supported Liquid Extraction plate ISOLUTE SLE 200 Supported Liquid Extraction plate ISOLUTE SLE 200 Supported Liquid Extraction plate ISOLUTE SLE 200 Supported Liquid Extraction plate Part number 820 0200 PO1 820 0140 C 820 0200 PO1 820 0140 C 820 0200 PO1 820 0200 PO1 820 0200 PO1 820 0140 C 820 0200 PO1 820 0200 PO1 820 0200 PO1 820 0200 PO1 820 0200 PO1 820 0200 PO1 820 0200 PO1 Industry Forensic Forensic Clinical Clinical Clinical Forensic Clinical Clinical Clinical Forensic Clinical Clinical Clinical 76 76 76 76 76 76 76 76 76 76 76 76 76 76 76 27 Supported Liquid Extraction ISOLUTE SLE ee 10 Biotage Suppor
170. rine obtained by extracting 5 mL urine spiked at the NIDA cut off level of 100 ng mL with and whole blood Improved flow characteristics for more 1 Amphetamine 2 Methamphetamine 2 22 3 Phencyclidine 4 Cocaine viscous samples reducing the possibility of column plugging s provided by vvider column diameters 127 128 Forensic and Clinical Applications and Products for Forensic and Clinical Analysis Ordering Information Description Quantity Part number ISOLUTE HCX 25 mg 1 mL 1 2 100 902 0002 A ISOLUTE HCX 50 mg 1 mL 2 100 902 0005 A ISOLUTE HCX 130 mg 1 mL 3 100 902 0013 A ISOLUTE HCX 130 mg 3 mL 3 50 902 0013 B ISOLUTE HCX 130 mg 6 mL gt 30 902 0013 C ISOLUTE HCX 130 mg 10 mL 3 50 902 0013 H ISOLUTE HCX 300 mg 6 mL 5 30 902 0030 C ISOLUTE HCX 300 mg 10 mL 6 50 902 0030 H 1 Configuration optimized to achieve minimum elution volumes lt 100 uL with optimized elution solvent 2 Suitable for hydrolysed hair extraction clean up 3 Standard configurations for drugs of abuse confirmation testing up to 5 mL urine sample size 4 Optimized for gravity loading of solvents and urine samples onto the column gt Suitable for equine urine extraction 6 Used to extract 5 10 mL urine when screening for drugs of abuse See page 46 for the complete range of ISOLUTE Confirm HCX SPE products Sample Preparation Methods for Drugs of Abuse A series of well defined protocols using industry standard 130 mg ISOLUTE HCX colu
171. ring heart rate and reducing tremor and as such the International Olympic Committee has banned the use of 8 blockers The application note for AFFINILUTE MIP B blockers describes the extraction of B blockers from urine and other biological fluids as well as water Description Quantity Part number AFFINILUTE MIP blockers 25 mg 10 mL 50 M18 0002 G AFFINILUTE MIP B blockers 25 mg 3 mL 50 M18 0002 B AFFINILUTE MIP Clenbuterol Clenbuterol is a non steroidal B2 adrenergic agonist B receptor used as a decongestant and bronchodilator in the treatment of respiratory disorders e g asthma Like all B receptors its use in food chain livestock is internationally banned The application note for AFFINILUTE MIP Clenbuterol describes the extraction of clenbuterol from calf urine Please note that this product is specific for clenbuterol to capture the entire family of B agonists please see the section relating to AFFINILUTE MIP B agonists above Description Quantity Part number AFFINILUTE MIP Clenbuterol 25 mg 10 mL 50 M01 0002 G gt 00 AFFINILUTE Molecularly Imprinted Polymers AFFINILUTE MIP Chloramphenicol Chloramphenicol is a broad spectrum antibiotic that may be a causative agent for aplastic anemia and certain cancers As a consequence the use of chloramphenicol in food producing livestock has been banned in Europe the US and Canada Chloramphenicol however is still widely available in many food producing count
172. rom biological fluid samples TN116 amp 125 Extraction of basic analytes from aqueous matrix using dual non polar and strong cation TN116 exchange interactions A good alternative to HCX for basic compounds that require more retentive non polar character from the mixed mode sorbent co HCX 5 provides the cleanest extract of all the mixed mode sorbents Ideal choice where the TN116 basic analyte to be extracted has sufficient non polar character to be well retained by the C4 non polar component of the mixed mode sorbent Extraction of quaternary amine and polybasic analytes from aqueous matrix using dual TN129 non polar and weak cation exchange interactions Extraction of acidic analytes from aqueous matrix using dual non polar and strong anion TN127 exchange interactions Suitable for a broad range of acidic compounds H Isolation of compounds from complex mixtures Can also be used for fractionation of acidic basic and neutral compounds from aqueous sample matrix using non polar strong cation and strong anion retention mechanisms For more information on mixed mode SPE procedures see the QuickStart Guide page 174 184 For in depth technical information on how to use ISOLUTE mixed mode sorbents refer to the Chemistry Data Sheets in the table above 163 164 lon Exchange Sorbents Isolate ionizable acidic or basic analytes from aqueous or partially aqueous sample matrices using ion exchange retention me
173. s also an excellent choice TurboVap 96 Deep or Shallow 96 Well Plates The TurboVap 96 has two independent compartments that can accommodate 1 mL or 2 mL standard or deep well plates Each compartment has independent control of gas flow and temperature The flow rate of the gas is determined by the volume of solvent in the plate and the temperature values can be set with the help of an evaporation guide supplied Nozzles are easy to clean and require minimal maintenance Key Applications Genomic and proteomic applications that require concentration of purified extracts The evaporation of DMSO from plates is a common application in addition to HPLC aqueous solvent mixtures of methanol or acetonitrile The Turbovap 96 complements automated 96 well SPE workstations that are widely used for drugs of abuse determination and other pharmaceutical SPE methods General Maintenance and Useful Tips not applicable to TurboVap 96 e The lid should be left open between use to prevent the build up of condensation e De ionized or distilled water should be used to prevent a limescale build up in the water bath and glassvvare should be changed at least once a month If chlorinated solvents are used then frequent water changing is recommended e Clear bath solution provided is used to keep the water clear and free from algae e The siphon pump provided should be used to empty the water quickly and safely Individual brochures and leaflets
174. s Part number Description Quantity 421 0025 R ISOLUTE Array CN EC 25 mg 1 mL wells 100 Pre assembled ISOLUTE Array plates are available To order add the suffix P to the equivalent loose well part number Support Documents TN101 Method Development in Solid Phase Extraction using Non polar ISOLUTE SPE Columns for the Extraction of Aqueous Samples TN102 Method Development in Solid Phase Extraction using Polar ISOLUTE SPE columns for the Extraction of Non aqueous Samples TN126 Method Development in Solid Phase Extraction using Non polar ISOLUTE SPE Columns for the Extraction of Biological Fluid Samples See pages 158 and 159 for additional technical information about ISOLUTE CN EC SL eded 5 4 b 5 m 1 gt gt Applications Matrix Analytes Retention Mechanisms Si O i R SO H Non polar and Cationic Primary Non polar and strong cation exchange Si 0 i CH CH Aqueous TSOLUTE HCX has increased non polar character compared with ISOLUTE HCX 5 and reduced non polar character compared with ISOLUTE HCX 3 ISOLUTE HCX combines C8 Octyl and sulfonic acid functionalities Ordering Information ISOLUTE HCX SPE Columns Part number Description Quantity 902 0002 A ISOLUTE HCX 25 mg 1 mL 100 902 0002 AG ISOLUTE HCX 25 mg 1 mL Tabless 100 902 0005 A ISOLUTE HCX 50 mg 1 mL 100 902 0010 A ISOLUTE HCX 100 mg 1 mL 100 902 0010 B ISOLUTE HCX 100 mg 3
175. sing station Ordering Information Description Quantity Part number ISOLUTE Array base plate 1 120 1000 PO1 ISOLUTE base plate sealing strips strips of 8 50 120 1200 Luer Adaptors to fit any vacuum manifold 25 120 1201 Well Removing Tool 1 120 1202 Deep Well Collection Plates Square well polypropylene collection plates with capacity of 1 or 2 mL per well are available Sealed with the piercable sealing cap see page 90 Ordering Information Description Quantity Part number Collection plate 1 mL 50 121 5202 Collection plate 2 mL 50 121 5203 91 PTR Te Tet oe b Manual Sample Processing Products PRESSURE 48 and PRESSURE 96 Positive Pressure Manifolds Biotage PRESSURE manifolds deliver positive pressure parallel processing for 96 well plates 1 mL 3 mL and 6 mL column formats The systems utilize a consistent uniform flow of positive pressure to move both low and high viscosity liquids through SPE plates and columns Each port of the PRESSURE manifold independently maintains constant pressure increasing the overall reproducibility of analyte recoveries This unique design allows for partially populated racks to be used without sacrificing extraction efficiency Features and Advantages e Highly uniform flow from sample to sample for SPE Protein Precipitation and Supported Liquid Extraction e Improved reproducibility accuracy and extraction efficiency even with viscous
176. ssential Description Quantity Part number AFFINILUTE MIP Clenbuterol 25 mg 10 mL 50 M01 0002 G AFFINILUTE MIP Full B agonist 25 mg 10 mL 50 MO2 0002 G AFFINILUTE MIP Full B agonist 25 mg 3 mL 50 M 2 0002 B AFFINILUTE MTP Triazines 25 mg 10 mL 50 M08 0002 G AFFINILUTE MTP Chloramphenicol 25 mg 10 mL 50 M10 0002 G AFFINILUTE MTP Chloramphenicol 25 mg 3 mL 50 M10 0002 B AFFINILUTE MTP Fluoroquinolones 25 mg 3 mL 50 M69 0002 B AFFINILUTE MIP Nitroimidazoles 50 mg 3 mL 50 M34 0005 B AFFINILUTE MTP PAH 25 mg 3 mL 50 M73 0002 B Ordering information for AFFINILUTE MTPs can be found on pages 15 19 Agrochemical and Food Applications and Products for Agrochemical and Food Analysis ISOLUTE QuEChERS QuEChERS are products specifically designed for the effective clean up of samples from complex matrices QuEChERS stands for Quick Easy Cheap Effective Rugged and Safe which is exactly how they work offering the food scientist a quick and easy way to clean up samples Biotage supplies pre weighed extraction and clean up tubes that conform to the published methods from the AOAC and the European Union QuEcHERS Extraction Tubes available Quantity Part number 15 g QUECHERS AOAC 15 mL Extraction Tube 25 Q0010 15V 10 g QUECHERS EN 15 mL Extraction Tube 25 Q0020 15V Clean Up Tubes AOAC Fruit and Vegetable Clean up Tube 25 Q0030 15V EN Fruit and Vegetable Clean up Tube 25 Q0035 15V AOAC Waxed Fruit and Vegetable Clean up Tube 25 Q0050 15V
177. ster Sample Processing Stations constructed from inert materials avoiding sample interference also provide productivity enhancements for the environmental laboratory Biotage s range of products including analyte specific columns and methodologies and innovative accessories designed specifically to solve environmental sample handling problems are highlighted in this section ISOLUTE ENV SPE columns Efficiently extract polar analytes from water samples using ISOLUTE ENV SPE columns This hyper crosslinked polystyrene divinylbenzene polymer based sorbent has a wettable hydroxyl modified surface with an extremely high area gt 1000 m g The sorbent is optimized for loading large volume samples and high analyte recover ies are achieved when loading at fast flow rates of 60 mL min Please request Chemistry Data Sheet TN109 for method development guidelines using ISOLUTE ENV SPE columns Ordering information for ISOLUTE ENV SPE columns can be found on page 30 EVOLUTE SPE columns EVOLUTE polymer based SPE products are now available in formats optimized for extraction of environmental samples Compared with other polymer based SPE products EVOLUTE extracts give lower ion suppression in LC MS MS EVOLUTE ABN a hydrophobic hydrophilic balanced sorbent for simultaneous extraction and clean up of acidic basic and neutral analytes EVOLUTE CX a mixed mode strong cation exchange sorbent for selective extraction of basic analyt
178. ted Liquid Extraction User Guide ISOLUTE SLE supported liquid extraction is a quick easy and cost effective sample preparation solution for analytical chemistry The Biotage SLE User Guide provides a simple 4 step method development guide which outlines the efficient 3 step sample procedure of loading your sample wait five minutes and elute To request a copy please visit www biotage com 78 Supported Liquid Extraction ISOLUTE HM N Columns for Supported Liquid Extraction ISOLUTE HM N columns contain an inert unprocessed support material for routine supported liquid extraction using gravity flow A range of column sizes is available to match sample volume requirements Ordering Information Description Quantity ISOLUTE HM N 300 uL sample 100 ISOLUTE HM N 1 mL sample 100 ISOLUTE HM N 3 mL sample 100 ISOLUTE HM N 5 mL sample 100 ISOLUTE HM N 10 mL sample 50 ISOLUTE HM N 20 mL sample 50 Note Always use a column of equal or greater capacity than the sample volume The maximum capacity of the column is included in the column description listed in the ordering table Please contact your local Biotage representative or Distributor for alternative configurations that are available through our Part number 800 0040 BM 800 0100 CM 800 0220 DM 800 0350 EM 800 0700 FM 800 1300 FM Custom Manufacturing Service see page 187 189 For support documentation please request TN118 The use of ISOLUTE HM N for Rapid
179. ter and may lead to cleaner extracts ISOLUTE HCX HCX 3 and HCX 5 differ only in their degree of non polar character and can be screened in parallel for optimum extract cleanliness and analyte recovery For extraction of very polar basic drugs EVOLUTE CX is recommended ISOLUTE HAX Mixed mode SPE for Acidic Drugs ISOLUTE HAX is a mixed mode sorbent for acidic analytes The combination of non polar C8 and strong anion exchange functionalities make ISOLUTE HAX ideal for the extraction of a wide range of acidic analytes The generic method includes interference elution steps to remove interferences providing a very clean extract for analysis Mixed Mode Sorbents Selection of ISOLUTE mixed mode SPE sorbents by analyte class Class HAX HCX HCX 3 HCX 5 HCX QS Basic pK gt 11 Polybasic Quaternary amine COptimum sorbent for basic drugs where it is difficult or impossible to eliminate positive charge s using pH 161 Mixed Mode Sorbents Mixed mode Sorbents Description Average Pore Particle Size Diameter Hm EVOLUTE Polymer Based Mixed Mode Sorbents EVOLUTE CX Surface modified polystyrene divinylbenzene amp strong cation 30 50 40 exchange S037 EVOLUTE VVCX Surface modified polystyrene divinylbenzene amp weak cation exchange 30 50 40 COOH COO EVOLUTE AX Surface modified polystyrene divinylbenzene amp strong anion exchange 30 50 40 NR3 EVOLUTE WAX Surface modified polystyrene divinylbenzene
180. th various porosities Columns with Integral Depth Filters All 3 mL to 70 mL SPE columns can be supplied pre fitted with high purity ISOLUTE depth filters for in line removal of particulate material Custom Manufacturing Service High Sensitivity Applications Biotage s high purity standards allow the mafority of applications to use our standard polypropylene columns or 96 vvell plates and polyethylene frits Hovvever vve can custom manufacture SPE or filtration products vvith the following options if required e Polypropylene columns 1 3 or 6 mL fitted with stainless steel frits e Glass columns 6 mL with PTFE frits Glass SPE Columns 6 mL glass ISOLUTE SPE columns fitted with PTFE frits are available for high sensitivity work These glass columns have the same dimensions as standard polypropylene 6 mL columns and are compatible with the AutoTrace SPE Work Station Customer Supplied Sorbents We can pack SPE columns or plates with a sorbent of your choice Contact us to discuss your requirements in detail Quotations To request a quote for any sample preparation product that you cannot find in this catalog or to discuss your custom packing requirements contact our order processing department or your local Biotage Distributor See page 8 for How to Place Your Order Please be ready to provide details about your requirements e Format column or 96 well plate e Column size o polypropylene 1 mL 3 mL 6
181. tics ISOLUTE SLE plates and columns are packed with an optimized grade of diatomaceous earth providing reproducible flow characteristics from sample to sample Aqueous samples and extraction solvents load evenly on to the bed an important feature for automated sample preparation procedures where blockages can lead to loss of valuable samples Higher Analyte Recoveries High extraction efficiency and elimination of emulsion formation deliver higher analyte recoveries and lower detection limits as seen in Figure 2 gt c o gt o o D lt gt Imipramine Trimipramine Nortryptiline Figure 2 Comparison of analyte recovery by ISOLUTE SLE and LLE 75 76 Supported Liquid Extraction Transferable Methods The water immiscible extraction solvents used in LLE can also be used for ISOLUTE SLE procedures Sample pre treatment conditions are also the same meaning existing LLE methods are easily transferable to ISOLUTE SLE reducing method development time Additionally Biotage have shown using a suite of analytes B blockers and NSAIDs that methodologies are scalable between formats See Figure 3 Analyte Recovery 200 uL Sample 400 uLSample 1 mL Sample 2 x 650 uL 3 x 800 UL 2 x 2 5 mL M Oxprenolol M Propranolol gt LL Q 2 o old gt 4 200 uL Sample 400 uLSample 1 mL Sample 2 x 650 uL 3 x 800 uL 2x2 5 mL M
182. tinually developing new applications using EVOLUTE SPE products Visit the applications database www biotage com applications to find the latest information Advanced Polymeric Sorbents for LC MS MS Applications Part number Fixed Well Plates EVOLUTE ABN 600 0010 P01 600 0025 P01 EVOLUTE CX 601 0010 P01 601 0025 P01 EVOLUTE VVCX 602 0010 P01 602 0025 P01 EVOLUTE AX 603 0010 P01 603 0025 P01 EVOLUTE VVAX 604 0010 P01 604 0025 P01 Description EVOLUTE ABN 10 mg Fixed Vvell Plate EVOLUTE ABN 25 mg Fixed Well Plate EVOLUTE CX 10 mg Fixed Well Plate EVOLUTE CX 25 mg Fixed Well Plate EVOLUTE WCX 10 mg Fixed Well Plate EVOLUTE WCX 25 mg Fixed Well Plate EVOLUTE AX 10 mg Fixed Well Plate EVOLUTE AX 25 mg Fixed Well Plate EVOLUTE WAX 10 mg Fixed Well Plate EVOLUTE WAX 25 mg Fixed Well Plate EVOLUTE Array Pre Filled 96 well Plates EVOLUTE ABN 600 0010 RP 600 0025 RP EVOLUTE CX 601 0010 RP 601 0025 RP EVOLUTE Array ABN 10 mg 1 mL Plate EVOLUTE Array ABN 25 mg 1 mL Plate EVOLUTE Array CX 10 mg 1 mL Plate EVOLUTE Array CX 25 mg 1 mL Plate EVOLUTE Array Loose Wells EVOLUTE ABN 600 0010 R 600 0025 R EVOLUTE CX 601 0010 R 601 0025 R EVOLUTE WCX 602 0025 R EVOLUTE AX 603 0025 R EVOLUTE WAX 604 0025 R EVOLUTE Array ABN 10 mg Wells EVOLUTE Array ABN 25 mg Wells EVOLUTE Array CX 10 mg 1 mL Wells EVOLUTE Array CX 25 mg 1 mL Wells EVOLUTE Array WCX 25 mg 1 mL Wells EVOLUTE Array AX 25 mg 1 mL Wells
183. tion of standards Using the guidelines above identify the elution solvent giving highest analyte recovery Note that full recovery should be achieved using 2 10 bed volumes If larger volumes are necessary it is likely that a less retentive sorbent or alternative solvent is required 4 Evaluate the initial procedure established in steps 1 3 for a spiked sample of real sample matrix For example if assay is to be developed for a biological fluid matrix and good recovery of analytes has been achieved from spiked buffer samples move to blank biological fluid spiked with analytes 5 Optimize interference elution solvents to achieve cleanest possible extract without loss of analyte 6 Optimize flow rates for each step to maximize productivity 7 Optimize sorbent bed mass If this can be reduced without loss of analyte benefits in reduction of solvent usage will result This can be particularly important when working with low volume biological fluid samples 8 Validate final method 183 184 QuickStart Guide to SPE Troubleshooting SPE methods The information in this guide provides a starting point for SPE method development However if low or variable analyte recoveries are still a problem once a method has been developed using these guidelines this section outlines some simple strategies for identifying the nature of the problem The most common reasons for low analyte recovery in SPE are e Poor analyt
184. tomated methodologies are available request Application Notes IST1042 and IST1018 respec tively for more information Ordering Information Description Quantity Part number ISOLUTE TPH 1 g 6 mL 30 752 0100 C ISOLUTE O amp G SPE Columns ISOLUTE O amp G columns are designed for extraction of total Oil amp Grease from water samples and allow fractionation of the sample into hexane extractable material HEM and silica gel treated hexane extracted material SGT HEM This eliminates the need for separate liquid liquid extraction and silica column fractionation steps The representative procedure in Application Note IST1005 eb mos cD meets U S EPA 1664 guidelines AutoTrace compatible versions are available Request Application Note IST1005 for more information Ordering Information Description Quantity Part number ISOLUTE O amp G 1 g 6 mL 30 753 0100 CD ISOLUTE O amp G 3 g 70 mL 16 753 0300 FD ISOLUTE Na SO FL SPE Columns Clean up mineral oil extracts with ISOLUTE Na gt 5SO FL SPE columns Designed to remove such interferences as halogenated hydrocarbons Application Note IST1077 complies with the ISO 9377 2 200 method Ordering Information Description Quantity Part number ISOLUTE 50 4 4 g 6 mL 30 976 0400 C 139 140 Environmental Applications and Products for Environmental Analysis ISOLUTE SPE Columns with Integral Depth Filters Designed to trap particulate material during sample loa
185. uments Applications Matrix 0 Analytes Retention Mechanisms Primary Weak cation exchange Quantity 100 100 50 50 50 50 50 30 50 50 30 30 20 20 16 TN102 Method Development in Solid Phase Extraction using Polar ISOLUTE SPE Columns for the Extraction of Non aqueous Samples See pages 168 and 169 for additional technical information about ISOLUTE SI ISOLUTE DIOL 2 3 Dinydroxypropoxypropyl Applications Matrix 0 Analytes Retention Mechanisms SOHO OA O OH OH i OH 50 Non polar and Primary Non polar semi polar Chemical structure of DIOL silane covalently bonded to the surface of a silica particle Ordering Information ISOLUTE Diol SPE Columns Part number Description Quantity 430 0010 A ISOLUTE DIOL 100 mg 1 mL 100 430 0010 B ISOLUTE DIOL 100 mg 3 mL 50 430 0010 G ISOLUTE DIOL 100 mg 10 mL 50 430 0020 B ISOLUTE DIOL 200 mg 3 mL 50 430 0050 B ISOLUTE DIOL 500 mg 3 mL 50 430 0050 C ISOLUTE DIOL 500 mg 6 mL 30 430 0050 H ISOLUTE DIOL 500 mg 10 mL 50 430 0100 C ISOLUTE DIOL 1 g 6 mL 30 Support Documents TN102 Method Development in Solid Phase Extraction using Polar ISOLUTE SPE Columns for the Extraction of Non aqueous Samples See pages 168 and 169 for additional technical information about ISOLUTE DIOL 61 Florisil is a povvdered magnesia silica gel Florisil PR is specially processed to give consistent results when used for sample preparation purposes Tt is activated fo
186. umn drying e Options for VacMaster 10 and VacMaster 20 sample processing manifolds Ordering Information Description Quantity Part number VacMaster 10 Drying Adapter for 1 3 amp 6 mL columns 1 124 1001 VacMaster 20 Drying Adapter for 1 3 amp 6 mL columns 1 124 2001 Drying Adapters for other column sizes are available through our Custom Manufacturing Service see page 187 189 Sample Processing Accessories VacMaster Large Volume Extraction LVE Kit The VacMaster Large Volume Extraction LVE Kit is designed to speed up processing of large volume samples using VacMaster Sample Processing Manifolds PTFE tubing is placed into the sample container and is secured with a tubing clip The other end of the tubing is attached to the ISOLUTE SPE column using a column adaptor When vacuum is applied the sample is drawn through the inert tubing path and through the SPE column Each kit contains PTFE tubing 10 x 1 m lengths with numbered tags tubing clips 10 and column adaptors 10 Features and Advantages e Unattended loading of large volume samples e PTFE components prevent sample contamination Ordering Information Description VacMaster LVE Kit for 1 3 and 6 mL columns g r in ii 354544 44 du VacMaster LVE Kits for other column sizes are available through our Custom Manufacturing Service see page 187 189 IST Gravity Rack Features and Advantages e Rack for gravity flow sample
187. unique design allows for betvveen 1 and 48 columns to be processed vvithout empty ports affecting flovv rates Tabless or flangeless columns should be used for full population and optimum sealing Tabless columns are available from Biotage at the same cost as the equivalent standard column format see page 96 The modular rack system accommodates most popular collection vessels Modular Rack System By using stackable collection racks and extraction racks on the PRESSURE 48 manifold many combinations are possible to accommodate varying methodologies Racks only fit the system in one direction ensuring consistent processing throughout PRESSURE 48 and PRESSURE 96 Positive Pressure Manifolds Ease of Use The intuitive Biotage PRESSURE is easily incorporated into laboratory work flow Simply stack 96 well plates and collection plates for PRESSURE 96 or column rack and collection racks for PRESSURE 48 on the platform tray using the positioning markers and slide the tray under the gasket until it hits the stop Rocker switches pressed simultaneously on the side front of the unit lower the gasket and compress it onto the plates columns This simultaneous depression of the rocker switches is a safety feature that ensures hands are kept clear of moving parts during operation Ordering Information PRESSURE 96 Part number Description PPM 96 PRESSURE 96 Positive Pressure Manifold 96 position Includes 1 x 1 mL 96 well collection
188. us Samples TN112 General Approach to the Extraction of Basic Drugs from Biological Fluids using Non polar Non endcapped Sorbents TN126 Method Development in Solid Phase Extraction using Non polar ISOLUTE SPE Columns for the Extraction of Drugs from Biological Fluid Samples See pages 156 and 157 for additional technical information about ISOLUTE C18 Tabless columns for use with Pressure Positive Pressure Manifolds and other automated SPE systems Other tabless columns are available contact Biotage for details Applications Matrix Analytes Retention Mechanism Aqueous Wide polarity range Primary Non polar Endcapped sorbent to minimize secondary silanol interactions Ordering Information ISOLUTE C18 EC SPE Columns 50 8 CHCH 8 0 9 Chemical structure of 18 silane and trimethyl silyl group covalently bonded to the surface of a silica particle Part number Description Quantity 221 0002 A ISOLUTE C18 EC 25 mg 1 mL 100 221 0005 A ISOLUTE C18 EC 50 mg 1 mL 100 221 0005 G ISOLUTE C18 EC 50 mg 10 mL 50 221 0010 A ISOLUTE C18 EC 100 mg 1 mL 100 221 0010 B ISOLUTE C18 EC 100 mg 3 mL 50 221 0010 G ISOLUTE C18 EC 100 mg 10 mL 50 221 0020 B ISOLUTE C18 EC 200 mg 3 mL 50 221 0020 H ISOLUTE C18 EC 200 mg 10 mL 50 221 0050 B ISOLUTE C18 EC 500 mg 3 mL 50 221 0050 C ISOLUTE C18 EC 500 mg 6 mL 30 221 0050 H ISOLUTE C18 EC 500 mg 10 mL 50 221 0100 B ISOLUTE C18 EC 1 g 3 mL 50 221 0100
189. ve or Distributor for products that are available through our Custom Manufacturing Service see page 187 189 83 Sample Preparation Accessorles 86 Accessorles For SPE and Filtration ISOLUTE Column Adaptors Increase column capacity or fractionate complex mixtures by stacking SPE columns ISOLUTE Column Adaptors attach empty reservoirs filtration columns and depth filter reservoirs to SPE columns Ordering Information Description Quantity Part number PTFE Adaptor 1 amp 6 mL and XL columns A B C G amp H 10 120 1100 PE Adaptor 1 3 amp 6 mL columns A B C 10 120 1101 amp PE Adaptor 15 amp 25 mL columns D E 10 120 1102 PE Adaptor 70 mL column 10 120 1103 ISOLUTE Depth Filter Reservoirs Remove particulate matter from aqueous samples and prevent blockage of the top frit of the SPE column Also allows for separate analysis of particulate bound compounds Ordering Information Description Quantity Part number d ISOLUTE depth filter 6 mL 100 120 1003 CD ISOLUTE depth filter 70 mL 50 120 1009 FD Please contact your local Biotage representative or Distributor for products that are available through our Custom Manufacturing Service see page 187 189 Accessories For SPE and Filtration ISOLUTE Column Caps Seal prepared columns sample loaded columns or immunoaffinity columns for transportation Ordering Information Descr
190. vortex mixing in automated systems e Fixed well plates allow for routine high throughput applications e Modular Array plate format allows for method development and variable sample numbers Apply crash Precipitation Apply solvent occurs no vacuum vortex mixing required Protein is retained by depth filter system Optimized filtration system Vacuum Collect purified Bottom frit prevents dripping filtrate Figure 1 Solvent first protein precipitation procedure 82 ISOLUTE PPT Ordering Information Fixed Well Plate Description Quantity Part number ISOLUTE PPT fixed well plate 2 mL 1 120 2040 P01 Modular ISOLUTE Array Wells Description Quantity Part number ISOLUTE Array PPT wells 1 mL 100 120 2040 R ISOLUTE Array PPT wells 2 mL 100 120 2040 T Pre assembled ISOLUTE Array plates are available To order add the suffix P to the equivalent loose well part number ISOLUTE Array Accessories Description Quantity Part number ISOLUTE Array base plate 1 120 1000 PO1 Strip of 8 base plate sealing plugs 50 120 1200 Luer adaptors to fit any standard sample processing manifold 25 120 1201 Well removing tool 1 120 1202 Required when processing a partially populated ISOLUTE Array PPT plate For support documentation please request PS424 ISOLUTE Protein Precipitation Plates and TN130 Sample Preparation using ISOLUTE PPT Protein Precipitation Plates Please contact your local Biotage representati
191. werful analytical instruments The SPE Landscape Advanced bioanalytical techniques like LC MS MS and GC MS are meeting the increasing demand for greater speed and specificity in toxicological screening Conventional sample preparation methods have not kept pace Solid phase extraction SPE in particular an essential step in the analysis of many biomolecules has become a throughput limiting factor in many laboratories The New Solution The RapidTrace is the perfect complement to high speed analytical techniques a powerful high throughput workstation dedicated specifically to SPE In its fully modular configuration a ten module workstation can process up to 100 samples per hour The RapidTrace delivers e More samples per headcount e Faster turnaround of results e Improved quality precision accuracy and sensitivity e Consistency of preparation e Compliance with regulatory requirements e Improved safety e Quick response to peak or unexpected work loads Features and Advantages e NEW Compatible with 1 3 and 6 mL SPE Columns Increased bed height enables the use of a wider range of SPE cartridges including all ISOLUTE and EVOLUTE bed masses allowing for flexibility in methodology e NEW Larger Sample Volumes A new 5 position rack for 40 mL scintillation vials allows for larger sample volumes to be processed often desired for food and environmental applications e NEW Delay Start Feature Easily leave extracted
192. ww biotage com applications some of the applications you ll find include AN 601 Extraction of Tricyclic Antidepressants from Plasma Using ISOLUTE SLE Supported Liquid Extraction Plates AN 602 Extraction of Corticosteroids from Plasma using ISOLUTE SLE Supported Liquid Extraction Plates AN 603 Extraction of Non steroidal Anti inflammatory Drugs NSAIDs from Plasma using ISOLUTE SLE Supported Liquid Extraction Plates Biotage now offer ISOLUTE SLE in column format in order to meet the increasing need for larger sample volumes and automation The columns perform as well as the 96 well plates but allow for greater flexibility so that you can analyze the samples you need to in a way that best suits your laboratory s existing and future applications See page 73 78 185 for more details and ordering information Supported Liquid Extraction User Guide ISOLUTE SLE supported liquid extraction is a quick easy and cost effective sample preparation solution for analytical chemistry The Biotage SLE User Guide provides a simple 4 step method development guide and outlines the simple and efficient 3 step sample processing procedure of load your sample wait 5 minutes and elute To request a copy please visit www biotage com 119 Bioanalytical Applications and Products for Bioanalysis AFFINILUTE MTPs Biotage AFFINILUTE MTPs are a range of SPE phases that offer bioanalytical chemists ultimate selectivity compared vvit
193. y aqueous sample matrices prior to analysis using non polar hydropho bic retention mechanisms Technical information for Biotage s range of non polar sorbents is summarized on these pages Non Polar Sorbents m EVOLUTE Polymer Based Non Polar Sorbents Surface modified polystyrene divinylbenezene ISOLUTE Polymer Based Non Polar Sorbents Average Pore Particle Size Diameter Hm 30 50 40 ENV Hydroxylated polystyrene divinylbenzene Polystyrene divinylbenzene ISOLUTE Silica Based Non Polar Sorbents Octadecyl non endcapped C18 EC 4 Octadecyl endcapped MFC18 Octadecyl monofunctional non endcapped Si CigH37 C C8 Octyl non endcapped 100 Si CigH37 b N U1 U1 U1 O U1 O C8 EC 4 Octyl endcapped Hexyl non endcapped a EC endcapped a chemical process to reduce the concentration of silica surface silanol groups that provide polar and weak cation exchange secondary interactions U1 O 156 Non Polar Sorbents EVOLUTE Sample Preparation Products are a new generation of advanced water vvettable polymeric SPE sorbents for extraction of drugs from biological fluid samples Supported by a generic method EVOLUTE ABN is optimized for the extraction of acidic basic and neutral drugs for bioanalytical sample preparation 50 um EVOLUTE ABN is ideal for extraction of multi analyte suites from aqueous samp

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Biotage&reg; Analytical Sample Preparation

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Biotage® Analytical Sample Preparation